Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,502,350 A→G Y242C (TAC→TGC)  b4279 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,502,3500AG99.4% 570.1 / NA 178Y242C (TAC→TGC) b4279putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (74/103);  minor base T (0/1);  total (74/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTG  >  NC_000913/4502261‑4502439
                                                                                          |                                                                                         
aCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGcc                                                                                         >  2:2658479/1‑93 (MQ=255)
 ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCt                                                                                        <  1:1128291/93‑1 (MQ=255)
 ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCt                                                                                        >  1:1253354/1‑93 (MQ=255)
 ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCt                                                                                        >  1:276721/1‑93 (MQ=255)
  cacaTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTcctgcctg                                                                                       >  1:1378264/1‑93 (MQ=255)
    caTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGc                                                                                     >  1:535350/1‑93 (MQ=255)
      tCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAg                                                                                   <  2:1641527/93‑1 (MQ=255)
      tCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAg                                                                                   >  1:1990501/1‑93 (MQ=255)
       cTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGa                                                                                  <  1:775963/93‑1 (MQ=255)
           cAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAAt                                                                              <  2:2943301/93‑1 (MQ=255)
            aaTCTATATGATCCTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATg                                                                             <  2:2513207/93‑1 (MQ=255)
              tctcTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGa                                                                           <  1:1561801/93‑1 (MQ=255)
              tctcTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGa                                                                           <  2:1083529/93‑1 (MQ=255)
               ctctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAg                                                                          >  2:106903/1‑93 (MQ=255)
                   aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                                                                      <  2:4483423/93‑1 (MQ=255)
                   aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                                                                      <  2:2034222/93‑1 (MQ=255)
                   aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                                                                      >  1:2855173/1‑93 (MQ=255)
                   aTGAGCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTaa                                                                      <  1:3385272/93‑1 (MQ=255)
                    tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAt                                                                     <  1:471107/93‑1 (MQ=255)
                    tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAt                                                                     >  2:3001400/1‑93 (MQ=255)
                    tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAt                                                                     >  2:3163235/1‑93 (MQ=255)
                     gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa                                                                    <  2:3509771/93‑1 (MQ=255)
                     gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa                                                                    <  2:343452/93‑1 (MQ=255)
                     gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa                                                                    <  1:3257346/93‑1 (MQ=255)
                     gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATa                                                                    >  1:2134895/1‑93 (MQ=255)
                      aTCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAc                                                                   >  1:4641741/1‑93 (MQ=255)
                        cGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAg                                                                 >  2:4200400/1‑93 (MQ=255)
                         gTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGt                                                                <  2:57764/93‑1 (MQ=255)
                          ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTg                                                               <  2:3710187/93‑1 (MQ=255)
                          ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTg                                                               >  1:1379359/1‑93 (MQ=255)
                          ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTg                                                               <  2:3507928/93‑1 (MQ=255)
                           tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTgg                                                              <  2:2867010/93‑1 (MQ=255)
                           tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTgg                                                              <  1:4446192/93‑1 (MQ=255)
                            ttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGt                                                             <  1:2279059/93‑1 (MQ=255)
                              ccTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCa                                                           <  1:2396953/93‑1 (MQ=255)
                               cTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAt                                                          <  2:2282244/93‑1 (MQ=255)
                               cTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAt                                                          >  1:2912933/1‑93 (MQ=255)
                                tCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAtt                                                         <  2:1683191/93‑1 (MQ=255)
                                tCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAtt                                                         <  2:638037/93‑1 (MQ=255)
                                  gTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTc                                                       >  2:494019/1‑93 (MQ=255)
                                  gTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTc                                                       <  1:3515807/93‑1 (MQ=255)
                                    ctgttttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCaa                                                     <  1:1776488/89‑1 (MQ=255)
                                       ttttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAActc                                                  <  1:1688209/92‑1 (MQ=255)
                                       ttttttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAActc                                                  >  2:3231553/2‑93 (MQ=255)
                                          tttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtga                                               >  1:3406299/1‑93 (MQ=255)
                                          tttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtga                                               <  2:3975600/93‑1 (MQ=255)
                                          tttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtga                                               >  2:1412516/1‑93 (MQ=255)
                                           ttGTCTATTTGGTGGAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtgat                                              <  1:2989460/93‑1 (MQ=255)
                                           ttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtgat                                              <  1:65468/93‑1 (MQ=255)
                                           ttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtgat                                              <  1:3799477/93‑1 (MQ=255)
