Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,144,672 A→G K170K (AAA→AAG)  yceC → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,144,6720AG99.5% 608.6 / ‑4.9 190K170K (AAA→AAG) yceChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (102/87);  total (102/88)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 208 total aligned reads displayed.

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT  >  NC_000913/1144582‑1144761
                                                                                          |                                                                                         
tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                         <  2:5930339/93‑1 (MQ=255)
tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                         <  1:4794528/93‑1 (MQ=255)
 cATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGGTCTCTGCATGAGCAATTACGTGAAAAgggg                                                                                        >  1:2569539/1‑93 (MQ=255)
     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                    >  1:111318/1‑93 (MQ=255)
      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   >  1:1145110/1‑93 (MQ=255)
      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   >  2:276417/1‑93 (MQ=255)
      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   <  2:3323773/93‑1 (MQ=255)
      gcTTGACCGGGACGCCTCAGGTGCGTTGCTGGTAACGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   <  1:4356809/92‑1 (MQ=255)
       cTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaa                                                                                  >  2:4287502/1‑93 (MQ=255)
        ttGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaa                                                                                 >  2:5654673/1‑93 (MQ=255)
        ttGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaa                                                                                 <  2:5282788/93‑1 (MQ=255)
           aCCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAg                                                                              >  1:5520810/1‑93 (MQ=255)
              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCCAAAAGAtt                                                                           <  2:6134618/93‑1 (MQ=255)
              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                           >  1:6275370/1‑93 (MQ=255)
               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                          >  2:5033503/1‑93 (MQ=255)
                 acacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAcc                                                                        <  2:5936779/93‑1 (MQ=255)
                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                       <  2:4038202/93‑1 (MQ=255)
                   acCTCAGGTGTTTTGCTGGTAGCTAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  1:954852/93‑1 (MQ=255)
                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  1:4642111/93‑1 (MQ=255)
                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  2:2393838/93‑1 (MQ=255)
                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      >  2:1147829/1‑93 (MQ=255)
                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      >  1:1907805/1‑93 (MQ=255)
                    ccTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTgg                                                                     >  1:791735/1‑93 (MQ=255)
                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                    <  2:6122205/93‑1 (MQ=255)
                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                    <  1:4574038/93‑1 (MQ=255)
                       cAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcgc                                                                  <  1:166292/93‑1 (MQ=255)
                       cAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcgc                                                                  >  1:720970/1‑93 (MQ=255)
                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGTCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  2:1401562/1‑93 (MQ=255)
                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  1:2865076/1‑93 (MQ=255)
                        aGGTGTTTTGCTGCTAGCCAAAAAACGCTCGGCGCTGCGTTCTCTGCATGAGCAATTACGTGTAAAGGGGATGAAAAAAGCTTACCTGGCGCt                                                                 >  2:1600388/1‑93 (MQ=255)
                        aGGGGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGGGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  2:2291921/1‑93 (MQ=255)
                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  1:3230193/1‑93 (MQ=255)
                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  2:2877707/1‑93 (MQ=255)
                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  2:3915/1‑93 (MQ=255)
                          gtgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTgg                                                               >  2:1630480/1‑93 (MQ=255)
                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                            <  2:1340480/93‑1 (MQ=255)
                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                            <  1:5256824/93‑1 (MQ=255)
                               ttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcgc                                                          >  1:4038343/1‑93 (MQ=255)
                                  cTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGt                                                       <  1:980742/93‑1 (MQ=255)
                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  2:3260205/93‑1 (MQ=255)
                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   >  2:317943/1‑93 (MQ=255)
                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  1:4827339/93‑1 (MQ=255)
                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  2:5175122/93‑1 (MQ=255)
                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  1:3260853/93‑1 (MQ=255)
                                        gCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTgg                                                 <  2:2434545/93‑1 (MQ=255)
                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                            >  1:5905683/1‑93 (MQ=255)
                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                            <  1:4263284/93‑1 (MQ=255)
                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCg                                           >  2:353990/1‑93 (MQ=255)
                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCg                                           >  1:6130728/1‑93 (MQ=255)
                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          >  1:5760506/1‑93 (MQ=255)
                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          >  1:5833954/1‑93 (MQ=255)
