Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,170,138	A→G	I1017T (ATC→ACC)	mfd ←	transcription‑repair coupling mutation frequency decline

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,170,138	0	A	G	100.0%	717.6 / NA	220	I1017T (ATC→ACC)	mfd	transcription‑repair coupling mutation frequency decline
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (102/118); total (102/118)

Only 100 of 235 total aligned reads displayed.

ATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCT  >  NC_000913/1170048‑1170227

atAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTg                                                                                         >  1:9192386/1‑93 (MQ=255)

 tAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGa                                                                                        <  2:1453176/93‑1 (MQ=255)

  aaGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGaa                                                                                       >  1:1549017/1‑93 (MQ=255)

      tCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAatca                                                                                   >  1:5405609/1‑93 (MQ=255)

      tCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTGGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAatca                                                                                   <  1:7981030/93‑1 (MQ=255)

       cGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAatcat                                                                                  <  2:8395056/93‑1 (MQ=255)

       cGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAatcat                                                                                  <  2:6280120/93‑1 (MQ=255)

         aCTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCt                                                                                <  2:8900873/93‑1 (MQ=255)

          cTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTg                                                                               >  1:6364841/1‑93 (MQ=255)

          cTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTg                                                                               <  2:9412528/93‑1 (MQ=255)

            ttGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGc                                                                             >  1:9244510/1‑93 (MQ=255)

            ttGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGc                                                                             >  2:8141193/1‑93 (MQ=255)

             tGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCa                                                                            >  1:8861484/1‑93 (MQ=255)

             tGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCa                                                                            >  2:7597930/1‑93 (MQ=255)

              gATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCaa                                                                           >  2:1071429/1‑93 (MQ=255)

              gATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCaa                                                                           >  2:6764255/1‑93 (MQ=255)

               aTCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAAt                                                                          <  2:1823866/93‑1 (MQ=255)

               aTCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAAt                                                                          <  2:1612618/93‑1 (MQ=255)

                 ctctTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAg                                                                        >  1:10008496/1‑93 (MQ=255)

                 ctctTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAg                                                                        <  2:3078110/93‑1 (MQ=255)

                 ctctTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAg                                                                        >  1:1881076/1‑93 (MQ=255)

                 ctctTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAg                                                                        <  1:338308/93‑1 (MQ=255)

                  tctTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGAGAAATCATCTGGCAATAgg                                                                       >  1:4500987/1‑92 (MQ=255)

                    ttCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGa                                                                     <  2:2222902/93‑1 (MQ=255)

                    ttCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGa                                                                     >  2:4291840/1‑93 (MQ=255)

                      ccAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACg                                                                   >  1:2732622/1‑93 (MQ=255)

                      ccAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACg                                                                   >  2:4184722/1‑93 (MQ=255)

                       cAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACgg                                                                  >  1:4444672/1‑93 (MQ=255)

                        aGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGc                                                                 >  1:1370204/1‑93 (MQ=255)

                         gTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCGTCTGGCAATAGCGACGGCa                                                                <  2:3493158/93‑1 (MQ=255)

                         gTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCa                                                                >  1:9710410/1‑93 (MQ=255)

                          ttCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCac                                                               >  1:2076552/1‑92 (MQ=255)

                          ttCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCAt                                                               >  2:7783314/1‑93 (MQ=255)

                           tCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATc                                                              <  2:4019833/93‑1 (MQ=255)

                           tCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATc                                                              <  2:3737474/93‑1 (MQ=255)

                           tCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATc                                                              <  2:6386539/93‑1 (MQ=255)

                           tCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATc                                                              <  1:6130426/93‑1 (MQ=255)

                           tCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTGCACGTCAGGGGTGAAAGCATTTGTCAATAGCGACGGCATc                                                              <  1:6883515/93‑1 (MQ=255)

                            cGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATcc                                                             <  1:5831953/93‑1 (MQ=255)

                             gTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCg                                                            <  1:4831721/93‑1 (MQ=255)

                               tttCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCa                                                          >  1:4326423/1‑93 (MQ=255)

                                ttCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAg                                                         <  1:2663615/93‑1 (MQ=255)

                                  ccGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCt                                                       <  1:3797694/93‑1 (MQ=255)

                                   cGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTc                                                      <  2:2116316/93‑1 (MQ=255)

                                     ttttGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGa                                                    >  2:3290464/1‑93 (MQ=255)

                                      ttgggCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGAc                                                   <  2:9113754/90‑1 (MQ=255)

                                        tGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACtt                                                 >  1:2765285/1‑93 (MQ=255)

