Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,441,383 T→C S475S (AGT→AGC)  ytfM → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,441,3830TC99.7% 1030.2 / NA 330S475S (AGT→AGC) ytfMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (121/207);  total (123/207)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472
                                                                                          |                                                                                         
tGTTACACGCGGCACGCTGGGCTGTATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAt                                                                                         <  1:228795/93‑1 (MQ=255)
tGTTACACGAGACACGCTGAGCTGGATTGAAACAGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAt                                                                                         <  2:7747148/93‑1 (MQ=255)
 gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAtt                                                                                        <  1:606040/93‑1 (MQ=255)
   tACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCg                                                                                      <  1:7178718/93‑1 (MQ=255)
   tACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCg                                                                                      >  1:1625950/1‑93 (MQ=255)
   tACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCg                                                                                      <  2:6511671/93‑1 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGt                                                                                     <  1:4608468/93‑1 (MQ=255)
     cacGCGGCACGCTGGGCTGTATTGAAACCGGTGATTGCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                    <  2:5196287/93‑1 (MQ=255)
     cacGCGGCACGCTGGGCTGGATTGAAGCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                    <  1:6212159/93‑1 (MQ=255)
     cacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                    >  1:1416434/1‑93 (MQ=255)
     cacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGAACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                    >  1:6772988/1‑93 (MQ=255)
      acGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATATGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTgg                                                                                   >  1:948547/1‑93 (MQ=255)
        gcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCt                                                                                 <  1:7598011/93‑1 (MQ=255)
         cgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCta                                                                                <  1:6969795/93‑1 (MQ=255)
          ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtac                                                                               <  2:6350649/93‑1 (MQ=255)
          ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtac                                                                               <  1:1816678/93‑1 (MQ=255)
           gCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtaca                                                                              <  1:7105247/93‑1 (MQ=255)
              cGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaat                                                                           <  1:1212719/93‑1 (MQ=255)
              cGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaat                                                                           <  2:6480274/93‑1 (MQ=255)
               gctgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaata                                                                          <  1:6318945/93‑1 (MQ=255)
               gctgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaata                                                                          <  1:4771307/93‑1 (MQ=255)
                   ggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaatacaaa                                                                      <  1:2777964/93‑1 (MQ=255)
                   ggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaatacaaa                                                                      <  2:6687930/93‑1 (MQ=255)
                    gctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTacaaatacaaat                                                                     <  1:3530895/93‑1 (MQ=255)
                     ctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATc                                                                    <  2:1821818/93‑1 (MQ=255)
                      tggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCt                                                                   <  1:1396739/93‑1 (MQ=255)
                       ggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTa                                                                  <  2:3446327/93‑1 (MQ=255)
                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATc                                                                <  2:3548707/93‑1 (MQ=255)
                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATc                                                                <  2:6148104/93‑1 (MQ=255)
                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATc                                                                <  1:7638451/93‑1 (MQ=255)
                          ttGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                               >  1:6875799/1‑93 (MQ=255)
                           tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                              <  2:1437809/93‑1 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           >  2:4476907/1‑93 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           <  2:6201006/93‑1 (MQ=255)
                                ccGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGa                                                         >  1:432380/1‑93 (MQ=255)
                                  ggTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGaaa                                                       <  1:7770677/93‑1 (MQ=255)
                                  ggTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGaaa                                                       <  2:1242940/93‑1 (MQ=255)
                                  ggTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGaaa                                                       <  1:4898559/93‑1 (MQ=255)
                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      >  2:5138980/1‑93 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     >  1:5876565/1‑93 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     >  1:1291582/1‑93 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     <  1:822299/93‑1 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     <  1:3458850/93‑1 (MQ=255)
                                      aTTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACg                                                   <  1:6609497/93‑1 (MQ=255)
                                       tttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCTGGGGCGACCGCAGTATTCGTGTCTACAATTACAAATCTATCGCGCCGAAATACGc                                                  >  2:7012224/1‑93 (MQ=255)
                                       tttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGc                                                  <  2:2576453/93‑1 (MQ=255)
                                        ttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGcc                                                 <  2:1679053/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  2:875428/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  1:3047346/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              >  1:5558884/1‑93 (MQ=255)
                                            aCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACg                                             >  2:4287603/1‑93 (MQ=255)
                                            aCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACg                                             <  2:2067505/93‑1 (MQ=255)
                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            >  2:3736292/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           >  1:1011377/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           <  2:7191119/93‑1 (MQ=255)
                                                 gTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAc                                        >  2:335259/1‑93 (MQ=255)
