Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 968,384 T→C S266P (TCT→CCT)  ycaH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913968,3840TC98.2% 857.5 / 1.5 271S266P (TCT→CCT) ycaHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (135/131);  total (137/134)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 274 total aligned reads displayed.

CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTT  >  NC_000913/968295‑968473
                                                                                         |                                                                                         
cGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCtt                                                                                        <  1:4140020/93‑1 (MQ=255)
 ggggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCttt                                                                                       >  1:1541012/1‑93 (MQ=255)
 ggggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCttt                                                                                       >  1:9545891/1‑93 (MQ=255)
 ggggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCttt                                                                                       >  2:4497010/1‑93 (MQ=255)
  gggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTg                                                                                      <  1:1909149/93‑1 (MQ=255)
  gggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTg                                                                                      >  1:7359620/1‑93 (MQ=255)
    gATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGaa                                                                                    >  2:8485689/1‑93 (MQ=255)
       tGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCa                                                                                 <  2:9229111/93‑1 (MQ=255)
       tGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCa                                                                                 <  1:8822403/93‑1 (MQ=255)
       tGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCa                                                                                 >  1:834917/1‑93 (MQ=255)
           cgtCCGCCGCGCTGTTTTGCCACGCTGAAGAGGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCg                                                                             <  1:753929/91‑1 (MQ=255)
           cATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCg                                                                             >  2:8501428/1‑93 (MQ=255)
            aTCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCgg                                                                            >  2:7955105/1‑93 (MQ=255)
            aTCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCgg                                                                            <  2:8686246/93‑1 (MQ=255)
             tCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACGACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGa                                                                           >  1:4962149/1‑93 (MQ=255)
             tCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGa                                                                           <  2:2056778/93‑1 (MQ=255)
             tCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGa                                                                           >  1:5915231/1‑93 (MQ=255)
              ccgccgCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGAt                                                                          >  2:8036969/1‑93 (MQ=255)
                 ccggGCTTTTTTGCCACGCTGAAGATGTGTGGCGTAAAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTc                                                                       <  2:8472957/93‑1 (MQ=255)
                 ccgcGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTc                                                                       >  2:2077758/1‑93 (MQ=255)
                 ccgcGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTc                                                                       <  2:1421905/93‑1 (MQ=255)
                   gcgcTTTTTTGCCACGCTGAAGATGTGTGGCGTACAAACGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAg                                                                     >  2:8892465/1‑93 (MQ=255)
                       ttttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCg                                                                 <  1:1702539/93‑1 (MQ=255)
                       ttttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCg                                                                 >  1:7769480/1‑93 (MQ=255)
                        tttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGt                                                                <  1:323619/93‑1 (MQ=255)
                        tttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGt                                                                >  1:6888811/1‑93 (MQ=255)
                         ttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCGGCCTTTGAACCATGCGGATGTCAGTGCGtt                                                               >  1:6582419/1‑93 (MQ=255)
                         ttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGtt                                                               <  2:6226296/93‑1 (MQ=255)
                         ttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGtt                                                               >  2:7649208/1‑93 (MQ=255)
                         ttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGtt                                                               <  2:3404353/93‑1 (MQ=255)
                            tGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGt                                                            >  2:8996005/1‑93 (MQ=255)
                            tGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGt                                                            >  1:567289/1‑93 (MQ=255)
                              ccACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTaa                                                          >  1:638879/1‑93 (MQ=255)
                                aCGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAgc                                                        >  1:7045638/1‑93 (MQ=255)
                                  gCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAgcgc                                                      >  1:952238/1‑93 (MQ=255)
                                   cTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGcc                                                     <  1:351862/93‑1 (MQ=255)
                                    tGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTCCGCGCCg                                                    >  2:1283042/1‑93 (MQ=255)
                                    tGAAGATGTGCGGCGTACAACCGGAAAACCGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATTTCAGCGCGCTGGCACGCGTCg                                                    >  1:3038089/1‑93 (MQ=255)
                                     gAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCgg                                                   <  1:386236/93‑1 (MQ=255)
                                       aGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGc                                                 <  2:7340265/93‑1 (MQ=255)
                                         aTGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCaa                                               >  1:8248836/1‑93 (MQ=255)
