Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,511,365 A→G L173P (CTT→CCT)  fecB ← FecB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,511,3650AG99.5% 617.7 / NA 198L173P (CTT→CCT) fecBFecB
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (110/87);  total (110/88)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454
                                                                                          |                                                                                         
gCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgc                                                                                         <  2:646826/93‑1 (MQ=255)
 ccAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgct                                                                                        <  2:1719062/93‑1 (MQ=255)
     cACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgatggct                                                                                    >  1:3216210/1‑93 (MQ=255)
      aCGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggctg                                                                                   >  1:3875053/1‑93 (MQ=255)
      aCGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggctg                                                                                   >  1:1266257/1‑93 (MQ=255)
          tGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGccc                                                                               >  2:4296807/1‑93 (MQ=255)
           gCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCa                                                                              >  1:435600/1‑93 (MQ=255)
             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            >  1:2994016/1‑93 (MQ=255)
             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            <  2:196468/93‑1 (MQ=255)
               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          >  2:4496032/1‑93 (MQ=255)
               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          <  1:393072/93‑1 (MQ=255)
                tCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcg                                                                         <  1:1023473/93‑1 (MQ=255)
                tCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcg                                                                         >  2:3622348/1‑93 (MQ=255)
                 ccAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcgc                                                                        >  1:4598493/1‑93 (MQ=255)
                    ggTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCAt                                                                     >  1:407989/1‑93 (MQ=255)
                      tctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATcc                                                                   <  1:4558925/93‑1 (MQ=255)
                      tctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATcc                                                                   <  1:2790311/93‑1 (MQ=255)
                       ctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCct                                                                  <  2:1479921/93‑1 (MQ=255)
                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 <  2:2295795/93‑1 (MQ=255)
                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 >  1:3431054/1‑93 (MQ=255)
                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 <  2:1507701/93‑1 (MQ=255)
                        tcCCGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATGctc                                                                 >  2:3656453/1‑93 (MQ=255)
                          cTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctc                                                               <  1:2678007/93‑1 (MQ=255)
                          cTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctc                                                               <  2:1822198/93‑1 (MQ=255)
                           tGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctct                                                              >  1:1862176/1‑93 (MQ=255)
                           tGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctct                                                              >  1:4257832/1‑93 (MQ=255)
                            gAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCCCTGCGCCATCCTCTCtt                                                             >  2:3988695/1‑93 (MQ=255)
                             aGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCttt                                                            <  1:3949121/93‑1 (MQ=255)
                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCCCGCGTGTCCCTTTGGGGGGCTGGCTGGCCCCCTGCGCCATCCCCTCtttt                                                           >  1:3312699/1‑92 (MQ=255)
                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCCTCCTCTCTTTa                                                           >  1:2471169/1‑93 (MQ=255)
                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCtttt                                                           >  2:4179923/1‑92 (MQ=255)
                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  2:844570/1‑93 (MQ=255)
                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  2:2581152/1‑93 (MQ=255)
                                aTGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtg                                                         >  2:454254/1‑93 (MQ=255)
                                aTGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtg                                                         <  1:295093/93‑1 (MQ=255)
                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  2:4660854/93‑1 (MQ=255)
                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        >  2:1245176/1‑93 (MQ=255)
                                  gCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgtt                                                       >  1:2516747/1‑93 (MQ=255)
                                  gCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgtt                                                       <  1:1589533/93‑1 (MQ=255)
                                    aGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgttgt                                                     >  2:282998/1‑93 (MQ=255)
                                     ggTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATgttgtt                                                    >  2:2948716/1‑93 (MQ=255)
                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGTGCCATCCTCTCTTTATGTTGTTcc                                                  >  2:1605632/1‑93 (MQ=255)
                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTcc                                                  <  2:3199981/93‑1 (MQ=255)
                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTcc                                                  <  1:1651594/93‑1 (MQ=255)
                                        tGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCa                                                 <  1:614560/93‑1 (MQ=255)
                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                >  2:1912736/1‑93 (MQ=255)
                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                >  2:3884445/1‑93 (MQ=255)
