Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	810,837	T→C	A31A (GCT→GCC)	bioC →	BioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	810,837	0	T	C	99.6%	724.3 / NA	223	A31A (GCT→GCC)	bioC	BioC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (125/97); minor base A (0/1); total (125/98)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCG  >  NC_000913/810747‑810926

 gCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGAc                                                                                        <  1:9943708/93‑1 (MQ=255)

 gCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGAc                                                                                        <  2:3850904/93‑1 (MQ=255)

 gCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGAc                                                                                        <  1:537471/93‑1 (MQ=255)

   aaCGGTTAATAACCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGc                                                                                      <  2:4444929/93‑1 (MQ=255)

    aCGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGTCAGAGTGCCGACGcc                                                                                     <  2:144891/93‑1 (MQ=255)

    aCGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGcc                                                                                     >  1:4599975/1‑93 (MQ=255)

       gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTa                                                                                  >  2:8299584/1‑93 (MQ=255)

        ttAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAc                                                                                 >  2:746849/1‑93 (MQ=255)

        ttAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAc                                                                                 >  1:2332880/1‑93 (MQ=255)

        ttAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAc                                                                                 <  2:6229934/93‑1 (MQ=255)

          aataaataAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTg                                                                               <  1:1838983/93‑1 (MQ=255)

          aataaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTg                                                                               >  1:2761950/1‑93 (MQ=255)

          aataaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTg                                                                               <  2:7212457/93‑1 (MQ=255)

            taaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGc                                                                             >  2:1870408/1‑93 (MQ=255)

            taaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGc                                                                             >  1:3826070/1‑93 (MQ=255)

            taaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGc                                                                             >  1:7056000/1‑93 (MQ=255)

            taaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGc                                                                             >  2:5407754/1‑93 (MQ=255)

             aaaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCa                                                                            <  1:6788349/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  1:6370449/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  2:6489465/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  2:2390643/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  1:3343842/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  1:190662/93‑1 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCaa                                                                           >  1:120146/1‑93 (MQ=255)

              aaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCACAGTGCCGACGCCCTACTGGCaa                                                                           >  2:2354972/1‑93 (MQ=255)

              aaCAAGCCATGGCAGCGGCATTTGGTCGGGCATCCGCACCTTATGAGCAACATGCAGATCTACAGCCCCAGAGTGCCGACGCCTTACTGGCaa                                                                           <  2:5236362/93‑1 (MQ=255)

                 aaGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGc                                                                        >  2:4955973/1‑93 (MQ=255)

                  aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCt                                                                       <  1:655873/93‑1 (MQ=255)

                  aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCt                                                                       >  2:9494291/1‑93 (MQ=255)

                   gCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCtt                                                                      >  2:5065884/1‑93 (MQ=255)

                   gCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCtt                                                                      >  2:126493/1‑93 (MQ=255)

                     cATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGGGCCGACGCCTTACTGGCAATACTTcc                                                                    >  2:8859595/1‑93 (MQ=255)

                       ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCcac                                                                  >  1:9379741/1‑93 (MQ=255)

                       ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCcac                                                                  >  1:2426299/1‑93 (MQ=255)

                       ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCcac                                                                  >  2:779650/1‑93 (MQ=255)

                         gCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAg                                                                >  1:845035/1‑93 (MQ=255)

                          cAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGc                                                               >  1:8205535/1‑93 (MQ=255)

                           aGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCTAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCg                                                              >  1:9716503/1‑93 (MQ=255)

                           aGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAggg                                                              <  1:7301593/93‑3 (MQ=255)

                           aGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCg                                                              >  2:3814786/1‑93 (MQ=255)

                            gCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGt                                                             >  2:2736663/1‑93 (MQ=255)

                            gCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGt                                                             <  1:8902874/93‑1 (MQ=255)

                              ggCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTaa                                                           >  1:878235/1‑93 (MQ=255)

                              ggCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTaa                                                           >  1:5589442/1‑93 (MQ=255)

                               tcctttGGCCGGCCACCCGCCCACTATGAGAAACATGCAGATCTCCAGCGCGAGGTTGCCGCCCCTTTAGGGGCAATGCTTCCACAGCGTaaa                                                          <  2:8330058/90‑1 (MQ=255)

                               gCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTaaa                                                          >  1:7354349/1‑93 (MQ=255)

                               gCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTaaa                                                          <  2:5802756/93‑1 (MQ=255)

                                cATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAAt                                                         <  1:7228608/93‑1 (MQ=255)

