Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 974,364 A→G I275V (ATT→GTT)  mukF → MukF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913974,3640AG99.6% 796.3 / ‑5.7 248I275V (ATT→GTT) mukFMukF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (149/98);  minor base T (0/1);  total (149/99)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 261 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453
                                                                                          |                                                                                         
 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        >  1:8071030/1‑93 (MQ=255)
 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        >  1:1386707/1‑93 (MQ=255)
 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        >  2:9049161/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTTGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                       >  2:9214122/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTTGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                       >  2:3969677/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                       >  2:6209693/1‑93 (MQ=255)
     gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTa                                                                                    <  2:5322958/93‑1 (MQ=255)
     gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTa                                                                                    >  2:6277896/1‑93 (MQ=255)
       aTTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACg                                                                                  >  2:6171603/1‑93 (MQ=255)
        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                 <  1:9586042/93‑1 (MQ=255)
        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                 >  1:5385512/1‑93 (MQ=255)
         ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                >  1:5470782/1‑93 (MQ=255)
            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                             >  1:1659576/1‑93 (MQ=255)
                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATGGACTTGTGGGTTGGCTACGGCGGCCAct                                                                         >  1:3008140/1‑92 (MQ=255)
                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                         >  1:5062781/1‑93 (MQ=255)
                  tgTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTa                                                                       <  2:856917/92‑1 (MQ=255)
                   gTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTac                                                                      >  1:6287037/1‑93 (MQ=255)
                   gTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTac                                                                      >  1:4355626/1‑93 (MQ=255)
                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                     >  2:6748915/1‑93 (MQ=255)
                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAACGCCACGTaca                                                                     >  2:5258755/1‑93 (MQ=255)
                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGGTGGCTACGACCGCCACGTaca                                                                     >  1:7692986/1‑93 (MQ=255)
                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGGCTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                   <  1:7386427/93‑1 (MQ=255)
                        gtgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACaaa                                                                 <  2:5796271/93‑1 (MQ=255)
                         tgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAt                                                                >  2:548839/1‑93 (MQ=255)
                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              <  2:7515425/93‑1 (MQ=255)
                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              >  2:1403429/1‑93 (MQ=255)
                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          <  1:7811317/93‑1 (MQ=255)
                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          <  1:4158760/93‑1 (MQ=255)
                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          >  1:1200422/1‑93 (MQ=255)
                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACTACCGCCACGTACACAAATTTATTCg                                                         >  1:6022667/1‑93 (MQ=255)
                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         >  2:8727540/1‑93 (MQ=255)
                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  1:3762544/1‑93 (MQ=255)
                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       <  1:980387/93‑1 (MQ=255)
                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                      >  1:3821651/1‑93 (MQ=255)
                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                      >  1:847975/1‑93 (MQ=255)
                                    cAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAcc                                                     >  1:271130/1‑93 (MQ=255)
                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                    <  1:2916841/93‑1 (MQ=255)
                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  1:7216288/1‑93 (MQ=255)
                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  2:4746884/1‑93 (MQ=255)
                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   <  2:8448118/93‑1 (MQ=255)
                                       agCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgcg                                                  >  1:1535012/1‑93 (MQ=255)
                                       agCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgcg                                                  >  2:7721050/1‑93 (MQ=255)
                                        gCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcga                                                 >  2:3423115/1‑93 (MQ=255)
                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                <  2:9479300/93‑1 (MQ=255)
                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                >  1:3509916/1‑93 (MQ=255)
                                           aaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcg                                              >  2:2839637/1‑93 (MQ=255)
                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           <  1:4135387/93‑1 (MQ=255)
                                               cGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGatat                                          <  2:7540797/93‑1 (MQ=255)
                                               cGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGatat                                          <  1:8981344/93‑1 (MQ=255)
                                               cGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTGGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGatat                                          <  2:4212439/93‑1 (MQ=255)
                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         <  1:9444221/93‑1 (MQ=255)
                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                        <  1:2361165/93‑1 (MQ=255)
