Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,561,105	A→G	V60A (GTG→GCG)	yjiK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,561,105	0	A	G	95.7%	660.5 / 14.1	231	V60A (GTG→GCG)	yjiK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/8); new base G (71/150); total (73/158)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

Only 100 of 240 total aligned reads displayed.

ATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATC  >  NC_000913/4561015‑4561194

aTTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCt                                                                                         >  2:768438/1‑93 (MQ=255)

aTTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCt                                                                                         >  1:4322631/1‑93 (MQ=255)

aTTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCt                                                                                         <  2:4211636/93‑1 (MQ=255)

aTTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCt                                                                                         >  1:1638702/1‑93 (MQ=255)

aTTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCt                                                                                         >  2:1538584/1‑93 (MQ=255)

   tCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGcc                                                                                      >  1:2203879/1‑93 (MQ=255)

    cTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCCGCca                                                                                     >  1:2532478/1‑93 (MQ=255)

     tttGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCcac                                                                                    >  1:1345949/1‑93 (MQ=255)

       tGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACaa                                                                                  >  1:4441607/1‑93 (MQ=255)

        gCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAAt                                                                                 <  1:671807/93‑1 (MQ=255)

        gCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAAt                                                                                 <  2:295343/93‑1 (MQ=255)

           gTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGc                                                                              <  1:208564/93‑1 (MQ=255)

           gTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGc                                                                              <  1:3654768/93‑1 (MQ=255)

            tCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCa                                                                             >  2:1463140/1‑93 (MQ=255)

               aTGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCaaaa                                                                          >  1:3502069/1‑93 (MQ=255)

               aTGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCaaaa                                                                          >  1:2468829/1‑93 (MQ=255)

               aTGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCaaaa                                                                          >  1:2583168/1‑93 (MQ=255)

                tggtggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCaaaaa                                                                         >  2:3931719/1‑93 (MQ=255)

                 ggtggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAg                                                                        <  2:2625759/93‑1 (MQ=255)

                 ggtggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAg                                                                        >  2:348815/1‑93 (MQ=255)

                   tggCGTGTTATTTCTGAAATGATGCTGCATGATTGCTTTTACGGGCACAAGATTGCACGAAGAAAGTACTCGCTGCCACAATGGCAAAAAGaa                                                                      <  2:3813930/93‑1 (MQ=255)

                   tggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGaa                                                                      <  1:1535864/93‑1 (MQ=255)

                    ggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAt                                                                     >  1:4080358/1‑93 (MQ=255)

                    ggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAt                                                                     >  1:99295/1‑93 (MQ=255)

                     gCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATc                                                                    <  2:821240/93‑1 (MQ=255)

                      cGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATca                                                                   >  2:3197877/1‑93 (MQ=255)

                      cGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATca                                                                   <  2:648119/93‑1 (MQ=255)

                      cGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATca                                                                   <  1:2827134/93‑1 (MQ=255)

                       gTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATcac                                                                  >  2:2681232/1‑93 (MQ=255)

                        tGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATcaca                                                                 >  2:3410213/1‑93 (MQ=255)

                         ggTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACaa                                                                >  2:4429102/1‑93 (MQ=255)

                         ggTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACaa                                                                <  2:169708/93‑1 (MQ=255)

                          gTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACaat                                                               >  1:3887136/1‑93 (MQ=255)

                            aaTTCTGAAATGTTGCTGCATGATTGCTTTTACGTACGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAGTCACaataa                                                             <  2:712143/93‑1 (MQ=255)

                                cTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGga                                                         <  1:527146/93‑1 (MQ=255)

                                cTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGga                                                         >  1:3605191/1‑93 (MQ=255)

                                 tGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGgag                                                        >  1:3721688/1‑93 (MQ=255)

                                  gAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAACAAGAATCACAATAATGgaga                                                       >  1:797449/1‑93 (MQ=255)

                                  gAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGgaga                                                       <  2:931552/93‑1 (MQ=255)

                                  gAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGgaga                                                       <  2:4667552/93‑1 (MQ=255)

                                  gAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGgaga                                                       <  2:1839909/93‑1 (MQ=255)

                                    aaTGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATg                                                     >  1:2354420/1‑93 (MQ=255)

                                    aaTGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATg                                                     >  1:4178654/1‑93 (MQ=255)

                                     atgatgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGc                                                    >  1:2855793/1‑93 (MQ=255)

                                      tgatgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCg                                                   >  2:3588766/1‑93 (MQ=255)

                                      tgatgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCg                                                   >  2:3961614/1‑93 (MQ=255)

