Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 807,510 A→G L324P (CTG→CCG)  bioA ← 7,8‑diaminopelargonic acid synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913807,5100AG92.1% 526.9 / 27.6 204L324P (CTG→CCG) bioA7,8‑diaminopelargonic acid synthetase
Reads supporting (aligned to +/- strand):  ref base A (5/11);  new base G (99/88);  total (105/99)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 209 total aligned reads displayed.

CGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTC  >  NC_000913/807421‑807601
                                                                                         |                                                                                           
cGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGc                                                                                          <  2:3263374/93‑1 (MQ=255)
cGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGc                                                                                          >  1:4185013/1‑93 (MQ=255)
  gCATCACGGGCGGGGTCAAGTGGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTg                                                                                        <  2:8838460/93‑1 (MQ=255)
  gCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTg                                                                                        <  2:4125508/93‑1 (MQ=255)
  gCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTg                                                                                        >  1:770691/1‑93 (MQ=255)
  gCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTg                                                                                        >  2:8211277/1‑93 (MQ=255)
   cATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTgg                                                                                       <  1:7456044/93‑1 (MQ=255)
    aTCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGc                                                                                      >  2:4609869/1‑93 (MQ=255)
     tCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCg                                                                                     >  1:3642295/1‑93 (MQ=255)
     tCACGGGCGGGGGCAAGTTGCTCCCGCCGCTGTACTTCAATATCCGCCACCTGTTGCTCCCAGTCGCCAGATTCGAGGCTCGCCTGGCTcgcg                                                                                     >  1:3486042/1‑93 (MQ=255)
       ccgggcgggGGCAAGGTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGtt                                                                                   <  1:1444951/92‑1 (MQ=255)
       aCGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCgac                                                                                   <  2:138218/93‑3 (MQ=255)
          ggcgggGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTtgc                                                                                <  1:234856/93‑1 (MQ=255)
           gcgggGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTtgct                                                                               >  1:8026874/1‑93 (MQ=255)
           gcgggGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTtgct                                                                               <  2:6856937/93‑1 (MQ=255)
               gggCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGCTTGCTGccc                                                                           >  1:837814/1‑92 (MQ=255)
                 gCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCcgcg                                                                         >  1:1119676/1‑93 (MQ=255)
                   aaGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCa                                                                       >  1:3318153/1‑93 (MQ=255)
                   aaGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCa                                                                       >  1:7579458/1‑93 (MQ=255)
                   aaGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCa                                                                       <  2:6864714/93‑1 (MQ=255)
                    aGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAg                                                                      >  1:2673316/1‑93 (MQ=255)
                    aGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCCGGCTGGCGTTTGCTGCCGCGCAg                                                                      >  2:2559624/1‑93 (MQ=255)
                      ttGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGc                                                                    >  2:904990/1‑93 (MQ=255)
                       tGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGcc                                                                   >  2:3795183/1‑93 (MQ=255)
                       tGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGGTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGcc                                                                   <  1:3876300/93‑1 (MQ=255)
                        gCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCa                                                                  <  1:9051119/93‑1 (MQ=255)
                         cTCGCGCGGCTGTACTTCAATATCCGCCGCCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAg                                                                 <  2:7612868/93‑1 (MQ=255)
                         cTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAg                                                                 <  1:6151170/93‑1 (MQ=255)
                          tCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGc                                                                >  1:2783412/1‑93 (MQ=255)
                          tCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGc                                                                <  1:72066/93‑1 (MQ=255)
                            gcgcAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCgg                                                              <  2:9334858/93‑1 (MQ=255)
                             cgcAGCTGTACTTAAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCTTTTGCTGCCGCGCAGGCCAGCGGa                                                             <  1:2390850/93‑1 (MQ=255)
                              gcAGCTGTACTTCAATATCCGCTGGCTGTTCCTGCCAGTCGCCCGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCggtt                                                            <  2:4822440/93‑3 (MQ=255)
                              gcAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAt                                                            <  1:2179270/93‑1 (MQ=255)
                              gcAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAt                                                            <  1:4690341/93‑1 (MQ=255)
                              gcAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAt                                                            <  2:1113194/93‑1 (MQ=255)
                              ccAGCTGTACTTCAATATCCGCCCCCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAt                                                            <  2:5529349/92‑1 (MQ=255)
                               cAGCTGTGCTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAtt                                                           <  1:9134831/93‑1 (MQ=255)
                               cAGCTGTACTTCAATATCGGCCACCTGTTGCTGCCAGTCGCCAGATGCGAGAATCGCCAGGCTGGCGCTTGCTGGCGCGCGGCGCAGGGGAtg                                                           >  1:6952033/1‑92 (MQ=255)
                                aGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTg                                                          >  1:5055644/1‑93 (MQ=255)
                                   tGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGccc                                                       >  2:3949057/1‑93 (MQ=255)