                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                       <  1:1764676/93‑1 (MQ=255)
                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                      <  2:1615426/93‑1 (MQ=255)
                                                    gTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATa                                     >  2:3457371/1‑93 (MQ=255)
                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  2:6035594/1‑93 (MQ=255)
                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  1:4414518/1‑93 (MQ=255)
                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  1:3360706/1‑93 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACGGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                   >  1:6579230/1‑93 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                   >  2:2202926/1‑93 (MQ=255)
                                                       ttatCAGTTGGGGCCAGCAATCCATCGGCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  <  1:2771075/93‑1 (MQ=255)
                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  <  2:2400100/93‑1 (MQ=255)
                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  2:2134289/1‑93 (MQ=255)
                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  1:2200815/1‑93 (MQ=255)
                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  2:1078546/1‑93 (MQ=255)
                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                <  1:8228108/93‑1 (MQ=255)
                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                               >  1:8654435/1‑93 (MQ=255)
                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                            >  1:6067405/1‑93 (MQ=255)
                                                               tGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTc                          <  1:7829458/93‑1 (MQ=255)
                                                                ggggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                         <  2:2742361/93‑1 (MQ=255)
                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       <  1:8693007/93‑1 (MQ=255)
                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       >  2:4063896/1‑93 (MQ=255)
                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       <  2:8038048/93‑1 (MQ=255)
                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGc                     <  1:7764227/93‑1 (MQ=255)
                                                                     cAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCt                    >  2:5209706/1‑93 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                   >  2:7155653/1‑93 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                   <  2:7646748/93‑1 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                   >  1:4549944/1‑93 (MQ=255)
                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                  >  1:6939866/1‑93 (MQ=255)
                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAgt                >  2:9891729/1‑92 (MQ=255)
                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                >  2:64948/1‑93 (MQ=255)
                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               >  1:2107150/1‑93 (MQ=255)
                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              >  1:5861743/1‑93 (MQ=255)
                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              >  2:88413/1‑93 (MQ=255)
                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt             >  2:2527672/1‑93 (MQ=255)
                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt            <  2:3604171/93‑1 (MQ=255)
                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt            >  2:1055750/1‑93 (MQ=255)
                                                                             cATCGACTTGTGGGGTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt            >  2:5955363/1‑93 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           >  2:381652/1‑93 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           <  2:3192327/93‑1 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           <  2:1149453/93‑1 (MQ=255)
                                                                               tCGAGTGGTGGGGTGGCTGCGACCGCCACGTAAACAAATTGATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAc          <  1:2732547/93‑1 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       >  1:4802711/1‑93 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  1:1341388/93‑1 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  1:257324/93‑1 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  1:2589143/93‑1 (MQ=255)
                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  2:1923655/1‑93 (MQ=255)
                                                                                    ttGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAg     >  1:9234730/1‑93 (MQ=255)
                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt    >  1:7683351/1‑93 (MQ=255)
                                                                                       tgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCg  >  1:1007665/1‑93 (MQ=255)
                                                                                          |                                                                                         
GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  >  NC_000913/974273‑974457
                                                                                           |                                                                                             
CGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTT                                                                                             <  SRR4302540.6927883/94‑1 (MQ=60)
CGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTT                                                                                             >  SRR4302540.8521049/1‑94 (MQ=60)
CGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTT                                                                                             >  SRR4302540.9020367/1‑94 (MQ=60)
 GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            <  SRR4302540.5112085/94‑1 (MQ=60)
 GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            >  SRR4302540.1867152/1‑94 (MQ=60)
 GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            >  SRR4302540.8164673/1‑94 (MQ=60)
 GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            >  SRR4302540.1401537/1‑94 (MQ=60)
  ATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGG                                                                                           >  SRR4302540.358789/1‑94 (MQ=60)
   TCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGC                                                                                          <  SRR4302540.765715/94‑1 (MQ=60)
    CTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCT                                                                                         <  SRR4302540.1540634/94‑1 (MQ=60)
        ATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGA                                                                                     >  SRR4302540.5444745/1‑94 (MQ=60)
        ATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGA                                                                                     >  SRR4302540.7391473/1‑94 (MQ=60)
        ATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGCGGGTTGGCTACGA                                                                                     >  SRR4302540.1542791/1‑94 (MQ=60)
         TTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAC                                                                                    <  SRR4302540.8694188/94‑1 (MQ=60)
         TTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAC                                                                                    >  SRR4302540.5531005/1‑94 (MQ=60)
         TTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAC                                                                                    <  SRR4302540.9699210/94‑1 (MQ=60)
            TGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGC                                                                                 <  SRR4302540.6243112/94‑1 (MQ=60)
            CGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGC                                                                                 >  SRR4302540.1677656/1‑94 (MQ=60)
             GTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCTACTTGTGGGTTGGCTACGACCGCC                                                                                >  SRR4302540.4410658/1‑94 (MQ=60)
              TCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCA                                                                               <  SRR4302540.9518328/94‑1 (MQ=60)
                GATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATGGACTTGTGGGTTGGCTACGgcggccact                                                                             >  SRR4302540.3041248/1‑85 (MQ=60)
                GATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACG                                                                             >  SRR4302540.5117518/1‑94 (MQ=60)
                 ATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGT                                                                            <  SRR4302540.9067858/94‑1 (MQ=60)
                  TCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTA                                                                           >  SRR4302540.7880307/1‑94 (MQ=60)
                   CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          >  SRR4302540.2165857/1‑94 (MQ=60)
                   CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          >  SRR4302540.4403001/1‑94 (MQ=60)
                   CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          >  SRR4302540.6357015/1‑94 (MQ=60)
                    GTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGGTGGCTACGACCGCCACGTACA                                                                         >  SRR4302540.7780535/1‑94 (MQ=60)
                      CTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACA                                                                       <  SRR4302540.4213612/94‑1 (MQ=60)
                       TGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGGCTTGTGGGTTGGCTACGACCGCCACGTACACAA                                                                      <  SRR4302540.7468725/94‑1 (MQ=60)
                       TGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAA                                                                      >  SRR4302540.6093304/1‑94 (MQ=60)
                          TGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATT                                                                   <  SRR4302540.3754822/94‑1 (MQ=60)
                          TGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATT                                                                   <  SRR4302540.8686265/94‑1 (MQ=60)
                          GGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATT                                                                   <  SRR4302540.1112460/94‑1 (MQ=60)
                           GTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTT                                                                  <  SRR4302540.1250150/94‑1 (MQ=60)
                            TTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTA                                                                 >  SRR4302540.382444/1‑94 (MQ=60)
                               GATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTC                                                              >  SRR4302540.960754/1‑94 (MQ=60)
                               GATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTC                                                              >  SRR4302540.1212769/1‑94 (MQ=60)
                               GATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTC                                                              <  SRR4302540.4200734/94‑1 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACTACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.6089484/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACTACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.6089483/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.5295158/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             <  SRR4302540.4203827/94‑1 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.2783215/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.2684276/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.2684275/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             <  SRR4302540.7902601/94‑1 (MQ=60)
                                ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302540.229909/1‑94 (MQ=60)
                                ATCTGCAGAGCAAACGCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCCCCACGTACACAAATTTATTCG                                                             <  SRR4302540.4203826/94‑1 (MQ=60)
                                 TCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGT                                                            <  SRR4302540.9472233/94‑1 (MQ=60)
                                  CTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTA                                                           >  SRR4302540.3803591/1‑94 (MQ=60)
                                   TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          <  SRR4302540.8055665/94‑1 (MQ=60)
                                   TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          >  SRR4302540.3863245/1‑94 (MQ=60)
                                   TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          >  SRR4302540.856969/1‑94 (MQ=60)
                                   TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          <  SRR4302540.990426/94‑1 (MQ=60)
                                    GCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACC                                                         <  SRR4302540.7432744/94‑1 (MQ=60)
                                    GCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACC                                                         >  SRR4302540.273802/1‑94 (MQ=60)
                                      AGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGC                                                       <  SRR4302540.2948760/94‑1 (MQ=60)
                                      AGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGC                                                       >  SRR4302540.4557862/1‑94 (MQ=60)
