Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,555,020 A→G M280T (ATG→ACG)  yjiE ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,0200AG100.0% 690.7 / NA 218M280T (ATG→ACG) yjiEputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (97/121);  total (97/121)

Only 100 of 224 total aligned reads displayed.

CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGC  >  NC_000913/4554932‑4555108
                                                                                        |                                                                                        
 gACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGGTCGGCAACGGGCTTCATGCGGGTATTCGTCCgg                                                                                     >  1:122166/1‑93 (MQ=255)
 gACCCTGGAGAATGTCAGCTACGGACAATCGCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCACCGGGATTCCTGCGGGTATTCGTCCgg                                                                                     <  2:434093/93‑1 (MQ=255)
   cGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTa                                                                                   >  2:917523/1‑93 (MQ=255)
      tGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGc                                                                                >  1:3557234/1‑93 (MQ=255)
      tGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGc                                                                                >  2:1547577/1‑93 (MQ=255)
       ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGGAGCGTTCGACAACGGGATTCATGAGGGTATTCGTTCGGTATGCa                                                                               >  1:1261734/1‑93 (MQ=255)
       ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCa                                                                               >  1:3288934/1‑93 (MQ=255)
       ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCa                                                                               <  1:3657520/93‑1 (MQ=255)
        gagaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAt                                                                              >  1:2810140/1‑93 (MQ=255)
         agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                             <  2:2531536/93‑1 (MQ=255)
         agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                             <  2:1002039/93‑1 (MQ=255)
           aaTGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAgg                                                                           <  2:4083390/93‑1 (MQ=255)
           aaTGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAgg                                                                           >  2:1628734/1‑93 (MQ=255)
            aTGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGc                                                                          <  2:2169901/93‑1 (MQ=255)
             tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                         <  2:2483243/93‑1 (MQ=255)
              gTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCt                                                                        <  1:2108013/93‑1 (MQ=255)
               tCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTg                                                                       >  1:143043/1‑93 (MQ=255)
                cAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGa                                                                      <  2:2977718/93‑1 (MQ=255)
                 aGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGaa                                                                     <  2:2267808/93‑1 (MQ=255)
                  gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                    >  1:960702/1‑93 (MQ=255)
                  gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                    <  1:315057/93‑1 (MQ=255)
                   cTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATc                                                                   <  2:947008/93‑1 (MQ=255)
                    tAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCg                                                                  <  1:4528704/93‑1 (MQ=255)
                     aaGCACAATCTCCAGCTCGCGCCGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCgg                                                                 >  1:152413/1‑93 (MQ=255)
                      aGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCggg                                                                >  1:4602849/1‑93 (MQ=255)
                       gCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGa                                                               <  2:2039638/93‑1 (MQ=255)
                       gCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGa                                                               <  2:1095821/93‑1 (MQ=255)
                         acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                             >  1:1969838/1‑93 (MQ=255)
                         acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                             >  1:4122375/1‑93 (MQ=255)
                         acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                             >  2:2622370/1‑93 (MQ=255)
                          caATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCa                                                            <  1:1653647/93‑1 (MQ=255)
                           aaTCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCAc                                                           >  2:2326400/1‑93 (MQ=255)
                             tctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCa                                                         <  2:1770993/93‑1 (MQ=255)
                             tctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCa                                                         >  1:187925/1‑93 (MQ=255)
                             tctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCa                                                         >  2:837188/1‑93 (MQ=255)
                              ctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAg                                                        <  1:2010240/93‑1 (MQ=255)
                                ccAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGtt                                                      >  1:536639/1‑93 (MQ=255)
                                   gCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGt                                                   >  2:129210/1‑93 (MQ=255)
                                     tCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTcc                                                 <  2:4300362/93‑1 (MQ=255)
                                     tCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTcc                                                 >  1:2785280/1‑93 (MQ=255)
                                     tCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTcc                                                 >  2:507700/1‑93 (MQ=255)
                                        cgcAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGa                                              <  2:2218517/93‑1 (MQ=255)
                                         gcAGTTCACGCCAGAAGCGCTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACTAGTTCGTCCCGAt                                             >  2:3909205/1‑93 (MQ=255)
                                          cAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAtt                                            <  1:4328327/93‑1 (MQ=255)
                                           aGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTc                                           >  2:1598630/1‑93 (MQ=255)
                                            gTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCa                                          >  1:42084/1‑93 (MQ=255)
                                            gTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCa                                          >  2:3445092/1‑93 (MQ=255)
                                             ttCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAg                                         <  1:4289403/93‑1 (MQ=255)
