Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,593,336	+G	intergenic (‑23/‑208)	yjjM ← / → yjjN	hypothetical protein/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,593,335	1	.	G	100.0%	614.3 / NA	192	intergenic (‑22/‑209)	yjjM/yjjN	hypothetical protein/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (82/110); total (82/110)

Only 100 of 213 total aligned reads displayed.

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422

 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑g                                                                                        <  1:981137/93‑1 (MQ=255)

 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑g                                                                                        >  1:354191/1‑93 (MQ=255)

    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     <  1:1089166/93‑4 (MQ=255)

    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     >  1:1286846/1‑90 (MQ=255)

    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaa                                                                                     <  2:1004864/93‑4 (MQ=255)

     cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑ggaag                                                                                    <  2:2354036/93‑5 (MQ=255)

         acgccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTggg                                                                                 <  2:3239439/89‑1 (MQ=255)

          cgccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTgggg                                                                                <  2:3585975/90‑1 (MQ=255)

           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGa                                                                               <  1:919199/91‑1 (MQ=255)

           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGa                                                                               >  2:2447445/3‑93 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGaa                                                                              <  2:3659613/92‑1 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGaa                                                                              >  1:1100526/2‑93 (MQ=255)

             cgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAg                                                                             <  1:841368/93‑1 (MQ=255)

              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAgg                                                                            <  2:1355580/93‑1 (MQ=255)

               cATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAggg                                                                           >  1:1197660/1‑93 (MQ=255)

               cATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAggg                                                                           >  2:679680/1‑93 (MQ=255)

                aTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGa                                                                          >  1:1551278/1‑93 (MQ=255)

                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGat                                                                         >  2:617863/1‑93 (MQ=255)

                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGat                                                                         <  1:4376802/93‑1 (MQ=255)

                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGat                                                                         >  1:2029905/1‑93 (MQ=255)

                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGat                                                                         <  1:2806429/93‑1 (MQ=255)

                   cgcgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGatat                                                                       >  1:3468621/1‑93 (MQ=255)

                    gcgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATg                                                                      >  2:402517/1‑93 (MQ=255)

                    gcgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATg                                                                      >  2:2795674/1‑93 (MQ=255)

                     cgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGa                                                                     >  1:1271417/1‑93 (MQ=255)

                      gTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAc                                                                    <  2:3088549/93‑1 (MQ=255)

                      gTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAc                                                                    >  2:3683612/1‑93 (MQ=255)

                      gTAAGGGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAc                                                                    >  2:3908053/1‑93 (MQ=255)

                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAcc                                                                   <  2:4160479/93‑1 (MQ=255)

                         aGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGAcccc                                                                 <  1:2918462/93‑1 (MQ=255)

                          gTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCcg                                                                >  2:907308/1‑93 (MQ=255)

                          gTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGGGGGGAAGGGATATGACCCcg                                                                >  2:1467643/1‑93 (MQ=255)

                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCa                                                           >  2:57333/1‑93 (MQ=255)

                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCa                                                           >  1:3775476/1‑93 (MQ=255)

                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATACGACCCCGCGCCa                                                           >  2:2835338/1‑93 (MQ=255)

                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                          <  1:3928250/93‑1 (MQ=255)

                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                         <  2:3745875/93‑1 (MQ=255)

                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                         <  1:1783131/93‑1 (MQ=255)

                                  gaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                        <  1:194090/93‑1 (MQ=255)

                                  gaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                        <  2:3007764/93‑1 (MQ=255)

                                   aaagtGACGCACGGTGAGGCTGCTGAGGTTGTACATTTCCGCCAGCGAACATTGCGAGG‑GAAGTGGGGAATGGATATGGCCCCGCGCCATatc                                                       <  2:750427/89‑1 (MQ=255)

                                   aaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                       <  1:4179713/93‑1 (MQ=255)

                                   aaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                       <  2:2757709/93‑1 (MQ=255)

                                   aaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                       <  1:1414482/93‑1 (MQ=255)

                                   aaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                       <  1:1456088/93‑1 (MQ=255)

                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      >  1:4000941/1‑93 (MQ=255)

                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                      >  2:1754542/1‑93 (MQ=255)

                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     >  2:2181738/1‑93 (MQ=255)

                                      atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcatc                                                    <  2:4247777/93‑1 (MQ=255)

                                      atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcatc                                                    <  1:58351/93‑1 (MQ=255)

                                       tGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATAtcatca                                                   >  2:3057357/1‑93 (MQ=255)

                                        gACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCaa                                                  <  1:717765/93‑1 (MQ=255)

