Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,330,421 A→G L450L (TTA→TTG)  topA → DNA topoisomerase type I omega protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,4210AG100.0% 698.6 / NA 214L450L (TTA→TTG) topADNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (109/105);  total (109/105)

Only 100 of 217 total aligned reads displayed.

CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAG  >  NC_000913/1330331‑1330509
                                                                                          |                                                                                        
ccGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGcc                                                                                        >  1:3969590/1‑93 (MQ=255)
 cGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCca                                                                                       <  1:747450/93‑1 (MQ=255)
  gCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcag                                                                                      >  2:1673882/1‑93 (MQ=255)
    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                    >  1:1160221/1‑93 (MQ=255)
    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                    >  1:1319631/1‑93 (MQ=255)
    cTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagca                                                                                    <  1:1548212/93‑1 (MQ=255)
     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACACAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                   >  1:3391757/1‑93 (MQ=255)
     tGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCcagcag                                                                                   >  1:1005373/1‑93 (MQ=255)
         aGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaa                                                                               >  1:6233960/1‑93 (MQ=255)
         aGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaa                                                                               <  2:3630380/93‑1 (MQ=255)
         aGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaa                                                                               >  2:4785217/1‑93 (MQ=255)
          gCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaat                                                                              >  1:773703/1‑93 (MQ=255)
          gCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaat                                                                              <  1:5062160/93‑1 (MQ=255)
           cACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaata                                                                             >  2:2086752/1‑93 (MQ=255)
           cACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaata                                                                             <  1:2148467/93‑1 (MQ=255)
           cACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaata                                                                             <  2:3829321/93‑1 (MQ=255)
            aCGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTtaaaaa                                                                            <  1:1021019/93‑1 (MQ=255)
              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                          >  2:652255/1‑93 (MQ=255)
              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                          <  1:3544344/93‑1 (MQ=255)
              gcgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAg                                                                          <  2:378657/93‑1 (MQ=255)
               cgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAgg                                                                         >  2:3923196/1‑93 (MQ=255)
                ggTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGc                                                                        >  2:1371065/1‑93 (MQ=255)
                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                       >  2:5040429/1‑93 (MQ=255)
                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                       >  1:4316469/1‑93 (MQ=255)
                 gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCg                                                                       <  1:2476314/93‑1 (MQ=255)
                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                      >  1:5435867/1‑93 (MQ=255)
                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                      <  1:5401677/93‑1 (MQ=255)
                  tCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGa                                                                      >  1:6171428/1‑93 (MQ=255)
                   cGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGAt                                                                     <  2:2939147/93‑1 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                   >  1:4704162/1‑93 (MQ=255)
                     tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGc                                                                   >  1:1449173/1‑93 (MQ=255)
                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                 >  2:1055297/1‑93 (MQ=255)
                       ttttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGctc                                                                 <  2:4256546/93‑1 (MQ=255)
                          tGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGa                                                              <  2:2512515/93‑1 (MQ=255)
                           gCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAc                                                             <  2:3203183/93‑1 (MQ=255)
                            cGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACg                                                            >  1:4357013/1‑93 (MQ=255)
                             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGc                                                           >  1:2638980/1‑93 (MQ=255)
                              ttttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCt                                                          >  2:5063983/1‑93 (MQ=255)
                              ttttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCt                                                          >  1:5678680/1‑93 (MQ=255)
                                 tGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGt                                                       <  1:1770948/93‑1 (MQ=255)
                                  gATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                      >  2:3775806/1‑93 (MQ=255)
                                  gATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                      <  2:5836321/93‑1 (MQ=255)
                                  gATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                      <  1:3840188/93‑1 (MQ=255)
                                   aTGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTg                                                     <  2:3420883/93‑1 (MQ=255)
                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                    >  2:2241963/1‑93 (MQ=255)
                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                    >  2:2882590/1‑93 (MQ=255)
                                    tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGa                                                    >  1:2978978/1‑93 (MQ=255)
                                     ggCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                   >  2:5264249/1‑93 (MQ=255)
                                        tGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACtt                                                >  2:3663087/1‑93 (MQ=255)
                                         ggACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTa                                               <  1:3763584/93‑1 (MQ=255)
                                           aCAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTaca                                             >  1:3216749/1‑93 (MQ=255)
                                           aCAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGACGATGCTCTGACGCTCGTTGAACTTaca                                             >  1:4759485/1‑93 (MQ=255)
                                            cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                            <  2:3502164/93‑1 (MQ=255)
                                            cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                            <  2:3702907/93‑1 (MQ=255)
                                            cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                            >  2:2178549/1‑93 (MQ=255)
