Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,173,983	C→T	G136R (GGG→AGG)	ycfT ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,173,983	0	C	T	99.6%	850.7 / ‑5.8	231	G136R (GGG→AGG)	ycfT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (105/125); minor base G (0/1); total (105/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 238 total aligned reads displayed.

GCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCAC  >  NC_000913/1173893‑1174072

gCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGt                                                                                         <  2:2952943/93‑1 (MQ=255)

 cAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCtgtg                                                                                        >  2:5365650/1‑93 (MQ=255)

   gcgcCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCa                                                                                      <  2:1336106/93‑1 (MQ=255)

   gcgcCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCa                                                                                      <  1:1487452/93‑1 (MQ=255)

     gcCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAgg                                                                                    >  2:245012/1‑93 (MQ=255)

      ccAGACTGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATCCCACAATCTGGTGCTGGCGGTGATCATCCTGTGCAGGa                                                                                   <  2:7768277/93‑1 (MQ=255)

      ccAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGa                                                                                   <  1:1615900/93‑1 (MQ=255)

      ccAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGa                                                                                   <  2:4075104/93‑1 (MQ=255)

       cAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGaa                                                                                  >  2:9175652/1‑93 (MQ=255)

           cGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTTCTGGCGGTGATCATCCTGTGCAGGCACTCa                                                                              >  2:2173294/1‑93 (MQ=255)

           cGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCa                                                                              >  2:8386137/1‑93 (MQ=255)

           cGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCa                                                                              >  1:6357209/1‑93 (MQ=255)

            ggCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAc                                                                             >  1:305947/1‑93 (MQ=255)

             gcgaaaaaTTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAcc                                                                            <  1:2435318/90‑1 (MQ=255)

             gCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAcc                                                                            <  1:8906785/93‑1 (MQ=255)

               tAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCgg                                                                          >  2:9702918/1‑93 (MQ=255)

               tAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCgg                                                                          >  2:865286/1‑93 (MQ=255)

               tAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCgg                                                                          <  1:1419213/93‑1 (MQ=255)

                aaaaaTTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGt                                                                         >  2:9088575/1‑93 (MQ=255)

                   aaTTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGa                                                                      >  1:9448687/1‑93 (MQ=255)

                     ttttACATACCACGAAATAGGCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAAt                                                                    <  2:5557650/93‑1 (MQ=255)

                     ttttACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAAt                                                                    >  2:5074741/1‑93 (MQ=255)

                     atttACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAAt                                                                    <  1:6032785/92‑1 (MQ=255)

                      tttACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATc                                                                   <  1:1354440/93‑1 (MQ=255)

                        tACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCgg                                                                 >  1:6623355/1‑93 (MQ=255)

                        tACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCgg                                                                 >  2:1254371/1‑93 (MQ=255)

                         tcATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGc                                                                <  1:3161645/92‑1 (MQ=255)

                         aCATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGc                                                                <  2:754036/93‑1 (MQ=255)

                           aTACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAt                                                              <  1:7415499/93‑1 (MQ=255)

                           aTACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAt                                                              >  1:8329598/1‑93 (MQ=255)

                            tACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATa                                                             >  1:339721/1‑93 (MQ=255)

                            tACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATa                                                             >  1:4797785/1‑93 (MQ=255)

                            tACCACGAAATAGACAATTAACCCATACAGATACCACAAGCTGGGGCTGGCGGTGATCATCCTGTCCAGGAACTCACCGGTGGAATCGGCATa                                                             <  1:870844/93‑1 (MQ=255)

                             aCCGCGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATaa                                                            >  1:263429/1‑93 (MQ=255)

                             aCCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATaa                                                            <  1:7174895/93‑1 (MQ=255)

                              ccACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAg                                                           <  2:8100237/93‑1 (MQ=255)

                               cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAgg                                                          >  2:3417394/1‑92 (MQ=255)

                               cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGc                                                          <  1:1129318/93‑1 (MQ=255)

                               cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGc                                                          <  1:5924842/93‑1 (MQ=255)

                               cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACACACCGGTGGAATCGGCATAAGc                                                          <  2:5417649/93‑1 (MQ=255)

                                 cGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCgg                                                        <  2:6903267/93‑1 (MQ=255)

                                 cGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCgg                                                        <  1:495071/93‑1 (MQ=255)

                                   aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCa                                                      <  1:708250/93‑1 (MQ=255)

                                   aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCa                                                      >  1:3742551/1‑93 (MQ=255)

