Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 652,748 A→G T431A (ACC→GCC)  citA → putative sensor‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913652,7480AG99.6% 856.8 / NA 273T431A (ACC→GCC) citAputative sensor‑type protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (138/134);  minor base T (0/1);  total (138/135)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCACCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTA  >  NC_000913/652658‑652837
                                                                                          |                                                                                         
ggTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGcc                                                                                         <  1:9500515/93‑1 (MQ=255)
  tAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGa                                                                                       <  2:6416108/93‑1 (MQ=255)
   aaaGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAg                                                                                      >  1:4264982/1‑93 (MQ=255)
    aaGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGt                                                                                     >  1:9235938/1‑93 (MQ=255)
      gTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGttt                                                                                   <  1:8852914/93‑1 (MQ=255)
         cAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCa                                                                                <  2:4147301/93‑1 (MQ=255)
           gcgcgcCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGc                                                                              >  1:4006000/1‑93 (MQ=255)
             gcgcCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCa                                                                            >  1:8926118/1‑93 (MQ=255)
              cgcCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCAt                                                                           <  2:3777778/93‑1 (MQ=255)
              cgcCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCAt                                                                           <  2:8218841/93‑1 (MQ=255)
              cgcCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCAt                                                                           >  1:9187084/1‑93 (MQ=255)
                cccGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATtg                                                                         <  2:3229092/93‑1 (MQ=255)
                  cGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCCGCCATtgtg                                                                       >  1:5805819/1‑93 (MQ=255)
                  cGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATtgtg                                                                       >  1:2017152/1‑93 (MQ=255)
                  cGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATtgtg                                                                       <  1:6555748/93‑1 (MQ=255)
                  cGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATtgtg                                                                       >  1:4654440/1‑93 (MQ=255)
                  cGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATtgtg                                                                       <  2:9147874/93‑1 (MQ=255)
                   gggAACTGAGTCTAAAAATGATCATTGTACCCGGTAGCCAGCTTACGCAACTGCCTCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTgg                                                                      <  1:7016057/93‑1 (MQ=255)
                    ggAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTggg                                                                     >  2:2525508/1‑93 (MQ=255)
                    ggAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTggg                                                                     >  1:1138576/1‑93 (MQ=255)
                     gAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGc                                                                    >  2:6205651/1‑93 (MQ=255)
                     gAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGc                                                                    >  2:8891820/1‑93 (MQ=255)
                      aaCTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCa                                                                   >  2:9050208/1‑93 (MQ=255)
                       aCTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCaa                                                                  <  1:2533790/93‑1 (MQ=255)
                       aCTGGGGCTAAAAATGATCATCGTCCCCGGTAGCCAGCTTTCGCAACTTCCGCCAGGACTGGATAGCGCCGAGGTTGCAGCCACTGTGTGCaa                                                                  >  2:4838119/1‑93 (MQ=255)
                        cTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCATCCATTGTGGGCAAt                                                                 >  2:8467239/1‑93 (MQ=255)
                        cTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAt                                                                 <  1:6064985/93‑1 (MQ=255)
                         tGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAtt                                                                <  1:4372379/93‑1 (MQ=255)
                          ggggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAttt                                                               >  1:2794985/1‑93 (MQ=255)
                          ggggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAttt                                                               >  1:3518308/1‑93 (MQ=255)
                           gggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTa                                                              >  1:5572172/1‑93 (MQ=255)
                           gggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTa                                                              >  2:9919429/1‑93 (MQ=255)
                           gggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTa                                                              >  1:6378/1‑93 (MQ=255)
                            ggCTAAAAATTATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTAc                                                             <  2:3607240/93‑1 (MQ=255)
                            ggCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTAc                                                             <  1:4868094/93‑1 (MQ=255)
                             gCTAAAAATGATCATTTTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACt                                                            <  2:2753336/93‑1 (MQ=255)
                             gCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACt                                                            >  2:6445816/1‑93 (MQ=255)
                               tAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTg                                                          >  2:9501445/1‑93 (MQ=255)
                               tAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTg                                                          >  2:4463938/1‑93 (MQ=255)
                               tAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTg                                                          >  1:242499/1‑93 (MQ=255)
                                aagaaTGATCATTGTCCCCGGTAGACCGCTTTTGGAACTGCCGCCAAGACTGGATAGCGCCAAGTTTGGGGTCGTTGTGGGGAAAATCCTTGa                                                         >  2:4474459/4‑93 (MQ=255)
                                aaaaaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGa                                                         <  2:1874251/93‑1 (MQ=255)
                                 aaaaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGAt                                                        >  2:2311435/1‑93 (MQ=255)
