Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,798	T→C	L396P (CTC→CCC)	ycaQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,798	0	T	C	99.2%	822.6 / NA	257	L396P (CTC→CCC)	ycaQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (129/125); minor base G (0/2); total (129/128)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

CGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTG  >  NC_000913/969708‑969887

cGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCccct                                                                                         >  2:7435510/1‑93 (MQ=255)

   tGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTa                                                                                      <  2:6025264/93‑1 (MQ=255)

    gCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTAc                                                                                     <  1:9229353/93‑1 (MQ=255)

       aaaaGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGa                                                                                  >  2:4626337/1‑93 (MQ=255)

        aaaGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAt                                                                                 >  2:6815588/1‑93 (MQ=255)

        aaaGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAt                                                                                 >  2:4093650/1‑93 (MQ=255)

        aaaGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAt                                                                                 >  1:7995685/1‑93 (MQ=255)

           gggCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGc                                                                              <  1:3545959/93‑1 (MQ=255)

             gCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGGTGATGCCCGCAAGACCCCTTTACTGATTGCCg                                                                            >  2:5141982/1‑93 (MQ=255)

             gCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCg                                                                            <  1:3656739/93‑1 (MQ=255)

               tACGTCAGGCGTTTACTGATTTCGCTAACTGGCAGCAGGCAACTCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTAATGACTGCCGCa                                                                          >  2:6167299/1‑93 (MQ=255)

                 cGTCAGGCGATTACTGATTTCGCTAACTGGCAGCATGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCAcc                                                                        >  2:1803549/1‑93 (MQ=255)

                   tCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCgg                                                                      >  1:1182/1‑93 (MQ=255)

                   tCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCgg                                                                      <  2:1499712/93‑1 (MQ=255)

                    cAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGc                                                                     >  2:3764755/1‑93 (MQ=255)

                    cAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGc                                                                     <  2:7304957/93‑1 (MQ=255)

                     aGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCt                                                                    <  1:4244490/93‑1 (MQ=255)

                     aGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCt                                                                    >  2:6520292/1‑93 (MQ=255)

                     aGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCt                                                                    <  2:5764607/93‑1 (MQ=255)

                     aGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCt                                                                    <  2:9158509/93‑1 (MQ=255)

                      ggCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTg                                                                   >  1:7482748/1‑93 (MQ=255)

                       gCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTgg                                                                  <  2:8124009/93‑1 (MQ=255)

                          aTTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGGCGCGCCGGCTGGGaa                                                               >  2:8350556/1‑93 (MQ=255)

                          aTTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGaa                                                               <  1:1313056/93‑1 (MQ=255)

                            tACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAAt                                                             >  1:8217224/1‑93 (MQ=255)

                            tACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAAt                                                             >  1:7877944/1‑93 (MQ=255)

                             aCTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATa                                                            <  1:723239/93‑1 (MQ=255)

                               tGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGa                                                          >  2:2748085/1‑93 (MQ=255)

                               tGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGa                                                          <  2:9733874/93‑1 (MQ=255)

                                  tttCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGAccc                                                       >  2:1111296/1‑93 (MQ=255)

                                    tCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCg                                                     <  2:9526068/93‑1 (MQ=255)

                                       cTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCg                                                  >  1:4655690/1‑93 (MQ=255)

                                       cTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATACACCCCGTCg                                                  >  1:8496556/1‑93 (MQ=255)

                                        tAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGc                                                 >  2:2633195/1‑93 (MQ=255)

                                         aaCTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTGCTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCa                                                >  1:6340470/1‑93 (MQ=255)

                                         aaCTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCa                                                >  1:4957207/1‑93 (MQ=255)

                                          aCTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCAt                                               >  1:5381735/1‑93 (MQ=255)

                                           cTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATa                                              >  1:3900814/1‑93 (MQ=255)

                                            tGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATaa                                             >  1:3065289/1‑93 (MQ=255)

                                            cggCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATaa                                             <  1:2074422/92‑1 (MQ=255)

                                             ggCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATaaa                                            >  1:6916939/1‑93 (MQ=255)

                                              gcagcaGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAt                                           >  1:5055486/1‑93 (MQ=255)

                                              gcagcaGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAt                                           <  2:346752/93‑1 (MQ=255)

                                              gcagcaGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAt                                           <  1:344719/93‑1 (MQ=255)

