Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,236 T→C L357S (TTG→TCG)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,2360TC99.6% 812.6 / NA 250L357S (TTG→TCG) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (170/79);  total (171/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG  >  NC_000913/704148‑704328
                                                                                        |                                                                                            
tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                          <  1:7366439/93‑1 (MQ=255)
tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                          >  1:9466474/1‑93 (MQ=255)
  taACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAt                                                                                        <  1:6079203/93‑1 (MQ=255)
   aaCCTGCCGGCCGCCAGCCAGAATGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                       <  2:793026/93‑1 (MQ=255)
   aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                       <  1:4972436/93‑1 (MQ=255)
    aCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATcc                                                                                      >  2:3132654/1‑93 (MQ=255)
       tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCa                                                                                   <  1:9031502/93‑1 (MQ=255)
        gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                                  <  2:8474018/93‑1 (MQ=255)
        gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                                  <  2:6540557/93‑1 (MQ=255)
            gccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCTGTTATCCGCATGTTc                                                                              >  1:5710968/1‑93 (MQ=255)
            gccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTc                                                                              >  2:3268646/1‑93 (MQ=255)
              cgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCaa                                                                            >  1:9625337/1‑93 (MQ=255)
                    ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                      >  1:5560088/1‑93 (MQ=255)
                    ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                      >  2:9172959/1‑93 (MQ=255)
                    ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGG‑TATCCGCATGTTCAACCTGaaa                                                                     >  1:3486856/1‑93 (MQ=255)
                     cagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                     >  1:493151/1‑93 (MQ=255)
                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                    >  2:4164497/1‑93 (MQ=255)
                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                    >  1:1740287/1‑93 (MQ=255)
                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                    >  2:215425/1‑93 (MQ=255)
                        aaCGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACg                                                                  >  1:2914886/1‑93 (MQ=255)
                          cGTGTGGATGCTGCTGGTGATGGGCGTTGTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                <  1:6728690/93‑1 (MQ=255)
                          cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                <  2:1802054/93‑1 (MQ=255)
                          cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                >  2:7001946/1‑93 (MQ=255)
                            tCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCgg                                                              >  1:8699825/1‑93 (MQ=255)
                            tCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCgg                                                              >  1:2125013/1‑93 (MQ=255)
                              tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGt                                                            <  1:9200216/93‑1 (MQ=255)
                               ggATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                           <  2:5648646/93‑1 (MQ=255)
                                 aTGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGt                                                         >  1:4509207/1‑93 (MQ=255)
                                  tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                        >  1:1485551/1‑93 (MQ=255)
                                  tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                        >  2:7350228/1‑93 (MQ=255)
                                    ctgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGaa                                                      <  2:6700138/93‑1 (MQ=255)
                                     tgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                     >  2:5304962/1‑93 (MQ=255)
                                       ctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGAt                                                   >  1:6194686/1‑93 (MQ=255)
                                       ctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGAt                                                   >  1:4208126/1‑93 (MQ=255)
                                        tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                                  >  1:1172580/1‑93 (MQ=255)
                                        tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                                  >  1:2060094/1‑93 (MQ=255)
                                        tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                                  >  1:8602040/1‑93 (MQ=255)
                                         ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGACGATaa                                                 >  2:3961598/1‑93 (MQ=255)
                                         ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaa                                                 <  1:3132654/93‑1 (MQ=255)
                                          gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaaa                                                <  1:8087312/93‑1 (MQ=255)
                                          gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaaa                                                >  2:1743896/1‑93 (MQ=255)
                                             aTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaa                                             <  1:6562298/93‑1 (MQ=255)
                                              tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAacgaag                                            >  2:87166/1‑93 (MQ=255)
                                               gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                           >  1:8279175/1‑93 (MQ=255)
                                               gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                           >  1:7719916/1‑93 (MQ=255)
                                                ggCGTTATCTTCTTCGCTATCTAATTCGTGGTGTTCAGATCGGTTATCCGCATGTTCAACCTGAAAACGACGCGTCGTGAAGATAGAgacgac                                          >  2:4729351/1‑93 (MQ=255)
                                                   gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgag                                       >  1:4432973/1‑93 (MQ=255)
                                                   gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgag                                       >  2:828547/1‑93 (MQ=255)
