Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,617 T→C V232A (GTG→GCG)  oppB → oligopeptide transport permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,6170TC100.0% 772.5 / NA 243V232A (GTG→GCG) oppBoligopeptide transport permease protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (140/103);  total (140/103)

Only 100 of 252 total aligned reads displayed.

ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706
                                                                                          |                                                                                         
  tCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctc                                                                                       <  1:5750540/93‑1 (MQ=255)
    cAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctc                                                                                     >  2:5394463/1‑93 (MQ=255)
    cAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctc                                                                                     <  2:3472319/93‑1 (MQ=255)
     aaTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctct                                                                                    >  1:8145037/1‑93 (MQ=255)
     aaTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctct                                                                                    >  1:4008223/1‑93 (MQ=255)
      aTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   <  1:9315306/93‑1 (MQ=255)
      aTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   <  1:5684870/93‑1 (MQ=255)
       ttttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTta                                                                                  <  1:3124687/93‑1 (MQ=255)
        tttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtat                                                                                 >  2:3078555/1‑93 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  1:7874295/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  2:2943306/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  2:9052908/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  1:8219669/93‑1 (MQ=255)
          tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTatat                                                                               <  1:3714238/93‑1 (MQ=255)
            ttCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATgg                                                                             >  1:8591921/1‑93 (MQ=255)
              tgTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCCCGGATTAAAACCTGCTCTGTTTCCCCCCCTTTCTTATATGGGc                                                                           <  2:746412/92‑1 (MQ=255)
              cGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGc                                                                           <  2:9278706/93‑1 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          <  2:2745390/93‑1 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  2:7993088/1‑93 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  2:1000339/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  1:6142075/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  2:9626703/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  1:7940076/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         <  1:9026808/93‑1 (MQ=255)
                  cTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACTCGCGCTCTCTTATATGGGCCCCg                                                                       >  2:7219418/1‑93 (MQ=255)
                  cTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCg                                                                       >  1:104052/1‑93 (MQ=255)
                   tGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCga                                                                      >  1:1712644/1‑92 (MQ=255)
                   tGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGc                                                                      >  2:5977438/1‑93 (MQ=255)
                      ccGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCAtt                                                                   >  1:4262245/1‑93 (MQ=255)
                      ccGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCAtt                                                                   >  1:5613855/1‑93 (MQ=255)
                       cGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCAttt                                                                  <  1:8745234/93‑1 (MQ=255)
                        gggCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTg                                                                 <  2:6612154/93‑1 (MQ=255)
                        gggCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTg                                                                 >  2:3695926/1‑93 (MQ=255)
                            gAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTACAACCTGGTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTAgg                                                             >  2:1259722/1‑93 (MQ=255)
                            gAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCgg                                                             >  1:2963223/1‑93 (MQ=255)
                            gAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCACGCATTTGTCgg                                                             >  2:9806619/1‑93 (MQ=255)
                                gggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCatt                                                         <  2:1234082/93‑1 (MQ=255)
                                 ggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCatta                                                        <  2:765775/93‑1 (MQ=255)
                                  gTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattat                                                       >  2:4856674/1‑93 (MQ=255)
                                  gTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattat                                                       >  2:3436445/1‑93 (MQ=255)
                                  gTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattat                                                       >  2:1547941/1‑93 (MQ=255)
                                  gTTACCTATGCCGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattat                                                       <  1:5610605/93‑1 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattattc                                                     >  1:8190678/1‑92 (MQ=255)
                                     tccTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAc                                                    <  1:3423137/92‑1 (MQ=255)
                                     aCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAc                                                    >  2:2625646/1‑93 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 >  1:2065394/1‑93 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 >  1:6506725/1‑93 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGt                                                >  2:9486805/1‑93 (MQ=255)
                                          tGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGtt                                               <  1:3791016/93‑1 (MQ=255)
                                           gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTc                                              <  2:8571935/93‑1 (MQ=255)
                                            cggcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCt                                             <  2:8659434/93‑1 (MQ=255)
