Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,337,333 A→G intergenic (‑149/‑21) ribA ← / → pgpB GTP cyclohydrolase II/non‑essential phosphatidylglycerophosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,337,3330AG100.0% 620.6 / NA 192intergenic (‑149/‑21)ribA/pgpBGTP cyclohydrolase II/non‑essential phosphatidylglycerophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (104/88);  total (104/88)

Only 100 of 196 total aligned reads displayed.

TTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGG  >  NC_000913/1337243‑1337422
                                                                                          |                                                                                         
ttCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCt                                                                                         >  1:1159367/1‑93 (MQ=255)
ttCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCt                                                                                         <  1:2256266/93‑1 (MQ=255)
  cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTa                                                                                       <  1:1194266/93‑1 (MQ=255)
  cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTa                                                                                       >  2:2979094/1‑93 (MQ=255)
  cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTa                                                                                       <  1:4810739/93‑1 (MQ=255)
  cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTa                                                                                       >  1:4608641/1‑93 (MQ=255)
  cACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTa                                                                                       >  1:4344132/1‑93 (MQ=255)
    ccTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATc                                                                                     <  2:1182074/93‑1 (MQ=255)
    ccTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATc                                                                                     <  2:1334456/93‑1 (MQ=255)
        aTCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCaaaa                                                                                 >  2:5651336/1‑93 (MQ=255)
         tCACGACGGGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCaaaaa                                                                                <  1:5195881/93‑1 (MQ=255)
         tCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCaaaaa                                                                                <  1:2631908/93‑1 (MQ=255)
         tCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCaaaaa                                                                                <  1:4969438/93‑1 (MQ=255)
          cACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAg                                                                               >  1:3050719/1‑93 (MQ=255)
           acgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAgg                                                                              <  1:1993007/93‑1 (MQ=255)
            cgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGga                                                                             <  2:1706687/93‑1 (MQ=255)
            cgacgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGga                                                                             <  1:2854355/93‑1 (MQ=255)
              acgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGgaga                                                                           >  1:599773/1‑93 (MQ=255)
              acgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGgaga                                                                           <  1:3326750/93‑1 (MQ=255)
               cgcgTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGagag                                                                          <  2:2698404/93‑1 (MQ=255)
                 cgTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGc                                                                        >  1:27313/1‑93 (MQ=255)
                 cgTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGc                                                                        >  1:4487004/1‑93 (MQ=255)
                 cgTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGc                                                                        >  2:4337359/1‑93 (MQ=255)
                  gTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGcc                                                                       >  1:3899495/1‑93 (MQ=255)
                  gTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGcc                                                                       >  1:5092801/1‑93 (MQ=255)
                   tCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCa                                                                      <  1:3106620/93‑1 (MQ=255)
                   tCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTATGGGGCTTATCAAAAAGGAGAGGCCa                                                                      >  2:5089812/1‑93 (MQ=255)
                    cGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAt                                                                     <  2:2887977/93‑1 (MQ=255)
                    cGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAt                                                                     >  2:6141500/1‑93 (MQ=255)
                     gATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATg                                                                    <  2:3991185/93‑1 (MQ=255)
                     gATCGTGCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATg                                                                    <  1:508177/93‑1 (MQ=255)
                       tCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCg                                                                  >  2:1822774/1‑93 (MQ=255)
                          ttCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTc                                                               >  2:2833200/1‑93 (MQ=255)
                          ttCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTc                                                               >  1:162965/1‑93 (MQ=255)
                           tCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCg                                                              >  2:5180495/1‑93 (MQ=255)
                             aCGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAt                                                                     <  2:823196/84‑1 (MQ=255)
                             aCGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAt                                                                     >  1:823196/1‑84 (MQ=255)
                             aCGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGAt                                                            <  2:2289110/93‑1 (MQ=255)
                              cGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGAtt                                                           <  1:477219/93‑1 (MQ=255)
                              cGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGAtt                                                           <  1:2387973/93‑1 (MQ=255)
                              cGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGAtt                                                           <  2:6290861/93‑1 (MQ=255)
                              cGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGAtt                                                           <  1:5556172/93‑1 (MQ=255)
                                gAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGc                                                         >  1:293147/1‑93 (MQ=255)