                                     aTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAtt                                                    <  1:2987049/93‑1 (MQ=255)

                                     aTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAtg                                                    >  1:2525399/1‑92 (MQ=255)

                                      tAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTg                                                   >  1:4431287/1‑93 (MQ=255)

                                      aaGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTg                                                   <  1:9889958/92‑1 (MQ=255)

                                        gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGa                                                 >  2:4396553/1‑93 (MQ=255)

                                        gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGa                                                 <  2:1768762/93‑1 (MQ=255)

                                        gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGAATTGGa                                                 >  2:4760006/1‑93 (MQ=255)

                                         aCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAg                                                <  2:7302534/93‑1 (MQ=255)

                                         aCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAg                                                <  2:4925233/93‑1 (MQ=255)

                                           aaTTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGc                                              <  1:7332016/93‑1 (MQ=255)

                                           aaTTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGc                                              >  1:7794745/1‑93 (MQ=255)

                                             ttAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAt                                            <  1:9745877/93‑1 (MQ=255)

                                             ttAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAt                                            <  2:1697989/93‑1 (MQ=255)

                                             ttAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAt                                            <  2:8313912/93‑1 (MQ=255)

                                              tAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAtt                                           <  2:3919989/93‑1 (MQ=255)

                                                 cGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCt                                        <  1:9176934/93‑1 (MQ=255)

                                                  gCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCtt                                       <  1:3479648/93‑1 (MQ=255)

                                                  gCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCtt                                       <  2:547552/93‑1 (MQ=255)

                                                   cATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTa                                      >  1:6072756/1‑93 (MQ=255)

                                                   cATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTa                                      <  2:3521896/93‑1 (MQ=255)

                                                   cATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTa                                      <  2:2706540/93‑1 (MQ=255)

                                                    aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTaa                                     >  1:5348318/1‑93 (MQ=255)

                                                    aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTaa                                     >  2:2205309/1‑93 (MQ=255)

                                                     tACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTaaa                                    >  1:1895979/1‑93 (MQ=255)

                                                      aCAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAAt                                   >  2:425867/1‑93 (MQ=255)

                                                       cAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATc                                  >  1:4600068/1‑93 (MQ=255)

                                                        aGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATcg                                 <  2:7232372/93‑1 (MQ=255)

                                                         gATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATcgc                                >  2:5994326/1‑93 (MQ=255)

                                                         gATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATcgc                                <  2:2045381/93‑1 (MQ=255)

                                                         gATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATcgc                                <  1:2348805/93‑1 (MQ=255)

                                                           tACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCgcgc                              <  2:9712384/93‑1 (MQ=255)

                                                           tACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCgcgc                              >  2:4368324/1‑93 (MQ=255)

                                                             ccACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTc                            >  1:10021132/1‑93 (MQ=255)

                                                                caAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAgg                         >  2:6315243/1‑93 (MQ=255)

                                                                caAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAgg                         <  2:6581392/93‑1 (MQ=255)

                                                                 aaGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGt                        <  2:6743239/93‑1 (MQ=255)

                                                                  aGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTg                       <  1:2041049/93‑1 (MQ=255)

                                                                    cTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgcc                     >  1:7612265/1‑93 (MQ=255)

                                                                      ggTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccag                   >  1:2755681/1‑93 (MQ=255)

                                                                        tGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccagcc                 <  2:3670821/93‑1 (MQ=255)

                                                                        tGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccagcc                 <  1:6852617/93‑1 (MQ=255)

                                                                         gCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccagcca                <  1:2832698/93‑1 (MQ=255)

                                                                             gCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTgg            <  1:5443928/93‑1 (MQ=255)

                                                                             gCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTgg            >  1:4835343/1‑93 (MQ=255)

                                                                              cGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAAGTGCCAGCCACTGGt           >  1:5496868/1‑93 (MQ=255)

                                                                                gTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTc         >  2:1556294/1‑93 (MQ=255)

                                                                                gTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTc         <  1:4219779/93‑1 (MQ=255)

                                                                                 tGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCa        >  1:5084154/1‑93 (MQ=255)

                                                                                  gATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAg       >  1:1268361/1‑93 (MQ=255)

                                                                                   atcatcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAgc      >  1:1942059/1‑93 (MQ=255)

                                                                                   agattcCTGTGCAGGAACTCACCGGTGGGATCGGCATAAGCGGCCTTGGAGGCATTGCTTAAATCGCGATAAGGGGCCAGCCAGTGTGTCAgc      >  2:3165244/5‑93 (MQ=255)