                                             ggcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTa                                            >  2:5580479/1‑93 (MQ=255)
                                             ggcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTa                                            <  2:8896027/93‑1 (MQ=255)
                                              gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAt                                           <  2:8877476/93‑1 (MQ=255)
                                              gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAt                                           >  1:9553780/1‑93 (MQ=255)
                                               cggATCATTTTACGTTACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATg                                          <  1:7518833/93‑1 (MQ=255)
                                               cggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATg                                          <  1:4744084/93‑1 (MQ=255)
                                                ggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATgg                                         >  2:1893202/1‑93 (MQ=255)
                                                ggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTAACCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGCTCTATgg                                         >  1:3035100/1‑93 (MQ=255)
                                                 gATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGt                                        >  1:6927080/1‑93 (MQ=255)
                                                 gATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGt                                        >  1:2658333/1‑93 (MQ=255)
                                                  aTCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGtt                                       >  2:7907030/1‑93 (MQ=255)
                                                   tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTa                                      >  1:5440002/1‑93 (MQ=255)
                                                   tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTa                                      <  2:6144372/93‑1 (MQ=255)
                                                   tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTa                                      >  1:1887148/1‑93 (MQ=255)
                                                   tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTa                                      <  2:3445680/93‑1 (MQ=255)
                                                    cATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTAt                                     <  1:9721345/93‑1 (MQ=255)
                                                     aTTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATc                                    >  1:3747472/1‑93 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCg                                   >  2:6766459/1‑93 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCg                                   >  1:1318584/1‑93 (MQ=255)
                                                        ttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGaa                                 >  2:3295767/1‑93 (MQ=255)
                                                            gTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCa                             <  2:4531840/93‑1 (MQ=255)
                                                             tCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCAt                            <  1:2395949/93‑1 (MQ=255)
                                                             tCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGGTCTATGGTTATCGAAACCAt                            <  2:9684015/93‑1 (MQ=255)
                                                                cGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTATCGGTTCTATGGTTATTGTAACCATTTa                         >  2:5671818/1‑93 (MQ=255)
                                                                cGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTa                         <  1:3973053/93‑1 (MQ=255)
                                                                cGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTa                         >  1:7355077/1‑93 (MQ=255)
                                                                cGCAGTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTa                         <  2:8160208/93‑1 (MQ=255)
                                                                  cATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATg                       <  1:2167607/93‑1 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATTGTTATTGAAACCATTTATgg                      >  2:1962954/1‑93 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                      >  1:5279411/1‑93 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                      >  2:9877828/1‑93 (MQ=255)
                                                                    ttAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                     >  1:2723068/1‑93 (MQ=255)
                                                                    ttAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                     >  2:6894212/1‑93 (MQ=255)
                                                                    ttAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                     >  1:8266609/1‑93 (MQ=255)
                                                                     tAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGtt                    >  2:2879484/1‑93 (MQ=255)
                                                                       aaaCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGtgtg                  >  2:1329240/1‑93 (MQ=255)
                                                                       aaaCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTg                  >  2:4947987/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             <  1:610606/93‑1 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  2:390500/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  1:4383432/1‑93 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  1:5124018/93‑1 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  2:4313450/93‑1 (MQ=255)
                                                                               tctGTTTCCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          <  2:63271/93‑1 (MQ=255)
                                                                                 tGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTg        <  2:4689415/93‑1 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       >  1:304598/1‑93 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       >  1:5457810/1‑93 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       <  1:7037548/93‑1 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       >  1:1552822/1‑93 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       >  1:7703987/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTT  >  NC_000913/1301526‑1301710
                                                                                           |                                                                                             
CACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGC                                                                                             >  SRR4302540.757336/1‑94 (MQ=60)
  CTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTC                                                                                           >  SRR4302540.3152081/1‑94 (MQ=60)
   TCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCT                                                                                          <  SRR4302540.5814009/94‑1 (MQ=60)
     CAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCT                                                                                        >  SRR4302540.4051378/1‑94 (MQ=60)
     CAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCT                                                                                        >  SRR4302540.8239587/1‑94 (MQ=60)
      AATTTTATTCGTACTGCCCGGGCGCCAGGGTTACCTATGCGGCGTAACATTTTACGTCACGCATTAAAACCTTCTCTGTTACGCCCGCTCTCTT                                                                                       >  SRR4302540.4064932/1‑94 (MQ=60)
      AATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTT                                                                                       >  SRR4302540.6426302/1‑94 (MQ=60)
       ATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTA                                                                                      <  SRR4302540.5747458/94‑1 (MQ=60)
       ATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTA                                                                                      >  SRR4302540.3874668/1‑94 (MQ=60)
       ATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTA                                                                                      <  SRR4302540.9426478/94‑1 (MQ=60)
        TTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTAT                                                                                     <  SRR4302540.3158812/94‑1 (MQ=60)
          TTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATAT                                                                                   <  SRR4302540.7966276/94‑1 (MQ=60)
          TTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATAT                                                                                   <  SRR4302540.3735252/94‑1 (MQ=60)
          TTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATAT                                                                                   <  SRR4302540.8315209/94‑1 (MQ=60)
           TATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATG                                                                                  <  SRR4302540.3754770/94‑1 (MQ=60)
           TATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATG                                                                                  <  SRR4302540.3754769/94‑1 (MQ=60)
           TATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATG                                                                                  >  SRR4302540.8997037/1‑94 (MQ=60)
            ATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGG                                                                                 >  SRR4302540.831881/1‑94 (MQ=60)
            ATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGG                                                                                 >  SRR4302540.8691803/1‑94 (MQ=60)
                GTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCC                                                                             >  SRR4302540.8032609/1‑94 (MQ=60)
                GTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCC                                                                             >  SRR4302540.6210480/1‑94 (MQ=60)
                 TACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCC                                                                            <  SRR4302540.9135128/94‑1 (MQ=60)
                 TACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCC                                                                            <  SRR4302540.307590/94‑1 (MQ=60)
                 TACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCC                                                                            <  SRR4302540.307583/94‑1 (MQ=60)
                  ACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCG                                                                           >  SRR4302540.105160/1‑94 (MQ=60)
                  ACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCG                                                                           <  SRR4302540.5650440/94‑1 (MQ=60)
                   CTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGA                                                                          >  SRR4302540.1731148/1‑94 (MQ=60)
                      CCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATT                                                                       >  SRR4302540.5675613/1‑94 (MQ=60)
                      CCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATT                                                                       >  SRR4302540.4308604/1‑94 (MQ=60)
                      CCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATT                                                                       >  SRR4302540.5675612/1‑94 (MQ=60)
                       CCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTT                                                                      >  SRR4302540.795749/1‑94 (MQ=60)
                        CGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTG                                                                     <  SRR4302540.809157/94‑1 (MQ=60)
                        CGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTG                                                                     <  SRR4302540.8846726/94‑1 (MQ=60)
                        CGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTG                                                                     >  SRR4302540.7090804/1‑94 (MQ=60)
                          GGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTC                                                                   <  SRR4302540.3003715/94‑1 (MQ=60)
                           GCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCG                                                                  <  SRR4302540.1563796/94‑1 (MQ=60)
                           GCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCG                                                                  <  SRR4302540.7430898/94‑1 (MQ=60)
                            CGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGG                                                                 >  SRR4302540.2995908/1‑94 (MQ=60)
                            CGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGG                                                                 >  SRR4302540.4494714/1‑94 (MQ=60)
                              AAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCA                                                               >  SRR4302540.4370965/1‑94 (MQ=60)
                               AAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCAT                                                              >  SRR4302540.8400537/1‑94 (MQ=60)
                                   GTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATT                                                          <  SRR4302540.5672325/94‑1 (MQ=60)
                                   GTTACCTATGCCGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATT                                                          <  SRR4302540.5672327/94‑1 (MQ=60)
                                    TTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTC                                                         >  SRR4302540.8285854/1‑94 (MQ=60)
                                    TTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTA                                                         >  SRR4302540.8285855/1‑94 (MQ=60)
                                      TCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACC                                                       <  SRR4302540.3460356/94‑1 (MQ=60)