                                   tGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGccc                                                       >  1:6559749/1‑93 (MQ=255)
                                    gTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCa                                                      >  2:7288092/1‑93 (MQ=255)
                                    gTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCa                                                      >  1:3074333/1‑93 (MQ=255)
                                    gTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCa                                                      <  1:4651448/93‑1 (MQ=255)
                                    gTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCGGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCa                                                      <  2:5604509/93‑1 (MQ=255)
                                     tACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCAt                                                     <  1:8224775/93‑1 (MQ=255)
                                      aCTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATa                                                    >  2:7896618/1‑93 (MQ=255)
                                      aCTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATa                                                    >  2:8341972/1‑93 (MQ=255)
                                       cTTTAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaa                                                   <  2:5823598/93‑1 (MQ=255)
                                       cTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaa                                                   >  2:2826855/1‑93 (MQ=255)
                                        ttCAATATCCGCCGCCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaaa                                                  <  1:9277826/93‑1 (MQ=255)
                                        ttCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaaa                                                  >  2:908327/1‑93 (MQ=255)
                                        ttCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGCTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaaa                                                  >  2:267668/1‑93 (MQ=255)
                                         tCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATaaaa                                                 <  2:6967146/93‑1 (MQ=255)
                                          cAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAg                                                >  1:526861/1‑93 (MQ=255)
                                          cAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAg                                                <  2:1868283/93‑1 (MQ=255)
                                          cAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAg                                                <  2:5414389/93‑1 (MQ=255)
                                          cAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCA‑GCCAGCGGATTGCCCCTtaaaat                                               >  1:6477275/1‑91 (MQ=255)
                                             tatCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTg                                             >  2:9318500/1‑93 (MQ=255)
                                              atCCGCCACCTGTTGCTGGCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGGCCATAAAAGTTgg                                            >  2:6412012/1‑93 (MQ=255)
                                               tCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGc                                           >  2:1569013/1‑93 (MQ=255)
                                               tCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGc                                           <  1:702886/93‑1 (MQ=255)
                                               tCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGc                                           >  2:5109939/1‑93 (MQ=255)
                                                   ccACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCAt                                       <  2:631181/93‑1 (MQ=255)
                                                   ccACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCAt                                       <  2:7789649/93‑1 (MQ=255)
                                                   ccACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCAt                                       <  1:6295137/93‑1 (MQ=255)
                                                    cACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGGTTGCTGCCGCGCAGGCCAGCGGGTTGCCCATAAAAGTTGGCCCaag                                      >  1:2100702/1‑91 (MQ=255)
                                                      ccTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCCTGCa                                    >  1:2043521/1‑93 (MQ=255)
                                                      ccTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCCTGCa                                    >  2:3724800/1‑93 (MQ=255)
                                                      ccTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCa                                    >  2:6137475/1‑93 (MQ=255)
                                                        tGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATa                                  >  1:8767951/1‑93 (MQ=255)
                                                            gctgcCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGc                              <  1:4468073/93‑1 (MQ=255)
                                                              tgcCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCaa                            <  2:494000/93‑1 (MQ=255)
                                                              tgcCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCaa                            <  2:7478312/93‑1 (MQ=255)
                                                               gcCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAAc                           <  1:9086000/93‑1 (MQ=255)
                                                               gcCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAAc                           <  1:1892493/93‑1 (MQ=255)
                                                                ccAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAAcc                          >  1:6130884/1‑93 (MQ=255)
                                                                 cAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCg                         >  2:5302468/1‑93 (MQ=255)
                                                                 cAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCg                         <  1:9638205/93‑1 (MQ=255)
                                                                    tCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCt                      >  1:6762211/1‑93 (MQ=255)
                                                                     cGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCtt                     <  2:8683733/93‑1 (MQ=255)
                                                                      gCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTc                    >  2:1685371/1‑93 (MQ=255)
                                                                      gCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTAGCCCATGCATAAAGCAACCCGCTTc                    >  2:4367766/1‑93 (MQ=255)
                                                                       ccAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCa                   >  1:1631806/1‑93 (MQ=255)
                                                                        cAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCAc                  <  2:7040014/93‑1 (MQ=255)
