Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,555,020 A→G M280T (ATG→ACG)  yjiE ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,0200AG99.7% 1232.8 / ‑5.3 390M280T (ATG→ACG) yjiEputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (196/193);  total (197/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 399 total aligned reads displayed.

TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG  >  NC_000913/4554930‑4555109
                                                                                          |                                                                                         
tGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTc                                                                                         <  1:4676984/93‑1 (MQ=255)
tGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTc                                                                                         >  2:2838567/1‑93 (MQ=255)
ggCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTc                                                                                         <  1:2285384/92‑1 (MQ=255)
 gCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTcc                                                                                        >  1:6179769/1‑93 (MQ=255)
    aCGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGt                                                                                     <  1:1214967/93‑1 (MQ=255)
    aCGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGt                                                                                     >  1:3060585/1‑93 (MQ=255)
    aCGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGt                                                                                     >  1:608002/1‑93 (MQ=255)
     cGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTa                                                                                    <  2:2693795/93‑1 (MQ=255)
     cGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTa                                                                                    <  1:7354169/93‑1 (MQ=255)
      gCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAt                                                                                   >  2:1743171/1‑93 (MQ=255)
         ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCa                                                                                <  2:6508871/93‑1 (MQ=255)
         ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGCATTCGTCCGGTATGCa                                                                                >  1:846989/1‑93 (MQ=255)
          gagaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAt                                                                               >  2:6642432/1‑93 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              <  1:1680317/93‑1 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              >  1:6713157/1‑93 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              <  2:3550691/93‑1 (MQ=255)
              aTGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGc                                                                           >  1:7509951/1‑93 (MQ=255)
               tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                          >  2:3404829/1‑93 (MQ=255)
               tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                          >  2:2617367/1‑93 (MQ=255)
                 tCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTg                                                                        >  1:4858581/1‑93 (MQ=255)
                    gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                     >  1:359754/1‑93 (MQ=255)
                    gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                     >  1:1360457/1‑93 (MQ=255)
                     cTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTTAATc                                                                    <  2:5414863/93‑1 (MQ=255)
                     cTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATc                                                                    <  1:5480249/93‑1 (MQ=255)
                      gaaGCACAACCGCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCg                                                                   <  1:6839822/92‑1 (MQ=255)
                       aaGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCgg                                                                  >  2:7098886/1‑93 (MQ=255)
                       aaGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCgg                                                                  <  2:2963715/93‑1 (MQ=255)
                          cacaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGAt                                                               <  2:7644024/93‑1 (MQ=255)
                           acaATCTCCAGCTCGCGCAGTTCGCGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                              <  2:7402556/93‑1 (MQ=255)
                           acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                              >  2:6747504/1‑93 (MQ=255)
                           acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                              >  2:4368736/1‑93 (MQ=255)
                            caATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCa                                                             <  2:7693603/93‑1 (MQ=255)
                            caATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCa                                                             >  1:2881756/1‑93 (MQ=255)
                              aTCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCAcc                                                           >  1:7711229/1‑93 (MQ=255)
                               tctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCa                                                          >  2:7056924/1‑93 (MQ=255)
                                 tcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGt                                                        >  1:7281505/1‑93 (MQ=255)
                                  ccAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGtt                                                       <  1:7114657/93‑1 (MQ=255)
                                   cAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTc                                                      >  1:4099990/1‑93 (MQ=255)
                                    aGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCg                                                     >  1:3924952/1‑93 (MQ=255)
                                    aGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGGATTCGTCCGGTATGCATAGGCCTCAATCGGGATCACCAGTTCg                                                     >  1:3475809/1‑93 (MQ=255)
                                     gCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGt                                                    >  1:5797822/1‑93 (MQ=255)
                                     gCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGt                                                    <  2:6215854/93‑1 (MQ=255)
                                       ccgcgCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTcc                                                  <  1:3803946/92‑1 (MQ=255)
                                        cgcgCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTccc                                                 <  1:5691605/93‑1 (MQ=255)
                                        cgcgCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTccc                                                 >  2:4748164/1‑93 (MQ=255)
                                           gcAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAt                                              >  1:6916284/1‑93 (MQ=255)
                                           gcAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAt                                              >  2:7257769/1‑93 (MQ=255)
                                           gcAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAt                                              >  2:5853166/1‑93 (MQ=255)