                                cATTTGGGCGTGCAGCCGCTCACTATGAGCAACGTGCAGATCTACAGCGCCAGAGTGCAGACGCCTTACTGGAAATGCTTCCACAGCGTAAAt                                                         <  2:4388490/93‑1 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAc                                                                                 <  2:3648533/67‑1 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAc                                                                                 >  1:3648533/1‑67 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                       >  1:9795698/1‑93 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                       >  2:2121773/1‑93 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                       <  2:7640877/93‑1 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                       >  1:1054885/1‑93 (MQ=255)

                                  tttGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                       >  1:1382423/1‑93 (MQ=255)

                                     ggTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACAcc                                                    <  1:9189026/93‑1 (MQ=255)

                                       tCGGGCAGCCGCACACTATGAGCAACAGGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCa                                                  <  1:7254418/93‑1 (MQ=255)

                                         gggCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACg                                                >  2:6550985/1‑93 (MQ=255)

                                               ccGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTgg                                          <  1:9467816/93‑1 (MQ=255)

                                               ccGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTga                                          >  1:7498259/1‑92 (MQ=255)

                                                 gCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGAc                                        >  1:8735667/1‑93 (MQ=255)

                                                   acacTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGAcgc                                      >  1:2500071/1‑93 (MQ=255)

                                                    cacTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGAcgcg                                     >  2:7524608/1‑93 (MQ=255)

                                                     acTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCgg                                    >  1:5922451/1‑93 (MQ=255)

                                                      cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCggg                                   >  1:2252582/1‑93 (MQ=255)

                                                      cTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCggg                                   >  2:4004618/1‑93 (MQ=255)

                                                       tATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGt                                  >  1:4779983/1‑93 (MQ=255)

                                                       tATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGt                                  <  1:6932192/93‑1 (MQ=255)

                                                         tGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTtg                                <  2:1008845/93‑1 (MQ=255)

                                                          gAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTtgt                               >  1:4251014/1‑93 (MQ=255)

                                                          gAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTtgt                               <  2:6145638/93‑1 (MQ=255)

                                                           aGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTtgtg                              >  2:8502960/1‑93 (MQ=255)

                                                            gCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTgg                             >  2:9005516/1‑93 (MQ=255)

                                                            gCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTgg                             >  2:9183776/1‑93 (MQ=255)

                                                            gCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTgg                             <  1:4943720/93‑1 (MQ=255)

                                                            gCAACATGCAGATCTACAGCGCCACAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTgg                             >  1:5087086/1‑93 (MQ=255)

                                                              aaCATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGAc                           <  2:9379740/93‑1 (MQ=255)

                                                               aCATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGAcc                          >  1:4089413/1‑93 (MQ=255)

                                                               aCATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGAcc                          <  2:9724842/93‑1 (MQ=255)

                                                                cATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACct                         >  1:4018036/1‑93 (MQ=255)

                                                                cATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACct                         <  2:5740554/93‑1 (MQ=255)

                                                                  tGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACctgg                       >  2:6666807/1‑93 (MQ=255)

                                                                  tGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACctgg                       <  2:1788811/93‑1 (MQ=255)

                                                                  tGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACctgg                       >  2:5587388/1‑93 (MQ=255)

                                                                      gATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACctggctgg                   >  2:2969786/1‑93 (MQ=255)

                                                                       aTCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGa                  >  1:5639880/1‑93 (MQ=255)

                                                                        tCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGAt                 >  1:7646518/1‑93 (MQ=255)

                                                                         ctgcAGCGCCAGAGTGCCCACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATg                <  2:7989034/90‑1 (MQ=255)

                                                                         cTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATg                >  1:6000152/1‑93 (MQ=255)

                                                                             aGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAgcc            <  1:6210062/93‑1 (MQ=255)

                                                                             aGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAgcc            >  2:3587086/1‑93 (MQ=255)

                                                                              gcgcCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAgccg           >  2:4628763/1‑93 (MQ=255)

                                                                              gcgcCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAgccg           <  2:3396137/93‑1 (MQ=255)

                                                                               cgcCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAgccgc          <  1:3536712/93‑1 (MQ=255)

                                                                                 ccAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCa        <  1:7926081/93‑1 (MQ=255)

                                                                                    gagTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTg     >  2:458224/1‑93 (MQ=255)

                                                                                     agTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTgg    <  2:7862412/93‑1 (MQ=255)

                                                                                     agTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTgg    >  1:1261786/1‑93 (MQ=255)

                                                                                      gTGCCGACGCCTTACTGGCAATGCTTCCACAGAGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCAGCCCCTgca   >  1:825370/1‑91 (MQ=255)

GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCG  >  NC_000913/810747‑810926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAA  >  NC_000913/810744‑810930

catgaaaagGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCC                                                                                               <  SRR4302540.5819081/85‑1 (MQ=60)

CGTGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCC                                                                                               >  SRR4302540.10025560/1‑94 (MQ=60)

CATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCTGATCTACAGCGCCAGAGTGCC                                                                                               >  SRR4302540.10130457/1‑94 (MQ=60)

CATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCC                                                                                               <  SRR4302540.3900618/94‑1 (MQ=60)

 ATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCG                                                                                              >  SRR4302540.9854851/1‑94 (MQ=60)

 ATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCG                                                                                              >  SRR4302540.353188/1‑94 (MQ=60)

 ATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCG                                                                                              >  SRR4302540.9532533/1‑94 (MQ=60)

 ATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCG                                                                                              >  SRR4302540.8739089/1‑94 (MQ=60)

   GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGAC                                                                                            <  SRR4302540.4462333/94‑1 (MQ=60)

    GCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACG                                                                                           <  SRR4302540.10059831/94‑1 (MQ=60)

    GCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACG                                                                                           <  SRR4302540.543290/94‑1 (MQ=60)

    GCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACG                                                                                           <  SRR4302540.3031334/94‑1 (MQ=60)

      AACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCC                                                                                         >  SRR4302540.4649415/1‑94 (MQ=60)

       ACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCT                                                                                        >  SRR4302540.743061/1‑94 (MQ=60)

       ACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCT                                                                                        >  SRR4302540.2526255/1‑94 (MQ=60)

        CGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTT                                                                                       >  SRR4302540.1271043/1‑94 (MQ=60)

        CGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTT                                                                                       <  SRR4302540.6498765/94‑1 (MQ=60)

          gtaaataacaaacCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTCCAGCGCCAGAGTGCCGACGCCTTAC                                                                                     <  SRR4302540.9416569/81‑1 (MQ=60)

          GTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAC                                                                                     >  SRR4302540.4249073/1‑94 (MQ=60)

          GTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTAC                                                                                     >  SRR4302540.2358017/1‑94 (MQ=60)

            TAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTG                                                                                   >  SRR4302540.8205871/1‑94 (MQ=60)

            TAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTG                                                                                   >  SRR4302540.2791792/1‑94 (MQ=60)

             AATAAATAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGG                                                                                  <  SRR4302540.1859119/94‑1 (MQ=60)

             AATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGG                                                                                  <  SRR4302540.2428004/94‑1 (MQ=60)

             AATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGG                                                                                  <  SRR4302540.982792/94‑1 (MQ=60)

              ATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGC                                                                                 >  SRR4302540.3867705/1‑94 (MQ=60)

              ATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGC                                                                                 >  SRR4302540.7134467/1‑94 (MQ=60)

                AAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAA                                                                               <  SRR4302540.6863418/94‑1 (MQ=60)

                AAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAA                                                                               <  SRR4302540.7844734/94‑1 (MQ=60)

                AAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAA                                                                               >  SRR4302540.121438/1‑94 (MQ=60)

                 AACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAAT                                                                              <  SRR4302540.192561/94‑1 (MQ=60)

                 AACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAAT                                                                              <  SRR4302540.6441385/94‑1 (MQ=60)

                 AACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAAT                                                                              <  SRR4302540.3380531/94‑1 (MQ=60)

                  ACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATG                                                                             >  SRR4302540.3263288/1‑94 (MQ=60)

                  ACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATG                                                                             <  SRR4302540.9828167/94‑1 (MQ=60)

                     AGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTT                                                                          <  SRR4302540.662864/94‑1 (MQ=60)

                     AGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTT                                                                          >  SRR4302540.5111643/1‑94 (MQ=60)

                     AGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACACCGCCAGAGTGCCGACGCCTTACTGGCAATGCTT                                                                          >  SRR4302540.7100815/1‑94 (MQ=60)

                        CATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCA                                                                       <  SRR4302540.8898164/94‑1 (MQ=60)

                        CATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCA                                                                       <  SRR4302540.4084036/94‑1 (MQ=60)

                         ATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCAC                                                                      >  SRR4302540.2452436/1‑94 (MQ=60)

                         ATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCAC                                                                      >  SRR4302540.9491395/1‑94 (MQ=60)

                          TTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACA                                                                     <  SRR4302540.587497/94‑1 (MQ=60)

                          TTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACA                                                                     <  SRR4302540.4441003/94‑1 (MQ=60)

                           TGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAG                                                                    >  SRR4302540.854001/1‑94 (MQ=60)

