Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	934,839	T→C	V798A (GTT→GCT)	ftsK →	cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	934,839	0	T	C	98.4%	532.5 / ‑1.6	185	V798A (GTT→GCT)	ftsK	cell division protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (89/93); total (91/94)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

Only 100 of 211 total aligned reads displayed.

CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGGC  >  NC_000913/934749‑934930

cacaCCAGCGGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTg                                                                                           <  1:354665/93‑1 (MQ=255)

cacaACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTg                                                                                           >  2:7657735/1‑93 (MQ=255)

 acaacaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTgc                                                                                          >  1:5375297/1‑93 (MQ=255)

 acaacaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTgc                                                                                          >  2:6300746/1‑93 (MQ=255)

   accaGCCGGTGCCGCCCCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTgcgc                                                                                        <  2:8317163/91‑1 (MQ=18)

   aacaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTgcgc                                                                                        >  1:3984571/1‑93 (MQ=255)

      aGCCGGTCGCCCCTCATCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGcgccgc                                                                                     <  2:5721848/93‑1 (MQ=18)

        ccGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcag                                                                                   >  1:2003136/1‑93 (MQ=255)

           gtgccgccccAGCCACAATAGCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcag                                                                                <  2:1919275/89‑1 (MQ=18)

           gTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCCACAACCGGTTGGTCCgccgcaa                                                                                >  1:6482433/1‑92 (MQ=18)

           gTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCgcagcag                                                                                >  1:6482435/1‑93 (MQ=255)

           gTTCCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcag                                                                                <  2:4177813/93‑1 (MQ=255)

            ttGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcagc                                                                               <  1:5282731/93‑1 (MQ=255)

            gtGCGCCGCACCCACAATAGCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcagc                                                                               <  2:3242386/92‑1 (MQ=18)

              gcgcCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGcagcagcag                                                                             >  1:4193378/1‑93 (MQ=255)

              gcgcCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGcagcagcag                                                                             >  1:5717687/1‑93 (MQ=255)

                cccgcAGCCACAATATCAGCAGCCGCAACAACCGCTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTa                                                                           <  1:5550706/92‑1 (MQ=255)

                cccccAGCCACAATACCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTa                                                                           <  1:8840019/89‑1 (MQ=18)

                   gcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                        <  2:87843/93‑1 (MQ=255)

                      gCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagca                                                                     >  1:1702988/1‑93 (MQ=255)

                       ccACAGTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagcag                                                                    <  2:3948787/93‑1 (MQ=255)

                       ccACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagcag                                                                    >  2:7101034/1‑93 (MQ=255)

                        cacaATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCagcagc                                                                   >  2:2697948/1‑93 (MQ=255)

                           aaTATCGGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                                <  1:9003068/93‑1 (MQ=255)

                           aaTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                                >  1:2205984/1‑93 (MQ=255)

                           aaTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                                >  1:1006089/1‑93 (MQ=255)

                             tatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                              <  1:9458177/93‑1 (MQ=255)

                              accagcagCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCacaac                                                             <  2:3397970/91‑1 (MQ=255)

                               acaccagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaaca                                                            <  1:7529329/89‑1 (MQ=255)

                                 agcagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGc                                                          <  1:9370937/93‑1 (MQ=255)

                                  gcagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGcc                                                         >  1:1774213/1‑93 (MQ=255)

                                   cagcCGCAACAACAGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCa                                                        <  2:2117433/93‑1 (MQ=255)

                                    agcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAg                                                       <  1:226979/93‑1 (MQ=255)

                                      ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                     >  1:481179/1‑93 (MQ=255)

                                      ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                     >  1:9840262/1‑93 (MQ=255)

                                      ccGCAACAACCGGTTGCGCAACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                     <  1:4625075/93‑1 (MQ=255)

                                      ccGCAAAAACCGGTTCCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                     <  1:8307544/93‑1 (MQ=255)

                                       cGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTg                                                    >  1:2925618/1‑93 (MQ=255)

                                       cGCAAAAACCGATTGCACCACAGCAGCAATATCGACAGCCGCAACAACCGGCTGGGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTg                                                    <  1:2127760/93‑1 (MQ=14)

                                        gCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGttac                                                   >  1:6829740/1‑91 (MQ=255)

                                        gCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgc                                                   <  2:5258861/93‑1 (MQ=255)

                                           acaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgcgtc                                                >  2:5536847/1‑91 (MQ=255)

                                           acaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGcc                                                >  2:8126839/1‑93 (MQ=255)

                                             aacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGATGGGCcac                                              >  1:1640882/1‑93 (MQ=255)

