Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,502,304 (T)5→6 coding (679/1218 nt) b4279 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,502,2991.T99.4% 618.4 / ‑3.8 169coding (674/1218 nt)b4279putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (90/78);  total (90/79)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 191 total aligned reads displayed.

GTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC  >  NC_000913/4502207‑4502386
                                                                                             |                                                                                       
gTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTg                                                                                          <  2:1515860/93‑1 (MQ=255)
gTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTg                                                                                          >  1:3745995/1‑93 (MQ=255)
gTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTg                                                                                          >  1:2657393/1‑93 (MQ=255)
   gATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttt                                                                                      <  1:3067594/93‑1 (MQ=255)
    aTTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑tttt                                                                                     >  2:3252463/1‑93 (MQ=255)
     ttGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttt                                                                                    >  2:65468/1‑93 (MQ=255)
     ttGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttt                                                                                    <  2:603891/93‑1 (MQ=255)
        aaGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttgt                                                                                 >  1:3270427/1‑90 (MQ=255)
         aGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttgtc                                                                                >  1:204373/1‑89 (MQ=255)
         aGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttgtc                                                                                >  1:1969088/1‑89 (MQ=255)
           aTAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTa                                                                               <  1:1442703/93‑1 (MQ=255)
           aTAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTa                                                                               >  1:18527/1‑93 (MQ=255)
            tAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAt                                                                              <  2:1833516/93‑1 (MQ=255)
            tAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAt                                                                              >  1:1301102/1‑93 (MQ=255)
             aaaTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAtt                                                                             >  2:2779619/1‑93 (MQ=255)
              aaTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAttt                                                                            >  2:4263693/1‑93 (MQ=255)
               atatAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTg                                                                           >  2:3014305/1‑93 (MQ=255)
                tataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTgg                                                                          >  1:1274556/1‑93 (MQ=255)
                tataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTgg                                                                          >  2:3809562/1‑93 (MQ=255)
                tataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCCATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTgg                                                                          <  1:1478939/93‑1 (MQ=255)
                 ataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGt                                                                         >  1:2827606/1‑93 (MQ=255)
                 ataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGt                                                                         >  2:1542128/1‑93 (MQ=255)
                 ataAGGATAAATCAACATTTCTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGt                                                                         <  1:4150761/93‑1 (MQ=255)
                  taAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTg                                                                        >  1:2475908/1‑93 (MQ=255)
                      gATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCaaa                                                                    <  1:2398471/93‑1 (MQ=255)
                      gATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCaaa                                                                    <  2:3949206/93‑1 (MQ=255)
                       aTAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAAc                                                                   <  2:3297222/93‑1 (MQ=255)
                        tAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACt                                                                  >  2:2637798/1‑93 (MQ=255)
                        tAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACt                                                                  >  1:460308/1‑93 (MQ=255)
                        tAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACt                                                                  >  2:741563/1‑93 (MQ=255)
                        tAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACt                                                                  >  2:2129439/1‑93 (MQ=255)
                         aaaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTg                                                                 >  2:2561848/1‑93 (MQ=255)
                         aaaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTg                                                                 <  1:1079100/93‑1 (MQ=255)
                         aaaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTg                                                                 <  1:955488/93‑1 (MQ=255)
                          aaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTgg                                                                <  2:926579/93‑1 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             >  1:799903/1‑93 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             >  2:1891791/1‑93 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             <  2:4103735/93‑1 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             <  2:4646422/93‑1 (MQ=255)
                              aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGa                                                            <  1:2453153/93‑1 (MQ=255)
                              aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGa                                                            >  1:2243295/1‑93 (MQ=255)
                                 aTTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaa                                                         <  1:3483589/93‑1 (MQ=255)
                                 aTTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaa                                                         >  1:4305988/1‑93 (MQ=255)
                                  tttttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaaa                                                        >  2:2159020/1‑93 (MQ=255)
                                  tttttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaaa                                                        <  2:632918/93‑1 (MQ=255)
                                  tttttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaaa                                                        <  1:2857862/93‑1 (MQ=255)
                                    tttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAAca                                                      <  2:4438066/93‑2 (MQ=255)
                                      tgtctgtcTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGa                                                    <  1:382633/93‑1 (MQ=255)
                                       gtctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGac                                                   >  2:2338487/1‑93 (MQ=255)
                                       gtctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGac                                                   <  1:1888806/93‑1 (MQ=255)
