Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,405 C→T A159A (GCC→GCT)  sucA → 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,4050CT100.0% 888.8 / NA 242A159A (GCC→GCT) sucA2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (102/140);  total (102/140)

Only 100 of 247 total aligned reads displayed.

TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA  >  NC_000913/758315‑758494
                                                                                          |                                                                                         
  tGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCa                                                                                       <  1:1473566/93‑1 (MQ=255)
  tGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCa                                                                                       >  2:1932389/1‑93 (MQ=255)
   gACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCaa                                                                                      >  1:8663814/1‑93 (MQ=255)
    aCCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAg                                                                                     <  2:4975004/93‑1 (MQ=255)
        aaGCAGACTTCAAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCaaa                                                                                 >  2:8207727/1‑93 (MQ=255)
         aGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAAc                                                                                <  1:4949772/93‑1 (MQ=255)
          gCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAAcc                                                                               <  1:4878977/93‑1 (MQ=255)
            aGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTa                                                                             <  1:9931634/93‑1 (MQ=255)
             gaattCCAGGAAGCCTTCAAAGTCGGTTCATAAGCCAGCGACAAAGAAACCATAAAACTCGACGAGCTGCTGAAAGCTCTCAAGCAAACCTAc                                                                            <  1:8926532/90‑1 (MQ=255)
             gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAc                                                                            <  2:8511296/93‑1 (MQ=255)
              aCTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACt                                                                           <  1:3188748/93‑1 (MQ=255)
              aCTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACt                                                                           >  2:3206710/1‑93 (MQ=255)
                ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGc                                                                         >  1:188308/1‑93 (MQ=255)
                 tCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAACTGCTGGAAGCTCTCAAGCAAACCTACTGCg                                                                        <  2:4181652/93‑1 (MQ=255)
                  ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCgg                                                                       >  2:7716673/1‑93 (MQ=255)
                   cAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGc                                                                      <  2:3013117/93‑1 (MQ=255)
                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     <  2:2548244/93‑1 (MQ=255)
                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     >  1:4084975/1‑93 (MQ=255)
                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     >  1:8047531/1‑93 (MQ=255)
                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     <  2:4642814/93‑1 (MQ=255)
                     ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGccc                                                                    >  1:2579229/1‑93 (MQ=255)
                     ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGccc                                                                    >  2:4314811/1‑93 (MQ=255)
                         aCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAtt                                                                <  2:8282823/93‑1 (MQ=255)
                          ccTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTg                                                               <  1:4489392/93‑1 (MQ=255)
                          ccTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTg                                                               >  1:9271107/1‑93 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCTAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGGCCGATTgg                                                              >  2:9557222/1‑93 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGGAAACCTACTGCGGCCCGATTgg                                                              <  1:4385989/93‑1 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              <  1:9502744/93‑1 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              >  1:9399573/1‑93 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              >  1:9221150/1‑93 (MQ=255)
                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              <  1:6217328/93‑1 (MQ=255)
                            ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                             >  2:3832325/1‑93 (MQ=255)
                            ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                             >  2:6918423/1‑93 (MQ=255)
                            ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                             >  1:4520556/1‑93 (MQ=255)
                            ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                             >  1:2110896/1‑93 (MQ=255)
                             tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                            >  2:558288/1‑93 (MQ=255)
                             tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                            >  1:6943888/1‑93 (MQ=255)
                             tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                            >  2:1880338/1‑93 (MQ=255)
                              cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGc                                                           <  1:8754001/93‑1 (MQ=255)
                              cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGc                                                           <  1:8472066/93‑1 (MQ=255)
                               aaCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGcc                                                          <  1:62713/93‑1 (MQ=255)
                                 cgtcgtTTCATTGGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGa                                                        <  1:5530294/93‑1 (MQ=255)
                                      gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                                   >  2:1276140/1‑93 (MQ=255)
                                      gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                                   >  2:8973404/1‑93 (MQ=255)
                                        tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATg                                                 >  1:1555395/1‑93 (MQ=255)
                                         cATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                                >  2:8692070/1‑93 (MQ=255)
                                         cATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                                <  1:2480025/93‑1 (MQ=255)
                                         cATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                                >  1:664316/1‑93 (MQ=255)
                                            ttGCCAGCGGCAAAGAAACCATGAACCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                             <  1:7869946/93‑1 (MQ=255)
                                            ttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                             <  1:3409578/93‑1 (MQ=255)
                                            ttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                             <  1:9033768/93‑1 (MQ=255)