                                                   aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                      >  1:7916016/1‑93 (MQ=255)
                                                   aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                      <  1:4109763/93‑1 (MQ=255)
                                                    ccgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTg                                     >  1:4923062/1‑93 (MQ=255)
                                                     cgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGa                                    <  2:5484107/93‑1 (MQ=255)
                                                     cgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGa                                    <  2:1277558/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  2:794224/1‑93 (MQ=255)
                                                        cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 <  1:5519556/93‑1 (MQ=255)
                                                        cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 <  2:1138133/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  1:3839032/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  2:4589715/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  2:4063954/93‑1 (MQ=255)
                                                          gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  1:5934775/1‑93 (MQ=255)
                                                           aTCTGCGTTTCTTCGCCGGGGGCAACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgggg                              <  2:6616125/93‑1 (MQ=255)
                                                            tCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             <  1:7399845/93‑1 (MQ=255)
                                                              tGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                           >  1:727804/1‑93 (MQ=255)
                                                                cGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCg                         <  1:3137762/93‑1 (MQ=255)
                                                                 gTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGa                        >  2:3784646/1‑93 (MQ=255)
                                                                 gTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGa                        <  1:5138248/93‑1 (MQ=255)
                                                                  tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       <  2:5930116/93‑1 (MQ=255)
                                                                  tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       >  1:5368475/1‑93 (MQ=255)
                                                                  tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       <  1:3895941/93‑1 (MQ=255)
                                                                    tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     <  1:5704363/93‑1 (MQ=255)
                                                                      ttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTg                   >  2:5688591/1‑93 (MQ=255)
                                                                       tcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGa                  >  2:7463673/1‑93 (MQ=255)
                                                                       tcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGa                  <  2:2367880/93‑1 (MQ=255)
                                                                          ccGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               >  2:7517963/1‑93 (MQ=255)
                                                                            gggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc             >  2:28366/1‑93 (MQ=255)
                                                                             ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg            >  2:3937128/1‑93 (MQ=255)
                                                                             ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg            <  1:1540331/93‑1 (MQ=255)
                                                                               ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          <  2:1938742/93‑1 (MQ=255)
                                                                               ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          >  1:2627909/1‑93 (MQ=255)
                                                                                gCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAt         <  2:304508/93‑1 (MQ=255)
                                                                                gCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAt         <  1:243793/93‑1 (MQ=255)
                                                                                   gccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      <  1:2164273/92‑1 (MQ=255)
                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  1:7466848/1‑93 (MQ=255)
                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  2:2984896/1‑93 (MQ=255)
                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAAACTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  2:4839561/1‑93 (MQ=255)
                                                                                    ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     >  2:5788919/1‑93 (MQ=255)
                                                                                    ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     >  1:6439825/1‑93 (MQ=255)
                                                                                    ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     >  2:2647376/1‑93 (MQ=255)
                                                                                     cGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTg    <  2:4994593/93‑1 (MQ=255)
                                                                                      gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  2:7254440/1‑93 (MQ=255)
                                                                                      gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  1:5025886/1‑93 (MQ=255)
                                                                                       cAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGa  >  1:2002519/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  >  NC_000913/4441290‑4441476
                                                                                             |                                                                                             
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGC                                                                                               <  SRR4302543.2611853/94‑1 (MQ=60)
TTTTGTTACACGCGGCACGCTGGGCCGGATTGTAACCGGTGATCTCGACATATTACTGCCGGATCTGCGTTTCCTCGCCGGGGGCGACCGCAGT                                                                                               >  SRR4302543.7066914/1‑94 (MQ=60)
 TTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCA                                                                                              >  SRR4302543.2509896/1‑94 (MQ=60)
 TTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATATGCGTTTCTTCGCCGGGGGCGACCGCAGCA                                                                                              >  SRR4302543.806138/1‑94 (MQ=60)
 TTTGTTACACGCGGCACGCTGGGCTGGATGGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCA                                                                                              <  SRR4302543.2209807/94‑1 (MQ=60)
  TTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAT                                                                                             >  SRR4302543.6333785/1‑94 (MQ=60)
  TTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAT                                                                                             <  SRR4302543.3129953/94‑1 (MQ=60)
  TTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAT                                                                                             <  SRR4302543.7268262/94‑1 (MQ=60)
   TGTTACACGCGGCACGCTGGGCTGTATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATT                                                                                            <  SRR4302543.230772/94‑1 (MQ=60)
   TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATT                                                                                            >  SRR4302543.6270431/1‑94 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302543.4829658/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           >  SRR4302543.6490616/1‑94 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302543.611580/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302543.2318132/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGGGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           >  SRR4302543.6602256/1‑94 (MQ=60)
     TTACCCGCGGCACGGTGGTGTGTATTGAAACCGGTGCTTTCGGCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCG                                                                                          <  SRR4302543.1675379/94‑1 (MQ=60)
     TTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCG                                                                                          <  SRR4302543.4931348/94‑1 (MQ=60)
     TTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCG                                                                                          <  SRR4302543.1675380/94‑1 (MQ=60)
     TTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCG                                                                                          >  SRR4302543.1640436/1‑94 (MQ=60)
      TACCCGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.7057195/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.1590644/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.7916831/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.4975046/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.7248395/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302543.7253439/94‑1 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        <  SRR4302543.4650328/94‑1 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        >  SRR4302543.7123679/1‑94 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        >  SRR4302543.1429258/1‑94 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGAACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        >  SRR4302543.6836968/1‑94 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAGCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302543.6270814/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302543.1521450/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302543.7045507/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302543.3884510/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302543.3884509/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATATGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       >  SRR4302543.956831/1‑94 (MQ=60)
          CGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCT                                                                                     <  SRR4302543.1569574/94‑1 (MQ=60)
           GCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTA                                                                                    <  SRR4302543.7670175/94‑1 (MQ=60)
            CGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTAC                                                                                   <  SRR4302543.7035382/94‑1 (MQ=60)
            CGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTAC                                                                                   <  SRR4302543.2265277/94‑1 (MQ=60)
             GGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACA                                                                                  <  SRR4302543.1832972/94‑1 (MQ=60)
             GGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACA                                                                                  <  SRR4302543.540245/94‑1 (MQ=60)
              GCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAA                                                                                 <  SRR4302543.7174261/94‑1 (MQ=60)
               CACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAA                                                                                >  SRR4302543.7197362/1‑94 (MQ=60)
               CACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAA                                                                                <  SRR4302543.738326/94‑1 (MQ=60)
               CACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAA                                                                                >  SRR4302543.5178752/1‑94 (MQ=60)
               CACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGAACGCAGCATTCGTGGCTACAAA                                                                                >  SRR4302543.7380393/1‑94 (MQ=60)
                ACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302543.5090723/94‑1 (MQ=60)
                 CGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATA                                                                              <  SRR4302543.7288681/94‑1 (MQ=60)
                 CGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATA                                                                              <  SRR4302543.1223625/94‑1 (MQ=60)
                  GCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATAC                                                                             <  SRR4302543.6378370/94‑1 (MQ=60)
                  GCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATAC                                                                             <  SRR4302543.4814915/94‑1 (MQ=60)
                    TGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAA                                                                           >  SRR4302543.418779/1‑94 (MQ=60)
                      GGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAT                                                                         <  SRR4302543.2803265/94‑1 (MQ=60)
                      GGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAT                                                                         >  SRR4302543.2936875/1‑94 (MQ=60)
                      GGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAT                                                                         <  SRR4302543.7900471/94‑1 (MQ=60)
                      GGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAT                                                                         <  SRR4302543.312018/94‑1 (MQ=60)
                       GCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATC                                                                        <  SRR4302543.2909909/94‑1 (MQ=60)
                       GCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATC                                                                        <  SRR4302543.3562340/94‑1 (MQ=60)
                        GTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCT                                                                       <  SRR4302543.7247610/94‑1 (MQ=60)
                        CTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCT                                                                       >  SRR4302543.7993826/1‑94 (MQ=60)
                         TGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTA                                                                      <  SRR4302543.1409336/94‑1 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATC                                                                    <  SRR4302543.4975985/94‑1 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATC                                                                    >  SRR4302543.2068757/1‑94 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTAGTGGCTACAAATACAAATCTATC                                                                    <  SRR4302543.1378078/94‑1 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGAATTCGTGGCTACAAATACAAATCTATC                                                                    >  SRR4302543.7679600/1‑94 (MQ=60)
                            ATTGAATCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCCGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302543.3974675/94‑1 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   >  SRR4302543.6940529/1‑94 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302543.7710916/94‑1 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302543.3974676/94‑1 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302543.4427123/94‑1 (MQ=60)
                            ATTGAAACCCGTGATTTCGACAAAGTAGCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302543.2605240/94‑1 (MQ=60)
                             TTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGC                                                                  <  SRR4302543.3719417/94‑1 (MQ=60)
                              TGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCT                                                                 >  SRR4302543.4779501/1‑94 (MQ=60)