                                  aaaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATa                                                       <  1:2387753/93‑1 (MQ=255)
                                  aaaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATa                                                       <  2:435911/93‑1 (MQ=255)
                                   aaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATaa                                                      <  2:7784220/93‑1 (MQ=255)
                                   aaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATaa                                                      >  1:7150889/1‑93 (MQ=255)
                                   aaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATaa                                                      <  2:5845345/93‑1 (MQ=255)
                                   aaTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATaa                                                      <  2:2209110/93‑1 (MQ=255)
                                    aTGATCATTGGCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAAc                                                     <  1:1407140/93‑1 (MQ=255)
                                      gATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGc                                                   >  1:3835426/1‑93 (MQ=255)
                                      gATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGc                                                   <  2:8205649/93‑1 (MQ=255)
                                       aTCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGcc                                                  >  1:815784/1‑93 (MQ=255)
                                       aTCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGcc                                                  <  1:5947122/93‑1 (MQ=255)
                                        tCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCt                                                 <  1:8657977/93‑1 (MQ=255)
                                         cATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCtt                                                <  1:6447918/93‑1 (MQ=255)
                                         cATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCtt                                                <  1:5009620/93‑1 (MQ=255)
                                              tCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAag                                           <  1:1693/93‑1 (MQ=255)
                                                 ccGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagcca                                        >  1:2667523/1‑93 (MQ=255)
                                                 ccGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagcca                                        >  2:7308826/1‑93 (MQ=255)
                                                    gTAGCCGGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagccagcc                                     <  1:9972633/93‑1 (MQ=255)
                                                    gTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagccagcc                                     >  1:4521315/1‑93 (MQ=255)
                                                    gTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagccagcc                                     <  1:7499840/93‑1 (MQ=255)
                                                    gTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAagccagcc                                     >  1:896936/1‑93 (MQ=255)
                                                     tAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCt                                    >  1:5726938/1‑93 (MQ=255)
                                                     tAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCt                                    >  1:7574514/1‑93 (MQ=255)
                                                     tAGCCAGCTTTCGCAACTGCCGCAAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCt                                    <  2:5608385/93‑1 (MQ=255)
                                                       gCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCG‑GGTTGCAGGCCTTTGGGGCAAATTAATTTATAACGCCCTTCAAGCCCGCCcgcg                                 >  1:1387423/1‑93 (MQ=255)
                                                        ccAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCg                                 >  1:5193283/1‑93 (MQ=255)
                                                         cAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGt                                <  2:525243/93‑1 (MQ=255)
                                                          aGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGACGCCAGCCTGCGTa                               >  2:8330363/1‑93 (MQ=255)
                                                           tcTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAg                              <  1:7763471/92‑1 (MQ=255)
                                                           gCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAg                              >  1:1033934/1‑93 (MQ=255)
                                                            cTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGc                             <  2:1775261/93‑1 (MQ=255)
                                                              ttCGCAACTGCCGCCAGGACTGGATAGCTCCGAGTTTGCAGCCATTGTGGGCAATTTCCTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGa                           <  1:5299191/93‑1 (MQ=255)
                                                              ttCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGa                           <  2:3273188/93‑1 (MQ=255)
                                                                    aCTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAgg                     >  2:9208980/1‑93 (MQ=255)
                                                                    aCTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAgg                     >  1:1587892/1‑93 (MQ=255)
                                                                    aCTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAgg                     <  1:8491970/93‑1 (MQ=255)
                                                                     cTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGa                    >  1:3652348/1‑93 (MQ=255)
                                                                      tGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGaa                   >  2:2873814/1‑93 (MQ=255)
                                                                       gccgccAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGaaa                  <  2:2036201/93‑1 (MQ=255)
                                                                         cgccAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACa                <  2:706518/93‑1 (MQ=255)
                                                                         cgccAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACa                <  2:1024561/93‑1 (MQ=255)
                                                                           ccAGGACTGTATAGCGCGGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAg              <  2:9704583/93‑1 (MQ=255)
                                                                           ccAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAg              <  2:654781/93‑1 (MQ=255)
                                                                             agaaCTGGATAGCGCCGAGTTTGCAGCGATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGAt            <  2:1255018/90‑1 (MQ=255)
                                                                             aGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGGAGGAAACAAGAt            >  2:1125122/1‑93 (MQ=255)
                                                                             aGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGAt            <  1:6160674/93‑1 (MQ=255)
                                                                             aGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGAt            <  2:4020336/93‑1 (MQ=255)