                                          aaTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGa                                               <  2:2082674/93‑1 (MQ=255)
                                           aTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGAc                                              >  1:1179705/1‑93 (MQ=255)
                                            ttGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACg                                             >  1:3605739/1‑93 (MQ=255)
                                            ttGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACg                                             >  1:4486186/1‑93 (MQ=255)
                                              gCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTg                                           >  2:555050/1‑93 (MQ=255)
                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  1:720087/1‑93 (MQ=255)
                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  1:1910576/1‑93 (MQ=255)
                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  2:2646430/1‑93 (MQ=255)
                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        >  1:3131212/1‑93 (MQ=255)
                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        <  2:2434682/93‑1 (MQ=255)
                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        >  1:1992166/1‑93 (MQ=255)
                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  2:3407894/1‑93 (MQ=255)
                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  2:4222590/1‑93 (MQ=255)
                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  1:3651225/1‑93 (MQ=255)
                                                    cgcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCa                                     <  1:4348506/93‑1 (MQ=255)
                                                    cgcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCa                                     <  1:311709/93‑1 (MQ=255)
                                                     gcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAt                                    >  2:2803124/1‑93 (MQ=255)
                                                      cgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtc                                   >  1:1975968/1‑93 (MQ=255)
                                                      cgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtc                                   >  1:775399/1‑93 (MQ=255)
                                                       gTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtct                                  >  1:4469823/1‑93 (MQ=255)
                                                        tGATGTGCCAAAGGCCACGCGTGTGCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 >  2:4517993/1‑93 (MQ=255)
                                                        tGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 >  2:1558209/1‑93 (MQ=255)
                                                          aTGTGCTAAAGGCCACGCGTGTCCCTTTGGGAGGCTTGCTGGCCCCCTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               <  2:2615760/93‑1 (MQ=255)
                                                          aTGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               >  1:1006278/1‑93 (MQ=255)
                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  1:3908809/93‑1 (MQ=255)
                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             >  1:4173687/1‑93 (MQ=255)
                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  1:766325/93‑1 (MQ=255)
                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  2:2893884/93‑1 (MQ=255)
                                                              gCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtt                           >  1:1077867/1‑93 (MQ=255)
                                                                cAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCTCtttt                         >  2:1188803/1‑93 (MQ=255)
                                                                cAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtttt                         >  1:959565/1‑93 (MQ=255)
                                                                 aaaGGCCGCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  1:229114/93‑1 (MQ=255)
                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGTTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  1:827830/93‑1 (MQ=255)
                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        >  2:34807/1‑93 (MQ=255)
                                                                  aaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTa                       <  1:353103/93‑1 (MQ=255)
                                                                   aGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAc                      >  2:3820524/1‑93 (MQ=255)
                                                                   aGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAc                      >  1:1348035/1‑93 (MQ=255)
                                                                    ggCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAcc                     >  2:1934273/1‑93 (MQ=255)
                                                                     gCCACCCGTTTCCCTTTGGGAGGCTGGCTGGGCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGCTGTGCCTGCATCTCTCGCttttttact                    >  2:3943963/1‑89 (MQ=255)
                                                                      ccACGCGTGTCCCTTTGGGAGTCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACcca                   <  2:756014/93‑1 (MQ=255)
                                                                      ccACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACcca                   <  2:28192/93‑1 (MQ=255)
                                                                        aCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccacc                 >  1:947596/1‑93 (MQ=255)
                                                                         cgcgTGTCCCTTTGGGAGGCTGGCGGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccacca                <  1:1215572/93‑1 (MQ=255)
                                                                          gcgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAt               <  2:2456043/93‑1 (MQ=255)
                                                                           cgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAtt              <  2:375379/93‑1 (MQ=255)
                                                                               tCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGc          <  1:2211265/93‑1 (MQ=255)
                                                                                cccTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGcc         <  2:250736/93‑1 (MQ=255)
                                                                                cccTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGcc         >  1:3481276/1‑93 (MQ=255)
                                                                                  cTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCga       <  2:3277730/93‑1 (MQ=255)
                                                                                     tGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatga    <  1:214532/93‑1 (MQ=255)
                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   <  1:1322394/93‑1 (MQ=255)
                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   >  2:2135891/1‑93 (MQ=255)