                                                                                     catcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAgcgc    >  2:9503652/1‑93 (MQ=255)

                                                                                      atcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCa   <  2:6254854/93‑1 (MQ=255)

                                                                                      atcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCa   >  1:8089223/1‑93 (MQ=255)

                                                                                       tcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCAc  >  1:853275/1‑93 (MQ=255)

                                                                                       tcCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCAc  >  2:2544204/1‑93 (MQ=255)

GCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCAC  >  NC_000913/1173893‑1174072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAA  >  NC_000913/1173890‑1174076

GTGGCAGCGCCAGACGGCTAAAAATTTTAAATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGGGATCATCCT                                                                                               >  SRR4302540.5801051/1‑94 (MQ=60)

 TGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTG                                                                                              <  SRR4302540.7717568/94‑1 (MQ=60)

  GGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGT                                                                                             <  SRR4302540.6900185/94‑1 (MQ=60)

    CAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGC                                                                                           <  SRR4302540.7163824/94‑1 (MQ=60)

     AGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCA                                                                                          >  SRR4302540.2619195/1‑94 (MQ=60)

     AGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCA                                                                                          >  SRR4302540.8418807/1‑94 (MQ=60)

      GCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAG                                                                                         <  SRR4302540.635738/94‑1 (MQ=60)

      GCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAG                                                                                         <  SRR4302540.1503795/94‑1 (MQ=60)

         CCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAA                                                                                      <  SRR4302540.1633625/94‑1 (MQ=60)

          CAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAAC                                                                                     >  SRR4302540.1144817/1‑94 (MQ=60)

          CAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAAC                                                                                     >  SRR4302540.9140748/1‑94 (MQ=60)

           AGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACT                                                                                    <  SRR4302540.1403316/94‑1 (MQ=60)

             ACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCA                                                                                  >  SRR4302540.6428003/1‑94 (MQ=60)

              TGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAC                                                                                 >  SRR4302540.308983/1‑94 (MQ=60)

              CGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAC                                                                                 >  SRR4302540.4477728/1‑94 (MQ=60)

              CGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCAC                                                                                 <  SRR4302540.9403620/94‑1 (MQ=60)

               GGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACC                                                                                <  SRR4302540.5950370/94‑1 (MQ=60)

               GGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACC                                                                                <  SRR4302540.1458508/94‑1 (MQ=60)

               GGATAAAAATTTTACATACCACGAAATAGACAATTAACGCATGCAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACC                                                                                <  SRR4302540.1458507/94‑1 (MQ=60)

                GCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCG                                                                               <  SRR4302540.9009916/94‑1 (MQ=60)

                GCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCG                                                                               <  SRR4302540.3044486/94‑1 (MQ=60)

                GCGAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCG                                                                               <  SRR4302540.2461551/94‑1 (MQ=60)

                 CTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGG                                                                              >  SRR4302540.1360075/1‑94 (MQ=60)

                  TAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGT                                                                             <  SRR4302540.1434559/94‑1 (MQ=60)

                  TAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGT                                                                             <  SRR4302540.2123415/94‑1 (MQ=60)

                     AAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGA                                                                          >  SRR4302540.9560850/1‑94 (MQ=60)

                     AAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGA                                                                          >  SRR4302540.8627136/1‑94 (MQ=60)

                        ATTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATC                                                                       <  SRR4302540.6099742/94‑1 (MQ=60)

                         acTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGATGATCATCCTGTGCAGCAACCCACCGGTGGAATCG                                                                      <  SRR4302540.1368699/92‑1 (MQ=60)

                         TTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCG                                                                      <  SRR4302540.1368700/94‑1 (MQ=60)

                          TTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGG                                                                     >  SRR4302540.6696686/1‑94 (MQ=60)

                           TACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGC                                                                    <  SRR4302540.3604002/94‑1 (MQ=60)

                            TCATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCA                                                                   <  SRR4302540.3196039/94‑1 (MQ=60)

                            ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCA                                                                   <  SRR4302540.7404464/94‑1 (MQ=60)

                             CATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAT                                                                  <  SRR4302540.6554700/94‑1 (MQ=60)

                             CATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAT                                                                  <  SRR4302540.1775491/94‑1 (MQ=60)

                             CATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAT                                                                  >  SRR4302540.6058129/1‑94 (MQ=60)

                             CATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCAT                                                                  >  SRR4302540.8426278/1‑94 (MQ=60)

                              ATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATA                                                                 >  SRR4302540.4849128/1‑94 (MQ=60)

                              ATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATA                                                                 >  SRR4302540.343150/1‑94 (MQ=60)

