Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,527	A→G	K293R (AAG→AGG)	ycfU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,527	0	A	G	100.0%	797.8 / NA	247	K293R (AAG→AGG)	ycfU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (123/124); total (123/124)

Only 100 of 253 total aligned reads displayed.

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

aaaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGc                                                                                         <  1:3313131/93‑1 (MQ=255)

aaaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGc                                                                                         >  1:1349617/1‑93 (MQ=255)

aaaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGc                                                                                         >  1:5503031/1‑93 (MQ=255)

 aaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCa                                                                                        <  1:4685993/93‑1 (MQ=255)

 aaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCa                                                                                        <  1:14530/93‑1 (MQ=255)

  aaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAg                                                                                       <  1:822178/93‑1 (MQ=255)

  aaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAg                                                                                       <  1:2549114/93‑1 (MQ=255)

   aaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAgg                                                                                      <  1:2116638/93‑1 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    <  2:2426991/93‑1 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    <  1:5009393/93‑1 (MQ=255)

      atatGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCg                                                                                   <  1:2258991/93‑1 (MQ=255)

        atgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGaa                                                                                 >  1:3866259/1‑93 (MQ=255)

        atgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGaa                                                                                 <  2:4234079/93‑1 (MQ=255)

         tgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAg                                                                                <  2:2646736/93‑1 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               >  2:5980911/1‑93 (MQ=255)

           atgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTa                                                                              <  1:2495987/93‑1 (MQ=255)

           atgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTa                                                                              >  1:3550955/1‑93 (MQ=255)

               gTTTACTGCTGAGACTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGa                                                                          >  2:2386895/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  1:2278396/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         <  1:2082548/93‑1 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  2:6018623/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  2:2869254/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  2:1294257/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         <  1:230916/93‑1 (MQ=255)

                  tACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATcc                                                                       <  1:1656492/93‑1 (MQ=255)

                  tACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATcc                                                                       >  1:1736678/1‑93 (MQ=255)

                   aCTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCt                                                                      >  2:917548/1‑93 (MQ=255)

                      gctgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGGAGCGATCCTGCa                                                                   >  2:1018122/1‑93 (MQ=255)

                        tgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaa                                                                 <  1:3656261/93‑1 (MQ=255)

                         gAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaag                                                                >  2:5430790/1‑92 (MQ=255)

                         gAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAc                                                                >  1:2928393/1‑93 (MQ=255)

                           gCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGc                                                              >  1:3565189/1‑93 (MQ=255)

                            ccTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCa                                                             >  2:1883302/1‑93 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGGGGCAGGGCGAAGTAGCGATCCTGCAAACGCaa                                                            <  1:2389644/93‑1 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCaa                                                            >  2:2710899/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          <  2:1636549/93‑1 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          >  1:307098/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCCGCGTTTAACATTATTACCTCACTAGGGCTGGTGGTAATGGAGAGGCAGGGCGAAGTAGCGGTCCTGCCAACGCaacg                                                          >  2:1674425/1‑91 (MQ=255)

                                aTTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGc                                                         <  2:4927034/93‑1 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  2:1461554/1‑93 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        <  2:783165/93‑1 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  1:4039808/1‑93 (MQ=255)

                                   gTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTa                                                      <  1:2343765/93‑1 (MQ=255)

                                   gTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTa                                                      >  2:300269/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     >  1:5630335/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     >  2:887598/1‑93 (MQ=255)

                                      gCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACt                                                   >  2:3293156/1‑93 (MQ=255)

                                        cGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTcc                                                 <  1:1145222/93‑1 (MQ=255)

                                        cGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTcc                                                 >  1:3256442/1‑93 (MQ=255)

                                          ttGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCcgc                                               >  2:5918354/1‑93 (MQ=255)

                                          ttGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCCTAACTCcgc                                               >  1:2705261/1‑93 (MQ=255)

                                             cggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGAc                                            <  1:6257909/93‑1 (MQ=255)

                                              ggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACa                                           >  2:2481238/1‑93 (MQ=255)

                                              ggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACa                                           >  1:586330/1‑93 (MQ=255)