                                                                              ggACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATc           >  1:3035784/1‑93 (MQ=255)
                                                                                 cTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGtt        <  1:7343105/93‑1 (MQ=255)
                                                                                  tGGATAGCGCCGAGTTTGCAGCCATTGTGTGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTg       <  1:4121169/93‑1 (MQ=255)
                                                                                   ggATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGa      <  1:9593880/93‑1 (MQ=255)
                                                                                    gATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGaa     >  1:8673305/1‑93 (MQ=255)
                                                                                     aTAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAat    <  2:853915/93‑1 (MQ=255)
                                                                                     aTAGCGCCGAGTTTGCAGCCATTCTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAat    <  2:5510878/93‑1 (MQ=255)
                                                                                      tAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAatt   <  1:1222939/93‑1 (MQ=255)
                                                                                      tAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAatt   >  2:6393176/1‑93 (MQ=255)
                                                                                       aGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAattt  >  1:6002446/1‑92 (MQ=255)
                                                                                          |                                                                                         
GGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCACCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTA  >  NC_000913/652658‑652837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCACCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCC  >  NC_000913/652656‑652841
                                                                                            |                                                                                             
TTGGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGC                                                                                              >  SRR4302540.3125674/1‑94 (MQ=60)
 TGGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCC                                                                                             >  SRR4302540.9346809/1‑94 (MQ=60)
 TGGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCC                                                                                             >  SRR4302540.4692200/1‑94 (MQ=60)
  GGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCG                                                                                            <  SRR4302540.3052226/94‑1 (MQ=60)
  GGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCG                                                                                            <  SRR4302540.9613092/94‑1 (MQ=60)
   GTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGA                                                                                           >  SRR4302540.6246359/1‑94 (MQ=60)
   GTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGA                                                                                           <  SRR4302540.7736120/94‑1 (MQ=60)
   GTAAAGTGCAGCGCGCCCGGGAACTGGGGATAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCATCTGCCGCCAGGACTGGATAGCGCCGA                                                                                           <  SRR4302540.3455024/94‑1 (MQ=60)
    TAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAG                                                                                          >  SRR4302540.4311367/1‑94 (MQ=60)
     AAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGT                                                                                         >  SRR4302540.9346601/1‑94 (MQ=60)
      AAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTT                                                                                        <  SRR4302540.5331146/94‑1 (MQ=60)
       AGTGTAGCGCGCCAGGGAACTGGGGATAAAAATGATCAATGACCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTT                                                                                       <  SRR4302540.2193677/94‑1 (MQ=60)
        GTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTG                                                                                      <  SRR4302540.8955329/94‑1 (MQ=60)
        GTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTG                                                                                      >  SRR4302540.5954232/1‑94 (MQ=60)
         TGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGC                                                                                     >  SRR4302540.4676147/1‑94 (MQ=60)
           CAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAG                                                                                   >  SRR4302540.8386768/1‑94 (MQ=60)
           CAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAG                                                                                   <  SRR4302540.971645/94‑1 (MQ=60)
            AGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGC                                                                                  >  SRR4302540.4049132/1‑94 (MQ=60)
             GCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCC                                                                                 >  SRR4302540.2424885/1‑94 (MQ=60)
              CGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCA                                                                                >  SRR4302540.9029938/1‑94 (MQ=60)
              CGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCA                                                                                >  SRR4302540.4898474/1‑94 (MQ=60)
               GCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCAT                                                                               >  SRR4302540.9297261/1‑94 (MQ=60)
               GCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCAT                                                                               <  SRR4302540.7488673/94‑1 (MQ=60)
                CGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATT                                                                              >  SRR4302540.4194296/1‑94 (MQ=60)
                 GCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTG                                                                             >  SRR4302540.4348239/1‑94 (MQ=60)
                  CCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGT                                                                            <  SRR4302540.1416245/94‑1 (MQ=60)
                  CCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGT                                                                            <  SRR4302540.7115836/94‑1 (MQ=60)
                  CCCGGGAACTGGGGCTAAAAATGATCATGGGCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGT                                                                            <  SRR4302540.1416244/94‑1 (MQ=60)
                   CCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCCGCCATTGTG                                                                           >  SRR4302540.5869973/1‑94 (MQ=60)
                   CCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTG                                                                           >  SRR4302540.6567247/1‑94 (MQ=60)
                   CCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTG                                                                           >  SRR4302540.4704247/1‑94 (MQ=60)
