Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,021,376 A→G D580D (GAT→GAC)  b0960 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,021,3760AG100.0% 599.4 / NA 187D580D (GAT→GAC) b0960hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (84/103);  total (84/103)

Only 100 of 197 total aligned reads displayed.

AAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTC  >  NC_000913/1021286‑1021465
                                                                                          |                                                                                         
aaaCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTc                                                                                         >  1:5056781/1‑93 (MQ=255)
aaaCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTc                                                                                         <  1:4810410/93‑1 (MQ=255)
aaaCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTc                                                                                         <  2:4196256/93‑1 (MQ=255)
  aCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTcgc                                                                                       >  2:4208890/1‑93 (MQ=255)
  aCGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTcgc                                                                                       >  1:5253468/1‑93 (MQ=255)
    gcgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcgg                                                                                     >  1:5189536/1‑93 (MQ=255)
    gcgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcgg                                                                                     >  2:3397145/1‑93 (MQ=255)
    gcgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcgg                                                                                     >  2:564125/1‑93 (MQ=255)
     cgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggc                                                                                    >  2:6272802/1‑93 (MQ=255)
     cgGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggc                                                                                    <  2:3148653/93‑1 (MQ=255)
      ggCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCgcggcg                                                                                   >  2:5961576/1‑93 (MQ=255)
       gCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGcggcgg                                                                                  >  2:2869286/1‑93 (MQ=255)
       gCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGCTGTGTGCGTCGcggcgg                                                                                  >  2:2414089/1‑93 (MQ=255)
          tCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCa                                                                               <  2:4088592/93‑1 (MQ=255)
             cgAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAata                                                                            <  2:5338385/93‑1 (MQ=255)
             cgAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAata                                                                            >  1:6233240/1‑93 (MQ=255)
             cgAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAata                                                                            <  1:2209816/93‑1 (MQ=255)
                aTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacga                                                                         <  1:3545947/93‑1 (MQ=255)
                aTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacga                                                                         >  2:3923223/1‑93 (MQ=255)
                aTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacga                                                                         >  2:3385576/1‑93 (MQ=255)
                  ttGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgata                                                                       <  2:5508475/93‑1 (MQ=255)
                   tGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAatacgatac                                                                      >  2:3246506/1‑93 (MQ=255)
                     cGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGc                                                                    <  2:4968523/93‑1 (MQ=255)
                      ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGcc                                                                   <  1:1528413/93‑1 (MQ=255)
                      ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGcc                                                                   >  1:69895/1‑93 (MQ=255)
                      ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGcc                                                                   <  1:4441109/93‑1 (MQ=255)
                         gTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGa                                                                >  2:31900/1‑93 (MQ=255)
                          tAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGAc                                                               >  2:5303512/1‑93 (MQ=255)
                            aCGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACgg                                                             >  2:974128/1‑93 (MQ=255)
                             cGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGt                                                            >  2:187596/1‑93 (MQ=255)
                               ttCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTa                                                          >  2:5478075/1‑93 (MQ=255)
                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                       <  1:2761265/93‑1 (MQ=255)
                                  ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCa                                                       <  1:1852548/93‑1 (MQ=255)
                                   gCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAc                                                      >  1:110100/1‑93 (MQ=255)
                                   gCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGGTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATTAc                                                      >  1:4293810/1‑93 (MQ=255)
                                    cTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACg                                                     >  1:3056018/1‑93 (MQ=255)
                                     tCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGc                                                    <  2:2591549/93‑1 (MQ=255)
                                     tCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGc                                                    >  2:6193434/1‑93 (MQ=255)
                                     tCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGc                                                    >  2:149292/1‑93 (MQ=255)
                                     tCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGc                                                    >  1:997168/1‑93 (MQ=255)
                                      cGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGcc                                                   <  1:5142226/93‑1 (MQ=255)
                                      cGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGcc                                                   >  2:4587152/1‑93 (MQ=255)
                                        cTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGcccc                                                 >  1:4992457/1‑93 (MQ=255)
                                         tGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCt                                                >  2:1033379/1‑93 (MQ=255)
                                            aCATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                             <  1:5960967/93‑1 (MQ=255)
                                            aCATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAt                                             <  1:5415600/93‑1 (MQ=255)
                                              ctatTTAATACCACCGACGCCAGCTCAGCATCGCGGTTGTGTGCGTCGCGGCGGCCAATACGATCCGCCAGACGGTTATCACGCCCCTGATgg                                           <  2:3283309/92‑1 (MQ=255)
