Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,148,033 A→G T18A (ACA→GCA)  fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,148,0330AG91.7% 677.3 / 40.3 252T18A (ACA→GCA) fabH3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base A (13/8);  new base G (134/97);  total (147/105)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

Only 100 of 258 total aligned reads displayed.

TGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC  >  NC_000913/1147942‑1148120
                                                                                           |                                                                                       
 gCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCa                                                                                       >  2:1317388/1‑93 (MQ=255)
  cAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaa                                                                                      <  2:4675945/93‑1 (MQ=255)
   aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                                                                                     <  1:1234278/93‑1 (MQ=255)
   aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                                                                                     <  2:4549537/93‑1 (MQ=255)
   aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACaaa                                                                                     <  2:8563574/93‑1 (MQ=255)
    aGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAc                                                                                    <  1:1138048/93‑1 (MQ=255)
      tGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGc                                                                                  >  1:4024983/1‑93 (MQ=255)
       gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                                                                                 >  1:1000443/1‑93 (MQ=255)
        aCGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCg                                                                                >  1:9191937/1‑93 (MQ=255)
          ggTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAt                                                                              >  1:6826747/1‑93 (MQ=255)
          ggTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAt                                                                              >  1:5577585/1‑93 (MQ=255)
           gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAANAAGTGCGGGCAAANGCCGAtt                                                                             >  1:7061131/1‑93 (MQ=255)
           gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAtt                                                                             >  2:7772915/1‑93 (MQ=255)
           gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAtt                                                                             <  2:4401825/93‑1 (MQ=255)
             atataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGCTTTg                                                                           >  1:3062130/1‑93 (MQ=255)
             atataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTg                                                                           <  1:1614099/93‑1 (MQ=255)
              tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                                                                          >  2:2492434/1‑93 (MQ=255)
              tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                                                                          >  2:8302029/1‑93 (MQ=255)
              tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                                                                          >  1:3594501/1‑93 (MQ=255)
              tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                                                                          >  1:4173968/1‑93 (MQ=255)
               ataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGa                                                                         >  2:3273525/1‑93 (MQ=255)
               ataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGa                                                                         <  1:1362110/93‑1 (MQ=255)
                taACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaa                                                                        >  2:8675089/1‑93 (MQ=255)
                  aCCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaa                                                                      >  1:4747497/1‑93 (MQ=255)
                     gAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAACAt                                                                   >  2:3280058/1‑93 (MQ=255)
                     gAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAt                                                                   <  1:1825719/93‑1 (MQ=255)
                       aaaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtgg                                                                 <  2:149937/93‑1 (MQ=255)
                       aaaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtgg                                                                 <  1:9831484/93‑1 (MQ=255)
                        aaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggt                                                                <  1:969780/93‑1 (MQ=255)
                         aGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAATGCGGGCAAACGCCGATTTGGAAACAAtggtg                                                               >  1:84851/1‑93 (MQ=255)
                          gTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggtgg                                                              >  1:6652106/1‑93 (MQ=255)
                           tGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGa                                                             <  1:7237869/93‑1 (MQ=255)
                            gACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGac                                                            <  2:5944454/93‑1 (MQ=255)
                             accgAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGaca                                                           <  1:4964106/90‑1 (MQ=255)
                              ccgAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGacac                                                          >  2:8165836/3‑93 (MQ=255)
                               cgAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACAcc                                                         <  2:2743568/92‑1 (MQ=255)
                                gAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACct                                                        <  1:4946342/93‑1 (MQ=255)
                                    gTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGa                                                    >  2:9429360/1‑93 (MQ=255)
                                    gTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGa                                                    >  2:298507/1‑93 (MQ=255)
                                    gTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGa                                                    >  2:3753311/1‑93 (MQ=255)