                           TGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAG                                                                    >  SRR4302540.2279452/1‑94 (MQ=60)

                           TGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAG                                                                    <  SRR4302540.1695005/94‑1 (MQ=60)

                            GCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGC                                                                   <  SRR4302540.10064391/94‑1 (MQ=60)

                            GCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGC                                                                   >  SRR4302540.8300892/1‑94 (MQ=60)

                             CAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCTAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCG                                                                  >  SRR4302540.9830667/1‑94 (MQ=60)

                              AGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGGGT                                                                 <  SRR4302540.7383091/94‑1 (MQ=60)

                               GCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTA                                                                <  SRR4302540.9005945/94‑1 (MQ=60)

                                CGGCATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAA                                                               >  SRR4302540.887556/1‑94 (MQ=60)

                                CGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAA                                                               <  SRR4302540.5592228/94‑1 (MQ=60)

                                CGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAA                                                               >  SRR4302540.5650956/1‑94 (MQ=60)

                                 GGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAA                                                              >  SRR4302540.7436372/1‑94 (MQ=60)

                                   CATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATA                                                            <  SRR4302540.7309218/94‑1 (MQ=60)

                                   CATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATA                                                            <  SRR4302540.7309219/94‑1 (MQ=60)

                                   CATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCAACAGCGTAAATA                                                            >  SRR4302540.3779729/1‑94 (MQ=60)

                                    ATTTGGTCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAC                                                           <  SRR4302540.7336751/94‑1 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTaagcgctagatcggaagagcggttc                                                           >  SRR4302540.3688500/1‑69 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAC                                                           >  SRR4302540.1397180/1‑94 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAC                                                           >  SRR4302540.9910428/1‑94 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAC                                                           >  SRR4302540.1397179/1‑94 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAC                                                           >  SRR4302540.1065624/1‑94 (MQ=60)

                                    ATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATAA                                                           >  SRR4302540.9010429/1‑94 (MQ=60)

                                       TGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACC                                                        <  SRR4302540.2627189/94‑1 (MQ=60)

                                        TGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCC                                                       <  SRR4302540.2402741/94‑1 (MQ=60)

                                        GGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCC                                                       <  SRR4302540.9299223/94‑1 (MQ=60)

                                          TCGGGCAGCTGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC                                                     <  SRR4302540.8623496/94‑1 (MQ=60)

                                          TCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC                                                     <  SRR4302540.338688/94‑1 (MQ=60)

                                          TCGGGCAGCCGCACACTATGAGCAACAGGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC                                                     <  SRR4302540.7335373/94‑1 (MQ=60)

                                            GGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGT                                                   >  SRR4302540.8870584/1‑94 (MQ=60)

                                             GGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTA                                                  >  SRR4302540.8446135/1‑94 (MQ=60)

                                                AGCCGCACACTATGAGGAACATGCAGATATCCAGCGCCAGCGAGACGACGCCTTACTGGCAATGCTTCCACAGCGTCAAGACACCCACGTACTG                                               >  SRR4302540.1300466/1‑94 (MQ=53)

                                                AGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTG                                               >  SRR4302540.5376464/1‑94 (MQ=60)

                                                AGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTG                                               >  SRR4302540.1300467/1‑94 (MQ=60)

                                                 GCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGA                                              >  SRR4302540.7582037/1‑94 (MQ=60)

                                                  CCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGA                                             <  SRR4302540.6964278/94‑1 (MQ=60)

                                                  CCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGA                                             <  SRR4302540.9580129/94‑1 (MQ=60)

                                                   CGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGAC                                            >  SRR4302540.8837100/1‑94 (MQ=60)

                                                   CGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGAC                                            >  SRR4302540.77028/1‑94 (MQ=60)

                                                    GCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACG                                           >  SRR4302540.5176521/1‑94 (MQ=60)

                                                     CACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGC                                          >  SRR4302540.2253901/1‑94 (MQ=60)

                                                     CACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGC                                          >  SRR4302540.2527041/1‑94 (MQ=60)

                                                       CACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGG                                        <  SRR4302540.6582720/94‑1 (MQ=60)

                                                       CACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGG                                        >  SRR4302540.5987887/1‑94 (MQ=60)

                                                       CACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGG                                        >  SRR4302540.5987886/1‑94 (MQ=60)

                                                        ACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGG                                       >  SRR4302540.2276758/1‑94 (MQ=60)

                                                         CTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGT                                      >  SRR4302540.4831068/1‑94 (MQ=60)

                                                         CTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGT                                      >  SRR4302540.4830783/1‑94 (MQ=60)