                                      tgatgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCg                                                   <  2:2809208/93‑1 (MQ=255)

                                      tgatgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCg                                                   <  1:4128427/93‑1 (MQ=255)

                                        atgCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGtt                                                 <  1:4483696/93‑1 (MQ=255)

                                          gctgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTa                                               <  1:304565/93‑1 (MQ=255)

                                           ctgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTAc                                              <  1:4525073/93‑1 (MQ=255)

                                           ctgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTAc                                              <  2:2434986/93‑1 (MQ=255)

                                           ctgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTAc                                              >  2:1906376/1‑93 (MQ=255)

                                            tgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACt                                             <  1:481838/93‑1 (MQ=255)

                                            tgcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACt                                             >  2:2518877/1‑93 (MQ=255)

                                             gcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGCGATGCGTTTACtt                                            >  1:2372676/1‑93 (MQ=255)

                                             gcATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACtt                                            <  2:742821/93‑1 (MQ=255)

                                               aTGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTaa                                          >  1:3996235/1‑93 (MQ=255)

                                               aTGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTaa                                          <  2:1169687/93‑1 (MQ=255)

                                                tGATTGCTTTTCCGGGCGCAAGATTGCACGCAGAAAGCACACGCTGCCACAATGGCAACAAGAATCACAATAATGGAGATGCGTTTACTTAAc                                         >  2:3136531/1‑93 (MQ=255)

                                                  aTTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGa                                       >  1:4590394/1‑93 (MQ=255)

                                                  aTTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGa                                       >  1:1731162/1‑93 (MQ=255)

                                                  aTTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGa                                       >  1:3783146/1‑93 (MQ=255)

                                                  aTTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGa                                       <  1:3367864/93‑1 (MQ=255)

                                                  aTTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATAACAATAATGGAGATGCGTTTACTTAACGa                                       >  2:2893214/1‑93 (MQ=255)

                                                    tGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGaaa                                     <  1:1491184/93‑1 (MQ=255)

                                                       ttttACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAc                                  <  1:1261378/93‑1 (MQ=255)

                                                       ttttACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAc                                  <  2:4115187/93‑1 (MQ=255)

                                                       ttttACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAc                                  <  1:2002915/93‑1 (MQ=255)

                                                        tttACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACt                                 <  1:3000381/93‑1 (MQ=255)

                                                          tACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACttt                               <  2:541685/93‑1 (MQ=255)

                                                          tACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACttt                               <  2:1173260/93‑1 (MQ=255)

                                                           aCGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACtttt                              <  1:3424827/93‑1 (MQ=255)

                                                           aCGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACtttt                              <  2:2177492/93‑1 (MQ=255)

                                                            cGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACttttt                             <  1:1915455/93‑1 (MQ=255)

                                                            cGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACttttt                             <  1:2763560/93‑1 (MQ=255)

                                                            cGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACttttt                             <  1:1468027/93‑1 (MQ=255)

                                                                cgcAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCa                         <  2:4369601/93‑1 (MQ=255)

                                                                    aGATTGCACGAAGAAATTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGt                     <  1:839776/93‑1 (MQ=255)

                                                                      aTTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTaa                   <  1:1725521/93‑1 (MQ=255)

                                                                        tGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATc                 <  1:1212414/93‑1 (MQ=255)

                                                                         gCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATcc                <  2:955337/93‑1 (MQ=255)

                                                                         gCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATcc                <  1:3926747/93‑1 (MQ=255)

                                                                           aCGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCt              <  2:2020884/93‑1 (MQ=255)

                                                                              aagaaAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTa           <  2:3893722/93‑1 (MQ=255)

                                                                               agaaAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTaa          <  2:3663012/93‑1 (MQ=255)

                                                                               agaaAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTaa          <  1:3340002/93‑1 (MQ=255)

                                                                               agaaAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTaa          <  2:3231612/93‑1 (MQ=255)

                                                                                gaaAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAg         <  1:1009190/93‑1 (MQ=255)

                                                                                 aaaGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAgg        <  1:574007/93‑1 (MQ=255)

                                                                                  aaGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGc       <  2:484389/93‑1 (MQ=255)

                                                                                  aaGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGc       <  2:2383086/93‑1 (MQ=255)

                                                                                     tACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  2:434263/93‑1 (MQ=255)

                                                                                     tACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  2:2122306/93‑1 (MQ=255)

                                                                                     tACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  1:688224/93‑1 (MQ=255)

