Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,672	A→G	K170K (AAA→AAG)	yceC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,672	0	A	G	100.0%	930.4 / NA	284	K170K (AAA→AAG)	yceC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (131/153); total (131/153)

Only 100 of 302 total aligned reads displayed.

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  NC_000913/1144582‑1144764

tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                            >  1:2875038/1‑93 (MQ=255)

 cATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAgggg                                                                                           <  2:2416514/93‑1 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                       >  1:9373419/1‑93 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                       >  2:258011/1‑93 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                      <  2:9615870/93‑1 (MQ=255)

        ttGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaa                                                                                    <  2:3598930/93‑1 (MQ=255)

          gACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaaaa                                                                                  >  1:1194998/1‑93 (MQ=255)

           aCCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAg                                                                                 <  2:3015217/93‑1 (MQ=255)

             cGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAt                                                                               >  2:5760590/1‑93 (MQ=255)

             cGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAt                                                                               <  2:601160/93‑1 (MQ=255)

              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                              <  1:3701138/93‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                             <  1:4660481/93‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                             <  2:5849620/93‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                             <  2:6232206/93‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                             <  2:1400853/93‑1 (MQ=255)

                gACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAc                                                                            <  1:5572024/93‑1 (MQ=255)

                gACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAc                                                                            <  1:7583430/93‑1 (MQ=255)

                 gcacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAcc                                                                           <  1:8460241/92‑1 (MQ=255)

                 acacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAcc                                                                           <  2:6505874/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                          <  1:4007757/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                          >  1:2020392/1‑93 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAACTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                          <  2:508901/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACCCTCGGCGTTGCGTTCTCTCCATGACCAATTATGTGCAAAGGGGATGTACAACGATGACCt                                                                          >  1:8711594/1‑93 (MQ=255)

                   tccgcAGGTGTTGTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                         <  2:4121573/89‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                         <  1:4490968/93‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGAATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                         >  2:5205865/1‑93 (MQ=255)

                    ccTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTgg                                                                        <  2:9484207/93‑1 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                       >  2:3416546/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                       >  2:3263340/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                       >  1:5434526/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                       <  2:1660148/93‑1 (MQ=255)

                      tCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcg                                                                      >  2:7500579/1‑93 (MQ=255)

                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                    <  2:7234774/93‑1 (MQ=255)

                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                   >  2:9409525/1‑93 (MQ=255)

                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                   >  1:9150528/1‑93 (MQ=255)

                          gtgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTgg                                                                  >  1:3589619/1‑93 (MQ=255)

                          gtgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTgg                                                                  >  1:3933494/1‑93 (MQ=255)

                           tgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGt                                                                 <  1:1128346/93‑1 (MQ=255)

                            gtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTg                                                                >  1:8614353/1‑93 (MQ=255)

                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                               <  2:6630846/93‑1 (MQ=255)

                             gtttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                               <  1:381508/92‑1 (MQ=255)

                               ttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcgc                                                             <  1:6485990/93‑1 (MQ=255)

                               ttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcgc                                                             <  1:4306142/93‑1 (MQ=255)

                                   tGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTc                                                         <  2:4387614/93‑1 (MQ=255)

                                    ggTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCa                                                        <  2:2707702/93‑1 (MQ=255)

                                     gTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAg                                                       <  1:7771238/93‑1 (MQ=255)

                                     gTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAg                                                       >  1:1454628/1‑93 (MQ=255)

                                     gTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAg                                                       <  2:128087/93‑1 (MQ=255)

                                     gTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGAGCGGTCAg                                                       >  2:2673699/1‑93 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                      <  1:1391394/93‑1 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                      <  1:3104340/93‑1 (MQ=255)

                                        gCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTgg                                                    <  2:3853552/93‑1 (MQ=255)

                                         cGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGc                                                   >  2:8383743/1‑93 (MQ=255)

                                         cGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGc                                                   <  1:172444/93‑1 (MQ=255)

                                           aaaaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAg                                                 >  2:7947572/1‑93 (MQ=255)

                                           aaaaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAg                                                 <  2:6857057/93‑1 (MQ=255)

                                            aaaaaCACTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGt                                                <  1:7790057/93‑1 (MQ=255)

                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                               <  1:8084172/93‑1 (MQ=255)