                              ATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATA                                                                 >  SRR4302540.8818530/1‑94 (MQ=60)

                              ATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATA                                                                 <  SRR4302540.7498094/94‑1 (MQ=60)

                               TACCGCGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAA                                                                >  SRR4302540.265998/1‑94 (MQ=60)

                               TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAA                                                                >  SRR4302540.6753802/1‑94 (MQ=60)

                               TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAA                                                                >  SRR4302540.265999/1‑94 (MQ=60)

                               TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAA                                                                <  SRR4302540.880089/94‑1 (MQ=60)

                               TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAA                                                                <  SRR4302540.7839206/94‑1 (MQ=60)

                               TACCACGAAATAGACAATTAACCCATACAGATACCACAAGCTGGGGCTGGCGGTGATCATCCTGTCCAGGAACTCACCGGTGGAATCGGCATAA                                                                <  SRR4302540.880090/94‑1 (MQ=60)

                                ACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAG                                                               <  SRR4302540.7254869/94‑1 (MQ=60)

                                 CCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGC                                                              <  SRR4302540.7860750/94‑1 (MQ=60)

                                  CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCG                                                             <  SRR4302540.7625193/94‑1 (MQ=60)

                                  CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCG                                                             <  SRR4302540.5990306/94‑1 (MQ=60)

                                  CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCG                                                             <  SRR4302540.1141068/94‑1 (MQ=60)

                                  CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCG                                                             <  SRR4302540.4744791/94‑1 (MQ=60)

                                    CGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGC                                                           <  SRR4302540.500380/94‑1 (MQ=60)

                                     GAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCA                                                          >  SRR4302540.3783390/1‑94 (MQ=60)

                                     GAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCA                                                          >  SRR4302540.6984883/1‑94 (MQ=60)

                                      AAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT                                                         >  SRR4302540.4529653/1‑94 (MQ=60)

                                      AAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT                                                         <  SRR4302540.715866/94‑1 (MQ=60)

                                       AATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATT                                                        <  SRR4302540.2057406/94‑1 (MQ=60)

                                       AATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATG                                                        >  SRR4302540.2552662/1‑94 (MQ=60)

                                        ATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTG                                                       >  SRR4302540.4861968/1‑94 (MQ=60)

                                        ATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTG                                                       >  SRR4302540.4479298/1‑94 (MQ=60)

                                        ATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTG                                                       >  SRR4302540.4479297/1‑94 (MQ=60)

                                        ATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTG                                                       <  SRR4302540.3020000/94‑1 (MQ=60)

                                         TAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGG                                                      <  SRR4302540.10005434/94‑1 (MQ=60)

                                         AAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGG                                                      <  SRR4302540.10005435/94‑1 (MQ=60)

                                          AGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGA                                                     <  SRR4302540.5272032/94‑1 (MQ=60)

                                            ACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGG                                                   >  SRR4302540.3430672/1‑94 (MQ=60)

                                             CAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGC                                                  >  SRR4302540.4992790/1‑94 (MQ=60)

                                             CAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGC                                                  >  SRR4302540.7885854/1‑94 (MQ=60)

                                             CAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGC                                                  <  SRR4302540.7270939/94‑1 (MQ=60)

                                             CAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGC                                                  >  SRR4302540.3255268/1‑94 (MQ=60)

                                              AATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCA                                                 >  SRR4302540.2431026/1‑94 (MQ=60)

                                              AATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCA                                                 <  SRR4302540.7413865/94‑1 (MQ=60)

                                              AATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCA                                                 <  SRR4302540.4054507/94‑1 (MQ=60)

                                              AATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGGGATCATCCTGTGCAGGAACTCACCGCTGGGATCTGCATAAGCGGCATTGGAGGCA                                                 >  SRR4302540.3651103/1‑94 (MQ=60)

                                               ATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAT                                                <  SRR4302540.4177687/94‑1 (MQ=60)

                                                TTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATT                                               <  SRR4302540.9860251/94‑1 (MQ=60)

                                                TTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATT                                               >  SRR4302540.5344972/1‑94 (MQ=60)

                                                  AACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGC                                             <  SRR4302540.6139560/94‑1 (MQ=60)

                                                    CGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTT                                           <  SRR4302540.9287004/94‑1 (MQ=60)

                                                     GCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTA                                          >  SRR4302540.9008404/1‑94 (MQ=60)

                                                     GCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTA                                          <  SRR4302540.3517535/94‑1 (MQ=60)

                                                     GCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTA                                          >  SRR4302540.6140281/1‑94 (MQ=60)