                                                                                     tACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  1:334448/93‑1 (MQ=255)

                                                                                     tACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  1:1756058/93‑1 (MQ=255)

                                                                                     tACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa    <  2:4033885/93‑1 (MQ=255)

                                                                                       cacgcTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATc  <  2:1985673/93‑1 (MQ=255)

                                                                                       cacgcTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATc  <  2:1594251/93‑1 (MQ=255)

ATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATC  >  NC_000913/4561015‑4561194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATA  >  NC_000913/4561012‑4561198

GCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACG                                                                                               >  SRR4302535.767410/1‑94 (MQ=60)

 CAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGC                                                                                              <  SRR4302535.1303779/94‑1 (MQ=60)

 CAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGC                                                                                              >  SRR4302535.4215838/1‑94 (MQ=60)

  AATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCT                                                                                             >  SRR4302535.4352203/1‑94 (MQ=60)

  AATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCT                                                                                             >  SRR4302535.1650552/1‑94 (MQ=60)

  AATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCT                                                                                             <  SRR4302535.3779923/94‑1 (MQ=60)

   ATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTG                                                                                            >  SRR4302535.611278/1‑94 (MQ=60)

   ATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTG                                                                                            >  SRR4302535.4537835/1‑94 (MQ=60)

     TTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCC                                                                                          >  SRR4302535.2219581/1‑94 (MQ=60)

      TCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCA                                                                                         >  SRR4302535.2550725/1‑94 (MQ=60)

      TCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCCGCCA                                                                                         >  SRR4302535.2550724/1‑94 (MQ=60)

       CTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCAC                                                                                        >  SRR4302535.1355559/1‑94 (MQ=60)

         TTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAA                                                                                      >  SRR4302535.4472060/1‑94 (MQ=60)

           GCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATG                                                                                    <  SRR4302535.1203466/94‑1 (MQ=60)

           GCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATG                                                                                    <  SRR4302535.165126/94‑1 (MQ=60)

           GCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATG                                                                                    <  SRR4302535.3753315/94‑1 (MQ=60)

           GCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATG                                                                                    <  SRR4302535.676496/94‑1 (MQ=60)

              GTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCA                                                                                 <  SRR4302535.3680068/94‑1 (MQ=60)

              GTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCA                                                                                 <  SRR4302535.3539384/94‑1 (MQ=60)

              GTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCA                                                                                 <  SRR4302535.209996/94‑1 (MQ=60)

               TCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAA                                                                                <  SRR4302535.3928285/94‑1 (MQ=60)

                 AATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAA                                                                              >  SRR4302535.3526413/1‑94 (MQ=60)

                 AATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAA                                                                              >  SRR4302535.2601728/1‑94 (MQ=60)

                 AATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAA                                                                              >  SRR4302535.2486625/1‑94 (MQ=60)

                   TGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAG                                                                            <  SRR4302535.2868276/94‑1 (MQ=60)

                    GGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGA                                                                           <  SRR4302535.4276989/94‑1 (MQ=60)

                      TGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAT                                                                         >  SRR4302535.4108268/1‑94 (MQ=60)

                      TGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAT                                                                         <  SRR4302535.2718945/94‑1 (MQ=60)

                      TGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAT                                                                         <  SRR4302535.717644/94‑1 (MQ=60)

                      TGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAT                                                                         >  SRR4302535.99994/1‑94 (MQ=60)

                      TGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAAT                                                                         <  SRR4302535.1546930/94‑1 (MQ=60)

                       GGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATC                                                                        <  SRR4302535.2607112/94‑1 (MQ=60)

                       GGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATC                                                                        >  SRR4302535.4334328/1‑94 (MQ=60)

                        GCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCA                                                                       <  SRR4302535.1353599/94‑1 (MQ=60)

                         CGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCAC                                                                      <  SRR4302535.2847199/94‑1 (MQ=60)

                         CGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCAC                                                                      <  SRR4302535.3396504/94‑1 (MQ=60)

                            GGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAAT                                                                   >  SRR4302535.3913797/1‑94 (MQ=60)

                             GTAATTCTGAAATGATGCTGCATGATTGCTTTTACCGGCGCAAGATTGCACGAAGAAAGTAAACGCCGCCACAACGGCAAAAAGAATCACAATA                                                                  >  SRR4302535.1922983/1‑94 (MQ=60)

                               AATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAAT                                                                <  SRR4302535.803164/94‑1 (MQ=60)

                                ATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATG                                                               <  SRR4302535.4462773/94‑1 (MQ=60)

                                  TCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGA                                                             >  SRR4302535.3630200/1‑94 (MQ=60)

