Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,551,614 T→C L382P (CTG→CCG)  uxuB → D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,6140TC100.0% 542.2 / NA 173L382P (CTG→CCG) uxuBD‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/73);  total (100/73)

Only 100 of 191 total aligned reads displayed.

CGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGG  >  NC_000913/4551524‑4551703
                                                                                          |                                                                                         
cGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgc                                                                                         >  1:1271085/1‑93 (MQ=255)
 gctgctGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgct                                                                                        <  2:2478863/93‑1 (MQ=255)
 gctgctGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgct                                                                                        >  2:3377643/1‑93 (MQ=255)
  ctgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgctg                                                                                       >  1:4330053/1‑93 (MQ=255)
   tgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTgg                                                                                      >  2:1642551/1‑93 (MQ=255)
   tgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTgg                                                                                      >  2:4466789/1‑93 (MQ=255)
   tgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTgg                                                                                      <  2:4119838/93‑1 (MQ=255)
    gctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGa                                                                                     <  1:3791515/93‑1 (MQ=255)
     ctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCGCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAc                                                                                    <  1:527806/93‑1 (MQ=255)
     ctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAc                                                                                    >  1:4214934/1‑93 (MQ=255)
         tCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCgg                                                                                <  2:3196871/93‑1 (MQ=255)
         tCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCgg                                                                                >  1:1856308/1‑93 (MQ=255)
           gAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTg                                                                              >  1:3658035/1‑93 (MQ=255)
           cggCGTTTGAGCACCGCGTCTCTGGGTCCCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTg                                                                              <  2:579745/91‑1 (MQ=37)
            aGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGc                                                                             >  1:2737112/1‑93 (MQ=255)
             gCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCg                                                                            >  2:2122705/1‑93 (MQ=255)
               gTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTc                                                                          <  2:1023628/93‑1 (MQ=255)
               gTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTc                                                                          >  1:2386112/1‑93 (MQ=255)
                tttCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCt                                                                         >  1:2346424/1‑93 (MQ=255)
                tttCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCt                                                                         >  1:1832553/1‑93 (MQ=255)
                  tCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGc                                                                       <  1:175800/93‑1 (MQ=255)
                    aGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAc                                                                     >  2:1205672/1‑93 (MQ=255)
                     gCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAcc                                                                    >  2:4455061/1‑93 (MQ=255)
                     gCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAcc                                                                    >  1:657388/1‑93 (MQ=255)
                     gCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAcc                                                                    <  1:3566691/93‑1 (MQ=255)
                       aaaccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTg                                                                  >  1:414336/4‑93 (MQ=255)
                       aaCCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTg                                                                  >  1:1110957/1‑93 (MQ=255)
                        aCCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGc                                                                 >  1:4314489/1‑93 (MQ=255)
                         cccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCa                                                                >  2:2177783/1‑93 (MQ=255)
                          ccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaa                                                               >  1:1909552/1‑93 (MQ=255)
                            gTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaaa                                                             >  2:4674344/1‑93 (MQ=255)
                            gTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaaa                                                             <  1:893226/93‑1 (MQ=255)
                            gTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaaa                                                             >  1:2020595/1‑93 (MQ=255)
                             tctcTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAc                                                            <  1:2224398/93‑1 (MQ=255)
                               tctGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcgg                                                          >  2:1390367/1‑93 (MQ=255)
                                ctGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggc                                                         <  2:3101222/93‑1 (MQ=255)
                                  gCGTCACCGTACCTGGCAGATGGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggcgg                                                       <  2:302815/93‑1 (MQ=255)
                                   cGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACggcggc                                                      >  2:3855193/1‑93 (MQ=255)
                                    gTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCa                                                     >  1:4417560/1‑93 (MQ=255)
                                      cACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGc                                                   <  2:1993188/93‑1 (MQ=255)
                                      cACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGc                                                   <  2:2256431/93‑1 (MQ=255)
                                       cccGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCt                                                  <  1:1032873/92‑1 (MQ=255)
                                       cccGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCt                                                  <  2:194006/92‑1 (MQ=255)
                                       aCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCt                                                  >  1:1602455/1‑93 (MQ=255)
                                       aCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCt                                                  >  1:2945112/1‑93 (MQ=255)
                                        ccGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTg                                                 >  1:3746622/1‑93 (MQ=255)
                                          gTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGc                                               >  1:4464983/1‑93 (MQ=255)
                                          gTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGc                                               >  1:1073023/1‑93 (MQ=255)
                                           tACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCg                                              <  1:2462801/93‑1 (MQ=255)
                                            aCCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGt                                             >  1:2615693/1‑93 (MQ=255)