                                         ggCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTc                                                <  1:6998206/93‑1 (MQ=255)

                                         ggCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTc                                                <  2:2744298/93‑1 (MQ=255)

                                         ggCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTc                                                <  1:2956151/93‑1 (MQ=255)

                                         ggCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTc                                                >  2:6194417/1‑93 (MQ=255)

                                           cgcTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTg                                              >  1:1012304/1‑93 (MQ=255)

                                            gcTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGt                                             <  2:7390993/93‑1 (MQ=255)

                                            gcTGGCAATACGTTTGTAGAACAACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATGGCGACGGCATCCTCACCTCGACTTCTGt                                             <  2:9193299/93‑1 (MQ=255)

                                                gCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgc                                         >  2:1631666/1‑93 (MQ=255)

                                                gCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgc                                         <  2:8773727/93‑1 (MQ=255)

                                                 cAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgct                                        <  2:8482451/93‑1 (MQ=255)

                                                 cAATACGTTTGTAGAACGACAGACGCGCGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgct                                        <  2:3883589/93‑1 (MQ=255)

                                                  aaTACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctg                                       >  1:8707433/1‑93 (MQ=255)

                                                  aaTACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTACGTTTgctg                                       >  2:7753521/1‑93 (MQ=255)

                                                   aTACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctgg                                      >  1:6233753/1‑93 (MQ=255)

                                                    tACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggc                                     <  1:6637680/93‑1 (MQ=255)

                                                    tACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggc                                     >  1:6945241/1‑93 (MQ=255)

                                                    tACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggc                                     >  1:7327370/1‑93 (MQ=255)

                                                    tACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGGGTAATCATCTGGCAATATCGACGGCAGCCGCATCTCCACTTCTGTTTtctggc                                     >  2:867617/1‑93 (MQ=255)

                                                     aCGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGGGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggct                                    >  1:6574485/1‑93 (MQ=255)

                                                      cGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggctg                                   <  2:9035895/93‑1 (MQ=255)

                                                      cGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTgctggctg                                   >  1:9467754/1‑93 (MQ=255)

                                                          tGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTgg                               >  2:2448647/1‑92 (MQ=255)

                                                          tGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTga                               <  2:6692002/93‑1 (MQ=255)

                                                           gTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTgag                              >  1:45592/1‑93 (MQ=255)

                                                            tAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTgaga                             <  1:2971469/93‑1 (MQ=255)

                                                              gAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATc                           <  2:3927219/93‑1 (MQ=255)

                                                              gAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATc                           <  1:9894935/93‑1 (MQ=255)

                                                               aaCGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTACTGTTTGCTGGCTGGTGAGATCt                          >  2:5534913/1‑93 (MQ=255)

                                                                aCGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCtt                         >  1:4265428/1‑93 (MQ=255)

                                                                 cGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTc                        <  2:7317262/93‑1 (MQ=255)

                                                                   aCAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCa                      >  1:352890/1‑93 (MQ=255)

                                                                    cAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAg                     <  2:10008495/93‑1 (MQ=255)

                                                                    cAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAg                     >  1:2516598/1‑93 (MQ=255)

                                                                      gACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGca                   >  1:6027673/1‑92 (MQ=255)

                                                                      gACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCg                   >  1:9150778/1‑93 (MQ=255)

                                                                       aCGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGa                  <  1:1638092/93‑1 (MQ=255)

                                                                       aCGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGa                  >  1:7148173/1‑93 (MQ=255)

                                                                        cgcgTGTTCACGTCAGGGGCGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGag                 >  1:2238301/1‑92 (MQ=255)

                                                                           gtgtTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGc              >  2:7020690/1‑93 (MQ=255)

                                                                           gtgtTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGc              <  2:467522/93‑1 (MQ=255)

                                                                            tgtTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCt             <  2:2657213/93‑1 (MQ=255)

                                                                             gtTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTc            <  2:8023773/93‑1 (MQ=255)

                                                                             gtTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTc            <  2:9522461/93‑1 (MQ=255)

                                                                                cACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTcgcg         >  2:8183244/1‑93 (MQ=255)

                                                                                cACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTcgcg         <  1:6477092/93‑1 (MQ=255)

                                                                                   ggcAGGGGTGAACTCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGccc      <  2:5366412/91‑1 (MQ=255)

                                                                                   gTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTcc      <  2:5124267/93‑1 (MQ=255)

                                                                                   gTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTcc      <  1:8183167/93‑1 (MQ=255)

                                                                                    tCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCg     <  2:4428944/93‑1 (MQ=255)