                                              acgggTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                             <  1:6592597/90‑1 (MQ=255)

                                              acCGGTTGCGCCACAGCAGCAATATCAGCAGGCGCAACAACCCGCTGCGGCGCAGCAGCAGCATCACCAGCCACAACTGCCATTTGGGCcaca                                             >  2:7752032/1‑93 (MQ=17)

                                               ccGGTGGCGCCACAGCAGCAATATCAGCAGCCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACaa                                            <  2:2308922/93‑1 (MQ=255)

                                                cGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAc                                           >  2:2197260/1‑93 (MQ=255)

                                                cGGTTGCGCCACAGCACCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAc                                           <  2:2510529/93‑1 (MQ=255)

                                                 gggtGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAcc                                          <  2:3693114/90‑1 (MQ=255)

                                                  gtggcgcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                         <  2:2642482/90‑1 (MQ=255)

                                                  gTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                         >  2:9199621/1‑93 (MQ=255)

                                                   ttGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGc                                        >  1:1769794/1‑93 (MQ=255)

                                                    tGCGCCACAGCAGCAATATCAGCAGCCGCAACACCCGGCTCCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCa                                       <  1:704901/93‑1 (MQ=255)

                                                    tGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCa                                       <  1:7782427/93‑1 (MQ=255)

                                                     gcgcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAg                                      <  2:6871271/93‑1 (MQ=255)

                                                       gcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGa                                    <  1:8783187/93‑1 (MQ=255)

                                                       cccACAACAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGa                                    <  1:1019853/92‑1 (MQ=255)

                                                          acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                 >  2:4304659/1‑93 (MQ=255)

                                                          acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                                 >  1:9618514/1‑93 (MQ=255)

                                                           cagcacAAATACGAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAcc                                <  2:389746/93‑1 (MQ=21)

                                                            agcagcAATATCAGCAGCCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                               <  2:8874714/93‑1 (MQ=255)

                                                            agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                               <  1:5969895/93‑1 (MQ=255)

                                                            agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                               <  1:9505176/93‑1 (MQ=255)

                                                                   atatCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCa                        >  2:3304187/1‑93 (MQ=255)

                                                                     atCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATc                      >  1:380089/1‑93 (MQ=255)

                                                                     atCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATc                      <  2:8500279/93‑1 (MQ=255)

                                                                     atCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATc                      <  2:4686092/93‑1 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                     >  1:9205456/1‑93 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                     >  2:9235544/1‑93 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                     >  2:3218229/1‑93 (MQ=255)

                                                                      tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                     <  1:5019649/93‑1 (MQ=255)

                                                                       cagcagCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCg                    <  2:6228707/93‑1 (MQ=255)

                                                                        agcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGc                   <  2:10028478/93‑1 (MQ=255)

                                                                        agcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGc                   >  1:3201728/1‑93 (MQ=255)

                                                                        agcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTACGCCACAACCGCAGGATACCCTGCTTCATCCGc                   >  2:125298/1‑93 (MQ=255)

                                                                         gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                  >  2:3476587/1‑93 (MQ=255)

                                                                          cagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTg                 <  1:8342492/93‑1 (MQ=255)

                                                                             ccGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTg              <  1:5148366/93‑1 (MQ=255)

                                                                             ccGCAACAACCGGCTCCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTg              <  2:2524603/93‑1 (MQ=255)

                                                                              cGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa             <  2:1192385/93‑1 (MQ=255)

                                                                              cGCAACAACCGTCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa             <  1:3134177/93‑1 (MQ=255)

                                                                              cGCAACAACCGGCTTCCCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa             <  2:4396160/93‑1 (MQ=255)

                                                                              cGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa             <  1:7151534/93‑1 (MQ=255)

                                                                              cGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa             <  2:2994205/93‑1 (MQ=255)

                                                                               gCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt            >  2:2414354/1‑93 (MQ=255)

                                                                               gCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt            >  2:80003/1‑93 (MQ=255)

                                                                               gCAACAACCGGATGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt            <  2:9946138/93‑1 (MQ=255)

                                                                               caaacaacCGGCTGCGCCGCCGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt            <  1:4341923/91‑1 (MQ=255)

                                                                                  acaacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCg         >  1:471214/1‑93 (MQ=255)

                                                                                    aacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa       <  2:6697669/93‑1 (MQ=255)

                                                                                    aacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa       >  1:7963557/1‑93 (MQ=255)

                                                                                    aacCGGCGGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa       <  1:2828166/93‑1 (MQ=255)

                                                                                     acCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTaa      >  2:7489529/1‑93 (MQ=255)

