Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	758,405	C→T	A159A (GCC→GCT)	sucA →	2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,405	0	C	T	100.0%	703.6 / NA	190	A159A (GCC→GCT)	sucA	2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (94/96); total (94/96)

Only 100 of 199 total aligned reads displayed.

TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA  >  NC_000913/758315‑758494

tCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGctct                                                                                         <  1:5208380/93‑1 (MQ=255)

tCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGctct                                                                                         >  1:1638422/1‑93 (MQ=255)

 cTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCtctc                                                                                        >  1:4480837/1‑93 (MQ=255)

   gACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCaa                                                                                      >  2:1434479/1‑93 (MQ=255)

   gACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCaa                                                                                      <  1:4002704/93‑1 (MQ=255)

    aCCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAg                                                                                     >  1:5522188/1‑93 (MQ=255)

    aCCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAg                                                                                     <  2:4737548/93‑1 (MQ=255)

      cGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCa                                                                                   >  2:3265506/1‑93 (MQ=255)

       gAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCaa                                                                                  >  1:3749644/1‑93 (MQ=255)

       gAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCaa                                                                                  >  2:4053396/1‑93 (MQ=255)

       gAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGATCTCAAGCaa                                                                                  >  1:4599577/1‑93 (MQ=255)

        aaGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCaaa                                                                                 >  2:1144167/1‑93 (MQ=255)

         aGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAAc                                                                                >  1:2799419/1‑93 (MQ=255)

          gCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAAcc                                                                               <  1:837652/93‑1 (MQ=255)

             gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAc                                                                            >  1:1709337/1‑93 (MQ=255)

             gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAc                                                                            >  2:5788874/1‑93 (MQ=255)

             gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAc                                                                            <  1:864131/93‑1 (MQ=255)

             gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCCGCGAGCTGCTGGAAGCTCCCAAGCAAAGCTat                                                                            >  2:3753218/1‑92 (MQ=255)

              aCTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACt                                                                           >  2:4878122/1‑93 (MQ=255)

              aCTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACt                                                                           <  2:559217/93‑1 (MQ=255)

              aCTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACt                                                                           >  1:504829/1‑93 (MQ=255)

               cTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTg                                                                          >  2:4411205/1‑93 (MQ=255)

                ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGc                                                                         <  2:6058589/93‑1 (MQ=255)

                ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGc                                                                         <  2:2611353/93‑1 (MQ=255)

                ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGc                                                                         >  2:6229126/1‑93 (MQ=255)

                ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGc                                                                         <  2:4445200/93‑1 (MQ=255)

                  ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCgg                                                                       >  1:1489337/1‑93 (MQ=255)

                  ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCgg                                                                       >  1:1191388/1‑93 (MQ=255)

                  ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCgg                                                                       <  1:5826376/93‑1 (MQ=255)

                   cAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGc                                                                      <  2:4257115/93‑1 (MQ=255)

                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     <  1:2915000/93‑1 (MQ=255)

                    aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGcc                                                                     <  1:4944903/93‑1 (MQ=255)

                     ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGccc                                                                    <  2:2587954/93‑1 (MQ=255)

                     ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGccc                                                                    <  2:3412892/93‑1 (MQ=255)

                       agaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGa                                                                  >  1:3000029/1‑93 (MQ=255)

                        gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCGTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAt                                                                 <  2:888122/93‑1 (MQ=255)

                        gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAt                                                                 >  2:4982670/1‑93 (MQ=255)

                        gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAt                                                                 >  2:850503/1‑93 (MQ=255)

                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              <  2:2181461/93‑1 (MQ=255)

                           cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTgg                                                              <  2:5837014/93‑1 (MQ=255)

                             tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                            >  2:4276546/1‑93 (MQ=255)

                             tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                            <  2:3996495/93‑1 (MQ=255)

                                aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCg                                                         >  2:133743/1‑93 (MQ=255)

                                 cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGa                                                        <  1:2229671/93‑1 (MQ=255)

                                 cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGa                                                        <  2:2285331/93‑1 (MQ=255)

                                   tCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGt                                                      <  1:3648063/93‑1 (MQ=255)

                                    cGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGta                                                     <  2:3451466/93‑1 (MQ=255)

                                     ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtat                                                    >  2:330025/1‑93 (MQ=255)

                                      gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                                   <  2:1805076/93‑1 (MQ=255)

                                      gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                                   <  2:2938516/93‑1 (MQ=255)

                                      gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                                   >  2:402325/1‑93 (MQ=255)