                                                                                    tCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCg     >  2:5562611/1‑93 (MQ=255)

                                                                                      aGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGc   >  2:8353256/1‑93 (MQ=255)

                                                                                      aGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGCCCGGc   >  1:568999/1‑93 (MQ=255)

                                                                                       ggggTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGGCCCGCt  >  1:2718644/1‑93 (MQ=255)

ATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCT  >  NC_000913/1170048‑1170227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCA  >  NC_000913/1170045‑1170231

TCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGG                                                                                               <  SRR4302540.4531481/94‑1 (MQ=60)

TCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGG                                                                                               >  SRR4302540.1872266/1‑94 (MQ=60)

TCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGG                                                                                               <  SRR4302540.7631541/94‑1 (MQ=60)

  GATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTG                                                                                             >  SRR4302540.9302638/1‑94 (MQ=60)

    TAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAA                                                                                           >  SRR4302540.1566098/1‑94 (MQ=60)

      AGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAAT                                                                                         >  SRR4302540.4349120/1‑94 (MQ=60)

       GCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGCAATC                                                                                        >  SRR4302540.10053620/1‑94 (MQ=60)

       GCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATC                                                                                        >  SRR4302540.10053619/1‑94 (MQ=60)

        CTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCA                                                                                       >  SRR4302540.4575279/1‑94 (MQ=60)

        CTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCA                                                                                       >  SRR4302540.5465042/1‑94 (MQ=60)

        CTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCA                                                                                       <  SRR4302540.2725838/94‑1 (MQ=60)

         TCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTGGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCAT                                                                                      <  SRR4302540.8073987/94‑1 (MQ=60)

          tgtCTTTGATCTCTTCCAGTTCGTTTGCCGTTGTGGCTCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATC                                                                                     <  SRR4302540.6406686/91‑1 (MQ=60)

           GACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCT                                                                                    <  SRR4302540.2473338/94‑1 (MQ=60)

           GACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCT                                                                                    <  SRR4302540.8764135/94‑1 (MQ=60)

           GACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATAATCT                                                                                    >  SRR4302540.6277575/1‑94 (MQ=60)

            ACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTG                                                                                   >  SRR4302540.6435708/1‑94 (MQ=60)

             CTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGG                                                                                  >  SRR4302540.7027084/1‑94 (MQ=60)

              TTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGC                                                                                 >  SRR4302540.9355212/1‑94 (MQ=60)

               TTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCA                                                                                >  SRR4302540.8964027/1‑94 (MQ=60)

               TTGATCTCTTCCAGTTCGTTTTCCGTTTGGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCA                                                                                <  SRR4302540.5036287/94‑1 (MQ=60)

                 GATCTCTTCCAGTTCGTTGTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAAT                                                                              <  SRR4302540.9393169/94‑1 (MQ=60)

                  ATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATA                                                                             <  SRR4302540.7135603/94‑1 (MQ=60)

                  ATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATA                                                                             <  SRR4302540.7300563/94‑1 (MQ=60)

                  ATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATA                                                                             <  SRR4302540.6129312/94‑1 (MQ=60)

                  ATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATA                                                                             >  SRR4302540.9184090/1‑94 (MQ=60)

                   TCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAG                                                                            >  SRR4302540.1901676/1‑94 (MQ=60)

                   TCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAG                                                                            >  SRR4302540.10125048/1‑94 (MQ=60)

                   TCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAG                                                                            <  SRR4302540.10155422/94‑1 (MQ=60)

                    CTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGC                                                                           <  SRR4302540.341720/94‑1 (MQ=60)

                    CTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGC                                                                           <  SRR4302540.6642872/94‑1 (MQ=60)

                    CTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGC                                                                           <  SRR4302540.6092211/94‑1 (MQ=60)

                    CTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGGGAAATCATCTGGCAATAGG                                                                           >  SRR4302540.3012173/1‑94 (MQ=60)

                    CTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGAGAAATCATCTGGCAATAGG                                                                           >  SRR4302540.4549778/1‑94 (MQ=60)

                      CTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGA                                                                         <  SRR4302540.6963588/94‑1 (MQ=60)

                       TTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGAC                                                                        <  SRR4302540.4131425/94‑1 (MQ=60)

                        TCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATGTGGCAATAGCGACG                                                                       >  SRR4302540.5544905/1‑94 (MQ=60)

                        TCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACG                                                                       >  SRR4302540.2762113/1‑94 (MQ=60)

                        TCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGAAAGACGCGTGTTCACGGCAGGGGTGACATCATCTGGCCATAGCAACG                                                                       >  SRR4302540.2762111/1‑94 (MQ=60)