                                                                          gATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCg                >  2:7807636/1‑93 (MQ=255)
                                                                           aTTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGt               >  2:7164075/1‑93 (MQ=255)
                                                                           aTTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGt               >  2:4017609/1‑93 (MQ=255)
                                                                            ttCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGtt              <  2:330820/93‑1 (MQ=255)
                                                                             tCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTa             >  1:9906074/1‑93 (MQ=255)
                                                                             tCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTa             >  1:6665390/1‑93 (MQ=255)
                                                                             tCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAACAACCGGCTTCACCGTTa             >  2:7588287/1‑93 (MQ=255)
                                                                              cGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTAc            <  2:2406325/93‑1 (MQ=255)
                                                                               gagaATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAAACGGCTTCACCGTTACt           >  1:7274434/1‑93 (MQ=255)
                                                                                agaATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTg          <  2:6625917/93‑1 (MQ=255)
                                                                                   aTCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATg       <  2:4265842/93‑1 (MQ=255)
                                                                                   aTCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATg       <  2:7975163/93‑1 (MQ=255)
                                                                                    tCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATgg      >  1:4561200/1‑93 (MQ=255)
                                                                                        cAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTc  <  2:3133438/93‑1 (MQ=255)
                                                                                         |                                                                                           
CGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTC  >  NC_000913/807421‑807601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  NC_000913/807417‑807603
                                                                                             |                                                                                             
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCCGTCGCCAGATTCGAGTATCGCCG                                                                                               >  SRR4302540.1484390/1‑94 (MQ=60)
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCG                                                                                               >  SRR4302540.8599275/1‑94 (MQ=60)
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCG                                                                                               >  SRR4302540.2953868/1‑94 (MQ=60)
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCG                                                                                               <  SRR4302540.9746698/94‑1 (MQ=60)
   TCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGC                                                                                            >  SRR4302540.4230490/1‑94 (MQ=60)
    CGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTGGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCT                                                                                           <  SRR4302540.4302784/94‑1 (MQ=60)
     GGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCCGATTCGAGAATCGCCGCGCTG                                                                                          >  SRR4302540.2079511/1‑94 (MQ=60)
     GGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTG                                                                                          >  SRR4302540.778892/1‑94 (MQ=60)
       CATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGC                                                                                        <  SRR4302540.7539173/94‑1 (MQ=60)
        CTCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCG                                                                                       <  SRR4302540.2359566/94‑1 (MQ=60)
        ATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCG                                                                                       >  SRR4302540.3682186/1‑94 (MQ=60)
        ATCACGGGCGGGGGCAAGTTGCTCCCGCCGCTGTACTTCAATATCCGCCACCTGTTGCTCCCAGTCGCCAGATTCGAGGCTCGCCTGGCTCGCG                                                                                       >  SRR4302540.3524007/1‑94 (MQ=60)
        ATCACGAGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCG                                                                                       <  SRR4302540.3761551/94‑1 (MQ=60)
           CCGGGCGGGGGCAAGGTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTT                                                                                    <  SRR4302540.1460659/94‑1 (MQ=60)
             GGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGC                                                                                  >  SRR4302540.3528847/1‑94 (MQ=60)
              GGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCT                                                                                 >  SRR4302540.7564303/1‑94 (MQ=60)
              GGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCT                                                                                 <  SRR4302540.237131/94‑1 (MQ=60)
              GGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCT                                                                                 >  SRR4302540.8120218/1‑94 (MQ=60)
                  GGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGCTTGCTGCCC                                                                             >  SRR4302540.846707/1‑94 (MQ=60)
                    GGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCG                                                                           >  SRR4302540.1131290/1‑94 (MQ=60)
                      CAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCA                                                                         >  SRR4302540.7664517/1‑94 (MQ=60)
                      CAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCA                                                                         >  SRR4302540.3354542/1‑94 (MQ=60)
                      CAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCA                                                                         >  SRR4302540.9627070/1‑94 (MQ=60)
                       AAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAG                                                                        <  SRR4302540.916227/94‑1 (MQ=60)
                       AAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAG                                                                        >  SRR4302540.2702262/1‑94 (MQ=60)
                        AGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGG                                                                       <  SRR4302540.2988778/94‑1 (MQ=60)
                         GTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGC                                                                      >  SRR4302540.5657501/1‑94 (MQ=60)
