Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,418	A→G	S522G (AGC→GGC)	acnA →	aconitate hydrase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,418	0	A	G	100.0%	760.0 / NA	239	S522G (AGC→GGC)	acnA	aconitate hydrase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (114/125); total (114/125)

Only 100 of 261 total aligned reads displayed.

CTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCG  >  NC_000913/1335328‑1335507

 tGGGGTTTAACCTTGTGGGATTCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCg                                                                                        >  2:6447408/1‑93 (MQ=255)

  ggggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGa                                                                                       <  1:1446296/93‑1 (MQ=255)

   gggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAt                                                                                      <  1:8262661/93‑1 (MQ=255)

   gggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAt                                                                                      <  1:673035/93‑1 (MQ=255)

    ggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGGCGAtt                                                                                     <  1:6259153/93‑1 (MQ=255)

      tttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                   <  1:1331433/93‑1 (MQ=255)

      tttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                   <  2:7512219/93‑1 (MQ=255)

       ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTaa                                                                                  <  1:907130/93‑1 (MQ=255)

       ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTaa                                                                                  >  2:5439942/1‑93 (MQ=255)

        tAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                 <  1:5707825/93‑1 (MQ=255)

        tAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                 <  1:8939651/93‑1 (MQ=255)

         acccTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAcc                                                                                <  2:2849920/91‑1 (MQ=255)

         aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAcc                                                                                <  1:1847768/93‑1 (MQ=255)

          aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCg                                                                               >  1:7278716/1‑93 (MQ=255)

           ccTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGt                                                                              >  2:7580187/1‑93 (MQ=255)

           ccTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGt                                                                              >  2:2897835/1‑93 (MQ=255)

            cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTc                                                                             <  2:952624/93‑1 (MQ=255)

             ttGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcg                                                                            >  1:2971358/1‑93 (MQ=255)

              tgtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcgg                                                                           <  2:7047048/93‑1 (MQ=255)

               gtgGGATACGGTTTTACCCCCTGTATTGGTAACTCTGGGACGCTTCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                          <  2:8371434/93‑1 (MQ=255)

               gtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAACAAGGCGATTTAACCGTcggg                                                                          >  2:916803/1‑92 (MQ=255)

               gtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                          >  1:9732612/1‑93 (MQ=255)

               gtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                          <  2:1142829/93‑1 (MQ=255)

               gtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                          <  2:2021063/93‑1 (MQ=255)

                tgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtg                                                                         <  2:1835745/93‑1 (MQ=255)

                       cGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                  >  1:2622783/1‑93 (MQ=255)

                       cGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                  >  2:6918772/1‑93 (MQ=255)

                           tGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTcc                                                              <  2:5338401/93‑1 (MQ=255)

                                caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGTCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                         >  2:9344763/1‑93 (MQ=255)

                                caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                         <  1:2551155/93‑1 (MQ=255)

                                caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                         <  1:783623/93‑1 (MQ=255)

                                   cTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCg                                                      >  2:307997/1‑93 (MQ=255)

                                    tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                     >  2:8496672/1‑93 (MQ=255)

                                    tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                     >  2:4896788/1‑93 (MQ=255)

                                    tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                     >  1:7082056/1‑93 (MQ=255)

                                    tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                     >  2:9503266/1‑93 (MQ=255)

                                      tATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                   >  2:4537144/1‑93 (MQ=255)

                                      tATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                   <  2:3290213/93‑1 (MQ=255)

                                      tATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                   >  2:5147970/1‑93 (MQ=255)

                                       aTTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                  <  2:3493610/93‑1 (MQ=255)

                                       aTTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGCCGGTGCGGTGCTGTCCGGCAACCGTCAc                                                  >  1:8677227/1‑93 (MQ=255)

                                        ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAGAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACt                                                 >  2:6001413/1‑93 (MQ=255)

                                         tGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                  >  1:1673/1‑75 (MQ=255)

                                         tGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                                <  1:4247731/93‑1 (MQ=255)

                                         tGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                                <  2:8823790/93‑1 (MQ=255)

                                          ggTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACttt                                               >  2:4171281/1‑93 (MQ=255)

                                          ggTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACttt                                               <  1:10031617/93‑1 (MQ=255)

                                           gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTg                                              >  2:1280684/1‑93 (MQ=255)

                                            tAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGa                                             <  2:4133237/93‑1 (MQ=255)

                                            tAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGa                                             <  1:4864419/93‑1 (MQ=255)

                                            tAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGa                                             <  1:818895/93‑1 (MQ=255)