                                                                                     acCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTaa      <  2:5989437/93‑1 (MQ=255)

                                                                                      ccGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAAt     <  1:5867115/93‑1 (MQ=255)

                                                                                      ccGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAAt     <  1:8703293/93‑1 (MQ=255)

                                                                                      acGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAAt     <  1:4171020/92‑1 (MQ=255)

                                                                                       cGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATg    <  2:6092990/93‑1 (MQ=255)

                                                                                         gTTCCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATCCCCTGCTTCATCCGCTGTTGATGCGTAATGGc  <  2:6816639/93‑1 (MQ=255)

CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGGC  >  NC_000913/934749‑934930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGGCG  >  NC_000913/934746‑934931

AGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGC                                                                                              <  SRR4302540.7621099/94‑1 (MQ=60)

AGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGC                                                                                              >  SRR4302540.1707567/1‑94 (MQ=60)

ACCCACACCAGCGGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGC                                                                                              <  SRR4302540.1105985/94‑1 (MQ=60)

 GCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCT                                                                                             <  SRR4302540.5108133/94‑1 (MQ=60)

 CCCACAACAGCTGGTGGCGCCGCAGCCACAAGAACAGCAGCCGCAACAACCGGGTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCT                                                                                             <  SRR4302540.2084023/94‑1 (MQ=49)

   CACACCAGCGGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGC                                                                                           <  SRR4302540.358304/94‑1 (MQ=60)

   CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGC                                                                                           >  SRR4302540.8908418/1‑94 (MQ=60)

   CACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGC                                                                                           >  SRR4302540.5434398/1‑94 (MQ=60)

     CAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGC                                                                                         >  SRR4302540.4027479/1‑94 (MQ=60)

         AGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCA                                                                                     >  SRR4302540.1757729/1‑94 (MQ=60)

         AGCCGGTTCCGCCGCAGCAACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCA                                                                                     <  SRR4302540.9730557/94‑1 (MQ=60)

          GCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAG                                                                                    >  SRR4302540.2024821/1‑94 (MQ=60)

            CGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCA                                                                                  >  SRR4302540.5494857/1‑94 (MQ=60)

            CGGTGGCGCCCCAGCCACAAAATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCA                                                                                  <  SRR4302540.4635531/94‑1 (MQ=60)

             GGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCCACAACCGGTTGgtccgccgcaa                                                                                 >  SRR4302540.6554537/1‑83 (MQ=60)

             GGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAG                                                                                 >  SRR4302540.6554539/1‑94 (MQ=60)

             GGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAG                                                                                 >  SRR4302540.6554538/1‑94 (MQ=60)

             GGTTCCCCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAG                                                                                 <  SRR4302540.8267062/94‑1 (MQ=60)

               TTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCA                                                                               <  SRR4302540.5340445/94‑1 (MQ=60)

                TGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAG                                                                              >  SRR4302540.5780620/1‑94 (MQ=60)

                TGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAG                                                                              >  SRR4302540.4238996/1‑94 (MQ=60)

                TGCGCCCCAGCCACAAAAGCAGAAGCCGCAACAGCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAG                                                                              <  SRR4302540.3553609/94‑1 (MQ=48)

                   ccccCAGCCACAATACCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAT                                                                           <  SRR4302540.8942267/90‑1 (MQ=60)

                   GCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAT                                                                           >  SRR4302540.4036356/1‑94 (MQ=60)

                   GCCGAAGCCACAATAGCACCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAT                                                                           <  SRR4302540.2189282/94‑1 (MQ=60)

                   CCCGCAGCCACAATATCAGCAGCCGCAACAACCGCTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAT                                                                           <  SRR4302540.5611851/94‑1 (MQ=60)

                     CGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCA                                                                         >  SRR4302540.6172067/1‑94 (MQ=60)

                       CAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGC                                                                       >  SRR4302540.2230369/1‑94 (MQ=60)

                        AGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCA                                                                      <  SRR4302540.4318669/94‑1 (MQ=60)

                        AGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCA                                                                      >  SRR4302540.1721416/1‑94 (MQ=60)

                        AGCCACAATACCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCA                                                                      <  SRR4302540.7283885/94‑1 (MQ=60)

                         GCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAG                                                                     >  SRR4302540.1661674/1‑94 (MQ=60)

                            ACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCA                                                                  >  SRR4302540.6875751/1‑94 (MQ=60)

                             CAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCAC                                                                 >  SRR4302540.1016293/1‑94 (MQ=60)

                             CAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCAC                                                                 >  SRR4302540.2229816/1‑94 (MQ=60)