                                             ccTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTc                                            <  2:1450479/93‑1 (MQ=255)
                                              cTGGCAGATTGCGATGGACGGCAGCGAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCa                                           <  2:2930136/93‑1 (MQ=255)
                                              cTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCa                                           >  1:3469100/1‑93 (MQ=255)
                                                ggCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCACAACGGCGGCAGCTGGCGTCAcc                                         >  1:2175288/1‑93 (MQ=255)
                                                ggCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAcc                                         <  2:3445440/93‑1 (MQ=255)
                                                ggCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAcc                                         <  2:4244470/93‑1 (MQ=255)
                                                 gCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCt                                        <  2:3572138/93‑1 (MQ=255)
                                                 gCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCt                                        >  1:3372215/1‑93 (MQ=255)
                                                  cAGATTGCGATGGAGGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTg                                       <  2:930143/93‑1 (MQ=255)
                                                  cAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTg                                       <  2:808830/93‑1 (MQ=255)
                                                   aGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTgg                                      >  1:2090609/1‑93 (MQ=255)
                                                   aGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTgg                                      >  1:2311276/1‑93 (MQ=255)
                                                    gATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgc                                     >  2:3182812/1‑93 (MQ=255)
                                                    gATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgc                                     >  1:4088534/1‑93 (MQ=255)
                                                    gATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgc                                     >  1:4063278/1‑93 (MQ=255)
                                                      ttGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTGACCTGgcgc                                   >  2:1747997/1‑93 (MQ=255)
                                                      ttGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcgc                                   <  1:4606413/93‑1 (MQ=255)
                                                      ttGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcgc                                   <  1:1660043/93‑1 (MQ=255)
                                                        gCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTg                                 >  2:3829207/1‑93 (MQ=255)
                                                        gCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTg                                 <  2:2262305/93‑1 (MQ=255)
                                                         ggATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTgg                                <  1:1186152/92‑1 (MQ=255)
                                                          gATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTggg                               >  2:2807014/1‑93 (MQ=255)
                                                          gATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTggg                               <  2:3928174/93‑1 (MQ=255)
                                                          gATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCAGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTggg                               <  1:3295223/93‑1 (MQ=255)
                                                            tGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCg                             >  2:732173/1‑93 (MQ=255)
                                                              gACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtg                           <  2:2492163/93‑1 (MQ=255)
                                                                cGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAACCGGCTGCAGCTGCCGTCACCTGGCCCTGGGCGtggc                         <  2:4344067/93‑1 (MQ=255)
                                                                cGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggc                         <  1:2036841/93‑1 (MQ=255)
                                                                cGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggc                         >  2:4373226/1‑93 (MQ=255)
                                                                cGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggc                         <  2:1309610/93‑1 (MQ=255)
                                                                 ggCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggct                        >  1:3134367/1‑93 (MQ=255)
                                                                   cAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggctgg                      <  1:3416540/93‑1 (MQ=255)
                                                                    aGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggctggc                     >  1:770231/1‑93 (MQ=255)
                                                                      ccAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAACTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGgctgggtg                   >  2:4065810/1‑93 (MQ=255)
                                                                       cAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGctggctgg                  >  2:2863785/1‑93 (MQ=255)
                                                                       cAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGctggctgg                  >  1:2097704/1‑93 (MQ=255)
                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                >  1:1508788/1‑93 (MQ=255)
                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                <  2:1900651/93‑1 (MQ=255)
                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                >  1:4031233/1‑93 (MQ=255)
                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                <  2:1156382/93‑1 (MQ=255)
                                                                            gTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCg             <  2:4284643/93‑1 (MQ=255)
                                                                               tccGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTa          <  1:1045414/92‑1 (MQ=255)
                                                                               aCCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTa          <  2:1695979/93‑1 (MQ=255)
                                                                                 cGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTaca        <  1:3598850/93‑1 (MQ=255)
                                                                                  gCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTacac       <  1:2586173/93‑1 (MQ=255)
                                                                                   cAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACAcc      >  1:1489979/1‑93 (MQ=255)
                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCa    >  2:377615/1‑93 (MQ=255)
                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCa    >  2:2970778/1‑93 (MQ=255)
                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCa    >  2:3753113/1‑93 (MQ=255)
                                                                                      cGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAg   <  2:2732220/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGG  >  NC_000913/4551524‑4551703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGGCGT  >  NC_000913/4551522‑4551707
                                                                                            |                                                                                             
GACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCG                                                                                              <  SRR4302535.4118472/94‑1 (MQ=60)
 ACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGC                                                                                             >  SRR4302535.1785445/1‑94 (MQ=60)
 ACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGC                                                                                             >  SRR4302535.3273157/1‑94 (MQ=60)
 ACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGC                                                                                             >  SRR4302535.1280168/1‑94 (MQ=60)
  CGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGTTTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCT                                                                                            <  SRR4302535.3408932/94‑1 (MQ=60)
   GCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTG                                                                                           >  SRR4302535.1731309/1‑94 (MQ=60)
   GCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTG                                                                                           >  SRR4302535.4359667/1‑94 (MQ=60)
      GCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAC                                                                                        >  SRR4302535.4243780/1‑94 (MQ=60)
      GCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAC                                                                                        <  SRR4302535.3817656/94‑1 (MQ=60)
       CTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCGCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACC                                                                                       <  SRR4302535.531491/94‑1 (MQ=60)
          ATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGG                                                                                    >  SRR4302535.1869678/1‑94 (MQ=60)
            CGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTG                                                                                  >  SRR4302535.1475245/1‑94 (MQ=60)
            CGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTG                                                                                  >  SRR4302535.522157/1‑94 (MQ=60)
            CGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTG                                                                                  >  SRR4302535.2368919/1‑94 (MQ=60)
            CGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTG                                                                                  >  SRR4302535.3683357/1‑94 (MQ=60)
             GAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGC                                                                                 >  SRR4302535.2756635/1‑94 (MQ=60)
                CGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTC                                                                              >  SRR4302535.2403259/1‑94 (MQ=60)
                CGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTC                                                                              >  SRR4302535.751546/1‑94 (MQ=60)
                CGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTC                                                                              >  SRR4302535.4353508/1‑94 (MQ=60)
                 GTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCT                                                                             >  SRR4302535.1108550/1‑94 (MQ=60)
                 GTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCT                                                                             >  SRR4302535.2363241/1‑94 (MQ=60)
                 GTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCT                                                                             >  SRR4302535.1845777/1‑94 (MQ=60)
                   TTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGCCTGC                                                                           >  SRR4302535.845327/1‑94 (MQ=60)
                    TCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCA                                                                          <  SRR4302535.177021/94‑1 (MQ=60)
                      AGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACC                                                                        >  SRR4302535.661974/1‑94 (MQ=60)
                       GCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCT                                                                       <  SRR4302535.3591417/94‑1 (MQ=60)
                        CAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTG                                                                      >  SRR4302535.1118885/1‑94 (MQ=60)
                        CAAACCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTG                                                                      >  SRR4302535.417244/1‑94 (MQ=60)
                         AACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGC                                                                     >  SRR4302535.4344003/1‑94 (MQ=60)
                          ACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCA                                                                    >  SRR4302535.1902583/1‑94 (MQ=60)
                           CCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAA                                                                   >  SRR4302535.1923317/1‑94 (MQ=60)
                             CGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAA                                                                 >  SRR4302535.2035163/1‑94 (MQ=60)
                              GTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAC                                                                >  SRR4302535.4107705/1‑94 (MQ=60)
                              GTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAC                                                                <  SRR4302535.899555/94‑1 (MQ=60)
                               TCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACG                                                               <  SRR4302535.2240230/94‑1 (MQ=60)
                                CTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGG                                                              >  SRR4302535.1606904/1‑94 (MQ=60)
                                    GCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGC                                                          <  SRR4302535.1067316/94‑1 (MQ=60)
                                     CGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCA                                                         <  SRR4302535.934916/94‑1 (MQ=60)
                                     CGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCA                                                         >  SRR4302535.41989/1‑94 (MQ=60)
                                     CGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCA                                                         >  SRR4302535.4447804/1‑94 (MQ=60)
                                      GTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAG                                                        >  SRR4302535.4129059/1‑94 (MQ=60)
                                      GTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAG                                                        >  SRR4302535.593575/1‑94 (MQ=60)
                                      GTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAG                                                        >  SRR4302535.2708204/1‑94 (MQ=60)
                                        CACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCT                                                      >  SRR4302535.3383454/1‑94 (MQ=60)
                                        CACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCT                                                      >  SRR4302535.2965812/1‑94 (MQ=60)
                                        CACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCT                                                      <  SRR4302535.286688/94‑1 (MQ=60)
                                        CACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCT                                                      >  SRR4302535.1614016/1‑94 (MQ=60)
                                        CACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCT                                                      <  SRR4302535.2110985/94‑1 (MQ=60)
                                         CCCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTG                                                     <  SRR4302535.1040212/94‑1 (MQ=60)
                                         ACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTG                                                     >  SRR4302535.3772540/1‑94 (MQ=60)
                                          CCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGG                                                    >  SRR4302535.165868/1‑94 (MQ=60)
                                          CCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGG                                                    >  SRR4302535.1262903/1‑94 (MQ=60)
                                           CGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGC                                                   >  SRR4302535.1080651/1‑94 (MQ=60)
                                           CGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGC                                                   >  SRR4302535.4495606/1‑94 (MQ=60)
