Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,236 T→C L357S (TTG→TCG)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,2360TC100.0% 714.6 / NA 220L357S (TTG→TCG) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (122/98);  total (122/98)

Only 100 of 227 total aligned reads displayed.

TGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704146‑704325
                                                                                          |                                                                                         
  tataACCTGCCGGCCGCCAGCGAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  1:1517769/93‑1 (MQ=255)
  tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  2:661233/93‑1 (MQ=255)
  tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  1:5772859/93‑1 (MQ=255)
   atatCCTGCGGGCTGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGCCGTTATCTTCTTCGCTATCTTCTTCGTGGTTTTCAGTTCGGTTa                                                                                      <  2:5528417/93‑1 (MQ=255)
   ataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTa                                                                                      <  1:3068195/93‑1 (MQ=255)
   ataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTa                                                                                      <  1:2206119/93‑1 (MQ=255)
     aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                    <  2:3158205/93‑1 (MQ=255)
          gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                               >  1:226631/1‑93 (MQ=255)
            cggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGt                                                                             <  1:2658063/93‑1 (MQ=255)
              gccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATGCGCATGTTc                                                                           >  1:861810/1‑93 (MQ=255)
                 gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAc                                                                        >  1:5172803/1‑93 (MQ=255)
                 gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAc                                                                        >  2:5720233/1‑93 (MQ=255)
                  ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       <  1:2787833/93‑1 (MQ=255)
                  ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       <  2:2902528/93‑1 (MQ=255)
                   cagccagAACGTCTGGGTGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCt                                                                      >  2:2884209/1‑93 (MQ=255)
                   cagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCt                                                                      <  2:2099790/93‑1 (MQ=255)
                    agccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTg                                                                     >  1:4694013/1‑93 (MQ=255)
                     gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                    <  1:4929813/93‑1 (MQ=255)
                      ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   <  1:2821929/93‑1 (MQ=255)
                      ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   <  2:3127154/93‑1 (MQ=255)
                      ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   >  2:257708/1‑93 (MQ=255)
                       cagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                  >  2:779035/1‑93 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:5577290/1‑93 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:5226277/1‑93 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  2:1869752/93‑1 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:1089810/1‑93 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:3241090/1‑93 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  2:3590603/93‑1 (MQ=255)
                        agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  2:5061336/1‑93 (MQ=255)
                         gAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                >  1:4882069/1‑93 (MQ=255)
                            cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                             <  2:3313614/93‑1 (MQ=255)
                             gTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCg                                                            >  1:2090953/1‑93 (MQ=255)
                                tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGt                                                         <  2:4096832/93‑1 (MQ=255)
                                 ggATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                        >  2:757389/1‑93 (MQ=255)
                                  gATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCg                                                       >  2:4856929/1‑93 (MQ=255)
                                    tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                     >  1:1154505/1‑93 (MQ=255)
                                    tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                     >  1:451989/1‑93 (MQ=255)
                                    tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                     >  1:2573434/1‑93 (MQ=255)
                                     gctgctGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    >  1:1617433/1‑93 (MQ=255)
                                     gctgctGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    <  1:2967179/93‑1 (MQ=255)
                                     gctgctGGTGATGGGCGTTACCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    <  2:586542/93‑1 (MQ=255)
                                      ctgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGaa                                                   >  2:3487726/1‑93 (MQ=255)
                                       tgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                  >  2:2969767/1‑93 (MQ=255)
                                       tgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                  >  2:5378226/1‑93 (MQ=255)
                                        gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGa                                                 >  2:5755841/1‑93 (MQ=255)
                                           ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaa                                              <  2:4615936/93‑1 (MQ=255)
                                            gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaaa                                             <  1:1472362/93‑1 (MQ=255)
                                            gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAAGCCGGGTCGTGAAGATaaa                                             >  1:3454904/1‑93 (MQ=255)
                                              gATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaaga                                           <  1:5429588/93‑1 (MQ=255)
                                                tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         <  2:5200948/93‑1 (MQ=255)
                                                tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         <  2:4769866/93‑1 (MQ=255)
                                                tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         >  1:5111977/1‑93 (MQ=255)
                                                  ggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAc                                       <  2:2202853/93‑1 (MQ=255)
                                                   gCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACg                                      <  2:4722141/93‑1 (MQ=255)
                                                    cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     >  1:4582153/1‑93 (MQ=255)
                                                    cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     <  1:1361442/93‑1 (MQ=255)
                                                    cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     >  1:6292720/1‑93 (MQ=255)
                                                    cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     <  1:664085/93‑1 (MQ=255)
                                                      ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   >  2:5522427/1‑93 (MQ=255)
