Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 974,364 A→G I275V (ATT→GTT)  mukF → MukF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913974,3640AG99.5% 670.0 / NA 210I275V (ATT→GTT) mukFMukF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (114/94);  minor base C (0/1);  total (114/96)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC  >  NC_000913/974274‑974452
                                                                                          |                                                                                        
gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                        >  2:4589166/1‑93 (MQ=255)
 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                       >  1:5043453/1‑93 (MQ=255)
 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                       >  2:2573469/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      >  1:4452571/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      >  1:1866519/1‑93 (MQ=255)
  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      <  2:3766921/93‑1 (MQ=255)
   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                     <  1:4169779/93‑1 (MQ=255)
    tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCt                                                                                    >  1:819592/1‑93 (MQ=255)
    tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCt                                                                                    >  1:3400012/1‑93 (MQ=255)
       aTTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGGTGGCTACg                                                                                 >  2:1791170/1‑93 (MQ=255)
        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCACTCCATCGACTTGTGGGTTGGCTACGa                                                                                <  1:5456772/93‑1 (MQ=255)
        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACgc                                                                                >  1:3867380/1‑92 (MQ=255)
        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                <  1:5837302/93‑1 (MQ=255)
          tcgtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAcc                                                                              <  1:3260489/93‑1 (MQ=255)
            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                            >  2:5486974/1‑93 (MQ=255)
            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                            >  1:3493194/1‑93 (MQ=255)
             tcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGcc                                                                           >  2:172391/1‑93 (MQ=255)
             tcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGcc                                                                           >  2:1378594/1‑93 (MQ=255)
              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                          <  2:1023354/93‑1 (MQ=255)
              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                          >  1:3580819/1‑93 (MQ=255)
               gATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAc                                                                         >  1:5971732/1‑93 (MQ=255)
                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  2:4297206/1‑93 (MQ=255)
                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  2:1208199/1‑93 (MQ=255)
                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  1:1609279/1‑93 (MQ=255)
                  cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTa                                                                      <  1:209766/93‑1 (MQ=255)
                  cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTa                                                                      <  1:3111764/93‑1 (MQ=255)
                   gTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTac                                                                     >  2:5145220/1‑93 (MQ=255)
                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                    >  1:3426018/1‑93 (MQ=255)
                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                  >  2:5337328/1‑93 (MQ=255)
                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                              <  2:2749040/93‑1 (MQ=255)
                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                             >  2:6001082/1‑93 (MQ=255)
                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                             >  2:498121/1‑93 (MQ=255)
                            tCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTa                                                            >  1:2846156/1‑93 (MQ=255)
                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                         <  2:597072/93‑1 (MQ=255)
                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                         <  1:4974057/93‑1 (MQ=255)
                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        <  1:1667054/93‑1 (MQ=255)
                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        >  1:2951998/1‑93 (MQ=255)
                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        >  2:2960889/1‑93 (MQ=255)
                                 cTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                       >  1:3176431/1‑93 (MQ=255)
                                 cTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                       <  2:3693922/93‑1 (MQ=255)
                                  tggagagCAAGCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTGGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGtg                                                      <  1:1924131/90‑2 (MQ=255)
                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                      <  1:4127249/93‑1 (MQ=255)
                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                     >  2:2099070/1‑93 (MQ=255)
                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                   <  2:2459323/93‑1 (MQ=255)
                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                   >  2:6217674/1‑93 (MQ=255)
                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                  <  1:3819451/93‑1 (MQ=255)
                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTCGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                  <  1:3384558/93‑1 (MQ=255)
                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                               >  1:4731146/1‑93 (MQ=255)
                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                               <  1:3922901/93‑1 (MQ=255)
                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                              >  1:1230617/1‑93 (MQ=255)
                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                              <  2:5526582/93‑1 (MQ=255)
                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                            >  1:5769130/1‑93 (MQ=255)