                                             aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAcc                                           <  1:5978322/93‑1 (MQ=255)
                                              aaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAcca                                          >  1:2233992/1‑93 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         <  2:4716093/93‑1 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         >  2:79279/1‑93 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         <  1:1109720/93‑1 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         >  1:2545947/1‑93 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         >  1:4044732/1‑93 (MQ=255)
                                               aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccag                                         <  1:4899276/93‑1 (MQ=255)
                                                  tGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagccc                                      <  1:3133371/93‑1 (MQ=255)
                                                   gATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagccca                                     <  2:5735110/93‑1 (MQ=255)
                                                     tGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcccagc                                   <  2:5997559/93‑1 (MQ=255)
                                                     tGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcccagc                                   <  1:230723/93‑1 (MQ=255)
                                                      gCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCTCAACa                                  >  2:5547118/1‑93 (MQ=255)
                                                      gCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCa                                  <  2:730230/93‑1 (MQ=255)
                                                       ccTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCAc                                 >  2:1500864/1‑93 (MQ=255)
                                                       ccTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCAc                                 <  1:5415361/93‑1 (MQ=255)
                                                          gcgttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACttt                              >  2:2976469/1‑93 (MQ=255)
                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAcc                           <  1:5587326/93‑1 (MQ=255)
                                                             ttgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAcc                           >  2:3058220/1‑93 (MQ=255)
                                                              tgcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCa                          <  1:1878564/93‑1 (MQ=255)
                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                         >  1:3358422/1‑93 (MQ=255)
                                                               gcgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaa                         >  2:2384025/1‑93 (MQ=255)
                                                                cgtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAg                        <  2:519231/93‑1 (MQ=255)
                                                                cgtAAAGGCGAAGAAGATCGCATCTTGCCAGCAGTTAATAAAGGAGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCaac                        >  1:2690577/1‑92 (MQ=255)
                                                                 gtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgc                       >  2:1826511/1‑93 (MQ=255)
                                                                 gtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgc                       <  1:1176029/93‑1 (MQ=255)
                                                                 gtAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgc                       <  1:4504357/93‑1 (MQ=255)
                                                                  tAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgcc                      >  2:1465388/1‑93 (MQ=255)
                                                                    aaGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgccgc                    >  1:5276482/1‑93 (MQ=255)
                                                                     aGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAgccgcc                   <  1:5837538/93‑1 (MQ=255)
                                                                      ggCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCa                  <  1:482940/93‑1 (MQ=255)
                                                                       gCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAg                 <  2:4923114/93‑1 (MQ=255)
                                                                         gATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc               <  2:3336888/93‑1 (MQ=255)
                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc              >  2:3319985/1‑93 (MQ=255)
                                                                          aTGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGccc              >  2:3586668/1‑93 (MQ=255)
                                                                            gAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGt            >  1:4109934/1‑93 (MQ=255)
                                                                               gATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc         >  1:3361091/1‑93 (MQ=255)
                                                                               gATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc         <  1:5598231/93‑1 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCa        >  2:4361152/1‑93 (MQ=255)
                                                                                aTCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCa        >  1:572557/1‑93 (MQ=255)
                                                                                 tCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACCCCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAg       <  1:2842681/93‑1 (MQ=255)
                                                                                 tCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAg       >  2:2776285/1‑93 (MQ=255)
                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa    <  1:635340/93‑1 (MQ=255)
                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa    <  2:1176135/93‑1 (MQ=255)
                                                                                    cATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa    <  2:2209403/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAG  >  NC_000913/1330331‑1330509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC  >  NC_000913/1330328‑1330513
                                                                                             |                                                                                            
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTG                                                                                              <  SRR4302533.3869340/94‑1 (MQ=60)
 TTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGC                                                                                             >  SRR4302533.3852002/1‑94 (MQ=60)
 TTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGC                                                                                             >  SRR4302533.2650897/1‑94 (MQ=60)
  TCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCC                                                                                            >  SRR4302533.3993767/1‑94 (MQ=60)
    CGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAG                                                                                          <  SRR4302533.751851/94‑1 (MQ=60)
    CGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAG                                                                                          <  SRR4302533.142118/94‑1 (MQ=60)
    CGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAG                                                                                          <  SRR4302533.6249721/94‑1 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                        >  SRR4302533.1167184/1‑94 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                        >  SRR4302533.5804981/1‑94 (MQ=60)
      CCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCA                                                                                        >  SRR4302533.1327550/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACACAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       >  SRR4302533.3412703/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       >  SRR4302533.1011253/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       >  SRR4302533.3188138/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       >  SRR4302533.3045866/1‑94 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       <  SRR4302533.1557542/94‑1 (MQ=60)
       CTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAG                                                                                       <  SRR4302533.4912096/94‑1 (MQ=60)
          AAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTA                                                                                    <  SRR4302533.4376772/94‑1 (MQ=60)
          AAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTA                                                                                    <  SRR4302533.5910632/94‑1 (MQ=60)
           AAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAA                                                                                   >  SRR4302533.6271283/1‑94 (MQ=60)
           AAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAA                                                                                   >  SRR4302533.2885839/1‑94 (MQ=60)
            AGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAAT                                                                                  >  SRR4302533.778252/1‑94 (MQ=60)
             GCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATA                                                                                 <  SRR4302533.5092746/94‑1 (MQ=60)
              CACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAA                                                                                <  SRR4302533.2161698/94‑1 (MQ=60)
               ACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAAAAAA                                                                               <  SRR4302533.1027017/94‑1 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                             <  SRR4302533.3355701/94‑1 (MQ=60)
                 GCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGG                                                                             <  SRR4302533.3566190/94‑1 (MQ=60)
                  CGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGC                                                                            >  SRR4302533.2413097/1‑94 (MQ=60)
                  CGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGC                                                                            >  SRR4302533.5418056/1‑94 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                           >  SRR4302533.4342462/1‑94 (MQ=60)
                   GGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCG                                                                           >  SRR4302533.2209036/1‑94 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                          <  SRR4302533.2491587/94‑1 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                          >  SRR4302533.1532016/1‑94 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                          >  SRR4302533.5468602/1‑94 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                          >  SRR4302533.6208516/1‑94 (MQ=60)
                    GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGA                                                                          >  SRR4302533.4262175/1‑94 (MQ=60)
                     TCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGAT                                                                         <  SRR4302533.5434137/94‑1 (MQ=60)
                       GTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGC                                                                       <  SRR4302533.1358399/94‑1 (MQ=60)
                       GTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGC                                                                       >  SRR4302533.1457917/1‑94 (MQ=60)
                       GTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGC                                                                       >  SRR4302533.4732676/1‑94 (MQ=60)
                        TATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCT                                                                      <  SRR4302533.5080410/94‑1 (MQ=60)
                         ATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTC                                                                     >  SRR4302533.5451746/1‑94 (MQ=60)
                          TTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCT                                                                    <  SRR4302533.66927/94‑1 (MQ=60)
                            TTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGA                                                                  <  SRR4302533.5752051/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                 <  SRR4302533.5445586/94‑1 (MQ=60)
                             TGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGAC                                                                 <  SRR4302533.1179195/94‑1 (MQ=60)
                              GCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACG                                                                <  SRR4302533.5630693/94‑1 (MQ=60)
                              GCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACG                                                                >  SRR4302533.4383312/1‑94 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                               >  SRR4302533.3965559/1‑94 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                               >  SRR4302533.2959901/1‑94 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                               >  SRR4302533.13227/1‑94 (MQ=60)
                               CGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGC                                                               >  SRR4302533.2655201/1‑94 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                              >  SRR4302533.5712979/1‑94 (MQ=60)
                                GTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCT                                                              <  SRR4302533.3788633/94‑1 (MQ=60)
                                 TTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTC                                                             >  SRR4302533.3379539/1‑94 (MQ=60)
                                    TGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTT                                                          <  SRR4302533.81793/94‑1 (MQ=60)
                                    TGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTT                                                          <  SRR4302533.1781729/94‑1 (MQ=60)
                                    TGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTT                                                          >  SRR4302533.282631/1‑94 (MQ=60)
                                     GATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTG                                                         <  SRR4302533.3863649/94‑1 (MQ=60)
                                     GATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTG                                                         >  SRR4302533.629168/1‑94 (MQ=60)
                                      ATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGA                                                        >  SRR4302533.2997205/1‑94 (MQ=60)
                                      ATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGA                                                        >  SRR4302533.4922663/1‑94 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                  <  SRR4302533.3786654/94‑1 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                  <  SRR4302533.4768815/94‑1 (MQ=60)
                                            GGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTAC                                                  <  SRR4302533.6073048/94‑1 (MQ=60)
                                             GACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACA                                                 >  SRR4302533.3236581/1‑94 (MQ=60)
                                             GACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGACGATGCTCTGACGCTCGTTGAACTTACA                                                 >  SRR4302533.4788357/1‑94 (MQ=60)
                                              ACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAC                                                >  SRR4302533.1742812/1‑94 (MQ=60)
                                                AAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCA                                              <  SRR4302533.6014532/94‑1 (MQ=60)
                                                AAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCA                                              >  SRR4302533.2247775/1‑94 (MQ=60)