                                      ACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACC                                                       <  SRR4302540.2280010/94‑1 (MQ=60)
                                        CTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGG                                                     >  SRR4302540.2087616/1‑94 (MQ=60)
                                        CTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGG                                                     >  SRR4302540.6579039/1‑94 (MQ=60)
                                        CTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGG                                                     >  SRR4302540.9449264/1‑94 (MQ=60)
                                         TATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGT                                                    >  SRR4302540.880258/1‑94 (MQ=60)
                                         TATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGT                                                    >  SRR4302540.4457149/1‑94 (MQ=60)
                                           TGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTC                                                  <  SRR4302540.3832349/94‑1 (MQ=60)
                                            GCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCT                                                 >  SRR4302540.5507542/1‑94 (MQ=60)
                                              GGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCTGCCTTATTACCGGTTATAT                                               >  SRR4302540.4028144/1‑94 (MQ=60)
                                              GGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAT                                               >  SRR4302540.1688760/1‑94 (MQ=60)
                                              GGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAT                                               >  SRR4302540.9666777/1‑94 (MQ=60)
                                               GCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATG                                              >  SRR4302540.530471/1‑94 (MQ=60)
                                                CGGATCATTTTACGTTACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGG                                             <  SRR4302540.7602911/94‑1 (MQ=60)
                                                CGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGG                                             <  SRR4302540.4794708/94‑1 (MQ=60)
                                                CGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTAACCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGCTCTATGG                                             >  SRR4302540.3068408/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            <  SRR4302540.9773457/94‑1 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.2687179/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.7986347/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.2047841/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.8133332/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.6658033/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.7004171/1‑94 (MQ=60)
                                                 GGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGT                                            >  SRR4302540.1361660/1‑94 (MQ=60)
                                                   ATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTA                                          >  SRR4302540.5499855/1‑94 (MQ=60)
                                                   ATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTA                                          >  SRR4302540.1907809/1‑94 (MQ=60)
                                                   ATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTA                                          <  SRR4302540.575256/94‑1 (MQ=60)
                                                    TCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTAT                                         >  SRR4302540.5339896/1‑94 (MQ=60)
                                                     CATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCTGCATTATTACCGGTTCTATGGTTATC                                        >  SRR4302540.9919454/1‑94 (MQ=60)
                                                     CATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATC                                        <  SRR4302540.9835545/94‑1 (MQ=60)
                                                     CATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATC                                        >  SRR4302540.3788365/1‑94 (MQ=60)
                                                     CATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATC                                        <  SRR4302540.3785500/94‑1 (MQ=60)
                                                      ATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCG                                       <  SRR4302540.8270890/94‑1 (MQ=60)
                                                      ATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCG                                       >  SRR4302540.1332307/1‑94 (MQ=60)
                                                      ATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCG                                       >  SRR4302540.1332308/1‑94 (MQ=60)
                                                       TTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGA                                      >  SRR4302540.7579297/1‑94 (MQ=60)
                                                       TTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGA                                      >  SRR4302540.4812276/1‑94 (MQ=60)
                                                        TTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAA                                     >  SRR4302540.3216277/1‑94 (MQ=60)
                                                         TTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAA                                    >  SRR4302540.9138096/1‑94 (MQ=60)
                                                          TACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAAC                                   >  SRR4302540.6188993/1‑94 (MQ=60)
                                                             GTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCAT                                >  SRR4302540.4102307/1‑94 (MQ=60)
                                                             GTCACGCATTAAAACCTGCTCGGTTACGCGCGCTCTCTTATATGGGCCCCGCATTTGGCGTCATTATTACCGGTTCTATGGTTATCGAAACCAT                                >  SRR4302540.4102306/1‑94 (MQ=60)
                                                              TCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATT                               <  SRR4302540.2421768/94‑1 (MQ=60)
                                                               CACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTT                              >  SRR4302540.1788066/1‑94 (MQ=60)
                                                               CACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTT                              >  SRR4302540.1788067/1‑94 (MQ=60)
                                                                ACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTA                             >  SRR4302540.7437106/1‑94 (MQ=60)
                                                                 CGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAT                            <  SRR4302540.4015874/94‑1 (MQ=60)
                                                                 CGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAT                            <  SRR4302540.2935924/94‑1 (MQ=60)