                                                   gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgag                                       <  2:8893989/93‑1 (MQ=255)
                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                      >  1:1097324/1‑93 (MQ=255)
                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                      >  2:1225624/1‑93 (MQ=255)
                                                     tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                     >  1:9570531/1‑93 (MQ=255)
                                                     tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                     <  2:4554541/93‑1 (MQ=255)
                                                      aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAtg                                    >  2:2277439/1‑92 (MQ=255)
                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                   >  2:9675928/1‑93 (MQ=255)
                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                   >  2:6245304/1‑93 (MQ=255)
                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                   >  1:3816749/1‑93 (MQ=255)
                                                        tttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGt                                  <  1:3979568/92‑1 (MQ=255)
                                                         ttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGtt                                 <  1:5804372/93‑1 (MQ=255)
                                                          tcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGttt                                >  2:1923849/1‑92 (MQ=255)
                                                          tcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTa                                >  2:1037287/1‑93 (MQ=255)
                                                          tcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTa                                >  2:1590106/1‑93 (MQ=255)
                                                           cttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                               >  1:1442869/1‑93 (MQ=255)
                                                            ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAACACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACt                              >  1:5330970/1‑93 (MQ=255)
                                                            ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACt                              <  1:7273727/93‑1 (MQ=255)
                                                              cGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTga                            <  1:3405850/93‑1 (MQ=255)
                                                              cGCTATCTACTTCGTGGTGTTCAGCTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTga                            >  2:5856312/1‑93 (MQ=255)
                                                               gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                           >  2:7754359/1‑93 (MQ=255)
                                                               gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                           >  2:2190983/1‑93 (MQ=255)
                                                               gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                           >  1:523680/1‑93 (MQ=255)
                                                                 tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                         >  2:2396981/1‑93 (MQ=255)
                                                                 tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                         >  2:3521027/1‑93 (MQ=255)
                                                                 tATCTACGTCGTGGGGTTGAGTTCGGTTATCCGCATGTTCAACCTGAAAGCGCCGGGTCGTGTAGATAAAGAAGACGAGATCGTTACTgaaga                         >  2:4894299/1‑93 (MQ=255)
                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                        >  1:4772200/1‑93 (MQ=255)
                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                        >  2:5356443/1‑93 (MQ=255)
                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                        <  1:9259150/93‑1 (MQ=255)
                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                        <  2:3803922/93‑1 (MQ=255)
                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                        >  2:248277/1‑93 (MQ=255)
                                                                   tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAATACTCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                       >  2:2618428/1‑93 (MQ=255)
                                                                   tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                       >  2:6049520/1‑93 (MQ=255)
                                                                     tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGcc                     >  2:5982926/1‑93 (MQ=255)
                                                                       cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                   <  1:9747666/93‑1 (MQ=255)
                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                  <  2:4355634/93‑1 (MQ=255)
                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                  >  2:9074592/1‑93 (MQ=255)
                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                  <  2:8096155/93‑1 (MQ=255)
                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                  >  1:9845348/1‑93 (MQ=255)
                                                                          cGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg                <  2:7552870/93‑1 (MQ=255)
                                                                          cGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg                >  2:1998687/1‑93 (MQ=255)
                                                                            tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCa              >  2:6977312/1‑93 (MQ=255)
                                                                             ggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa             >  1:2579591/1‑93 (MQ=255)
                                                                             ggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa             <  1:2516094/93‑1 (MQ=255)
                                                                             ggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa             <  1:7487102/93‑1 (MQ=255)
                                                                              gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCacc            >  2:3361836/1‑91 (MQ=255)
                                                                               tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca           >  1:1759172/1‑93 (MQ=255)
                                                                                gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAacac          >  2:2310452/1‑93 (MQ=255)
                                                                                gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAacac          >  1:7044454/1‑93 (MQ=255)
                                                                                gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAacac          >  2:1618830/1‑93 (MQ=255)