                                           ttGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCtgat                                              <  1:2452021/93‑1 (MQ=255)
                                              tCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGAc                                           >  2:2214056/1‑93 (MQ=255)
                                                tATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACaa                                         <  1:501865/93‑1 (MQ=255)
                                                tATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACaa                                         >  1:613768/1‑93 (MQ=255)
                                                tATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACaa                                         <  2:4114388/93‑1 (MQ=255)
                                                 aTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAAt                                        <  2:3273432/93‑1 (MQ=255)
                                                  tttGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATa                                       >  1:3866449/1‑93 (MQ=255)
                                                     ggTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCa                                    >  2:912985/1‑93 (MQ=255)
                                                       tGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAgg                                  <  1:3943765/93‑1 (MQ=255)
                                                        gCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGt                                 <  1:2038441/93‑1 (MQ=255)
                                                         cAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGtt                                <  2:1867944/93‑1 (MQ=255)
                                                         cAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGtt                                <  2:3851307/93‑1 (MQ=255)
                                                          aaaCTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGttt                               >  2:1655190/1‑93 (MQ=255)
                                                           aaCTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTa                              <  1:2359156/93‑1 (MQ=255)
                                                           aaCTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTa                              <  2:4412159/93‑1 (MQ=255)
                                                            aCTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAg                             <  1:3980099/93‑1 (MQ=255)
                                                            aCTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAg                             <  2:2108873/93‑1 (MQ=255)
                                                             cTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAgg                            >  2:1599973/1‑93 (MQ=255)
                                                             cTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAgg                            >  2:780603/1‑93 (MQ=255)
                                                              tGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGa                           >  1:1363335/1‑93 (MQ=255)
                                                               ggCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGaa                          >  2:2465931/1‑93 (MQ=255)
                                                                 ccGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAaca                        <  1:1724142/93‑1 (MQ=255)
                                                                  cGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAacac                       <  1:2818768/93‑1 (MQ=255)
                                                                  cGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAacac                       <  1:435859/93‑1 (MQ=255)
                                                                  cGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAacac                       <  1:4004283/93‑1 (MQ=255)
                                                                   gATAAACGGACTACTTCCTTCCTTCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACt                      <  1:4552878/93‑1 (MQ=255)
                                                                   gATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACt                      <  2:4385786/93‑1 (MQ=255)
                                                                   gATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACt                      >  1:1153624/1‑93 (MQ=255)
                                                                   gATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACt                      >  2:189687/1‑93 (MQ=255)
                                                                     tAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGa                    <  2:3925136/93‑1 (MQ=255)
                                                                     tAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGa                    <  1:3602843/93‑1 (MQ=255)
                                                                     tAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGa                    <  1:736267/93‑1 (MQ=255)
                                                                      aaaCGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGAc                   <  1:4587903/93‑1 (MQ=255)
                                                                      aaaCGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGAc                   <  2:2244510/93‑1 (MQ=255)
                                                                        aCGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAg                 >  1:369209/1‑93 (MQ=255)
                                                                        aCGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAg                 >  2:2334278/1‑93 (MQ=255)
                                                                         cGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTTGGAAAAATGACAgg                >  2:4037870/1‑93 (MQ=255)
                                                                         cGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAgg                >  1:1468232/1‑93 (MQ=255)
                                                                         cGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAgg                >  2:1575161/1‑93 (MQ=255)
                                                                          ggACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGt               >  1:964/1‑93 (MQ=255)
                                                                           gACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTa              <  2:460308/93‑1 (MQ=255)
                                                                             ctactTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACa            >  2:1189163/1‑93 (MQ=255)
                                                                              tactTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGAGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACaa           >  1:3124030/1‑93 (MQ=255)
                                                                                cttccttcCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAata         <  1:1075233/93‑1 (MQ=255)
                                                                                 ttccttccTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAatat        <  1:991309/93‑1 (MQ=255)
                                                                                 ttccttccTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAatat        <  2:546746/93‑1 (MQ=255)
                                                                                  tccttcctGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATAtt       <  2:4161876/93‑1 (MQ=255)
                                                                                    cttcctGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATAtttt     <  2:3307102/93‑1 (MQ=255)
                                                                                     ttcctGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATAttttt    >  1:3089406/1‑93 (MQ=255)
                                                                                      tcctGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATAtttttt   >  2:2011332/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTG  >  NC_000913/4502261‑4502439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGT  >  NC_000913/4502258‑4502441
                                                                                             |                                                                                           
AAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTG                                                                                             >  SRR4302535.2279476/1‑94 (MQ=60)
 AAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGC                                                                                            >  SRR4302535.4678939/1‑94 (MQ=60)
   ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                                                                          >  SRR4302535.278654/1‑94 (MQ=60)
   ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                                                                          >  SRR4302535.1262315/1‑94 (MQ=60)
   ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCT                                                                                          <  SRR4302535.4592210/94‑1 (MQ=60)
    CCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTG                                                                                         <  SRR4302535.1136346/94‑1 (MQ=60)
    CCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTG                                                                                         >  SRR4302535.1388098/1‑94 (MQ=60)
     CACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGG                                                                                        >  SRR4302535.1270392/1‑94 (MQ=60)
      ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGC                                                                                       >  SRR4302535.539090/1‑94 (MQ=60)
        ATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAG                                                                                     >  SRR4302535.2004834/1‑94 (MQ=60)
          CTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGAT                                                                                   <  SRR4302535.781419/94‑1 (MQ=60)
           TTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATA                                                                                  <  SRR4302535.1408010/94‑1 (MQ=60)
            TTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAA                                                                                 <  SRR4302535.1868969/94‑1 (MQ=60)
             TCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAAT                                                                                <  SRR4302535.2019894/94‑1 (MQ=60)
                 TCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAG                                                                            <  SRR4302535.1573055/94‑1 (MQ=60)
                  CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGT                                                                           >  SRR4302535.3954777/1‑94 (MQ=60)
                   TCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTT                                                                          <  SRR4302535.2946964/94‑1 (MQ=60)
                     TATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAA                                                                        >  SRR4302535.2875400/1‑94 (MQ=60)
                      ATGAGCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAAT                                                                       <  SRR4302535.3408830/94‑1 (MQ=60)
                       TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATA                                                                      <  SRR4302535.474404/94‑1 (MQ=60)
                       TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATA                                                                      >  SRR4302535.2150277/1‑94 (MQ=60)
                        GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                                                                     >  SRR4302535.4673207/1‑94 (MQ=60)
                        GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                                                                     >  SRR4302535.2141628/1‑94 (MQ=60)
                        GATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATAC                                                                     <  SRR4302535.3279980/94‑1 (MQ=60)
                         ATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACA                                                                    >  SRR4302535.1503736/1‑94 (MQ=60)
                            GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTG                                                                 >  SRR4302535.757179/1‑94 (MQ=60)
                            GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTG                                                                 >  SRR4302535.1389195/1‑94 (MQ=60)
                              TTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATTGAGTTAATACAGTGGT                                                               <  SRR4302535.1720330/94‑1 (MQ=60)
                              TTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGT                                                               <  SRR4302535.4476688/94‑1 (MQ=60)
                               TTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTC                                                              <  SRR4302535.2295338/94‑1 (MQ=60)
                                TCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCA                                                             >  SRR4302535.351653/1‑94 (MQ=60)
                                 CCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAT                                                            <  SRR4302535.2414179/94‑1 (MQ=60)
                                 CCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCAT                                                            >  SRR4302535.2933512/1‑94 (MQ=60)
                                    CGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC                                                         >  SRR4302535.1378714/1‑94 (MQ=60)
                                     GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA                                                        >  SRR4302535.3757027/1‑94 (MQ=60)
                                     GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA                                                        >  SRR4302535.3665439/1‑94 (MQ=60)
                                     GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA                                                        <  SRR4302535.3540237/94‑1 (MQ=60)
                                     GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA                                                        >  SRR4302535.3929213/1‑94 (MQ=60)
                                     GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA                                                        >  SRR4302535.556405/1‑94 (MQ=60)
                                       CTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAAC                                                      <  SRR4302535.1789351/94‑1 (MQ=60)
                                        tgtTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACT                                                     <  SRR4302535.1226594/91‑1 (MQ=60)
                                         T‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCT                                                   <  SRR4302535.1700428/94‑1 (MQ=60)
                                            TTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGA                                                 >  SRR4302535.3430007/1‑94 (MQ=60)
                                             TTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGAT                                                <  SRR4302535.3743104/94‑1 (MQ=60)
                                              TTGTCTATTTGGTGGAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG                                               <  SRR4302535.3010420/94‑1 (MQ=60)
                                              TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG                                               <  SRR4302535.65960/94‑1 (MQ=60)
                                              TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG                                               <  SRR4302535.3825668/94‑1 (MQ=60)
                                              TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG                                               <  SRR4302535.2469693/94‑1 (MQ=60)
                                               TGTCNATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA                                              <  SRR4302535.1414560/94‑1 (MQ=60)
                                                  CTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAA                                           >  SRR4302535.2433873/1‑94 (MQ=60)
                                                  CTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAA                                           >  SRR4302535.1155724/1‑94 (MQ=60)
                                                  CTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAA                                           >  SRR4302535.618034/1‑94 (MQ=60)
