Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,330,421 A→G L450L (TTA→TTG)  topA → DNA topoisomerase type I omega protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,4210AG99.0% 935.0 / ‑3.1 291L450L (TTA→TTG) topADNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base G (166/122);  total (169/122)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 294 total aligned reads displayed.

CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGC  >  NC_000913/1330331‑1330510
                                                                                          |                                                                                         
ccGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGcc                                                                                         >  1:8692757/1‑93 (MQ=255)
    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                     >  1:9176661/1‑93 (MQ=255)
    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                     >  1:4111279/1‑93 (MQ=255)
     tgcaaGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAGAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                    <  2:8970488/90‑1 (MQ=255)
     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                    >  1:8597369/1‑93 (MQ=255)
     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                    >  2:2220959/1‑93 (MQ=255)
     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                    <  2:1803510/93‑1 (MQ=255)
      gAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGt                                                                                   >  2:5006166/1‑93 (MQ=255)
          gCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaat                                                                               <  2:2539744/93‑1 (MQ=255)
            aCGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaataa                                                                             <  2:459817/93‑1 (MQ=255)
            aCGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaataa                                                                             >  1:9443984/1‑93 (MQ=255)
            aCGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaataa                                                                             >  2:7342679/1‑93 (MQ=255)
             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            <  1:9812981/93‑1 (MQ=255)
             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            <  2:3365986/93‑1 (MQ=255)
             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            <  2:4396534/93‑1 (MQ=255)
             cgcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATaaa                                                                            >  1:7581185/1‑93 (MQ=255)
              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                           >  1:3012782/1‑93 (MQ=255)
              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                           <  1:7427652/93‑1 (MQ=255)
               cgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAgg                                                                          >  1:5566645/1‑93 (MQ=255)
               cgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAgg                                                                          >  1:5837355/1‑93 (MQ=255)
                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                        >  1:9696632/1‑93 (MQ=255)
                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                       <  2:9202188/93‑1 (MQ=255)
                    gTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATg                                                                     >  2:5661744/1‑93 (MQ=255)
                    gTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATg                                                                     >  2:7833012/1‑93 (MQ=255)
                    gTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATg                                                                     >  2:372340/1‑93 (MQ=255)
                    gTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATg                                                                     >  2:7096452/1‑93 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    >  2:7993556/1‑93 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    <  2:9669611/93‑1 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    <  2:2142579/93‑1 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                    >  1:9249699/1‑93 (MQ=255)
                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                  >  2:4831737/1‑93 (MQ=255)
                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                  >  1:3196655/1‑93 (MQ=255)
                        tttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctct                                                                 <  1:7750925/93‑1 (MQ=255)
                        tttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctct                                                                 <  2:328436/93‑1 (MQ=255)
                          tGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGa                                                               >  1:5379014/1‑93 (MQ=255)
                           gCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAc                                                              <  2:4263527/93‑1 (MQ=255)
                            cGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACg                                                             <  1:9042814/93‑1 (MQ=255)
                            cGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACg                                                             >  1:6477401/1‑93 (MQ=255)
                             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGc                                                            >  1:7600328/1‑93 (MQ=255)
                             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGc                                                            <  1:4930333/93‑1 (MQ=255)
                              ttttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCt                                                           >  1:1652125/1‑93 (MQ=255)
                               tttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTc                                                          <  2:6325890/93‑1 (MQ=255)
                               tttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTc                                                          >  1:4990847/1‑93 (MQ=255)
                                 tGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGt                                                        <  2:9870752/93‑1 (MQ=255)
                                 tGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGt                                                        <  2:1265537/93‑1 (MQ=255)
                                   aTGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTg                                                      >  2:3702002/1‑93 (MQ=255)
                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                     <  2:5835177/93‑1 (MQ=255)
                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                     >  2:3996790/1‑93 (MQ=255)
                                     ggCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGac                                                    >  2:5210820/1‑92 (MQ=255)
                                      gCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAAc                                                   >  1:2529512/1‑93 (MQ=255)
                                        tGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACtt                                                 >  1:103729/1‑93 (MQ=255)
                                         ggACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTa                                                <  1:2414722/93‑1 (MQ=255)
                                          gACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTac                                               <  2:823187/93‑1 (MQ=255)