                               attcctgctgaaccgctcttccgatctccactctTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTC                                                                <  SRR4302543.3540840/60‑1 (MQ=60)
                                 AACCGGTGATTTCGACGAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCG                                                              <  SRR4302543.2000158/94‑1 (MQ=60)
                                 AACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCG                                                              >  SRR4302543.3513257/1‑94 (MQ=60)
                                 AACCGGTGAGTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCG                                                              <  SRR4302543.6424072/94‑1 (MQ=60)
                                  ACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGA                                                             >  SRR4302543.436431/1‑94 (MQ=60)
                                   GCGGTGATTTCGACAAAGTTACGGCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACGAATACAAATCTATCGCTCCGAA                                                            <  SRR4302543.7506123/94‑1 (MQ=60)
                                   CCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAA                                                            <  SRR4302543.4789979/94‑1 (MQ=60)
                                    CGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAA                                                           >  SRR4302543.1112137/1‑94 (MQ=60)
                                    CGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAA                                                           >  SRR4302543.1968199/1‑94 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302543.7601821/94‑1 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          >  SRR4302543.7477893/1‑94 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302543.7843985/94‑1 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302543.4943209/94‑1 (MQ=60)
                                     CGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302543.3412959/94‑1 (MQ=60)
                                      GTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATA                                                         >  SRR4302543.5930106/1‑94 (MQ=60)
                                      GTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATA                                                         >  SRR4302543.1303080/1‑94 (MQ=60)
                                      GTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATA                                                         >  SRR4302543.7478289/1‑94 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302543.3489835/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302543.829543/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302543.3215536/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302543.6071348/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302543.7827132/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        >  SRR4302543.6350025/1‑94 (MQ=60)
                                         ATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGC                                                      <  SRR4302543.6671791/94‑1 (MQ=60)
                                         ATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGC                                                      >  SRR4302543.6085873/1‑94 (MQ=60)
                                             CGAGAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302543.234028/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302543.5951278/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  >  SRR4302543.5609435/1‑94 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  >  SRR4302543.5609433/1‑94 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  >  SRR4302543.3677656/1‑94 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302543.5470211/94‑1 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302543.3074812/94‑1 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302543.7675686/94‑1 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCGTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302543.2117098/94‑1 (MQ=60)
                                                CAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGT                                               >  SRR4302543.1020325/1‑94 (MQ=60)
                                                 AAAGTTCCTCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTG                                              <  SRR4302543.4462701/94‑1 (MQ=60)
                                                 AAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTG                                              <  SRR4302543.5582767/94‑1 (MQ=60)
                                                 AAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTG                                              <  SRR4302543.13960/94‑1 (MQ=60)
                                                  AAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGA                                             <  SRR4302543.5816238/94‑1 (MQ=60)
                                                   AGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAC                                            >  SRR4302543.5077333/1‑94 (MQ=60)
                                                    GTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACC                                           >  SRR4302543.1695092/1‑94 (MQ=60)
                                                     TACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCT                                          <  SRR4302543.5364663/94‑1 (MQ=60)
                                                     TACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCT                                          >  SRR4302543.6977717/1‑94 (MQ=60)
                                                     TACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCT                                          >  SRR4302543.7990374/1‑94 (MQ=60)
                                                      ACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTG                                         >  SRR4302543.4967915/1‑94 (MQ=60)
                                                      ACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTG                                         <  SRR4302543.4146749/94‑1 (MQ=60)
                                                      ACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTG                                         >  SRR4302543.2799800/1‑94 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATGCAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302543.7671145/94‑1 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302543.1094378/94‑1 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302543.827621/94‑1 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302543.3858515/94‑1 (MQ=60)
                                                           CGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGG                                    <  SRR4302543.2712906/94‑1 (MQ=60)
                                                           CGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGG                                    <  SRR4302543.2051454/94‑1 (MQ=60)
                                                           CGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGG                                    <  SRR4302543.5569689/94‑1 (MQ=60)
                                                            GTATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   <  SRR4302543.6208146/94‑1 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   <  SRR4302543.777361/94‑1 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   <  SRR4302543.884894/94‑1 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   >  SRR4302543.4945960/1‑94 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   >  SRR4302543.5989055/1‑94 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   <  SRR4302543.3872915/94‑1 (MQ=60)
                                                               TCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCC                                <  SRR4302543.7470888/94‑1 (MQ=60)
                                                               TCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCC                                <  SRR4302543.6951681/94‑1 (MQ=60)
                                                                CTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCT                               >  SRR4302543.734344/1‑94 (MQ=60)
                                                                CTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCT                               >  SRR4302543.690915/1‑94 (MQ=60)