                                       ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                                  <  2:411069/93‑1 (MQ=255)

                                        tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATg                                                 >  1:1159802/1‑93 (MQ=255)

                                          aTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGca                                               >  1:5633575/1‑93 (MQ=255)

                                            ttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                             <  2:1096115/93‑1 (MQ=255)

                                                cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAc                                         >  1:5453030/1‑93 (MQ=255)

                                                cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAc                                         <  2:2253749/93‑1 (MQ=255)

                                                 aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                                        >  1:1991678/1‑93 (MQ=255)

                                                 aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                                        >  1:732207/1‑93 (MQ=255)

                                                   cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAg                                      <  2:720109/93‑1 (MQ=255)

                                                       aaacaaacCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                                  <  1:6078636/93‑1 (MQ=255)

                                                         aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                                <  1:5681203/93‑1 (MQ=255)

                                                          gAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               >  1:3473621/1‑93 (MQ=255)

                                                          gAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               >  1:306965/1‑93 (MQ=255)

                                                          gAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                               >  2:5386767/1‑93 (MQ=255)

                                                             aCCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                            >  1:3921970/1‑93 (MQ=255)

                                                             aCCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                            >  2:3689545/1‑93 (MQ=255)

                                                              ccATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaa                           >  1:4136800/1‑93 (MQ=255)

                                                                ctGAAACGCGGCGAGCTGCTGGAAGCTCTCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGaaaaa                         <  1:4164981/92‑1 (MQ=255)

                                                                aTGAAGCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:5930739/93‑1 (MQ=255)

                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:5224784/93‑1 (MQ=255)

                                                                aTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                         <  2:1955483/93‑1 (MQ=255)

                                                                 tGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc                        <  1:1756369/93‑1 (MQ=255)

                                                                  gAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACg                       >  2:6196395/1‑93 (MQ=255)

                                                                   aaaCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGc                      <  2:1684438/93‑1 (MQ=255)

                                                                     aCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg                    <  2:5325210/93‑1 (MQ=255)

                                                                     aCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg                    <  2:2127715/93‑1 (MQ=255)

                                                                     aCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg                    <  2:4284843/93‑1 (MQ=255)

                                                                      cTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg                   <  1:6221878/93‑1 (MQ=255)

                                                                       tCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGa                  >  1:1072753/1‑93 (MQ=255)

                                                                         ggCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc                <  2:928548/93‑1 (MQ=255)

                                                                         ggCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc                >  1:4304621/1‑93 (MQ=255)

                                                                            gAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCaa             >  1:2120025/1‑93 (MQ=255)

                                                                             aGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAc            <  1:3208162/93‑1 (MQ=255)

                                                                             aGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAc            <  2:5425387/93‑1 (MQ=255)

                                                                             aGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAc            >  1:5925462/1‑93 (MQ=255)

                                                                             aGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAc            <  2:3586138/93‑1 (MQ=255)

                                                                              gctgctGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACa           <  1:1042476/93‑1 (MQ=255)

                                                                              gctgctGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACa           <  2:1931520/93‑1 (MQ=255)

                                                                               ctgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAg          <  1:3519631/93‑1 (MQ=255)

                                                                                tgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGc         >  1:1449575/1‑93 (MQ=255)

                                                                                tgctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGc         >  1:1307751/1‑93 (MQ=255)

                                                                                  ctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGt       >  2:91873/1‑93 (MQ=255)

                                                                                  ctgGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGt       >  2:921077/1‑93 (MQ=255)

                                                                                     gAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATc    <  2:2438237/93‑1 (MQ=255)

                                                                                     gAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATc    <  1:2467237/93‑1 (MQ=255)

                                                                                     gAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATc    >  2:2788450/1‑93 (MQ=255)

                                                                                      aaGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCg   <  1:4827396/93‑1 (MQ=255)

                                                                                       aGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGa  >  2:265600/1‑93 (MQ=255)

                                                                                       aGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGa  <  2:3492382/93‑1 (MQ=255)

TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA  >  NC_000913/758315‑758494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCT  >  NC_000913/758312‑758498

CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCT                                                                                               <  SRR4302533.1845473/94‑1 (MQ=60)

 GATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTC                                                                                              >  SRR4302533.2860588/1‑94 (MQ=60)

  ATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCT                                                                                             >  SRR4302533.1648341/1‑94 (MQ=60)

  ATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCT                                                                                             <  SRR4302533.180350/94‑1 (MQ=60)

   TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTC                                                                                            <  SRR4302533.5239756/94‑1 (MQ=60)

   TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTC                                                                                            >  SRR4302533.5453095/1‑94 (MQ=60)

   TCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTC                                                                                            >  SRR4302533.4507945/1‑94 (MQ=60)

      GACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAG                                                                                         >  SRR4302533.5555532/1‑94 (MQ=60)

      GACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAG                                                                                         <  SRR4302533.4027077/94‑1 (MQ=60)

         CGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAA                                                                                      >  SRR4302533.3772630/1‑94 (MQ=60)

         CGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAA                                                                                      >  SRR4302533.2508745/1‑94 (MQ=60)

         CGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGATCTCAAGCAA                                                                                      >  SRR4302533.4627442/1‑94 (MQ=60)

           AAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAAC                                                                                    >  SRR4302533.2816638/1‑94 (MQ=60)

             GCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCT                                                                                  <  SRR4302533.842560/94‑1 (MQ=60)

               AGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTAC                                                                                >  SRR4302533.1719694/1‑94 (MQ=60)

                GACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                               >  SRR4302533.507824/1‑94 (MQ=60)

                GACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                               <  SRR4302533.869193/94‑1 (MQ=60)

                GACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACT                                                                               <  SRR4302533.6321869/94‑1 (MQ=60)

                 ACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTG                                                                              <  SRR4302533.4992050/94‑1 (MQ=60)

                  CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGC                                                                             <  SRR4302533.5934796/94‑1 (MQ=60)

                   TTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCG                                                                            >  SRR4302533.3139915/1‑94 (MQ=60)

                    TCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGG                                                                           >  SRR4302533.1498321/1‑94 (MQ=60)

                    TCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGG                                                                           >  SRR4302533.1198546/1‑94 (MQ=60)

                     CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGC                                                                          <  SRR4302533.5861582/94‑1 (MQ=60)

                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                        <  SRR4302533.2932880/94‑1 (MQ=60)

                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                        <  SRR4302533.4974831/94‑1 (MQ=60)

                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                        >  SRR4302533.2204821/1‑94 (MQ=60)

                       AGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCC                                                                        >  SRR4302533.1302695/1‑94 (MQ=60)

                        GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCG                                                                       >  SRR4302533.4163809/1‑94 (MQ=60)

                        GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCG                                                                       <  SRR4302533.1103943/94‑1 (MQ=60)

                         GAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGA                                                                      >  SRR4302533.3018376/1‑94 (MQ=60)

                          AGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAT                                                                     >  SRR4302533.5667695/1‑94 (MQ=60)

                          AGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGAT                                                                     <  SRR4302533.5592810/94‑1 (MQ=60)

                            ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTG                                                                   >  SRR4302533.4198028/1‑94 (MQ=60)

                               TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTG                                                                <  SRR4302533.2125765/94‑1 (MQ=60)

                                 CAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCC                                                              <  SRR4302533.3889683/94‑1 (MQ=60)

                                 CAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCC                                                              >  SRR4302533.346601/1‑94 (MQ=60)

                                    CGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAG                                                           >  SRR4302533.3729290/1‑94 (MQ=60)

                                    CGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAG                                                           >  SRR4302533.824113/1‑94 (MQ=60)

                                    CGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAG                                                           <  SRR4302533.2243433/94‑1 (MQ=60)

                                     GTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGT                                                          <  SRR4302533.4774781/94‑1 (MQ=60)

                                      TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTA                                                         <  SRR4302533.3670493/94‑1 (MQ=60)

                                       CGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAT                                                        >  SRR4302533.4591517/1‑94 (MQ=60)

                                        GGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATA                                                       >  SRR4302533.848728/1‑94 (MQ=60)

                                        GGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATA                                                       >  SRR4302533.193831/1‑94 (MQ=60)

                                         GTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATAT                                                      >  SRR4302533.3955017/1‑94 (MQ=60)

                                          TTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATG                                                     >  SRR4302533.1166761/1‑94 (MQ=60)

                                          TTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATG                                                     <  SRR4302533.6227648/94‑1 (MQ=60)

                                            CATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCA                                                   >  SRR4302533.5667643/1‑94 (MQ=60)

                                               TTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                                <  SRR4302533.234210/94‑1 (MQ=60)

                                               TTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAT                                                <  SRR4302533.1672899/94‑1 (MQ=60)

                                                 GCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTA                                              <  SRR4302533.1347441/94‑1 (MQ=60)