                                               tatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGt                                          >  2:4837591/1‑93 (MQ=255)
                                               tatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGt                                          >  1:4067563/1‑93 (MQ=255)
                                                atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTa                                         <  1:2071233/93‑1 (MQ=255)
                                                atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTCCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTa                                         >  2:5131653/1‑93 (MQ=255)
                                                 tttGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAt                                        >  1:5664700/1‑93 (MQ=255)
                                                    gATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGc                                     >  2:4638474/1‑93 (MQ=255)
                                                    gATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGc                                     >  1:1650274/1‑93 (MQ=255)
                                                        ccaccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCa                                 <  2:2638027/93‑1 (MQ=255)
                                                         caccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAg                                <  2:680121/93‑1 (MQ=255)
                                                          accGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGt                               >  2:1559159/1‑93 (MQ=255)
                                                          accGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGt                               >  1:2249233/1‑93 (MQ=255)
                                                          accGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGt                               <  2:869581/93‑1 (MQ=255)
                                                          accGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGGTATCACGCCCCTGATGGTATTGCTCCAGt                               >  1:3158884/1‑93 (MQ=255)
                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                              <  2:2210799/93‑1 (MQ=255)
                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                              <  2:4999723/93‑1 (MQ=255)
                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                              <  1:1722124/93‑1 (MQ=255)
                                                           ccGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTa                              >  1:3818915/1‑93 (MQ=255)
                                                            cGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAt                             <  2:3479436/93‑1 (MQ=255)
                                                               cGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGc                          >  2:3700525/1‑93 (MQ=255)
                                                               cGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGc                          <  1:5715366/93‑1 (MQ=255)
                                                                gCCAGCTCAGCATCACGGTTGTGCGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCa                         >  2:4263558/1‑93 (MQ=255)
                                                                 ccAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCAt                        <  2:1897055/93‑1 (MQ=255)
                                                                 ccAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCAt                        >  1:2393942/1‑93 (MQ=255)
                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                       <  2:904581/93‑1 (MQ=255)
                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                       >  1:3955105/1‑93 (MQ=255)
                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                       <  1:3328723/93‑1 (MQ=255)
                                                                  cAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATc                       >  2:2474443/1‑93 (MQ=255)
                                                                   aGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCg                      >  2:4434129/1‑93 (MQ=255)
                                                                   aGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCg                      >  1:3502699/1‑93 (MQ=255)
                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                   <  2:2271690/93‑1 (MQ=255)
                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                   >  2:2135207/1‑93 (MQ=255)
                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                   >  2:141872/1‑93 (MQ=255)
                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                   >  1:3141051/1‑93 (MQ=255)
                                                                      tCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCgaga                   >  2:6065280/1‑93 (MQ=255)
                                                                       cAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGAt                  <  2:4381390/93‑1 (MQ=255)
                                                                         gCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAc                <  2:1279481/93‑1 (MQ=255)
                                                                         gCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATAc                <  2:695565/93‑1 (MQ=255)
                                                                           aTCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCg              <  2:1762580/93‑1 (MQ=255)
                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa             >  2:2563729/1‑93 (MQ=255)
                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa             <  1:4472535/93‑1 (MQ=255)
                                                                            tCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGa             <  2:5175832/93‑1 (MQ=255)
                                                                             cACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGAc            <  1:3221926/93‑1 (MQ=255)
                                                                               cGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGCTGGTATTGCTCCAGTATGGCATCGAGCTACCGACAg          >  2:2759915/1‑93 (MQ=255)
                                                                                ggTTGTGGGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGt         >  2:5105711/1‑93 (MQ=255)
                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt        <  1:3726463/93‑1 (MQ=255)
                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt        <  2:675315/93‑1 (MQ=255)
                                                                                 gTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGtt        <  1:1136633/93‑1 (MQ=255)
                                                                                  ttGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTg       <  1:5583281/93‑1 (MQ=255)
                                                                                  ttGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTg       <  2:2167342/93‑1 (MQ=255)
                                                                                   tgtgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTgg      <  1:2912856/93‑1 (MQ=255)
                                                                                     tgtgCGTGGCGGCGGGCAATACGATACGCCAGACGGTTATCCCGCCCCTGATGGTATTGCTCGAGTAGGGCATCGAGATACCGACAGTTGGcc    <  1:4434045/93‑1 (MQ=255)
                                                                                     tgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGcc    <  1:5599617/93‑1 (MQ=255)
                                                                                     tgtgCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGcc    <  1:6043391/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTC  >  NC_000913/1021286‑1021465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  NC_000913/1021283‑1021469