                                     tACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGAACACCTCTGAc                                                   >  1:5553923/1‑93 (MQ=255)
                                     tACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAGGGTGGACACCTCTGAc                                                   <  1:8884476/93‑1 (MQ=255)
                                       cATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGa                                                 <  2:1634445/93‑1 (MQ=255)
                                        aTGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAg                                                >  2:3980105/1‑93 (MQ=255)
                                         tGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAgg                                               >  2:8079715/1‑92 (MQ=255)
                                         tGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAgg                                               >  2:7381777/1‑92 (MQ=255)
                                         tGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                               >  1:8205422/1‑93 (MQ=255)
                                         tGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                               <  1:9095579/93‑1 (MQ=255)
                                         tGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                               >  1:9059415/1‑93 (MQ=255)
                                             taCGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                           <  2:6821868/93‑1 (MQ=255)
                                             taCGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                           <  1:10026976/93‑1 (MQ=255)
                                             taCGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                           >  2:5087070/1‑93 (MQ=255)
                                             taCGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                           <  1:7457251/93‑1 (MQ=255)
                                              aCGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAtt                                          >  1:3386280/1‑93 (MQ=255)
                                               cGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTg                                         >  2:9059259/1‑93 (MQ=255)
                                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGGGTGGATTGt                                        >  1:4295192/1‑93 (MQ=255)
                                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGt                                        <  1:9006643/93‑1 (MQ=255)
                                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGt                                        >  1:3788172/1‑93 (MQ=255)
                                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGt                                        >  1:2168176/1‑93 (MQ=255)
                                                 aaGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTc                                       >  1:7013213/1‑93 (MQ=255)
                                                  aGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCa                                      >  1:309493/1‑93 (MQ=255)
                                                   gATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAc                                     >  1:5102930/1‑93 (MQ=255)
                                                   gATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAc                                     >  2:4920472/1‑93 (MQ=255)
                                                    attattGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACt                                    <  1:3266195/93‑1 (MQ=255)
                                                      tattGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCg                                  >  2:242577/1‑93 (MQ=255)
                                                       attGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTTGACACCTCTGACGAGTGGATTGTCACTCGt                                 <  1:2932757/93‑1 (MQ=255)
                                                       attGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGt                                 >  2:2890571/1‑93 (MQ=255)
                                                        ttGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGtc                                >  1:8706637/1‑92 (MQ=255)
                                                        ttGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTa                                >  1:4773925/1‑93 (MQ=255)
                                                         tGGTACTGGCAGCTATCGGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTAc                               <  2:1093574/93‑1 (MQ=255)
                                                              cTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTa                          <  1:2735086/93‑1 (MQ=255)
                                                               tGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAt                         >  1:6034840/1‑93 (MQ=255)
                                                               tGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAt                         >  2:4904363/1‑93 (MQ=255)
                                                               tGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAt                         <  1:9332752/93‑1 (MQ=255)
                                                               tGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAt                         >  1:1997775/1‑93 (MQ=255)
                                                                ggCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATc                        >  1:4129554/1‑93 (MQ=255)
                                                                  cAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCcg                      <  1:7298467/93‑1 (MQ=255)
                                                                   aGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCcgc                     >  1:6032877/1‑93 (MQ=255)
                                                                    gCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCcgcg                    >  2:7201742/1‑93 (MQ=255)
                                                                      tATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGaa                  >  1:2881813/1‑93 (MQ=255)
                                                                       aTCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAAc                 <  2:2296095/93‑1 (MQ=255)
                                                                        tCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACg                >  1:4865324/1‑93 (MQ=255)
                                                                        tCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGCGGATTGTCACTCGCACCGGTATCCGCGAACg                >  1:5318324/1‑93 (MQ=255)
                                                                           gCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCca             >  2:1845968/1‑93 (MQ=255)
                                                                           gCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCca             <  1:9884425/93‑1 (MQ=255)