                         CCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGG                                                                      >  SRR4302540.4492840/1‑94 (MQ=60)

                         CCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCAGCTGGCAATAGCGACGG                                                                      >  SRR4302540.9075443/1‑94 (MQ=60)

                          CAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGC                                                                     >  SRR4302540.1384724/1‑94 (MQ=60)

                           AGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCA                                                                    >  SRR4302540.9824534/1‑94 (MQ=60)

                            GTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCAC                                                                   >  SRR4302540.2098871/1‑94 (MQ=60)

                              TCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCC                                                                 >  SRR4302540.2996992/1‑94 (MQ=60)

                              TCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCC                                                                 <  SRR4302540.6198708/94‑1 (MQ=60)

                              TCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTGCACGTCAGGGGTGAAAGCATTTGTCAATAGCGACGGCATCC                                                                 <  SRR4302540.6959823/94‑1 (MQ=60)

                               CGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCG                                                                <  SRR4302540.5896436/94‑1 (MQ=60)

                               CGTTTTCCGTTTTGGCGCTGGCAATACCTTTGTAGAACGACAGACCCGTGTTCACGTCAGGGGTGAAATGATCTGGCAATAGAGACGGCATCCG                                                                >  SRR4302540.9907238/1‑94 (MQ=60)

                                GTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGC                                                               <  SRR4302540.4883480/94‑1 (MQ=60)

                                GTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGC                                                               >  SRR4302540.4827837/1‑94 (MQ=60)

                                 TTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCA                                                              <  SRR4302540.7470398/94‑1 (MQ=60)

                                 TTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCA                                                              >  SRR4302540.4032291/1‑94 (MQ=60)

                                 TTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCA                                                              >  SRR4302540.4373524/1‑94 (MQ=60)

                                   TTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGC                                                            <  SRR4302540.2692503/94‑1 (MQ=60)

                                     CCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTC                                                          <  SRR4302540.3839075/94‑1 (MQ=60)

                                       GTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGC                                                        >  SRR4302540.2003937/1‑94 (MQ=60)

                                        TTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGAC                                                       <  SRR4302540.1930598/94‑1 (MQ=60)

                                          TTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTT                                                     >  SRR4302540.2795163/1‑94 (MQ=60)

                                          TTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTT                                                     <  SRR4302540.2776660/94‑1 (MQ=60)

                                            GGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCT                                                   <  SRR4302540.2988711/94‑1 (MQ=60)

                                            GGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCT                                                   <  SRR4302540.7075874/94‑1 (MQ=60)

                                             GCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTG                                                  >  SRR4302540.1022565/1‑94 (MQ=60)

                                               GCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTT                                                >  SRR4302540.7016455/1‑94 (MQ=60)

                                               GCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTT                                                <  SRR4302540.4507189/94‑1 (MQ=60)

                                                 TGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTG                                              >  SRR4302540.5834318/1‑94 (MQ=60)

                                                 TGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGGGAAATCATCTGGGAATGGCGACGGCATCCGAAGCTCGACTTCTtgtgg                                              >  SRR4302540.5834320/1‑89 (MQ=60)

                                                    CAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTG                                           >  SRR4302540.8808506/1‑94 (MQ=60)

                                                     AATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGG                                          >  SRR4302540.6303081/1‑94 (MQ=60)

                                                      ATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGC                                         >  SRR4302540.7409183/1‑94 (MQ=60)

                                                      ATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGC                                         >  SRR4302540.7022541/1‑94 (MQ=60)

                                                       TACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCT                                        <  SRR4302540.6711134/94‑1 (MQ=60)

                                                       TACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGGGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCT                                        >  SRR4302540.6647316/1‑94 (MQ=60)

                                                        ACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTG                                       >  SRR4302540.4796187/1‑94 (MQ=60)

                                                        ACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTG                                       >  SRR4302540.9580067/1‑94 (MQ=60)

                                                           TTTGTAGAACGACAGACGCGCGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTG                                    >  SRR4302540.6936662/1‑94 (MQ=60)

                                                            TTGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGA                                   >  SRR4302540.3120750/1‑94 (MQ=60)

                                                             TGTAGAGCGACAGACGCGTGTGCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAG                                  <  SRR4302540.2944497/94‑1 (MQ=60)

                                                             TGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAG                                  >  SRR4302540.46059/1‑94 (MQ=60)

                                                             TGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAG                                  >  SRR4302540.2604806/1‑94 (MQ=60)

                                                             TGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAG                                  <  SRR4302540.6691429/94‑1 (MQ=60)

                                                             TGTAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAG                                  >  SRR4302540.4419919/1‑94 (MQ=60)