                                                                                       ggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATa  <  2:1009729/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  >  NC_000913/4511272‑4511457
                                                                                             |                                                                                            
GAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAG                                                                                              <  SRR4302535.2556977/94‑1 (MQ=60)
GAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAG                                                                                              <  SRR4302535.1561505/94‑1 (MQ=60)
GAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAG                                                                                              >  SRR4302535.829165/1‑94 (MQ=60)
 AGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGG                                                                                             >  SRR4302535.4083753/1‑94 (MQ=60)
   GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCT                                                                                           <  SRR4302535.3951636/94‑1 (MQ=60)
   GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCT                                                                                           <  SRR4302535.5764/94‑1 (MQ=60)
     CAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGG                                                                                         >  SRR4302535.315042/1‑94 (MQ=60)
      AGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGC                                                                                        >  SRR4302535.1244653/1‑94 (MQ=60)
       GCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGATGGCT                                                                                       >  SRR4302535.3238488/1‑94 (MQ=60)
        CACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTG                                                                                      <  SRR4302535.2204139/94‑1 (MQ=60)
        CACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTG                                                                                      >  SRR4302535.3901676/1‑94 (MQ=60)
        CACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTG                                                                                      >  SRR4302535.1275314/1‑94 (MQ=60)
            CTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCC                                                                                  >  SRR4302535.1915539/1‑94 (MQ=60)
             TGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCA                                                                                 <  SRR4302535.1968221/94‑1 (MQ=60)
             TGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCA                                                                                 >  SRR4302535.438635/1‑94 (MQ=60)
               CCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACT                                                                               >  SRR4302535.3015006/1‑94 (MQ=60)
                CGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTG                                                                              <  SRR4302535.99971/94‑1 (MQ=60)
                 GGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCTCACTGC                                                                             >  SRR4302535.1535464/1‑94 (MQ=60)
                  GTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCG                                                                            <  SRR4302535.395810/94‑1 (MQ=60)
                  GCCCAGGTCTGCTGAGTATGCAGGTTGAATTGGGGGTCGCGTGATTTGCCAAAGGCCACGCGTGTCCCTGTGGGAGGCTGGCTGGCCCCCTGCG                                                                            <  SRR4302535.662735/94‑1 (MQ=60)
                   TCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGC                                                                           >  SRR4302535.4629775/1‑94 (MQ=60)
                   TCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGC                                                                           <  SRR4302535.1030744/94‑1 (MQ=60)
                      AGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCAT                                                                        >  SRR4302535.410846/1‑94 (MQ=60)
                        GTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCC                                                                      <  SRR4302535.1341153/94‑1 (MQ=60)
                         TCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCT                                                                     <  SRR4302535.4590050/94‑1 (MQ=60)
                         TCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCT                                                                     <  SRR4302535.2810170/94‑1 (MQ=60)
                         TCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCT                                                                     <  SRR4302535.777300/94‑1 (MQ=60)
                          CTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTC                                                                    >  SRR4302535.1197955/1‑94 (MQ=60)
                          CTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTC                                                                    >  SRR4302535.3570615/1‑94 (MQ=60)
                          CTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTC                                                                    >  SRR4302535.3515265/1‑94 (MQ=60)
                          CTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTC                                                                    >  SRR4302535.824969/1‑94 (MQ=60)
                          CTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTC                                                                    >  SRR4302535.3454899/1‑94 (MQ=60)
                           TCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCT                                                                   >  SRR4302535.4117332/1‑94 (MQ=60)
                           TCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCT                                                                   <  SRR4302535.4183721/94‑1 (MQ=60)
                           TCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCT                                                                   <  SRR4302535.106172/94‑1 (MQ=60)
                           TCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCT                                                                   <  SRR4302535.2227457/94‑1 (MQ=60)
                            CCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTC                                                                  >  SRR4302535.3982467/1‑94 (MQ=60)
                             CTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCT                                                                 <  SRR4302535.2697167/94‑1 (MQ=60)
                             CTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCT                                                                 >  SRR4302535.3647693/1‑94 (MQ=60)
                             CTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCT                                                                 >  SRR4302535.1875608/1‑94 (MQ=60)