                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          <  1:221442/93‑1 (MQ=255)
                                                 cGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAt                                        <  2:5329226/93‑1 (MQ=255)
                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                       <  1:2460166/93‑1 (MQ=255)
                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                       >  2:2250901/1‑93 (MQ=255)
                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGCTTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                      >  2:6011367/1‑93 (MQ=255)
                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                      >  2:3658765/1‑93 (MQ=255)
                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgg                                      >  2:315077/1‑92 (MQ=255)
                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                     >  2:424905/1‑93 (MQ=255)
                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                     >  2:2849169/1‑93 (MQ=255)
                                                       gcgttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAag                                  <  1:2045543/93‑1 (MQ=255)
                                                         gttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAagag                                <  1:3195517/93‑1 (MQ=255)
                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAagagg                               >  2:4387421/1‑92 (MQ=255)
                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                               >  2:584158/1‑93 (MQ=255)
                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                               >  2:467303/1‑93 (MQ=255)
                                                            tcgtcCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                             <  2:2556829/92‑1 (MQ=255)
                                                            gcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                             <  1:3836508/93‑1 (MQ=255)
                                                             cgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGtt                            >  1:2942101/1‑93 (MQ=255)
                                                               ttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCa                          >  1:2955092/1‑93 (MQ=255)
                                                               ttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCa                          <  1:1782425/93‑1 (MQ=255)
                                                                tctcTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCaa                         <  2:2206506/93‑1 (MQ=255)
                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                      >  2:5007404/1‑93 (MQ=255)
                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                      >  1:5093344/1‑93 (MQ=255)
                                                                    tGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAc                     <  1:4713689/93‑1 (MQ=255)
                                                                     gCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAcc                    <  2:1727329/93‑1 (MQ=255)
                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  1:1554/1‑93 (MQ=255)
                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:814646/1‑93 (MQ=255)
                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:3083562/1‑93 (MQ=255)
                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  1:3986430/1‑93 (MQ=255)
                                                                        tGAGCAATTACGTGAAAAGGGGATACAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:4802565/1‑93 (MQ=255)
                                                                         gAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTa                >  1:5332838/1‑93 (MQ=255)
                                                                          aGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTAc               >  1:2368288/1‑93 (MQ=255)
                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  2:759485/1‑93 (MQ=255)
                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  1:3085405/1‑93 (MQ=255)
                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  1:4978858/1‑93 (MQ=255)
                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGCTCAAGCACCGTTACTGaa           >  2:6305806/1‑93 (MQ=255)
                                                                                tACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaa         <  1:5922571/93‑1 (MQ=255)
                                                                                 aCGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        >  2:2116198/1‑93 (MQ=255)
                                                                                 aCGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        <  1:3853450/93‑1 (MQ=255)
                                                                                 aCGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        <  1:3457962/93‑1 (MQ=255)
                                                                                  cGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAat       <  1:2983392/93‑1 (MQ=255)
                                                                                   gTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAata      <  2:1742899/93‑1 (MQ=255)
                                                                                    tGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAatat     <  1:340409/93‑1 (MQ=255)
                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt    <  2:1954971/93‑1 (MQ=255)
                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt    >  1:1414558/1‑93 (MQ=255)
                                                                                      aaaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc   <  2:4567460/93‑1 (MQ=255)
                                                                                      aaaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc   <  2:3745544/93‑1 (MQ=255)
                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  <  1:1835179/93‑1 (MQ=255)
                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  >  1:2743641/1‑93 (MQ=255)
                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  <  1:228562/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT  >  NC_000913/1144582‑1144761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  NC_000913/1144579‑1144764
                                                                                             |                                                                                            
GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                              <  SRR4302533.3180442/94‑1 (MQ=60)
GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAG                                                                                              <  SRR4302533.3175115/94‑1 (MQ=60)
 GTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGG                                                                                             <  SRR4302533.3504560/94‑1 (MQ=60)
  TTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGG                                                                                            <  SRR4302533.5852320/94‑1 (MQ=60)
   TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGG                                                                                           <  SRR4302533.4823572/94‑1 (MQ=60)
   TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGGTCTCTGCATGAGCAATTACGTGAAAAGGGG                                                                                           >  SRR4302533.2585351/1‑94 (MQ=60)
    CATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGA                                                                                          >  SRR4302533.1150332/1‑94 (MQ=60)
     ATCGTCTTGAGCGGGACACCTCAGGTGTTTTGGTGGTAGCGAAAAATCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGAT                                                                                         <  SRR4302533.5377979/94‑1 (MQ=60)
      TCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATG                                                                                        <  SRR4302533.178377/94‑1 (MQ=60)
       CGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGC                                                                                       >  SRR4302533.2750605/1‑94 (MQ=60)
       CGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGC                                                                                       >  SRR4302533.112022/1‑94 (MQ=60)
        GTCTTGACCGGGACACCTCAGGTGTTTTGCTTGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCA                                                                                      >  SRR4302533.483258/1‑94 (MQ=60)
        GTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCA                                                                                      >  SRR4302533.1151945/1‑94 (MQ=60)
        GTCTTGACCGGGACACCTCAGGCGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGGTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCA                                                                                      >  SRR4302533.1984033/1‑94 (MQ=60)
         GCTTGACCGGGACGCCTCAGGTGCGTTGCTGGTAACGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAA                                                                                     <  SRR4302533.4383107/94‑1 (MQ=60)
          CTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAA                                                                                    >  SRR4302533.1813505/1‑94 (MQ=60)
          CTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAA                                                                                    >  SRR4302533.1916137/1‑94 (MQ=60)
             GACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAG                                                                                 <  SRR4302533.2488930/94‑1 (MQ=60)
             GACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAG                                                                                 >  SRR4302533.5554142/1‑94 (MQ=60)
                CGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATT                                                                              <  SRR4302533.1634469/94‑1 (MQ=60)
                CGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATT                                                                              >  SRR4302533.6312844/1‑94 (MQ=60)
                 GGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTA                                                                             >  SRR4302533.5086740/1‑94 (MQ=60)
                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGCTGCAAAAAGATTACC                                                                           >  SRR4302533.3239562/1‑94 (MQ=60)
                   GACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACC                                                                           <  SRR4302533.3115798/94‑1 (MQ=60)
                    ACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCT                                                                          >  SRR4302533.2979985/1‑94 (MQ=60)
                     CACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTG                                                                         >  SRR4302533.1919443/1‑94 (MQ=60)
                      ACCTCAGGTGTTTTGCTGGTAGCTAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                        <  SRR4302533.960459/94‑1 (MQ=60)
                      ACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                        <  SRR4302533.4320599/94‑1 (MQ=60)
                      ACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                        <  SRR4302533.4051530/94‑1 (MQ=60)
                      ACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                        <  SRR4302533.4670235/94‑1 (MQ=60)
                      ACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGG                                                                        >  SRR4302533.796394/1‑94 (MQ=60)
                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                      <  SRR4302533.4601758/94‑1 (MQ=60)
                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                      <  SRR4302533.3474415/94‑1 (MQ=60)
                        CTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCG                                                                      >  SRR4302533.2076992/1‑94 (MQ=60)
                         TCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGC                                                                     >  SRR4302533.725224/1‑94 (MQ=60)
                          CAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCT                                                                    <  SRR4302533.167346/94‑1 (MQ=60)
                          CAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCT                                                                    >  SRR4302533.2882703/1‑94 (MQ=60)
                           AGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTG                                                                   >  SRR4302533.3590674/1‑94 (MQ=60)
                           AGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTG                                                                   >  SRR4302533.3250105/1‑94 (MQ=60)
                                TTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCG                                                              <  SRR4302533.5288395/94‑1 (MQ=60)