                                             aGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTc                                            >  2:2379282/1‑93 (MQ=255)
                                              gTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCa                                           >  2:7596452/1‑93 (MQ=255)
                                                tCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGt                                         >  1:1515688/1‑93 (MQ=255)
                                                tCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGt                                         <  2:1223445/93‑1 (MQ=255)
                                                tCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGt                                         <  2:4869440/93‑1 (MQ=255)
                                                 cACGCCAGAGGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                        <  2:3727454/93‑1 (MQ=255)
                                                 cACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                        <  1:1037523/93‑1 (MQ=255)
                                                 cACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                        <  1:4583379/93‑1 (MQ=255)
                                                 cACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                        <  1:721911/93‑1 (MQ=255)
                                                  aCGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAc                                       >  2:2466190/1‑93 (MQ=255)
                                                   cGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACa                                      <  2:6342644/93‑1 (MQ=255)
                                                    gCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     <  2:513733/93‑1 (MQ=255)
                                                    gCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     >  2:5221993/1‑93 (MQ=255)
                                                    gCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     <  2:3924931/93‑1 (MQ=255)
                                                    gCCAGAAGCGTTCGGCAACGGGAGTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     <  2:4088011/93‑1 (MQ=255)
                                                      cAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACg                                   >  1:5133205/1‑93 (MQ=255)
                                                       aGAAGCGTTCGGCAACGGTATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGa                                  <  1:1388162/93‑1 (MQ=255)
                                                       aGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGa                                  >  1:5080592/1‑93 (MQ=255)
                                                        gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                 >  1:6604215/1‑93 (MQ=255)
                                                        gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                 >  1:387399/1‑93 (MQ=255)
                                                             ggtCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCGGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  2:861365/91‑1 (MQ=255)
                                                             gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  1:6737806/93‑1 (MQ=255)
                                                              ttCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCGCGATTCAGTACAACGAGCTTccc                           >  2:4285035/1‑93 (MQ=255)
                                                                cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                         <  2:312577/93‑1 (MQ=255)
                                                                cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                         <  1:2570316/93‑1 (MQ=255)
                                                                 ggCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCt                        <  1:1830222/93‑1 (MQ=255)
                                                                  gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                       >  1:5627495/1‑93 (MQ=255)
                                                                  gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                       <  1:1265068/93‑1 (MQ=255)
                                                                  gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                       >  1:2445131/1‑93 (MQ=255)
                                                                   cAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGc                      <  1:476121/93‑1 (MQ=255)
                                                                   cAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGc                      <  2:4845567/93‑1 (MQ=255)
                                                                    aaCGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCg                     >  1:5268559/1‑93 (MQ=255)
                                                                      cGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGaa                   >  2:4168999/1‑93 (MQ=255)
                                                                      cGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGaa                   <  1:2600915/93‑1 (MQ=255)
                                                                       gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                  <  1:3336600/93‑1 (MQ=255)
                                                                       gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                  >  2:6880246/1‑93 (MQ=255)
                                                                       gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                  >  1:7862829/1‑93 (MQ=255)
                                                                        ggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAtt                 >  1:7775414/1‑93 (MQ=255)
                                                                          aTTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTc               >  1:2723083/1‑93 (MQ=255)
                                                                          aTTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTc               >  1:4433479/1‑93 (MQ=255)
                                                                             cATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttg            <  1:4718818/93‑1 (MQ=255)
                                                                               tGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgtt          >  1:6412208/1‑93 (MQ=255)
                                                                               tGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgtt          >  1:6332389/1‑93 (MQ=255)
                                                                               tGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgtt          <  1:1865945/93‑1 (MQ=255)
                                                                               tGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgtt          <  2:1903699/93‑1 (MQ=255)
                                                                                gCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgttg         <  2:4605884/93‑1 (MQ=255)
                                                                                  gggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGa       <  2:5691957/93‑1 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGag      >  2:1039687/1‑92 (MQ=255)
                                                                                    gTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATg     <  2:1515688/93‑1 (MQ=255)
                                                                                    gTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATg     <  2:376892/93‑1 (MQ=255)
                                                                                      aTTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGc   <  1:2068095/93‑1 (MQ=255)
