Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,619	A→G	V91A (GTG→GCG)	b4256 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,619	0	A	G	99.5%	629.3 / ‑5.2	203	V91A (GTG→GCG)	b4256	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (99/103); total (99/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 206 total aligned reads displayed.

ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAA  >  NC_000913/4476529‑4476708

  ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTc                                                                                       <  2:1073501/93‑1 (MQ=255)

  ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTc                                                                                       >  1:1550421/1‑93 (MQ=255)

   cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTca                                                                                      <  1:3263746/93‑1 (MQ=255)

   cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTca                                                                                      <  1:3524800/93‑1 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                 <  2:3737548/93‑1 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                 >  1:3120417/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                 >  2:1560781/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                 >  2:2670213/1‑93 (MQ=255)

         aaaCAAAACGTACGTGGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCg                                                                                <  1:1175107/93‑1 (MQ=255)

          aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGa                                                                               >  1:147916/1‑93 (MQ=255)

          aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGa                                                                               <  2:3215592/93‑1 (MQ=255)

            cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACa                                                                             >  1:1532047/1‑93 (MQ=255)

            cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACa                                                                             <  2:4292560/93‑1 (MQ=255)

             aaaaCGTACGTTGTTAATGTTTGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                            >  2:79984/1‑93 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTTGCGCAACATCCGCGCTTCACAATCGACAt                                                                            >  2:547355/1‑93 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                            <  2:1821920/93‑1 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                            <  2:3750465/93‑1 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTCCGCGCAACATCCGCGCTTCACAATCGACAt                                                                            <  2:761896/93‑1 (MQ=255)

              aaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATg                                                                           >  2:413717/1‑93 (MQ=255)

              aaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATg                                                                           <  2:4530845/93‑1 (MQ=255)

               aaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATgg                                                                          <  1:2292729/93‑1 (MQ=255)

               aaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATgg                                                                          <  1:1681920/93‑1 (MQ=255)

                 cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGcc                                                                        <  1:3831023/93‑1 (MQ=255)

                 cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGcc                                                                        >  1:2141637/1‑93 (MQ=255)

                  gtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGccc                                                                       >  2:3097360/1‑93 (MQ=255)

                   tacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCTCGCAACATCCGCGCTTCACAATCGACATGGCCCg                                                                      >  2:2095744/1‑93 (MQ=255)

                    acgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGt                                                                     <  2:3318770/93‑1 (MQ=255)

                    acgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGAGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGt                                                                     >  1:2014502/1‑93 (MQ=255)

                     cgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTg                                                                    >  2:3075902/1‑93 (MQ=255)

                      gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGc                                                                   >  1:3577215/1‑93 (MQ=255)

                      gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGc                                                                   >  2:3701841/1‑93 (MQ=255)

                       ttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCa                                                                  >  1:2250123/1‑93 (MQ=255)

                       ttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCa                                                                  >  1:1375074/1‑93 (MQ=255)

                       ttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCa                                                                  >  1:1204938/1‑93 (MQ=255)

                         gttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGc                                                                <  2:2974718/93‑1 (MQ=255)

                         gttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGc                                                                >  2:1205191/1‑93 (MQ=255)

                           tAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCa                                                              >  1:1008788/1‑93 (MQ=255)

                            aaTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGATTCACAATCGACATGGCCCGTGCAGCCaa                                                             >  2:4604222/1‑93 (MQ=255)

                             aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAg                                                            >  1:2410601/1‑93 (MQ=255)

                             aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAg                                                            >  1:4379493/1‑93 (MQ=255)

                                ttCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAgcgc                                                         >  2:362100/1‑93 (MQ=255)

                                 tCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCa                                                        <  2:448291/93‑1 (MQ=255)

                                   gAATGCCCGCGAGTTGAACCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                      <  1:870717/93‑1 (MQ=255)

                                   gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGCGCAGCCAAgcgacta                                                      >  1:913582/1‑89 (MQ=255)