                                                                                             |                                                                                             
CCGAAACGCTGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCG                                                                                               <  SRR4302533.708343/94‑1 (MQ=60)
  GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTC                                                                                             >  SRR4302533.5087345/1‑94 (MQ=60)
  GAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTC                                                                                             >  SRR4302533.3147329/1‑94 (MQ=60)
   AAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCG                                                                                            <  SRR4302533.4839567/94‑1 (MQ=60)
    AACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGC                                                                                           >  SRR4302533.5285031/1‑94 (MQ=60)
     ACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCG                                                                                          <  SRR4302533.2263047/94‑1 (MQ=60)
      CGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGG                                                                                         >  SRR4302533.5220850/1‑94 (MQ=60)
      CGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGG                                                                                         >  SRR4302533.4992988/1‑94 (MQ=60)
      CGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGG                                                                                         >  SRR4302533.4355662/1‑94 (MQ=60)
        CGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCG                                                                                       <  SRR4302533.3205545/94‑1 (MQ=60)
         ctcttccgatctgctgtatGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGG                                                                                      >  SRR4302533.3848707/20‑94 (MQ=60)
               GCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATA                                                                                >  SRR4302533.6270563/1‑94 (MQ=60)
               GCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATA                                                                                <  SRR4302533.5432566/94‑1 (MQ=60)
                CGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATAC                                                                               <  SRR4302533.2223447/94‑1 (MQ=60)
                 GAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATCCCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACG                                                                              <  SRR4302533.1612557/94‑1 (MQ=60)
                 GAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACG                                                                              >  SRR4302533.658077/1‑94 (MQ=60)
                  AATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGA                                                                             <  SRR4302533.2033494/94‑1 (MQ=60)
                   ATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGAT                                                                            <  SRR4302533.169466/94‑1 (MQ=60)
                   ATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGAT                                                                            <  SRR4302533.3567803/94‑1 (MQ=60)
                      TGCTGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACG                                                                         <  SRR4302533.2680295/94‑1 (MQ=60)
                        CGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCC                                                                       >  SRR4302533.70350/1‑94 (MQ=60)
                         GGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCA                                                                      <  SRR4302533.4467959/94‑1 (MQ=60)
                         GGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCA                                                                      <  SRR4302533.1537609/94‑1 (MQ=60)
                         GGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAACTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCA                                                                      >  SRR4302533.2133841/1‑94 (MQ=60)
                            GTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGAC                                                                   <  SRR4302533.35269/94‑1 (MQ=60)
                            GTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGAC                                                                   <  SRR4302533.2057079/94‑1 (MQ=60)
                             TAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACG                                                                  <  SRR4302533.448718/94‑1 (MQ=60)
                             TAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACG                                                                  <  SRR4302533.5712838/94‑1 (MQ=60)
                             TAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACG                                                                  <  SRR4302533.567467/94‑1 (MQ=60)
                              AACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGG                                                                 >  SRR4302533.1773318/1‑94 (MQ=60)
                              AACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGG                                                                 <  SRR4302533.6181386/94‑1 (MQ=60)
                              AACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGG                                                                 >  SRR4302533.1357539/1‑94 (MQ=60)
                                    CGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCA                                                           <  SRR4302533.4128997/94‑1 (MQ=60)
                                     GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302533.1863843/94‑1 (MQ=60)
                                     GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          <  SRR4302533.2778191/94‑1 (MQ=60)
                                     GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCAC                                                          >  SRR4302533.110798/1‑94 (MQ=60)
                                     GGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGGTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATTAC                                                          >  SRR4302533.4319635/1‑94 (MQ=60)
                                      GCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACG                                                         >  SRR4302533.3074757/1‑94 (MQ=60)
                                       CTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGC                                                        >  SRR4302533.1002992/1‑94 (MQ=60)
                                        TCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCC                                                       <  SRR4302533.5202181/94‑1 (MQ=60)
                                         CGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCC                                                      <  SRR4302533.5173303/94‑1 (MQ=60)
                                          GCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCC                                                     <  SRR4302533.5909775/94‑1 (MQ=60)
                                          GCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCC                                                     >  SRR4302533.5022725/1‑94 (MQ=60)
                                          GCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCC                                                     <  SRR4302533.3981472/94‑1 (MQ=60)
                                            ggaACATATTTGATCCCACCGACGCCGGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTG                                                   <  SRR4302533.2285637/91‑1 (MQ=60)