                                                                  GCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATG                           >  SRR4302540.3223982/1‑94 (MQ=60)
                                                                   CATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGG                          <  SRR4302540.2714957/94‑1 (MQ=60)
                                                                   CATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGG                          <  SRR4302540.2190973/94‑1 (MQ=60)
                                                                   CATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGG                          >  SRR4302540.5337072/1‑94 (MQ=60)
                                                                    ATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGT                         >  SRR4302540.6058740/1‑94 (MQ=60)
                                                                    ATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGT                         >  SRR4302540.8362661/1‑94 (MQ=60)
                                                                    ATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGT                         >  SRR4302540.2752427/1‑94 (MQ=60)
                                                                     TTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTT                        <  SRR4302540.8673066/94‑1 (MQ=60)
                                                                      TAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTT                       <  SRR4302540.2128884/94‑1 (MQ=60)
                                                                       AAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTG                      <  SRR4302540.7669268/94‑1 (MQ=60)
                                                                       AAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTG                      <  SRR4302540.5179897/94‑1 (MQ=60)
                                                                         AACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCC                    <  SRR4302540.5344517/94‑1 (MQ=60)
                                                                          ACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCG                   >  SRR4302540.9117680/1‑94 (MQ=60)
                                                                           CCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGG                  >  SRR4302540.3791717/1‑94 (MQ=60)
                                                                            CTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGG                 >  SRR4302540.968551/1‑94 (MQ=60)
                                                                            CTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGG                 >  SRR4302540.4431026/1‑94 (MQ=60)
                                                                            CTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGG                 >  SRR4302540.2506032/1‑94 (MQ=60)
                                                                            CTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACAGGCTCTATGGTTATCGAAACCATTTATGGTTTGCCGGG                 >  SRR4302540.7051208/1‑94 (MQ=60)
                                                                             TGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGG                <  SRR4302540.617130/94‑1 (MQ=60)
                                                                             TGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGG                >  SRR4302540.2841755/1‑94 (MQ=60)
                                                                             TGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAAACATTTATGGTTTGGCGGGG                >  SRR4302540.2181893/1‑94 (MQ=60)
                                                                              GCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGA               <  SRR4302540.4284667/94‑1 (MQ=60)
                                                                               CTCTGTTGCCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAT              <  SRR4302540.9577559/94‑1 (MQ=60)
                                                                               CTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAT              <  SRR4302540.5179512/94‑1 (MQ=60)
                                                                                TCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATT             <  SRR4302540.1336246/94‑1 (MQ=60)
                                                                                TCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATT             <  SRR4302540.2604017/94‑1 (MQ=60)
                                                                                 CTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCAGGGGATTG            >  SRR4302540.8298348/1‑94 (MQ=60)
                                                                                  TGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGG           >  SRR4302540.307625/1‑94 (MQ=60)
                                                                                  TGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGG           >  SRR4302540.5517879/1‑94 (MQ=60)
                                                                                  TGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGG           >  SRR4302540.1569918/1‑94 (MQ=60)
                                                                                  TGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGG           >  SRR4302540.7792156/1‑94 (MQ=60)
                                                                                  TGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGG           >  SRR4302540.7792155/1‑94 (MQ=60)
                                                                                   TTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCAAAACCATTTATGGTTTGCCGGGGATTGGA          <  SRR4302540.6135373/94‑1 (MQ=60)
                                                                                   GTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGA          <  SRR4302540.3921229/94‑1 (MQ=60)
                                                                                   GTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGA          <  SRR4302540.7115781/94‑1 (MQ=60)
                                                                                    TTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGAC         >  SRR4302540.5682282/1‑94 (MQ=60)
                                                                                    TTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGGTATCGAAACCATTTATGGTTTGCCGGGGATTGGAA         >  SRR4302540.5682281/1‑94 (MQ=60)
                                                                                      ACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAA       <  SRR4302540.6335186/94‑1 (MQ=60)
                                                                                         CGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTG    <  SRR4302540.4888690/94‑1 (MQ=60)
                                                                                         CGCGCTCTCTTATATGGGCCCCGCATTGGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTG    <  SRR4302540.4888689/94‑1 (MQ=60)
                                                                                           CGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTT  >  SRR4302540.7888654/1‑94 (MQ=60)
                                                                                           |                                                                                             
CACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTT  >  NC_000913/1301526‑1301710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