                                                                            cccGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCcac            >  1:2382702/1‑93 (MQ=255)
                                                                              cGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAt          <  1:4565851/93‑1 (MQ=255)
                                                                              cGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAt          >  2:5546658/1‑93 (MQ=255)
                                                                              cGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAt          <  2:430344/93‑1 (MQ=255)
                                                                               gAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAtt         <  2:3666268/93‑1 (MQ=255)
                                                                                aaCAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTg        <  2:9859926/93‑1 (MQ=255)
                                                                                  cAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGGATCCGCGAACGCCACACTGcc      >  2:7720202/1‑93 (MQ=255)
                                                                                   aaGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGGCACTCGTACCGGTACACGCGAACGCCAGATTggtc     >  1:841840/1‑90 (MQ=255)
                                                                                    aGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCcgc    >  2:6237597/1‑93 (MQ=255)
                                                                                    aGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCcgc    >  2:5603929/1‑93 (MQ=255)
                                                                                      tGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCgcgc  >  1:3213339/1‑93 (MQ=255)
                                                                                      tGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCgcgc  >  2:2070486/1‑93 (MQ=255)
                                                                                      tGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCgcgc  >  1:6469486/1‑93 (MQ=255)
                                                                                      tGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCgcgc  >  1:79938/1‑93 (MQ=255)
                                                                                      tGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCgcgc  >  1:2630567/1‑93 (MQ=255)
                                                                                           |                                                                                       
TGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC  >  NC_000913/1147942‑1148120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  >  NC_000913/1147940‑1148126
                                                                                             |                                                                                             
ATTGCAAGGGACGGTAGATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGG                                                                                               <  SRR4302540.3190220/94‑1 (MQ=60)
    CAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAA                                                                                           <  SRR4302540.1122274/94‑1 (MQ=60)
    CAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAA                                                                                           <  SRR4302540.2818650/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.2014847/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.3391331/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.1246884/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.7236114/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.7537376/94‑1 (MQ=60)
     AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                                                                                          <  SRR4302540.7537377/94‑1 (MQ=60)
      AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                                                                                         <  SRR4302540.9226091/94‑1 (MQ=60)
      AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                                                                                         <  SRR4302540.1149961/94‑1 (MQ=60)
      AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                                                                                         >  SRR4302540.9102421/1‑94 (MQ=60)
       GTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGC                                                                                        >  SRR4302540.4068377/1‑94 (MQ=60)
        TGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCC                                                                                       >  SRR4302540.1010603/1‑94 (MQ=60)
         GACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCG                                                                                      >  SRR4302540.9302183/1‑94 (MQ=60)
           CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                                                                                    >  SRR4302540.6902334/1‑94 (MQ=60)
           CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                                                                                    >  SRR4302540.5638985/1‑94 (MQ=60)
           CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                                                                                    <  SRR4302540.5586507/94‑1 (MQ=60)
            GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAANAAGTGCGGGCAAANGCCGATT                                                                                   >  SRR4302540.7139664/1‑94 (MQ=60)
            GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATT                                                                                   >  SRR4302540.995508/1‑94 (MQ=60)
            GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATT                                                                                   >  SRR4302540.7021625/1‑94 (MQ=60)
             GTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTT                                                                                  >  SRR4302540.3939820/1‑94 (MQ=60)
              TATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGCTTTG                                                                                 >  SRR4302540.3095724/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACCAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.5882995/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.4514762/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.3633771/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.478436/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                <  SRR4302540.1631813/94‑1 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                <  SRR4302540.1631812/94‑1 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.4219257/1‑94 (MQ=60)
               ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                                                                                >  SRR4302540.714691/1‑94 (MQ=60)