                                             aaCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGaa                                            <  1:5186388/93‑1 (MQ=255)

                                              aCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAg                                           >  1:7616576/1‑93 (MQ=255)

                                               ctctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                          <  2:8291982/93‑1 (MQ=255)

                                               ctctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                          <  1:6414774/93‑1 (MQ=255)

                                               ctctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                          >  1:1996694/1‑93 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                         <  1:1117283/93‑1 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                         <  1:7463524/93‑1 (MQ=255)

                                                 ctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGcc                                        <  2:2815557/93‑1 (MQ=255)

                                                   gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCTGGCAACCGTAACTTTGAAGGCCGt                                      <  1:4503100/93‑1 (MQ=255)

                                                   gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGt                                      >  1:3025091/1‑93 (MQ=255)

                                                   gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGt                                      <  2:7830911/93‑1 (MQ=255)

                                                    ggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                     >  2:4250769/1‑93 (MQ=255)

                                                      ccGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatc                                   <  2:255354/93‑1 (MQ=255)

                                                       cGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcc                                  <  2:8744693/93‑1 (MQ=255)

                                                        gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcca                                 <  1:6540793/93‑1 (MQ=255)

                                                        ccTGCCCGATCCTATCGAAACCGCAATCAAAAAAGGCGATTTAACCGGCGGTGCGGGGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcca                                 <  2:567249/92‑1 (MQ=255)

                                                         cTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccat                                >  2:2514162/1‑93 (MQ=255)

                                                          tGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatc                               >  1:9843685/1‑93 (MQ=255)

                                                          tGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatc                               <  2:5039361/93‑1 (MQ=255)

                                                           gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTAAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                              >  1:3240258/1‑93 (MQ=255)

                                                               gATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                          <  2:6684748/93‑1 (MQ=255)

                                                               gATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGAGCTGTCCGGCAACCGCAACTTTGAAGGCCGTATCCATCCGCTg                          >  1:797298/1‑93 (MQ=255)

                                                                aTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                         >  2:8284028/1‑93 (MQ=255)

                                                                aTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                         >  1:5455231/1‑93 (MQ=255)

                                                                 tCCTATCGAAACGGCAGTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGt                        <  1:6140434/93‑1 (MQ=255)

                                                                  ccTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                       >  2:298594/1‑93 (MQ=255)

                                                                   ccaTCGAAACGGCATTCAAAAAAGACGATTTAACCGTCGGTGCGGTACTGTCCGGCAACCGTAACTTTGCAGGCCGTATCCATCCGCTGGTTa                      >  2:8555292/3‑93 (MQ=255)

                                                                   cTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTa                      >  2:3726639/1‑93 (MQ=255)

                                                                    tATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                     >  1:5775654/1‑93 (MQ=255)

                                                                     aTCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaa                    >  1:502513/1‑93 (MQ=255)

                                                                      tCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                   <  2:6790360/93‑1 (MQ=255)

                                                                        gAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaact                 >  1:9548185/1‑93 (MQ=255)

                                                                         aaaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaacta                >  2:5974721/1‑93 (MQ=255)

                                                                         aaaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaacta                <  2:147311/93‑1 (MQ=255)

                                                                          aaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaa               <  1:6762732/93‑1 (MQ=255)

                                                                            cGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact             <  1:2223873/93‑1 (MQ=255)

                                                                            cGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact             <  1:1362762/93‑1 (MQ=255)

                                                                             ggCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg            <  1:4358825/93‑1 (MQ=255)

                                                                               cAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggc          >  2:3653297/1‑93 (MQ=255)

                                                                                aaTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggct         <  2:7804380/93‑1 (MQ=255)

                                                                                 aTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg        >  2:2238170/1‑93 (MQ=255)

                                                                                 aTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg        >  2:430185/1‑93 (MQ=255)

                                                                                   cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACtggctggc      >  2:4386028/1‑93 (MQ=255)

                                                                                    aaaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc     <  2:1034319/93‑1 (MQ=255)

                                                                                     aaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt    >  1:4766716/1‑93 (MQ=255)

                                                                                     aaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt    >  2:7901586/1‑93 (MQ=255)

                                                                                      aaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc   >  1:4453238/1‑93 (MQ=255)

                                                                                      aaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc   <  2:6641383/93‑1 (MQ=255)

                                                                                       aaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcg  >  1:1621137/1‑93 (MQ=255)

CTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCG  >  NC_000913/1335328‑1335507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCG  >  NC_000913/1335325‑1335510

GAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG                                                                                              <  SRR4302540.1027474/94‑1 (MQ=60)

GAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG                                                                                              >  SRR4302540.5377723/1‑94 (MQ=60)

GAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG                                                                                              <  SRR4302540.8207504/94‑1 (MQ=60)

 AACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG                                                                                             >  SRR4302540.9566117/1‑94 (MQ=60)

    TGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGA                                                                                          >  SRR4302540.1519775/1‑94 (MQ=60)

     GGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAT                                                                                         >  SRR4302540.4315606/1‑94 (MQ=60)

     GGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAT                                                                                         <  SRR4302540.1462022/94‑1 (MQ=60)

     GGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAT                                                                                         <  SRR4302540.5785937/94‑1 (MQ=60)

      GGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATT                                                                                        <  SRR4302540.680242/94‑1 (MQ=60)

      GGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATT                                                                                        <  SRR4302540.8358678/94‑1 (MQ=60)

       GTTTTAACCTTGTGGGATACGGTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTT                                                                                       <  SRR4302540.9011109/94‑1 (MQ=60)

       GGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTT                                                                                       <  SRR4302540.1453127/94‑1 (MQ=60)

       GGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGGCGATTT                                                                                       <  SRR4302540.6328793/94‑1 (MQ=60)

         tatagCCTTGTGGGATCCGGTTGTTCCACTTGTATTGGTAACTGTGGGCCTCTTCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAA                                                                                     <  SRR4302540.1345349/89‑1 (MQ=60)

         TTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAA                                                                                     <  SRR4302540.1345351/94‑1 (MQ=60)

          TTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAC                                                                                    <  SRR4302540.1324294/94‑1 (MQ=60)

          TTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAC                                                                                    <  SRR4302540.1324295/94‑1 (MQ=60)

          TTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAC                                                                                    <  SRR4302540.916759/94‑1 (MQ=60)

           TAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACC                                                                                   <  SRR4302540.9044497/94‑1 (MQ=60)

           TAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACC                                                                                   <  SRR4302540.5770666/94‑1 (MQ=60)

            AACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCG                                                                                  <  SRR4302540.1868032/94‑1 (MQ=60)

            AACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCG                                                                                  >  SRR4302540.7359925/1‑94 (MQ=60)

              CCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAACAAGGCGATTTAACCGTC                                                                                >  SRR4302540.446999/1‑94 (MQ=60)

              CCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTC                                                                                <  SRR4302540.1372762/94‑1 (MQ=60)

               CTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCG                                                                               >  SRR4302540.3004176/1‑94 (MQ=60)

                TTGTGGGATGCGGTTGTACCTCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGG                                                                              <  SRR4302540.2188230/94‑1 (MQ=60)

                TTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGG                                                                              <  SRR4302540.2187982/94‑1 (MQ=60)

                 TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGT                                                                             >  SRR4302540.769581/1‑94 (MQ=60)

                 TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGT                                                                             >  SRR4302540.1658889/1‑94 (MQ=60)

                 TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGT                                                                             >  SRR4302540.9846897/1‑94 (MQ=60)

                  GTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTG                                                                            <  SRR4302540.4177788/94‑1 (MQ=60)

                   TGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGC                                                                           >  SRR4302540.3274049/1‑94 (MQ=60)

                   TGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGC                                                                           >  SRR4302540.457187/1‑94 (MQ=60)

                    GGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCG                                                                          >  SRR4302540.3526636/1‑94 (MQ=60)

                    GGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCG                                                                          <  SRR4302540.8519406/94‑1 (MQ=60)

                      GATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGT                                                                        >  SRR4302540.1061457/1‑94 (MQ=60)

                      GATACGGCTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGT                                                                        <  SRR4302540.7948985/94‑1 (MQ=60)

                         ACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCT                                                                     >  SRR4302540.9978623/1‑94 (MQ=60)

                         ACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCT                                                                     >  SRR4302540.2651143/1‑94 (MQ=60)

                           GGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGT                                                                   >  SRR4302540.1509328/1‑94 (MQ=60)

                            GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTC                                                                  >  SRR4302540.6258630/1‑94 (MQ=60)

                                 ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCA                                                             <  SRR4302540.6809577/94‑1 (MQ=60)

                                   CACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC                                                           <  SRR4302540.791956/94‑1 (MQ=60)

                                   CACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC                                                           <  SRR4302540.2578654/94‑1 (MQ=60)

                                   CACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC                                                           >  SRR4302540.1416603/1‑94 (MQ=60)

                                    ACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACC                                                          >  SRR4302540.9513995/1‑94 (MQ=60)