                                      AGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGC                                                       >  SRR4302540.7296725/1‑94 (MQ=60)
                                       GAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCG                                                      >  SRR4302540.5078704/1‑94 (MQ=60)
                                       GAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCG                                                      >  SRR4302540.1551995/1‑94 (MQ=60)
                                        AGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGA                                                     >  SRR4302540.4040352/1‑94 (MQ=60)
                                        AGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGA                                                     <  SRR4302540.7944917/94‑1 (MQ=60)
                                         GCAAGCACGATCTTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGAT                                                    <  SRR4302540.840149/94‑1 (MQ=60)
                                         GCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGAT                                                    >  SRR4302540.3548145/1‑94 (MQ=60)
                                           aaccgCGATCGTTTTTTCAGTTGGGGCCAGCTATCCATGGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCG                                                  <  SRR4302540.7381337/89‑1 (MQ=60)
                                           AAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCG                                                  >  SRR4302540.633235/1‑94 (MQ=60)
                                             ACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGAT                                                <  SRR4302540.4520950/94‑1 (MQ=60)
                                               TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              <  SRR4302540.4180129/94‑1 (MQ=60)
                                               TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              <  SRR4302540.7909086/94‑1 (MQ=60)
                                               TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              <  SRR4302540.3474831/94‑1 (MQ=60)
                                                CGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATG                                             <  SRR4302540.9088417/94‑1 (MQ=60)
                                                CGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATG                                             <  SRR4302540.7808737/94‑1 (MQ=60)
                                                 GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGG                                            <  SRR4302540.9556343/94‑1 (MQ=60)
                                                 GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGG                                            <  SRR4302540.9556344/94‑1 (MQ=60)
                                                 GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTAGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGG                                            >  SRR4302540.2766652/1‑94 (MQ=60)
                                                  ATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGA                                           <  SRR4302540.2386600/94‑1 (MQ=60)
                                                  ATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGA                                           <  SRR4302540.2386601/94‑1 (MQ=60)
                                                  ATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGA                                           >  SRR4302540.10050965/1‑94 (MQ=60)
                                                   TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTCCCGCGATCGATATGGAT                                          >  SRR4302540.2861346/1‑94 (MQ=60)
                                                   TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAT                                          >  SRR4302540.1009660/1‑94 (MQ=60)
                                                   TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAT                                          <  SRR4302540.1783817/94‑1 (MQ=60)
                                                     GTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAA                                        >  SRR4302540.3397499/1‑94 (MQ=60)
                                                     GTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAA                                        >  SRR4302540.4462359/1‑94 (MQ=60)
                                                     GTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGGACACAAATTTATTTTTACCGCGATCGATATG‑‑‑AAAAA                                     >  SRR4302540.8747489/1‑94 (MQ=60)
                                                      TATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACGGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAA                                       >  SRR4302540.6652106/1‑94 (MQ=60)
                                                      TATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGGTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAA                                       >  SRR4302540.7172073/1‑94 (MQ=60)
                                                        TTATCAGTTGGGGCCAGCAATCCATCGGCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     <  SRR4302540.2801018/94‑1 (MQ=60)
                                                        TTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     >  SRR4302540.2742711/1‑94 (MQ=60)
                                                        TTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     >  SRR4302540.2224584/1‑94 (MQ=60)
                                                        TTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     >  SRR4302540.3824094/1‑94 (MQ=60)
                                                         TATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAC                                    <  SRR4302540.6817952/94‑1 (MQ=60)
                                                         TATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAC                                    <  SRR4302540.6112918/94‑1 (MQ=60)
                                                         TATCAGTTGGGGCCAGCAATCCATCGAATTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAC                                    <  SRR4302540.6817951/94‑1 (MQ=60)
                                                          ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATACAAACA                                   >  SRR4302540.1720658/1‑94 (MQ=60)
                                                          ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   <  SRR4302540.8323753/94‑1 (MQ=60)
                                                          ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   <  SRR4302540.4607755/94‑1 (MQ=60)
                                                          ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   <  SRR4302540.1381976/94‑1 (MQ=60)
                                                          ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   >  SRR4302540.8754965/1‑94 (MQ=60)
                                                          ATCACTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGTCACGACCGGCACGTACTCAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   <  SRR4302540.1381975/94‑1 (MQ=60)