                                                   TATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAAT                                          <  SRR4302535.730986/94‑1 (MQ=60)
                                                   TATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAAT                                          <  SRR4302535.505377/94‑1 (MQ=60)
                                                    ATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATA                                         >  SRR4302535.3893037/1‑94 (MQ=60)
                                                    ATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATA                                         >  SRR4302535.2910951/1‑94 (MQ=60)
                                                     TTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAG                                        <  SRR4302535.3048189/94‑1 (MQ=60)
                                                      TTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGA                                       >  SRR4302535.3130117/1‑94 (MQ=60)
                                                         GTGCAGACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGG                                    <  SRR4302535.534947/94‑1 (MQ=60)
                                                         GTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGG                                    >  SRR4302535.3606292/1‑94 (MQ=60)
                                                          TGCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGT                                   <  SRR4302535.3970692/94‑1 (MQ=60)
                                                           GCAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTT                                  <  SRR4302535.2053120/94‑1 (MQ=60)
                                                            CAAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTT                                 <  SRR4302535.2715915/94‑1 (MQ=60)
                                                             AAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTA                                >  SRR4302535.2394937/1‑94 (MQ=60)
                                                             AAACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTA                                >  SRR4302535.1608967/1‑94 (MQ=60)
                                                              AACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGCTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAG                               >  SRR4302535.3409179/1‑94 (MQ=60)
                                                              AACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAG                               <  SRR4302535.3864109/94‑1 (MQ=60)
                                                              AACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAG                               <  SRR4302535.2376085/94‑1 (MQ=60)
                                                              AACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAG                               <  SRR4302535.4089442/94‑1 (MQ=60)
                                                               ACTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGG                              <  SRR4302535.4007293/94‑1 (MQ=60)
                                                                CTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGA                             >  SRR4302535.1373085/1‑94 (MQ=60)
                                                                CTGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGA                             <  SRR4302535.965699/94‑1 (MQ=60)
                                                                 TGGCCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAA                            <  SRR4302535.401598/94‑1 (MQ=60)
                                                                    CCGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACAC                         <  SRR4302535.1736613/94‑1 (MQ=60)
                                                                     CGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACT                        <  SRR4302535.438896/94‑1 (MQ=60)
                                                                     CGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACT                        <  SRR4302535.4031676/94‑1 (MQ=60)
                                                                     CGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACT                        >  SRR4302535.1161871/1‑94 (MQ=60)
                                                                     CGATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACT                        <  SRR4302535.2838789/94‑1 (MQ=60)
                                                                      GATAAACGGACTACTTCCTTCCTTCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTG                       <  SRR4302535.4583988/94‑1 (MQ=60)
                                                                      GATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTG                       <  SRR4302535.616699/94‑1 (MQ=60)
                                                                       ATAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTCGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGA                      <  SRR4302535.3318908/94‑1 (MQ=60)
                                                                        TAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGAC                     <  SRR4302535.3627843/94‑1 (MQ=60)
                                                                        TAAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGAC                     <  SRR4302535.741444/94‑1 (MQ=60)
                                                                         AAACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACA                    <  SRR4302535.4619142/94‑1 (MQ=60)
                                                                          AACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAG                   >  SRR4302535.371757/1‑94 (MQ=60)
                                                                          AACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAG                   <  SRR4302535.2258916/94‑1 (MQ=60)
                                                                           ACGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGG                  >  SRR4302535.1478751/1‑94 (MQ=60)
                                                                           ACGGACTACTACCTTCCTGACTGGCAGCTAATGGAGTTAATAAAGTGATAATATCAACTCTGATGACAAAAGCAGGTTTAGGAACACTGACAGG                  <  SRR4302535.4718106/94‑1 (MQ=53)
                                                                            CGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGT                 >  SRR4302535.975/1‑94 (MQ=60)
                                                                            CGGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGT                 <  SRR4302535.280268/94‑1 (MQ=60)
                                                                             GGACTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTA                >  SRR4302535.4530784/1‑94 (MQ=60)
                                                                                CTACTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGAGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAA             >  SRR4302535.3145788/1‑94 (MQ=60)
                                                                                   CTTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATAT          <  SRR4302535.1082875/94‑1 (MQ=60)
                                                                                    TTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATT         <  SRR4302535.998343/94‑1 (MQ=60)
                                                                                    TTCCTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATT         >  SRR4302535.493634/1‑94 (MQ=60)
                                                                                       CTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTT      >  SRR4302535.3110923/1‑94 (MQ=60)
                                                                                       CTTCCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTT      <  SRR4302535.977332/94‑1 (MQ=60)
                                                                                          CCTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGG   <  SRR4302535.4401761/94‑1 (MQ=60)
                                                                                           CTGCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGT  <  SRR4302535.1539726/94‑1 (MQ=60)
                                                                                             |                                                                                           
AAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGT  >  NC_000913/4502258‑4502441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