                                   CTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGACAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAG                                                            <  SRR4302535.2528354/94‑1 (MQ=60)

                                   CTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAG                                                            >  SRR4302535.3747472/1‑94 (MQ=60)

                                   CTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAG                                                            >  SRR4302535.4597794/1‑94 (MQ=60)

                                   CTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAG                                                            <  SRR4302535.530829/94‑1 (MQ=60)

                                    TGAAATGATGCTGCATGATTGCTTTTACGGGGGAAAGATTGCCGGAAGAAAGTACACGCTGCCACAAT‑GCAAAAAGAATCACAATAATGGAGAT                                                          <  SRR4302535.3166185/94‑1 (MQ=60)

                                    TGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAACAAGAATCACAATAATGGAGA                                                           >  SRR4302535.803048/1‑94 (MQ=60)

                                    TGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGA                                                           >  SRR4302535.2018142/1‑94 (MQ=60)

                                    TGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGA                                                           >  SRR4302535.4117837/1‑94 (MQ=60)

                                      AAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATG                                                         >  SRR4302535.4207256/1‑94 (MQ=60)

                                      AAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATG                                                         >  SRR4302535.2371299/1‑94 (MQ=60)

                                       AATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGC                                                        >  SRR4302535.2876029/1‑94 (MQ=60)

                                        ATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCG                                                       <  SRR4302535.66628/94‑1 (MQ=60)

                                        ATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCG                                                       <  SRR4302535.1666414/94‑1 (MQ=60)

                                         TGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGT                                                      <  SRR4302535.4156678/94‑1 (MQ=60)

                                          GATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTT                                                     <  SRR4302535.3917802/94‑1 (MQ=60)

                                           ATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTT                                                    <  SRR4302535.4514471/94‑1 (MQ=60)

                                           ATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTT                                                    <  SRR4302535.2506292/94‑1 (MQ=60)

                                             GCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTAC                                                  <  SRR4302535.306678/94‑1 (MQ=60)

                                             GCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTAC                                                  <  SRR4302535.1605904/94‑1 (MQ=60)

                                              CTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACT                                                 >  SRR4302535.47777/1‑94 (MQ=60)

                                              CTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACT                                                 <  SRR4302535.228589/94‑1 (MQ=60)

                                              CTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACT                                                 <  SRR4302535.4556072/94‑1 (MQ=60)

                                              CTGCACGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACT                                                 >  SRR4302535.698644/1‑94 (MQ=60)

                                               TGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGCGATGCGTTTACTT                                                >  SRR4302535.2389718/1‑94 (MQ=60)

                                               TGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTT                                                >  SRR4302535.2365601/1‑94 (MQ=60)

                                               TGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTT                                                <  SRR4302535.2377012/94‑1 (MQ=60)

                                               TGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTT                                                <  SRR4302535.485220/94‑1 (MQ=60)

                                                GCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTA                                               <  SRR4302535.1752732/94‑1 (MQ=60)

                                                GCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTA                                               <  SRR4302535.3415936/94‑1 (MQ=60)

                                                 CATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAA                                              <  SRR4302535.3969245/94‑1 (MQ=60)

                                                 CATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAA                                              >  SRR4302535.4023578/1‑94 (MQ=60)

                                                  ATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAAC                                             <  SRR4302535.531505/94‑1 (MQ=60)

                                                   TGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACG                                            <  SRR4302535.1752839/94‑1 (MQ=60)

                                                    GATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGA                                           >  SRR4302535.1743677/1‑94 (MQ=60)

                                                    GATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGA                                           >  SRR4302535.4621640/1‑94 (MQ=60)

                                                    GATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGA                                           >  SRR4302535.3809248/1‑94 (MQ=60)

                                                     ATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAA                                          <  SRR4302535.3391298/94‑1 (MQ=60)

                                                     ATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAA                                          >  SRR4302535.4623521/1‑94 (MQ=60)

                                                     ATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAA                                          <  SRR4302535.149020/94‑1 (MQ=60)

                                                       TGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATTACAATAATGGAGATGCGTTTACTTAACGAAAT                                        <  SRR4302535.3853668/94‑1 (MQ=60)

                                                       TGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAAT                                        <  SRR4302535.1501891/94‑1 (MQ=60)

                                                       TGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAAT                                        <  SRR4302535.148537/94‑1 (MQ=60)

                                                       TGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAAT                                        <  SRR4302535.2162240/94‑1 (MQ=60)

                                                        GCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATA                                       <  SRR4302535.1520229/94‑1 (MQ=60)