                                                      ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   >  1:936760/1‑93 (MQ=255)
                                                      ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   >  2:4375259/1‑93 (MQ=255)
                                                       tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                  >  1:1768552/1‑93 (MQ=255)
                                                        ttcttcttctCCATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 <  2:4928615/92‑1 (MQ=255)
                                                        ttcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 <  1:3281517/92‑1 (MQ=255)
                                                         tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTTAAGATAAAGAAGACGAGATCg                                <  2:2754510/93‑1 (MQ=255)
                                                         tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                <  2:3859735/93‑1 (MQ=255)
                                                              ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACt                           >  2:2274740/1‑93 (MQ=255)
                                                               tcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTg                          <  2:775993/93‑1 (MQ=255)
                                                                 gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        <  2:6245858/93‑1 (MQ=255)
                                                                 gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        >  2:1374434/1‑93 (MQ=255)
                                                                 gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        <  1:1869775/93‑1 (MQ=255)
                                                                  cTATCTTCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       <  1:2310467/93‑1 (MQ=255)
                                                                  cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       >  1:2885735/1‑93 (MQ=255)
                                                                   tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                      <  2:1652771/93‑1 (MQ=255)
                                                                   tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                      <  1:3624985/93‑1 (MQ=255)
                                                                   tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                      >  2:3248400/1‑93 (MQ=255)
                                                                    aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGCAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  1:73322/1‑93 (MQ=255)
                                                                    aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     <  2:3300986/93‑1 (MQ=255)
                                                                    aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  2:2475919/1‑93 (MQ=255)
                                                                    aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  1:6077268/1‑93 (MQ=255)
                                                                      cTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                   <  2:1799156/93‑1 (MQ=255)
                                                                      cTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACAAGATCGTTACGAAAGAAGc                   <  2:6114103/93‑1 (MQ=255)
                                                                       tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGcc                  >  2:4453485/1‑93 (MQ=255)
                                                                       tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGcc                  >  1:3977251/1‑93 (MQ=255)
                                                                        aCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCa                 >  1:1569277/1‑93 (MQ=255)
                                                                        aCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCa                 >  1:4254485/1‑93 (MQ=255)
                                                                        aCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCa                 >  1:4064912/1‑93 (MQ=255)
                                                                          ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc               >  1:3873161/1‑93 (MQ=255)
                                                                           tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACa              <  2:5330511/93‑1 (MQ=255)
                                                                              tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCa           >  2:3971214/1‑93 (MQ=255)
                                                                               ggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa          >  1:1930225/1‑93 (MQ=255)
                                                                                gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         <  2:108802/93‑1 (MQ=255)
                                                                                gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         >  2:3779743/1‑93 (MQ=255)
                                                                                gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         <  2:3227564/93‑1 (MQ=255)
                                                                                 tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        >  2:5943907/1‑93 (MQ=255)
                                                                                 tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        <  2:2856350/93‑1 (MQ=255)
                                                                                   ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt      >  2:449596/1‑93 (MQ=255)
                                                                                     cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga    <  2:6089468/93‑1 (MQ=255)
                                                                                     cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga    <  2:2714388/93‑1 (MQ=255)
                                                                                       gTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaag  >  1:3480859/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704146‑704325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704144‑704329
                                                                                            |                                                                                             
GATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCG                                                                                              >  SRR4302533.4715125/1‑94 (MQ=60)
GATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCG                                                                                              >  SRR4302533.550695/1‑94 (MQ=60)
 ATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGG                                                                                             >  SRR4302533.5507787/1‑94 (MQ=60)
    TATAACCTGCCGGCCGCCAGCGAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTA                                                                                          <  SRR4302533.1526904/94‑1 (MQ=60)
    TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTA                                                                                          <  SRR4302533.5807721/94‑1 (MQ=60)
     ATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAT                                                                                         <  SRR4302533.2219723/94‑1 (MQ=60)
     ATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAT                                                                                         <  SRR4302533.3087036/94‑1 (MQ=60)
       AACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCC                                                                                       >  SRR4302533.2337145/1‑94 (MQ=60)
          CTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCA                                                                                    >  SRR4302533.5420037/1‑94 (MQ=60)
           TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   >  SRR4302533.2896486/1‑94 (MQ=60)
           TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   >  SRR4302533.228042/1‑94 (MQ=60)
           CGCCGGCGGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302533.875666/94‑1 (MQ=60)
             CCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGT                                                                                 <  SRR4302533.4617690/94‑1 (MQ=60)
              CGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTT                                                                                <  SRR4302533.2674393/94‑1 (MQ=60)
               GGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATGCGCATGTTC                                                                               >  SRR4302533.866859/1‑94 (MQ=60)
                GCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCA                                                                              <  SRR4302533.1626756/94‑1 (MQ=60)
                  CGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAC                                                                            >  SRR4302533.5204015/1‑94 (MQ=60)
                    CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          >  SRR4302533.1931733/1‑94 (MQ=60)
                    CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          <  SRR4302533.2804962/94‑1 (MQ=60)
                     CAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTG                                                                         >  SRR4302533.4722457/1‑94 (MQ=60)
                      AGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGA                                                                        >  SRR4302533.1401352/1‑94 (MQ=60)
                       GCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAA                                                                       <  SRR4302533.4959666/94‑1 (MQ=60)
                        CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      <  SRR4302533.3744827/94‑1 (MQ=60)
                        CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      <  SRR4302533.2839297/94‑1 (MQ=60)
                         CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     >  SRR4302533.5257725/1‑94 (MQ=60)
                         CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     >  SRR4302533.3261064/1‑94 (MQ=60)
                         CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     >  SRR4302533.5610995/1‑94 (MQ=60)
                         CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     >  SRR4302533.1096246/1‑94 (MQ=60)
                          AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    >  SRR4302533.4089109/1‑94 (MQ=60)
                          AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    >  SRR4302533.4911645/1‑94 (MQ=60)
                          AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    >  SRR4302533.1818361/1‑94 (MQ=60)
                          AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    <  SRR4302533.2291982/94‑1 (MQ=60)
                          AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    >  SRR4302533.3492100/1‑94 (MQ=60)
                            AACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGC                                                                  >  SRR4302533.382495/1‑94 (MQ=60)
                              CGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCG                                                                >  SRR4302533.2103832/1‑94 (MQ=60)
                              CGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCG                                                                >  SRR4302533.1506854/1‑94 (MQ=60)
                              CGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCG                                                                <  SRR4302533.698816/94‑1 (MQ=60)
                               GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               <  SRR4302533.3039020/94‑1 (MQ=60)
                               GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               >  SRR4302533.745344/1‑94 (MQ=60)
                               GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               <  SRR4302533.1158338/94‑1 (MQ=60)
                                   GGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCG                                                           >  SRR4302533.1340375/1‑94 (MQ=60)
                                     ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTG                                                         >  SRR4302533.454697/1‑94 (MQ=60)
                                     ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTG                                                         >  SRR4302533.1161427/1‑94 (MQ=60)
                                     ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTG                                                         >  SRR4302533.2589265/1‑94 (MQ=60)
                                      TGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGA                                                        >  SRR4302533.1627233/1‑94 (MQ=60)
                                      TGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGA                                                        <  SRR4302533.2227533/94‑1 (MQ=60)
                                       GCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAA                                                       <  SRR4302533.2985318/94‑1 (MQ=60)
                                           CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302533.6229457/1‑94 (MQ=60)
                                             GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 >  SRR4302533.2801478/1‑94 (MQ=60)
                                             GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 >  SRR4302533.2088865/1‑94 (MQ=60)
                                             GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 <  SRR4302533.3610383/94‑1 (MQ=60)
                                             GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 <  SRR4302533.3466743/94‑1 (MQ=60)
                                             GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAAGCCGGGTCGTGAAGATAAA                                                 >  SRR4302533.3476229/1‑94 (MQ=60)
                                              GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                <  SRR4302533.5828128/94‑1 (MQ=60)
                                              GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                <  SRR4302533.1481260/94‑1 (MQ=60)
                                              GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                <  SRR4302533.157568/94‑1 (MQ=60)
                                               TGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGA                                               >  SRR4302533.3131509/1‑94 (MQ=60)
                                               TGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGA                                               >  SRR4302533.258957/1‑94 (MQ=60)
                                                GATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAA                                              <  SRR4302533.5462288/94‑1 (MQ=60)
                                                 ATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG                                             >  SRR4302533.5142869/1‑94 (MQ=60)
                                                     GCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGA                                         >  SRR4302533.6330236/1‑94 (MQ=60)
                                                     GCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGA                                         >  SRR4302533.4609918/1‑94 (MQ=60)
                                                      CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302533.668005/94‑1 (MQ=60)
                                                      CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302533.834963/94‑1 (MQ=60)