                   CCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTG                                                                           >  SRR4302540.2038961/1‑94 (MQ=60)
                    CGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGG                                                                          >  SRR4302540.5984033/1‑94 (MQ=60)
                    CGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGG                                                                          >  SRR4302540.2432745/1‑94 (MQ=60)
                    CGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGG                                                                          >  SRR4302540.2054472/1‑94 (MQ=60)
                    CGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGG                                                                          <  SRR4302540.6628442/94‑1 (MQ=60)
                     GGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGG                                                                         >  SRR4302540.6157851/1‑94 (MQ=60)
                     GGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGG                                                                         >  SRR4302540.4122316/1‑94 (MQ=60)
                     GGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGG                                                                         <  SRR4302540.6995289/94‑1 (MQ=60)
                     GGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGG                                                                         >  SRR4302540.1150492/1‑94 (MQ=60)
                     GGGAACTGAGTCTAAAAATGATCATTGTACCCGGTAGCCAGCTTACGCAACTGCCTCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGG                                                                         <  SRR4302540.7093964/94‑1 (MQ=60)
                      GGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGC                                                                        >  SRR4302540.4961314/1‑94 (MQ=60)
                      GGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGC                                                                        >  SRR4302540.7208631/1‑94 (MQ=60)
                       GAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCA                                                                       >  SRR4302540.929554/1‑94 (MQ=60)
                        AACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAA                                                                      >  SRR4302540.8758900/1‑94 (MQ=60)
                         ACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAT                                                                     <  SRR4302540.2561120/94‑1 (MQ=60)
                         ACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAAT                                                                     <  SRR4302540.5744992/94‑1 (MQ=60)
                          CTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATT                                                                    >  SRR4302540.9258884/1‑94 (MQ=60)
                          CTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATT                                                                    <  SRR4302540.6132412/94‑1 (MQ=60)
                          CTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATT                                                                    >  SRR4302540.552474/1‑94 (MQ=60)
                           TGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTT                                                                   >  SRR4302540.3556639/1‑94 (MQ=60)
                           TGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTT                                                                   >  SRR4302540.2825161/1‑94 (MQ=60)
                           TGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTT                                                                   <  SRR4302540.4419873/94‑1 (MQ=60)
                            GGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTA                                                                  >  SRR4302540.6449/1‑94 (MQ=60)
                            GGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTA                                                                  >  SRR4302540.5633524/1‑94 (MQ=60)
                            GGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTA                                                                  >  SRR4302540.7962510/1‑94 (MQ=60)
                             GGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTAC                                                                 >  SRR4302540.8901615/1‑94 (MQ=60)
                              GGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACT                                                                <  SRR4302540.4920302/94‑1 (MQ=60)
                               GCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTT                                                               <  SRR4302540.8643810/94‑1 (MQ=60)
                               GCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTT                                                               >  SRR4302540.2765023/1‑94 (MQ=60)
                                CTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTG                                                              >  SRR4302540.4229648/1‑94 (MQ=60)
                                CTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTG                                                              >  SRR4302540.244841/1‑94 (MQ=60)
                                CTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTG                                                              <  SRR4302540.6219899/94‑1 (MQ=60)
                                    AAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAA                                                          <  SRR4302540.2413489/94‑1 (MQ=60)
                                    AAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAA                                                          <  SRR4302540.3672266/94‑1 (MQ=60)
                                    AAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAA                                                          >  SRR4302540.7230577/1‑94 (MQ=60)
                                     AGTGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAAC                                                         <  SRR4302540.7313130/94‑1 (MQ=60)
                                     AATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAAC                                                         <  SRR4302540.6080292/94‑1 (MQ=60)
                                     AATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAAC                                                         <  SRR4302540.3427021/94‑1 (MQ=60)
                                      ATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACG                                                        >  SRR4302540.749834/1‑94 (MQ=60)
                                      ATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACG                                                        >  SRR4302540.9419735/1‑94 (MQ=60)
                                      ATGATCATTGGCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACG                                                        <  SRR4302540.1422292/94‑1 (MQ=60)
                                       TGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGC                                                       >  SRR4302540.3877152/1‑94 (MQ=60)
                                        GATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCC                                                      <  SRR4302540.9002793/94‑1 (MQ=60)
                                        GATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCC                                                      >  SRR4302540.824452/1‑94 (MQ=60)