                                              ggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAACCTGCAAAACCAAGGCTTTACTCCGCGACa                                           >  2:2174658/1‑93 (MQ=255)

                                               gcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACaa                                          >  2:4031188/1‑93 (MQ=255)

                                                cgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACaaa                                         >  2:5382675/1‑93 (MQ=255)

                                                 gTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAt                                        >  1:3063789/1‑93 (MQ=255)

                                                 gTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAt                                        <  2:1418117/93‑1 (MQ=255)

                                                  tttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATc                                       <  2:4713361/93‑1 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     >  1:1190640/1‑93 (MQ=255)

                                                     aaCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatg                                    >  1:2056944/1‑93 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   >  2:1352755/1‑93 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   <  2:5835324/93‑1 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   >  2:3231629/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                <  2:170705/93‑1 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  2:41047/1‑93 (MQ=255)

                                                         tgattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                <  1:5159796/91‑1 (MQ=255)

                                                          tattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGt                               <  1:6017347/93‑1 (MQ=255)

                                                           attaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTc                              >  2:1532336/1‑93 (MQ=255)

                                                           attaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTc                              <  2:5490813/93‑1 (MQ=255)

                                                           attaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTc                              <  2:4605014/93‑1 (MQ=255)

                                                            ttaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCt                             <  2:5144972/93‑1 (MQ=255)

                                                              aCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCttt                           >  1:948096/1‑93 (MQ=255)

                                                              aCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCttt                           >  1:3739966/1‑93 (MQ=255)

                                                              aCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCttt                           >  2:5321771/1‑93 (MQ=255)

                                                               ccTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                          <  1:2156374/93‑1 (MQ=255)

                                                               ccTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                          >  1:2963560/1‑93 (MQ=255)

                                                               ccTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                          <  1:4794679/93‑1 (MQ=255)

                                                                cTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAt                         >  2:3340754/1‑93 (MQ=255)

                                                                cTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAt                         >  1:4446144/1‑93 (MQ=255)

                                                                 tCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATg                        >  2:5691971/1‑93 (MQ=255)

                                                                  cACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATgg                       <  1:2827460/93‑1 (MQ=255)

                                                                   aCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGt                      <  2:1111695/93‑1 (MQ=255)

                                                                     tAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGc                    >  2:4791564/1‑93 (MQ=255)

                                                                     tAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGc                    <  1:497230/93‑1 (MQ=255)

                                                                     tAGGACTGATGGTAATGGAGAGGCAAGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGc                    <  2:5758669/93‑1 (MQ=255)

                                                                      aGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCa                   >  1:1908924/1‑93 (MQ=255)

                                                                       gggCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCaa                  <  2:3155723/93‑1 (MQ=255)

                                                                       gggCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCaa                  >  1:5423830/1‑93 (MQ=255)

                                                                         gCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgg                <  2:3769029/93‑1 (MQ=255)

                                                                         gCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgg                <  1:1302647/93‑1 (MQ=255)

                                                                            gATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGc             >  1:4257336/1‑93 (MQ=255)

                                                                                gTAATGGAGAGGCAGGGCTAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc         <  1:5237690/93‑1 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       >  2:358103/1‑93 (MQ=255)

                                                                                   aTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGcc      >  2:6091613/1‑93 (MQ=255)

                                                                                     ggAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCgg    >  1:3919859/1‑93 (MQ=255)

                                                                                     ggAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCgg    <  2:6279540/93‑1 (MQ=255)

                                                                                     ggAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCgg    <  1:565089/93‑1 (MQ=255)

                                                                                     ggAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCgg    <  2:3110025/93‑1 (MQ=255)

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA  >  NC_000913/1175435‑1175619

GGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGG                                                                                             <  SRR4302533.4587582/94‑1 (MQ=60)

GGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGG                                                                                             >  SRR4302533.414087/1‑94 (MQ=60)

 GAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGC                                                                                            >  SRR4302533.1357735/1‑94 (MQ=60)

 GAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGC                                                                                            >  SRR4302533.5536234/1‑94 (MQ=60)

  AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCA                                                                                           <  SRR4302533.3333587/94‑1 (MQ=60)

  AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCA                                                                                           >  SRR4302533.4642106/1‑94 (MQ=60)

   AAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAG                                                                                          <  SRR4302533.4714380/94‑1 (MQ=60)

   AAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAG                                                                                          <  SRR4302533.14634/94‑1 (MQ=60)

    AAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGG                                                                                         <  SRR4302533.827004/94‑1 (MQ=60)

    AAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGG                                                                                         <  SRR4302533.2564806/94‑1 (MQ=60)

     AAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGG                                                                                        <  SRR4302533.2129694/94‑1 (MQ=60)

     AAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGG                                                                                        <  SRR4302533.262081/94‑1 (MQ=60)

      AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                       >  SRR4302533.3019316/1‑94 (MQ=60)

      AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                       >  SRR4302533.1373855/1‑94 (MQ=60)

      AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                       <  SRR4302533.2237846/94‑1 (MQ=60)

      AAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGC                                                                                       <  SRR4302533.3215962/94‑1 (MQ=60)

       AATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCG                                                                                      <  SRR4302533.5039741/94‑1 (MQ=60)

       AATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCG                                                                                      <  SRR4302533.2204804/94‑1 (MQ=60)

        ATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGA                                                                                     <  SRR4302533.2272968/94‑1 (MQ=60)

        ATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGA                                                                                     >  SRR4302533.3742685/1‑94 (MQ=60)

         TATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAA                                                                                    >  SRR4302533.3889862/1‑94 (MQ=60)

         TATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAA                                                                                    >  SRR4302533.5792349/1‑94 (MQ=60)

           TGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGT                                                                                  <  SRR4302533.3920519/94‑1 (MQ=60)

            GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                 >  SRR4302533.3572830/1‑94 (MQ=60)

            GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                 <  SRR4302533.5869656/94‑1 (MQ=60)

            GATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTA                                                                                 >  SRR4302533.6038828/1‑94 (MQ=60)

             ATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAG                                                                                <  SRR4302533.2511384/94‑1 (MQ=60)

              TGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGC                                                                               <  SRR4302533.3791646/94‑1 (MQ=60)

               GGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCG                                                                              <  SRR4302533.4363000/94‑1 (MQ=60)

                GGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGA                                                                             <  SRR4302533.5236712/94‑1 (MQ=60)

                 GTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAT                                                                            >  SRR4302533.2292505/1‑94 (MQ=60)

                  TTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATC                                                                           <  SRR4302533.2095364/94‑1 (MQ=60)

                  TTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATC                                                                           <  SRR4302533.232346/94‑1 (MQ=60)

                  TTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATC                                                                           <  SRR4302533.364098/94‑1 (MQ=60)

                   TTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCC                                                                          <  SRR4302533.4102105/94‑1 (MQ=60)

                   TTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCC                                                                          >  SRR4302533.1747217/1‑94 (MQ=60)

                    TACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCT                                                                         <  SRR4302533.4693642/94‑1 (MQ=60)

                    TACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCT                                                                         <  SRR4302533.1666517/94‑1 (MQ=60)

                    TACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCT                                                                         <  SRR4302533.2460886/94‑1 (MQ=60)

                     ACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTG                                                                        <  SRR4302533.3111799/94‑1 (MQ=60)

                      CTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGC                                                                       >  SRR4302533.3951224/1‑94 (MQ=60)

                      CTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGCAATGGAGAGGCAGGGCGAAGTAGCGATCCTGC                                                                       >  SRR4302533.2354390/1‑94 (MQ=60)

                       TGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCA                                                                      <  SRR4302533.5359368/94‑1 (MQ=60)

                       TGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCA                                                                      >  SRR4302533.2489272/1‑94 (MQ=60)

                         CTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAA                                                                    >  SRR4302533.3330249/1‑94 (MQ=60)

                          TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                   >  SRR4302533.3576376/1‑94 (MQ=60)

                          TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                   >  SRR4302533.2946306/1‑94 (MQ=60)

                          TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                   <  SRR4302533.1836392/94‑1 (MQ=60)

                          TGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAAC                                                                   <  SRR4302533.3678735/94‑1 (MQ=60)