                                         aTGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCaa                                               <  2:1603589/93‑1 (MQ=255)
                                           gtgtgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAc                                             <  1:1149722/93‑1 (MQ=255)
                                           gtgtgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAc                                             <  1:6230036/93‑1 (MQ=255)
                                              tgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCt                                          <  2:4716522/93‑1 (MQ=255)
                                              tgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCt                                          <  1:92242/93‑1 (MQ=255)
                                              tgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCt                                          >  2:6546008/1‑93 (MQ=255)
                                              tgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGAGGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCt                                          >  2:5112273/1‑93 (MQ=255)
                                               ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg                                         >  1:972756/1‑93 (MQ=255)
                                               ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg                                         <  2:6438423/93‑1 (MQ=255)
                                               ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg                                         <  2:8197671/93‑1 (MQ=255)
                                               ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg                                         >  1:5222710/1‑93 (MQ=255)
                                               ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg                                         <  2:5265248/93‑1 (MQ=255)
                                                gCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg                                        <  2:8271807/93‑1 (MQ=255)
                                                  gTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTa                                      >  1:5589177/1‑93 (MQ=255)
                                                  gTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTa                                      >  2:2974205/1‑93 (MQ=255)
                                                  gTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTa                                      >  1:7679763/1‑93 (MQ=255)
                                                  gTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTa                                      <  1:8427040/93‑1 (MQ=255)
                                                   tACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTaa                                     <  1:2444202/93‑1 (MQ=255)
                                                   tACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTaa                                     <  2:4694939/93‑1 (MQ=255)
                                                    aCAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAt                                    <  2:5563086/93‑1 (MQ=255)
                                                     cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATg                                   <  2:7138815/93‑1 (MQ=255)
                                                     cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATg                                   <  2:5128813/93‑1 (MQ=255)
                                                      aaCCGGAAAAATGTGTACCGCTGGCCTATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGa                                  <  2:2246772/93‑1 (MQ=255)
                                                      aaCCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGa                                  <  1:8557672/93‑1 (MQ=255)
                                                       aCCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGAc                                 <  2:5151368/93‑1 (MQ=255)
                                                       aCCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGAc                                 <  1:8624995/93‑1 (MQ=255)
                                                       aCCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGAc                                 <  2:1717810/93‑1 (MQ=255)
                                                             aaaaTGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGaaaa                           >  1:8237984/1‑93 (MQ=255)
                                                                aTGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCATACGCTGGTAATGACTGAACAAGa                        >  2:3189829/1‑93 (MQ=255)
                                                                aTGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGa                        <  2:5875809/93‑1 (MQ=255)
                                                                aTGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGa                        <  1:4811651/93‑1 (MQ=255)
                                                                 tgtgTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAt                       >  2:3797180/1‑93 (MQ=255)
                                                                  gtgtACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                      >  2:9690593/1‑93 (MQ=255)
                                                                  gtgtACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                      <  1:7599570/93‑1 (MQ=255)
                                                                  gtgtACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                      <  1:5360029/93‑1 (MQ=255)
                                                                  gtgtACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                      <  1:3696670/93‑1 (MQ=255)
                                                                   tgtACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGc                     <  1:4432895/93‑1 (MQ=255)
                                                                      aCCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGt                  <  2:5917687/93‑1 (MQ=255)
                                                                        cGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGa                >  1:9307564/1‑93 (MQ=255)
                                                                         gCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaa               <  2:3579496/93‑1 (MQ=255)
                                                                          cTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaaa              <  1:614679/93‑1 (MQ=255)
                                                                          cTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaaa              <  2:2641479/93‑1 (MQ=255)
                                                                          cTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaaa              <  2:7137244/93‑1 (MQ=255)
                                                                           tGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAt             >  1:5153338/1‑93 (MQ=255)
                                                                           tGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAt             >  1:9073239/1‑93 (MQ=255)