                                                          TATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTT                                     <  SRR4302540.7009333/94‑1 (MQ=60)

                                                          TATGAGAAACATGCAGATCTACAGCGCCAGAGTGCCCACGCATTCTTGGCAATGCTTCAACAGCGTAAATACACCAACGTACTGCACGCGGCTT                                     >  SRR4302540.1895701/1‑94 (MQ=46)

                                                           ATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTG                                    <  SRR4302540.9928413/94‑1 (MQ=60)

                                                           ATGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTG                                    >  SRR4302540.5767724/1‑94 (MQ=60)

                                                            TGAGCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGT                                   >  SRR4302540.4297261/1‑94 (MQ=60)

                                                              AGCAACATGCAGATCTACAGCGCCACAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG                                 >  SRR4302540.5142087/1‑94 (MQ=60)

                                                               GCAACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGA                                <  SRR4302540.4997083/94‑1 (MQ=60)

                                                                 AACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACC                              >  SRR4302540.4133519/1‑94 (MQ=60)

                                                                  ACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCT                             <  SRR4302540.9554658/94‑1 (MQ=60)

                                                                  ACATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCT                             >  SRR4302540.4061320/1‑94 (MQ=60)

                                                                   CATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTG                            <  SRR4302540.6319992/94‑1 (MQ=60)

                                                                    ATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGG                           >  SRR4302540.742302/1‑94 (MQ=60)

                                                                    ATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGG                           >  SRR4302540.6277212/1‑94 (MQ=60)

                                                                    ATGCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACACACGTACTGGACGCGGGTTGTGGACCTGG                           >  SRR4302540.7489126/1‑94 (MQ=60)

                                                                      GCAGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCT                         <  SRR4302540.9856146/94‑1 (MQ=60)

                                                                        AGATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGG                       >  SRR4302540.3981595/1‑94 (MQ=60)

                                                                         GATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGA                      >  SRR4302540.5701957/1‑94 (MQ=60)

                                                                          ATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGAT                     <  SRR4302540.3890835/94‑1 (MQ=60)

                                                                          ATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGAT                     <  SRR4302540.5701932/94‑1 (MQ=60)

                                                                          ATCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGAT                     >  SRR4302540.7732588/1‑94 (MQ=60)

                                                                           TCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATG                    >  SRR4302540.8644836/1‑94 (MQ=60)

                                                                           TCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATG                    >  SRR4302540.6722881/1‑94 (MQ=60)

                                                                           TCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATG                    >  SRR4302540.6066652/1‑94 (MQ=60)

                                                                           TCTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATG                    >  SRR4302540.8262817/1‑94 (MQ=60)

                                                                            CTACAGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGA                   <  SRR4302540.4986625/94‑1 (MQ=60)

                                                                                tgagcgAGAGTGCCGACGCCTTACTGGCAATGCTCCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCG               <  SRR4302540.6279172/88‑1 (MQ=60)

                                                                                AGCGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCG               <  SRR4302540.6279171/94‑1 (MQ=60)

                                                                                  CGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCC             <  SRR4302540.3575328/94‑1 (MQ=60)

                                                                                  CGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCC             >  SRR4302540.9379599/1‑94 (MQ=60)

                                                                                  CGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCC             <  SRR4302540.3575329/94‑1 (MQ=60)

                                                                                  CGCCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCC             <  SRR4302540.1107651/94‑1 (MQ=60)

                                                                                    CCAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCAC           <  SRR4302540.8018465/94‑1 (MQ=60)

                                                                                     CAGAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGGTGTGGACCTGGCTGGATGAGCCGGCACT          >  SRR4302540.1904315/1‑94 (MQ=60)

                                                                                       GAGTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGG        >  SRR4302540.1274734/1‑94 (MQ=60)

                                                                                        AGTGCCGACGCCTTACTGGCAATGCTTCCACAGAGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCagcccctgca       >  SRR4302540.834150/1‑84 (MQ=60)

                                                                                         GTGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCG      >  SRR4302540.7259840/1‑94 (MQ=60)

                                                                                          TGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGG     >  SRR4302540.9715282/1‑94 (MQ=60)

                                                                                          TGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGG     >  SRR4302540.8446986/1‑94 (MQ=60)

                                                                                          TGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGG     <  SRR4302540.830544/94‑1 (MQ=60)

                                                                                          TGCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGG     <  SRR4302540.7310046/94‑1 (MQ=60)

                                                                                           GCCGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGG    <  SRR4302540.8309820/94‑1 (MQ=60)

                                                                                             CGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGA  >  SRR4302540.5819681/1‑94 (MQ=60)

CATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAA  >  NC_000913/810744‑810930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`