                                          gACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTac                                               >  2:2378852/1‑93 (MQ=255)
                                            cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                             <  2:943083/93‑1 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                          >  1:4266632/1‑93 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                          >  2:6557682/1‑93 (MQ=255)
                                                  tGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagccc                                       >  1:6409539/1‑93 (MQ=255)
                                                     tGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcccagc                                    >  2:6263537/1‑93 (MQ=255)
                                                         tgcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACtt                                >  2:7537401/1‑93 (MQ=255)
                                                         tgcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACtt                                >  1:1177952/1‑93 (MQ=255)
                                                         tgcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACtt                                >  1:2162353/1‑93 (MQ=255)
                                                            gttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAc                             >  1:1440246/1‑93 (MQ=255)
                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATGAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCCGCACTTTAcc                            >  1:3887779/1‑93 (MQ=255)
                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAcc                            >  2:1379591/1‑93 (MQ=255)
                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAcc                            >  1:1995443/1‑93 (MQ=255)
                                                              tgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCa                           <  1:7259411/93‑1 (MQ=255)
                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                          >  1:4407988/1‑93 (MQ=255)
                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                          >  1:6811653/1‑93 (MQ=255)
                                                                  tAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTGCCAAgcc                       <  2:3633184/93‑1 (MQ=255)
                                                                  tAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgcc                       <  1:3717160/93‑1 (MQ=255)
                                                                  tAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgcc                       >  2:4656486/1‑93 (MQ=255)
                                                                     aGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgccgcc                    <  2:1970810/93‑1 (MQ=255)
                                                                      ggCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                   >  1:8912261/1‑93 (MQ=255)
                                                                      ggCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                   >  1:7882603/1‑93 (MQ=255)
                                                                      ggCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                   >  1:4873915/1‑93 (MQ=255)
                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                >  1:6882324/1‑93 (MQ=255)
                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                >  2:2997152/1‑93 (MQ=255)
                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                <  2:617714/93‑1 (MQ=255)
                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                >  1:5179152/1‑93 (MQ=255)
                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc               >  2:836033/1‑93 (MQ=255)
                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc               >  2:3551679/1‑93 (MQ=255)
                                                                           tGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg              >  2:1585367/1‑93 (MQ=255)
                                                                           tGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg              >  1:4615469/1‑93 (MQ=255)
                                                                           tGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg              >  1:5145731/1‑93 (MQ=255)
                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            >  1:1772527/1‑93 (MQ=255)
                                                                             aaGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt            <  2:3147746/93‑1 (MQ=255)
                                                                               gATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc          <  1:6631607/93‑1 (MQ=255)
                                                                               gATCGCATCTTACCAGCAGTTAATAAAGTCGAGGCTCTGACGCTCGTTGAACTTACACCAGCTCAGCACTTTACCAAGCGGCAAGCCAGTTTc          >  2:5412756/1‑93 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACGAAGCCGCCAGCCCGTTTCa         >  1:6004036/1‑93 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCa         >  2:6753503/1‑93 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCa         >  2:5998172/1‑93 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCa         <  1:2565158/93‑1 (MQ=255)
                                                                                 tCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAg        <  2:4523139/93‑1 (MQ=255)
                                                                                  cGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGt       >  1:3529886/1‑93 (MQ=255)
                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa     <  1:8023227/93‑1 (MQ=255)
                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGACGCCAGCCCGCTTCAGTGa     >  2:2758711/1‑93 (MQ=255)
                                                                                     aTCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa    >  1:271246/1‑93 (MQ=255)
                                                                                      tCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAg   <  2:4144348/93‑1 (MQ=255)
                                                                                      tCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAg   >  2:10011454/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGC  >  NC_000913/1330331‑1330510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCG  >  NC_000913/1330328‑1330514
                                                                                             |                                                                                             
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTG                                                                                               <  SRR4302540.9362321/94‑1 (MQ=60)
  TCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCC                                                                                             <  SRR4302540.459447/94‑1 (MQ=60)
  TCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCC                                                                                             >  SRR4302540.8793678/1‑94 (MQ=60)
  TCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCC                                                                                             >  SRR4302540.8793677/1‑94 (MQ=60)
    CGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAG                                                                                           <  SRR4302540.9386317/94‑1 (MQ=60)
     GCCTGAAAGCACGCGGTCGAATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGC                                                                                          >  SRR4302540.6896838/1‑94 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                         >  SRR4302540.4155655/1‑94 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                         >  SRR4302540.9286727/1‑94 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                         >  SRR4302540.8078334/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                        >  SRR4302540.6933052/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                        >  SRR4302540.8697298/1‑94 (MQ=60)
        TGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTTGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGT                                                                                       <  SRR4302540.3109234/94‑1 (MQ=60)
        TGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGT                                                                                       <  SRR4302540.7721389/94‑1 (MQ=60)
         GAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTT                                                                                      <  SRR4302540.1060924/94‑1 (MQ=60)
          AAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTA                                                                                     <  SRR4302540.3610082/94‑1 (MQ=60)
              CACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAA                                                                                 >  SRR4302540.9556106/1‑94 (MQ=60)
               ACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAA                                                                                >  SRR4302540.7666284/1‑94 (MQ=60)
                CGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAG                                                                               >  SRR4302540.3045916/1‑94 (MQ=60)
                CGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAG                                                                               <  SRR4302540.9927823/94‑1 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGTTGAAGATCGCATCTTGCCGGCATTTAATAAAGG                                                                              >  SRR4302540.5901903/1‑94 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                              <  SRR4302540.5220094/94‑1 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                              >  SRR4302540.5627943/1‑94 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                              >  SRR4302540.5901901/1‑94 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                              <  SRR4302540.7510396/94‑1 (MQ=60)
                  CGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGC                                                                             <  SRR4302540.2722678/94‑1 (MQ=60)
                   GGTCGTTTTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                            <  SRR4302540.6542579/94‑1 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                            >  SRR4302540.9019696/1‑94 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                            <  SRR4302540.6542578/94‑1 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                            >  SRR4302540.9810647/1‑94 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                            <  SRR4302540.276113/94‑1 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                           >  SRR4302540.9510847/1‑94 (MQ=60)
                     TCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGAT                                                                          <  SRR4302540.6160297/94‑1 (MQ=60)
                     TCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGAT                                                                          >  SRR4302540.444890/1‑94 (MQ=60)
                     TCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGAT                                                                          <  SRR4302540.4093598/94‑1 (MQ=60)
                      CGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATG                                                                         >  SRR4302540.211494/1‑94 (MQ=60)
                       GTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGC                                                                        >  SRR4302540.9360432/1‑94 (MQ=60)
                       GTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGC                                                                        >  SRR4302540.3115439/1‑94 (MQ=60)
                        ttattttcgttttgatgagtggacaaaagtgaatcctgcggtggggtAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCT                                                                       <  SRR4302540.9093527/47‑1 (MQ=60)
                         ATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTC                                                                      >  SRR4302540.990924/1‑94 (MQ=60)
                         ATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTC                                                                      >  SRR4302540.5096303/1‑94 (MQ=60)
                         ATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTC                                                                      >  SRR4302540.3231376/1‑94 (MQ=60)
                          TTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCT                                                                     >  SRR4302540.5694111/1‑94 (MQ=60)
                          TTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCT                                                                     <  SRR4302540.4860550/94‑1 (MQ=60)
                           TTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTG                                                                    <  SRR4302540.7841447/94‑1 (MQ=60)
                           TTTACGGTTTTATGGCTGGACAAAAGGTATGCCTGCCTTGCGTAAAGGCGTTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTG                                                                    <  SRR4302540.7841448/94‑1 (MQ=60)
                            TTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGA                                                                   <  SRR4302540.1944946/94‑1 (MQ=60)
                            TTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGA                                                                   >  SRR4302540.5438166/1‑94 (MQ=60)
                            TTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGA                                                                   <  SRR4302540.4252334/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                  >  SRR4302540.1794726/1‑94 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                  <  SRR4302540.52218/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                  <  SRR4302540.4723775/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                  <  SRR4302540.2517166/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                  <  SRR4302540.7670195/94‑1 (MQ=60)
                              GCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACG                                                                 >  SRR4302540.6549466/1‑94 (MQ=60)
                              GCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACG                                                                 <  SRR4302540.8877039/94‑1 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                                >  SRR4302540.7685728/1‑94 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                                <  SRR4302540.9151359/94‑1 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                               >  SRR4302540.1670135/1‑94 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                               <  SRR4302540.8790646/94‑1 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                               >  SRR4302540.7930955/1‑94 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                               <  SRR4302540.4983479/94‑1 (MQ=60)
                                 TTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGGGATGCTCTGACGCTC                                                              >  SRR4302540.3672955/1‑94 (MQ=60)