                                                                            ggCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATg            <  1:4108343/93‑1 (MQ=255)
                                                                            ggCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATg            >  2:4271455/1‑93 (MQ=255)
                                                                             gCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGc           <  2:3871670/93‑1 (MQ=255)
                                                                             gCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGc           >  1:1268725/1‑93 (MQ=255)
                                                                                gATCATCGGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCgg        <  1:8941364/93‑1 (MQ=255)
                                                                                gATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCgg        >  1:801242/1‑93 (MQ=255)
                                                                                gATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCgg        >  1:5708539/1‑93 (MQ=255)
                                                                                  tcatcaGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGc      >  2:5640560/1‑93 (MQ=255)
                                                                                  tcatcaGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGc      <  1:1013395/93‑1 (MQ=255)
                                                                                     tcaGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCtt   <  1:7888296/93‑1 (MQ=255)
                                                                                     tcaGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCtt   >  2:6239234/1‑93 (MQ=255)
                                                                                     tcaGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCtt   >  2:4022830/1‑93 (MQ=255)
                                                                                      caGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCttt  <  1:399606/93‑1 (MQ=255)
                                                                                      caGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCttt  <  1:2892503/93‑1 (MQ=255)
                                                                                         |                                                                                         
CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTT  >  NC_000913/968295‑968473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAG  >  NC_000913/968291‑968477
                                                                                             |                                                                                             
ATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGC                                                                                               <  SRR4302540.1938152/94‑1 (MQ=60)
    CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTT                                                                                           >  SRR4302540.9658830/1‑94 (MQ=60)
    CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTT                                                                                           >  SRR4302540.9658829/1‑94 (MQ=60)
    CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTT                                                                                           <  SRR4302540.4184819/94‑1 (MQ=60)
    CGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTT                                                                                           >  SRR4302540.1558041/1‑94 (MQ=60)
     GGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTG                                                                                          <  SRR4302540.9703214/94‑1 (MQ=60)
     GGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTG                                                                                          >  SRR4302540.7441690/1‑94 (MQ=60)
     GGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGCGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTG                                                                                          >  SRR4302540.9156120/1‑94 (MQ=60)
     GGAGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTG                                                                                          <  SRR4302540.3787880/94‑1 (MQ=60)
      GGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGA                                                                                         <  SRR4302540.1930006/94‑1 (MQ=60)
       GGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAA                                                                                        >  SRR4302540.4934233/1‑94 (MQ=60)
         GTTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACC                                                                                      <  SRR4302540.8150658/94‑1 (MQ=60)
          TTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCA                                                                                     >  SRR4302540.843784/1‑94 (MQ=60)
           TGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCAT                                                                                    <  SRR4302540.8924418/94‑1 (MQ=60)
           TGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCAT                                                                                    >  SRR4302540.7672753/1‑94 (MQ=60)
               CGTCCGCCGCGCTGTTTTGCCACGCTGAAGAGGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGG                                                                                <  SRR4302540.761965/94‑1 (MQ=60)
                ATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACGACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGA                                                                               >  SRR4302540.5015809/1‑94 (MQ=60)
                ATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGA                                                                               >  SRR4302540.5980579/1‑94 (MQ=60)
                ATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGA                                                                               >  SRR4302540.4601409/1‑94 (MQ=60)
                ATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGA                                                                               >  SRR4302540.8384125/1‑94 (MQ=60)
                   CGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGT                                                                            <  SRR4302540.2149553/94‑1 (MQ=60)
                   CGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGT                                                                            >  SRR4302540.5260854/1‑94 (MQ=60)
                          CTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCG                                                                     >  SRR4302540.7860314/1‑94 (MQ=60)
                          CTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCG                                                                     <  SRR4302540.5044163/94‑1 (MQ=60)
                          CTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTATACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCG                                                                     >  SRR4302540.5065766/1‑94 (MQ=60)
                           TTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGT                                                                    >  SRR4302540.9101909/1‑94 (MQ=60)