                                gAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGc                                                         >  1:5445011/1‑93 (MQ=255)
                                 aaTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGcc                                                        >  1:5730500/1‑93 (MQ=255)
                                  aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCa                                                       <  2:3200095/93‑1 (MQ=255)
                                  aTGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCa                                                       <  2:5021463/93‑1 (MQ=255)
                                    ggCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGa                                                     >  1:623562/1‑93 (MQ=255)
                                     gCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGAc                                                    >  1:1893950/1‑93 (MQ=255)
                                        caAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTa                                                 <  2:2076674/93‑1 (MQ=255)
                                         aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAc                                                >  2:3808001/1‑93 (MQ=255)
                                         aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAc                                                <  2:408204/93‑1 (MQ=255)
                                          aaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAcc                                               >  1:4091464/1‑93 (MQ=255)
                                            tCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc                                             >  1:12787/1‑93 (MQ=255)
                                            tCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGc                                             >  2:4397688/1‑93 (MQ=255)
                                             cTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGTGGCCATGCGTTCGATTGCCAGACGTACCGCa                                            >  2:1165452/1‑93 (MQ=255)
                                             cTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCa                                            <  2:5729304/93‑1 (MQ=255)
                                              tGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAg                                           >  2:3338765/1‑93 (MQ=255)
                                                tCAATTTTTCGTGGAGCTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTg                                         <  2:2225286/93‑1 (MQ=255)
                                                tCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTg                                         >  2:1661804/1‑93 (MQ=255)
                                                 cAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTgg                                        <  1:367982/93‑1 (MQ=255)
                                                 cAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTgg                                        <  1:4779040/93‑1 (MQ=255)
                                                    tttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAg                                     <  2:796346/93‑1 (MQ=255)
                                                    tttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAg                                     <  1:6067469/93‑1 (MQ=255)
                                                     ttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCATACGTACCGCAGTGGGAGc                                    >  1:1549346/1‑93 (MQ=255)
                                                     ttttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGc                                    <  2:5436315/93‑1 (MQ=255)
                                                      tttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCt                                   >  1:5642147/1‑93 (MQ=255)
                                                       ttCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTg                                  >  2:3854144/1‑93 (MQ=255)
                                                          cTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCAc                               >  2:1878951/1‑93 (MQ=255)
                                                          cTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCAc                               >  2:4058549/1‑93 (MQ=255)
                                                           tGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACt                              >  1:4716056/1‑93 (MQ=255)
                                                           tGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTA‑CGCAGTGGGAGCTGCACTa                             >  1:125136/1‑93 (MQ=255)
                                                            ggAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTa                             <  2:3540851/93‑1 (MQ=255)
                                                            ggAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTa                             >  2:2635160/1‑93 (MQ=255)
                                                             gAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTAt                            >  2:3653480/1‑93 (MQ=255)
                                                             gAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTAt                            >  2:1401063/1‑93 (MQ=255)
                                                             gAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTAt                            <  2:4622444/93‑1 (MQ=255)
                                                              aaCTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTAtt                           <  2:5225346/93‑1 (MQ=255)
                                                                 tGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCt                        >  2:4122595/1‑93 (MQ=255)
                                                                     gTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTc                    >  1:2006107/1‑93 (MQ=255)
                                                                      ttttCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCa                   >  1:3526667/1‑93 (MQ=255)
                                                                       tttCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCAt                  <  1:708942/93‑1 (MQ=255)
                                                                        ttCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATg                 >  2:4890315/1‑93 (MQ=255)
                                                                         tCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGc                >  2:3583709/1‑93 (MQ=255)
                                                                         tCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGc                <  2:4162962/93‑1 (MQ=255)
                                                                          cAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGcc               <  1:4753207/93‑1 (MQ=255)
                                                                          cAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGcc               <  1:2574596/93‑1 (MQ=255)
                                                                           aGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCa              >  1:3030647/1‑93 (MQ=255)
                                                                           aGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCa              >  2:5551623/1‑93 (MQ=255)
                                                                           aGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCa              >  2:596431/1‑93 (MQ=255)
                                                                               aTGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAg          >  2:5738041/1‑93 (MQ=255)