                                               cagcagGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATg                                          <  1:5434969/93‑1 (MQ=255)

                                                  cagGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGAtt                                       >  2:9848047/1‑93 (MQ=255)

                                                   agGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTa                                      <  2:7791141/93‑1 (MQ=255)

                                                    ggCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTAt                                     <  1:4387854/93‑1 (MQ=255)

                                                     gCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATg                                    >  1:1620463/1‑93 (MQ=255)

                                                      cAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGa                                   <  2:8828008/93‑1 (MQ=255)

                                                       aaCGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCTCATAAATGATTATGAt                                  <  2:10044832/93‑1 (MQ=255)

                                                       aaCGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGaa                                  >  2:2057981/1‑92 (MQ=255)

                                                       aaCGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAt                                  >  2:6535919/1‑93 (MQ=255)

                                                         cgcgGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATaa                                <  2:4998872/93‑1 (MQ=255)

                                                           cgGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGa                              <  2:5546986/93‑1 (MQ=255)

                                                            gagTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAt                             <  2:1594642/91‑1 (MQ=255)

                                                             ggTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAtt                            >  2:8272432/1‑93 (MQ=255)

                                                             ggTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAtt                            <  2:4619830/93‑1 (MQ=255)

                                                              gTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGGCCCCGTCGCATAAATGATTATGATAAGAttt                           >  2:2874890/1‑93 (MQ=255)

                                                              gTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAttt                           <  2:8573274/93‑1 (MQ=255)

                                                               tGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTa                          <  2:4107447/93‑1 (MQ=255)

                                                               tGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCACACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAtttt                          >  1:7915299/1‑92 (MQ=255)

                                                                gACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTAt                         >  2:2547352/1‑93 (MQ=255)

                                                                 aCATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATg                        <  1:5426366/93‑1 (MQ=255)

                                                                   cttAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCa                      <  1:8086744/92‑1 (MQ=255)

                                                                    ttAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAt                     <  2:3064641/93‑1 (MQ=255)

                                                                    ttAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAt                     >  2:8050538/1‑93 (MQ=255)

                                                                    ttAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAt                     >  1:9424690/1‑93 (MQ=255)

                                                                     tAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAtt                    <  1:4054170/93‑1 (MQ=255)

                                                                     tAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAtt                    <  1:6238882/93‑1 (MQ=255)

                                                                     tAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAtt                    <  1:654935/93‑1 (MQ=255)

                                                                      aGGATGCTGCCCGCAAGGCCGCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTc                   <  1:1021257/93‑1 (MQ=255)

                                                                      aGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTc                   <  2:5251960/93‑1 (MQ=255)

                                                                       ggATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCa                  >  1:4325999/1‑93 (MQ=255)

                                                                       ggATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCa                  <  1:950407/93‑1 (MQ=255)

                                                                        gATGCTGCCCGCAAGGCCCCTTTACTGATTGCCTCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAt                 <  2:2140662/93‑1 (MQ=255)

                                                                        gATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAt                 >  2:2109735/1‑93 (MQ=255)

                                                                        gATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAt                 <  2:4208357/93‑1 (MQ=255)

                                                                        gATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAt                 >  2:761481/1‑93 (MQ=255)

                                                                         aTGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATa                <  1:2836330/93‑1 (MQ=255)

                                                                         aTGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATa                >  1:6822611/1‑93 (MQ=255)

                                                                         aTGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATa                <  1:7162458/93‑1 (MQ=255)

                                                                          tgctgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAtca               >  1:9196980/1‑91 (MQ=255)

                                                                           gctgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGCCCCCGTCGCATAAATGATTATGATAAGATTTATGACTTCATAAc              >  1:8320277/1‑93 (MQ=255)

                                                                           gctgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAAc              >  2:4019025/1‑93 (MQ=255)

                                                                            ctgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAAcc             <  1:5806678/93‑1 (MQ=255)

                                                                             tgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAAccc            <  2:6762972/93‑1 (MQ=255)

                                                                             cgcCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGGCCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAcccc            <  2:8838712/92‑1 (MQ=255)

                                                                               cccGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGa          <  1:240080/93‑1 (MQ=255)

                                                                               cccGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGa          >  2:4733117/1‑93 (MQ=255)

                                                                                ccGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATCAATGATTATGATAAGATTTATGCATTCATCACACgct         >  2:946298/1‑91 (MQ=255)