                                        gACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCaa                                                  >  2:3804413/1‑93 (MQ=255)

                                        gACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCaa                                                  <  1:381184/93‑1 (MQ=255)

                                         aCGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                 <  2:2656575/93‑1 (MQ=255)

                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                               <  1:2327565/93‑1 (MQ=255)

                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                               <  2:3473091/93‑1 (MQ=255)

                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                               >  1:3144807/1‑93 (MQ=255)

                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                              >  1:669707/1‑93 (MQ=255)

                                               tgtgGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTg                                           >  2:4520643/1‑93 (MQ=255)

                                               tgtgGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTg                                           <  1:3281942/93‑1 (MQ=255)

                                                gtggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTgg                                          <  1:4226619/93‑1 (MQ=255)

                                                  ggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGtt                                        >  2:2599182/1‑93 (MQ=255)

                                                   gtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTa                                       >  2:1252282/1‑93 (MQ=255)

                                                   gtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTa                                       <  1:3085283/93‑1 (MQ=255)

                                                    tgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTaa                                      <  1:1861600/93‑1 (MQ=255)

                                                     gCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAt                                     <  1:1486759/93‑1 (MQ=255)

                                                      gggCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATc                                    <  1:1006878/92‑1 (MQ=255)

                                                      cGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATc                                    >  1:1632556/1‑93 (MQ=255)

                                                      cGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATc                                    <  1:2870839/93‑1 (MQ=255)

                                                        gCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcac                                  >  2:1971877/1‑93 (MQ=255)

                                                         cTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcaca                                 <  2:1073339/93‑1 (MQ=255)

                                                         cTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTAGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcaca                                 >  1:3298489/1‑93 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGTAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                >  1:2611914/1‑93 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  1:2179354/93‑1 (MQ=255)

                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                               >  1:2387307/1‑93 (MQ=255)

                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                               >  2:1510134/1‑93 (MQ=255)

                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgt                             <  1:2130268/93‑1 (MQ=255)

                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgt                             <  2:4300983/93‑1 (MQ=255)

                                                              gttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgtg                            <  2:3770714/93‑1 (MQ=255)

                                                              gttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGCGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgtg                            <  1:791578/93‑1 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  1:2681113/93‑1 (MQ=255)

                                                                  tACATTTCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGt                        <  2:4170141/93‑1 (MQ=255)

                                                                        tCCGCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAttt                  <  1:2230629/93‑1 (MQ=255)

                                                                           gCCAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgt               >  1:2744259/1‑93 (MQ=255)

                                                                            ccAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgtg              <  2:1270399/93‑1 (MQ=255)

                                                                             cAGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGa             >  1:863246/1‑93 (MQ=255)

                                                                              aGCGCACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGaa            <  2:408117/93‑1 (MQ=255)

                                                                               gcgcACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAc           >  2:1695261/1‑93 (MQ=255)

                                                                                  cACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAc        <  2:2651331/93‑1 (MQ=255)

                                                                                  cACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAc        >  1:4585408/1‑93 (MQ=255)

                                                                                  cACTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAc        >  2:273338/1‑93 (MQ=255)

                                                                                   aCTTTGCGA‑TGGAAGTGGGGAAGGGATATTACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt       <  2:3349132/93‑1 (MQ=255)

                                                                                   aCTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt       >  2:3322587/1‑93 (MQ=255)

                                                                                   aCTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt       <  1:2156219/93‑1 (MQ=255)

                                                                                    cTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc      >  2:4606604/1‑93 (MQ=255)

                                                                                    cTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc      <  2:2340395/93‑1 (MQ=255)

                                                                                    cTTTGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc      <  1:1334399/93‑1 (MQ=255)

                                                                                     tttGCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCa     >  1:2590306/1‑93 (MQ=255)

                                                                                        gCGA‑TGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCatat  <  1:4112925/93‑1 (MQ=255)

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA‑T‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429

TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G                                                                                               >  SRR4302535.356635/1‑94 (MQ=60)

CGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G                                                                                               <  SRR4302535.1073730/94‑1 (MQ=60)

 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG                                                                                              <  SRR4302535.988109/94‑1 (MQ=60)

 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG                                                                                              <  SRR4302535.2259420/94‑1 (MQ=60)

 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG                                                                                              >  SRR4302535.2901117/1‑94 (MQ=60)

   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                            >  SRR4302535.1296015/1‑91 (MQ=60)

    TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAG                                                                                            <  SRR4302535.1096918/94‑1 (MQ=60)

     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGT                                                                                           >  SRR4302535.3071418/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                          >  SRR4302535.4219847/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                          >  SRR4302535.4040147/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                          >  SRR4302535.3130039/1‑94 (MQ=60)

       AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG                                                                                         <  SRR4302535.1044758/94‑1 (MQ=60)

       AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG                                                                                         >  SRR4302535.3287622/1‑94 (MQ=60)

         acgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGG                                                                                       <  SRR4302535.3033793/90‑1 (MQ=60)

           gcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAA                                                                                     <  SRR4302535.925719/92‑1 (MQ=60)

           gcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAA                                                                                     >  SRR4302535.1108386/3‑94 (MQ=60)

             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGG                                                                                   <  SRR4302535.847272/94‑1 (MQ=60)

              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGG                                                                                  >  SRR4302535.1206236/1‑94 (MQ=60)

               CATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGA                                                                                 >  SRR4302535.1562448/1‑94 (MQ=60)

               CATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGA                                                                                 <  SRR4302535.3142313/94‑1 (MQ=60)

                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGAT                                                                                >  SRR4302535.2044536/1‑94 (MQ=60)

                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGAT                                                                                <  SRR4302535.480987/94‑1 (MQ=60)

                 TTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATA                                                                               <  SRR4302535.2826380/94‑1 (MQ=60)

                 TTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATA                                                                               <  SRR4302535.4406748/94‑1 (MQ=60)

                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAT                                                                              >  SRR4302535.3492724/1‑94 (MQ=60)

                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAT                                                                              <  SRR4302535.245040/94‑1 (MQ=60)

                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAT                                                                              <  SRR4302535.1844909/94‑1 (MQ=60)

                   CGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATG                                                                             <  SRR4302535.740868/94‑1 (MQ=60)

                    GCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGA                                                                            >  SRR4302535.1280500/1‑94 (MQ=60)

                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACC                                                                          >  SRR4302535.1759444/1‑94 (MQ=60)

                        AAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCC                                                                        >  SRR4302535.3265629/1‑94 (MQ=60)

                         AGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCG                                                                       <  SRR4302535.2939058/94‑1 (MQ=60)

                          GTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGC                                                                      >  SRR4302535.3397429/1‑94 (MQ=60)

                            GGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGC                                                                    >  SRR4302535.3661512/1‑94 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                  >  SRR4302535.261301/1‑94 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                  >  SRR4302535.3801507/1‑94 (MQ=60)

                                GAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATA                                                                <  SRR4302535.127455/94‑1 (MQ=60)

                                GAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATA                                                                <  SRR4302535.3955081/94‑1 (MQ=60)

                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302535.4376799/94‑1 (MQ=60)

                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302535.1796038/94‑1 (MQ=60)

                                  GAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC                                                              >  SRR4302535.2785094/1‑94 (MQ=60)

                                  GAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC                                                              <  SRR4302535.195427/94‑1 (MQ=60)

                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                             <  SRR4302535.4208320/94‑1 (MQ=60)

                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                             <  SRR4302535.1466510/94‑1 (MQ=60)

                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                             <  SRR4302535.1424579/94‑1 (MQ=60)

                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                             >  SRR4302535.4028311/1‑94 (MQ=60)

                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            <  SRR4302535.1175190/94‑1 (MQ=60)

                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                           >  SRR4302535.1518560/1‑94 (MQ=60)

                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                           <  SRR4302535.269731/94‑1 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302535.898950/94‑1 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          >  SRR4302535.3947772/1‑94 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302535.4119415/94‑1 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302535.58780/94‑1 (MQ=60)

                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        <  SRR4302535.722807/94‑1 (MQ=60)

                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        <  SRR4302535.383820/94‑1 (MQ=60)

                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGA                                                      >  SRR4302535.3166678/1‑94 (MQ=60)

                                           GCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAC                                                     >  SRR4302535.674381/1‑94 (MQ=60)

                                           GCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAC                                                     <  SRR4302535.2344218/94‑1 (MQ=60)

                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    >  SRR4302535.1314108/1‑94 (MQ=60)

                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   >  SRR4302535.2382621/1‑94 (MQ=60)

                                             ACTGTGGTGCGGCTGATGTTGCACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302535.4424980/94‑1 (MQ=60)

                                              CTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTG                                                  <  SRR4302535.1289041/94‑1 (MQ=60)

                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 <  SRR4302535.3317039/94‑1 (MQ=60)

                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 <  SRR4302535.3304735/94‑1 (MQ=60)

                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                <  SRR4302535.4255542/94‑1 (MQ=60)

                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                <  SRR4302535.4193901/94‑1 (MQ=60)

                                                   GTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAA                                             <  SRR4302535.3106776/94‑1 (MQ=60)