                                                                               aTGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAg          <  2:1024545/93‑1 (MQ=255)
                                                                                tGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGc         <  2:299117/93‑1 (MQ=255)
                                                                                 gATTGTGGGGCTTAGAAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGcc        <  1:182797/93‑1 (MQ=255)
                                                                                   ttGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGt      >  2:244275/1‑93 (MQ=255)
                                                                                   ttGTGGGGCTAATCAAAAAGGAGAGCCCTTCCGTTCAATTCCAAGACTTCCCG‑AATGGGAGGTGGCATATTGCTTGTCATGCCAGTAGCCGTa     <  2:898830/93‑1 (MQ=255)
                                                                                    tgtgGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTa     <  2:4001217/93‑1 (MQ=255)
                                                                                    tgtgGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTa     <  2:2006440/93‑1 (MQ=255)
                                                                                     gtgGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTAt    <  1:5324313/93‑1 (MQ=255)
                                                                                      tgGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATg   <  1:4317962/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGG  >  NC_000913/1337243‑1337422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGAT  >  NC_000913/1337242‑1337424
                                                                                           |                                                                                           
GTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCT                                                                                           >  SRR4302533.1166325/1‑94 (MQ=60)
GTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCT                                                                                           <  SRR4302533.3829291/94‑1 (MQ=60)
 TTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTT                                                                                          <  SRR4302533.2270225/94‑1 (MQ=60)
 TTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTT                                                                                          <  SRR4302533.444394/94‑1 (MQ=60)
  TCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTA                                                                                         >  SRR4302533.2952379/1‑94 (MQ=60)
  TCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTA                                                                                         >  SRR4302533.4636552/1‑94 (MQ=60)
  TCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTA                                                                                         >  SRR4302533.4370312/1‑94 (MQ=60)
   CACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTAT                                                                                        <  SRR4302533.1201445/94‑1 (MQ=60)
   CACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTAT                                                                                        <  SRR4302533.4839897/94‑1 (MQ=60)
       TAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAA                                                                                    >  SRR4302533.2317150/1‑94 (MQ=60)
       TAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAA                                                                                    <  SRR4302533.3794913/94‑1 (MQ=60)
          TCACGACGGGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAG                                                                                 <  SRR4302533.5227216/94‑1 (MQ=60)
          TCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAG                                                                                 <  SRR4302533.2648080/94‑1 (MQ=60)
          TCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAG                                                                                 >  SRR4302533.3069393/1‑94 (MQ=60)
          TCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAG                                                                                 >  SRR4302533.157295/1‑94 (MQ=60)
          TCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAG                                                                                 <  SRR4302533.4999567/94‑1 (MQ=60)
            ACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGA                                                                               <  SRR4302533.2005230/94‑1 (MQ=60)
             CGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAG                                                                              <  SRR4302533.126201/94‑1 (MQ=60)
             CGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAG                                                                              <  SRR4302533.2871919/94‑1 (MQ=60)
              GACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGA                                                                             >  SRR4302533.603302/1‑94 (MQ=60)
              GACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGA                                                                             <  SRR4302533.2872374/94‑1 (MQ=60)
               ACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAG                                                                            <  SRR4302533.3347305/94‑1 (MQ=60)
                 GCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGC                                                                          >  SRR4302533.1857673/1‑94 (MQ=60)
                 GCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGC                                                                          >  SRR4302533.4514170/1‑94 (MQ=60)
                 GCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGC                                                                          >  SRR4302533.27497/1‑94 (MQ=60)
                  CGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCC                                                                         >  SRR4302533.5123568/1‑94 (MQ=60)
                  CGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCC                                                                         >  SRR4302533.3923290/1‑94 (MQ=60)
                   GTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCA                                                                        >  SRR4302533.4523351/1‑94 (MQ=60)
                    TCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAT                                                                       <  SRR4302533.3125712/94‑1 (MQ=60)
                    TCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCAT                                                                       >  SRR4302533.1229831/1‑94 (MQ=60)
                      GATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGC                                                                     >  SRR4302533.4808705/1‑94 (MQ=60)
                      GATCGTGCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGC                                                                     <  SRR4302533.511194/94‑1 (MQ=60)
                          GTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTC                                                                 >  SRR4302533.164002/1‑94 (MQ=60)
                          GTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTC                                                                 <  SRR4302533.35239/94‑1 (MQ=60)
                             CACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGatacagca                                                              >  SRR4302533.828026/1‑86 (MQ=60)