                                                                                 ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt         <  1:3832196/93‑1 (MQ=255)
                                                                                   cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga       <  2:3640515/93‑1 (MQ=255)
                                                                                   cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga       <  1:1956231/93‑1 (MQ=255)
                                                                                        |                                                                                            
TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG  >  NC_000913/704148‑704328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704147‑704329
                                                                                         |                                                                                             
GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTT                                                                                           >  SRR4302540.8637467/1‑94 (MQ=60)
GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTT                                                                                           >  SRR4302540.9578773/1‑94 (MQ=60)
 TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTA                                                                                          <  SRR4302540.7448567/94‑1 (MQ=60)
  ATAACCTGCCGGCCGCCAGCCAGATCGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAT                                                                                         <  SRR4302540.7425669/94‑1 (MQ=60)
   TAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATC                                                                                        <  SRR4302540.6146801/94‑1 (MQ=60)
    AACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCC                                                                                       <  SRR4302540.5026243/94‑1 (MQ=60)
        ggccaGCCGCCAGCCAGCACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302540.9139888/89‑1 (MQ=60)
        TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302540.9139887/94‑1 (MQ=60)
        TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302540.6448310/94‑1 (MQ=60)
        TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302540.4863821/94‑1 (MQ=60)
          CCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGT                                                                                 >  SRR4302540.2925236/1‑94 (MQ=60)
           CGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTT                                                                                >  SRR4302540.3873254/1‑94 (MQ=60)
           CGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTT                                                                                <  SRR4302540.7527945/94‑1 (MQ=60)
            GGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCTGTTATCCGCATGTTC                                                                               >  SRR4302540.5773836/1‑94 (MQ=60)
            GGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTC                                                                               >  SRR4302540.9024081/1‑94 (MQ=60)
            GGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTC                                                                               >  SRR4302540.5773828/1‑94 (MQ=60)
              CCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAA                                                                             >  SRR4302540.9738772/1‑94 (MQ=60)
               CGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAC                                                                            >  SRR4302540.2123389/1‑94 (MQ=60)
               CGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAC                                                                            >  SRR4302540.5803033/1‑94 (MQ=60)
               CGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGAGTTCAGTTCGGTTATCCGCATGTTCAAC                                                                            >  SRR4302540.8028796/1‑94 (MQ=60)
                GCCAGCCAGAACGTCTGGATGCTGCTGGCGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACC                                                                           >  SRR4302540.9482786/1‑94 (MQ=60)
                 CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          <  SRR4302540.1681776/94‑1 (MQ=60)
                 CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          >  SRR4302540.3450486/1‑94 (MQ=60)
                    agCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTGCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAA                                                                       <  SRR4302540.5987150/92‑1 (MQ=60)
                    GCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAA                                                                       >  SRR4302540.5621322/1‑94 (MQ=60)
                    GCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGG‑TATCCGCATGTTCAACCTGAAA                                                                      >  SRR4302540.3524826/1‑94 (MQ=60)
                     CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      >  SRR4302540.498437/1‑94 (MQ=60)
                     CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      >  SRR4302540.340562/1‑94 (MQ=60)
                     CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      <  SRR4302540.7635926/94‑1 (MQ=60)
                      CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     >  SRR4302540.1759111/1‑94 (MQ=60)
                      CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTAAACCTGAAAA                                                                     >  SRR4302540.1759112/1‑94 (MQ=60)
                        GAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACG                                                                   >  SRR4302540.2946776/1‑94 (MQ=60)
                           CGTGTGGATGCTGCTGGTGATGGGCGTTGTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCG                                                                <  SRR4302540.6802982/94‑1 (MQ=60)
                           CGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCG                                                                >  SRR4302540.5255552/1‑94 (MQ=60)
                           CGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGGTCGGTTATCCGCATGTTCAACCTGCAAACGCCG                                                                >  SRR4302540.5255553/1‑94 (MQ=60)
                            GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               >  SRR4302540.2147814/1‑94 (MQ=60)
                            GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               >  SRR4302540.8800813/1‑94 (MQ=60)
                               TGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTC                                                            <  SRR4302540.9310594/94‑1 (MQ=60)
                               TGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTC                                                            <  SRR4302540.5350325/94‑1 (MQ=60)
                                 GATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGT                                                          >  SRR4302540.4558069/1‑94 (MQ=60)
                                  ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAACACGCCGGGTCGTG                                                         >  SRR4302540.1501858/1‑94 (MQ=60)