                             CTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCT                                                                 >  SRR4302535.1849598/1‑94 (MQ=60)
                             CTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCT                                                                 >  SRR4302535.4287000/1‑94 (MQ=60)
                              TGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTT                                                                >  SRR4302535.3472877/1‑94 (MQ=60)
                                AGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCCCGCGTGTCCCTTTGGGGGGCTGGCTGGCCCCCTGCGCCATCCCCTCTTTT                                                              >  SRR4302535.3335751/1‑94 (MQ=60)
                                AGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCCTCCTCTCTTTA                                                              >  SRR4302535.2488984/1‑94 (MQ=60)
                                AGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTA                                                              <  SRR4302535.3976085/94‑1 (MQ=60)
                                 GTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAT                                                             <  SRR4302535.1995545/94‑1 (MQ=60)
                                  TATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATG                                                            >  SRR4302535.3971811/1‑94 (MQ=60)
                                   ATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGT                                                           <  SRR4302535.297153/94‑1 (MQ=60)
                                    TGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTT                                                          >  SRR4302535.2534886/1‑94 (MQ=60)
                                    TGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTT                                                          <  SRR4302535.4542580/94‑1 (MQ=60)
                                     GCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTT                                                         >  SRR4302535.16214/1‑94 (MQ=60)
                                     GCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTG                                                         <  SRR4302535.1601006/94‑1 (MQ=60)
                                       AGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTT                                                       >  SRR4302535.1090062/1‑94 (MQ=60)
                                       AGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTT                                                       >  SRR4302535.1277509/1‑94 (MQ=60)
                                         GTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGGTGTTCC                                                     >  SRR4302535.3444862/1‑94 (MQ=60)
                                          TTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCA                                                    <  SRR4302535.1663537/94‑1 (MQ=60)
                                           TGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAG                                                   <  SRR4302535.618834/94‑1 (MQ=60)
                                            GAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGA                                                  >  SRR4302535.4380806/1‑94 (MQ=60)
                                             AATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGAC                                                 <  SRR4302535.428178/94‑1 (MQ=60)
                                             AATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGAC                                                 >  SRR4302535.1188122/1‑94 (MQ=60)
                                              ATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACG                                                <  SRR4302535.349077/94‑1 (MQ=60)
                                              ATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACG                                                >  SRR4302535.4516981/1‑94 (MQ=60)
                                              ATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACG                                                >  SRR4302535.728583/1‑94 (MQ=60)
                                              ATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACG                                                >  SRR4302535.3630753/1‑94 (MQ=60)
                                              ATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCCGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACG                                                <  SRR4302535.3322850/94‑1 (MQ=60)
                                                TGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTG                                              >  SRR4302535.2150905/1‑94 (MQ=60)
                                                  CTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCC                                            >  SRR4302535.1924344/1‑94 (MQ=60)
                                                  CTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCC                                            >  SRR4302535.725145/1‑94 (MQ=60)
                                                   TGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGTGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCT                                           >  SRR4302535.461431/1‑94 (MQ=60)
                                                   TGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCT                                           >  SRR4302535.2006509/1‑94 (MQ=60)
                                                   TGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCT                                           >  SRR4302535.3153020/1‑94 (MQ=60)
                                                    GTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTG                                          >  SRR4302535.3676507/1‑94 (MQ=60)
                                                       CGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAT                                       <  SRR4302535.4378257/94‑1 (MQ=60)
                                                       CGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAT                                       >  SRR4302535.3924724/1‑94 (MQ=60)
                                                       CGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAT                                       <  SRR4302535.313869/94‑1 (MQ=60)
                                                        GCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATC                                      >  SRR4302535.1990198/1‑94 (MQ=60)
                                                        GCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATC                                      >  SRR4302535.780853/1‑94 (MQ=60)
                                                         CGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCT                                     >  SRR4302535.4500492/1‑94 (MQ=60)
                                                           TGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCT                                   <  SRR4302535.959010/94‑1 (MQ=60)
                                                           TGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCT                                   <  SRR4302535.3229810/94‑1 (MQ=60)