                           TTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGT                                                                    >  SRR4302540.6965196/1‑94 (MQ=60)
                           TTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGT                                                                    >  SRR4302540.8885303/1‑94 (MQ=60)
                           TTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGT                                                                    <  SRR4302540.1720965/94‑1 (MQ=60)
                            TTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCGGCCTTTGAACCATGCGGATGTCAGTGCGTT                                                                   >  SRR4302540.6655332/1‑94 (MQ=60)
                            TTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTT                                                                   <  SRR4302540.326861/94‑1 (MQ=60)
                            TTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTT                                                                   >  SRR4302540.7275004/1‑94 (MQ=60)
                            TTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTT                                                                   >  SRR4302540.7873039/1‑94 (MQ=60)
                               TTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGT                                                                >  SRR4302540.886893/1‑94 (MQ=60)
                               TTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGT                                                                >  SRR4302540.573454/1‑94 (MQ=60)
                                 GCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAA                                                              >  SRR4302540.645675/1‑94 (MQ=60)
                                  CCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAG                                                             <  SRR4302540.9008666/94‑1 (MQ=60)
                                  CCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAG                                                             <  SRR4302540.8376490/94‑1 (MQ=60)
                                  CCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAG                                                             <  SRR4302540.3027278/94‑1 (MQ=60)
                                   CACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGC                                                            >  SRR4302540.7123985/1‑94 (MQ=60)
                                   CACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGC                                                            >  SRR4302540.5999118/1‑94 (MQ=60)
                                    ACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCG                                                           <  SRR4302540.6483864/94‑1 (MQ=60)
                                    ACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCG                                                           <  SRR4302540.2111510/94‑1 (MQ=60)
                                     CGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGC                                                          >  SRR4302540.962128/1‑94 (MQ=60)
                                      GCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCC                                                         <  SRR4302540.799906/94‑1 (MQ=60)
                                      GCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCC                                                         >  SRR4302540.3099848/1‑94 (MQ=60)
                                       CTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCG                                                        <  SRR4302540.9342286/94‑1 (MQ=60)
                                       CTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCG                                                        <  SRR4302540.355470/94‑1 (MQ=60)
                                       CTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCG                                                        >  SRR4302540.6406670/1‑94 (MQ=60)
                                       CTGAAGATGTGCGGCGTACAACCGGAAAACCGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATttcagcgcgctggcacgcgtcg                                                        >  SRR4302540.3071434/1‑72 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATTATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGG                                                       <  SRR4302540.7816698/94‑1 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTCAGCGCCGG                                                       >  SRR4302540.8456099/1‑94 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGG                                                       >  SRR4302540.5463726/1‑94 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGG                                                       >  SRR4302540.9190560/1‑94 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGG                                                       >  SRR4302540.6746991/1‑94 (MQ=60)
                                        TGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGG                                                       <  SRR4302540.7943835/94‑1 (MQ=60)
                                         GAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGG                                                      <  SRR4302540.390230/94‑1 (MQ=60)
                                           AGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCA                                                    <  SRR4302540.6441046/94‑1 (MQ=60)
                                           AGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCA                                                    >  SRR4302540.833110/1‑94 (MQ=60)
                                            GATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAA                                                   >  SRR4302540.8344721/1‑94 (MQ=60)
                                             ATGTGTGGCGTACATCCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAA                                                  <  SRR4302540.1705282/94‑1 (MQ=60)
                                             ATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAA                                                  >  SRR4302540.9385194/1‑94 (MQ=60)
                                              TGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAC                                                 <  SRR4302540.720341/94‑1 (MQ=60)
                                              TGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAC                                                 <  SRR4302540.1098434/94‑1 (MQ=60)
                                              TGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAC                                                 <  SRR4302540.2530584/94‑1 (MQ=60)
                                              TGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAC                                                 <  SRR4302540.3587585/94‑1 (MQ=60)
                                               GTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACG                                                <  SRR4302540.1161708/94‑1 (MQ=60)
                                               GTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACG                                                <  SRR4302540.6299323/94‑1 (MQ=60)
                                                 GTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCT                                              <  SRR4302540.570212/94‑1 (MQ=60)