                 ATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAA                                                                              <  SRR4302540.1376499/94‑1 (MQ=60)
                  TAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAA                                                                             <  SRR4302540.1910259/94‑1 (MQ=60)
                   AACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAA                                                                            >  SRR4302540.4798159/1‑94 (MQ=60)
                      CGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAT                                                                         <  SRR4302540.4858525/94‑1 (MQ=60)
                       GAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATG                                                                        <  SRR4302540.1845684/94‑1 (MQ=60)
                        AAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGG                                                                       <  SRR4302540.9128084/94‑1 (MQ=60)
                        AAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGG                                                                       >  SRR4302540.3422365/1‑94 (MQ=60)
                         AAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGT                                                                      <  SRR4302540.9946465/94‑1 (MQ=60)
                          AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG                                                                     >  SRR4302540.3766111/1‑94 (MQ=60)
                          AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG                                                                     >  SRR4302540.85748/1‑94 (MQ=60)
                          AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG                                                                     <  SRR4302540.979763/94‑1 (MQ=60)
                          AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTG                                                                     <  SRR4302540.1217597/94‑1 (MQ=60)
                          AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAATGCGGGCAAACGCCGATTTGGAAACAATGGTG                                                                     >  SRR4302540.85747/1‑94 (MQ=60)
                           AGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGG                                                                    >  SRR4302540.6725686/1‑94 (MQ=60)
                           AGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGG                                                                    >  SRR4302540.163298/1‑94 (MQ=60)
                             TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC                                                                  <  SRR4302540.7318856/94‑1 (MQ=60)
                             TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC                                                                  <  SRR4302540.7318596/94‑1 (MQ=60)
                             TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC                                                                  >  SRR4302540.1595160/1‑94 (MQ=60)
                              GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA                                                                 >  SRR4302540.2044884/1‑94 (MQ=60)
                              GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA                                                                 >  SRR4302540.41816/1‑94 (MQ=60)
                              GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA                                                                 >  SRR4302540.41817/1‑94 (MQ=60)
                               ACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACAC                                                                <  SRR4302540.5017794/94‑1 (MQ=60)
                                 CGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCT                                                              <  SRR4302540.6690991/94‑1 (MQ=60)
                                  GAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTC                                                             >  SRR4302540.7543185/1‑94 (MQ=60)
                                  GAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTC                                                             <  SRR4302540.4999743/94‑1 (MQ=60)
                                   AGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCT                                                            <  SRR4302540.3072283/94‑1 (MQ=60)
                                   AGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCT                                                            >  SRR4302540.8970148/1‑94 (MQ=60)
                                     CGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGA                                                          <  SRR4302540.9206511/94‑1 (MQ=60)
                                      GTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGAC                                                         >  SRR4302540.10139380/1‑94 (MQ=60)
                                      GTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGAC                                                         <  SRR4302540.3269312/94‑1 (MQ=60)
                                      GTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGAACACCTCTGAC                                                         >  SRR4302540.5615096/1‑94 (MQ=60)
                                       TACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAGGGTGGACACCTCTGACG                                                        <  SRR4302540.8987314/94‑1 (MQ=60)
                                          ATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGT                                                     >  SRR4302540.8300776/1‑94 (MQ=60)
                                          ATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGT                                                     >  SRR4302540.9168184/1‑94 (MQ=60)
                                           TGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTG                                                    <  SRR4302540.3145778/94‑1 (MQ=60)
                                           TGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTG                                                    <  SRR4302540.9204858/94‑1 (MQ=60)
                                            GTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGG                                                   <  SRR4302540.8912860/94‑1 (MQ=60)
                                              ATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAT                                                 <  SRR4302540.9193506/94‑1 (MQ=60)
                                               TACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATT                                                <  SRR4302540.6735572/94‑1 (MQ=60)
                                               TACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATT                                                <  SRR4302540.7540394/94‑1 (MQ=60)
                                               TACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATT                                                <  SRR4302540.10143642/94‑1 (MQ=60)
