Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,541,157	C→T	R159C (CGT→TGT)	fimA →	major type 1 subunit fimbrin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,541,157	0	C	T	96.2%	702.1 / 6.0	211	R159C (CGT→TGT)	fimA	major type 1 subunit fimbrin
Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (100/103); total (105/106)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

Only 100 of 222 total aligned reads displayed.

AGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG  >  NC_000913/4541067‑4541246

aGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCgtgt                                                                                         >  1:474409/1‑93 (MQ=255)

aGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCgtgt                                                                                         >  2:646577/1‑93 (MQ=255)

aGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCgtgt                                                                                         >  1:203899/1‑93 (MQ=255)

 gAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGtt                                                                                        >  2:798059/1‑93 (MQ=255)

 gAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGGTCAGAAACAACCCTGAATAACTGAACCAATACCATTCCGTTCCAGTCGTGtt                                                                                        >  1:2406360/1‑93 (MQ=255)

  aaCGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTa                                                                                       <  2:3090490/93‑1 (MQ=255)

    cGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTAtt                                                                                     <  1:1280537/93‑1 (MQ=255)

     gggTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTAttt                                                                                    <  2:3943592/93‑1 (MQ=255)

      ggTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTAtttt                                                                                   <  1:221163/93‑1 (MQ=255)

          ctgcGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCaa                                                                               >  2:2236385/1‑93 (MQ=255)

           tgcGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAAc                                                                              >  1:1222898/1‑93 (MQ=255)

            gcgcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAAcc                                                                             <  2:3458581/93‑1 (MQ=255)

             cgcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCg                                                                            >  2:2942094/1‑93 (MQ=255)

              gcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCgg                                                                           <  1:3223277/93‑1 (MQ=255)

              gcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCgg                                                                           <  1:1459904/93‑1 (MQ=255)

               cTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCggg                                                                          >  1:1175416/1‑93 (MQ=255)

                tGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCgggg                                                                         <  2:4535388/93‑1 (MQ=255)

                 gACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGc                                                                        <  2:4260893/93‑1 (MQ=255)

                 gACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGc                                                                        >  1:1845609/1‑93 (MQ=255)

                   cGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCg                                                                      <  2:1494104/93‑1 (MQ=255)

                     cTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCa                                                                    >  1:2418038/1‑93 (MQ=255)

                      tGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATGCCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCaa                                                                   <  1:1356463/93‑1 (MQ=255)

                      tGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCaa                                                                   >  2:1351045/1‑93 (MQ=255)

                      tGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCaa                                                                   >  1:876852/1‑93 (MQ=255)

                       ggATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAc                                                                  >  2:2866211/1‑93 (MQ=255)

                       ggATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAc                                                                  >  2:4661178/1‑93 (MQ=255)

                        gATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAcc                                                                 >  1:3405030/1‑93 (MQ=255)

                        gATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAcc                                                                 <  1:210305/93‑1 (MQ=255)

                        gATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAAcc                                                                 <  2:2904080/93‑1 (MQ=255)

                          tGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAcccc                                                               <  1:508003/93‑1 (MQ=255)

                              gCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGt                                                           <  2:1274119/93‑1 (MQ=255)

                                gACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGtgc                                                         <  1:991204/93‑1 (MQ=255)

                                    tttAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTgctgct                                                     >  1:1823014/1‑93 (MQ=255)

                                        gTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCAGGGTGCTGTTAATg                                                 >  2:3412874/1‑93 (MQ=255)

                                         ttCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGc                                                <  1:2918980/93‑1 (MQ=255)

                                         ttCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGc                                                >  2:1079370/1‑93 (MQ=255)

                                           cAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCgg                                              >  2:96585/1‑93 (MQ=255)

                                             gAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGAt                                            >  2:564519/1‑93 (MQ=255)

                                               aacaacCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGc                                          <  2:419935/93‑1 (MQ=255)

                                               aacaacCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGc                                          <  1:1674909/93‑1 (MQ=255)

                                                acaacCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCg                                         >  2:211873/1‑93 (MQ=255)

                                                  aacCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAc                                       >  1:168687/1‑93 (MQ=255)

                                                   acCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAcc                                      <  1:2805865/93‑1 (MQ=255)

                                                   acCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAcc                                      >  2:1604807/1‑93 (MQ=255)

                                                     ccTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCtt                                    >  1:3004859/1‑93 (MQ=255)

