Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,527	A→G	K293R (AAG→AGG)	ycfU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,527	0	A	G	99.6%	764.2 / ‑4.7	240	K293R (AAG→AGG)	ycfU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (115/124); total (115/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

Only 100 of 250 total aligned reads displayed.

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

aaaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGc                                                                                         <  2:5857373/93‑1 (MQ=255)

 aaaaaaTATCATGGGTTTACTTCTGAGCCTGATTGTCGCCGTGGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCa                                                                                        <  1:2758881/93‑1 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    >  1:10022929/1‑93 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    >  1:6354706/1‑93 (MQ=255)

        atgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGaa                                                                                 <  1:7298312/93‑1 (MQ=255)

         tgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAg                                                                                <  2:7032867/93‑1 (MQ=255)

         tgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAg                                                                                >  1:9343776/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:1054049/93‑1 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               >  1:9786229/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               >  1:2807290/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:3153620/93‑1 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:6051850/93‑1 (MQ=255)

            tgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTTATGGTAATGGAGAGGCAGGGCGAAGTAg                                                                             >  1:8943075/1‑93 (MQ=255)

            tgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAg                                                                             <  1:2282092/93‑1 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  1:9912254/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  1:4790943/1‑93 (MQ=255)

                 ttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCGCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATc                                                                        >  1:8462226/1‑93 (MQ=255)

                 ttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATc                                                                        <  1:5242872/93‑1 (MQ=255)

                  tACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATcc                                                                       >  2:8887529/1‑93 (MQ=255)

                   aCTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCt                                                                      >  2:3937465/1‑93 (MQ=255)

                     cgctgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGc                                                                    <  2:7309181/92‑1 (MQ=255)

                      tcggAGCCGGATTGTCGGCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCa                                                                   <  1:7825263/90‑1 (MQ=255)

                      gctgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCa                                                                   >  2:5246362/1‑93 (MQ=255)

                        tgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaa                                                                 >  1:4683322/1‑93 (MQ=255)

                        tgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaa                                                                 >  2:9787905/1‑93 (MQ=255)

                         gAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTCCAAAc                                                                >  2:6071670/1‑93 (MQ=255)

                           gCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGc                                                              <  2:2454108/93‑1 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGACGTAGCGATCCTGCAAACGCaa                                                            <  1:1788199/93‑1 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCaa                                                            <  1:2866243/93‑1 (MQ=255)

                              tGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAg                                                           >  2:7561495/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          >  2:3178373/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          <  1:4853296/93‑1 (MQ=255)

                                aTTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGc                                                         <  2:3019857/93‑1 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  2:7966436/1‑93 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  1:9013180/1‑93 (MQ=255)

                                   gTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTa                                                      >  2:1537021/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:8328652/93‑1 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:1975076/93‑1 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     >  2:4187268/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:5537343/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCCCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  2:5344186/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  1:1332203/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  2:1916322/93‑1 (MQ=255)

                                        cGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTcc                                                 >  2:3117615/1‑93 (MQ=255)

                                         gTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCcg                                                <  2:8189382/93‑1 (MQ=255)

                                            gcggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGa                                             <  2:3540422/93‑1 (MQ=255)

                                             cggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGAc                                            <  2:5619708/93‑1 (MQ=255)

                                                cgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACaaa                                         <  2:8945589/93‑1 (MQ=255)

                                                  tttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAta                                       >  1:493713/1‑92 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCGAGGCTTAACTCCGCGACAAATCa                                      >  2:5521320/1‑93 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      <  1:4081955/93‑1 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      >  2:6279885/1‑93 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      <  2:8322731/93‑1 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGGAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      >  1:3221970/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     >  1:698429/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     >  1:2466564/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     <  1:5113429/93‑1 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     <  1:8802379/93‑1 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTGAACCCCGCGACAAATCaa                                     >  2:7717521/1‑92 (MQ=255)

