Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,021,376	A→G	D580D (GAT→GAC)	b0960 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,021,376	0	A	G	100.0%	739.0 / NA	233	D580D (GAT→GAC)	b0960	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (89/144); total (89/144)

Only 100 of 239 total aligned reads displayed.

AAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCC  >  NC_000913/1021286‑1021463

 aaCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTcg                                                                                      >  2:7792421/1‑93 (MQ=255)

  aCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCTACGCCAGCTCAGCATCACGGTTGTGTGCGTcgc                                                                                     >  1:8192711/1‑93 (MQ=255)

  aCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTcgc                                                                                     <  1:7692880/93‑1 (MQ=255)

   cgcgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGCTGTGTGCGTcgcg                                                                                    >  2:1199700/1‑93 (MQ=255)

     cgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggc                                                                                  >  2:23916/1‑93 (MQ=255)

      ggCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggcg                                                                                 <  2:5776326/93‑1 (MQ=255)

      ggCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggcg                                                                                 <  1:3573657/93‑1 (MQ=255)

       gCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGcggcgg                                                                                <  2:16509/93‑1 (MQ=255)

       gCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGcggcgg                                                                                >  2:4148658/1‑93 (MQ=255)

       gCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGcggcgg                                                                                >  1:8899914/1‑93 (MQ=255)

        cTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCggg                                                                               >  2:8309368/1‑93 (MQ=255)

          tCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCa                                                                             >  1:6798652/1‑93 (MQ=255)

           cgcgAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCaa                                                                            <  1:8809482/93‑1 (MQ=255)

             cgAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGCCAata                                                                          <  1:6126757/93‑1 (MQ=255)

              gAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatag                                                                         >  2:6635231/1‑92 (MQ=255)

              gAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatac                                                                         >  1:1036191/1‑93 (MQ=255)

               aaTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacg                                                                        >  1:3977889/1‑93 (MQ=255)

               aaTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAataag                                                                        >  2:2629179/1‑91 (MQ=255)

                aTTTGCGGGGTAACGTCCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacga                                                                       <  2:1521869/93‑1 (MQ=255)

                 tttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgat                                                                      <  1:9638712/93‑1 (MQ=255)

                 tttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgat                                                                      <  1:1570581/93‑1 (MQ=255)

                 tttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgat                                                                      <  1:5742272/93‑1 (MQ=255)

                  ttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgata                                                                     <  2:4924479/93‑1 (MQ=255)

                  ttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgata                                                                     <  1:6219927/93‑1 (MQ=255)

                  ttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgata                                                                     <  1:5051826/93‑1 (MQ=255)

                      ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGcc                                                                 <  2:2825816/93‑1 (MQ=255)

                      ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGcc                                                                 >  1:2464964/1‑93 (MQ=255)

                       gggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCa                                                                <  1:5836982/93‑1 (MQ=255)

                       gggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCa                                                                <  2:5362474/93‑1 (MQ=255)

                         gTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGa                                                              >  1:7458621/1‑93 (MQ=255)

                             cGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGt                                                          >  2:5913952/1‑93 (MQ=255)

                              gTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGtt                                                         >  2:56651/1‑93 (MQ=255)

                               ttCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTa                                                        >  2:275478/1‑93 (MQ=255)

                               ttCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTa                                                        <  1:9471164/93‑1 (MQ=255)

                                 cGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATc                                                      >  1:375466/1‑93 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  2:5243265/93‑1 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  2:54976/93‑1 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  1:5949108/93‑1 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  1:4148111/93‑1 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  1:4370839/93‑1 (MQ=255)

                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                     <  2:3217515/93‑1 (MQ=255)

                                   gCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAc                                                    <  2:1878328/93‑1 (MQ=255)

                                   gCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAc                                                    >  1:3015223/1‑93 (MQ=255)

                                      cGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGcc                                                 <  2:8372120/93‑1 (MQ=255)

                                        cTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGcccc                                               <  1:1662493/93‑1 (MQ=255)

                                           gACATATTTGATACCACCGACGCCAGCTCAGCATCCCGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGa                                            <  1:7366494/93‑1 (MQ=255)

                                           gACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGa                                            >  1:6430441/1‑93 (MQ=255)

                                           gACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGa                                            >  1:2663327/1‑93 (MQ=255)