                                                                                ccGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATAATAAGATTTATGCATTCATAACCCGAt         <  2:33174/93‑1 (MQ=255)

                                                                                 cGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATc        >  2:694906/1‑93 (MQ=255)

                                                                                   cAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCa      >  2:8483725/1‑93 (MQ=255)

                                                                                    aaGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCAt     <  1:5198627/93‑1 (MQ=255)

                                                                                     aGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATc    >  2:3824600/1‑93 (MQ=255)

                                                                                     aGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACACCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATc    >  2:2328373/1‑93 (MQ=255)

                                                                                      ggCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCt   <  1:4017333/93‑1 (MQ=255)

                                                                                      ggCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCt   >  2:6384006/1‑93 (MQ=255)

                                                                                      ggCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCt   <  2:4843014/93‑1 (MQ=255)

CGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTG  >  NC_000913/969708‑969887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAG  >  NC_000913/969705‑969890

CAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCC                                                                                              >  SRR4302540.64267/1‑94 (MQ=60)

 AACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCC                                                                                             >  SRR4302540.4266755/1‑94 (MQ=60)

  ACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCT                                                                                            <  SRR4302540.10110987/94‑1 (MQ=60)

   CGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTT                                                                                           <  SRR4302540.6505695/94‑1 (MQ=60)

   CGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTT                                                                                           >  SRR4302540.2160726/1‑94 (MQ=60)

    GCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTT                                                                                          <  SRR4302540.5381488/94‑1 (MQ=60)

      TGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTAC                                                                                        >  SRR4302540.8646688/1‑94 (MQ=60)

       GCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACT                                                                                       <  SRR4302540.9339989/94‑1 (MQ=60)

          AAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAT                                                                                    >  SRR4302540.7303414/1‑94 (MQ=60)

          AAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAT                                                                                    <  SRR4302540.7499795/94‑1 (MQ=60)

          AAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGAT                                                                                    >  SRR4302540.8088760/1‑94 (MQ=60)

           AAAGGGCTACGTCAGGCGATTACTGATTTCACTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATT                                                                                   >  SRR4302540.5338546/1‑94 (MQ=60)

            AAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTG                                                                                  <  SRR4302540.4147655/94‑1 (MQ=60)

              GGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCC                                                                                <  SRR4302540.3584684/94‑1 (MQ=60)

              GCGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCC                                                                                >  SRR4302540.7388473/1‑94 (MQ=60)

               GGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCG                                                                               <  SRR4302540.3785202/94‑1 (MQ=60)

               GGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCG                                                                               >  SRR4302540.3485616/1‑94 (MQ=60)

                GCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGC                                                                              <  SRR4302540.3696784/94‑1 (MQ=60)

                GCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGC                                                                              >  SRR4302540.7740561/1‑94 (MQ=60)

                  TACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCAC                                                                            <  SRR4302540.4803005/94‑1 (MQ=60)

                   TCGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACC                                                                           <  SRR4302540.9556078/94‑1 (MQ=60)

                    CGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCG                                                                          >  SRR4302540.59866/1‑94 (MQ=60)

                    CGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCG                                                                          >  SRR4302540.3523043/1‑94 (MQ=60)

                     GTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGG                                                                         >  SRR4302540.1191/1‑94 (MQ=60)

                     GTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGG                                                                         >  SRR4302540.5480820/1‑94 (MQ=60)

                     GTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCACCTTTACTCATTACCGCACCGC                                                                         >  SRR4302540.5480819/1‑94 (MQ=60)

                       CAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCT                                                                       <  SRR4302540.5492796/94‑1 (MQ=60)

                        AGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCCCCGGCTG                                                                      >  SRR4302540.4417521/1‑94 (MQ=60)

                        AGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTG                                                                      <  SRR4302540.4290671/94‑1 (MQ=60)

                        AGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTG                                                                      >  SRR4302540.7566272/1‑94 (MQ=60)

                        AGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTG                                                                      >  SRR4302540.8056669/1‑94 (MQ=60)

                         GGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGG                                                                     <  SRR4302540.1146770/94‑1 (MQ=60)

                           CGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGA                                                                   <  SRR4302540.5800738/94‑1 (MQ=60)

                             ATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAA                                                                 >  SRR4302540.1202707/1‑94 (MQ=60)

                             ATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAA                                                                 <  SRR4302540.1326702/94‑1 (MQ=60)