                                                      cTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGTTACCGGGGCCGCCACCCCGGGTGCTGCTAATGCGGATGCGACCGTc                                   >  2:2441130/1‑93 (MQ=255)

                                                      cTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCtta                                   >  2:666817/1‑92 (MQ=255)

                                                      cTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTc                                   <  1:2951662/93‑1 (MQ=255)

                                                       tGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCa                                  <  1:3195187/93‑1 (MQ=255)

                                                       tGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCa                                  >  1:3731055/1‑93 (MQ=255)

                                                       tGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGCGCTGCTAATGCGGATGCGACCTTCa                                  >  2:3688796/1‑93 (MQ=255)

                                                        gAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCaa                                 >  2:2485177/1‑93 (MQ=255)

                                                        gAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCaa                                 <  1:1511798/93‑1 (MQ=255)

                                                        gAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCaa                                 <  1:2529009/93‑1 (MQ=255)

                                                         aaTAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAg                                <  2:1558963/93‑1 (MQ=255)

                                                         aaTAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAg                                <  1:4605676/93‑1 (MQ=255)

                                                          aTAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAgg                               <  1:4354056/93‑1 (MQ=255)

                                                           tAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGt                              >  1:1602326/1‑93 (MQ=255)

                                                           tAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGt                              <  2:1152996/93‑1 (MQ=255)

                                                           tAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCAGTGCCGCAACCCCGTGGGCTGCTAATGCGGATGCGAGCTTCCAGGt                              >  1:58253/1‑93 (MQ=255)

                                                             aCGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTc                            <  2:2255302/93‑1 (MQ=255)

                                                              cGGAACCAATACCATGCCGTTCCAGGCGTGGTAGTTTGCAACCGGGGCCGCAGCCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCa                           <  2:4118170/93‑1 (MQ=255)

                                                               ggAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAg                          <  1:3521922/93‑1 (MQ=255)

                                                                gAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGt                         >  1:1404387/1‑93 (MQ=255)

                                                                 aaCCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTa                        <  2:477662/93‑1 (MQ=255)

                                                                 aaCCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTa                        <  1:1122288/93‑1 (MQ=255)

                                                                  aCCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAt                       >  1:4192616/1‑93 (MQ=255)

                                                                   ccAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATc                      <  2:872026/93‑1 (MQ=255)

                                                                   ccAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATc                      >  2:4367019/1‑93 (MQ=255)

                                                                   ccAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATc                      >  1:4155655/1‑93 (MQ=255)

                                                                    cAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCa                     <  2:1844754/93‑1 (MQ=255)

                                                                    cAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCa                     >  2:4067551/1‑93 (MQ=255)

                                                                     aaTACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCaa                    <  1:3561342/93‑1 (MQ=255)

                                                                     aaTACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCaa                    >  1:4115385/1‑93 (MQ=255)

                                                                      aTACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAAt                   >  1:1011903/1‑93 (MQ=255)

                                                                        aCCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATaa                 <  1:1588415/93‑1 (MQ=255)

                                                                        aCCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATaa                 <  2:504781/93‑1 (MQ=255)

                                                                         ccATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAc                >  2:2062065/1‑93 (MQ=255)

                                                                         ccATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGAATCAATAAc                >  1:3988968/1‑93 (MQ=255)

                                                                            ttCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCtt             <  1:4424432/93‑2 (MQ=255)

                                                                            ttCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTa             <  1:4073810/93‑1 (MQ=255)

                                                                            ttCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTa             >  1:4204871/1‑93 (MQ=255)

                                                                            ttCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTa             >  1:3891982/1‑93 (MQ=255)

                                                                             tCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCACCCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAc            <  2:4004479/93‑1 (MQ=255)

                                                                             tCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAc            <  2:515088/93‑1 (MQ=255)

                                                                             tCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAc            <  2:4547578/93‑1 (MQ=255)

                                                                               cGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAccc          <  2:2022985/93‑1 (MQ=255)

                                                                                gTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCa         >  1:4286857/1‑93 (MQ=255)

                                                                                gTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCa         <  1:2649775/93‑1 (MQ=255)

                                                                                gTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCa         >  1:1452369/1‑93 (MQ=255)

                                                                                  tCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAgg       <  1:556754/93‑1 (MQ=255)

                                                                                    cAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGtt     <  2:2670810/93‑1 (MQ=255)