                                               TACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATT                                                >  SRR4302540.3423232/1‑94 (MQ=60)
                                                 CGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGGGTGGATTGT                                              >  SRR4302540.4341925/1‑94 (MQ=60)
                                                 CGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGT                                              >  SRR4302540.3829476/1‑94 (MQ=60)
                                                 CGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGT                                              >  SRR4302540.2191555/1‑94 (MQ=60)
                                                 CGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGT                                              >  SRR4302540.6456448/1‑94 (MQ=60)
                                                 CGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGAGGATTGT                                              >  SRR4302540.2922274/1‑94 (MQ=60)
                                                 CGAACATTATCGGTACTGGCAGATACCTGCCCGAACAAGTGCGGCCCAACGCCGATTTGGAAAAAATGGTGGACCCCTCTGACGCGTGGATTGT                                              <  SRR4302540.3985000/94‑1 (MQ=60)
                                                  GAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTC                                             >  SRR4302540.3172436/1‑94 (MQ=60)
                                                  GAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTC                                             >  SRR4302540.7091077/1‑94 (MQ=60)
                                                  GAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTC                                             <  SRR4302540.9114695/94‑1 (MQ=60)
                                                   AAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCA                                            >  SRR4302540.312562/1‑94 (MQ=60)
                                                    AGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAC                                           <  SRR4302540.7132023/94‑1 (MQ=60)
                                                    AGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAC                                           <  SRR4302540.9674682/94‑1 (MQ=60)
                                                    AGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAC                                           >  SRR4302540.5158163/1‑94 (MQ=60)
                                                     GATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACT                                          <  SRR4302540.9142032/94‑1 (MQ=60)
                                                     GATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACT                                          <  SRR4302540.5008276/94‑1 (MQ=60)
                                                      ATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTC                                         <  SRR4302540.3301833/94‑1 (MQ=60)
                                                       TTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGaagcgctagatcgg                                        >  SRR4302540.8536561/1‑80 (MQ=60)
                                                       TTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCG                                        >  SRR4302540.3855018/1‑94 (MQ=60)
                                                       TTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCG                                        <  SRR4302540.2739447/94‑1 (MQ=60)
                                                        TATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGT                                       >  SRR4302540.9520781/1‑94 (MQ=60)
                                                         ATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTTGACACCTCTGACGAGTGGATTGTCACTCGTA                                      <  SRR4302540.2964934/94‑1 (MQ=60)
                                                         ATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTC                                      >  SRR4302540.8807698/1‑94 (MQ=60)
                                                         ATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTA                                      >  SRR4302540.4824924/1‑94 (MQ=60)
                                                          TTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTAC                                     >  SRR4302540.8438157/1‑94 (MQ=60)
                                                           TGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACC                                    <  SRR4302540.2931846/94‑1 (MQ=60)
                                                           TGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACC                                    >  SRR4302540.4774381/1‑94 (MQ=60)
                                                            GGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCG                                   >  SRR4302540.3538843/1‑94 (MQ=60)
                                                             GTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGG                                  >  SRR4302540.5324327/1‑94 (MQ=60)
                                                               GCTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTA                                <  SRR4302540.3008395/94‑1 (MQ=60)
                                                               ACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTA                                <  SRR4302540.5151112/94‑1 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               <  SRR4302540.2764614/94‑1 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.6101823/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.6101822/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.2019418/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               <  SRR4302540.1298540/94‑1 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.4272104/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.2203462/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTAT                               >  SRR4302540.5008500/1‑94 (MQ=60)
                                                                CTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAATCGTACCCGTAT                               >  SRR4302540.2203463/1‑94 (MQ=60)
                                                                 TGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATC                              <  SRR4302540.9444028/94‑1 (MQ=60)
                                                                 TGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATC                              >  SRR4302540.3124544/1‑94 (MQ=60)
                                                                 TGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATC                              >  SRR4302540.4174196/1‑94 (MQ=60)
                                                                  GGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCC                             >  SRR4302540.4856114/1‑94 (MQ=60)
                                                                    CAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGC                           >  SRR4302540.6099835/1‑94 (MQ=60)
                                                                    CAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGC                           <  SRR4302540.7379926/94‑1 (MQ=60)