                                 TTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGC                                                             >  SRR4302533.4062917/1‑94 (MQ=60)
                                    GCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGT                                                          <  SRR4302533.3861779/94‑1 (MQ=60)
                                     CTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTC                                                         <  SRR4302533.986493/94‑1 (MQ=60)
                                       GGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAG                                                       <  SRR4302533.2384613/94‑1 (MQ=60)
                                         TAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTG                                                     <  SRR4302533.4856593/94‑1 (MQ=60)
                                         TAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTG                                                     <  SRR4302533.3280968/94‑1 (MQ=60)
                                         TAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTG                                                     >  SRR4302533.787089/1‑94 (MQ=60)
                                              AAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGT                                                >  SRR4302533.3058967/1‑94 (MQ=60)
                                              AAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGAGGCAGT                                                >  SRR4302533.2531870/1‑94 (MQ=60)
                                               AAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTC                                               <  SRR4302533.2931361/94‑1 (MQ=60)
                                               AAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTC                                               >  SRR4302533.5941348/1‑94 (MQ=60)
                                                AAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCG                                              <  SRR4302533.4288968/94‑1 (MQ=60)
                                                AAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCG                                              >  SRR4302533.6167670/1‑94 (MQ=60)
                                                 AAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGC                                             >  SRR4302533.5795266/1‑94 (MQ=60)
                                                 AAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGC                                             >  SRR4302533.5869211/1‑94 (MQ=60)
                                                  AACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCA                                            <  SRR4302533.222829/94‑1 (MQ=60)
                                                   ACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAT                                           <  SRR4302533.6089710/94‑1 (MQ=60)
                                                     GCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGT                                         <  SRR4302533.908879/94‑1 (MQ=60)
                                                     GCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGT                                         <  SRR4302533.2475348/94‑1 (MQ=60)
                                                      CTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTG                                        <  SRR4302533.4194982/94‑1 (MQ=60)
                                                        CGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAA                                      <  SRR4302533.997586/94‑1 (MQ=60)
                                                          GCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGA                                    <  SRR4302533.2058153/94‑1 (MQ=60)
                                                          GCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGA                                    >  SRR4302533.5910761/1‑94 (MQ=60)
                                                           CGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAG                                   >  SRR4302533.704539/1‑94 (MQ=60)
                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                  >  SRR4302533.2246503/1‑94 (MQ=60)
                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                  >  SRR4302533.4958118/1‑94 (MQ=60)
                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                  <  SRR4302533.4391607/94‑1 (MQ=60)
                                                            GTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGC                                  <  SRR4302533.3215179/94‑1 (MQ=60)
                                                               GCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                               <  SRR4302533.3859949/94‑1 (MQ=60)
                                                               GCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                               <  SRR4302533.2969200/94‑1 (MQ=60)
                                                               GCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTT                               >  SRR4302533.2960067/1‑94 (MQ=60)
                                                                CGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTC                              >  SRR4302533.232851/1‑94 (MQ=60)
                                                                 GTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCA                             >  SRR4302533.2973162/1‑94 (MQ=60)
                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                            <  SRR4302533.2819553/94‑1 (MQ=60)
                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                            <  SRR4302533.4624593/94‑1 (MQ=60)
                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                            >  SRR4302533.3379946/1‑94 (MQ=60)
                                                                  TTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAA                            <  SRR4302533.1793270/94‑1 (MQ=60)
                                                                   TCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAG                           <  SRR4302533.5853084/94‑1 (MQ=60)
                                                                    CTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGC                          >  SRR4302533.4721633/1‑94 (MQ=60)
                                                                     TCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCA                         >  SRR4302533.5124113/1‑94 (MQ=60)
                                                                      CTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAC                        >  SRR4302533.1431706/1‑94 (MQ=60)
                                                                       TGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACC                       <  SRR4302533.4742258/94‑1 (MQ=60)