                                     CCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCG                                                         >  SRR4302540.5049873/1‑94 (MQ=60)

                                     CCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCG                                                         >  SRR4302540.3041105/1‑94 (MQ=60)

                                     CCTGTATTGATAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCG                                                         <  SRR4302540.4710787/94‑1 (MQ=60)

                                      CTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGT                                                        >  SRR4302540.7160875/1‑94 (MQ=60)

                                        GTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAA                                                      >  SRR4302540.6427818/1‑94 (MQ=60)

                                         TATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC                                                     <  SRR4302540.8231158/94‑1 (MQ=60)

                                         TATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGCCGGTGCGGTGCTGTCCGGCAACCGTCAC                                                     >  SRR4302540.8778004/1‑94 (MQ=60)

                                          tatggttacccctggccccctccccATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACT                                                    <  SRR4302540.2928169/69‑1 (MQ=60)

                                           TTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGaagcgctagatcggaag                                                   >  SRR4302540.1688/1‑77 (MQ=60)

                                            TGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTT                                                  <  SRR4302540.4293945/94‑1 (MQ=60)

                                            TGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTT                                                  <  SRR4302540.5305888/94‑1 (MQ=60)

                                             GGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTG                                                 <  SRR4302540.10148308/94‑1 (MQ=60)

                                              GTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGA                                                <  SRR4302540.8088037/94‑1 (MQ=60)

                                               TAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAA                                               <  SRR4302540.4916593/94‑1 (MQ=60)

                                               TAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAA                                               <  SRR4302540.827598/94‑1 (MQ=60)

                                                AACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAG                                              <  SRR4302540.5242852/94‑1 (MQ=60)

                                                AACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAG                                              >  SRR4302540.7702203/1‑94 (MQ=60)

                                                 ACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGG                                             >  SRR4302540.2018325/1‑94 (MQ=60)

                                                  CTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGC                                            <  SRR4302540.6486157/94‑1 (MQ=60)

                                                   TCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           <  SRR4302540.5185865/94‑1 (MQ=60)

                                                   TCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           <  SRR4302540.7546756/94‑1 (MQ=60)

                                                   TCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           >  SRR4302540.4877081/1‑94 (MQ=60)

                                                   TCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           >  SRR4302540.8885419/1‑94 (MQ=60)

                                                   TCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           <  SRR4302540.1128868/94‑1 (MQ=60)

                                                   TATGGGCCGCTGCCCGCTCCTATCGAAACGGCACTCAAAAGCGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCC                                           <  SRR4302540.7802966/94‑1 (MQ=60)

                                                    CTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCG                                          >  SRR4302540.8052600/1‑94 (MQ=60)

                                                     TGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGT                                         >  SRR4302540.3058320/1‑94 (MQ=60)

                                                      GGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCTGGCAACCGTAACTTTGAAGGCCGTA                                        <  SRR4302540.4551915/94‑1 (MQ=60)

                                                          CGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCA                                    >  SRR4302540.9873736/1‑94 (MQ=60)

                                                          CGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCA                                    >  SRR4302540.9873735/1‑94 (MQ=60)

                                                          CGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCA                                    <  SRR4302540.2870600/94‑1 (MQ=60)

                                                           GCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCAT                                   <  SRR4302540.6613374/94‑1 (MQ=60)

                                                            CTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATC                                  >  SRR4302540.9958755/1‑94 (MQ=60)

                                                            CTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATC                                  >  SRR4302540.4511044/1‑94 (MQ=60)

                                                            CTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGAGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATC                                  >  SRR4302540.915879/1‑94 (MQ=60)

                                                             TGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCC                                 <  SRR4302540.7354440/94‑1 (MQ=60)

                                                             TGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCC                                 <  SRR4302540.5810863/94‑1 (MQ=60)

                                                             TGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCC                                 >  SRR4302540.6765607/1‑94 (MQ=60)

                                                             TGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTAAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCC                                 >  SRR4302540.3275514/1‑94 (MQ=60)

                                                              GCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCG                                <  SRR4302540.6494059/94‑1 (MQ=60)

                                                              GCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCG                                <  SRR4302540.6752957/94‑1 (MQ=60)

                                                                CCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCT                              <  SRR4302540.4903091/94‑1 (MQ=60)

                                                                 CGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGAGCTGTCCGGCAACCGCAACTTTGAAGGCCGTATCCATCCGCTG                             >  SRR4302540.805767/1‑94 (MQ=60)

                                                                  GATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGG                            >  SRR4302540.6963695/1‑94 (MQ=60)