                            AGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGC                                                                 >  SRR4302533.3587161/1‑94 (MQ=60)

                             GCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATTCTGCAAACGCA                                                                >  SRR4302533.271813/1‑94 (MQ=60)

                               CTGATTGTCGCGGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                              <  SRR4302533.2274148/94‑1 (MQ=60)

                               CTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGGGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAG                                                              <  SRR4302533.2404401/94‑1 (MQ=60)

                                TGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGG                                                             >  SRR4302533.308977/1‑94 (MQ=60)

                                  ATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCT                                                           >  SRR4302533.4064393/1‑94 (MQ=60)

                                  ATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCT                                                           <  SRR4302533.1446579/94‑1 (MQ=60)

                                    TGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTA                                                         <  SRR4302533.4869572/94‑1 (MQ=60)

                                     GTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAA                                                        <  SRR4302533.2358273/94‑1 (MQ=60)

                                     GTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAA                                                        >  SRR4302533.5664383/1‑94 (MQ=60)

                                       CGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                      >  SRR4302533.25130/1‑94 (MQ=60)

                                       CGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                      <  SRR4302533.5569345/94‑1 (MQ=60)

                                       CGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                      <  SRR4302533.3065350/94‑1 (MQ=60)

                                       CGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACT                                                      >  SRR4302533.933882/1‑94 (MQ=60)

                                         CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                    <  SRR4302533.3853735/94‑1 (MQ=60)

                                         CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                    <  SRR4302533.3471308/94‑1 (MQ=60)

                                         CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                    >  SRR4302533.6266826/1‑94 (MQ=60)

                                         CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                    <  SRR4302533.1181248/94‑1 (MQ=60)

                                         CCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCC                                                    >  SRR4302533.3276523/1‑94 (MQ=60)

                                          CGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCG                                                   <  SRR4302533.1152057/94‑1 (MQ=60)

                                          CGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCG                                                   >  SRR4302533.1533225/1‑94 (MQ=60)

                                          CGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCG                                                   >  SRR4302533.2367885/1‑94 (MQ=60)

                                          CGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCG                                                   <  SRR4302533.2956229/94‑1 (MQ=60)

                                           GTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCCTAACTCCGC                                                  >  SRR4302533.2721854/1‑94 (MQ=60)

                                            TTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCG                                                 <  SRR4302533.4878625/94‑1 (MQ=60)

                                             TGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGA                                                <  SRR4302533.5090067/94‑1 (MQ=60)

                                             TGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGA                                                >  SRR4302533.2008758/1‑94 (MQ=60)

                                             TGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGA                                                >  SRR4302533.5529966/1‑94 (MQ=60)

                                               CGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACA                                              >  SRR4302533.2942111/1‑94 (MQ=60)

                                               CGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACA                                              >  SRR4302533.589790/1‑94 (MQ=60)

                                               CGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACA                                              <  SRR4302533.6295332/94‑1 (MQ=60)

                                                 GCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAA                                            <  SRR4302533.1313088/94‑1 (MQ=60)

                                                 GCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAA                                            >  SRR4302533.2099279/1‑94 (MQ=60)

                                                  CGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAT                                           >  SRR4302533.3082601/1‑94 (MQ=60)

                                                     TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                        >  SRR4302533.1197795/1‑94 (MQ=60)

                                                     TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                        <  SRR4302533.2886908/94‑1 (MQ=60)

                                                     TTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCAT                                        >  SRR4302533.3673443/1‑94 (MQ=60)

                                                      TAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATG                                       >  SRR4302533.2069630/1‑94 (MQ=60)

                                                          ATTATTCCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACGCCGCGACAAATCATGATGG                                   <  SRR4302533.2374874/94‑1 (MQ=60)

                                                           TTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGT                                  <  SRR4302533.4125815/94‑1 (MQ=60)

                                                           TGATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGT                                  <  SRR4302533.5190938/94‑1 (MQ=60)

                                                            TATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTC                                 <  SRR4302533.6053806/94‑1 (MQ=60)

                                                             ATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCT                                >  SRR4302533.3083780/1‑94 (MQ=60)

                                                               TACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTT                              >  SRR4302533.3762904/1‑94 (MQ=60)