                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                            >  2:4263340/1‑93 (MQ=255)
                                             cTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcgat                                           >  1:1374797/1‑93 (MQ=255)
                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                          <  1:1191553/93‑1 (MQ=255)
                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                       >  2:5375972/1‑93 (MQ=255)
                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                      >  1:1004643/1‑93 (MQ=255)
                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     >  1:5383670/1‑93 (MQ=255)
                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     >  2:4212128/1‑93 (MQ=255)
                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     <  2:287134/93‑1 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  1:5340511/1‑93 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  <  1:1308715/93‑1 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  2:5022914/1‑93 (MQ=255)
                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  2:2388656/1‑93 (MQ=255)
                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                 >  1:4043998/1‑93 (MQ=255)
                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                <  2:5126263/93‑1 (MQ=255)
                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                              >  1:4280582/1‑93 (MQ=255)
                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           >  2:1556065/1‑93 (MQ=255)
                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           <  1:6104249/93‑1 (MQ=255)
                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGCTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           <  2:2280448/93‑1 (MQ=255)
                                                               tGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTc                         >  1:4544672/1‑93 (MQ=255)
                                                                ggggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                        <  1:2298168/93‑1 (MQ=255)
                                                                 gggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCtt                       <  2:1258687/93‑1 (MQ=255)
                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                     <  2:2696539/93‑1 (MQ=255)
                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                     <  1:3069679/93‑1 (MQ=255)
                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCTTCTTTGc                    <  1:5493554/93‑1 (MQ=255)
                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGc                    <  1:3229787/93‑1 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  <  1:4080739/93‑1 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  1:5273951/1‑93 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  <  2:5225583/93‑1 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  2:4408335/1‑93 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  1:6213543/1‑93 (MQ=255)
                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  2:4084058/1‑93 (MQ=255)
                                                                       ttaaTCCATCGACTTGTGGGATGGCTACTACCGCCACGTACTCAAATTTATTTGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTcg                 >  2:6113703/3‑92 (MQ=255)
                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                 <  1:2475271/93‑1 (MQ=255)
                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc               >  1:148490/1‑93 (MQ=255)
                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt            >  2:19371/1‑93 (MQ=255)
                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt           >  1:2175017/1‑93 (MQ=255)
                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt           >  2:1647810/1‑93 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          >  2:3945156/1‑93 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          <  2:581186/93‑1 (MQ=255)
                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          <  1:5939862/93‑1 (MQ=255)
                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt       <  2:3653222/93‑1 (MQ=255)
                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt       <  1:2749411/93‑1 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc      <  1:727616/93‑1 (MQ=255)
                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc      <  1:2922893/93‑1 (MQ=255)
                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa     >  2:3060504/1‑93 (MQ=255)
                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt   <  1:5151751/93‑1 (MQ=255)
                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc  <  1:2022692/93‑1 (MQ=255)
                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc  <  1:4641722/93‑1 (MQ=255)
                                                                                          |                                                                                        
GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC  >  NC_000913/974274‑974452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  >  NC_000913/974271‑974457
                                                                                             |                                                                                             
GACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGG                                                                                               >  SRR4302533.3370132/1‑94 (MQ=60)
 ACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGT                                                                                              <  SRR4302533.4812240/94‑1 (MQ=60)
 ACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGT                                                                                              >  SRR4302533.1861222/1‑94 (MQ=60)
   GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            <  SRR4302533.4577170/94‑1 (MQ=60)
   GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTG                                                                                            >  SRR4302533.5073976/1‑94 (MQ=60)
    ATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGG                                                                                           >  SRR4302533.1877903/1‑94 (MQ=60)
    ATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGG                                                                                           >  SRR4302533.4479491/1‑94 (MQ=60)
      CTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCT                                                                                         >  SRR4302533.824396/1‑94 (MQ=60)
      CTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCT                                                                                         <  SRR4302533.4195046/94‑1 (MQ=60)
      CTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCT                                                                                         >  SRR4302533.3421006/1‑94 (MQ=60)
       TGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTA                                                                                        >  SRR4302533.3865664/1‑94 (MQ=60)
        GCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTAC                                                                                       <  SRR4302533.3571546/94‑1 (MQ=60)
         CATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACG                                                                                      >  SRR4302533.4427641/1‑94 (MQ=60)
          ATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGC                                                                                     >  SRR4302533.3890986/1‑94 (MQ=60)
           TTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCACTCCATCGACTTGTGGGTTGGCTACGAC                                                                                    <  SRR4302533.5489648/94‑1 (MQ=60)
           TTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAC                                                                                    <  SRR4302533.5872597/94‑1 (MQ=60)
            TTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACC                                                                                   <  SRR4302533.3168277/94‑1 (MQ=60)
             TCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCG                                                                                  <  SRR4302533.3280602/94‑1 (MQ=60)
              CGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGC                                                                                 >  SRR4302533.3514737/1‑94 (MQ=60)
               GTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCC                                                                                <  SRR4302533.2803142/94‑1 (MQ=60)
               GTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCC                                                                                >  SRR4302533.166454/1‑94 (MQ=60)
               GTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCC                                                                                <  SRR4302533.2965428/94‑1 (MQ=60)
               GTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCC                                                                                <  SRR4302533.2056686/94‑1 (MQ=60)
                TCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCA                                                                               >  SRR4302533.3602881/1‑94 (MQ=60)
                 CGATCGTCTGGTGTTCGATCTGCAGAGTAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAC                                                                              <  SRR4302533.6269685/94‑1 (MQ=60)
                 CGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAC                                                                              >  SRR4302533.6007880/1‑94 (MQ=60)
                  GATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGATCGCCACG                                                                             >  SRR4302533.158579/1‑94 (MQ=60)
                  GATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACG                                                                             >  SRR4302533.1619039/1‑94 (MQ=60)
                     CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          <  SRR4302533.211091/94‑1 (MQ=60)
                     CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          <  SRR4302533.3130898/94‑1 (MQ=60)
                     CGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAC                                                                          <  SRR4302533.2891762/94‑1 (MQ=60)
                      GTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACA                                                                         >  SRR4302533.3447181/1‑94 (MQ=60)
                      GTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACA                                                                         >  SRR4302533.370663/1‑94 (MQ=60)
                          GGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAA                                                                     >  SRR4302533.5854955/1‑94 (MQ=60)
                             GTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGCACACAAATTT                                                                  >  SRR4302533.5461652/1‑94 (MQ=60)
                              TTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTA                                                                 >  SRR4302533.323211/1‑94 (MQ=60)
                              TTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTA                                                                 >  SRR4302533.4259879/1‑94 (MQ=60)
                              TTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTA                                                                 >  SRR4302533.2863673/1‑94 (MQ=60)
                                 GATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTC                                                              <  SRR4302533.6095081/94‑1 (MQ=60)
                                  ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302533.2970050/1‑94 (MQ=60)
                                  ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             >  SRR4302533.4781706/1‑94 (MQ=60)
                                  ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             <  SRR4302533.3903841/94‑1 (MQ=60)
                                  ATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCG                                                             <  SRR4302533.5004217/94‑1 (MQ=60)
                                   TCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGT                                                            <  SRR4302533.3412672/94‑1 (MQ=60)
                                   TCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGT                                                            <  SRR4302533.1677125/94‑1 (MQ=60)
                                   TCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGT                                                            >  SRR4302533.3195970/1‑94 (MQ=60)
                                   TCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGT                                                            >  SRR4302533.5025895/1‑94 (MQ=60)
                                     TGGAGAGCAAGCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTGGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTGC                                                          <  SRR4302533.1935869/94‑1 (MQ=60)
                                     TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          <  SRR4302533.4152305/94‑1 (MQ=60)
                                     TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAC                                                          <  SRR4302533.4152304/94‑1 (MQ=60)