                                                                                       ttCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCg  >  2:3267790/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG  >  NC_000913/4554930‑4555109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               >  SRR4302543.1818911/1‑94 (MQ=60)
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               >  SRR4302543.2494604/1‑94 (MQ=60)
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               <  SRR4302543.4624175/94‑1 (MQ=60)
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACCCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               >  SRR4302543.5929542/1‑94 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              >  SRR4302543.2099066/1‑94 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              >  SRR4302543.3639178/1‑94 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              >  SRR4302543.1437859/1‑94 (MQ=60)
  GTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTC                                                                                             >  SRR4302543.1978231/1‑94 (MQ=60)
  GTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTC                                                                                             >  SRR4302543.4041245/1‑94 (MQ=60)
   TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCC                                                                                            >  SRR4302543.6238186/1‑94 (MQ=60)
   TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCC                                                                                            <  SRR4302543.4719623/94‑1 (MQ=60)
   GGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCC                                                                                            <  SRR4302543.2306258/94‑1 (MQ=60)
   GGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCC                                                                                            <  SRR4302543.5756595/94‑1 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302543.6003135/94‑1 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302543.736667/94‑1 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGG                                                                                          >  SRR4302543.4206679/1‑94 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGG                                                                                          >  SRR4302543.4747987/1‑94 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGG                                                                                          >  SRR4302543.950416/1‑94 (MQ=60)
      GACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGT                                                                                         >  SRR4302543.3088119/1‑94 (MQ=60)
      GACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGT                                                                                         <  SRR4302543.2561314/94‑1 (MQ=60)
      GACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGT                                                                                         >  SRR4302543.1147005/1‑94 (MQ=60)
      GACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGT                                                                                         >  SRR4302543.5685239/1‑94 (MQ=60)
       ACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTA                                                                                        <  SRR4302543.1225883/94‑1 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       <  SRR4302543.7424911/94‑1 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       >  SRR4302543.7690501/1‑94 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       <  SRR4302543.1257625/94‑1 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATTTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302543.7345472/94‑1 (MQ=60)
          CTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGC                                                                                     >  SRR4302543.4626207/1‑94 (MQ=60)
           ccGAGAATGGCAGCTAACCACAATCTCCCGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    <  SRR4302543.5694081/92‑1 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    >  SRR4302543.6459352/1‑94 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    >  SRR4302543.1963551/1‑94 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    <  SRR4302543.5694080/94‑1 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGCATTCGTCCGGTATGCA                                                                                    >  SRR4302543.854497/1‑94 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   <  SRR4302543.1881712/94‑1 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   >  SRR4302543.1723035/1‑94 (MQ=60)
             GAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATG                                                                                  >  SRR4302543.6785117/1‑94 (MQ=60)
             GAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATA                                                                                  >  SRR4302543.6776589/1‑94 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCATCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302543.6430178/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302543.1695393/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 >  SRR4302543.2258028/1‑94 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                >  SRR4302543.4362037/1‑94 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302543.1392105/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302543.1850634/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302543.7312580/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302543.3939501/94‑1 (MQ=60)
               AAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302543.4665166/94‑1 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              <  SRR4302543.5140439/94‑1 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              <  SRR4302543.6713491/94‑1 (MQ=60)
                   GTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTG                                                                            >  SRR4302543.4902883/1‑94 (MQ=60)
                    TCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGCTTCATGCGGTGATTCGTCCGGGATGCATAGGCCTGA                                                                           >  SRR4302543.7178430/1‑94 (MQ=60)
                    TCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGA                                                                           >  SRR4302543.7178429/1‑94 (MQ=60)
                    TCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGA                                                                           >  SRR4302543.6656107/1‑94 (MQ=60)
                    TCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGA                                                                           <  SRR4302543.553494/94‑1 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302543.2854781/1‑94 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302543.2486656/1‑94 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302543.6114665/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302543.1372645/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         <  SRR4302543.2797707/94‑1 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302543.1372644/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302543.7474922/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302543.363080/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         <  SRR4302543.2439908/94‑1 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302543.185450/94‑1 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302543.3375757/94‑1 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGACAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302543.6682714/94‑1 (MQ=60)