                                                 GTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCG                                              >  SRR4302540.4701499/1‑94 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             >  SRR4302540.982757/1‑94 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             >  SRR4302540.4041119/1‑94 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             >  SRR4302540.4041120/1‑94 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             <  SRR4302540.5804135/94‑1 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             >  SRR4302540.5279656/1‑94 (MQ=60)
                                                  TGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTG                                             <  SRR4302540.93215/94‑1 (MQ=60)
                                                    GCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGT                                           >  SRR4302540.7516013/1‑94 (MQ=60)
                                                     CGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTA                                          >  SRR4302540.7766676/1‑94 (MQ=60)
                                                     CGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTA                                          >  SRR4302540.5650686/1‑94 (MQ=60)
                                                      GTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAA                                         <  SRR4302540.8524869/94‑1 (MQ=60)
                                                      GTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAA                                         >  SRR4302540.8491133/1‑94 (MQ=60)
                                                       TACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAT                                        >  SRR4302540.582671/1‑94 (MQ=60)
                                                       TACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAT                                        <  SRR4302540.2470525/94‑1 (MQ=60)
                                                       TACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAT                                        >  SRR4302540.8101574/1‑94 (MQ=60)
                                                       TACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAT                                        <  SRR4302540.6148613/94‑1 (MQ=60)
                                                         CAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGA                                      >  SRR4302540.5622989/1‑94 (MQ=60)
                                                         CAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGA                                      <  SRR4302540.4175145/94‑1 (MQ=60)
                                                          AACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGAC                                     <  SRR4302540.3576214/94‑1 (MQ=60)
                                                          AACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGAC                                     <  SRR4302540.8657072/94‑1 (MQ=60)
                                                           ACCGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACT                                    <  SRR4302540.8725186/94‑1 (MQ=60)
                                                             CGGAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGA                                  >  SRR4302540.9221108/1‑94 (MQ=60)
                                                               GAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAA                                >  SRR4302540.6270493/1‑94 (MQ=60)
                                                               GAAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAA                                >  SRR4302540.4172662/1‑94 (MQ=60)
                                                                AAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAA                               >  SRR4302540.8333725/1‑94 (MQ=60)
                                                                AAAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAA                               >  SRR4302540.8333724/1‑94 (MQ=60)
                                                                 AAAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAA                              <  SRR4302540.8692044/94‑1 (MQ=60)
                                                                  AAATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAG                             <  SRR4302540.4283469/94‑1 (MQ=60)
                                                                   AATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGA                            <  SRR4302540.6883076/94‑1 (MQ=60)
                                                                    ATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAT                           >  SRR4302540.7496613/1‑94 (MQ=60)
                                                                    ATGTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAT                           <  SRR4302540.4863151/94‑1 (MQ=60)
                                                                      GTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGC                         <  SRR4302540.3737028/94‑1 (MQ=60)
                                                                      GTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGC                         >  SRR4302540.8645425/1‑94 (MQ=60)
                                                                      GTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGC                         <  SRR4302540.5418906/94‑1 (MQ=60)
                                                                      GTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGC                         <  SRR4302540.7684961/94‑1 (MQ=60)
                                                                      GTGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGC                         <  SRR4302540.1732531/94‑1 (MQ=60)
                                                                       TGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCG                        <  SRR4302540.4480924/94‑1 (MQ=60)
                                                                       TGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCG                        <  SRR4302540.4963387/94‑1 (MQ=60)
                                                                       TGTACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCG                        >  SRR4302540.650891/1‑94 (MQ=60)
                                                                         TACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGT                      <  SRR4302540.3953276/94‑1 (MQ=60)
                                                                         TACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGT                      <  SRR4302540.7645520/94‑1 (MQ=60)
                                                                         GACCGCTGGCCGATCGTCAGCCTTTGACCCATGCGGATGGCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGT                      <  SRR4302540.1334683/94‑1 (MQ=60)
                                                                         GACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGT                      <  SRR4302540.1334684/94‑1 (MQ=60)
                                                                          ACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGGCTGAAAAAGATGCGGTG                     <  SRR4302540.1176462/94‑1 (MQ=60)
                                                                          ACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTG                     >  SRR4302540.1798875/1‑94 (MQ=60)