                                    aaTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAg                                                     <  1:4080123/93‑1 (MQ=255)

                                      tGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTc                                                   <  2:3944652/93‑1 (MQ=255)

                                      tGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTc                                                   >  1:1811651/1‑93 (MQ=255)

                                        cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                 >  2:3052800/1‑93 (MQ=255)

                                        cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                 <  1:3932989/93‑1 (MQ=255)

                                         ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGat                                                <  1:96956/93‑1 (MQ=255)

                                         ccGCGAGTTAAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGaa                                                >  2:4039878/1‑92 (MQ=255)

                                          cgcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                               <  1:748747/93‑1 (MQ=255)

                                           gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGatat                                              <  1:2827626/93‑1 (MQ=255)

                                           gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGatat                                              >  1:197745/1‑93 (MQ=255)

                                             gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                            <  1:2580389/93‑1 (MQ=255)

                                             gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                            <  1:452672/93‑1 (MQ=255)

                                              aGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCt                                           <  1:1365777/93‑1 (MQ=255)

                                               gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTc                                          >  2:2005772/1‑93 (MQ=255)

                                               gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTc                                          >  2:2827904/1‑93 (MQ=255)

                                                ttGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGGCAAGCGCATAGTCGATATGCTCa                                         >  1:1751569/1‑93 (MQ=255)

                                                ttGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCa                                         <  2:1463357/93‑1 (MQ=255)

                                                 tGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCCTAGTGGATATTCTCaa                                        >  2:1414183/1‑93 (MQ=255)

                                                   aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg                                      <  2:3165217/93‑1 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                     <  2:162920/93‑1 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                     >  1:4415929/1‑93 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                     <  2:1891632/93‑1 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                     <  2:2047790/93‑1 (MQ=255)

                                                     cacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                    >  1:264744/1‑93 (MQ=255)

                                                         cgGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                <  2:2998251/93‑1 (MQ=255)

                                                            gCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                             <  1:3043650/93‑1 (MQ=255)

                                                            gCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                             <  1:3674065/93‑1 (MQ=255)

                                                            gCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                             <  1:4622314/93‑1 (MQ=255)

                                                             cATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCt                            <  1:2682489/93‑1 (MQ=255)

                                                             cATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCt                            >  2:708745/1‑93 (MQ=255)

                                                              aTTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                           >  1:2170490/1‑93 (MQ=255)

                                                              aTTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                           >  2:4438870/1‑93 (MQ=255)

                                                                  ttttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaa                       <  1:2968901/93‑1 (MQ=255)

                                                                   tttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                      <  2:4428718/93‑1 (MQ=255)

                                                                   tgtAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                      <  2:1795598/91‑1 (MQ=255)

                                                                    ttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAg                     >  1:3403776/1‑93 (MQ=255)

                                                                    ttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAg                     >  2:1654640/1‑93 (MQ=255)

                                                                     tAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgc                    <  1:4337158/93‑1 (MQ=255)

                                                                     tAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgc                    <  2:1544921/93‑1 (MQ=255)

                                                                       gagTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgcgc                  >  2:3505020/1‑93 (MQ=255)

                                                                         gTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCaa                <  2:4573653/93‑1 (MQ=255)

                                                                          ttCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt               <  1:1876797/93‑1 (MQ=255)

                                                                          ttCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt               >  1:4365269/1‑93 (MQ=255)

                                                                           tCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATg              <  1:651704/93‑1 (MQ=255)

                                                                            cgcgCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATgg             <  1:2817813/93‑1 (MQ=255)

                                                                             gcgcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGc            >  1:2376041/1‑93 (MQ=255)

                                                                              cgcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCt           <  1:3692643/93‑1 (MQ=255)

                                                                               gcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCtt          >  1:3387699/1‑93 (MQ=255)

                                                                                 aaCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTcc        >  1:4032174/1‑93 (MQ=255)

                                                                                 aaCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTcc        <  2:591761/93‑1 (MQ=255)