                                                     aaCAATAATACCTCACTAGAACCGATGGTAATGGAGAGGCAAGGCGAAGTAGCGATCCTGCAAACGCAAGAATTACCTCCGCGACAAATCaag                                    <  2:9232994/93‑3 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   >  2:6181668/1‑93 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   <  1:8688688/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCattat                                  <  2:5159084/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  2:4710609/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  1:4342895/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  1:3599993/93‑1 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  1:7450033/1‑93 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  1:2267652/1‑93 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  2:5691135/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  1:797020/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  2:9090801/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  1:7538049/1‑93 (MQ=255)

                                                          tattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGt                               >  2:559076/1‑93 (MQ=255)

                                                           attaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTc                              <  2:2764383/93‑1 (MQ=255)

                                                            ttaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCt                             <  1:7950666/93‑1 (MQ=255)

                                                               ccTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                          <  2:5942712/93‑1 (MQ=255)

                                                                 tCACTAGGGCTGATGGTAATGGAGAGGCATGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATg                        <  2:7425870/93‑1 (MQ=255)

                                                                  cACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATgg                       <  1:5646321/93‑1 (MQ=255)

                                                                  cACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATgg                       <  2:6892134/93‑1 (MQ=255)

                                                                    cTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTg                     >  1:5127409/1‑93 (MQ=255)

                                                                      aGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTACCTCCGCGACAAATCATGATGGTCTTTATGGTGCa                   <  1:7833157/93‑1 (MQ=255)

                                                                      aGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTCCa                   >  2:8503544/1‑93 (MQ=255)

                                                                         gCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgg                <  2:5938606/93‑1 (MQ=255)

                                                                            gATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGc             >  1:6158331/1‑93 (MQ=255)

                                                                             aTGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGcc            >  2:2317830/1‑93 (MQ=255)

                                                                             aTGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGcc            >  1:9762348/1‑93 (MQ=255)

                                                                              tGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCa           <  1:9415622/93‑1 (MQ=255)

                                                                               ggTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAg          <  2:3563567/93‑1 (MQ=255)

                                                                                gTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc         <  2:4364509/93‑1 (MQ=255)

                                                                                gTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc         <  1:1346959/93‑1 (MQ=255)

                                                                                 tAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcg        >  2:4573455/1‑93 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       <  2:4645696/93‑1 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       >  2:6619547/1‑93 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       >  2:9387672/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  2:199271/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  1:5337460/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  2:1027546/1‑93 (MQ=255)

                                                                                      gagaGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCggg   >  1:6292761/1‑93 (MQ=255)

                                                                                      gagaGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCggg   >  2:1221243/1‑93 (MQ=255)

                                                                                       agagGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGa  <  1:3193158/93‑1 (MQ=255)

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTAT  >  NC_000913/1175434‑1175620

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               >  SRR4302540.8284531/1‑94 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               <  SRR4302540.5485565/94‑1 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               <  SRR4302540.4667442/94‑1 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               >  SRR4302540.8284532/1‑94 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               >  SRR4302540.8284533/1‑94 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               <  SRR4302540.1370123/94‑1 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAG                                                                                               <  SRR4302540.7571752/94‑1 (MQ=60)

 GGAAAAAAATATGATGGGTGTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGG                                                                                              <  SRR4302540.7067766/94‑1 (MQ=60)

  GGAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGC                                                                                             >  SRR4302540.1485608/1‑94 (MQ=60)

   AAAAAAATATGATGGTTTTACGGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTATCATTATTCCCTCACTAGGGCTGATGGTAATGGAGAGGCA                                                                                            <  SRR4302540.9007294/94‑1 (MQ=60)

    AAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAG                                                                                           <  SRR4302540.811536/94‑1 (MQ=60)

    AAAAAATATCATGGGTTTACTTCTGAGCCTGATTGTCGCCGTGGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAG                                                                                           <  SRR4302540.2788687/94‑1 (MQ=60)

     AAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGG                                                                                          <  SRR4302540.9406366/94‑1 (MQ=60)

     AAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGG                                                                                          >  SRR4302540.2798811/1‑94 (MQ=60)

       AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                        <  SRR4302540.8036943/94‑1 (MQ=60)

       AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                        >  SRR4302540.6425474/1‑94 (MQ=60)

       AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                        <  SRR4302540.9912023/94‑1 (MQ=60)

       AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                        >  SRR4302540.10139563/1‑94 (MQ=60)

        AATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCG                                                                                       >  SRR4302540.2707058/1‑94 (MQ=60)

         ATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGA                                                                                      >  SRR4302540.6681122/1‑94 (MQ=60)

          TATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGT‑ATGGAGAGGCAGGGCGAAG                                                                                    <  SRR4302540.4876252/94‑1 (MQ=60)

           ATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAG                                                                                    <  SRR4302540.7379767/94‑1 (MQ=60)

           ATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAG                                                                                    <  SRR4302540.7916782/94‑1 (MQ=60)

           ATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAG                                                                                    >  SRR4302540.9455098/1‑94 (MQ=60)

           ATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAG                                                                                    <  SRR4302540.9809146/94‑1 (MQ=60)

            TGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGT                                                                                   >  SRR4302540.2332605/1‑94 (MQ=60)

            TGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGT                                                                                   >  SRR4302540.2837609/1‑94 (MQ=60)

            TGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGT                                                                                   >  SRR4302540.9900896/1‑94 (MQ=60)

            TGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGA                                                                                   >  SRR4302540.7085829/1‑94 (MQ=60)

             GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTNATGGAGAGGCAGGGCGAANTA                                                                                  >  SRR4302540.433039/1‑94 (MQ=60)

             GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTG                                                                                  <  SRR4302540.5605736/94‑1 (MQ=60)

             GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                  <  SRR4302540.3187950/94‑1 (MQ=60)

             GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                  <  SRR4302540.6119105/94‑1 (MQ=60)

             GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                  <  SRR4302540.1064780/94‑1 (MQ=60)

              atgcgtttctcGCTGAGCCTGATTGTCGCTGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAG                                                                                 <  SRR4302540.6710871/83‑1 (MQ=60)

              ATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTTATGGTAATGGAGAGGCAGGGCGAAGTAG                                                                                 >  SRR4302540.9048134/1‑94 (MQ=60)

              ATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAG                                                                                 >  SRR4302540.4529149/1‑94 (MQ=60)

              ATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAG                                                                                 <  SRR4302540.9512688/94‑1 (MQ=60)

               TGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGC                                                                                <  SRR4302540.2306612/94‑1 (MQ=60)

               TGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGC                                                                                >  SRR4302540.9870832/1‑94 (MQ=60)

               TGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGC                                                                                >  SRR4302540.5376208/1‑94 (MQ=60)

               TGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGC                                                                                >  SRR4302540.3203198/1‑94 (MQ=60)

                GGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCG                                                                               <  SRR4302540.7507988/94‑1 (MQ=60)

                GGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCG                                                                               <  SRR4302540.770960/94‑1 (MQ=60)

                  GTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAT                                                                             <  SRR4302540.526808/94‑1 (MQ=60)

                  GTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAT                                                                             >  SRR4302540.10028028/1‑94 (MQ=60)

                  GTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAT                                                                             >  SRR4302540.4842185/1‑94 (MQ=60)

                   TTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCGCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATC                                                                            >  SRR4302540.8560485/1‑94 (MQ=60)

                    TTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCC                                                                           <  SRR4302540.5300063/94‑1 (MQ=60)

                    TTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCC                                                                           <  SRR4302540.2150944/94‑1 (MQ=60)

                     TACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCT                                                                          >  SRR4302540.9037517/1‑94 (MQ=60)

                     TACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCT                                                                          <  SRR4302540.2803120/94‑1 (MQ=60)

                        TGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCA                                                                       >  SRR4302540.6214734/1‑94 (MQ=60)

                         tcgGAGCCGGATTGTCGGCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAA                                                                      <  SRR4302540.7916708/91‑1 (MQ=60)

                          CTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGGAGTAGCGATCCTGCAAA                                                                     >  SRR4302540.4957346/1‑94 (MQ=60)

                          CTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAA                                                                     >  SRR4302540.4733419/1‑94 (MQ=60)

                           TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                    >  SRR4302540.5578519/1‑94 (MQ=60)

                           TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                    <  SRR4302540.5710019/94‑1 (MQ=60)