                                                AAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCA                                              >  SRR4302533.4076392/1‑94 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAG                                             >  SRR4302533.4069349/1‑94 (MQ=60)
                                                 AAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAG                                             >  SRR4302533.2561611/1‑94 (MQ=60)
                                                  AAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGC                                            <  SRR4302533.4928960/94‑1 (MQ=60)
                                                  AAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGC                                            <  SRR4302533.1116304/94‑1 (MQ=60)
                                                  AAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGC                                            <  SRR4302533.568348/94‑1 (MQ=60)
                                                   AGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCC                                           <  SRR4302533.2588957/94‑1 (MQ=60)
                                                    GTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCC                                          <  SRR4302533.5592467/94‑1 (MQ=60)
                                                     TGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCA                                         <  SRR4302533.1019639/94‑1 (MQ=60)
                                                     TGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCA                                         >  SRR4302533.1073830/1‑94 (MQ=60)
                                                     TGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCA                                         <  SRR4302533.3152634/94‑1 (MQ=60)
                                                        TGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCA                                      <  SRR4302533.232152/94‑1 (MQ=60)
                                                          CCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACT                                    >  SRR4302533.4411249/1‑94 (MQ=60)
                                                          CCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACT                                    <  SRR4302533.76563/94‑1 (MQ=60)
                                                          CCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACT                                    >  SRR4302533.3569456/1‑94 (MQ=60)
                                                          CCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACT                                    <  SRR4302533.5447926/94‑1 (MQ=60)
                                                           CTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTT                                   >  SRR4302533.4224234/1‑94 (MQ=60)
                                                             GCGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGAGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTA                                 <  SRR4302533.3398639/94‑1 (MQ=60)
                                                              CGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAC                                >  SRR4302533.1867717/1‑94 (MQ=60)
                                                              CGTTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTAC                                <  SRR4302533.3516459/94‑1 (MQ=60)
                                                                TTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCA                              >  SRR4302533.82262/1‑94 (MQ=60)
                                                                TTGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCA                              <  SRR4302533.5621083/94‑1 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                             >  SRR4302533.3379175/1‑94 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                             <  SRR4302533.1890012/94‑1 (MQ=60)
                                                                 TGCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAA                             <  SRR4302533.2531429/94‑1 (MQ=60)
                                                                  GCGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAG                            <  SRR4302533.5478095/94‑1 (MQ=60)
                                                                  GCGTAAAGGCGAAGAAGATCGCATCTTGCCAGCAGTTAATAAAGGAGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAC                            >  SRR4302533.2707073/1‑94 (MQ=60)
                                                                   CGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGC                           >  SRR4302533.1910272/1‑94 (MQ=60)
                                                                   CGTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGC                           <  SRR4302533.5169807/94‑1 (MQ=60)
                                                                    GTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCC                          <  SRR4302533.1183085/94‑1 (MQ=60)
                                                                    GTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCC                          >  SRR4302533.4028781/1‑94 (MQ=60)
                                                                    GTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCC                          <  SRR4302533.1122559/94‑1 (MQ=60)
                                                                    GTAAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCC                          <  SRR4302533.4531642/94‑1 (MQ=60)
                                                                      AAAGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGC                        >  SRR4302533.5308115/1‑94 (MQ=60)
                                                                        AGGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCA                      <  SRR4302533.5872833/94‑1 (MQ=60)
                                                                         GGCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAG                     <  SRR4302533.485822/94‑1 (MQ=60)
                                                                          GCGATGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGC                    <  SRR4302533.2297950/94‑1 (MQ=60)
                                                                              TGAAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGT                >  SRR4302533.4134918/1‑94 (MQ=60)
                                                                                AAGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTT              <  SRR4302533.6080277/94‑1 (MQ=60)
                                                                                 AGATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTC             >  SRR4302533.3381855/1‑94 (MQ=60)
                                                                                 AAATCGCATCTTGCC‑GCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA            <  SRR4302533.2674467/94‑1 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA            >  SRR4302533.575936/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA            >  SRR4302533.2298993/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA            <  SRR4302533.5632055/94‑1 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCA            >  SRR4302533.4796839/1‑94 (MQ=60)
                                                                                  GATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTGTCA            >  SRR4302533.3560965/1‑94 (MQ=60)
                                                                                   ATCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAG           >  SRR4302533.4588191/1‑94 (MQ=60)
                                                                                    TCGCATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACCCCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGT          <  SRR4302533.2860176/94‑1 (MQ=60)
                                                                                       CATCTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAA       <  SRR4302533.639076/94‑1 (MQ=60)
                                                                                          CTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCA    >  SRR4302533.5782101/1‑94 (MQ=60)
                                                                                          CTTGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCA    >  SRR4302533.890451/1‑94 (MQ=60)
                                                                                            TGCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC  <  SRR4302533.4077171/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATC  >  NC_000913/1330328‑1330513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