                                       gtctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGac                                                   >  1:1619499/1‑93 (MQ=255)
                                        tctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGact                                                  >  2:3120614/1‑93 (MQ=255)
                                          tgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactac                                                >  1:4488351/1‑93 (MQ=255)
                                           gtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactact                                               <  2:243295/93‑1 (MQ=255)
                                           gtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactact                                               >  1:3497267/1‑93 (MQ=255)
                                           gtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactact                                               >  1:3364729/1‑93 (MQ=255)
                                            tctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActt                                              >  1:1585607/1‑93 (MQ=255)
                                              ttCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcc                                            >  2:3629619/1‑93 (MQ=255)
                                              ttCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcc                                            >  1:883693/1‑93 (MQ=255)
                                               tCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcct                                           <  2:1129668/93‑1 (MQ=255)
                                                 aGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtccttc                                         >  2:1441497/1‑93 (MQ=255)
                                                 aGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtccttc                                         <  1:556098/93‑1 (MQ=255)
                                                  gAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACttccttcc                                        <  1:1552141/93‑1 (MQ=255)
                                                   aaaaCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTtccttcct                                       >  1:734386/1‑93 (MQ=255)
                                                     aaCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTtccttcctgc                                     >  1:4647457/1‑91 (MQ=255)
                                                      aCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGcc                                    <  1:4561080/93‑1 (MQ=255)
                                                       ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCt                                   <  1:1128291/93‑1 (MQ=255)
                                                       ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCt                                   >  1:1253354/1‑93 (MQ=255)
                                                        cacaTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTcctgcctg                                  >  1:1378264/1‑93 (MQ=255)
                                                         acaTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTgg                                 >  1:1261367/1‑93 (MQ=255)
                                                            tCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAg                              <  2:1641527/93‑1 (MQ=255)
                                                             cTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGa                             <  1:775963/93‑1 (MQ=255)
                                                              tttCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGAt                            <  1:1398029/93‑1 (MQ=255)
                                                                tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATaa                          <  1:2005447/93‑1 (MQ=255)
                                                                 cAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAAt                         <  2:2943301/93‑1 (MQ=255)
                                                                   aTCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATgg                       >  2:2062615/1‑93 (MQ=255)
                                                                    tctcTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGa                      <  2:1083529/93‑1 (MQ=255)
                                                                      tctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGt                    >  1:3927946/1‑93 (MQ=255)
                                                                      tctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGt                    <  1:2926335/93‑1 (MQ=255)
                                                                      tctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGt                    <  2:3493956/93‑1 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                 >  2:2274890/1‑93 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                 <  2:2034222/93‑1 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                 <  2:4483423/93‑1 (MQ=255)
                                                                          tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAt                >  2:3163235/1‑93 (MQ=255)
                                                                          tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAt                <  1:471107/93‑1 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa               <  2:3509771/93‑1 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa               <  2:343452/93‑1 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa               <  2:4123657/93‑1 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa               >  1:2134895/1‑93 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa               <  2:3106719/93‑1 (MQ=255)
                                                                               gTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATATTGGAGTTAATACAgg           >  2:505474/1‑92 (MQ=255)
                                                                                ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTg          <  2:16020/93‑1 (MQ=255)
                                                                                 tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTgg         <  1:4446192/93‑1 (MQ=255)
                                                                                 tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTgg         <  2:2867010/93‑1 (MQ=255)
                                                                                 tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTgg         >  2:1283977/1‑93 (MQ=255)
                                                                                  ttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGt        <  1:2279059/93‑1 (MQ=255)
                                                                                     cTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAt     >  1:2912933/1‑93 (MQ=255)
                                                                                      tCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAtt    >  2:2293755/1‑93 (MQ=255)
                                                                                      tCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAtt    <  2:1683191/93‑1 (MQ=255)
                                                                                        gTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTc  <  1:3515807/93‑1 (MQ=255)
                                                                                             |                                                                                       
GTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC  >  NC_000913/4502207‑4502386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  >  NC_000913/4502211‑4502397
                                                                                              |                                                                                             
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTT                                                                                               <  SRR4302535.2203985/94‑1 (MQ=60)
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTT                                                                                               >  SRR4302535.1458024/1‑94 (MQ=60)
   GAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTtgt                                                                                            >  SRR4302535.3293145/1‑91 (MQ=60)
    AAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTC                                                                                            >  SRR4302535.1983274/1‑94 (MQ=60)
    AAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTC                                                                                            >  SRR4302535.205773/1‑94 (MQ=60)
     AGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCT                                                                                           <  SRR4302535.898908/94‑1 (MQ=60)
     AGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCT                                                                                           >  SRR4302535.4257992/1‑94 (MQ=60)
     AGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCT                                                                                           >  SRR4302535.3449564/1‑94 (MQ=60)
      GATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTA                                                                                          >  SRR4302535.18665/1‑94 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         >  SRR4302535.1310380/1‑94 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         <  SRR4302535.1453024/94‑1 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         >  SRR4302535.881057/1‑94 (MQ=60)
           ATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGG                                                                                     >  SRR4302535.1283654/1‑94 (MQ=60)
           ATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGG                                                                                     <  SRR4302535.2926746/94‑1 (MQ=60)
           ATATAAGAATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGG                                                                                     <  SRR4302535.376747/94‑1 (MQ=60)
            TATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGT                                                                                    >  SRR4302535.2847671/1‑94 (MQ=60)
            TATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCCATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGT                                                                                    <  SRR4302535.1489523/94‑1 (MQ=60)
             ATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTG                                                                                   >  SRR4302535.3066715/1‑94 (MQ=60)
             ATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTG                                                                                   >  SRR4302535.1447934/1‑94 (MQ=60)
             ATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTG                                                                                   >  SRR4302535.2493763/1‑94 (MQ=60)
             ATAAGGATAAATCAACATTTCTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTG                                                                                   <  SRR4302535.4179184/94‑1 (MQ=60)
                 GGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAA                                                                               >  SRR4302535.2941786/1‑94 (MQ=60)
                  GATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAAC                                                                              <  SRR4302535.2415717/94‑1 (MQ=60)
                   ATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACT                                                                             >  SRR4302535.463527/1‑94 (MQ=60)
                    TAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTG                                                                            >  SRR4302535.2504820/1‑94 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           <  SRR4302535.962260/94‑1 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           <  SRR4302535.1086779/94‑1 (MQ=60)
                      AATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGC                                                                          <  SRR4302535.4549559/94‑1 (MQ=60)
                        TCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCG                                                                        >  SRR4302535.805520/1‑94 (MQ=60)
                         CAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGA                                                                       >  SRR4302535.2259249/1‑94 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      >  SRR4302535.4477574/1‑94 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      <  SRR4302535.1301611/94‑1 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      <  SRR4302535.2470833/94‑1 (MQ=60)
                           ACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATA                                                                     >  SRR4302535.79033/1‑94 (MQ=60)
                            CATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAA                                                                    >  SRR4302535.4335448/1‑94 (MQ=60)
                             ATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAA                                                                   <  SRR4302535.3507776/94‑1 (MQ=60)
                             ATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAA                                                                   >  SRR4302535.4607937/1‑94 (MQ=60)
                              TTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAAC                                                                  <  SRR4302535.1267757/94‑1 (MQ=60)
                              TTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAAC                                                                  <  SRR4302535.2878110/94‑1 (MQ=60)
                                  TGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGAC                                                              >  SRR4302535.1631177/1‑94 (MQ=60)
                                  TGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGAC                                                              <  SRR4302535.385281/94‑1 (MQ=60)
                                   GTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACT                                                             <  SRR4302535.3299239/94‑1 (MQ=60)
                                   GTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACT                                                             <  SRR4302535.1197515/94‑1 (MQ=60)
                                   GTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACT                                                             >  SRR4302535.3937270/1‑94 (MQ=60)
                                   GTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACT                                                             <  SRR4302535.1902412/94‑1 (MQ=60)
                                    TCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTA                                                            <  SRR4302535.1946289/94‑1 (MQ=60)
                                     CTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTAC                                                           >  SRR4302535.4519162/1‑94 (MQ=60)
                                     CTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTAC                                                           >  SRR4302535.4407451/1‑94 (MQ=60)
                                     CTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTAC                                                           >  SRR4302535.2446614/1‑94 (MQ=60)
                                      TGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACT                                                          >  SRR4302535.3388140/1‑94 (MQ=60)
                                      TGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACT                                                          <  SRR4302535.1281960/94‑1 (MQ=60)
                                      TGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACT                                                          >  SRR4302535.3521570/1‑94 (MQ=60)
                                       GTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTT                                                         >  SRR4302535.1597047/1‑94 (MQ=60)
                                         CTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCC                                                       >  SRR4302535.889947/1‑94 (MQ=60)
                                          TTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCT                                                      <  SRR4302535.3790640/94‑1 (MQ=60)