                                                                                     aGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTc    >  1:2743315/1‑93 (MQ=255)

                                                                                     aGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTc    >  2:2777228/1‑93 (MQ=255)

                                                                                     aGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTc    >  2:878589/1‑93 (MQ=255)

                                                                                      ggCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCa   >  2:2956038/1‑93 (MQ=255)

                                                                                      ggCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCa   >  1:2473804/1‑93 (MQ=255)

                                                                                      ggCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCa   <  2:3841737/93‑1 (MQ=255)

                                                                                       gcgtGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAg  >  1:3941908/1‑93 (MQ=255)

                                                                                       gcgtGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAg  >  1:3069243/1‑93 (MQ=255)

AGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG  >  NC_000913/4541067‑4541246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  NC_000913/4541066‑4541250

CAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGT                                                                                             >  SRR4302535.477733/1‑94 (MQ=60)

CAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGT                                                                                             >  SRR4302535.205295/1‑94 (MQ=60)

 AGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTT                                                                                            >  SRR4302535.2961797/1‑94 (MQ=60)

 AGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGGTCAGAAACAACCCTGAATAACTGAACCAATACCATTCCGTTCCAGTCGTGTT                                                                                            >  SRR4302535.2423683/1‑94 (MQ=60)

  GAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTA                                                                                           >  SRR4302535.1465493/1‑94 (MQ=60)

   AACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTAT                                                                                          <  SRR4302535.565332/94‑1 (MQ=60)

     CGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTT                                                                                        <  SRR4302535.1289678/94‑1 (MQ=60)

     CGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTT                                                                                        <  SRR4302535.1981101/94‑1 (MQ=60)

     CGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTT                                                                                        >  SRR4302535.2790757/1‑94 (MQ=60)

       GGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTG                                                                                      <  SRR4302535.222688/94‑1 (MQ=60)

       GGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTG                                                                                      <  SRR4302535.366134/94‑1 (MQ=60)

       GGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTG                                                                                      <  SRR4302535.4021613/94‑1 (MQ=60)

        GTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGC                                                                                     >  SRR4302535.4149849/1‑94 (MQ=60)

         TGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCA                                                                                    >  SRR4302535.1442670/1‑94 (MQ=60)

         TGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCA                                                                                    >  SRR4302535.897035/1‑94 (MQ=60)

           CTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAAC                                                                                  >  SRR4302535.1231650/1‑94 (MQ=60)

             GCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCG                                                                                <  SRR4302535.2656107/94‑1 (MQ=60)

               GCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGG                                                                              <  SRR4302535.493449/94‑1 (MQ=60)

               GCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGG                                                                              >  SRR4302535.1183807/1‑94 (MQ=60)

               GCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGG                                                                              <  SRR4302535.3245620/94‑1 (MQ=60)

               GCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGG                                                                              <  SRR4302535.1470363/94‑1 (MQ=60)

                CTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGG                                                                             >  SRR4302535.396614/1‑94 (MQ=60)

                 TGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGC                                                                            >  SRR4302535.1858909/1‑94 (MQ=60)

                  GACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCC                                                                           <  SRR4302535.1188155/94‑1 (MQ=60)

                   ACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCG                                                                          <  SRR4302535.1858023/94‑1 (MQ=60)

                    CGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGC                                                                         >  SRR4302535.3141551/1‑94 (MQ=60)

                    CGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGC                                                                         >  SRR4302535.134354/1‑94 (MQ=60)

                     GCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCA                                                                        >  SRR4302535.1714307/1‑94 (MQ=60)

                     GCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCA                                                                        >  SRR4302535.2435444/1‑94 (MQ=60)

                      CTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAA                                                                       >  SRR4302535.883061/1‑94 (MQ=60)

                      CTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAA                                                                       >  SRR4302535.1567020/1‑94 (MQ=60)

                       TGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATGCCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAAC                                                                      <  SRR4302535.1366170/94‑1 (MQ=60)

                        GGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACC                                                                     <  SRR4302535.2514227/94‑1 (MQ=60)

                        GGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACC                                                                     >  SRR4302535.3428725/1‑94 (MQ=60)

                         GATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCC                                                                    <  SRR4302535.211749/94‑1 (MQ=60)

                           TGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCG                                                                  <  SRR4302535.511555/94‑1 (MQ=60)

                           TGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCG                                                                  >  SRR4302535.1565783/1‑94 (MQ=60)

                               GCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTG                                                              <  SRR4302535.2473299/94‑1 (MQ=60)