                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                               <  1:6828237/93‑1 (MQ=255)

                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCg                                              >  2:7379684/1‑93 (MQ=255)

                                                aCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCa                                            <  1:2347005/93‑1 (MQ=255)

                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                          <  2:2858137/93‑1 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                         >  1:6265261/1‑93 (MQ=255)

                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                        <  1:706439/93‑1 (MQ=255)

                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                        >  2:2635172/1‑93 (MQ=255)

                                                      ggCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGaa                                      <  1:5748674/93‑1 (MQ=255)

                                                      ggCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGaa                                      <  2:6109162/93‑1 (MQ=255)

                                                       gcgttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAag                                     >  1:8815998/1‑93 (MQ=255)

                                                       gcgttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAAGTGAag                                     >  2:8973313/1‑93 (MQ=255)

                                                         gttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAagag                                   >  1:572609/1‑93 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                                  >  2:2165293/1‑93 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                                  >  1:9262935/1‑93 (MQ=255)

                                                            gcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                                <  1:1069771/93‑1 (MQ=255)

                                                                tctcTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCaa                            >  1:4904854/1‑93 (MQ=255)

                                                                  tctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGc                          <  2:3076920/93‑1 (MQ=255)

                                                                  tctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGc                          >  2:5444281/1‑93 (MQ=255)

                                                                  tctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGGTCAAga                          >  2:4517933/1‑92 (MQ=255)

                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                         <  1:2724638/93‑1 (MQ=255)

                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                         <  2:2040169/93‑1 (MQ=255)

                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                         <  2:7675477/93‑1 (MQ=255)

                                                                     gCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAcc                       <  2:6981354/93‑1 (MQ=255)

                                                                     gCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAcc                       >  2:9281651/1‑93 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGC‑AAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                    <  2:1607652/93‑1 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATGACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                    >  2:6375868/1‑93 (MQ=255)

                                                                         gAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTa                   >  2:5672431/1‑93 (MQ=255)

                                                                           gCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACt                 <  1:9539685/93‑1 (MQ=255)

                                                                            cAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTg                >  1:9313955/1‑93 (MQ=255)

                                                                            cAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTg                >  2:2281141/1‑93 (MQ=255)

                                                                             aaTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGCAGAGCGTTCAAGCACCGTTACTGa               >  1:5205574/1‑93 (MQ=255)

                                                                             aaTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGa               <  2:1932985/93‑1 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa              >  2:6741646/1‑93 (MQ=255)

                                                                               ttACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaa             >  1:1572931/1‑93 (MQ=255)

                                                                               ttACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaa             >  2:8208222/1‑93 (MQ=255)

                                                                                tACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaa            <  1:5073277/93‑1 (MQ=255)

                                                                                 aCGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa           <  2:9167858/93‑1 (MQ=255)

                                                                                   gTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAata         <  2:9716357/93‑1 (MQ=255)

                                                                                    tGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAatat        <  2:4769099/93‑1 (MQ=255)

                                                                                      aaaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc      <  1:7309221/93‑1 (MQ=255)

                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt     >  1:9030859/1‑93 (MQ=255)

                                                                                          aGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCa  <  1:3342050/93‑1 (MQ=255)

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  NC_000913/1144582‑1144764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCAA  >  NC_000913/1144579‑1144765

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                               <  SRR4302540.9917423/94‑1 (MQ=60)

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                               <  SRR4302540.3177432/94‑1 (MQ=60)

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                               <  SRR4302540.3470309/94‑1 (MQ=60)

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                               <  SRR4302540.1007632/94‑1 (MQ=60)

 GTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGG                                                                                              >  SRR4302540.2077812/1‑94 (MQ=60)

 GTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGG                                                                                              <  SRR4302540.1881281/94‑1 (MQ=60)

 GTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGG                                                                                              >  SRR4302540.3461919/1‑94 (MQ=60)

 GTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGG                                                                                              >  SRR4302540.4269637/1‑94 (MQ=60)

  TTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGG                                                                                             <  SRR4302540.6610989/94‑1 (MQ=60)

  TTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGG                                                                                             >  SRR4302540.2906295/1‑94 (MQ=60)

  TTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGG                                                                                             >  SRR4302540.9868238/1‑94 (MQ=60)

   TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGG                                                                                            <  SRR4302540.4008816/94‑1 (MQ=60)

    CATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGA                                                                                           >  SRR4302540.9578068/1‑94 (MQ=60)

     ATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGAT                                                                                          >  SRR4302540.6450764/1‑94 (MQ=60)

      TCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATG                                                                                         <  SRR4302540.7598374/94‑1 (MQ=60)

      TCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATG                                                                                         <  SRR4302540.3897757/94‑1 (MQ=60)

       CGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGC                                                                                        >  SRR4302540.945490/1‑94 (MQ=60)

       CGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGC                                                                                        >  SRR4302540.9485010/1‑94 (MQ=60)

          CTTGACCGGGACACCTCAGGAGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGCTCTCTGCATGAGCAATTACGTGAACAGGGGAGGCAAA                                                                                     >  SRR4302540.1103201/1‑94 (MQ=60)

            TGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAA                                                                                   >  SRR4302540.1207304/1‑94 (MQ=60)

            TGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAA                                                                                   >  SRR4302540.7694220/1‑94 (MQ=60)

              ACCGGGCCGCCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGA                                                                                 <  SRR4302540.5461234/94‑1 (MQ=60)

              ACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGA                                                                                 >  SRR4302540.4389428/1‑94 (MQ=60)

               CCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAT                                                                                <  SRR4302540.5938721/94‑1 (MQ=60)

               CCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAT                                                                                <  SRR4302540.5243136/94‑1 (MQ=60)

               CCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTGCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAT                                                                                <  SRR4302540.4566555/94‑1 (MQ=60)

                CGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATT                                                                               <  SRR4302540.3365447/94‑1 (MQ=60)

                 GGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTA                                                                              <  SRR4302540.9195636/94‑1 (MQ=60)

                 GGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTA                                                                              <  SRR4302540.3741543/94‑1 (MQ=60)

                 GGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTA                                                                              >  SRR4302540.1114466/1‑94 (MQ=60)

                 GGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTA                                                                              >  SRR4302540.9207522/1‑94 (MQ=60)

                  GGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAC                                                                             <  SRR4302540.4710367/94‑1 (MQ=60)

                  GGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAC                                                                             <  SRR4302540.7891336/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.9021509/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.1590262/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.5633376/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.5633377/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.7668568/94‑1 (MQ=60)

                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                            <  SRR4302540.6687158/94‑1 (MQ=60)

                    GCACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           <  SRR4302540.8558469/94‑1 (MQ=60)

                    ACCCCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           <  SRR4302540.1461810/94‑1 (MQ=60)

                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           <  SRR4302540.1461813/94‑1 (MQ=60)

                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           <  SRR4302540.405898/94‑1 (MQ=60)

                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           >  SRR4302540.8812727/1‑94 (MQ=60)

                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           <  SRR4302540.7500698/94‑1 (MQ=60)

                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                           >  SRR4302540.2042233/1‑94 (MQ=60)

                     CACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTG                                                                          <  SRR4302540.5393931/94‑1 (MQ=60)

                     CACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTG                                                                          <  SRR4302540.4050906/94‑1 (MQ=60)

                     CACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTG                                                                          <  SRR4302540.4039596/94‑1 (MQ=60)

                      ACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                         <  SRR4302540.4539663/94‑1 (MQ=60)

                       CCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGC                                                                        >  SRR4302540.5494297/1‑94 (MQ=60)

                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                       >  SRR4302540.3806385/1‑94 (MQ=60)

                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                       >  SRR4302540.5557829/1‑94 (MQ=60)

                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCCCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                       >  SRR4302540.1778366/1‑94 (MQ=60)

                         TCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGC                                                                      >  SRR4302540.7817593/1‑94 (MQ=60)

                           AGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTG                                                                    >  SRR4302540.9260406/1‑94 (MQ=60)

                            GGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGG                                                                   >  SRR4302540.3976013/1‑94 (MQ=60)

                            GGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGG                                                                   >  SRR4302540.3439436/1‑94 (MQ=60)

                            GGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGG                                                                   >  SRR4302540.3628830/1‑94 (MQ=60)

                              TGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTG                                                                 <  SRR4302540.1140085/94‑1 (MQ=60)

                              TGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTG                                                                 >  SRR4302540.8714443/1‑94 (MQ=60)

                                GTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCG                                                               <  SRR4302540.385451/94‑1 (MQ=60)