                                                                                  aCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt       <  2:2375076/93‑1 (MQ=255)

                                                                                  aCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt       >  1:1231130/1‑93 (MQ=255)

                                                                                   cATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt      >  2:801432/1‑93 (MQ=255)

                                                                                    aTCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCttt     <  2:2410584/93‑1 (MQ=255)

                                                                                       cgcgCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaa  >  1:4154708/1‑93 (MQ=255)

                                                                                       cgcgCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaa  >  1:4252961/1‑93 (MQ=255)

ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAA  >  NC_000913/4476529‑4476708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  >  NC_000913/4476526‑4476712

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCG                                                                                               >  SRR4302535.3104369/1‑94 (MQ=60)

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCG                                                                                               <  SRR4302535.332101/94‑1 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            >  SRR4302535.1478868/1‑94 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            <  SRR4302535.3872401/94‑1 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            <  SRR4302535.3737460/94‑1 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGATAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            >  SRR4302535.2638208/1‑94 (MQ=60)

    TCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTC                                                                                           >  SRR4302535.2397155/1‑94 (MQ=60)

    TCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTC                                                                                           >  SRR4302535.1561589/1‑94 (MQ=60)

     CCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCA                                                                                          >  SRR4302535.3200426/1‑94 (MQ=60)

     CCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCA                                                                                          <  SRR4302535.3717158/94‑1 (MQ=60)

      CGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCAC                                                                                         <  SRR4302535.3549278/94‑1 (MQ=60)

      CGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCAC                                                                                         <  SRR4302535.3286413/94‑1 (MQ=60)

         CATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAAT                                                                                      <  SRR4302535.1559951/94‑1 (MQ=60)

          ATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATC                                                                                     >  SRR4302535.1836670/1‑94 (MQ=60)

          ATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATC                                                                                     >  SRR4302535.3142146/1‑94 (MQ=60)

            AAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGA                                                                                   >  SRR4302535.148949/1‑94 (MQ=60)

            AAACAAAACGTACGTGGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGA                                                                                   <  SRR4302535.1183495/94‑1 (MQ=60)

             AACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGAC                                                                                  >  SRR4302535.70030/1‑94 (MQ=60)

             AACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGAC                                                                                  <  SRR4302535.1373943/94‑1 (MQ=60)

              ACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACA                                                                                 >  SRR4302535.1543086/1‑94 (MQ=60)

               CAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAT                                                                                <  SRR4302535.2259388/94‑1 (MQ=60)

                AAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATG                                                                               >  SRR4302535.3685715/1‑94 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302535.1696569/94‑1 (MQ=60)

                  AACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGC                                                                             <  SRR4302535.2309128/94‑1 (MQ=60)

                  AACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGC                                                                             <  SRR4302535.1694102/94‑1 (MQ=60)

                   ACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCC                                                                            >  SRR4302535.2157049/1‑94 (MQ=60)

                    CGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCC                                                                           >  SRR4302535.4051672/1‑94 (MQ=60)

                    CGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCC                                                                           <  SRR4302535.3857442/94‑1 (MQ=60)

                     GTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCG                                                                          >  SRR4302535.84901/1‑94 (MQ=60)

                      TACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGAGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGT                                                                         >  SRR4302535.2029012/1‑94 (MQ=60)

                       ACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTG                                                                        >  SRR4302535.3432628/1‑94 (MQ=60)

                        CGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGC                                                                       >  SRR4302535.3602014/1‑94 (MQ=60)

                        CGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGC                                                                       >  SRR4302535.2206633/1‑94 (MQ=60)

                         GTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCA                                                                      >  SRR4302535.1384899/1‑94 (MQ=60)

                         GTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCA                                                                      >  SRR4302535.1213590/1‑94 (MQ=60)

                         GTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCA                                                                      >  SRR4302535.2266148/1‑94 (MQ=60)

                            GTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCC                                                                   >  SRR4302535.2679042/1‑94 (MQ=60)