                                                      CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302533.1369620/94‑1 (MQ=60)
                                                       GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302533.5242024/1‑94 (MQ=60)
                                                       GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302533.4836903/1‑94 (MQ=60)
                                                       GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302533.2833144/1‑94 (MQ=60)
                                                       GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302533.942265/1‑94 (MQ=60)
                                                       GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302533.2168913/1‑94 (MQ=60)
                                                        TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      <  SRR4302533.4481819/94‑1 (MQ=60)
                                                        TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      >  SRR4302533.1779321/1‑94 (MQ=60)
                                                          TTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCG                                    <  SRR4302533.3301777/94‑1 (MQ=60)
                                                          ATCTTCTTCGCTATCTACTTCGTGTTGTTAACTTCGGTTTTCCGCATGTTCAACCTAAAAACGCCctgtcatgaagacaaatacaaccacacat                                    >  SRR4302533.3509436/1‑65 (MQ=34)
                                                            CGTCTTCGCTATCTGCTTCGTGGTTTTCAGTCCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  <  SRR4302533.3981362/94‑1 (MQ=60)
                                                             TTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTA                                 >  SRR4302533.4272675/1‑94 (MQ=60)
                                                                 TCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGA                             >  SRR4302533.6341641/1‑94 (MQ=60)
                                                                 TCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGA                             >  SRR4302533.4946533/1‑94 (MQ=60)
                                                                   GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           >  SRR4302533.2903480/1‑94 (MQ=60)
                                                                   GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           <  SRR4302533.1881174/94‑1 (MQ=60)
                                                                    CTATCTTCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          <  SRR4302533.2324781/94‑1 (MQ=60)
                                                                    CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          <  SRR4302533.582760/94‑1 (MQ=60)
                                                                    CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          >  SRR4302533.1511023/1‑94 (MQ=60)
                                                                     TATCTGCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         <  SRR4302533.5802915/94‑1 (MQ=60)
                                                                     TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGCAGATAAAGAAGACGAGATCGTTACTGAAGAA                         >  SRR4302533.73793/1‑94 (MQ=60)
                                                                     TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         >  SRR4302533.6114025/1‑94 (MQ=60)
                                                                     TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         <  SRR4302533.2224983/94‑1 (MQ=60)
                                                                     TATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAA                         <  SRR4302533.3647290/94‑1 (MQ=60)
                                                                      ATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAG                        <  SRR4302533.2958026/94‑1 (MQ=60)
                                                                      ATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAG                        >  SRR4302533.1653961/1‑94 (MQ=60)
                                                                       TCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGC                       <  SRR4302533.2978558/94‑1 (MQ=60)
                                                                        CTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCC                      >  SRR4302533.4001467/1‑94 (MQ=60)
                                                                         TACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCA                     >  SRR4302533.5369320/1‑94 (MQ=60)
                                                                         TACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCA                     >  SRR4302533.4280140/1‑94 (MQ=60)
                                                                         TACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCA                     >  SRR4302533.4089656/1‑94 (MQ=60)
                                                                         TACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCA                     >  SRR4302533.1578726/1‑94 (MQ=60)
                                                                           CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   >  SRR4302533.3896801/1‑94 (MQ=60)
                                                                           CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   >  SRR4302533.5140254/1‑94 (MQ=60)
                                                                            TTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACA                  <  SRR4302533.1865117/94‑1 (MQ=60)
                                                                              CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGC                >  SRR4302533.4916024/1‑94 (MQ=60)
                                                                                TGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAA              >  SRR4302533.1941999/1‑94 (MQ=60)
                                                                                TGGTGTTAAGTTCGGTTNTCCGCATGTTCAACCTGAAAACGCCGGGTCNTGAAGATAAAGAAGACGAGATNNNTNCTGAAGAAGCNANNNGNAA              >  SRR4302533.4862348/1‑94 (MQ=60)
                                                                                  GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            <  SRR4302533.264239/94‑1 (MQ=60)
                                                                                    GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACT          <  SRR4302533.4343566/94‑1 (MQ=60)
                                                                                     TTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTG         >  SRR4302533.456854/1‑94 (MQ=60)
                                                                                       CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCATCAGCAACACTGAA       <  SRR4302533.3427363/94‑1 (MQ=60)
                                                                                       CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAA       <  SRR4302533.3279628/94‑1 (MQ=60)
                                                                                        AGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG      >  SRR4302533.3502332/1‑94 (MQ=60)
                                                                                         GTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGA     <  SRR4302533.5193105/94‑1 (MQ=60)
                                                                                           TCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG   <  SRR4302533.1449552/94‑1 (MQ=60)
                                                                                            CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302533.1336986/94‑1 (MQ=60)
                                                                                            |                                                                                             
GATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704144‑704329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