                           TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATAATTACCTAACTAGGGCTGATGGTAATGAAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                    <  SRR4302540.9101054/94‑1 (MQ=60)

                                CTGATTGTCGCTGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                               <  SRR4302540.5426695/94‑1 (MQ=60)

                                CTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGACGTAGCGATCCTGCAAACGCAAG                                                               <  SRR4302540.1807629/94‑1 (MQ=60)

                                CTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                               <  SRR4302540.1593857/94‑1 (MQ=60)

                                CTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                               <  SRR4302540.2897350/94‑1 (MQ=60)

                                CTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                               >  SRR4302540.1028851/1‑94 (MQ=60)

                                 TGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGG                                                              >  SRR4302540.8504719/1‑94 (MQ=60)

                                  GATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGC                                                             <  SRR4302540.8880467/94‑1 (MQ=60)

                                  GATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGC                                                             <  SRR4302540.4905315/94‑1 (MQ=60)

                                  GATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGC                                                             <  SRR4302540.4905314/94‑1 (MQ=60)

                                   ATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCT                                                            >  SRR4302540.9121311/1‑94 (MQ=60)

                                   ATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCT                                                            >  SRR4302540.2199797/1‑94 (MQ=60)

                                     TGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTA                                                          <  SRR4302540.4994805/94‑1 (MQ=60)

                                      GTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAA                                                         >  SRR4302540.6698677/1‑94 (MQ=60)

                                      GTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAA                                                         <  SRR4302540.4464246/94‑1 (MQ=60)

                                       TCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAC                                                        <  SRR4302540.5598331/94‑1 (MQ=60)

                                       TCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAC                                                        <  SRR4302540.8425316/94‑1 (MQ=60)

                                       TCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAC                                                        <  SRR4302540.1996507/94‑1 (MQ=60)

                                        CGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                       <  SRR4302540.1346132/94‑1 (MQ=60)

                                        CGCCGTTGCCGCGTTTAACATTCTTACCTCGCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                       <  SRR4302540.1345830/94‑1 (MQ=60)

                                          CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                     <  SRR4302540.1331408/94‑1 (MQ=60)

                                           CGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCG                                                    <  SRR4302540.5879961/94‑1 (MQ=60)

                                              TGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCAGCGA                                                 <  SRR4302540.630427/94‑1 (MQ=60)

                                                 GGCGTTTAACATTATTACCTCACTAGGGCTGATTGGAACGGAGAGGCACTGCGAAGTAGTGATCCTGCAtaagcaacacttaactgcgcgagca                                              >  SRR4302540.4516302/1‑69 (MQ=30)

                                                   CGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAT                                            <  SRR4302540.2897996/94‑1 (MQ=60)

                                                    GTTTAACATTATTTCCTCGCTAGGGCTGATGGTAAGGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATC                                           <  SRR4302540.5026049/94‑1 (MQ=60)

                                                    GTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATC                                           >  SRR4302540.3980651/1‑94 (MQ=60)

                                                    GTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATC                                           >  SRR4302540.5183874/1‑94 (MQ=60)

                                                    GTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATA                                           >  SRR4302540.499005/1‑94 (MQ=60)

                                                     TTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGTGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCA                                          >  SRR4302540.3256988/1‑94 (MQ=60)

                                                     TTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCA                                          >  SRR4302540.3256985/1‑94 (MQ=60)

                                                     TTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGGAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCA                                          >  SRR4302540.3256987/1‑94 (MQ=60)

                                                      TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                         >  SRR4302540.705921/1‑94 (MQ=60)

                                                      TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                         <  SRR4302540.5178975/94‑1 (MQ=60)

                                                      TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                         <  SRR4302540.4125972/94‑1 (MQ=60)

                                                      TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                         >  SRR4302540.2493111/1‑94 (MQ=60)

                                                       TAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATG                                        <  SRR4302540.8904139/94‑1 (MQ=60)

                                                       TAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATG                                        <  SRR4302540.5168780/94‑1 (MQ=60)