                            GTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACAGCCGCGCTTCACAATCGACATGGCCCGTGCAGCC                                                                   <  SRR4302535.406671/94‑1 (MQ=60)

                             TTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCA                                                                  >  SRR4302535.1015956/1‑94 (MQ=60)

                              TAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAAAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAA                                                                 >  SRR4302535.1200627/1‑94 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                >  SRR4302535.4409459/1‑94 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                >  SRR4302535.2427954/1‑94 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               >  SRR4302535.4400666/1‑94 (MQ=60)

                                 TGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCG                                                              <  SRR4302535.4310091/94‑1 (MQ=60)

                                   TTCGAATGCCCGCGAGTTGAACACGCGGGCGTTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCA                                                            >  SRR4302535.3102141/1‑94 (MQ=60)

                                    TCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAT                                                           >  SRR4302535.3567172/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATA                                                          >  SRR4302535.233926/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATA                                                          >  SRR4302535.885407/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGCGCAGCCAAGCGacta                                                          >  SRR4302535.920073/1‑90 (MQ=60)

                                      GAATGCCCGCGAGTTGAACCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302535.876883/94‑1 (MQ=60)

                                      GAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302535.449548/94‑1 (MQ=60)

                                      GAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302535.978886/94‑1 (MQ=60)

                                      GAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302535.1154416/94‑1 (MQ=60)

                                      GAATGCCCGCGAGGTGAACACGCGGGCCTTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         >  SRR4302535.36350/1‑94 (MQ=60)

                                       AATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGT                                                        <  SRR4302535.4108033/94‑1 (MQ=60)

                                        ATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGNANAGTC                                                       >  SRR4302535.1820449/1‑94 (MQ=60)

                                        ATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTC                                                       >  SRR4302535.1824750/1‑94 (MQ=60)

                                           CCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGAT                                                    <  SRR4302535.3959833/94‑1 (MQ=60)

                                            CCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATA                                                   <  SRR4302535.97645/94‑1 (MQ=60)

                                            CCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATA                                                   >  SRR4302535.4561226/1‑94 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  >  SRR4302535.199104/1‑94 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  <  SRR4302535.1801605/94‑1 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  <  SRR4302535.754013/94‑1 (MQ=60)

                                              GCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATG                                                 <  SRR4302535.2847691/94‑1 (MQ=60)

                                              GCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATG                                                 <  SRR4302535.937316/94‑1 (MQ=60)

                                                GAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCT                                               <  SRR4302535.2598925/94‑1 (MQ=60)

                                                GAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCT                                               <  SRR4302535.455842/94‑1 (MQ=60)

                                                GAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCT                                               <  SRR4302535.2302212/94‑1 (MQ=60)

                                                 AGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTC                                              <  SRR4302535.1375543/94‑1 (MQ=60)

                                                 AGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTC                                              >  SRR4302535.3001549/1‑94 (MQ=60)

                                                  GTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGGCAAGCGCATAGTCGATATGCTCA                                             >  SRR4302535.1764231/1‑94 (MQ=60)

                                                  GTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCA                                             >  SRR4302535.2839805/1‑94 (MQ=60)

                                                     GAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAG                                          <  SRR4302535.2016157/94‑1 (MQ=60)

                                                      AACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGC                                         >  SRR4302535.4446152/1‑94 (MQ=60)

                                                       ACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCC                                        >  SRR4302535.266582/1‑94 (MQ=60)

                                                        CACGCGGGCATTTTTTTAGAGTTCGCGAAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCAAAGCCCATAGTCGATATGCTCAAA‑CCCA                                      >  SRR4302535.3457730/1‑94 (MQ=60)

                                                         ACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCA                                      >  SRR4302535.218050/1‑94 (MQ=60)

                                                          CGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAA                                     <  SRR4302535.1972368/94‑1 (MQ=60)

                                                            CGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAT                                   >  SRR4302535.253038/1‑94 (MQ=60)

                                                             GGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATG                                  <  SRR4302535.2801277/94‑1 (MQ=60)