                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                            <  SRR4302535.1875025/94‑1 (MQ=60)

                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           >  SRR4302535.1644352/1‑94 (MQ=60)

                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302535.1497406/94‑1 (MQ=60)

                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                           <  SRR4302535.1104998/94‑1 (MQ=60)

                                                      GGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          <  SRR4302535.1014035/94‑1 (MQ=60)

                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGTGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          >  SRR4302535.2700591/1‑94 (MQ=60)

                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          <  SRR4302535.2891179/94‑1 (MQ=60)

                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          <  SRR4302535.716115/94‑1 (MQ=60)

                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                        <  SRR4302535.2065699/94‑1 (MQ=60)

                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTAGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                        >  SRR4302535.3321425/1‑94 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGTAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302535.2630650/1‑94 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       <  SRR4302535.4217588/94‑1 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       <  SRR4302535.857728/94‑1 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGGGGGGAAGGGATATGACCCCCGGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302535.1004031/1‑94 (MQ=60)

                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      >  SRR4302535.2404470/1‑94 (MQ=60)

                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      <  SRR4302535.2194912/94‑1 (MQ=60)

                                                           GATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTG                                     <  SRR4302535.3836786/94‑1 (MQ=60)

                                                           GATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTG                                     >  SRR4302535.2884167/1‑94 (MQ=60)

                                                            ATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGT                                    >  SRR4302535.2067001/1‑94 (MQ=60)

                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                   <  SRR4302535.2145624/94‑1 (MQ=60)

                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  >  SRR4302535.2637245/1‑94 (MQ=60)

                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  >  SRR4302535.2014315/1‑94 (MQ=60)

                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATAACCTGGTTAATCACATTGTGG                                  <  SRR4302535.2158512/94‑1 (MQ=60)

                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGCGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  <  SRR4302535.797132/94‑1 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302535.1375195/94‑1 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302535.1463306/94‑1 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 <  SRR4302535.2700294/94‑1 (MQ=60)

                                                                   ACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAA                             >  SRR4302535.650695/1‑94 (MQ=60)

                                                                     ATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCAGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAT                           <  SRR4302535.2237387/94‑1 (MQ=60)

                                                                       TTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                         <  SRR4302535.1461134/94‑1 (MQ=60)

                                                                        TCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        <  SRR4302535.2246490/94‑1 (MQ=60)

                                                                          CGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGT                      >  SRR4302535.2763821/1‑94 (MQ=60)

                                                                            CCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGA                    >  SRR4302535.869347/1‑94 (MQ=60)

                                                                             CAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAA                   >  SRR4302535.3286438/1‑94 (MQ=60)

                                                                             CAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAA                   <  SRR4302535.163457/94‑1 (MQ=60)

                                                                              AGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                  <  SRR4302535.741255/94‑1 (MQ=60)

                                                                               GCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACG                 <  SRR4302535.948357/94‑1 (MQ=60)

                                                                                CGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA                <  SRR4302535.2185035/94‑1 (MQ=60)

                                                                                CGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA                >  SRR4302535.826539/1‑94 (MQ=60)

                                                                                 GCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAC               <  SRR4302535.4098906/94‑1 (MQ=60)

                                                                                 GCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAC               >  SRR4302535.4616633/1‑94 (MQ=60)

                                                                                  CACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAGGACT              >  SRR4302535.1484413/1‑94 (MQ=60)

                                                                                  CACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT              >  SRR4302535.3545553/1‑94 (MQ=60)

                                                                                  CACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT              >  SRR4302535.188070/1‑94 (MQ=60)

                                                                                   ACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC             <  SRR4302535.2171711/94‑1 (MQ=60)

                                                                                    CTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302535.3984082/1‑94 (MQ=60)

                                                                                    CTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302535.2608906/1‑94 (MQ=60)

                                                                                    CTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            <  SRR4302535.1343917/94‑1 (MQ=60)

                                                                                        GCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATT        <  SRR4302535.4141079/94‑1 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT       <  SRR4302535.1712935/94‑1 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT       >  SRR4302535.3921449/1‑94 (MQ=60)

                                                                                          GATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTT      >  SRR4302535.3222713/1‑94 (MQ=60)

                                                                                           atgGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTC     >  SRR4302535.4527830/4‑94 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   <  SRR4302535.638934/94‑1 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   <  SRR4302535.1815084/94‑1 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   <  SRR4302535.2535762/94‑1 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   >  SRR4302535.3811532/1‑94 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   >  SRR4302535.2717929/1‑94 (MQ=60)

                                                                                              GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  <  SRR4302535.4240319/94‑1 (MQ=60)

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`