                                 TTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTC                                                              <  SRR4302540.4580798/94‑1 (MQ=60)
                                 TTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTC                                                              >  SRR4302540.5044880/1‑94 (MQ=60)
                                   TTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGT                                                            <  SRR4302540.1679674/94‑1 (MQ=60)
                                     GATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTG                                                          <  SRR4302540.4105070/94‑1 (MQ=60)
                                      ATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGA                                                         >  SRR4302540.8597410/1‑94 (MQ=60)
                                      ATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGA                                                         >  SRR4302540.9248585/1‑94 (MQ=60)
                                        GGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAAT                                                       >  SRR4302540.2556806/1‑94 (MQ=60)
                                        GGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAAC                                                       >  SRR4302540.2556805/1‑94 (MQ=60)
                                        GGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAAC                                                       >  SRR4302540.1544305/1‑94 (MQ=60)
                                          GTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTT                                                     <  SRR4302540.3247758/94‑1 (MQ=60)
                                          CTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTT                                                     >  SRR4302540.104836/1‑94 (MQ=60)
                                           TGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTA                                                    >  SRR4302540.5038341/1‑94 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                   >  SRR4302540.10105987/1‑94 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                   >  SRR4302540.5192772/1‑94 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                   <  SRR4302540.2440748/94‑1 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTCGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                   <  SRR4302540.6813191/94‑1 (MQ=60)
                                             GACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACA                                                  <  SRR4302540.7363388/94‑1 (MQ=60)
                                              ACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAC                                                 <  SRR4302540.1193073/94‑1 (MQ=60)
                                              ACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAC                                                 <  SRR4302540.3829527/94‑1 (MQ=60)
                                                AAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCA                                               <  SRR4302540.8644579/94‑1 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAG                                              <  SRR4302540.7126264/94‑1 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAG                                              >  SRR4302540.4313036/1‑94 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAG                                              >  SRR4302540.9240862/1‑94 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAC                                              >  SRR4302540.7681609/1‑94 (MQ=60)
                                                  AAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGC                                             >  SRR4302540.10067197/1‑94 (MQ=60)
                                                  AAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGC                                             >  SRR4302540.4341350/1‑94 (MQ=60)
                                                    GTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCC                                           >  SRR4302540.6480874/1‑94 (MQ=60)
                                                    GTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCC                                           <  SRR4302540.2094247/94‑1 (MQ=60)
                                                     TGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCA                                          <  SRR4302540.4630686/94‑1 (MQ=60)
                                                       ATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGC                                        >  SRR4302540.8972556/1‑94 (MQ=60)
                                                       ATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGC                                        <  SRR4302540.5321179/94‑1 (MQ=60)
                                                       ATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGC                                        >  SRR4302540.6757526/1‑94 (MQ=60)
                                                          CCTTCGTTTCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACT                                     <  SRR4302540.2638808/94‑1 (MQ=60)
                                                          CCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGATCTGACGCTCGTTGAACTTACACTAGCCCAGCACT                                     >  SRR4302540.6920223/1‑94 (MQ=60)
                                                           CTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTT                                    >  SRR4302540.2185647/1‑94 (MQ=60)
                                                           CTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTT                                    >  SRR4302540.1190128/1‑94 (MQ=60)
                                                             GCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTA                                  <  SRR4302540.2498024/94‑1 (MQ=60)
                                                              CGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAC                                 >  SRR4302540.5719240/1‑94 (MQ=60)
                                                              CGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAC                                 >  SRR4302540.1455882/1‑94 (MQ=60)
                                                               GTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATGAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCCGCACTTTACC                                >  SRR4302540.3930002/1‑94 (MQ=60)
                                                               GTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACC                                >  SRR4302540.2017065/1‑94 (MQ=60)
                                                               GTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACC                                >  SRR4302540.9817421/1‑94 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                              >  SRR4302540.6887019/1‑94 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                              >  SRR4302540.4455779/1‑94 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                              <  SRR4302540.7340413/94‑1 (MQ=60)
                                                                   CGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGC                            >  SRR4302540.7986276/1‑94 (MQ=60)
                                                                   CGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGC                            <  SRR4302540.3925498/94‑1 (MQ=60)
                                                                    GTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCC                           >  SRR4302540.6584439/1‑94 (MQ=60)
                                                                     TAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCG                          <  SRR4302540.3757715/94‑1 (MQ=60)