                                            aCATATTTGATGCCACCGACGCCCGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                           <  1:9359454/93‑1 (MQ=255)

                                            aCATATTTGATCCCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                           <  2:7581239/93‑1 (MQ=255)

                                            aCATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                           <  1:8270606/93‑1 (MQ=255)

                                            aCATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                           <  2:6892431/93‑1 (MQ=255)

                                             cATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATg                                          <  2:1045725/93‑1 (MQ=255)

                                              atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATgg                                         <  1:2782212/93‑1 (MQ=255)

                                                atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTa                                       <  2:6767935/93‑1 (MQ=255)

                                                atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTa                                       <  1:107102/93‑1 (MQ=255)

                                                 tttGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAt                                      <  2:4441016/93‑1 (MQ=255)

                                                 tttAATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACCCCCCTGCTggaac                                      >  1:1395935/1‑90 (MQ=255)

                                                  ttGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAtt                                     >  1:2870840/1‑93 (MQ=255)

                                                    gATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGc                                   <  2:10016922/93‑1 (MQ=255)

                                                      tACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTc                                 >  2:949499/1‑93 (MQ=255)

                                                        ccaccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCa                               >  1:5420087/1‑93 (MQ=255)

                                                          cccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGt                             <  1:6009658/92‑1 (MQ=255)

                                                           ccGACGGCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                            >  2:7204696/1‑93 (MQ=255)

                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                            <  1:6706011/93‑1 (MQ=255)

                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                            <  2:597551/93‑1 (MQ=255)

                                                            cGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAt                           <  1:9156760/93‑1 (MQ=255)

                                                            cGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAt                           >  1:3222314/1‑93 (MQ=255)

                                                               cGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGc                        <  1:1786836/93‑1 (MQ=255)

                                                               cGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGACAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGc                        >  2:471237/1‑93 (MQ=255)

                                                                gCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCa                       >  2:3039262/1‑93 (MQ=255)

                                                                gCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCa                       <  2:193825/93‑1 (MQ=255)

                                                                gCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCa                       <  2:6683349/93‑1 (MQ=255)

                                                                 ccAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCAt                      <  1:1964232/93‑1 (MQ=255)

                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                     >  1:1105959/1‑93 (MQ=255)

                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                     <  2:7449791/93‑1 (MQ=255)

                                                                     cTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgag                  >  2:2650218/1‑93 (MQ=255)

                                                                     cTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgag                  <  1:6892527/93‑1 (MQ=255)

                                                                      tCAGCATCGCGGGTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                 <  1:5289104/93‑1 (MQ=255)

                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                 <  1:2227400/93‑1 (MQ=255)

                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                 >  1:3195152/1‑93 (MQ=255)

                                                                       cAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAt                <  2:1128137/93‑1 (MQ=255)

                                                                       cAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAt                >  2:7452235/1‑93 (MQ=255)

                                                                        aGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATa               <  1:4216725/93‑1 (MQ=255)

                                                                           aTCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCg            <  2:8517899/93‑1 (MQ=255)

                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATTGCATCGAGATACCGa           >  1:8965348/1‑93 (MQ=255)

                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa           >  1:3890671/1‑93 (MQ=255)

                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa           <  2:9191498/93‑1 (MQ=255)

                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa           >  2:4523350/1‑93 (MQ=255)

                                                                             cACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGAc          >  1:7189639/1‑93 (MQ=255)

                                                                              aCGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACa         <  2:3952135/93‑1 (MQ=255)

                                                                               cGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAg        <  1:6098540/93‑1 (MQ=255)

                                                                               cGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAg        <  2:4408967/93‑1 (MQ=255)

                                                                                ggTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGt       <  2:9773131/93‑1 (MQ=255)

                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt      >  1:4543030/1‑93 (MQ=255)

                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt      >  2:6307937/1‑93 (MQ=255)

                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt      <  1:8091894/93‑1 (MQ=255)

                                                                                    gtgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGc   >  1:4968174/1‑93 (MQ=255)

                                                                                     tgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGcc  >  1:7910614/1‑93 (MQ=255)

                                                                                     tgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGcc  <  2:3061696/93‑1 (MQ=255)

AAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCC  >  NC_000913/1021286‑1021463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  NC_000913/1021285‑1021469

GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTC                                                                                             >  SRR4302540.8083827/1‑94 (MQ=60)

GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTC                                                                                             >  SRR4302540.3370300/1‑94 (MQ=60)

  AACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCTACGCCAGCTCAGCATCACGGTTGTGTGCGTCGC                                                                                           >  SRR4302540.8287904/1‑94 (MQ=60)

   ACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCG                                                                                          <  SRR4302540.7780425/94‑1 (MQ=60)

   ACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCG                                                                                          <  SRR4302540.7780426/94‑1 (MQ=60)

       GGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGG                                                                                      >  SRR4302540.9002944/1‑94 (MQ=60)

       GGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGG                                                                                      <  SRR4302540.3612689/94‑1 (MQ=60)

        GCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGG                                                                                     <  SRR4302540.134651/94‑1 (MQ=60)

        GCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGG                                                                                     <  SRR4302540.2998368/94‑1 (MQ=60)

         CTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGC                                                                                    >  SRR4302540.4257231/1‑94 (MQ=60)

         CTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGC                                                                                    >  SRR4302540.6315612/1‑94 (MQ=60)

         CTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGC                                                                                    <  SRR4302540.2712913/94‑1 (MQ=60)

          TTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCA                                                                                   >  SRR4302540.6873858/1‑94 (MQ=60)

           TCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAA                                                                                  <  SRR4302540.1887483/94‑1 (MQ=60)

            CGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAAT                                                                                 <  SRR4302540.8911332/94‑1 (MQ=60)

             GCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATA                                                                                <  SRR4302540.8360502/94‑1 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               <  SRR4302540.44735/94‑1 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               <  SRR4302540.8735554/94‑1 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               >  SRR4302540.1046729/1‑94 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               <  SRR4302540.1108033/94‑1 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               >  SRR4302540.1046731/1‑94 (MQ=60)

              CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGCCAATAC                                                                               <  SRR4302540.6194995/94‑1 (MQ=60)

               GAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACG                                                                              >  SRR4302540.4020741/1‑94 (MQ=60)

                  TTTGCGGTGTAACGTTCGGCTCGCTGGACATATTTGATCCCACCGACGCCAGCTCAGCATCCCGGTTGTGTGCGTCGCGGCGGGCAATACGATA                                                                           <  SRR4302540.3248138/94‑1 (MQ=60)

                  TTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATA                                                                           <  SRR4302540.9752260/94‑1 (MQ=60)

                  TTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATA                                                                           <  SRR4302540.3568728/94‑1 (MQ=60)

                  TTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATA                                                                           <  SRR4302540.5805571/94‑1 (MQ=60)

                  TTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATA                                                                           <  SRR4302540.1587794/94‑1 (MQ=60)

                   TTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATAC                                                                          <  SRR4302540.5722141/94‑1 (MQ=60)

                   TTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATAC                                                                          <  SRR4302540.6289121/94‑1 (MQ=60)

                   TTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATAC                                                                          <  SRR4302540.5218183/94‑1 (MQ=60)

                   TTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATAC                                                                          <  SRR4302540.5106443/94‑1 (MQ=60)

                    TGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACG                                                                         <  SRR4302540.6283416/94‑1 (MQ=60)

                      CGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCC                                                                       >  SRR4302540.2491495/1‑94 (MQ=60)

                       GGGGTAACGTTCGGCTCGCTGGACATATTTGATACCCCCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCA                                                                      <  SRR4302540.6254304/94‑1 (MQ=60)

                       GGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCA                                                                      >  SRR4302540.7449349/1‑94 (MQ=60)

                        GGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAG                                                                     <  SRR4302540.7268878/94‑1 (MQ=60)

                        GGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAG                                                                     <  SRR4302540.5901520/94‑1 (MQ=60)

                         GGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGA                                                                    >  SRR4302540.7541781/1‑94 (MQ=60)

                               GTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTA                                                              <  SRR4302540.8023721/94‑1 (MQ=60)

                                TTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTAT                                                             <  SRR4302540.9583509/94‑1 (MQ=60)

                                 TCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATC                                                            >  SRR4302540.379355/1‑94 (MQ=60)

                                  CGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCA                                                           >  SRR4302540.5831986/1‑94 (MQ=60)

                                  CGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCA                                                           >  SRR4302540.9478808/1‑94 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302540.4418320/94‑1 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302540.4193019/94‑1 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302540.6014894/94‑1 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302540.4118010/94‑1 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          >  SRR4302540.3048375/1‑94 (MQ=60)