                                 TTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGC                                                              <  SRR4302540.6860080/94‑1 (MQ=60)

                                  TTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCG                                                             >  SRR4302540.5112817/1‑94 (MQ=60)

                                  TTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCG                                                             <  SRR4302540.4353010/94‑1 (MQ=60)

                                  TTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCG                                                             <  SRR4302540.6558120/94‑1 (MQ=60)

                                  TTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCG                                                             <  SRR4302540.6558121/94‑1 (MQ=60)

                                    GCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGT                                                           >  SRR4302540.9379284/1‑94 (MQ=60)

                                     CTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATCAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGGGCGCGGTC                                                          >  SRR4302540.8450506/1‑94 (MQ=60)

                                       GGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAG                                                        >  SRR4302540.1470449/1‑94 (MQ=60)

                                        GTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGT                                                       <  SRR4302540.7862091/94‑1 (MQ=60)

                                        GTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGT                                                       <  SRR4302540.7862093/94‑1 (MQ=60)

                                        GTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGT                                                       >  SRR4302540.5048378/1‑94 (MQ=60)

                                        GTAGCGAAAAAACGCTCGGCGTTGCGTTCTCGGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGT                                                       <  SRR4302540.7862095/94‑1 (MQ=60)

                                         TAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTG                                                      <  SRR4302540.1406288/94‑1 (MQ=60)

                                         TAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTG                                                      <  SRR4302540.3138329/94‑1 (MQ=60)

                                          AGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGG                                                     <  SRR4302540.9478735/94‑1 (MQ=60)

                                           GCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGC                                                    >  SRR4302540.3231494/1‑94 (MQ=60)

                                            CGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCA                                                   <  SRR4302540.174164/94‑1 (MQ=60)

                                             GAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAG                                                  >  SRR4302540.1300689/1‑94 (MQ=60)

                                             GAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAG                                                  >  SRR4302540.2131475/1‑94 (MQ=60)

                                             GAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAG                                                  <  SRR4302540.4339565/94‑1 (MQ=60)

                                              AAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGT                                                 >  SRR4302540.9405536/1‑94 (MQ=60)

                                               AAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTC                                                >  SRR4302540.4935874/1‑94 (MQ=60)

                                               AAAAACACTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTC                                                <  SRR4302540.7881116/94‑1 (MQ=60)

                                                AAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCG                                               >  SRR4302540.1823566/1‑94 (MQ=60)

                                                AAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCG                                               <  SRR4302540.8178000/94‑1 (MQ=60)

                                                AAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCG                                               <  SRR4302540.6903841/94‑1 (MQ=60)

                                                 AAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGC                                              >  SRR4302540.7328835/1‑94 (MQ=60)

                                                 AAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGC                                              >  SRR4302540.7328836/1‑94 (MQ=60)

                                                   ACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAT                                            <  SRR4302540.2372288/94‑1 (MQ=60)

                                                    CGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATG                                           <  SRR4302540.7932388/94‑1 (MQ=60)

                                                     GCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGT                                          <  SRR4302540.2886126/94‑1 (MQ=60)

                                                     GCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGT                                          >  SRR4302540.6334959/1‑94 (MQ=60)

                                                      CTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTG                                         <  SRR4302540.3812044/94‑1 (MQ=60)

                                                      CTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGGCGCATGTG                                         >  SRR4302540.8802450/1‑94 (MQ=60)

                                                       TCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGA                                        <  SRR4302540.714027/94‑1 (MQ=60)

                                                       TCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGA                                        <  SRR4302540.3693499/94‑1 (MQ=60)

                                                       TCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGA                                        >  SRR4302540.562808/1‑94 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGANCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      <  SRR4302540.1384875/94‑1 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      <  SRR4302540.5812095/94‑1 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      >  SRR4302540.8917929/1‑94 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      <  SRR4302540.1681116/94‑1 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      <  SRR4302540.5812093/94‑1 (MQ=60)

                                                         GGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAG                                      <  SRR4302540.3109026/94‑1 (MQ=60)

                                                          GCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGA                                     <  SRR4302540.1534411/94‑1 (MQ=60)

                                                           CGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAG                                    >  SRR4302540.5131722/1‑94 (MQ=60)

                                                           CGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAG                                    >  SRR4302540.578825/1‑94 (MQ=60)