                                                  CCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAC                                             >  SRR4302533.5485875/1‑94 (MQ=60)

                                                   CAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACC                                            >  SRR4302533.2003885/1‑94 (MQ=60)

                                                   CAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACC                                            >  SRR4302533.736516/1‑94 (MQ=60)

                                                    AGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCA                                           <  SRR4302533.1733855/94‑1 (MQ=60)

                                                    AGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCA                                           >  SRR4302533.2986852/1‑94 (MQ=60)

                                                     GCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAG                                          <  SRR4302533.5998685/94‑1 (MQ=60)

                                                      CGGCAAAGAAACCTTGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGC                                         <  SRR4302533.3624990/94‑1 (MQ=60)

                                                          AAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCG                                     <  SRR4302533.1196851/94‑1 (MQ=60)

                                                          AAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCG                                     <  SRR4302533.3524553/94‑1 (MQ=60)

                                                          AAACAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCG                                     <  SRR4302533.6115399/94‑1 (MQ=60)

                                                           AAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGA                                    <  SRR4302533.4127514/94‑1 (MQ=60)

                                                            AGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAA                                   <  SRR4302533.5715518/94‑1 (MQ=60)

                                                            AGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAA                                   >  SRR4302533.3495056/1‑94 (MQ=60)

                                                            AGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAA                                   >  SRR4302533.1491911/1‑94 (MQ=60)

                                                            AGAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAA                                   >  SRR4302533.308843/1‑94 (MQ=60)

                                                             GAAACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAG                                  <  SRR4302533.5118576/94‑1 (MQ=60)

                                                               AACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAA                                >  SRR4302533.3945920/1‑94 (MQ=60)

                                                                ACCATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAA                               >  SRR4302533.4161904/1‑94 (MQ=60)

                                                                   CTGAAACGCGGCGAGCTGCTGGAAGCTCTCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGAAAAAC                            <  SRR4302533.4190216/94‑1 (MQ=60)

                                                                   ATGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAC                            <  SRR4302533.3045704/94‑1 (MQ=60)

                                                                    TGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACG                           <  SRR4302533.1552200/94‑1 (MQ=60)

                                                                    TGAAACTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACG                           <  SRR4302533.1767068/94‑1 (MQ=60)

                                                                       AGCTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTG                        <  SRR4302533.445245/94‑1 (MQ=60)

                                                                         CTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGA                      >  SRR4302533.1079076/1‑94 (MQ=60)

                                                                         CTCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGA                      <  SRR4302533.6259170/94‑1 (MQ=60)

                                                                          TCGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGAT                     <  SRR4302533.2874086/94‑1 (MQ=60)

                                                                           CGGCGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATC                    >  SRR4302533.4330521/1‑94 (MQ=60)

                                                                              CGAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAA                 >  SRR4302533.2133099/1‑94 (MQ=60)

                                                                               GAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAC                <  SRR4302533.5490442/94‑1 (MQ=60)

                                                                               GAGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAC                >  SRR4302533.5961284/1‑94 (MQ=60)

                                                                                AGCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA               <  SRR4302533.3227919/94‑1 (MQ=60)

                                                                                 GCTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAG              <  SRR4302533.1048605/94‑1 (MQ=60)

                                                                                  CTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGC             >  SRR4302533.1315603/1‑94 (MQ=60)

                                                                                  CTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGC             >  SRR4302533.1458323/1‑94 (MQ=60)

                                                                                  CTGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGC             <  SRR4302533.3541329/94‑1 (MQ=60)

                                                                                   TGCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCG            >  SRR4302533.57760/1‑94 (MQ=60)

                                                                                    GCTGGAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGT           >  SRR4302533.4925657/1‑94 (MQ=60)

                                                                                        GAAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCG       <  SRR4302533.2482456/94‑1 (MQ=60)

                                                                                         AAGCTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGA      <  SRR4302533.4856650/94‑1 (MQ=60)

                                                                                           GCTCTCAAGCAAACCCACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGT    >  SRR4302533.5844629/1‑94 (MQ=60)

                                                                                            CTCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTC   <  SRR4302533.5870120/94‑1 (MQ=60)

                                                                                             TCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCT  >  SRR4302533.5731643/1‑94 (MQ=60)

                                                                                             TCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCT  <  SRR4302533.2305066/94‑1 (MQ=60)

                                                                                             TCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCT  >  SRR4302533.524908/1‑94 (MQ=60)

CGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCT  >  NC_000913/758312‑758498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`