                          TTGCTCGCCCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCC                                                                     <  SRR4302540.8805093/94‑1 (MQ=60)
                           TGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCA                                                                    <  SRR4302540.486929/94‑1 (MQ=60)
                           TGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGGTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCA                                                                    <  SRR4302540.3918432/94‑1 (MQ=60)
                            GCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAG                                                                   <  SRR4302540.9159773/94‑1 (MQ=60)
                             CTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGC                                                                  <  SRR4302540.10003998/94‑1 (MQ=60)
                             CTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGC                                                                  >  SRR4302540.2813477/1‑94 (MQ=60)
                             CTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGC                                                                  <  SRR4302540.6219680/94‑1 (MQ=60)
                              TCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCG                                                                 <  SRR4302540.72805/94‑1 (MQ=60)
                              TCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCG                                                                 <  SRR4302540.3257799/94‑1 (MQ=60)
                              TCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCG                                                                 <  SRR4302540.7101134/94‑1 (MQ=60)
                                 CGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGAT                                                              >  SRR4302540.9295192/1‑94 (MQ=60)
                                 CGCAGCTGTACTTAAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCTTTTGCTGCCGCGCAGGCCAGCGGAT                                                              <  SRR4302540.2416617/94‑1 (MQ=60)
                                  GCAGCTGTACTTCAATATCGGCCACCTGTTGCTGCCAGTCGCCAGATGCGAGAATCGCCAGGCTGGCGCTTGCTGGCGCGCggcgcaggggatg                                                             >  SRR4302540.7029386/1‑81 (MQ=60)
                                  GCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATT                                                             <  SRR4302540.6630098/94‑1 (MQ=60)
                                  GCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATT                                                             <  SRR4302540.2202774/94‑1 (MQ=60)
                                  GCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATT                                                             <  SRR4302540.4740495/94‑1 (MQ=60)
                                   CAGCTGTGCTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTG                                                            <  SRR4302540.9244609/94‑1 (MQ=60)
                                   CAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTG                                                            >  SRR4302540.5110302/1‑94 (MQ=60)
                                    AGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGC                                                           >  SRR4302540.7371363/1‑94 (MQ=60)
                                      CTGTACTTCAATATCCGCCACCTGTTGCTTCCAGTCGCCAGATTCGAGAATCGCCCGCCTAGCGTTTGCTGACTCGCAGGACAGCGAATTGCAC                                                         >  SRR4302540.6632474/1‑94 (MQ=60)
                                      CTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCC                                                         >  SRR4302540.6632475/1‑94 (MQ=60)
                                       TGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCA                                                        >  SRR4302540.3108058/1‑94 (MQ=60)
                                       TGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGCTTGCTGCCGCGCAGGCCAGCGGATTGCCCA                                                        >  SRR4302540.8427943/1‑94 (MQ=60)
                                        GTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCAT                                                       <  SRR4302540.4701225/94‑1 (MQ=60)
                                         TACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATA                                                      <  SRR4302540.8320387/94‑1 (MQ=60)
                                           CTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAA                                                    >  SRR4302540.2391685/1‑94 (MQ=60)
                                            TTCAATATCCGCCGCCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAA                                                   <  SRR4302540.9388736/94‑1 (MQ=60)
                                            TTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAA                                                   <  SRR4302540.1955167/94‑1 (MQ=60)
                                             TCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAG                                                  >  SRR4302540.532557/1‑94 (MQ=60)
                                             TCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCA‑GCCAGCGGATTGCCCcttaaaat                                                 >  SRR4302540.6549339/1‑86 (MQ=60)
                                              CAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGT                                                 >  SRR4302540.7472473/1‑94 (MQ=60)
                                              CAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGT                                                 >  SRR4302540.6932871/1‑94 (MQ=60)
                                              CAATAGCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGT                                                 <  SRR4302540.1513414/94‑1 (MQ=60)
                                                 TATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGG                                              <  SRR4302540.2147810/94‑1 (MQ=60)
                                                 TATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGG                                              >  SRR4302540.7690318/1‑94 (MQ=60)
                                                  ATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGC                                             <  SRR4302540.8306718/94‑1 (MQ=60)
                                                   TCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCC                                            <  SRR4302540.710430/94‑1 (MQ=60)
                                                     CGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCA                                          >  SRR4302540.6176296/1‑94 (MQ=60)
                                                      GCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCAT                                         <  SRR4302540.6261829/94‑1 (MQ=60)
                                                       CCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATG                                        <  SRR4302540.6365227/94‑1 (MQ=60)
                                                       CCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGGTTGCTGCCGCGCAGGCCAGCGGGTTGCCCATAAAAGTTGGCCCAAG                                        >  SRR4302540.2123254/1‑94 (MQ=60)
                                                         ACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCCTGCA                                      >  SRR4302540.2065534/1‑94 (MQ=60)