                                            ttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                             <  2:2684648/93‑1 (MQ=255)
                                               ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTa                                          >  2:3904350/1‑93 (MQ=255)
                                                  gCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                                       <  1:9045645/93‑1 (MQ=255)
                                                  gCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                                       >  2:4750026/1‑93 (MQ=255)
                                                  gCGGCAAAGAAACCATGAAACTCGGAGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                                       >  2:6062725/1‑93 (MQ=255)
                                                   cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAg                                      <  2:7948919/93‑1 (MQ=255)
                                                   cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAg                                      <  1:3570984/93‑1 (MQ=255)
                                                    ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGc                                     <  1:9545714/93‑1 (MQ=255)
                                                     gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGACGAGTATATGCACATTACCAGCa                                    <  1:2283029/93‑1 (MQ=255)
                                                       aaaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                                  >  1:2536180/1‑93 (MQ=255)
                                                        aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCg                                 >  1:625673/1‑93 (MQ=255)
                                                         aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                                >  1:5115403/1‑93 (MQ=255)
                                                         aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                                <  1:7336793/93‑1 (MQ=255)
                                                          gAAACCATGAAACTCGGCGAGCTGCTGTAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               <  1:108582/93‑1 (MQ=255)
                                                          gAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               <  1:2654554/93‑1 (MQ=255)
                                                          gAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               <  1:1185309/93‑1 (MQ=255)
                                                            aaCCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaaga                             >  2:2217348/1‑93 (MQ=255)
                                                             aCCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                            >  1:6522351/1‑93 (MQ=255)
                                                             aCCAAGAAACTCGGCGAGCTGCTCCAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                            <  2:2088486/93‑1 (MQ=255)
                                                              ccATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaa                           >  2:509801/1‑93 (MQ=255)
                                                              ccATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaa                           <  2:28015/93‑1 (MQ=255)
                                                               cATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa                          <  1:6550477/93‑1 (MQ=255)
                                                               cATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa                          >  2:7336515/1‑93 (MQ=255)
                                                                aTGAACCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:4554726/93‑1 (MQ=255)
                                                                aTGAACCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:3853976/93‑1 (MQ=255)
                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:1305497/93‑1 (MQ=255)
                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  1:4116073/93‑1 (MQ=255)
                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:6444692/93‑1 (MQ=255)
                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  1:5757687/93‑1 (MQ=255)
                                                                    aaCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCt                     <  2:5286057/93‑1 (MQ=255)
                                                                     aCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg                    >  2:6860741/1‑93 (MQ=255)
                                                                        cGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGAt                 <  1:6426600/93‑1 (MQ=255)
                                                                        cGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGAt                 <  2:3282109/93‑1 (MQ=255)
                                                                            gAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCaa             >  2:9469646/1‑93 (MQ=255)
                                                                              gctgctGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACa           <  1:10002297/93‑1 (MQ=255)
                                                                              gctgctGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACa           <  1:5452191/93‑1 (MQ=255)
                                                                                tgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGc         >  1:7044788/1‑93 (MQ=255)
                                                                                tgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGc         >  2:1530932/1‑93 (MQ=255)
                                                                                tgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATACAACAgg         >  2:5052303/1‑92 (MQ=255)
                                                                                  ctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGt       <  1:276527/93‑1 (MQ=255)
                                                                                  ctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGt       >  2:404766/1‑93 (MQ=255)
                                                                                   tgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTa      <  1:4273593/93‑1 (MQ=255)
                                                                                    ggAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAt     >  1:3288952/1‑93 (MQ=255)
                                                                                    ggAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAt     >  2:9078264/1‑93 (MQ=255)
                                                                                    ggAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAt     <  1:5257294/93‑1 (MQ=255)
                                                                                     gAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATc    <  2:10003706/93‑1 (MQ=255)
                                                                                      aaGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCg   >  1:5326019/1‑93 (MQ=255)
                                                                                       aGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGa  >  1:367326/1‑93 (MQ=255)
                                                                                       aGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGa  >  1:7685021/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA  >  NC_000913/758315‑758494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAG  >  NC_000913/758312‑758495
                                                                                             |                                                                                          
CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCT                                                                                            <  SRR4302540.3826273/94‑1 (MQ=60)
CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCT                                                                                            >  SRR4302540.5897809/1‑94 (MQ=60)
 GATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTC                                                                                           <  SRR4302540.541557/94‑1 (MQ=60)
  ATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCT                                                                                          <  SRR4302540.4560157/94‑1 (MQ=60)
    CTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCA                                                                                        >  SRR4302540.4112350/1‑94 (MQ=60)
     TGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAA                                                                                       >  SRR4302540.8764441/1‑94 (MQ=60)
     TGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAA                                                                                       <  SRR4302540.6514285/94‑1 (MQ=60)
     TGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAA                                                                                       <  SRR4302540.1489676/94‑1 (MQ=60)
      GACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAG                                                                                      <  SRR4302540.2622703/94‑1 (MQ=60)
      GACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAG                                                                                      <  SRR4302540.1258988/94‑1 (MQ=60)
        CCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCA                                                                                    <  SRR4302540.2178661/94‑1 (MQ=60)
         CGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAA                                                                                   <  SRR4302540.9356686/94‑1 (MQ=60)
         CGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAACATGAAACTCAGCGAGCTGCTGGAAGCTCTCAAGCAA                                                                                   >  SRR4302540.8876989/1‑94 (MQ=60)
          GAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAA                                                                                  <  SRR4302540.258904/94‑1 (MQ=60)
            AGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACC                                                                                <  SRR4302540.5003247/94‑1 (MQ=60)
            AGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACC                                                                                <  SRR4302540.4435368/94‑1 (MQ=60)
            AGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACC                                                                                <  SRR4302540.5054712/94‑1 (MQ=60)
             GCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCT                                                                               >  SRR4302540.4880320/1‑94 (MQ=60)
             GCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCT                                                                               <  SRR4302540.6015296/94‑1 (MQ=60)
             GCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCT                                                                               <  SRR4302540.6387990/94‑1 (MQ=60)
             GCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCT                                                                               <  SRR4302540.4931364/94‑1 (MQ=60)
             GCAGACTTACAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCGGCTGGAAGCTCTCAAGCAAACCT                                                                               <  SRR4302540.5354555/94‑1 (MQ=60)
              CAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTA                                                                              <  SRR4302540.2632966/94‑1 (MQ=60)
               AGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAC                                                                             <  SRR4302540.10047554/94‑1 (MQ=60)
                GACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                            <  SRR4302540.9030388/94‑1 (MQ=60)
                GACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                            <  SRR4302540.4317809/94‑1 (MQ=60)
                GACTTCCAGGAGACCTTCAACGTCGGTTCATCTGCCAGCGTCAAAGAAACCATGAACCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                            <  SRR4302540.4317808/94‑1 (MQ=60)
                 ACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTG                                                                           <  SRR4302540.3223385/94‑1 (MQ=60)
                  CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGC                                                                          <  SRR4302540.9048039/94‑1 (MQ=60)
                  CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGC                                                                          <  SRR4302540.3190795/94‑1 (MQ=60)
                  CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGC                                                                          >  SRR4302540.190183/1‑94 (MQ=60)
                      CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCC                                                                      >  SRR4302540.4032865/1‑94 (MQ=60)
                      CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCC                                                                      >  SRR4302540.4129028/1‑94 (MQ=60)
                      CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCC                                                                      >  SRR4302540.8141015/1‑94 (MQ=60)
                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                     <  SRR4302540.9346923/94‑1 (MQ=60)
                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                     >  SRR4302540.2607040/1‑94 (MQ=60)
                        GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCG                                                                    <  SRR4302540.516125/94‑1 (MQ=60)
                        GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCG                                                                    >  SRR4302540.9633722/1‑94 (MQ=60)
                         GAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGA                                                                   >  SRR4302540.252149/1‑94 (MQ=60)
                          AGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAT                                                                  >  SRR4302540.3214879/1‑94 (MQ=60)
                           GACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATT                                                                 >  SRR4302540.1400749/1‑94 (MQ=60)
                           GACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATT                                                                 >  SRR4302540.6303918/1‑94 (MQ=60)
                           GACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATT                                                                 >  SRR4302540.7792606/1‑94 (MQ=60)
                            ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTG                                                                >  SRR4302540.9381967/1‑94 (MQ=60)
                             CCTTCCACGTCGGTTCATTTGCCAGCCGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTCCTGCGGCCCGATTGG                                                               <  SRR4302540.6910771/94‑1 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               <  SRR4302540.731089/94‑1 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               <  SRR4302540.4538077/94‑1 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               >  SRR4302540.9511403/1‑94 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               <  SRR4302540.710777/94‑1 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               >  SRR4302540.2703759/1‑94 (MQ=60)