                                             ttCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAg                                         <  1:3528083/93‑1 (MQ=255)
                                             ttCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAg                                         <  1:415158/93‑1 (MQ=255)
                                               cACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                       <  2:275018/93‑1 (MQ=255)
                                                aCGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAc                                      <  1:4461697/93‑1 (MQ=255)
                                                  gCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                    <  2:1601998/93‑1 (MQ=255)
                                                   ccAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAc                                   <  1:1013176/93‑1 (MQ=255)
                                                   ccAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAc                                   >  2:2099240/1‑93 (MQ=255)
                                                     aGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGa                                 <  1:1119157/93‑1 (MQ=255)
                                                      gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAGTCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                >  2:3879925/1‑93 (MQ=255)
                                                      gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                >  1:1180646/1‑93 (MQ=255)
                                                      gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                >  2:1456967/1‑93 (MQ=255)
                                                       aaGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGc                               <  2:2916220/93‑1 (MQ=255)
                                                       aaGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGc                               <  1:2531953/93‑1 (MQ=255)
                                                       aaGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGc                               <  2:3295445/93‑1 (MQ=255)
                                                        aGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                              >  2:1988492/1‑93 (MQ=255)
                                                        aGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                              >  1:3200790/1‑93 (MQ=255)
                                                        aGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                              <  1:686063/93‑1 (MQ=255)
                                                        aGCGTGCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                              <  2:415252/93‑1 (MQ=255)
                                                         gCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCtt                             >  1:350695/1‑93 (MQ=255)
                                                           gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                           >  2:4039529/1‑93 (MQ=255)
                                                           gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                           >  1:2978527/1‑93 (MQ=255)
                                                            ttCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTccc                          >  2:670862/1‑93 (MQ=255)
                                                            tgcGGCAACGGGATTCATGCTGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCCCCAGTTCGTCCCGATTCAGTACAACGAGCTTccc                          <  1:596841/91‑1 (MQ=255)
                                                              cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                        <  2:579532/93‑1 (MQ=255)
                                                               ggCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCt                       >  1:243667/1‑93 (MQ=255)
                                                                gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                      <  2:218198/93‑1 (MQ=255)
                                                                 cAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGc                     <  2:3004181/93‑1 (MQ=255)
                                                                  aaCGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCg                    >  1:3673714/1‑93 (MQ=255)
                                                                   aCGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGa                   >  2:2379166/1‑93 (MQ=255)
                                                                   aCGGGATTCATGCGGGTATTCGCCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGATCTTCCCGCTGCGa                   >  1:2981978/1‑93 (MQ=255)
                                                                   aCGGGATTCATGCGGGTATCCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGa                   >  2:4573056/1‑93 (MQ=255)
                                                                    cGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGaa                  <  2:2145419/93‑1 (MQ=255)
                                                                     gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                 <  2:3638491/93‑1 (MQ=255)
                                                                     gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                 <  1:2661060/93‑1 (MQ=255)
                                                                        aTTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTc              <  1:3769721/93‑1 (MQ=255)
                                                                         ttCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCt             <  1:8316/93‑1 (MQ=255)
                                                                           cATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCGGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttg           <  1:416841/93‑1 (MQ=255)
                                                                           cATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttg           <  2:3100462/93‑1 (MQ=255)
                                                                           cATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttg           <  2:1892698/93‑1 (MQ=255)
                                                                            aTGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgt          <  1:3834066/93‑1 (MQ=255)
                                                                             tGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgtt         <  2:854223/93‑1 (MQ=255)
                                                                              gCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgttg        <  1:902616/93‑1 (MQ=255)
                                                                                gggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGa      >  1:2185369/1‑93 (MQ=255)
                                                                                 ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAt     <  2:1187357/93‑1 (MQ=255)
                                                                                  gTATTCGTCCGGTATGCATAGGCCTGAATCGGGGTCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTAGATg    >  1:3030528/1‑93 (MQ=255)
                                                                                  gTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATg    <  2:3525429/93‑1 (MQ=255)
                                                                                  gTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATg    <  1:3429912/93‑1 (MQ=255)
                                                                                   tATTCGTCCGGTATGCATAGGGCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATgg   >  2:4664500/1‑93 (MQ=255)
                                                                                   tATTCGTCCGGTATGCATAGGCCTGAATCGGGATCCCCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATgg   <  1:73748/93‑1 (MQ=255)