                                                      CATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAA                                         >  SRR4302540.5407000/1‑94 (MQ=60)

                                                       ATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAA                                        >  SRR4302540.1916726/1‑94 (MQ=60)

                                                       ATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAA                                        >  SRR4302540.1955002/1‑94 (MQ=60)

                                                        TACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAAT                                       <  SRR4302540.2500928/94‑1 (MQ=60)

                                                         ACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATC                                      >  SRR4302540.4649510/1‑94 (MQ=60)

                                                           AGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGC                                    >  SRR4302540.6927489/1‑94 (MQ=60)

                                                            GATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCG                                   <  SRR4302540.2374101/94‑1 (MQ=60)

                                                            GATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCC                                   >  SRR4302540.7444594/1‑94 (MQ=60)

                                                             ATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGC                                  >  SRR4302540.1049441/1‑94 (MQ=60)

                                                               ACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTC                                >  SRR4302540.2411504/1‑94 (MQ=60)

                                                               ACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTC                                >  SRR4302540.10137755/1‑94 (MQ=60)

                                                                 CACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAG                              <  SRR4302540.8330029/94‑1 (MQ=60)

                                                                 CACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAG                              >  SRR4302540.154058/1‑94 (MQ=60)

                                                                  ACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGG                             <  SRR4302540.5004047/94‑1 (MQ=60)

                                                                  ACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGG                             >  SRR4302540.9837604/1‑94 (MQ=60)

                                                                  ACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGG                             <  SRR4302540.5431424/94‑1 (MQ=60)

                                                                   CAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGT                            <  SRR4302540.9510208/94‑1 (MQ=60)

                                                                    AAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTG                           <  SRR4302540.5104518/94‑1 (MQ=60)

                                                                     AGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGC                          >  SRR4302540.309453/1‑94 (MQ=60)

                                                                     AGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGC                          <  SRR4302540.2063041/94‑1 (MQ=60)

                                                                      GCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCC                         >  SRR4302540.1511925/1‑94 (MQ=60)

                                                                      GCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCC                         >  SRR4302540.7697823/1‑94 (MQ=60)

                                                                        TGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCCTTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAG                       >  SRR4302540.8174568/1‑94 (MQ=60)

                                                                        TGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAG                       >  SRR4302540.2785464/1‑94 (MQ=60)

                                                                           TGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCA                    <  SRR4302540.6928526/94‑1 (MQ=60)

                                                                            GCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCAC                   <  SRR4302540.2863333/94‑1 (MQ=60)

                                                                            GCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCAC                   <  SRR4302540.6346987/94‑1 (MQ=60)

                                                                              TGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTG                 >  SRR4302540.5795314/1‑94 (MQ=60)

                                                                              TGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTG                 <  SRR4302540.3478882/94‑1 (MQ=60)

                                                                               GGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGG                >  SRR4302540.4887140/1‑94 (MQ=60)

                                                                                GCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGT               >  SRR4302540.5557389/1‑94 (MQ=60)

                                                                                GCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGT               <  SRR4302540.5503831/94‑1 (MQ=60)

                                                                                GCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAAGTGCCAGCCACTGGT               >  SRR4302540.5557390/1‑94 (MQ=60)

                                                                                   GTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCA            <  SRR4302540.7352224/94‑1 (MQ=60)

                                                                                   GTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCA            >  SRR4302540.5139119/1‑94 (MQ=60)

                                                                                   GTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCA            <  SRR4302540.4265717/94‑1 (MQ=60)

                                                                                    TGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAG           >  SRR4302540.1281404/1‑94 (MQ=60)

                                                                                     GATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGC          >  SRR4302540.1963206/1‑94 (MQ=60)

                                                                                     GATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGC          >  SRR4302540.1963205/1‑94 (MQ=60)

                                                                                     GATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGC          >  SRR4302540.2870514/1‑94 (MQ=60)

                                                                                     GATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGC          >  SRR4302540.2870515/1‑94 (MQ=60)

                                                                                      ATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCG         >  SRR4302540.2735122/1‑94 (MQ=60)

                                                                                       TCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGC        <  SRR4302540.2804129/94‑1 (MQ=60)

                                                                                        CATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCA       >  SRR4302540.8183116/1‑94 (MQ=60)

                                                                                         ATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCAC      >  SRR4302540.862339/1‑94 (MQ=60)

                                                                                          TCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACT     >  SRR4302540.1623892/1‑94 (MQ=60)

                                                                                             TGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAA  >  SRR4302540.2736528/1‑94 (MQ=60)

GTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAA  >  NC_000913/1173890‑1174076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`