                                         ATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCT                                                     <  SRR4302540.8523454/94‑1 (MQ=60)
                                         ATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCT                                                     <  SRR4302540.6012875/94‑1 (MQ=60)
                                         ATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCT                                                     >  SRR4302540.2606428/1‑94 (MQ=60)
                                          TCATTGTCCTCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTT                                                    >  SRR4302540.432311/1‑94 (MQ=60)
                                          TCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTT                                                    >  SRR4302540.5133501/1‑94 (MQ=60)
                                          TCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTT                                                    <  SRR4302540.8758548/94‑1 (MQ=60)
                                          TCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTT                                                    >  SRR4302540.195784/1‑94 (MQ=60)
                                           CATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTC                                                   <  SRR4302540.6519694/94‑1 (MQ=60)
                                           CATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTC                                                   <  SRR4302540.5063866/94‑1 (MQ=60)
                                             TTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGA                                                 <  SRR4302540.6808606/94‑1 (MQ=60)
                                             TTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACATGATAACGCCTTCGA                                                 >  SRR4302540.6500575/1‑94 (MQ=60)
                                              TGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAA                                                >  SRR4302540.2457208/1‑94 (MQ=60)
                                               GTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAG                                               >  SRR4302540.1241341/1‑94 (MQ=60)
                                                TCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGC                                              <  SRR4302540.1708/94‑1 (MQ=60)
                                                 CCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCC                                             <  SRR4302540.8876484/94‑1 (MQ=60)
                                                  CCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCA                                            >  SRR4302540.2696431/1‑94 (MQ=60)
                                                   CCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAG                                           >  SRR4302540.2462879/1‑94 (MQ=60)
                                                     GGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCC                                         >  SRR4302540.906488/1‑94 (MQ=60)
                                                     GGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCC                                         >  SRR4302540.4570281/1‑94 (MQ=60)
                                                      GTAGCCGGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCT                                        <  SRR4302540.10088950/94‑1 (MQ=60)
                                                      GTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCT                                        >  SRR4302540.7659497/1‑94 (MQ=60)
                                                      GTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCT                                        >  SRR4302540.5790003/1‑94 (MQ=60)
                                                      GTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCT                                        <  SRR4302540.7583642/94‑1 (MQ=60)
                                                        AGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGC                                      <  SRR4302540.7608969/94‑1 (MQ=60)
                                                         GCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCG                                     >  SRR4302540.5249832/1‑94 (MQ=60)
                                                           CAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTA                                   >  SRR4302540.5171011/1‑94 (MQ=60)
                                                           CAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTA                                   >  SRR4302540.1479613/1‑94 (MQ=60)
                                                           CAGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTA                                   >  SRR4302540.9064145/1‑94 (MQ=60)
                                                            AGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAG                                  >  SRR4302540.1044446/1‑94 (MQ=60)
                                                            AGCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAG                                  >  SRR4302540.875501/1‑94 (MQ=60)
                                                             TCTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGC                                 <  SRR4302540.7854207/94‑1 (MQ=60)
                                                              CTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCG                                <  SRR4302540.6019000/94‑1 (MQ=60)
                                                              CTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCG                                <  SRR4302540.3799259/94‑1 (MQ=60)
                                                              CTTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCG                                <  SRR4302540.4960312/94‑1 (MQ=60)
                                                               TTTCGCAACTGCGCCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTGCGAAGCCAGCCTGCGTAGCGA                               <  SRR4302540.1887342/94‑1 (MQ=60)
                                                               TTTCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGA                               >  SRR4302540.4456553/1‑94 (MQ=60)
                                                                TTCGCAACTGCCGCCAGGACTGGATAGCTCCGAGTTTGCAGCCATTGTGGGCAATTTCCTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGAT                              <  SRR4302540.5357168/94‑1 (MQ=60)
                                                                TTCGCAACAGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAGCGCCTTCGAAGCCAGCCTGCGTAGCGAT                              <  SRR4302540.9087841/94‑1 (MQ=60)
                                                                 TCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATG                             <  SRR4302540.9844608/94‑1 (MQ=60)
                                                                 TCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATG                             <  SRR4302540.3907589/94‑1 (MQ=60)
                                                                 TCGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATG                             <  SRR4302540.5857163/94‑1 (MQ=60)
                                                                  CGCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGA                            <  SRR4302540.3657731/94‑1 (MQ=60)
                                                                   GCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAA                           <  SRR4302540.2353348/94‑1 (MQ=60)
                                                                   GCAACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAA                           <  SRR4302540.299432/94‑1 (MQ=60)
                                                                     AACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGG                         >  SRR4302540.1605238/1‑94 (MQ=60)