                                                               TACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTT                              >  SRR4302533.953667/1‑94 (MQ=60)

                                                                ACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAagggtttctctccgcgagaaaaaattatatgttt                             >  SRR4302533.3658467/1‑60 (MQ=60)

                                                                ACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTA                             >  SRR4302533.2981678/1‑94 (MQ=60)

                                                                 CCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAT                            <  SRR4302533.2169650/94‑1 (MQ=60)

                                                                 CCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAT                            >  SRR4302533.4473036/1‑94 (MQ=60)

                                                                 CCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAT                            <  SRR4302533.4718867/94‑1 (MQ=60)

                                                                 CCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTAT                            <  SRR4302533.4823724/94‑1 (MQ=60)

                                                                  CTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATG                           >  SRR4302533.2106082/1‑94 (MQ=60)

                                                                  CTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATG                           >  SRR4302533.2306125/1‑94 (MQ=60)

                                                                   TCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGG                          <  SRR4302533.969538/94‑1 (MQ=60)

                                                                   TCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGG                          <  SRR4302533.504866/94‑1 (MQ=60)

                                                                    CACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGT                         <  SRR4302533.2844857/94‑1 (MQ=60)

                                                                    CACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGT                         <  SRR4302533.1666732/94‑1 (MQ=60)

                                                                    CACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGT                         >  SRR4302533.1985578/1‑94 (MQ=60)

                                                                     ACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTG                        >  SRR4302533.4029352/1‑94 (MQ=60)

                                                                     ACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGGCTTTATGGTG                        >  SRR4302533.1210303/1‑94 (MQ=60)

                                                                       TAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCA                      <  SRR4302533.500192/94‑1 (MQ=60)

                                                                       TAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCA                      >  SRR4302533.1920569/1‑94 (MQ=60)

                                                                        AGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAA                     >  SRR4302533.5456496/1‑94 (MQ=60)

                                                                         GGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAG                    <  SRR4302533.2131085/94‑1 (MQ=60)

                                                                          GGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAGCTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGG                   <  SRR4302533.5679292/94‑1 (MQ=60)

                                                                          GGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGG                   <  SRR4302533.5016986/94‑1 (MQ=60)

                                                                           GCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGG                  <  SRR4302533.2092690/94‑1 (MQ=60)

                                                                           GCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGG                  <  SRR4302533.1310478/94‑1 (MQ=60)

                                                                            CTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGG                 <  SRR4302533.274749/94‑1 (MQ=60)

                                                                             TGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGC                >  SRR4302533.4283002/1‑94 (MQ=60)

                                                                             TGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGC                >  SRR4302533.2318065/1‑94 (MQ=60)

                                                                              GATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCC               <  SRR4302533.3419346/94‑1 (MQ=60)

                                                                               ATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCA              <  SRR4302533.5970650/94‑1 (MQ=60)

                                                                               ATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCA              <  SRR4302533.1304222/94‑1 (MQ=60)

                                                                               ATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATCGTGCAAGGGGCCA              >  SRR4302533.1696053/1‑94 (MQ=60)

                                                                                  GTAATGGAGAGGCAGGGCTAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCG           <  SRR4302533.5269179/94‑1 (MQ=60)

                                                                                    AATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCC         >  SRR4302533.6168012/1‑94 (MQ=60)

                                                                                      TGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGG       <  SRR4302533.5383977/94‑1 (MQ=60)

                                                                                      TGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGG       >  SRR4302533.3943797/1‑94 (MQ=60)

                                                                                      TGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGG       <  SRR4302533.931037/94‑1 (MQ=60)

                                                                                       GGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGG      <  SRR4302533.568438/94‑1 (MQ=60)

                                                                                       GGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGG      >  SRR4302533.5612008/1‑94 (MQ=60)

                                                                                         AGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGAT    <  SRR4302533.768398/94‑1 (MQ=60)

                                                                                           AGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA  >  SRR4302533.2526166/1‑94 (MQ=60)

                                                                                           AGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA  >  SRR4302533.1826477/1‑94 (MQ=60)

GGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTA  >  NC_000913/1175435‑1175619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`