                                   GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGACGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          >  SRR4302540.4352140/1‑94 (MQ=60)

                                   GGCCCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302540.2650189/94‑1 (MQ=60)

                                     CTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGC                                                        >  SRR4302540.940663/1‑94 (MQ=60)

                                     CTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGC                                                        >  SRR4302540.975176/1‑94 (MQ=60)

                                      TCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCC                                                       >  SRR4302540.8970891/1‑94 (MQ=60)

                                       CGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCC                                                      <  SRR4302540.381383/94‑1 (MQ=60)

                                        GCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACACCAGACGGTTATCACGCCCC                                                     <  SRR4302540.4883425/94‑1 (MQ=60)

                                         CTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCT                                                    >  SRR4302540.176382/1‑94 (MQ=60)

                                         CTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCT                                                    <  SRR4302540.3226196/94‑1 (MQ=60)

                                         CTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCT                                                    <  SRR4302540.1680594/94‑1 (MQ=60)

                                           GGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGA                                                  >  SRR4302540.2692211/1‑94 (MQ=60)

                                           GGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGA                                                  >  SRR4302540.6502018/1‑94 (MQ=60)

                                            GACATATTTGATACCACCGACGCCAGCTCAGCATCCCGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAT                                                 <  SRR4302540.7448622/94‑1 (MQ=60)

                                            GACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATAACGCCCCTGAT                                                 >  SRR4302540.5411731/1‑94 (MQ=60)

                                             ACATATTTGATGCCACCGACGCCCGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATG                                                <  SRR4302540.9470916/94‑1 (MQ=60)

                                             ACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATG                                                <  SRR4302540.8366699/94‑1 (MQ=60)

                                              CATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGG                                               <  SRR4302540.10055670/94‑1 (MQ=60)

                                               ATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGT                                              <  SRR4302540.2819958/94‑1 (MQ=60)

                                               ATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGT                                              <  SRR4302540.2812267/94‑1 (MQ=60)

                                               ATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGT                                              >  SRR4302540.3778847/1‑94 (MQ=60)

                                                 ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            >  SRR4302540.4987355/1‑94 (MQ=60)

                                                 ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            <  SRR4302540.108251/94‑1 (MQ=60)

                                                 ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGCTATCACGCCCCTGATGGTAT                                            >  SRR4302540.9564126/1‑94 (MQ=60)

                                                 ATTTAATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACCCCCCTGctggaac                                            >  SRR4302540.1410905/1‑87 (MQ=60)

                                                  TTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATT                                           >  SRR4302540.2902029/1‑94 (MQ=60)

                                                   TTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTG                                          <  SRR4302540.3888148/94‑1 (MQ=60)

                                                   TTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTG                                          <  SRR4302540.5923882/94‑1 (MQ=60)

                                                     GATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCT                                        >  SRR4302540.6061618/1‑94 (MQ=60)

                                                        ACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCA                                     >  SRR4302540.5479684/1‑94 (MQ=60)

                                                        ACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCA                                     >  SRR4302540.2278708/1‑94 (MQ=60)

                                                          CACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGT                                   <  SRR4302540.732782/94‑1 (MQ=60)

                                                           CCCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTA                                  <  SRR4302540.6076296/94‑1 (MQ=60)

                                                           ACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTA                                  <  SRR4302540.6076295/94‑1 (MQ=60)

                                                            CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 <  SRR4302540.7161816/94‑1 (MQ=60)

                                                            CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 <  SRR4302540.580581/94‑1 (MQ=60)

                                                            CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 >  SRR4302540.3257334/1‑94 (MQ=60)

                                                            CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 <  SRR4302540.6780021/94‑1 (MQ=60)

                                                             CGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATG                                <  SRR4302540.9266682/94‑1 (MQ=60)

                                                                CGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCA                             <  SRR4302540.1806249/94‑1 (MQ=60)

                                                                CGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCA                             <  SRR4302540.1806248/94‑1 (MQ=60)

                                                                CGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGAA                             <  SRR4302540.6156622/94‑1 (MQ=60)

                                                                 GCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCAT                            <  SRR4302540.9051700/94‑1 (MQ=60)

                                                                  CCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATC                           >  SRR4302540.1117369/1‑94 (MQ=60)