                                  ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTG                                                         >  SRR4302540.1501860/1‑94 (MQ=60)
                                    GCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAA                                                       >  SRR4302540.2003451/1‑94 (MQ=60)
                                     CTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAG                                                      >  SRR4302540.9695049/1‑94 (MQ=60)
                                       GCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGAT                                                    >  SRR4302540.6263656/1‑94 (MQ=60)
                                       GCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGAT                                                    >  SRR4302540.4877749/1‑94 (MQ=60)
                                       GCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGAT                                                    >  SRR4302540.4253935/1‑94 (MQ=60)
                                        CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   <  SRR4302540.1955984/94‑1 (MQ=60)
                                        CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302540.8702011/1‑94 (MQ=60)
                                        CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302540.1184713/1‑94 (MQ=60)
                                        CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302540.2082266/1‑94 (MQ=60)
                                         TGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAA                                                  >  SRR4302540.162570/1‑94 (MQ=60)
                                          GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 <  SRR4302540.3166837/94‑1 (MQ=60)
                                           GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                <  SRR4302540.8181183/94‑1 (MQ=60)
                                           GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                >  SRR4302540.9724020/1‑94 (MQ=60)
                                            TGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGA                                               >  SRR4302540.418025/1‑94 (MQ=60)
                                              ATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG                                             <  SRR4302540.6635036/94‑1 (MQ=60)
                                               TGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGC                                            >  SRR4302540.3605780/1‑94 (MQ=60)
                                               TGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGA                                            >  SRR4302540.8375336/1‑94 (MQ=60)
                                               TGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGA                                            >  SRR4302540.7809390/1‑94 (MQ=60)
                                               TGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGA                                            <  SRR4302540.5590038/94‑1 (MQ=60)
                                                GGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           <  SRR4302540.3879298/94‑1 (MQ=60)
                                                GGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           <  SRR4302540.8878240/94‑1 (MQ=60)
                                                GGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           >  SRR4302540.4519994/1‑94 (MQ=60)
                                                  GCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGA                                         <  SRR4302540.1258823/94‑1 (MQ=60)
                                                   CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        >  SRR4302540.4481003/1‑94 (MQ=60)
                                                    GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGC                                       >  SRR4302540.1281430/1‑94 (MQ=60)
                                                    GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302540.1108622/1‑94 (MQ=60)
                                                    GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302540.432917/1‑94 (MQ=60)
                                                    GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302540.5744581/1‑94 (MQ=60)
                                                    GTTATCTTCTTCGCTATCTACTTCGTGGTGTACAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302540.9589218/1‑94 (MQ=60)
                                                     TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      <  SRR4302540.2141324/94‑1 (MQ=60)
                                                     TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      >  SRR4302540.9289883/1‑94 (MQ=60)
                                                     TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      >  SRR4302540.9683599/1‑94 (MQ=60)
                                                      TATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATC                                     <  SRR4302540.6362240/94‑1 (MQ=60)
                                                       ATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCG                                    >  SRR4302540.3858303/1‑94 (MQ=60)
                                                        TCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGT                                   >  SRR4302540.1922369/1‑94 (MQ=60)
                                                         TTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  <  SRR4302540.4022436/94‑1 (MQ=60)
                                                         CTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  <  SRR4302540.7337049/94‑1 (MQ=60)
                                                         CTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  >  SRR4302540.9768319/1‑94 (MQ=60)
                                                          TTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGTTA                                 <  SRR4302540.5868508/94‑1 (MQ=60)
                                                          TTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTA                                 <  SRR4302540.2731267/94‑1 (MQ=60)
                                                          TTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTA                                 <  SRR4302540.4500524/94‑1 (MQ=60)
                                                          TTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTA                                 >  SRR4302540.4996576/1‑94 (MQ=60)
                                                           TCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAC                                >  SRR4302540.7027398/1‑94 (MQ=60)
                                                           TCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAC                                >  SRR4302540.1458543/1‑94 (MQ=60)
                                                            CTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAACACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACT                               >  SRR4302540.5389376/1‑94 (MQ=60)
                                                             TTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTG                              >  SRR4302540.3820829/1‑94 (MQ=60)
                                                             TTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTG                              <  SRR4302540.7354891/94‑1 (MQ=60)