                                     TGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATACATCGACTTGTGGGTTGGCTACCACCGCCACGTACACAAATTTATgctgtc                                                          >  SRR4302533.2302730/1‑88 (MQ=60)
                                       CAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCG                                                        <  SRR4302533.4047745/94‑1 (MQ=60)
                                         GAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCG                                                      >  SRR4302533.2535680/1‑94 (MQ=60)
                                         GAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCG                                                      <  SRR4302533.3842807/94‑1 (MQ=60)
                                         GAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTCGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCG                                                      <  SRR4302533.3405454/94‑1 (MQ=60)
                                           GCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGAT                                                    >  SRR4302533.4759830/1‑94 (MQ=60)
                                            CAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATC                                                   <  SRR4302533.6201540/94‑1 (MQ=60)
                                            CAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATC                                                   <  SRR4302533.3946853/94‑1 (MQ=60)
                                            CAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATC                                                   >  SRR4302533.1237987/1‑94 (MQ=60)
                                              AACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGA                                                 <  SRR4302533.5091047/94‑1 (MQ=60)
                                              AACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGA                                                 >  SRR4302533.5803959/1‑94 (MQ=60)
                                               ACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGAT                                                >  SRR4302533.1383069/1‑94 (MQ=60)
                                                CTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACCTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATA                                               >  SRR4302533.3434048/1‑94 (MQ=60)
                                                 TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              >  SRR4302533.1926737/1‑94 (MQ=60)
                                                 TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              <  SRR4302533.1198711/94‑1 (MQ=60)
                                                 TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              >  SRR4302533.413132/1‑94 (MQ=60)
                                                 TCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAT                                              >  SRR4302533.3268526/1‑94 (MQ=60)
                                                  CGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATG                                             <  SRR4302533.2855066/94‑1 (MQ=60)
                                                   GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGG                                            >  SRR4302533.3454747/1‑94 (MQ=60)
                                                   GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGG                                            >  SRR4302533.5953822/1‑94 (MQ=60)
                                                    ATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGA                                           >  SRR4302533.1010520/1‑94 (MQ=60)
                                                    ATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGA                                           >  SRR4302533.3893236/1‑94 (MQ=60)
                                                     TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAT                                          <  SRR4302533.1965406/94‑1 (MQ=60)
                                                     TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAT                                          >  SRR4302533.5416014/1‑94 (MQ=60)
                                                     TCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAT                                          <  SRR4302533.5388802/94‑1 (MQ=60)
                                                        TATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAA                                       >  SRR4302533.3312945/1‑94 (MQ=60)
                                                        TATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAA                                       >  SRR4302533.5372536/1‑94 (MQ=60)
                                                         ATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAA                                      >  SRR4302533.4510361/1‑94 (MQ=60)
                                                         ATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAA                                      >  SRR4302533.1474223/1‑94 (MQ=60)
                                                         ATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAA                                      <  SRR4302533.1316571/94‑1 (MQ=60)
                                                         ATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAA                                      <  SRR4302533.5461284/94‑1 (MQ=60)
                                                         ATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAA                                      >  SRR4302533.4068612/1‑94 (MQ=60)
                                                          TTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     <  SRR4302533.790746/94‑1 (MQ=60)
                                                          TTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAA                                     >  SRR4302533.1843164/1‑94 (MQ=60)
                                                            ATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACC                                   >  SRR4302533.4306316/1‑94 (MQ=60)
                                                              CAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGC                                 <  SRR4302533.132969/94‑1 (MQ=60)
                                                               ATTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCG                                >  SRR4302533.2256926/1‑94 (MQ=60)
                                                               AGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCG                                <  SRR4302533.331819/94‑1 (MQ=60)
                                                               AGTGGGGGCCAGCAATCCATCGACTTGGGGTTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCTATCGATATGGATAAAAACCGCG                                <  SRR4302533.21641/94‑1 (MQ=60)