                             CCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGG                                                               >  SRR4302540.9331736/1‑94 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGGAAACCTACTGCGGCCCGATTGGT                                                              <  SRR4302540.4433606/94‑1 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGT                                                              <  SRR4302540.9615344/94‑1 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGT                                                              <  SRR4302540.6286502/94‑1 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGT                                                              >  SRR4302540.2133550/1‑94 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGT                                                              >  SRR4302540.4569516/1‑94 (MQ=60)
                              CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGT                                                              >  SRR4302540.4569515/1‑94 (MQ=60)
                               TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTG                                                             >  SRR4302540.7021177/1‑94 (MQ=60)
                                 CAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCC                                                           <  SRR4302540.8855553/94‑1 (MQ=60)
                                 CAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCC                                                           <  SRR4302540.8570459/94‑1 (MQ=60)
                                  AACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCG                                                          <  SRR4302540.63343/94‑1 (MQ=60)
                                   ACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGA                                                         >  SRR4302540.8245817/1‑94 (MQ=60)
                                   ACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGA                                                         >  SRR4302540.8245815/1‑94 (MQ=60)
                                    CGTCGTTTCATTGGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAG                                                        <  SRR4302540.5591202/94‑1 (MQ=60)
                                    CGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAG                                                        <  SRR4302540.5591201/94‑1 (MQ=60)
                                      TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTA                                                      >  SRR4302540.8851806/1‑94 (MQ=60)
                                       CGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAT                                                     <  SRR4302540.4972875/94‑1 (MQ=60)
                                       CGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAT                                                     <  SRR4302540.7512594/94‑1 (MQ=60)
                                       CGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAT                                                     <  SRR4302540.5958682/94‑1 (MQ=60)
                                        GGTTCATTTGTCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATA                                                    >  SRR4302540.8629373/1‑94 (MQ=60)
                                        GGTTCATTTGTCAGCGGCAAAGAAACCATGAAACTCGGCCAGCTGCTGGAGGCTCTCAAGCAAACCTACTGCGGCCCTATTCGTGCCGAcattg                                                    >  SRR4302540.8629667/1‑89 (MQ=60)
                                        GGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATA                                                    >  SRR4302540.4475316/1‑94 (MQ=60)
                                         GTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATAT                                                   <  SRR4302540.9532468/94‑1 (MQ=60)
                                          TTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATG                                                  >  SRR4302540.9223939/1‑94 (MQ=60)
                                          TTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATG                                                  >  SRR4302540.1572499/1‑94 (MQ=60)
                                           TCATTTGCCAGCGGCAAAGAAACCATGAAACTCGTCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGC                                                 <  SRR4302540.1986405/94‑1 (MQ=60)
                                           TCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGC                                                 >  SRR4302540.671442/1‑94 (MQ=60)
                                            CATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCA                                                <  SRR4302540.2506736/94‑1 (MQ=60)
                                             ATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCAC                                               >  SRR4302540.2513428/1‑94 (MQ=60)
                                              TTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACA                                              >  SRR4302540.3696371/1‑94 (MQ=60)
                                               TTGCCAGCGGCAAAGAAACCATGAACCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                             <  SRR4302540.7961885/94‑1 (MQ=60)
                                               TTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAACCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                             <  SRR4302540.9142181/94‑1 (MQ=60)
                                               TTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                             <  SRR4302540.9142180/94‑1 (MQ=60)
                                               TTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                             <  SRR4302540.3446715/94‑1 (MQ=60)
                                                 GCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTA                                           >  SRR4302540.6650819/1‑94 (MQ=60)
                                                     GCGGCAAAGAAACCATGAAACTCGGCTAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAG                                       <  SRR4302540.1679608/94‑1 (MQ=60)
                                                     GCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAG                                       <  SRR4302540.9154227/94‑1 (MQ=60)
                                                      CGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGC                                      <  SRR4302540.3609991/94‑1 (MQ=60)
                                                      CGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGC                                      <  SRR4302540.6464931/94‑1 (MQ=60)
                                                       GGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCA                                     <  SRR4302540.9658653/94‑1 (MQ=60)
                                                       GGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCA                                     <  SRR4302540.9658654/94‑1 (MQ=60)
                                                        GCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGACGAGTATATGCACATTACCAGCAC                                    <  SRR4302540.2307562/94‑1 (MQ=60)
                                                         CAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACC                                   >  SRR4302540.5173121/1‑94 (MQ=60)
                                                         CAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACC                                   >  SRR4302540.2563531/1‑94 (MQ=60)
                                                          AAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCG                                  >  SRR4302540.632330/1‑94 (MQ=60)