                                                         ACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGAT                                      <  SRR4302540.8789570/94‑1 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     >  SRR4302540.2291983/1‑94 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     <  SRR4302540.6250056/94‑1 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     <  SRR4302540.3639332/94‑1 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     >  SRR4302540.7533071/1‑94 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     >  SRR4302540.2291980/1‑94 (MQ=60)

                                                          CATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATG                                     <  SRR4302540.4390168/94‑1 (MQ=60)

                                                           ATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGG                                    >  SRR4302540.7622484/1‑94 (MQ=60)

                                                           ATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGG                                    >  SRR4302540.805484/1‑94 (MQ=60)

                                                            TTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGT                                   <  SRR4302540.4031293/94‑1 (MQ=60)

                                                               TTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTcaaaccgcaaggctaaattcgccgacaatcctgaatgtcctt                                >  SRR4302540.7253265/1‑52 (MQ=60)

                                                               TTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTT                                <  SRR4302540.8043346/94‑1 (MQ=60)

                                                                 TCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTA                              <  SRR4302540.8379143/94‑1 (MQ=60)

                                                                   CTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATG                            >  SRR4302540.1979699/1‑94 (MQ=60)

                                                                   CTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATG                            >  SRR4302540.477197/1‑94 (MQ=60)

                                                                    TCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGG                           >  SRR4302540.8693221/1‑94 (MQ=60)

                                                                    TCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGG                           >  SRR4302540.8693222/1‑94 (MQ=60)

                                                                     CACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGT                          <  SRR4302540.3251670/94‑1 (MQ=60)

                                                                     CACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGT                          <  SRR4302540.5708465/94‑1 (MQ=60)

                                                                      ACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTG                         >  SRR4302540.5182942/1‑94 (MQ=60)

                                                                         AGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTACCTCCGCGACAAATCATGATGGTCTTTATGGTGCAA                      <  SRR4302540.7924683/94‑1 (MQ=60)

                                                                            GCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGG                   <  SRR4302540.2340348/94‑1 (MQ=60)

                                                                             CTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGG                  >  SRR4302540.804065/1‑94 (MQ=60)

                                                                             CTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGG                  >  SRR4302540.6409149/1‑94 (MQ=60)

                                                                              TGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGC                 >  SRR4302540.9880255/1‑94 (MQ=60)

                                                                              TGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGC                 >  SRR4302540.6226943/1‑94 (MQ=60)

                                                                               GATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCC                <  SRR4302540.8035589/94‑1 (MQ=60)

                                                                               GATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCC                >  SRR4302540.9876849/1‑94 (MQ=60)

                                                                                 TGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAG              <  SRR4302540.9527552/94‑1 (MQ=60)

                                                                                   GTACTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCG            <  SRR4302540.1361106/94‑1 (MQ=60)

                                                                                   GTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCG            <  SRR4302540.1361107/94‑1 (MQ=60)

                                                                                   GTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCG            <  SRR4302540.738785/94‑1 (MQ=60)

                                                                                     AATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCC          <  SRR4302540.9918976/94‑1 (MQ=60)

                                                                                     AATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCC          >  SRR4302540.9221366/1‑94 (MQ=60)

                                                                                     AATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCC          <  SRR4302540.2847524/94‑1 (MQ=60)

                                                                                     AATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCC          >  SRR4302540.7848481/1‑94 (MQ=60)

                                                                                      ATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCG         >  SRR4302540.5395974/1‑94 (MQ=60)

                                                                                        GGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGG       >  SRR4302540.7556003/1‑94 (MQ=60)

                                                                                        GGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGG       >  SRR4302540.6362823/1‑94 (MQ=60)

                                                                                         GAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA      >  SRR4302540.2956029/1‑94 (MQ=60)

                                                                                          AGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGAT     <  SRR4302540.3227837/94‑1 (MQ=60)

                                                                                          AGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGAT     >  SRR4302540.3995043/1‑94 (MQ=60)

                                                                                            AGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA   <  SRR4302540.8842621/94‑1 (MQ=60)

                                                                                            AGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA   <  SRR4302540.5762520/94‑1 (MQ=60)

                                                                                             GGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTAT  <  SRR4302540.9439241/94‑1 (MQ=60)

TGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTAT  >  NC_000913/1175434‑1175620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`