                                                                      ACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGA                        >  SRR4302540.3692352/1‑94 (MQ=60)
                                                                      ACTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGA                        <  SRR4302540.8590640/94‑1 (MQ=60)
                                                                       CTGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAA                       >  SRR4302540.811070/1‑94 (MQ=60)
                                                                        TGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAC                      >  SRR4302540.9015418/1‑94 (MQ=60)
                                                                        TGCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAA                      <  SRR4302540.5805596/94‑1 (MQ=60)
                                                                         GCCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAAC                     <  SRR4302540.1081786/94‑1 (MQ=60)
                                                                          CCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACA                    <  SRR4302540.263354/94‑1 (MQ=60)
                                                                          CCGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACA                    <  SRR4302540.1156874/94‑1 (MQ=60)
                                                                           CGCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGGAATTTACTTGATAACGCCTTCGCAGCCAGCCTGCGTcgagctcaaagaacaca                   >  SRR4302540.1279693/1‑77 (MQ=60)
                                                                            GCCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAG                  >  SRR4302540.7640393/1‑94 (MQ=60)
                                                                             CCAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGA                 >  SRR4302540.1449189/1‑94 (MQ=60)
                                                                              CAGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGAT                >  SRR4302540.1204006/1‑94 (MQ=60)
                                                                               AGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATC               >  SRR4302540.9680634/1‑94 (MQ=60)
                                                                               AGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATC               >  SRR4302540.3069101/1‑94 (MQ=60)
                                                                               AGGACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATC               <  SRR4302540.6229314/94‑1 (MQ=60)
                                                                               AGGACTGGATAGCGCCGAGATTACAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATC               <  SRR4302540.9062097/94‑1 (MQ=60)
                                                                                 GACTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGT             >  SRR4302540.7693048/1‑94 (MQ=60)
                                                                                   CTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTG           <  SRR4302540.7425036/94‑1 (MQ=60)
                                                                                   CTGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTG           >  SRR4302540.6563531/1‑94 (MQ=60)
                                                                                    TGGATAGCGCCGAGTTTGCAGCCATTGTGTGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGA          <  SRR4302540.4165668/94‑1 (MQ=60)
                                                                                    TGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGA          <  SRR4302540.3728613/94‑1 (MQ=60)
                                                                                    TGGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGA          <  SRR4302540.6061902/94‑1 (MQ=60)
                                                                                     GGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAA         >  SRR4302540.961265/1‑94 (MQ=60)
                                                                                     GGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAA         <  SRR4302540.9707096/94‑1 (MQ=60)
                                                                                     GGATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAA         >  SRR4302540.8774051/1‑94 (MQ=60)
                                                                                      GATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAAT        >  SRR4302540.1632558/1‑94 (MQ=60)
                                                                                      GATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAAT        <  SRR4302540.5456583/94‑1 (MQ=60)
                                                                                      GATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAAT        <  SRR4302540.131723/94‑1 (MQ=60)
                                                                                       ATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATT       <  SRR4302540.606939/94‑1 (MQ=60)
                                                                                       ATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATT       >  SRR4302540.5304096/1‑94 (MQ=60)
                                                                                       ATAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATT       <  SRR4302540.4529913/94‑1 (MQ=60)
                                                                                        TAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTT      >  SRR4302540.6068974/1‑94 (MQ=60)
                                                                                        TAGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTA      <  SRR4302540.1235449/94‑1 (MQ=60)
                                                                                         AGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGTCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGACTTAT     >  SRR4302540.8030761/1‑94 (MQ=60)
                                                                                         AGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAaattggttaaatat     >  SRR4302540.9949039/1‑80 (MQ=60)
                                                                                         AGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTAT     >  SRR4302540.9605583/1‑94 (MQ=60)
                                                                                         AGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTAT     >  SRR4302540.8030762/1‑94 (MQ=60)
                                                                                         AGCGCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTAT     <  SRR4302540.6506519/94‑1 (MQ=60)
                                                                                         AGCGCCGAGTGTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTAT     <  SRR4302540.4128716/94‑1 (MQ=60)
                                                                                            GCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCC  >  SRR4302540.1782040/1‑94 (MQ=60)
                                                                                            GCCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCC  <  SRR4302540.9821393/94‑1 (MQ=60)
                                                                                            |                                                                                             
TTGGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAGCTTTCGCAACTGCCGCCAGGACTGGATAGCACCGAGTTTGCAGCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCC  >  NC_000913/652656‑652841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