                                                                        GCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCG                      <  SRR4302533.5313438/94‑1 (MQ=60)
                                                                         CATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGT                     >  SRR4302533.6233237/1‑94 (MQ=60)
                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                    >  SRR4302533.1569/1‑94 (MQ=60)
                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                    >  SRR4302533.3937154/1‑94 (MQ=60)
                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                    >  SRR4302533.4010710/1‑94 (MQ=60)
                                                                          ATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTT                    <  SRR4302533.3687376/94‑1 (MQ=60)
                                                                           TGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTA                   <  SRR4302533.3268199/94‑1 (MQ=60)
                                                                           TGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTA                   >  SRR4302533.5364800/1‑94 (MQ=60)
                                                                            GAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTAC                  >  SRR4302533.2382943/1‑94 (MQ=60)
                                                                               CAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGA               <  SRR4302533.1295340/94‑1 (MQ=60)
                                                                                AATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAA              >  SRR4302533.5009047/1‑94 (MQ=60)
                                                                                AATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAA              >  SRR4302533.3104376/1‑94 (MQ=60)
                                                                                   TACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAA           <  SRR4302533.5210123/94‑1 (MQ=60)
                                                                                   TACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAA           >  SRR4302533.965366/1‑94 (MQ=60)
                                                                                   TACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAA           <  SRR4302533.5958371/94‑1 (MQ=60)
                                                                                   TACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAA           <  SRR4302533.5958372/94‑1 (MQ=60)
                                                                                    ACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAAT          <  SRR4302533.4766053/94‑1 (MQ=60)
                                                                                    ACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAAT          <  SRR4302533.3876988/94‑1 (MQ=60)
                                                                                    ACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAAT          <  SRR4302533.3479308/94‑1 (MQ=60)
                                                                                     CGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATA         <  SRR4302533.3001641/94‑1 (MQ=60)
                                                                                       TGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATT       >  SRR4302533.1423115/1‑94 (MQ=60)
                                                                                       TGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATT       <  SRR4302533.964162/94‑1 (MQ=60)
                                                                                       TGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATT       <  SRR4302533.342487/94‑1 (MQ=60)
                                                                                        GAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTC      <  SRR4302533.5265725/94‑1 (MQ=60)
                                                                                        GAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTC      >  SRR4302533.5752572/1‑94 (MQ=60)
                                                                                         AAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT     >  SRR4302533.2760463/1‑94 (MQ=60)
                                                                                          AAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTG    <  SRR4302533.1846376/94‑1 (MQ=60)
                                                                                          AAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTG    <  SRR4302533.229981/94‑1 (MQ=60)
                                                                                          AAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTG    >  SRR4302533.265221/1‑94 (MQ=60)
                                                                                          AAAGGGGATGCAAAAAGATTACCTGGCGCTGGCGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTG    >  SRR4302533.6055587/1‑94 (MQ=60)
                                                                                           AAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC   <  SRR4302533.1411500/94‑1 (MQ=60)
                                                                                           AAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC   >  SRR4302533.1167426/1‑94 (MQ=60)
                                                                                           AAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC   >  SRR4302533.5793015/1‑94 (MQ=60)
                                                                                           AAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC   >  SRR4302533.4846076/1‑94 (MQ=60)
                                                                                            AGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  <  SRR4302533.1415372/94‑1 (MQ=60)
                                                                                            AGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  SRR4302533.2241873/1‑94 (MQ=60)
                                                                                            AGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  SRR4302533.4681712/1‑94 (MQ=60)
                                                                                            AGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  <  SRR4302533.3718713/94‑1 (MQ=60)
                                                                                             |                                                                                            
GGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGCA  >  NC_000913/1144579‑1144764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