                                                                  CCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATC                           <  SRR4302540.1985567/94‑1 (MQ=60)

                                                                   CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          >  SRR4302540.3944340/1‑94 (MQ=60)

                                                                   CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          >  SRR4302540.2014635/1‑94 (MQ=60)

                                                                      CTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGA                       >  SRR4302540.7753259/1‑94 (MQ=60)

                                                                      CTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGA                       <  SRR4302540.6968997/94‑1 (MQ=60)

                                                                      CTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGA                       >  SRR4302540.3229854/1‑94 (MQ=60)

                                                                      CTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGA                       <  SRR4302540.3022403/94‑1 (MQ=60)

                                                                       TCAGCATCGCGGGTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      <  SRR4302540.5346911/94‑1 (MQ=60)

                                                                       TCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      >  SRR4302540.8267145/1‑94 (MQ=60)

                                                                       TCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      <  SRR4302540.2251396/94‑1 (MQ=60)

                                                                       TCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      <  SRR4302540.2163085/94‑1 (MQ=60)

                                                                       TCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      <  SRR4302540.66936/94‑1 (MQ=60)

                                                                       TCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAT                      <  SRR4302540.66937/94‑1 (MQ=60)

                                                                        CAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATA                     <  SRR4302540.1593899/94‑1 (MQ=60)

                                                                        CAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATA                     <  SRR4302540.1413785/94‑1 (MQ=60)

                                                                         AGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAC                    <  SRR4302540.4237020/94‑1 (MQ=60)

                                                                         AGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAC                    <  SRR4302540.9742997/94‑1 (MQ=60)

                                                                         AGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAC                    <  SRR4302540.6834006/94‑1 (MQ=60)

                                                                         AGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAC                    <  SRR4302540.4262631/94‑1 (MQ=60)

                                                                           CATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCG                  <  SRR4302540.5206107/94‑1 (MQ=60)

                                                                            ATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATTGCATCGAGATACCGA                 >  SRR4302540.9071574/1‑94 (MQ=60)

                                                                            ATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGA                 >  SRR4302540.3932915/1‑94 (MQ=60)

                                                                            ATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGA                 <  SRR4302540.2730138/94‑1 (MQ=60)

                                                                             TCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGAC                >  SRR4302540.7269778/1‑94 (MQ=60)

                                                                              CACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACA               <  SRR4302540.6944002/94‑1 (MQ=60)

                                                                                CGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGT             <  SRR4302540.6166431/94‑1 (MQ=60)

                                                                                CGGGTGTGTGAGTCGCGGGGGGCAATACGATACGCCAGACGGGTATCACGCCCCTGAGGGTATGGCTCCAGTATGGCATCGAGATACCGACAGT             <  SRR4302540.6166432/94‑1 (MQ=60)

                                                                                 GGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTT            >  SRR4302540.7852498/1‑94 (MQ=60)

                                                                                 GGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTT            >  SRR4302540.4592193/1‑94 (MQ=60)

                                                                                 GGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTT            >  SRR4302540.502399/1‑94 (MQ=60)

                                                                                 GGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGGATTGCTCCAGTATGGCATCGAGATACCGACAGTT            >  SRR4302540.920159/1‑94 (MQ=60)

                                                                                  GTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTG           <  SRR4302540.8185822/94‑1 (MQ=60)

                                                                                  GTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGGTG           >  SRR4302540.6204659/1‑94 (MQ=60)

                                                                                    TGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGC         >  SRR4302540.5021922/1‑94 (MQ=60)

                                                                                     GTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCC        >  SRR4302540.8511062/1‑94 (MQ=60)

                                                                                     GTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCC        >  SRR4302540.8002852/1‑94 (MQ=60)

                                                                                      TGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCT       <  SRR4302540.1325296/94‑1 (MQ=60)

                                                                                        TGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCT     >  SRR4302540.8779503/1‑94 (MQ=60)

                                                                                           GTCGCGGCGGGCAATACGATTCGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  SRR4302540.9856924/1‑94 (MQ=60)

                                                                                           GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTTGCCTCTGTG  >  SRR4302540.7771689/1‑94 (MQ=60)

                                                                                           GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  <  SRR4302540.4307035/94‑1 (MQ=60)

                                                                                           GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  <  SRR4302540.4511390/94‑1 (MQ=60)

GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  NC_000913/1021285‑1021469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`