                                                         CTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAT                                      >  SRR4302535.3805080/1‑94 (MQ=60)

                                                         CTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAC                                      <  SRR4302535.2482980/94‑1 (MQ=60)

                                                         CTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATAC                                      >  SRR4302535.3065953/1‑94 (MQ=60)

                                                          TTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACT                                     <  SRR4302535.1270403/94‑1 (MQ=60)

                                                          TTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACT                                     <  SRR4302535.3230974/94‑1 (MQ=60)

                                                          TTTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACT                                     <  SRR4302535.2017348/94‑1 (MQ=60)

                                                           TTTACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTT                                    <  SRR4302535.3021415/94‑1 (MQ=60)

                                                             TACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTT                                  <  SRR4302535.1457063/94‑1 (MQ=60)

                                                              ACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTT                                 <  SRR4302535.3448643/94‑1 (MQ=60)

                                                              ACGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTT                                 <  SRR4302535.4179216/94‑1 (MQ=60)

                                                               CGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTG                                >  SRR4302535.679804/1‑94 (MQ=60)

                                                               CGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTG                                <  SRR4302535.2783249/94‑1 (MQ=60)

                                                               CGGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTG                                <  SRR4302535.1929258/94‑1 (MQ=60)

                                                               CGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTG                                <  SRR4302535.1478543/94‑1 (MQ=60)

                                                                GGGCGCAAGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGT                               >  SRR4302535.2934506/1‑94 (MQ=60)

                                                                       AGATTGCACGAAGAAATTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTA                        <  SRR4302535.845674/94‑1 (MQ=60)

                                                                       AGATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTA                        <  SRR4302535.1938887/94‑1 (MQ=60)

                                                                        GATTGCACGAAGAAAGTACGCGCTGACACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAA                       <  SRR4302535.4128708/94‑1 (MQ=60)

                                                                         ATTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAAT                      <  SRR4302535.1737999/94‑1 (MQ=60)

                                                                         ATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAAT                      >  SRR4302535.3630117/1‑94 (MQ=60)

                                                                          TTGCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATC                     >  SRR4302535.363583/1‑94 (MQ=60)

                                                                           TGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCC                    <  SRR4302535.1221112/94‑1 (MQ=60)

                                                                            GCACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCC                   <  SRR4302535.3953572/94‑1 (MQ=60)

                                                                             CACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCT                  <  SRR4302535.1281137/94‑1 (MQ=60)

                                                                             CACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCT                  <  SRR4302535.3364911/94‑1 (MQ=60)

                                                                              ACGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTT                 <  SRR4302535.4043234/94‑1 (MQ=60)

                                                                               CGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTT                <  SRR4302535.3385637/94‑1 (MQ=60)

                                                                               CGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTT                <  SRR4302535.2966684/94‑1 (MQ=60)

                                                                               CGAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTT                <  SRR4302535.449004/94‑1 (MQ=60)

                                                                                GAAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTA               >  SRR4302535.1243032/1‑94 (MQ=60)

                                                                                 AAGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAA              >  SRR4302535.491918/1‑94 (MQ=60)

                                                                                  AGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAG             <  SRR4302535.3363277/94‑1 (MQ=60)

                                                                                  AGAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAG             <  SRR4302535.2844116/94‑1 (MQ=60)

                                                                                   GAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGG            <  SRR4302535.1016360/94‑1 (MQ=60)

                                                                                   GAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGG            <  SRR4302535.3697871/94‑1 (MQ=60)

                                                                                   GAAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGG            <  SRR4302535.2031073/94‑1 (MQ=60)

                                                                                    AAAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGC           <  SRR4302535.578035/94‑1 (MQ=60)

                                                                                     AAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCA          >  SRR4302535.1742587/1‑94 (MQ=60)

                                                                                     AAGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCA          <  SRR4302535.893776/94‑1 (MQ=60)

                                                                                      AGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAA         >  SRR4302535.455096/1‑94 (MQ=60)

                                                                                      AGTACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAA         >  SRR4302535.3457650/1‑94 (MQ=60)

                                                                                        TACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAAT       <  SRR4302535.693044/94‑1 (MQ=60)

                                                                                        TACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAAT       <  SRR4302535.336761/94‑1 (MQ=60)

                                                                                        TACACGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAAT       <  SRR4302535.1768751/94‑1 (MQ=60)

                                                                                            CGCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAAT   <  SRR4302535.458388/94‑1 (MQ=60)

                                                                                             GCTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATA  <  SRR4302535.2206021/94‑1 (MQ=60)

GCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATA  >  NC_000913/4561012‑4561198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`