                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                   <  SRR4302540.9355639/94‑1 (MQ=60)

                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                   >  SRR4302540.9373740/1‑94 (MQ=60)

                                                              TGCGTTCTCTGCATGAGCAATTACGTGCAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGT                                 >  SRR4302540.3736294/1‑94 (MQ=60)

                                                              TGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGT                                 >  SRR4302540.2986603/1‑94 (MQ=60)

                                                               TCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                                <  SRR4302540.8830291/94‑1 (MQ=60)

                                                               GCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                                <  SRR4302540.1197824/94‑1 (MQ=60)

                                                               GCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                                <  SRR4302540.1080676/94‑1 (MQ=60)

                                                                 GTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCA                              >  SRR4302540.5675291/1‑94 (MQ=60)

                                                                 GTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCA                              <  SRR4302540.2466547/94‑1 (MQ=60)

                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                             >  SRR4302540.4957633/1‑94 (MQ=60)

                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                             <  SRR4302540.1487607/94‑1 (MQ=60)

                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                             >  SRR4302540.8726844/1‑94 (MQ=60)

                                                                     TCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCA                          >  SRR4302540.3934164/1‑94 (MQ=60)

                                                                      CTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAC                         <  SRR4302540.2754020/94‑1 (MQ=60)

                                                                       TGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACC                        <  SRR4302540.1112810/94‑1 (MQ=60)

                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGTTTACCTGGCGCTGGTGCGCGGTCAGTTGCAGTCGCATGTGAACATCGTTCAAGCACCGTT                     >  SRR4302540.9075906/1‑94 (MQ=60)

                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                     >  SRR4302540.2653383/1‑94 (MQ=60)

                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                     <  SRR4302540.9348172/94‑1 (MQ=60)

                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                     >  SRR4302540.7029270/1‑94 (MQ=60)

                                                                             AGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACT                  >  SRR4302540.1174230/1‑94 (MQ=60)

                                                                             AGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACT                  <  SRR4302540.578942/94‑1 (MQ=60)

                                                                              GCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTG                 <  SRR4302540.9652579/94‑1 (MQ=60)

                                                                              GCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTG                 >  SRR4302540.9425121/1‑94 (MQ=60)

                                                                               CAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGCAGAGCGTTCAAGCACCGTTACTGA                >  SRR4302540.5262274/1‑94 (MQ=60)

                                                                               CAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGA                >  SRR4302540.7098625/1‑94 (MQ=60)

                                                                               CAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGA                <  SRR4302540.8288945/94‑1 (MQ=60)

                                                                                AATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAA               >  SRR4302540.8607349/1‑94 (MQ=60)

                                                                                AATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAA               >  SRR4302540.3382871/1‑94 (MQ=60)

                                                                                 ATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAA              >  SRR4302540.602071/1‑94 (MQ=60)

                                                                                 ATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAA              >  SRR4302540.4576605/1‑94 (MQ=60)

                                                                                 ATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAA              >  SRR4302540.1590159/1‑94 (MQ=60)

                                                                                  TTACATGAAAAGGGGATGCAAAAAGTTTGCCGGGCGCTGGTGCGCTGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAA             <  SRR4302540.4768772/94‑1 (MQ=60)

                                                                                   TACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAA            <  SRR4302540.5128123/94‑1 (MQ=60)

                                                                                     CGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATA          >  SRR4302540.4395728/1‑94 (MQ=60)

                                                                                     CGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATA          >  SRR4302540.8626137/1‑94 (MQ=60)

                                                                                      GTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAT         >  SRR4302540.9594135/1‑94 (MQ=60)

                                                                                       TGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATT        >  SRR4302540.2050156/1‑94 (MQ=60)

                                                                                         AAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT      >  SRR4302540.9139236/1‑94 (MQ=60)

                                                                                         AAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT      <  SRR4302540.7390821/94‑1 (MQ=60)

                                                                                          AAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTG     >  SRR4302540.9247074/1‑94 (MQ=60)

                                                                                             GGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCAA  <  SRR4302540.7746349/94‑1 (MQ=60)

                                                                                             GGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCAA  >  SRR4302540.8909672/1‑94 (MQ=60)

                                                                                             AGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCAA  <  SRR4302540.3378723/94‑1 (MQ=60)

GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCAA  >  NC_000913/1144579‑1144765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`