                                                                                   tATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATgg   >  2:4387558/1‑93 (MQ=255)
                                                                                   tATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATgg   <  1:1635828/93‑1 (MQ=255)
                                                                                    aTTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGc  >  1:3449453/1‑93 (MQ=255)
                                                                                        |                                                                                        
CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGC  >  NC_000913/4554932‑4555108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               <  SRR4302535.1527510/94‑1 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              <  SRR4302535.2671328/94‑1 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              <  SRR4302535.3740843/94‑1 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              >  SRR4302535.1298203/1‑94 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGG                                                                                          <  SRR4302535.3716008/94‑1 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGGTCGGCAACGGGCTTCATGCGGGTATTCGTCCGG                                                                                          >  SRR4302535.123022/1‑94 (MQ=60)
       ACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTA                                                                                        <  SRR4302535.995587/94‑1 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATCTCCATCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302535.301634/94‑1 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302535.706380/94‑1 (MQ=60)
          CTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGC                                                                                     >  SRR4302535.3581907/1‑94 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGGAGCGTTCGACAACGGGATTCATGAGGGTATTCGTTCGGTATGCA                                                                                    >  SRR4302535.1270761/1‑94 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    >  SRR4302535.3311802/1‑94 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   <  SRR4302535.3682838/94‑1 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   >  SRR4302535.2830117/1‑94 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   <  SRR4302535.4205753/94‑1 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   >  SRR4302535.1656415/1‑94 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302535.3149913/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302535.2664887/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302535.994243/94‑1 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              >  SRR4302535.4330881/1‑94 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              >  SRR4302535.4633561/1‑94 (MQ=60)
                  TGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCT                                                                             >  SRR4302535.4426690/1‑94 (MQ=60)
                   GTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTG                                                                            <  SRR4302535.2123216/94‑1 (MQ=60)
                   GTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTG                                                                            <  SRR4302535.2123215/94‑1 (MQ=60)
                   GTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTG                                                                            >  SRR4302535.144040/1‑94 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          <  SRR4302535.846169/94‑1 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302535.1441319/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302535.967527/1‑94 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302535.4624931/94‑1 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302535.3472497/94‑1 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302535.317234/94‑1 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTCTGCATACGCCTGcctcg                                                                       >  SRR4302535.3405780/1‑89 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       <  SRR4302535.536337/94‑1 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       >  SRR4302535.683161/1‑94 (MQ=60)
                         TAAGCACAATCTCCAGCTCGCGCCGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      >  SRR4302535.153482/1‑94 (MQ=60)
                         TAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      <  SRR4302535.4531271/94‑1 (MQ=60)
                         TAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      <  SRR4302535.4559715/94‑1 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     >  SRR4302535.4634149/1‑94 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     >  SRR4302535.1043586/1‑94 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     <  SRR4302535.1338255/94‑1 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     <  SRR4302535.2312017/94‑1 (MQ=60)
                           AGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGA                                                                    <  SRR4302535.1235702/94‑1 (MQ=60)
                             CACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATC                                                                  >  SRR4302535.1984029/1‑94 (MQ=60)
                             CACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATC                                                                  >  SRR4302535.4150584/1‑94 (MQ=60)
                              ACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGCATCGGGATCA                                                                 >  SRR4302535.3545031/1‑94 (MQ=60)
                               CAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCAC                                                                <  SRR4302535.1665613/94‑1 (MQ=60)
                               CAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCAC                                                                <  SRR4302535.780594/94‑1 (MQ=60)
                                AATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACC                                                               >  SRR4302535.116010/1‑94 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              >  SRR4302535.189226/1‑94 (MQ=60)
                                  TCTCCAGCTCGCGCAGTTCACGGCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCATGAATCGGGATCACCAG                                                             >  SRR4302535.300088/1‑94 (MQ=60)
                                  TCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAG                                                             <  SRR4302535.197424/94‑1 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            >  SRR4302535.1588788/1‑94 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            <  SRR4302535.2024713/94‑1 (MQ=60)
                                    TCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTT                                                           >  SRR4302535.540389/1‑94 (MQ=60)
                                    TCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTT                                                           <  SRR4302535.177309/94‑1 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          <  SRR4302535.3692830/94‑1 (MQ=60)
                                       AGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGT                                                        <  SRR4302535.3372910/94‑1 (MQ=60)
                                         CTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCC                                                      <  SRR4302535.2820487/94‑1 (MQ=60)
                                         CTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCC                                                      >  SRR4302535.2805099/1‑94 (MQ=60)