                                           TCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTT                                                     <  SRR4302535.2029300/94‑1 (MQ=60)
                                            CAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTC                                                    <  SRR4302535.400281/94‑1 (MQ=60)
                                             AGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCC                                                   <  SRR4302535.559996/94‑1 (MQ=60)
                                              GAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCT                                                  <  SRR4302535.1563322/94‑1 (MQ=60)
                                              GAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCT                                                  >  SRR4302535.739548/1‑94 (MQ=60)
                                               AAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTG                                                 >  SRR4302535.2279476/1‑94 (MQ=60)
                                                AAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGC                                                >  SRR4302535.4678939/1‑94 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                              >  SRR4302535.1262315/1‑94 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                              <  SRR4302535.4592210/94‑1 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                              >  SRR4302535.278654/1‑94 (MQ=60)
                                                   CCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTG                                             <  SRR4302535.1136346/94‑1 (MQ=60)
                                                   CCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTG                                             >  SRR4302535.1388098/1‑94 (MQ=60)
                                                    CACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGG                                            >  SRR4302535.1270392/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGC                                           >  SRR4302535.539090/1‑94 (MQ=60)
                                                       ATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAG                                         >  SRR4302535.2004834/1‑94 (MQ=60)
                                                         CTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGAT                                       <  SRR4302535.781419/94‑1 (MQ=60)
                                                          TTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATA                                      <  SRR4302535.1408010/94‑1 (MQ=60)
                                                           TTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAA                                     <  SRR4302535.1868969/94‑1 (MQ=60)
                                                            TCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAAT                                    <  SRR4302535.2019894/94‑1 (MQ=60)
                                                                TCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAG                                <  SRR4302535.1573055/94‑1 (MQ=60)
                                                                 CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGT                               >  SRR4302535.3954777/1‑94 (MQ=60)
                                                                  TCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTT                              <  SRR4302535.2946964/94‑1 (MQ=60)
                                                                    TATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAA                            >  SRR4302535.2875400/1‑94 (MQ=60)
                                                                     ATGAGCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAT                           <  SRR4302535.3408830/94‑1 (MQ=60)
                                                                      TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATA                          >  SRR4302535.2150277/1‑94 (MQ=60)
                                                                      TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATA                          <  SRR4302535.474404/94‑1 (MQ=60)
                                                                       GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                         >  SRR4302535.2141628/1‑94 (MQ=60)
                                                                       GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                         >  SRR4302535.4673207/1‑94 (MQ=60)
                                                                       GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                         <  SRR4302535.3279980/94‑1 (MQ=60)
                                                                        ATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACA                        >  SRR4302535.1503736/1‑94 (MQ=60)
                                                                           GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTG                     >  SRR4302535.757179/1‑94 (MQ=60)
                                                                           GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTG                     >  SRR4302535.1389195/1‑94 (MQ=60)
                                                                             TTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATTGAGTTAATACAGTGGT                   <  SRR4302535.1720330/94‑1 (MQ=60)
                                                                             TTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGT                   <  SRR4302535.4476688/94‑1 (MQ=60)
                                                                              TTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTC                  <  SRR4302535.2295338/94‑1 (MQ=60)
                                                                               TCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCA                 >  SRR4302535.351653/1‑94 (MQ=60)
                                                                                CCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAT                <  SRR4302535.2414179/94‑1 (MQ=60)
                                                                                CCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAT                >  SRR4302535.2933512/1‑94 (MQ=60)
                                                                                   CGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC             >  SRR4302535.1378714/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302535.3929213/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            <  SRR4302535.3540237/94‑1 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302535.3665439/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302535.3757027/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302535.556405/1‑94 (MQ=60)
                                                                                      CTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAAC          <  SRR4302535.1789351/94‑1 (MQ=60)
                                                                                       tgtTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACT         <  SRR4302535.1226594/91‑1 (MQ=60)
                                                                                        T‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCT       <  SRR4302535.1700428/94‑1 (MQ=60)
                                                                                           TTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGA     >  SRR4302535.3430007/1‑94 (MQ=60)
                                                                                            TTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGAT    <  SRR4302535.3743104/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGGAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302535.3010420/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302535.65960/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302535.2469693/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302535.3825668/94‑1 (MQ=60)
                                                                                              TGTCNATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  <  SRR4302535.1414560/94‑1 (MQ=60)
                                                                                              |                                                                                             
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  >  NC_000913/4502211‑4502397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