                                                            GATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTC                                  >  SRR4302535.1013429/1‑94 (MQ=60)
                                                              TGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGC                                >  SRR4302535.4202254/1‑94 (MQ=60)
                                                               GTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCT                               <  SRR4302535.3935581/94‑1 (MQ=60)
                                                               GTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCT                               <  SRR4302535.771719/94‑1 (MQ=60)
                                                                TGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTT                              <  SRR4302535.4511364/94‑1 (MQ=60)
                                                                TGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTT                              >  SRR4302535.1085535/1‑94 (MQ=60)
                                                                 GCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTT                             <  SRR4302535.4311025/94‑1 (MQ=60)
                                                                 GCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTT                             <  SRR4302535.2614837/94‑1 (MQ=60)
                                                                  CCAAGGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTT                            <  SRR4302535.725006/94‑1 (MQ=60)
                                                                  CCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTT                            >  SRR4302535.966378/1‑94 (MQ=60)
                                                                    AAAGGCCGCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTA                          <  SRR4302535.230692/94‑1 (MQ=60)
                                                                    AAAGGCCCCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTA                          <  SRR4302535.3711185/94‑1 (MQ=60)
                                                                    AAAGGCCACGCGTGTCCCTTTGGGAGGTTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTA                          <  SRR4302535.833625/94‑1 (MQ=60)
                                                                     AAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAC                         <  SRR4302535.355539/94‑1 (MQ=60)
                                                                     AAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAC                         >  SRR4302535.1357659/1‑94 (MQ=60)
                                                                          CACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACC                    >  SRR4302535.954310/1‑94 (MQ=60)
                                                                           ACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCA                   >  SRR4302535.2719949/1‑94 (MQ=60)
                                                                            CGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCNCCAT                  >  SRR4302535.1519865/1‑94 (MQ=60)
                                                                            CGCGTGTCCCTTTGGGAGGCTGGCGGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAT                  <  SRR4302535.1224291/94‑1 (MQ=60)
                                                                              CGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTTCCTGCATCTCTCGCTTTTTACCCACCATTT                <  SRR4302535.2994480/94‑1 (MQ=60)
                                                                              CGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTT                <  SRR4302535.2637766/94‑1 (MQ=60)
                                                                              CGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTT                <  SRR4302535.4461322/94‑1 (MQ=60)
                                                                               GTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTC               >  SRR4302535.3090780/1‑94 (MQ=60)
                                                                                TGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCG              <  SRR4302535.2524256/94‑1 (MQ=60)
                                                                                  TCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCC            >  SRR4302535.3505456/1‑94 (MQ=60)
                                                                                  TCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCC            <  SRR4302535.2227017/94‑1 (MQ=60)
                                                                                   CCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCG           >  SRR4302535.2874500/1‑94 (MQ=60)
                                                                                     CTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGAT         >  SRR4302535.855985/1‑94 (MQ=60)
                                                                                       TTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGA       <  SRR4302535.668348/94‑1 (MQ=60)
                                                                                        TGGTAGGCTGGCTGCCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGAT      <  SRR4302535.4146985/94‑1 (MQ=60)
                                                                                        TGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGAT      <  SRR4302535.216002/94‑1 (MQ=60)
                                                                                         GGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA     >  SRR4302535.2367066/1‑94 (MQ=60)
                                                                                         GGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA     <  SRR4302535.1331833/94‑1 (MQ=60)
                                                                                          GGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAG    <  SRR4302535.1028775/94‑1 (MQ=60)
                                                                                          GGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAG    <  SRR4302535.1720621/94‑1 (MQ=60)
                                                                                           GAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGC   <  SRR4302535.3146687/94‑1 (MQ=60)
                                                                                            AGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  <  SRR4302535.419314/94‑1 (MQ=60)
                                                                                            AGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  >  SRR4302535.412000/1‑94 (MQ=60)
                                                                                            AGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  <  SRR4302535.1748216/94‑1 (MQ=60)
                                                                                            AGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  <  SRR4302535.2243252/94‑1 (MQ=60)
                                                                                             |                                                                                            
GAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCC  >  NC_000913/4511272‑4511457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