                                                               TAGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGAT                                <  SRR4302540.3004287/94‑1 (MQ=60)

                                                                AGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGGGAGATT                               >  SRR4302540.1856624/1‑94 (MQ=60)

                                                                AGAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTAGTGAGATC                               <  SRR4302540.8937967/94‑1 (MQ=60)

                                                                 GAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCT                              <  SRR4302540.6389478/94‑1 (MQ=60)

                                                                 GAACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCT                              <  SRR4302540.10010486/94‑1 (MQ=60)

                                                                  AACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTT                             <  SRR4302540.361734/94‑1 (MQ=60)

                                                                  AACGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTT                             >  SRR4302540.4311818/1‑94 (MQ=60)

                                                                    CGACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCC                           <  SRR4302540.5837699/94‑1 (MQ=60)

                                                                     GACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCA                          >  SRR4302540.356507/1‑94 (MQ=60)

                                                                     GACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCA                          <  SRR4302540.1098463/94‑1 (MQ=60)

                                                                     GACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCA                          <  SRR4302540.437520/94‑1 (MQ=60)

                                                                      ACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAG                         >  SRR4302540.2543781/1‑94 (MQ=60)

                                                                      ACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAG                         >  SRR4302540.2543782/1‑94 (MQ=60)

                                                                      ACAGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAG                         <  SRR4302540.3442586/94‑1 (MQ=60)

                                                                        AGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCG                       >  SRR4302540.9260657/1‑94 (MQ=60)

                                                                        AGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCG                       <  SRR4302540.163969/94‑1 (MQ=60)

                                                                        AGACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCA                       >  SRR4302540.6094559/1‑94 (MQ=60)

                                                                         GACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGA                      >  SRR4302540.7227827/1‑94 (MQ=60)

                                                                          GCGCGTGTTCCCGTCAGGGCTGAATTCGTGTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGAC                     <  SRR4302540.9334004/94‑1 (MQ=60)

                                                                          ACGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGAC                     <  SRR4302540.1656005/94‑1 (MQ=60)

                                                                          ACGCGTGTTCACGTCAGGGGCGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGAG                     >  SRR4302540.2262370/1‑94 (MQ=60)

                                                                          ACGCGTGTTCACGTAAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGAC                     <  SRR4302540.939748/94‑1 (MQ=60)

                                                                           CGCGTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACG                    <  SRR4302540.9637773/94‑1 (MQ=60)

                                                                              GTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCT                 <  SRR4302540.6954024/94‑1 (MQ=60)

                                                                              GTGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCC                 >  SRR4302540.1440837/1‑94 (MQ=60)

                                                                               TGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGTCGGCTGGGGAGATCTTCCACCGCCGGCTC                >  SRR4302540.8259194/1‑94 (MQ=60)

                                                                               TGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTC                >  SRR4302540.8259193/1‑94 (MQ=60)

                                                                               TGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTC                <  SRR4302540.2789097/94‑1 (MQ=60)

                                                                               TGTTCACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTC                <  SRR4302540.1519466/94‑1 (MQ=60)

                                                                                   CACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGT            <  SRR4302540.6549153/94‑1 (MQ=60)

                                                                                   CACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGT            <  SRR4302540.6549155/94‑1 (MQ=60)

                                                                                   CACGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGT            <  SRR4302540.6549156/94‑1 (MQ=60)

                                                                                     CGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCC          <  SRR4302540.2118691/94‑1 (MQ=60)

                                                                                     CGTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCC          <  SRR4302540.6205375/94‑1 (MQ=60)

                                                                                      GTCAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCG         <  SRR4302540.8278217/94‑1 (MQ=60)

                                                                                        CAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGC       >  SRR4302540.7329117/1‑94 (MQ=60)

                                                                                        CAGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGCCCGGC       >  SRR4302540.575181/1‑94 (MQ=60)

                                                                                         AGGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGGCCCGCT      >  SRR4302540.2747955/1‑94 (MQ=60)

                                                                                          GGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTgccccccggctt     >  SRR4302540.8275605/1‑82 (MQ=60)

                                                                                          GGGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTT     >  SRR4302540.8275606/1‑94 (MQ=60)

                                                                                           GGGTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTT    >  SRR4302540.8317999/1‑94 (MQ=60)

                                                                                           GGGTGAAATCAGCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTT    <  SRR4302540.7821742/94‑1 (MQ=60)

                                                                                             GTGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCA  <  SRR4302540.8844427/94‑1 (MQ=60)

TCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGCGCTGGCAATACGTTTGTAGAACGACAGACGCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGCTGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCA  >  NC_000913/1170045‑1170231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`