                                                                    CAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGC                           >  SRR4302540.481612/1‑94 (MQ=60)
                                                                     AGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCG                          >  SRR4302540.8731222/1‑94 (MQ=60)
                                                                     AGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCG                          >  SRR4302540.1042594/1‑94 (MQ=60)
                                                                     AGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCG                          <  SRR4302540.1257637/94‑1 (MQ=60)
                                                                     AGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCG                          <  SRR4302540.4529895/94‑1 (MQ=60)
                                                                     AGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCCCG                          >  SRR4302540.579673/1‑94 (MQ=60)
                                                                      GCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGA                         <  SRR4302540.26156/94‑1 (MQ=60)
                                                                       CTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAA                        >  SRR4302540.5426628/1‑94 (MQ=60)
                                                                       CTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAA                        >  SRR4302540.2913168/1‑94 (MQ=60)
                                                                        TATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAAC                       <  SRR4302540.8051616/94‑1 (MQ=60)
                                                                        TATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAAC                       <  SRR4302540.3561191/94‑1 (MQ=60)
                                                                        TATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAAC                       >  SRR4302540.4109844/1‑94 (MQ=60)
                                                                         ATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACG                      >  SRR4302540.4917506/1‑94 (MQ=60)
                                                                         ATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACG                      >  SRR4302540.5376557/1‑94 (MQ=60)
                                                                         ATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGCGGATTGTCACTCGCACCGGTATCCGCGAACG                      >  SRR4302540.5376558/1‑94 (MQ=60)
                                                                            TGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCA                   >  SRR4302540.820786/1‑94 (MQ=60)
                                                                             GCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCAC                  >  SRR4302540.2408374/1‑94 (MQ=60)
                                                                             GCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCAC                  <  SRR4302540.9999840/94‑1 (MQ=60)
                                                                              CCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACA                 >  SRR4302540.6596026/1‑94 (MQ=60)
                                                                              CCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACA                 <  SRR4302540.4934206/94‑1 (MQ=60)
                                                                               CCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAT                >  SRR4302540.5628207/1‑94 (MQ=60)
                                                                               CCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAT                <  SRR4302540.9895022/94‑1 (MQ=60)
                                                                                CGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATT               >  SRR4302540.9870523/1‑94 (MQ=60)
                                                                                CGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATT               <  SRR4302540.4615155/94‑1 (MQ=60)
                                                                                   ACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCC            <  SRR4302540.9322264/94‑1 (MQ=60)
                                                                                    CAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGGCACTCGTACCGGTACACGCGAACGCCAgattggtc           >  SRR4302540.850770/1‑86 (MQ=60)
                                                                                    CAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACATCTGACGAGTGGATTGTCACTCGTACCGGTATCCGAGAACGCCACATTGCCG           >  SRR4302540.850769/1‑94 (MQ=60)
                                                                                      AGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCG         >  SRR4302540.6964821/1‑94 (MQ=60)
                                                                                      AGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCC         >  SRR4302540.6267703/1‑94 (MQ=60)
                                                                                       GTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC        >  SRR4302540.6541471/1‑94 (MQ=60)
                                                                                       GTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC        >  SRR4302540.3248225/1‑94 (MQ=60)
                                                                                       GTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC        >  SRR4302540.80775/1‑94 (MQ=60)
                                                                                       GTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGC        >  SRR4302540.2659037/1‑94 (MQ=60)
                                                                                            GGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAAC   <  SRR4302540.7392953/94‑1 (MQ=60)
                                                                                            GGCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAAC   <  SRR4302540.9208903/94‑1 (MQ=60)
                                                                                             GCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTCCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  <  SRR4302540.6045967/94‑1 (MQ=60)
                                                                                             GCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  >  SRR4302540.8310917/1‑94 (MQ=60)
                                                                                             GCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  <  SRR4302540.7660153/94‑1 (MQ=60)
                                                                                             GCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  <  SRR4302540.5608872/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACG  >  NC_000913/1147940‑1148126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