                                                             GGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATG                                  <  SRR4302535.541900/94‑1 (MQ=60)

                                                               GCATTTTTTTAGAGTTTGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCTAAATGCT                                >  SRR4302535.4150536/1‑94 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCT                                <  SRR4302535.3064973/94‑1 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCT                                <  SRR4302535.3699505/94‑1 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCT                                <  SRR4302535.4653712/94‑1 (MQ=60)

                                                                CATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGGCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTC                               >  SRR4302535.1224254/1‑94 (MQ=60)

                                                                CATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTC                               >  SRR4302535.2186030/1‑94 (MQ=60)

                                                                CATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTC                               <  SRR4302535.2701676/94‑1 (MQ=60)

                                                                  TTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAT                             <  SRR4302535.2108768/94‑1 (MQ=60)

                                                                     TTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAA                          <  SRR4302535.2989735/94‑1 (MQ=60)

                                                                      TTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAG                         >  SRR4302535.3427467/1‑94 (MQ=60)

                                                                       TTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGC                        <  SRR4302535.3309851/94‑1 (MQ=60)

                                                                       TTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATCGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAACCCCAAATGCTCATAAAGC                        >  SRR4302535.2067453/1‑94 (MQ=60)

                                                                        TAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCG                       <  SRR4302535.1621427/94‑1 (MQ=60)

                                                                        TAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCG                       <  SRR4302535.4366821/94‑1 (MQ=60)

                                                                         AGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGC                      <  SRR4302535.4389397/94‑1 (MQ=60)

                                                                            GTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   >  SRR4302535.4395140/1‑94 (MQ=60)

                                                                            GTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   <  SRR4302535.3425165/94‑1 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  <  SRR4302535.4231670/94‑1 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  <  SRR4302535.1890311/94‑1 (MQ=60)

                                                                              TCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGG                 <  SRR4302535.656251/94‑1 (MQ=60)

                                                                               CGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGC                <  SRR4302535.2837829/94‑1 (MQ=60)

                                                                               CGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGC                >  SRR4302535.2393110/1‑94 (MQ=60)

                                                                                GCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCT               >  SRR4302535.137686/1‑94 (MQ=60)

                                                                                 CGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTT              <  SRR4302535.3718201/94‑1 (MQ=60)

                                                                                 CGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTT              >  SRR4302535.3411269/1‑94 (MQ=60)

                                                                                   CAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCC            >  SRR4302535.4059758/1‑94 (MQ=60)

                                                                                    AACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCT           >  SRR4302535.2939017/1‑94 (MQ=60)

                                                                                    AACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCT           >  SRR4302535.1239933/1‑94 (MQ=60)

                                                                                    AACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCT           <  SRR4302535.443354/94‑1 (MQ=60)

                                                                                       ATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTA        <  SRR4302535.835592/94‑1 (MQ=60)

                                                                                        TCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAA       >  SRR4302535.1536326/1‑94 (MQ=60)

                                                                                         CCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAA      >  SRR4302535.4183153/1‑94 (MQ=60)

                                                                                         CCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAA      >  SRR4302535.4282101/1‑94 (MQ=60)

                                                                                          CGCGCTTCACAATCGACATGGCCCGTGCCGCCAAACGCATAGTCGATATGCTCAAAGCCCAACTGCTCATAAAGCGCAATGGCTTCCTTTAAAA     >  SRR4302535.1872248/1‑94 (MQ=60)

                                                                                           GCGCTTCACAATCGACATGGCCCGTGTAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAA    <  SRR4302535.3451399/94‑1 (MQ=60)

                                                                                            CGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAG   >  SRR4302535.3874596/1‑94 (MQ=60)

                                                                                            CGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAA   <  SRR4302535.3728864/94‑1 (MQ=60)

                                                                                             GCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  <  SRR4302535.3664119/94‑1 (MQ=60)

                                                                                             GCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  <  SRR4302535.3800426/94‑1 (MQ=60)

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  >  NC_000913/4476526‑4476712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`