                                CGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGC                                                             >  SRR4302535.4396350/1‑94 (MQ=60)

                                CGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGC                                                             >  SRR4302535.3420104/1‑94 (MQ=60)

                                 GACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCT                                                            <  SRR4302535.998237/94‑1 (MQ=60)

                                 GACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCT                                                            >  SRR4302535.2350518/1‑94 (MQ=60)

                                 GACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCT                                                            <  SRR4302535.3582108/94‑1 (MQ=60)

                                  ACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTG                                                           <  SRR4302535.3047082/94‑1 (MQ=60)

                                  ACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTG                                                           >  SRR4302535.4126667/1‑94 (MQ=60)

                                    ATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCT                                                         >  SRR4302535.1836181/1‑94 (MQ=60)

                                       TAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAAT                                                      <  SRR4302535.161282/94‑1 (MQ=60)

                                          TTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCG                                                   <  SRR4302535.2939579/94‑1 (MQ=60)

                                           TCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                                  >  SRR4302535.4516491/1‑94 (MQ=60)

                                            CAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGA                                                 >  SRR4302535.3934131/1‑94 (MQ=60)

                                            CAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGA                                                 <  SRR4302535.3228135/94‑1 (MQ=60)

                                               AAACAACCCTGAATAACGGACCCAATCCCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGC                                              <  SRR4302535.1288614/94‑1 (MQ=60)

                                                AACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCG                                             <  SRR4302535.1687036/94‑1 (MQ=60)

                                                AACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCG                                             >  SRR4302535.2064981/1‑94 (MQ=60)

                                                AACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCG                                             <  SRR4302535.512847/94‑1 (MQ=60)

                                                  CAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAC                                           >  SRR4302535.169860/1‑94 (MQ=60)

                                                   AACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAAgacgtacgcgagc                                          >  SRR4302535.305627/1‑81 (MQ=60)

                                                   AACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACC                                          >  SRR4302535.2885115/1‑94 (MQ=60)

                                                    ACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                         >  SRR4302535.2291979/1‑94 (MQ=60)

                                                    ACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                         <  SRR4302535.2825813/94‑1 (MQ=60)

                                                    ACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                         >  SRR4302535.114633/1‑94 (MQ=60)

                                                     CCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        >  SRR4302535.1696651/1‑94 (MQ=60)

                                                     CCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        <  SRR4302535.4429379/94‑1 (MQ=60)

                                                     CCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        >  SRR4302535.3025919/1‑94 (MQ=60)

                                                       CTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                      <  SRR4302535.2972398/94‑1 (MQ=60)

                                                       CTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                      >  SRR4302535.3756891/1‑94 (MQ=60)

                                                       CTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                      >  SRR4302535.2433087/1‑94 (MQ=60)

                                                        TGAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAA                                     <  SRR4302535.3217297/94‑1 (MQ=60)

                                                         GAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    <  SRR4302535.297793/94‑1 (MQ=60)

                                                         GAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    <  SRR4302535.1522687/94‑1 (MQ=60)

                                                         GAATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    <  SRR4302535.2547231/94‑1 (MQ=60)

                                                          AATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                                   <  SRR4302535.2699320/94‑1 (MQ=60)

                                                          AATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                                   <  SRR4302535.4636981/94‑1 (MQ=60)

                                                          AATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                                   >  SRR4302535.4233797/1‑94 (MQ=60)

                                                          AATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                                   <  SRR4302535.220951/94‑1 (MQ=60)

                                                           ATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGT                                  <  SRR4302535.4383855/94‑1 (MQ=60)

                                                           ATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGT                                  >  SRR4302535.1613886/1‑94 (MQ=60)

                                                           ATAACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCAGTGCCGCAACCCCGTGGGCTGCTAATGCGGATGCGAGCTTCCAGGT                                  >  SRR4302535.58680/1‑94 (MQ=60)

                                                           ATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGT                                  <  SRR4302535.1379519/94‑1 (MQ=60)

                                                              ACGGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCA                               <  SRR4302535.2990265/94‑1 (MQ=60)

                                                                GGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                             <  SRR4302535.3546383/94‑1 (MQ=60)

                                                                GGAACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                             >  SRR4302535.1414404/1‑94 (MQ=60)

                                                                  AACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCACCCGGCGGCGCAACCCCCAGTGCTGGTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           >  SRR4302535.4495614/1‑94 (MQ=60)