                                            TGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTG                                                   >  SRR4302533.3177235/1‑94 (MQ=60)
                                             GGATATATTTAATCCCACCGACGCCAGCTCAGCATCCCGGTGTTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGA                                                  <  SRR4302533.1517388/94‑1 (MQ=60)
                                              GACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGAT                                                 <  SRR4302533.3328547/94‑1 (MQ=60)
                                               ACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATG                                                <  SRR4302533.5997030/94‑1 (MQ=60)
                                               ACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATG                                                <  SRR4302533.5448167/94‑1 (MQ=60)
                                                 ATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGT                                              >  SRR4302533.4092323/1‑94 (MQ=60)
                                                   ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            >  SRR4302533.5698926/1‑94 (MQ=60)
                                                   ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            <  SRR4302533.6050553/94‑1 (MQ=60)
                                                   ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            <  SRR4302533.2083991/94‑1 (MQ=60)
                                                   ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            <  SRR4302533.5036119/94‑1 (MQ=60)
                                                   ATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTAT                                            <  SRR4302533.38888/94‑1 (MQ=60)
                                                      TGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGC                                         >  SRR4302533.1660273/1‑94 (MQ=60)
                                                          TCCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCA                                     <  SRR4302533.3699254/94‑1 (MQ=60)
                                                           CCACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAG                                    >  SRR4302533.347785/1‑94 (MQ=60)
                                                            CACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGT                                   >  SRR4302533.2263134/1‑94 (MQ=60)
                                                            CACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGGTATCACGCCCCTGATGGTATTGCTCCAGT                                   >  SRR4302533.3178324/1‑94 (MQ=60)
                                                             ACCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTA                                  >  SRR4302533.3842269/1‑94 (MQ=60)
                                                              CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATGCGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 <  SRR4302533.5627984/94‑1 (MQ=60)
                                                              CCGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTAT                                 <  SRR4302533.1732567/94‑1 (MQ=60)
                                                               CGACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATG                                >  SRR4302533.52248/1‑94 (MQ=60)
                                                                GACGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGG                               >  SRR4302533.4574808/1‑94 (MQ=60)
                                                                  CGCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCA                             <  SRR4302533.5749888/94‑1 (MQ=60)
                                                                   GCCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCAT                            >  SRR4302533.2408719/1‑94 (MQ=60)
                                                                    CCAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATC                           >  SRR4302533.3979217/1‑94 (MQ=60)
                                                                     CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          <  SRR4302533.382969/94‑1 (MQ=60)
                                                                     CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          >  SRR4302533.3524295/1‑94 (MQ=60)
                                                                     CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          <  SRR4302533.3349293/94‑1 (MQ=60)
                                                                     CAGCTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCACACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCG                          <  SRR4302533.4462941/94‑1 (MQ=60)
                                                                        CTCAGCATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGA                       >  SRR4302533.3160361/1‑94 (MQ=60)
                                                                             CATCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCG                  >  SRR4302533.815887/1‑94 (MQ=60)
                                                                               TCACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGAC                <  SRR4302533.4499587/94‑1 (MQ=60)
                                                                                CACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACA               <  SRR4302533.3241796/94‑1 (MQ=60)
                                                                                 ACGGTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAG              <  SRR4302533.2925903/94‑1 (MQ=60)
                                                                                    GTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTG           <  SRR4302533.5116780/94‑1 (MQ=60)
                                                                                    GTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTG           <  SRR4302533.3749323/94‑1 (MQ=60)
                                                                                    GTTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTG           <  SRR4302533.1143392/94‑1 (MQ=60)
                                                                                     TTGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGG          <  SRR4302533.5617017/94‑1 (MQ=60)
                                                                                      TGTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGC         <  SRR4302533.2930731/94‑1 (MQ=60)
                                                                                       GTGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCC        >  SRR4302533.301401/1‑94 (MQ=60)
                                                                                        TGTGCGTGGCGGCGGGCAATACGATACGCCAGACGGTTATCCCGCCCCTGATGGTATTGCTCGAGTAGGGCATCGAGATACCGACAGTTGGCCT       <  SRR4302533.4460853/94‑1 (MQ=60)
                                                                                        TGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCT       <  SRR4302533.5633449/94‑1 (MQ=60)
                                                                                        TGTGCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCT       <  SRR4302533.6080004/94‑1 (MQ=60)
                                                                                           GCGTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTG    <  SRR4302533.4845680/94‑1 (MQ=60)
                                                                                             GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  <  SRR4302533.5380808/94‑1 (MQ=60)
                                                                                             GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  <  SRR4302533.386791/94‑1 (MQ=60)
                                                                                             GTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  SRR4302533.1591655/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGAAACGCGGCTTCGCGAATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTG  >  NC_000913/1021283‑1021469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