                                          TCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCC                                                     >  SRR4302535.4253373/1‑94 (MQ=60)
                                            GCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGA                                                   >  SRR4302535.1214337/1‑94 (MQ=60)
                                               CAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTC                                                <  SRR4302535.4357922/94‑1 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGNAANGGGATTCATGCNGGTATTCNTCCGGNATNCATAGGCCNGAATCGNNNTNACCAGNTNGTncnnntnta                                               >  SRR4302535.1309802/1‑85 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               >  SRR4302535.42405/1‑94 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               >  SRR4302535.404653/1‑94 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               >  SRR4302535.4192329/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              >  SRR4302535.941824/1‑94 (MQ=60)
                                                  TTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGT                                             <  SRR4302535.3552584/94‑1 (MQ=60)
                                                  TTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGT                                             <  SRR4302535.418070/94‑1 (MQ=60)
                                                  TTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGT                                             <  SRR4302535.4318740/94‑1 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302535.3647290/94‑1 (MQ=60)
                                                     ACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACA                                          <  SRR4302535.4492298/94‑1 (MQ=60)
                                                       CCCAGAAGCGTTCGTCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAC                                        <  SRR4302535.3853283/94‑1 (MQ=60)
                                                        CCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACG                                       <  SRR4302535.1020382/94‑1 (MQ=60)
                                                         CAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGA                                      >  SRR4302535.3370051/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     <  SRR4302535.1127141/94‑1 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302535.1189076/1‑94 (MQ=60)
                                                           GAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCTGATTCAGTACAACGAGC                                    <  SRR4302535.3558376/94‑1 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCT                                   <  SRR4302535.2550198/94‑1 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCT                                   >  SRR4302535.3222951/1‑94 (MQ=60)
                                                            AAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCT                                   >  SRR4302535.3046047/1‑94 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  >  SRR4302535.353112/1‑94 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  <  SRR4302535.690868/94‑1 (MQ=60)
                                                               CGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCC                                >  SRR4302535.2999426/1‑94 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302535.3943870/94‑1 (MQ=60)
                                                                 TTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCG                              <  SRR4302535.3644035/94‑1 (MQ=60)
                                                                 TGCGGCAACGGGATTCATGCTGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCCCCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCC                              <  SRR4302535.601021/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            >  SRR4302535.245337/1‑94 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302535.4277754/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302535.57589/94‑1 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           >  SRR4302535.2524012/1‑94 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         <  SRR4302535.1761897/94‑1 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         >  SRR4302535.3699152/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        <  SRR4302535.201092/94‑1 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGCCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGATCTTCCCGCTGCGA                        >  SRR4302535.3002895/1‑94 (MQ=60)
                                                                        ACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGGTCAACCGTTCGTCCCGctttcgtaccaacagcttcccggtgcgga                       >  SRR4302535.352472/1‑65 (MQ=60)
                                                                          GGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATT                     <  SRR4302535.2680121/94‑1 (MQ=60)
                                                                            GATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTC                   >  SRR4302535.2727785/1‑94 (MQ=60)
                                                                             ATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCT                  <  SRR4302535.3795738/94‑1 (MQ=60)
                                                                             ATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCT                  >  SRR4302535.1385538/1‑94 (MQ=60)
                                                                             ATTCATGCGGGTATTCGTCCGGCATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCT                  >  SRR4302535.2892175/1‑94 (MQ=60)
                                                                              TTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTT                 <  SRR4302535.8385/94‑1 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                <  SRR4302535.4177172/94‑1 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                >  SRR4302535.700179/1‑94 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                <  SRR4302535.319267/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCGGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302535.419760/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               >  SRR4302535.2129329/1‑94 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              <  SRR4302535.3860504/94‑1 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              >  SRR4302535.2700300/1‑94 (MQ=60)
                                                                                   GCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGG            <  SRR4302535.909009/94‑1 (MQ=60)
                                                                                    CGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGA           >  SRR4302535.2200945/1‑94 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGGTCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTAGATG         >  SRR4302535.3051744/1‑94 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATG         <  SRR4302535.2123476/94‑1 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTTGATGG        >  SRR4302535.3253050/1‑94 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        <  SRR4302535.3453750/94‑1 (MQ=60)
                                                                                        TATTCGTCCGGTATGCATAGGCCTGAATCGGGATCCCCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGC       <  SRR4302535.74283/94‑1 (MQ=60)
                                                                                        TATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGC       >  SRR4302535.3473434/1‑94 (MQ=60)
                                                                                        TATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGC       <  SRR4302535.1647649/94‑1 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  SRR4302535.237428/1‑94 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAAT  >  SRR4302535.1305659/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