                              TTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAAT                                                                <  SRR4302540.2113305/94‑1 (MQ=60)

                              TTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAAT                                                                >  SRR4302540.7969953/1‑94 (MQ=60)

                              TTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAAT                                                                >  SRR4302540.8312733/1‑94 (MQ=60)

                                ACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAG                                                              <  SRR4302540.730991/94‑1 (MQ=60)

                                 CTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGA                                                             >  SRR4302540.3879460/1‑94 (MQ=60)

                                   GATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACC                                                           >  SRR4302540.7276266/1‑94 (MQ=60)

                                    ATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCC                                                          <  SRR4302540.9922420/94‑1 (MQ=60)

                                         GCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCG                                                     >  SRR4302540.4705514/1‑94 (MQ=60)

                                         GCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATACACCCCGTCG                                                     >  SRR4302540.8595282/1‑94 (MQ=60)

                                          CTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGAACCCGTCGC                                                    >  SRR4302540.7011444/1‑94 (MQ=60)

                                           TAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTGCTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCA                                                   >  SRR4302540.6411081/1‑94 (MQ=60)

                                           TAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCA                                                   >  SRR4302540.5236254/1‑94 (MQ=60)

                                           TAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCA                                                   >  SRR4302540.5010780/1‑94 (MQ=60)

                                            AACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCAT                                                  >  SRR4302540.5440926/1‑94 (MQ=60)

                                             ACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATA                                                 >  SRR4302540.3943135/1‑94 (MQ=60)

                                              CTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAA                                                >  SRR4302540.3098919/1‑94 (MQ=60)

                                               TGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAA                                               >  SRR4302540.6993906/1‑94 (MQ=60)

                                               CGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAA                                               <  SRR4302540.2096726/94‑1 (MQ=60)

                                                GGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAT                                              >  SRR4302540.5110142/1‑94 (MQ=60)

                                                 GCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATG                                             <  SRR4302540.2825987/94‑1 (MQ=60)

                                                 GCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATG                                             <  SRR4302540.348221/94‑1 (MQ=60)

                                                  CAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGA                                            <  SRR4302540.5494746/94‑1 (MQ=60)

                                                   AGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGAT                                           >  SRR4302540.1221623/1‑94 (MQ=60)

                                                     CAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTA                                         >  SRR4302540.258191/1‑94 (MQ=60)

                                                      AGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCGCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTAT                                        <  SRR4302540.5250575/94‑1 (MQ=60)

                                                      AGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTAT                                        >  SRR4302540.812252/1‑94 (MQ=60)

                                                      AGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTAT                                        <  SRR4302540.193064/94‑1 (MQ=60)

                                                       GGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATG                                       >  SRR4302540.1638226/1‑94 (MQ=60)

                                                       GGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATG                                       <  SRR4302540.4435481/94‑1 (MQ=60)

                                                       GGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATG                                       >  SRR4302540.1638227/1‑94 (MQ=60)

                                                         CAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAT                                     <  SRR4302540.6654202/94‑1 (MQ=60)

                                                         CAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAT                                     >  SRR4302540.49152/1‑94 (MQ=60)

                                                         CAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCACTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAT                                     <  SRR4302540.8002445/94‑1 (MQ=60)

                                                          AACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATA                                    >  SRR4302540.4422079/1‑94 (MQ=60)

                                                              CGGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGAT                                <  SRR4302540.812990/94‑1 (MQ=60)

                                                               GGGTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATT                               <  SRR4302540.4449588/94‑1 (MQ=60)

                                                                 GTGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCACACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTT                             >  SRR4302540.8007575/1‑94 (MQ=60)

                                                                  TGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTAT                            >  SRR4302540.8029801/1‑94 (MQ=60)

                                                                  GGACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTAT                            <  SRR4302540.8777065/94‑1 (MQ=60)

                                                                    ACATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGC                          <  SRR4302540.5486045/94‑1 (MQ=60)

                                                                     CATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCA                         >  SRR4302540.2938448/1‑94 (MQ=60)

                                                                     CATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCA                         >  SRR4302540.8254348/1‑94 (MQ=60)

                                                                      CTTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAT                        <  SRR4302540.8180609/94‑1 (MQ=60)

                                                                      ATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAT                        >  SRR4302540.9536683/1‑94 (MQ=60)

                                                                      ATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAT                        <  SRR4302540.9460022/94‑1 (MQ=60)