                                                           TCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCG                                  >  SRR4302540.198985/1‑94 (MQ=60)
                                                            CAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGC                                 <  SRR4302540.5512329/94‑1 (MQ=60)
                                                             AGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCG                                >  SRR4302540.6134865/1‑94 (MQ=60)
                                                              GTTGGGGCCAGCAATCCATCGACTTGTGGTTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGT                               <  SRR4302540.2611304/94‑1 (MQ=60)
                                                                TGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCT                             <  SRR4302540.1298620/94‑1 (MQ=60)
                                                                TGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCT                             <  SRR4302540.7920945/94‑1 (MQ=60)
                                                                  GGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTT                           <  SRR4302540.782695/94‑1 (MQ=60)
                                                                   GGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTG                          <  SRR4302540.8793930/94‑1 (MQ=60)
                                                                    GCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGC                         <  SRR4302540.5654548/94‑1 (MQ=60)
                                                                     CCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCT                        <  SRR4302540.7854985/94‑1 (MQ=60)
                                                                      CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302540.6426699/1‑94 (MQ=60)
                                                                      CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302540.5611531/1‑94 (MQ=60)
                                                                      CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302540.7861738/1‑94 (MQ=60)
                                                                      CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302540.4599157/1‑94 (MQ=60)
                                                                       AGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCA                      >  SRR4302540.8281924/1‑94 (MQ=60)
                                                                       AGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCA                      >  SRR4302540.4310070/1‑94 (MQ=60)
                                                                       AGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCA                      >  SRR4302540.7017113/1‑94 (MQ=60)
                                                                          AATCCATCGACTTGTGGGTTGGCTACGACCGTCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   >  SRR4302540.2969659/1‑94 (MQ=60)
                                                                          AATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   >  SRR4302540.2893214/1‑94 (MQ=60)
                                                                          AATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   >  SRR4302540.2480759/1‑94 (MQ=60)
                                                                          AATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   >  SRR4302540.2129767/1‑94 (MQ=60)
                                                                           ATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGG                  >  SRR4302540.5926564/1‑94 (MQ=60)
                                                                            TCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGT                 >  SRR4302540.5190634/1‑94 (MQ=60)
                                                                             CCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTT                >  SRR4302540.321718/1‑94 (MQ=60)
                                                                               ATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAC              >  SRR4302540.1618166/1‑94 (MQ=60)
                                                                               ATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAC              <  SRR4302540.1615133/94‑1 (MQ=60)
                                                                                  GACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTC           >  SRR4302540.4854111/1‑94 (MQ=60)
                                                                                   GCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302540.770206/94‑1 (MQ=60)
                                                                                   GCTTGTGGGTTGGCTACGACCGCCAAGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302540.259817/94‑1 (MQ=60)
                                                                                   ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          >  SRR4302540.3320942/1‑94 (MQ=60)
                                                                                   ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          >  SRR4302540.9851747/1‑94 (MQ=60)
                                                                                   ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302540.1355437/94‑1 (MQ=60)
                                                                                   ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302540.2617056/94‑1 (MQ=60)
                                                                                   ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302540.259818/94‑1 (MQ=60)
                                                                                    CTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAG         >  SRR4302540.9345387/1‑94 (MQ=60)
                                                                                     TTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGT        >  SRR4302540.7770410/1‑94 (MQ=60)
                                                                                     TTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGT        >  SRR4302540.7770411/1‑94 (MQ=60)
                                                                                      TGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC       <  SRR4302540.9296279/94‑1 (MQ=60)
                                                                                       GTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG      <  SRR4302540.8769884/94‑1 (MQ=60)
                                                                                       GTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG      >  SRR4302540.1017887/1‑94 (MQ=60)
                                                                                           GTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  <  SRR4302540.1593993/94‑1 (MQ=60)
                                                                                           |                                                                                             
CGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  >  NC_000913/974273‑974457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