                                           CGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGC                                                   >  SRR4302535.2241848/1‑94 (MQ=60)
                                            GTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCG                                                  >  SRR4302535.373061/1‑94 (MQ=60)
                                             TACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGT                                                 >  SRR4302535.2634450/1‑94 (MQ=60)
                                             TACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGT                                                 <  SRR4302535.2480557/94‑1 (MQ=60)
                                               CCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCA                                               >  SRR4302535.3493207/1‑94 (MQ=60)
                                                 TGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCACAACGGCGGCAGCTGGCGTCACC                                             >  SRR4302535.2190841/1‑94 (MQ=60)
                                                  GGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCT                                            >  SRR4302535.3395673/1‑94 (MQ=60)
                                                  GGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCT                                            <  SRR4302535.2048291/94‑1 (MQ=60)
                                                   GCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTG                                           >  SRR4302535.3809094/1‑94 (MQ=60)
                                                   GCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTG                                           <  SRR4302535.2355442/94‑1 (MQ=60)
                                                    CAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGG                                          >  SRR4302535.2105674/1‑94 (MQ=60)
                                                    CAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGG                                          <  SRR4302535.2615506/94‑1 (MQ=60)
                                                    CAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGG                                          >  SRR4302535.2327815/1‑94 (MQ=60)
                                                     AGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGC                                         >  SRR4302535.4116510/1‑94 (MQ=60)
                                                     AGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGC                                         >  SRR4302535.4091086/1‑94 (MQ=60)
                                                        TTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCT                                      <  SRR4302535.4485063/94‑1 (MQ=60)
                                                        TTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCT                                      <  SRR4302535.1672041/94‑1 (MQ=60)
                                                        TTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCT                                      <  SRR4302535.4637719/94‑1 (MQ=60)
                                                          GCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCCCCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGG                                    <  SRR4302535.1507700/94‑1 (MQ=60)
                                                          GCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGG                                    >  SRR4302535.1152517/1‑94 (MQ=60)
                                                           GGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGG                                   <  SRR4302535.1194624/94‑1 (MQ=60)
                                                            GATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCAGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGC                                  <  SRR4302535.3318133/94‑1 (MQ=60)
                                                             ATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCG                                 <  SRR4302535.3479359/94‑1 (MQ=60)
                                                              TGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTCGCGTCACCTGGCCCTGGGCGT                                >  SRR4302535.198866/1‑94 (MQ=60)
                                                               GGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTG                               <  SRR4302535.3540793/94‑1 (MQ=60)
                                                                  CGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCT                            <  SRR4302535.2051508/94‑1 (MQ=60)
                                                                  CGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCT                            >  SRR4302535.3156189/1‑94 (MQ=60)
                                                                     CAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGC                         <  SRR4302535.3440308/94‑1 (MQ=60)
                                                                     CAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGC                         >  SRR4302535.775650/1‑94 (MQ=60)
                                                                       GCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTG                       <  SRR4302535.2235063/94‑1 (MQ=60)
                                                                        CCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGG                      >  SRR4302535.2112832/1‑94 (MQ=60)
                                                                          AGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGGT                    >  SRR4302535.3665361/1‑94 (MQ=60)
                                                                          AGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGGT                    >  SRR4302535.3057/1‑94 (MQ=60)
                                                                          AGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAT                    >  SRR4302535.1519632/1‑94 (MQ=60)
                                                                          AGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAT                    >  SRR4302535.4058810/1‑94 (MQ=60)
                                                                           GAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATG                   <  SRR4302535.4073378/94‑1 (MQ=60)
                                                                             AGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGTTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCG                 <  SRR4302535.3881739/94‑1 (MQ=60)
                                                                                 TCCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTAC             <  SRR4302535.1052822/94‑1 (MQ=60)
                                                                                  CCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACA            <  SRR4302535.2597137/94‑1 (MQ=60)
                                                                                   CGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACAC           >  SRR4302535.4299984/1‑94 (MQ=60)
                                                                                   CGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACAC           <  SRR4302535.3623825/94‑1 (MQ=60)
                                                                                    GCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACC          <  SRR4302535.2604747/94‑1 (MQ=60)
                                                                                    GCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACC          >  SRR4302535.1500675/1‑94 (MQ=60)
                                                                                     CAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCC         >  SRR4302535.2067112/1‑94 (MQ=60)
                                                                                      AGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCA        >  SRR4302535.450943/1‑94 (MQ=60)
                                                                                         GTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGG     <  SRR4302535.2182717/94‑1 (MQ=60)
                                                                                          TCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGGC    <  SRR4302535.2954155/94‑1 (MQ=60)
                                                                                            CGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGGCGT  >  SRR4302535.192551/1‑94 (MQ=60)
                                                                                            |                                                                                             
GACGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGGCGT  >  NC_000913/4551522‑4551707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