                                                           CTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCGCATGCATA                                    >  SRR4302540.2577128/1‑94 (MQ=60)
                                                           CTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATA                                    >  SRR4302540.7251097/1‑94 (MQ=60)
                                                           CTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATA                                    >  SRR4302540.8869579/1‑94 (MQ=60)
                                                           CTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATA                                    <  SRR4302540.7330443/94‑1 (MQ=60)
                                                            TGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAA                                   >  SRR4302540.3397391/1‑94 (MQ=60)
                                                            TGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAA                                   >  SRR4302540.8040452/1‑94 (MQ=60)
                                                             GTTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAA                                  >  SRR4302540.3326834/1‑94 (MQ=60)
                                                              TTGCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAG                                 <  SRR4302540.4190035/94‑1 (MQ=60)
                                                                GCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCA                               >  SRR4302540.620646/1‑94 (MQ=60)
                                                                GCTGCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCA                               <  SRR4302540.4516515/94‑1 (MQ=60)
                                                                   GCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACC                            <  SRR4302540.1913210/94‑1 (MQ=60)
                                                                   GCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACC                            >  SRR4302540.6199170/1‑94 (MQ=60)
                                                                   GCCAGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACC                            <  SRR4302540.9195157/94‑1 (MQ=60)
                                                                     CAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGG                          <  SRR4302540.9751747/94‑1 (MQ=60)
                                                                      AGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGC                         >  SRR4302540.9198711/1‑94 (MQ=60)
                                                                      AGTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGC                         <  SRR4302540.4473985/94‑1 (MQ=60)
                                                                       GTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCT                        >  SRR4302540.6836944/1‑94 (MQ=60)
                                                                       GTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCT                        <  SRR4302540.5147459/94‑1 (MQ=60)
                                                                       GTCGCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACAGGCT                        >  SRR4302540.6836943/1‑94 (MQ=60)
                                                                       GTCGCCAGATTCGAGAATCGCCGGGCTGGCGGGTGCCGCCGCGCAGGCCAGCGGAGAGCCCACAAAAGTTGGCCCATGCATAAAGCAACCGGCG                        >  SRR4302540.9024952/1‑94 (MQ=60)
                                                                          GCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCA                     >  SRR4302540.1649663/1‑94 (MQ=60)
                                                                          GCCAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCA                     >  SRR4302540.1649664/1‑94 (MQ=60)
                                                                           CCAGATTCGAGAATCGCCGGGCTGGCGTTTGCAGCCGGGCAGGCCAGCGGATCGCCCCTAAACGTTGGCCCATGCATAAAGCAACCGGCTTCAC                    >  SRR4302540.3639260/1‑94 (MQ=60)
                                                                            CAGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACC                   >  SRR4302540.972062/1‑94 (MQ=60)
                                                                             AGATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCG                  >  SRR4302540.6491234/1‑94 (MQ=60)
                                                                              GATTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTAACCGT                 >  SRR4302540.127089/1‑94 (MQ=60)
                                                                                TTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTA               >  SRR4302540.6739062/1‑94 (MQ=60)
                                                                                TTCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTA               >  SRR4302540.10021798/1‑94 (MQ=60)
                                                                                 TCGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTAC              <  SRR4302540.6717019/94‑1 (MQ=60)
                                                                                  CGAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAAACGGCTTCACCGTTACT             >  SRR4302540.7355606/1‑94 (MQ=60)
                                                                                   GAGAATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTG            <  SRR4302540.4726819/94‑1 (MQ=60)
                                                                                       ATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGG        >  SRR4302540.4610481/1‑94 (MQ=60)
                                                                                       ATCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGG        >  SRR4302540.4610482/1‑94 (MQ=60)
                                                                                        TCGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGT       <  SRR4302540.2770379/94‑1 (MQ=60)
                                                                                         CGCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTT      >  SRR4302540.4690892/1‑94 (MQ=60)
                                                                                          GCCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTT     >  SRR4302540.9407287/1‑94 (MQ=60)
                                                                                           CCGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTC    >  SRR4302540.694641/1‑94 (MQ=60)
                                                                                            CGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCT   <  SRR4302540.9325279/94‑1 (MQ=60)
                                                                                            CGGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCT   <  SRR4302540.501607/94‑1 (MQ=60)
                                                                                             GGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  SRR4302540.2300633/1‑94 (MQ=60)
                                                                                             GGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  SRR4302540.1635882/1‑94 (MQ=60)
                                                                                             GGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  SRR4302540.3356578/1‑94 (MQ=60)
                                                                                             GGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  SRR4302540.3721486/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTG  >  NC_000913/807417‑807603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