                                                                 TGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTC                              >  SRR4302543.4397564/1‑94 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            >  SRR4302543.6908546/1‑94 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            <  SRR4302543.3165984/94‑1 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            >  SRR4302543.6908545/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302543.2287088/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302543.5417077/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           <  SRR4302543.761384/94‑1 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           <  SRR4302543.5184696/94‑1 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302543.1550506/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302543.3174339/1‑94 (MQ=60)
                                                                     TTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAG                          <  SRR4302543.3930345/94‑1 (MQ=60)
                                                                     TTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAG                          <  SRR4302543.5607427/94‑1 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         <  SRR4302543.857897/94‑1 (MQ=60)
                                                                       TCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTT                        <  SRR4302543.5756393/94‑1 (MQ=60)
                                                                       TCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTT                        <  SRR4302543.1284400/94‑1 (MQ=60)
                                                                        TTTCGCCCGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTG                       <  SRR4302543.1298378/94‑1 (MQ=60)
                                                                        CTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTG                       >  SRR4302543.4957787/1‑94 (MQ=60)
                                                                        CTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTG                       >  SRR4302543.1448719/1‑94 (MQ=60)
                                                                          TCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGAT                     <  SRR4302543.6671250/94‑1 (MQ=60)
                                                                           CGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATA                    <  SRR4302543.5435264/94‑1 (MQ=60)
                                                                           CGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATA                    >  SRR4302543.4930946/1‑94 (MQ=60)
                                                                            GCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAA                   >  SRR4302543.3122202/1‑94 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  >  SRR4302543.6713234/1‑94 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  >  SRR4302543.6713233/1‑94 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  >  SRR4302543.7303352/1‑94 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  >  SRR4302543.5646689/1‑94 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 >  SRR4302543.4141644/1‑94 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                <  SRR4302543.2937647/94‑1 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                >  SRR4302543.2640220/1‑94 (MQ=60)
                                                                                GGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGG               <  SRR4302543.2370904/94‑1 (MQ=60)
                                                                                GGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGG               <  SRR4302543.1554127/94‑1 (MQ=60)
                                                                                GGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGG               <  SRR4302543.5498757/94‑1 (MQ=60)
                                                                                 GGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGA              >  SRR4302543.2651690/1‑94 (MQ=60)
                                                                                  GGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAT             >  SRR4302543.6379505/1‑94 (MQ=60)
                                                                                   GCGCCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATC            <  SRR4302543.5806690/94‑1 (MQ=60)
                                                                                   GCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATC            <  SRR4302543.245903/94‑1 (MQ=60)
                                                                                   GCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATC            <  SRR4302543.4229035/94‑1 (MQ=60)
                                                                                     GACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGC          >  SRR4302543.5654726/1‑94 (MQ=60)
                                                                                     GACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGC          >  SRR4302543.7538364/1‑94 (MQ=60)
                                                                                     GACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGC          >  SRR4302543.1337252/1‑94 (MQ=60)
                                                                                      GCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCT         <  SRR4302543.2183666/94‑1 (MQ=60)
                                                                                      ACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCT         >  SRR4302543.6257335/1‑94 (MQ=60)
                                                                                      ACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCT         <  SRR4302543.1829197/94‑1 (MQ=60)
                                                                                      ACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCT         >  SRR4302543.6500498/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302543.5976892/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302543.7954817/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302543.5071627/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAGCGCTGA       >  SRR4302543.5976893/1‑94 (MQ=60)
                                                                                         GCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA      >  SRR4302543.2020501/1‑94 (MQ=60)
                                                                                          CAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAA     >  SRR4302543.6494984/1‑94 (MQ=60)
                                                                                          CAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAA     <  SRR4302543.1420302/94‑1 (MQ=60)
                                                                                          CAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAA     >  SRR4302543.2701531/1‑94 (MQ=60)
                                                                                          CAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAA     <  SRR4302543.1420300/94‑1 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302543.6939298/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302543.4347036/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302543.4647858/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302543.3576853/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    <  SRR4302543.142191/94‑1 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302543.5939746/1‑94 (MQ=60)
                                                                                           AGCATTCATGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    <  SRR4302543.6944716/94‑1 (MQ=60)
                                                                                             GATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  <  SRR4302543.1474314/94‑1 (MQ=60)
                                                                                             CATTCGTGTCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  <  SRR4302543.1474316/94‑1 (MQ=60)
                                                                                             CATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  <  SRR4302543.5186073/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  >  NC_000913/4441290‑4441476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