                              AATATCGGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACA                                                                <  SRR4302540.9111081/94‑1 (MQ=60)

                                TATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAAC                                                              <  SRR4302540.9570410/94‑1 (MQ=60)

                                TAACAGCAGCCGCAACAACCGGTTGCACCCCGGCAGCAATATCGGCAGCCGCATCAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAAC                                                              <  SRR4302540.2821993/94‑1 (MQ=18)

                                 ATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACA                                                             >  SRR4302540.1838974/1‑94 (MQ=60)

                                  acacCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAG                                                            <  SRR4302540.7613609/90‑1 (MQ=60)

                                   CAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGC                                                           >  SRR4302540.9614595/1‑94 (MQ=60)

                                    AGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCC                                                          >  SRR4302540.8463262/1‑94 (MQ=60)

                                    AGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCC                                                          >  SRR4302540.1793476/1‑94 (MQ=60)

                                    AGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCC                                                          >  SRR4302540.5659140/1‑94 (MQ=60)

                                    AGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCC                                                          >  SRR4302540.1793477/1‑94 (MQ=60)

                                    AGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCC                                                          <  SRR4302540.9482508/94‑1 (MQ=60)

                                       AGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGT                                                       <  SRR4302540.229171/94‑1 (MQ=60)

                                        GCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTT                                                      >  SRR4302540.486334/1‑94 (MQ=60)

                                        GCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTT                                                      >  SRR4302540.9955307/1‑94 (MQ=60)

                                        GCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTT                                                      <  SRR4302540.2926979/94‑1 (MQ=60)

                                         cgcCAACACCCGGTTCCGCCACAGCGCCAATAACAGCTGCAGCAACAACCGGCTACGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTG                                                     <  SRR4302540.6561423/91‑1 (MQ=30)

                                         CCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTG                                                     <  SRR4302540.8404000/94‑1 (MQ=60)

                                         CCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTG                                                     >  SRR4302540.2957686/1‑94 (MQ=60)

                                         CCGCAACAACCGGTTGCGCAACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTG                                                     <  SRR4302540.4674635/94‑1 (MQ=60)

                                         CCGCAAAAACCGGTTCCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTG                                                     <  SRR4302540.8403999/94‑1 (MQ=60)

                                          CGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGC                                                    >  SRR4302540.5340084/1‑94 (MQ=60)

                                          CGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTAC                                                    >  SRR4302540.6905361/1‑94 (MQ=60)

                                          CGCAAAAACCGATTGCACCACAGCAGCAATATCGACAGCCGCAACAACCGGCTGGGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGC                                                    <  SRR4302540.2150589/94‑1 (MQ=39)

                                              ACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCA                                                >  SRR4302540.905675/1‑94 (MQ=60)

                                              ACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCA                                                >  SRR4302540.7779713/1‑94 (MQ=60)

                                               CAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCAC                                               >  SRR4302540.4362245/1‑94 (MQ=60)

                                               CAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGATGGGCCAC                                               >  SRR4302540.1658814/1‑94 (MQ=60)

                                                 ACGGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAA                                             <  SRR4302540.6665606/94‑1 (MQ=60)

                                                  CCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAC                                            >  SRR4302540.9137277/1‑94 (MQ=60)

                                                   CGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGAAGCCACAACAGCCAGTTGCGCCACAACC                                           >  SRR4302540.2663212/1‑94 (MQ=60)

                                                    GGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCG                                          <  SRR4302540.5954367/94‑1 (MQ=60)

                                                    GGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCG                                          <  SRR4302540.6662938/94‑1 (MQ=60)

                                                     GTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGC                                         >  SRR4302540.4726504/1‑94 (MQ=60)

                                                     GTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGC                                         >  SRR4302540.1788996/1‑94 (MQ=60)

                                                      TTGCGCCACAGCAGCAATATCAGCAGCCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCA                                        <  SRR4302540.2062434/94‑1 (MQ=60)

                                                       TGCGCCACAGCAGCAATATCAGCAGCCGCAACACCCGGCTCCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAG                                       <  SRR4302540.712468/94‑1 (MQ=60)

                                                       TGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAG                                       <  SRR4302540.7873395/94‑1 (MQ=60)

                                                          GCCACAGCCGGAATATCAGCAGCCCCAGCAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    <  SRR4302540.1030204/94‑1 (MQ=60)

                                                          GCCACAGCAGCAATCTCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    <  SRR4302540.9270773/94‑1 (MQ=60)

                                                          GCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    >  SRR4302540.3865047/1‑94 (MQ=60)

                                                          GCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    <  SRR4302540.8884857/94‑1 (MQ=60)