                                                                  AACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           <  SRR4302535.380205/94‑1 (MQ=60)

                                                                  AACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           <  SRR4302535.1130295/94‑1 (MQ=60)

                                                                  AACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           >  SRR4302535.4221306/1‑94 (MQ=60)

                                                                   ACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                          >  SRR4302535.4223064/1‑94 (MQ=60)

                                                                   ACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                          >  SRR4302535.4001904/1‑94 (MQ=60)

                                                                   ACCAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                          >  SRR4302535.4184103/1‑94 (MQ=60)

                                                                     CAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAA                        >  SRR4302535.523537/1‑94 (MQ=60)

                                                                     CAATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAA                        >  SRR4302535.4143555/1‑94 (MQ=60)

                                                                      AATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAAT                       <  SRR4302535.3586038/94‑1 (MQ=60)

                                                                      AATACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAAT                       >  SRR4302535.1019094/1‑94 (MQ=60)

                                                                         ACCATTCCGTTCCTGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    >  SRR4302535.2549432/1‑94 (MQ=60)

                                                                         ACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    <  SRR4302535.1599880/94‑1 (MQ=60)

                                                                         ACCATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGAATCAATAAC                    >  SRR4302535.4016249/1‑94 (MQ=60)

                                                                         ACCATGCCGTTCCAGGCGTGTTATTTTGCAACCGGTGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    <  SRR4302535.1364980/94‑1 (MQ=60)

                                                                           CATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                  <  SRR4302535.1024205/94‑1 (MQ=60)

                                                                            ATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                 >  SRR4302535.4233649/1‑94 (MQ=60)

                                                                            ATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                 >  SRR4302535.3918666/1‑94 (MQ=60)

                                                                            ATTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                 >  SRR4302535.4689052/1‑94 (MQ=60)

                                                                             TTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTTC                <  SRR4302535.4454734/94‑1 (MQ=60)

                                                                             TTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAC                <  SRR4302535.4101685/94‑1 (MQ=60)

                                                                             TTCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAC                <  SRR4302535.174586/94‑1 (MQ=60)

                                                                              TCCGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTCCC               <  SRR4302535.3504236/94‑1 (MQ=60)

                                                                                CGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA             >  SRR4302535.4316181/1‑94 (MQ=60)

                                                                                CGTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA             >  SRR4302535.1462760/1‑94 (MQ=60)

                                                                                 GTTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAG            <  SRR4302535.2668749/94‑1 (MQ=60)

                                                                                  TTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGG           <  SRR4302535.1675763/94‑1 (MQ=60)

                                                                                  TTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGG           <  SRR4302535.1500188/94‑1 (MQ=60)

                                                                                  TTCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGG           <  SRR4302535.330718/94‑1 (MQ=60)

                                                                                   TCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGT          <  SRR4302535.4201544/94‑1 (MQ=60)

                                                                                   TCCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGT          <  SRR4302535.560656/94‑1 (MQ=60)

                                                                                    CCAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT         <  SRR4302535.415486/94‑1 (MQ=60)

                                                                                     CAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC        >  SRR4302535.812313/1‑94 (MQ=60)

                                                                                     CAGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC        >  SRR4302535.2762868/1‑94 (MQ=60)

                                                                                      AGGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA       >  SRR4302535.2491647/1‑94 (MQ=60)

                                                                                       GGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG      >  SRR4302535.3090680/1‑94 (MQ=60)

                                                                                       GGCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG      >  SRR4302535.3968815/1‑94 (MQ=60)

                                                                                        GCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGG     >  SRR4302535.2696220/1‑94 (MQ=60)

                                                                                        GCGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGG     <  SRR4302535.4355250/94‑1 (MQ=60)

                                                                                         CGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    >  SRR4302535.4512212/1‑94 (MQ=60)

                                                                                         CGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  SRR4302535.2478690/94‑1 (MQ=60)

                                                                                         CGTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  SRR4302535.3096161/94‑1 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   >  SRR4302535.1582934/1‑94 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   >  SRR4302535.2394799/1‑94 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   >  SRR4302535.2370576/1‑94 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   <  SRR4302535.1597544/94‑1 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   >  SRR4302535.4497009/1‑94 (MQ=60)

                                                                                          GTGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   >  SRR4302535.4534796/1‑94 (MQ=60)

                                                                                           TGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  <  SRR4302535.3973438/94‑1 (MQ=60)

CAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  NC_000913/4541066‑4541250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`