                        CTAAGTACATTCTCCAGCGCGCGCATTTCACGCCAGAAGCGTACGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       <  SRR4302543.5750756/94‑1 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       <  SRR4302543.5529918/94‑1 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       >  SRR4302543.1413975/1‑94 (MQ=60)
                         caaacacaaaccccacCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTAGTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      <  SRR4302543.6904377/78‑1 (MQ=60)
                         GAAGCACAACCGCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      <  SRR4302543.6904380/94‑1 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     <  SRR4302543.7733641/94‑1 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     >  SRR4302543.1578353/1‑94 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     <  SRR4302543.2592880/94‑1 (MQ=60)
                           AGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGA                                                                    >  SRR4302543.6159397/1‑94 (MQ=60)
                              ACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCA                                                                 >  SRR4302543.2907981/1‑94 (MQ=60)
                                AATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACC                                                               <  SRR4302543.5278289/94‑1 (MQ=60)
                                AATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACC                                                               >  SRR4302543.7784174/1‑94 (MQ=60)
                                AATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACC                                                               >  SRR4302543.7784173/1‑94 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              >  SRR4302543.7828393/1‑94 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              >  SRR4302543.6828821/1‑94 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              >  SRR4302543.3895239/1‑94 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              <  SRR4302543.3744266/94‑1 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGGATTCGTCCGGGATGCATAGGCCTGAATCGGGATCCCCA                                                              >  SRR4302543.6150126/1‑94 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            >  SRR4302543.7351822/1‑94 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            >  SRR4302543.5132347/1‑94 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            <  SRR4302543.1293120/94‑1 (MQ=60)
                                    TCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTT                                                           >  SRR4302543.6256612/1‑94 (MQ=60)
                                    TCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTT                                                           <  SRR4302543.6950841/94‑1 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          >  SRR4302543.484124/1‑94 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          <  SRR4302543.7183772/94‑1 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          <  SRR4302543.2125845/94‑1 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          >  SRR4302543.2969647/1‑94 (MQ=60)
                                      CAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCG                                                         >  SRR4302543.3959591/1‑94 (MQ=60)
                                      CAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCG                                                         <  SRR4302543.1773831/94‑1 (MQ=60)
                                      CAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGGATTCGTCCGGTATGCATAGGCCTCAATCGGGATCACCAGTTCG                                                         >  SRR4302543.3506940/1‑94 (MQ=60)
                                       AGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGT                                                        >  SRR4302543.5850562/1‑94 (MQ=60)
                                         CTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCC                                                      >  SRR4302543.4025014/1‑94 (MQ=60)
                                         CTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCC                                                      <  SRR4302543.395836/94‑1 (MQ=60)
                                          TCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCC                                                     <  SRR4302543.1505057/94‑1 (MQ=60)
                                          CCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCC                                                     <  SRR4302543.3837464/94‑1 (MQ=60)
                                           CGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCG                                                    <  SRR4302543.5743547/94‑1 (MQ=60)
                                            GCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGA                                                   <  SRR4302543.4555221/94‑1 (MQ=60)
                                             CGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAT                                                  <  SRR4302543.5449494/94‑1 (MQ=60)
                                             CGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAT                                                  >  SRR4302543.6981300/1‑94 (MQ=60)
                                              GCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATT                                                 <  SRR4302543.3857703/94‑1 (MQ=60)
                                               CAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTC                                                <  SRR4302543.7881180/94‑1 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               >  SRR4302543.7910518/1‑94 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               <  SRR4302543.4808792/94‑1 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGACTCGGGATAACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.6934801/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.2604820/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.7695359/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.1598100/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.3907029/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302543.5164517/1‑94 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302543.728426/94‑1 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302543.1046747/94‑1 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302543.4691543/94‑1 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302543.4625028/94‑1 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           >  SRR4302543.2438465/1‑94 (MQ=60)
                                                         CAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGA                                      >  SRR4302543.2689508/1‑94 (MQ=60)
                                                         CAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGA                                      >  SRR4302543.5126670/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGTATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     <  SRR4302543.1400656/94‑1 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.8005386/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.2694646/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.7297643/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.1356733/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.6666458/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302543.390984/1‑94 (MQ=60)
                                                           GAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGC                                    >  SRR4302543.2628412/1‑94 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCT                                   <  SRR4302543.1234716/94‑1 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATCCAGTACAACGAGCT                                   <  SRR4302543.5274809/94‑1 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCT                                   >  SRR4302543.5662943/1‑94 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  <  SRR4302543.6048624/94‑1 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  >  SRR4302543.745292/1‑94 (MQ=60)