                                                          GCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGCATCAGCAGCCACAACAGCCAGTTGCGGCACAACCGCAGGga                                    >  SRR4302540.3083566/1‑92 (MQ=60)

                                                          CCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    <  SRR4302540.5919832/94‑1 (MQ=60)

                                                          CCCACAACAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGAT                                    <  SRR4302540.1030205/94‑1 (MQ=60)

                                                           CCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATA                                   <  SRR4302540.488232/94‑1 (MQ=60)

                                                            CACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAC                                  >  SRR4302540.9731902/1‑94 (MQ=60)

                                                             ACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACC                                 <  SRR4302540.9320893/94‑1 (MQ=60)

                                                              CAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCC                                <  SRR4302540.5420838/94‑1 (MQ=60)

                                                               AGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCT                               <  SRR4302540.6035970/94‑1 (MQ=60)

                                                               AGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCT                               <  SRR4302540.9617791/94‑1 (MQ=60)

                                                                      ATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCAT                        >  SRR4302540.9858612/1‑94 (MQ=60)

                                                                      ATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCAT                        <  SRR4302540.1144137/94‑1 (MQ=60)

                                                                       TATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATC                       >  SRR4302540.384023/1‑94 (MQ=60)

                                                                        ATCAGCAGCCGCAACACCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCC                      <  SRR4302540.4231131/94‑1 (MQ=60)

                                                                        ATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCC                      >  SRR4302540.9315914/1‑94 (MQ=60)

                                                                        ATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCC                      <  SRR4302540.3114104/94‑1 (MQ=60)

                                                                        ATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCC                      <  SRR4302540.5637178/94‑1 (MQ=60)

                                                                         TCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCG                     <  SRR4302540.5073989/94‑1 (MQ=60)

                                                                          CAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGC                    >  SRR4302540.3236497/1‑94 (MQ=60)

                                                                           AGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCT                   <  SRR4302540.7254680/94‑1 (MQ=60)

                                                                             CAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGT                 <  SRR4302540.8439322/94‑1 (MQ=60)

                                                                              CGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTT                <  SRR4302540.8846317/94‑1 (MQ=60)

                                                                              AGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTT                >  SRR4302540.4815470/1‑94 (MQ=60)

                                                                                CCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGA              <  SRR4302540.5204165/94‑1 (MQ=60)

                                                                                CCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGA              <  SRR4302540.947104/94‑1 (MQ=60)

                                                                                 CGCAACAACCGTCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.3168368/94‑1 (MQ=60)

                                                                                 CGCAACAACCGGCTTCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.7103156/94‑1 (MQ=60)

                                                                                 CGCAACAACCGGCTGCGCCGCAGCAGCAGTATTAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             >  SRR4302540.8024031/1‑94 (MQ=60)

                                                                                 CGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.7199969/94‑1 (MQ=60)

                                                                                 CGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.2752453/94‑1 (MQ=60)

                                                                                 CGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.7231229/94‑1 (MQ=60)

                                                                                 CGCAACAACCGGCTGCGCCGCAGCAGCAGGATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAT             <  SRR4302540.3212977/94‑1 (MQ=60)

                                                                                  caAACAACCGGCTGCGCCGCCGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATG            <  SRR4302540.4389186/92‑1 (MQ=60)

                                                                                   CAACAACGGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGC           <  SRR4302540.375921/94‑1 (MQ=60)

                                                                                    AACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCG          >  SRR4302540.476264/1‑94 (MQ=60)

                                                                                     ACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGT         >  SRR4302540.5172574/1‑94 (MQ=60)

                                                                                      CAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTA        >  SRR4302540.8056368/1‑94 (MQ=60)

                                                                                       AACCGGCGGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAA       <  SRR4302540.2858749/94‑1 (MQ=60)

                                                                                         CCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATG     <  SRR4302540.8804322/94‑1 (MQ=60)

                                                                                         CCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATG     <  SRR4302540.5931980/94‑1 (MQ=60)

                                                                                         CCGGCGGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATG     <  SRR4302540.7967388/94‑1 (MQ=60)

                                                                                          CGGCTGCGCCGCAGCAGCAGTATCGGCAGCGACAACAGCCAGGTGGGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGGAATGG    >  SRR4302540.8921815/1‑94 (MQ=60)

                                                                                          CGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATTCGTAATGG    >  SRR4302540.2028086/1‑94 (MQ=60)

                                                                                          CGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGG    >  SRR4302540.8921817/1‑94 (MQ=60)

                                                                                            GCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGGCG  <  SRR4302540.4949526/94‑1 (MQ=60)

AGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATGGCG  >  NC_000913/934746‑934931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`