                                                                      ATTAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCAT                        >  SRR4302540.8484996/1‑94 (MQ=60)

                                                                       ATAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATT                       <  SRR4302540.5826938/94‑1 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      <  SRR4302540.661913/94‑1 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      <  SRR4302540.6308299/94‑1 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      >  SRR4302540.1599793/1‑94 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      <  SRR4302540.6308298/94‑1 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      <  SRR4302540.4097877/94‑1 (MQ=60)

                                                                        TAGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTC                      <  SRR4302540.4097878/94‑1 (MQ=60)

                                                                         AGGATGCTGCCCGCAAGGCCGCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCA                     <  SRR4302540.1031618/94‑1 (MQ=60)

                                                                         AGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCA                     >  SRR4302540.4373098/1‑94 (MQ=60)

                                                                         AGGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCA                     >  SRR4302540.4372806/1‑94 (MQ=60)

                                                                          GGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAT                    >  SRR4302540.395867/1‑94 (MQ=60)

                                                                          GGATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCAT                    <  SRR4302540.960288/94‑1 (MQ=60)

                                                                           GATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATA                   <  SRR4302540.2959733/94‑1 (MQ=60)

                                                                           GATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATA                   >  SRR4302540.6898141/1‑94 (MQ=60)

                                                                           GATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATA                   <  SRR4302540.685930/94‑1 (MQ=60)

                                                                           GATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATA                   >  SRR4302540.9561386/1‑94 (MQ=60)

                                                                           GATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATA                   <  SRR4302540.4159294/94‑1 (MQ=60)

                                                                            ATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATCA                  >  SRR4302540.9307303/1‑94 (MQ=60)

                                                                            ATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAA                  <  SRR4302540.362995/94‑1 (MQ=60)

                                                                            ATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAA                  <  SRR4302540.6933409/94‑1 (MQ=60)

                                                                            ATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAA                  <  SRR4302540.7242308/94‑1 (MQ=60)

                                                                            ATGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAA                  <  SRR4302540.2867008/94‑1 (MQ=60)

                                                                             TGCTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGCCCCCGTCGCATAAATGATTATGATAAGATTTATGACTTCATAAC                 >  SRR4302540.8416856/1‑94 (MQ=60)

                                                                               CTGCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCC               <  SRR4302540.5870841/94‑1 (MQ=60)

                                                                                 GCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGA             >  SRR4302540.9343106/1‑94 (MQ=60)

                                                                                 GCCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGA             <  SRR4302540.6949562/94‑1 (MQ=60)

                                                                                  CCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGAT            <  SRR4302540.242404/94‑1 (MQ=60)

                                                                                  CCCGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAAACCGAT            >  SRR4302540.4584581/1‑94 (MQ=60)

                                                                                    CGCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCC          >  SRR4302540.7659471/1‑94 (MQ=60)

                                                                                     GCAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCA         >  SRR4302540.3198434/1‑94 (MQ=60)

                                                                                      CAAGGCCGCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCAT        <  SRR4302540.3609054/94‑1 (MQ=60)

                                                                                      CAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCAT        <  SRR4302540.8497292/94‑1 (MQ=60)

                                                                                      CAAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCAT        >  SRR4302540.6507341/1‑94 (MQ=60)

                                                                                       AAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATC       >  SRR4302540.9322243/1‑94 (MQ=60)

                                                                                       AAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATC       <  SRR4302540.5255235/94‑1 (MQ=60)

                                                                                       AAGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATCATAAGATTTATGAATTCATAAACCGATCCATC       >  SRR4302540.4064584/1‑94 (MQ=60)

                                                                                        AGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCT      <  SRR4302540.5062516/94‑1 (MQ=60)

                                                                                        AGGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCT      <  SRR4302540.8293290/94‑1 (MQ=60)

                                                                                         GGCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTG     <  SRR4302540.4060609/94‑1 (MQ=60)

                                                                                          GCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGG    <  SRR4302540.6159157/94‑1 (MQ=60)

                                                                                          GCCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGG    <  SRR4302540.8388434/94‑1 (MQ=60)

                                                                                           CCCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGG   >  SRR4302540.7223671/1‑94 (MQ=60)

                                                                                            CCCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAG  >  SRR4302540.7831763/1‑94 (MQ=60)

CAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAG  >  NC_000913/969705‑969890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`