                                                                GTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGT                               <  SRR4302533.6141093/94‑1 (MQ=60)
                                                                 TTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTC                              >  SRR4302533.4572195/1‑94 (MQ=60)
                                                                  TGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCT                             <  SRR4302533.5206411/94‑1 (MQ=60)
                                                                   GGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTT                            <  SRR4302533.2312422/94‑1 (MQ=60)
                                                                      GCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGC                         <  SRR4302533.3088524/94‑1 (MQ=60)
                                                                       GCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACAGCGATCGATATGGATAAAAACCGCGTCTTTGCT                        <  SRR4302533.4092831/94‑1 (MQ=60)
                                                                       CCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCTTCTTTGCT                        <  SRR4302533.5526692/94‑1 (MQ=60)
                                                                       CCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCT                        >  SRR4302533.5551524/1‑94 (MQ=60)
                                                                       CCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCT                        <  SRR4302533.3249698/94‑1 (MQ=60)
                                                                        CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302533.6250802/1‑94 (MQ=60)
                                                                        CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302533.5305572/1‑94 (MQ=60)
                                                                        CAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAACGCGATCGATATGGATAAAAACCGCGTCTTTGCTC                       >  SRR4302533.621600/1‑94 (MQ=60)
                                                                         AGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCA                      <  SRR4302533.4105583/94‑1 (MQ=60)
                                                                         AGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCA                      >  SRR4302533.1267151/1‑94 (MQ=60)
                                                                          GCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAG                     <  SRR4302533.1796732/94‑1 (MQ=60)
                                                                          GCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAG                     <  SRR4302533.2490537/94‑1 (MQ=60)
                                                                           CAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGC                    >  SRR4302533.149439/1‑94 (MQ=60)
                                                                           CAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGC                    >  SRR4302533.3110932/1‑94 (MQ=60)
                                                                            AATCCATCGACTTGTGGGTTGGCTACGACCGGCACGTACACAAATTTATTCGGACCGCGATCGATATGGATAAAAACCGCGTCTTGGCTCAGCG                   >  SRR4302533.896945/1‑94 (MQ=60)
                                                                            AATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   <  SRR4302533.1192956/94‑1 (MQ=60)
                                                                            AATCCACCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCG                   <  SRR4302533.6007275/94‑1 (MQ=60)
                                                                               CCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTT                >  SRR4302533.2188420/1‑94 (MQ=60)
                                                                                CATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTA               >  SRR4302533.4616614/1‑94 (MQ=60)
                                                                                 ATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAC              <  SRR4302533.5975776/94‑1 (MQ=60)
                                                                                  TCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACG             <  SRR4302533.5522079/94‑1 (MQ=60)
                                                                                  TCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACG             <  SRR4302533.2457282/94‑1 (MQ=60)
                                                                                  ACGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACG             <  SRR4302533.1213739/94‑1 (MQ=60)
                                                                                   CGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGT            >  SRR4302533.5325932/1‑94 (MQ=60)
                                                                                   CGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGT            <  SRR4302533.474030/94‑1 (MQ=60)
                                                                                    GACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTC           <  SRR4302533.2997470/94‑1 (MQ=60)
                                                                                    GACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTC           <  SRR4302533.2766267/94‑1 (MQ=60)
                                                                                     ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302533.2940791/94‑1 (MQ=60)
                                                                                     ACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCA          <  SRR4302533.731914/94‑1 (MQ=60)
                                                                                        TGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC       <  SRR4302533.5182870/94‑1 (MQ=60)
                                                                                         GTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG      <  SRR4302533.4669845/94‑1 (MQ=60)
                                                                                         GTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG      <  SRR4302533.2035149/94‑1 (MQ=60)
                                                                                            GGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTA   <  SRR4302533.5182316/94‑1 (MQ=60)
                                                                                             GTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  >  SRR4302533.4324629/1‑94 (MQ=60)
                                                                                             |                                                                                             
GACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCGGTAC  >  NC_000913/974271‑974457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