                                                          AAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGAAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCG                                  >  SRR4302540.632332/1‑94 (MQ=60)
                                                           AAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGA                                 >  SRR4302540.5170784/1‑94 (MQ=60)
                                                            AGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAA                                <  SRR4302540.7418675/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGTAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.109747/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.701401/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.9963441/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.1197540/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.109748/94‑1 (MQ=60)
                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                               <  SRR4302540.2683363/94‑1 (MQ=60)
                                                               AACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAA                             >  SRR4302540.6594791/1‑94 (MQ=60)
                                                                ACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAA                            <  SRR4302540.4146353/94‑1 (MQ=60)
                                                                  CATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAA                          <  SRR4302540.6623133/94‑1 (MQ=60)
                                                                  CATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAA                          <  SRR4302540.6623134/94‑1 (MQ=60)
                                                                   ATGAAGCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         <  SRR4302540.2495070/94‑1 (MQ=60)
                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         <  SRR4302540.4160508/94‑1 (MQ=60)
                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         <  SRR4302540.5821246/94‑1 (MQ=60)
                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         >  SRR4302540.7186518/1‑94 (MQ=60)
                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         <  SRR4302540.1724083/94‑1 (MQ=60)
                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                         <  SRR4302540.5504213/94‑1 (MQ=60)
                                                                    TGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACG                        >  SRR4302540.2149722/1‑94 (MQ=60)
                                                                    TGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACG                        <  SRR4302540.5784317/94‑1 (MQ=60)
                                                                     GAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGC                       <  SRR4302540.9601985/94‑1 (MQ=60)
                                                                        ACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGG                    <  SRR4302540.1502369/94‑1 (MQ=60)
                                                                           CGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATC                 <  SRR4302540.3373043/94‑1 (MQ=60)
                                                                           CGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATC                 <  SRR4302540.6498132/94‑1 (MQ=60)
                                                                             GCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCA               <  SRR4302540.5590846/94‑1 (MQ=60)
                                                                              CGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAA              >  SRR4302540.4568450/1‑94 (MQ=60)
                                                                               GAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAC             <  SRR4302540.9490355/94‑1 (MQ=60)
                                                                                AGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA            <  SRR4302540.8837043/94‑1 (MQ=60)
                                                                                 GCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAG           <  SRR4302540.10118812/94‑1 (MQ=60)
                                                                                 GCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAG           <  SRR4302540.10118813/94‑1 (MQ=60)
                                                                                 GCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAG           <  SRR4302540.5512195/94‑1 (MQ=60)
                                                                                  CTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGC          >  SRR4302540.7123126/1‑94 (MQ=60)
                                                                                     CTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTA       <  SRR4302540.7866215/94‑1 (MQ=60)
                                                                                     CTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTA       <  SRR4302540.279246/94‑1 (MQ=60)
                                                                                      TGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAT      <  SRR4302540.4320071/94‑1 (MQ=60)
                                                                                      TGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAT      <  SRR4302540.4320070/94‑1 (MQ=60)
                                                                                      TGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAT      >  SRR4302540.3324928/1‑94 (MQ=60)
                                                                                      TGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAT      >  SRR4302540.3324927/1‑94 (MQ=60)
                                                                                      TGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAT      <  SRR4302540.8035257/94‑1 (MQ=60)
                                                                                       GGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATC     <  SRR4302540.534406/94‑1 (MQ=60)
                                                                                       GGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATC     <  SRR4302540.5314657/94‑1 (MQ=60)
                                                                                        GAATCTCTCAAGCAAACCTACTGCGGCCCTATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCG    <  SRR4302540.6950780/94‑1 (MQ=60)
                                                                                        GAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCG    <  SRR4302540.6620034/94‑1 (MQ=60)
                                                                                        GAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCG    >  SRR4302540.5384357/1‑94 (MQ=60)
                                                                                         AAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA   >  SRR4302540.371109/1‑94 (MQ=60)
                                                                                         AAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA   >  SRR4302540.7772165/1‑94 (MQ=60)
                                                                                          AGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAG  <  SRR4302540.3543458/94‑1 (MQ=60)
                                                                                          AGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAG  <  SRR4302540.3253118/94‑1 (MQ=60)
                                                                                             |                                                                                          
CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAG  >  NC_000913/758312‑758495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