                                                                  GATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGG                            >  SRR4302540.5515276/1‑94 (MQ=60)

                                                                  GATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGG                            >  SRR4302540.52988/1‑94 (MQ=60)

                                                                   ATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGT                           >  SRR4302540.5641620/1‑94 (MQ=60)

                                                                   ATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGT                           <  SRR4302540.8310106/94‑1 (MQ=60)

                                                                    TCCTATCGAAACGGCAGTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTT                          <  SRR4302540.6208820/94‑1 (MQ=60)

                                                                    TCCTATCGAAACGACAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTT                          <  SRR4302540.6208823/94‑1 (MQ=60)

                                                                     CCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTA                         >  SRR4302540.7515956/1‑94 (MQ=60)

                                                                      CTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAA                        >  SRR4302540.9837641/1‑94 (MQ=60)

                                                                      CTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAA                        >  SRR4302540.5839457/1‑94 (MQ=60)

                                                                       TATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTCACTTTGAAGGCCGTATCCATCCGCTGGTTAAA                       >  SRR4302540.2280702/1‑94 (MQ=60)

                                                                       TATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAA                       >  SRR4302540.507900/1‑94 (MQ=60)

                                                                         tacAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAAC                     <  SRR4302540.1818136/91‑1 (MQ=60)

                                                                         TCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAAC                     <  SRR4302540.4446874/94‑1 (MQ=60)

                                                                         TCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAAC                     <  SRR4302540.1818137/94‑1 (MQ=60)

                                                                          CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACT                    >  SRR4302540.9661137/1‑94 (MQ=60)

                                                                          CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACT                    >  SRR4302540.840204/1‑94 (MQ=60)

                                                                          CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACT                    <  SRR4302540.7183425/94‑1 (MQ=60)

                                                                             AACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCTGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAC                 <  SRR4302540.2380349/94‑1 (MQ=60)

                                                                             AACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAC                 <  SRR4302540.5080648/94‑1 (MQ=60)

                                                                             AACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAC                 <  SRR4302540.6837469/94‑1 (MQ=60)

                                                                               CGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTG               <  SRR4302540.2247843/94‑1 (MQ=60)

                                                                               CGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTG               <  SRR4302540.1377161/94‑1 (MQ=60)

                                                                               CGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTG               <  SRR4302540.2579564/94‑1 (MQ=60)

                                                                               CGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTG               >  SRR4302540.6905806/1‑94 (MQ=60)

                                                                                GGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGG              <  SRR4302540.3073903/94‑1 (MQ=60)

                                                                                GGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGG              <  SRR4302540.4406230/94‑1 (MQ=60)

                                                                                   AATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTG           <  SRR4302540.6474001/94‑1 (MQ=60)

                                                                                    ATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGG          <  SRR4302540.9254279/94‑1 (MQ=60)

                                                                                      CAAAAAAGGCGATTTAACCGTCGGTGCGGTGCCGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCC        <  SRR4302540.2004313/94‑1 (MQ=60)

                                                                                       AAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCT       >  SRR4302540.7653578/1‑94 (MQ=60)

                                                                                       AAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCT       >  SRR4302540.4817621/1‑94 (MQ=60)

                                                                                        AAAAAGGCGATTTAACCGTCGTTGCGGTGCTGTACGGAAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTTACTGGCCTGCCTC      >  SRR4302540.8022530/1‑94 (MQ=60)

                                                                                        AAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTC      >  SRR4302540.5884592/1‑94 (MQ=60)

                                                                                        AAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTC      >  SRR4302540.4501513/1‑94 (MQ=60)

                                                                                         AAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCG     >  SRR4302540.1638908/1‑94 (MQ=60)

                                                                                          AAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGC    <  SRR4302540.2939064/94‑1 (MQ=60)

                                                                                           AAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC   >  SRR4302540.7540369/1‑94 (MQ=60)

                                                                                           AAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC   >  SRR4302540.1634451/1‑94 (MQ=60)

                                                                                           AAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC   <  SRR4302540.8786427/94‑1 (MQ=60)

                                                                                            AGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGTTGGCCTCGCCG  >  SRR4302540.8222583/1‑94 (MQ=60)

                                                                                            AGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCG  <  SRR4302540.6811216/94‑1 (MQ=60)

                                                                                            AGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCG  >  SRR4302540.6087670/1‑94 (MQ=60)

                                                                                            AGGCGATTTAACCGTCAGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCG  <  SRR4302540.4963290/94‑1 (MQ=60)

GAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCG  >  NC_000913/1335325‑1335510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`