                                                                          ACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTG                     <  SRR4302540.957828/94‑1 (MQ=60)
                                                                          ACCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTG                     >  SRR4302540.3247368/1‑94 (MQ=60)
                                                                           CCGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGA                    >  SRR4302540.9418700/1‑94 (MQ=60)
                                                                            CGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTTGTAATGACTGAAAAAGATGCGGTGAA                   >  SRR4302540.7080551/1‑94 (MQ=60)
                                                                            CGCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGTA                   >  SRR4302540.7738055/1‑94 (MQ=60)
                                                                             GCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAA                  >  SRR4302540.8688885/1‑94 (MQ=60)
                                                                             GCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAA                  >  SRR4302540.5400783/1‑94 (MQ=60)
                                                                             GCTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAA                  <  SRR4302540.3512506/94‑1 (MQ=60)
                                                                              CTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAT                 >  SRR4302540.1254106/1‑94 (MQ=60)
                                                                              CTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAT                 >  SRR4302540.3999935/1‑94 (MQ=60)
                                                                              CTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAT                 >  SRR4302540.5209261/1‑94 (MQ=60)
                                                                              CTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAT                 <  SRR4302540.621239/94‑1 (MQ=60)
                                                                              CTGGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAT                 >  SRR4302540.9182197/1‑94 (MQ=60)
                                                                                GGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGC               >  SRR4302540.1281777/1‑94 (MQ=60)
                                                                                GGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGC               <  SRR4302540.4152681/94‑1 (MQ=60)
                                                                                GGCCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGC               >  SRR4302540.8968452/1‑94 (MQ=60)
                                                                                  CCGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCG             <  SRR4302540.3398004/94‑1 (MQ=60)
                                                                                   TGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGG            >  SRR4302540.2493493/1‑94 (MQ=60)
                                                                                   CGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGG            >  SRR4302540.9726323/1‑94 (MQ=60)
                                                                                   CGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGG            >  SRR4302540.5771382/1‑94 (MQ=60)
                                                                                   CGATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGG            >  SRR4302540.809751/1‑94 (MQ=60)
                                                                                    GATCATCGGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGG           <  SRR4302540.9046318/94‑1 (MQ=60)
                                                                                    GATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGG           >  SRR4302540.5346451/1‑94 (MQ=60)
                                                                                     ATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGC          >  SRR4302540.5758145/1‑94 (MQ=60)
                                                                                     ATCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGC          >  SRR4302540.7822881/1‑94 (MQ=60)
                                                                                      TCATCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCC         <  SRR4302540.1023670/94‑1 (MQ=60)
                                                                                         TCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTT      <  SRR4302540.570707/94‑1 (MQ=60)
                                                                                         TCAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTT      <  SRR4302540.7980372/94‑1 (MQ=60)
                                                                                          CAGGCTTTGTACCATGCGGATGTCAGTGCGTTGGTAAGCGCCAGGCAAACGCTAGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     <  SRR4302540.9012797/94‑1 (MQ=60)
                                                                                          CAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     <  SRR4302540.403764/94‑1 (MQ=60)
                                                                                          CAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     <  SRR4302540.9541604/94‑1 (MQ=60)
                                                                                          CAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     >  SRR4302540.4372508/1‑94 (MQ=60)
                                                                                          CAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     <  SRR4302540.2924038/94‑1 (MQ=60)
                                                                                          CAGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTG     >  SRR4302540.4372507/1‑94 (MQ=60)
                                                                                           AGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGC    <  SRR4302540.1935022/94‑1 (MQ=60)
                                                                                           AGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGC    >  SRR4302540.4633063/1‑94 (MQ=60)
                                                                                           AGCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGC    >  SRR4302540.1829283/1‑94 (MQ=60)
                                                                                           AGCCTTTGAACCATGCAGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGC    >  SRR4302540.1829282/1‑94 (MQ=60)
                                                                                            GCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCA   <  SRR4302540.9879103/94‑1 (MQ=60)
                                                                                            GCCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCA   <  SRR4302540.5492876/94‑1 (MQ=60)
                                                                                             CCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAG  >  SRR4302540.680229/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAG  >  NC_000913/968291‑968477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