                                                              TCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGA                             >  SRR4302540.3949798/1‑94 (MQ=60)
                                                               CGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAA                            >  SRR4302540.529324/1‑94 (MQ=60)
                                                               CGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAA                            <  SRR4302540.3442964/94‑1 (MQ=60)
                                                               CGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAA                            <  SRR4302540.6164615/94‑1 (MQ=60)
                                                                GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           <  SRR4302540.2379482/94‑1 (MQ=60)
                                                                 CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          >  SRR4302540.10044556/1‑94 (MQ=60)
                                                                 CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          >  SRR4302540.7151400/1‑94 (MQ=60)
                                                                  TATCTCCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         <  SRR4302540.8090314/94‑1 (MQ=60)
                                                                  TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         >  SRR4302540.4823181/1‑94 (MQ=60)
                                                                  TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         <  SRR4302540.8234341/94‑1 (MQ=60)
                                                                   ATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAG                        >  SRR4302540.7539055/1‑94 (MQ=60)
                                                                   ATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAG                        >  SRR4302540.3432991/1‑94 (MQ=60)
                                                                   ATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAG                        <  SRR4302540.9369926/94‑1 (MQ=60)
                                                                     CTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCC                      <  SRR4302540.6653740/94‑1 (MQ=60)
                                                                     CTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCC                      >  SRR4302540.9371313/1‑94 (MQ=60)
                                                                        CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   >  SRR4302540.9960437/1‑94 (MQ=60)
                                                                        CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   <  SRR4302540.9862060/94‑1 (MQ=60)
                                                                        CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   >  SRR4302540.9960436/1‑94 (MQ=60)
                                                                           CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGC                <  SRR4302540.1154958/94‑1 (MQ=60)
                                                                            GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCA               >  SRR4302540.2574991/1‑94 (MQ=60)
                                                                            GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCA               >  SRR4302540.7042078/1‑94 (MQ=60)
                                                                             TGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAA              >  SRR4302540.2607403/1‑94 (MQ=60)
                                                                              GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAAC             <  SRR4302540.2543273/94‑1 (MQ=60)
                                                                              GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAAC             <  SRR4302540.24077/94‑1 (MQ=60)
                                                                              GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAAC             <  SRR4302540.7570679/94‑1 (MQ=60)
                                                                               GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            >  SRR4302540.1778244/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATACAGAAGACGAGATCGTTACTGCAGAAGCCAACAGCAACAC           >  SRR4302540.4130585/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           <  SRR4302540.9911383/94‑1 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           >  SRR4302540.10060235/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           >  SRR4302540.8800276/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           >  SRR4302540.8443093/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           >  SRR4302540.7122783/1‑94 (MQ=60)
                                                                                TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           <  SRR4302540.6086041/94‑1 (MQ=60)
                                                                                 GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACT          <  SRR4302540.9355442/94‑1 (MQ=60)
                                                                                  TTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTG         <  SRR4302540.3873900/94‑1 (MQ=60)
                                                                                   TCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGA        >  SRR4302540.4763486/1‑94 (MQ=60)
                                                                                    CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAA       <  SRR4302540.3046462/94‑1 (MQ=60)
                                                                                    CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAA       <  SRR4302540.1977501/94‑1 (MQ=60)
                                                                                       TTCGGTTATCCGCATGTTCAACCTGAAAGCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAA    <  SRR4302540.6308393/94‑1 (MQ=60)
                                                                                       TTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAA    <  SRR4302540.6401854/94‑1 (MQ=60)
                                                                                       TTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAA    <  SRR4302540.5565834/94‑1 (MQ=60)
                                                                                        TCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG   <  SRR4302540.358451/94‑1 (MQ=60)
                                                                                         TGGTTATCAGCATGTTCAACCTGAAAACGCCGAGTGTGGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302540.5351328/94‑1 (MQ=60)
                                                                                         CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCGACAGCAACACTGAAGAAGG  >  SRR4302540.9762179/1‑94 (MQ=60)
                                                                                         CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302540.3512496/94‑1 (MQ=60)
                                                                                         |                                                                                             
GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704147‑704329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