                                                              GCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTC                                 >  SRR4302543.3984229/1‑94 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCCCCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302543.7218952/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               >  SRR4302543.6779505/1‑94 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302543.6801443/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302543.1974652/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302543.7218953/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               >  SRR4302543.6363925/1‑94 (MQ=60)
                                                                 TTCGGCAACGGGATTCATGCTGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCG                              <  SRR4302543.739894/94‑1 (MQ=60)
                                                                 TTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCG                              <  SRR4302543.6428149/94‑1 (MQ=60)
                                                                  TCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGC                             <  SRR4302543.4547857/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302543.840892/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302543.2593766/94‑1 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           >  SRR4302543.1454477/1‑94 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           <  SRR4302543.1846665/94‑1 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           <  SRR4302543.6139500/94‑1 (MQ=60)
                                                                     GCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGC                          <  SRR4302543.1276394/94‑1 (MQ=60)
                                                                     GCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGC                          >  SRR4302543.2973369/1‑94 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         >  SRR4302543.3571021/1‑94 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         >  SRR4302543.5316094/1‑94 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         <  SRR4302543.480522/94‑1 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         >  SRR4302543.5316093/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        <  SRR4302543.5395988/94‑1 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302543.935508/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302543.1336089/1‑94 (MQ=60)
                                                                        ACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAA                       >  SRR4302543.3578399/1‑94 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      >  SRR4302543.1598606/1‑94 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      <  SRR4302543.2624536/94‑1 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      >  SRR4302543.7936881/1‑94 (MQ=60)
                                                                          GGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATT                     >  SRR4302543.232577/1‑94 (MQ=60)
                                                                          GGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATT                     >  SRR4302543.7848744/1‑94 (MQ=60)
                                                                          GGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATT                     <  SRR4302543.3366663/94‑1 (MQ=60)
                                                                           GGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTT                    <  SRR4302543.5648604/94‑1 (MQ=60)
                                                                           GGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTT                    >  SRR4302543.747200/1‑94 (MQ=60)
                                                                            GATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTC                   >  SRR4302543.4473633/1‑94 (MQ=60)
                                                                            GATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGAGAGCACCAGGTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTC                   >  SRR4302543.4473632/1‑94 (MQ=60)
                                                                             ATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCT                  >  SRR4302543.34327/1‑94 (MQ=60)
                                                                             ATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCT                  >  SRR4302543.2749725/1‑94 (MQ=60)
                                                                              TTCATGCGGGTATTCGTCCTGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTT                 <  SRR4302543.3778092/94‑1 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                <  SRR4302543.1113143/94‑1 (MQ=60)
                                                                                tagGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302543.4761934/91‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               >  SRR4302543.3229226/1‑94 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302543.7858130/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302543.4761935/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302543.5120527/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               >  SRR4302543.5194003/1‑94 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              >  SRR4302543.6904445/1‑94 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              <  SRR4302543.4803627/94‑1 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              >  SRR4302543.6391942/1‑94 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              >  SRR4302543.6472590/1‑94 (MQ=60)
                                                                                   GCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGG            >  SRR4302543.2619763/1‑94 (MQ=60)
                                                                                    CGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGA           <  SRR4302543.4875349/94‑1 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAT          >  SRR4302543.4789109/1‑94 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATG         >  SRR4302543.4924781/1‑94 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATG         <  SRR4302543.6640352/94‑1 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        >  SRR4302543.30898/1‑94 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        >  SRR4302543.344769/1‑94 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        >  SRR4302543.6745300/1‑94 (MQ=60)
                                                                                         ATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG      <  SRR4302543.2086582/94‑1 (MQ=60)
                                                                                         ATTCGTCCGCTATGCATAGGCCTGAATCGGGATCTCCAGTTCGGCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG      <  SRR4302543.7176883/94‑1 (MQ=60)
                                                                                           TCGTCCGGTATGCATAGGTCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTA    <  SRR4302543.2776558/94‑1 (MQ=60)
                                                                                           TCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTA    <  SRR4302543.2776559/94‑1 (MQ=60)
                                                                                            CGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAC   <  SRR4302543.5850079/94‑1 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  <  SRR4302543.3101936/94‑1 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  SRR4302543.7591965/1‑94 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  SRR4302543.266514/1‑94 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCAGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  SRR4302543.5155266/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