                                                                       AAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCC                        >  SRR4302540.1624351/1‑94 (MQ=60)
                                                                        AGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCA                       >  SRR4302540.6639105/1‑94 (MQ=60)
                                                                        AGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCA                       >  SRR4302540.9015468/1‑94 (MQ=60)
                                                                        AGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCA                       >  SRR4302540.7974642/1‑94 (MQ=60)
                                                                        AGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCA                       >  SRR4302540.4926213/1‑94 (MQ=60)
                                                                         GGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAG                      >  SRR4302540.2581519/1‑94 (MQ=60)
                                                                           CGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCC                    >  SRR4302540.5235519/1‑94 (MQ=60)
                                                                           CGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCC                    <  SRR4302540.9745284/94‑1 (MQ=60)
                                                                           CGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCC                    >  SRR4302540.6958616/1‑94 (MQ=60)
                                                                           CGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCC                    >  SRR4302540.4942424/1‑94 (MQ=60)
                                                                           CGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCC                    >  SRR4302540.4942422/1‑94 (MQ=60)
                                                                            GATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCC                   >  SRR4302540.7826595/1‑94 (MQ=60)
                                                                             ATGAAGATCGCATTTTGCCAGCAGTTAATAAAGGCGATGCTCTGATGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAcctcg                  >  SRR4302540.5201470/1‑89 (MQ=60)
                                                                             ATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCG                  >  SRR4302540.9349793/1‑94 (MQ=60)
                                                                             ATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCG                  >  SRR4302540.4664980/1‑94 (MQ=60)
                                                                             ATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCG                  >  SRR4302540.1423308/1‑94 (MQ=60)
                                                                             ATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCG                  >  SRR4302540.5201471/1‑94 (MQ=60)
                                                                              TGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGT                 >  SRR4302540.6014947/1‑94 (MQ=60)
                                                                               GAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTT                >  SRR4302540.5289972/1‑94 (MQ=60)
                                                                               GAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTT                >  SRR4302540.1791764/1‑94 (MQ=60)
                                                                                AAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTT               >  SRR4302540.9722093/1‑94 (MQ=60)
                                                                                 AGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTC              >  SRR4302540.1730584/1‑94 (MQ=60)
                                                                                 AGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTC              >  SRR4302540.6124440/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACGAAGCCGCCAGCCCGTTTCA             >  SRR4302540.6070592/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA             >  SRR4302540.5519248/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA             <  SRR4302540.6705012/94‑1 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA             <  SRR4302540.2265882/94‑1 (MQ=60)
                                                                                   ATCGCCTCTGGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACCCCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            <  SRR4302540.5043970/94‑1 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            >  SRR4302540.7521414/1‑94 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            <  SRR4302540.243783/94‑1 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            <  SRR4302540.2592811/94‑1 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            <  SRR4302540.9334981/94‑1 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG            >  SRR4302540.7521413/1‑94 (MQ=60)
                                                                                    TCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGT           >  SRR4302540.3568397/1‑94 (MQ=60)
                                                                                       CATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAA        >  SRR4302540.3041819/1‑94 (MQ=60)
                                                                                       CATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAA        <  SRR4302540.8116536/94‑1 (MQ=60)
                                                                                       CATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAA        >  SRR4302540.273918/1‑94 (MQ=60)
                                                                                        ATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGGTTCAGTGAAG       >  SRR4302540.5550469/1‑94 (MQ=60)
                                                                                         TCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGC      >  SRR4302540.7366537/1‑94 (MQ=60)
                                                                                          CTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCA     <  SRR4302540.3461343/94‑1 (MQ=60)
                                                                                           TTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAT    >  SRR4302540.5046078/1‑94 (MQ=60)
                                                                                           TTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAT    >  SRR4302540.1296262/1‑94 (MQ=60)
                                                                                           TTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAT    <  SRR4302540.8985056/94‑1 (MQ=60)
                                                                                           TTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAT    >  SRR4302540.5046079/1‑94 (MQ=60)
                                                                                           TTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAT    >  SRR4302540.8599622/1‑94 (MQ=60)
                                                                                            TGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC   >  SRR4302540.9521049/1‑94 (MQ=60)
                                                                                            TGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC   >  SRR4302540.9627484/1‑94 (MQ=60)
                                                                                            TGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC   >  SRR4302540.9808127/1‑94 (MQ=60)
                                                                                             GCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCG  >  SRR4302540.291036/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCG  >  NC_000913/1330328‑1330514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