                               CGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTG                                                            <  SRR4302533.1308261/94‑1 (MQ=60)
                               CGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTG                                                            <  SRR4302533.5589750/94‑1 (MQ=60)
                               CGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTG                                                            <  SRR4302533.480067/94‑1 (MQ=60)
                               CGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTG                                                            <  SRR4302533.2402725/94‑1 (MQ=60)
                               CGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTG                                                            <  SRR4302533.3660457/94‑1 (MQ=60)
                                GGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGC                                                           >  SRR4302533.294938/1‑94 (MQ=60)
                                GGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGC                                                           >  SRR4302533.5477818/1‑94 (MQ=60)
                                 GAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCC                                                          >  SRR4302533.5765101/1‑94 (MQ=60)
                                 GAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCC                                                          >  SRR4302533.5499471/1‑94 (MQ=60)
                                   ATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAG                                                        >  SRR4302533.4993763/1‑94 (MQ=60)
                                   ATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAG                                                        >  SRR4302533.4671064/1‑94 (MQ=60)
                                    TGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGA                                                       >  SRR4302533.627224/1‑94 (MQ=60)
                                     GGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGAC                                                      >  SRR4302533.1905512/1‑94 (MQ=60)
                                       CACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGT                                                    >  SRR4302533.5962996/1‑94 (MQ=60)
                                         CAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAC                                                  >  SRR4302533.704848/1‑94 (MQ=60)
                                         CAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAC                                                  <  SRR4302533.1828009/94‑1 (MQ=60)
                                         CAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAC                                                  >  SRR4302533.3343998/1‑94 (MQ=60)
                                          AAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACC                                                 >  SRR4302533.4116369/1‑94 (MQ=60)
                                           AATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCG                                                <  SRR4302533.2867408/94‑1 (MQ=60)
                                           AATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCG                                                >  SRR4302533.615333/1‑94 (MQ=60)
                                            ATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGC                                               >  SRR4302533.12883/1‑94 (MQ=60)
                                              CTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAG                                             <  SRR4302533.3946666/94‑1 (MQ=60)
                                               TGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGT                                            <  SRR4302533.5112101/94‑1 (MQ=60)
                                               TGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGT                                            >  SRR4302533.4761022/1‑94 (MQ=60)
                                                GTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTG                                           >  SRR4302533.5724570/1‑94 (MQ=60)
                                                  CACTTTTTCCTGGAACTGGCTTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGG                                         <  SRR4302533.5454285/94‑1 (MQ=60)
                                                  CAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGG                                         <  SRR4302533.370208/94‑1 (MQ=60)
                                                  CAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGG                                         <  SRR4302533.4807988/94‑1 (MQ=60)
                                                    ATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAG                                       >  SRR4302533.2728020/1‑94 (MQ=60)
                                                    ATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAG                                       >  SRR4302533.3055204/1‑94 (MQ=60)
                                                     TTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCATACGTACCGCAGTGGGAGC                                      >  SRR4302533.1558685/1‑94 (MQ=60)
                                                     TTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGC                                      <  SRR4302533.6104185/94‑1 (MQ=60)
                                                      TTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCT                                     >  SRR4302533.5676249/1‑94 (MQ=60)
                                                          CCTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGAACCGCAGTGGGAGCTGCAC                                 >  SRR4302533.2418990/1‑94 (MQ=60)
                                                           CTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACT                                >  SRR4302533.4744643/1‑94 (MQ=60)
                                                           CTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACT                                >  SRR4302533.3118511/1‑94 (MQ=60)
                                                           CTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACT                                >  SRR4302533.520469/1‑94 (MQ=60)
                                                           CTGGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTA‑CGCAGTGGGAGCTGCACTA                               >  SRR4302533.125921/1‑94 (MQ=60)
                                                             GGAACTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTAT                              <  SRR4302533.1003130/94‑1 (MQ=60)
                                                                 CTGGCGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCT                          <  SRR4302533.2012809/94‑1 (MQ=60)
                                                                     CGTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTC                      >  SRR4302533.2018462/1‑94 (MQ=60)
                                                                      GTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCT                     >  SRR4302533.1927062/1‑94 (MQ=60)
                                                                      GTTTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCA                     >  SRR4302533.3548403/1‑94 (MQ=60)
                                                                        TTTCAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATG                   <  SRR4302533.713130/94‑1 (MQ=60)
                                                                           CAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCA                <  SRR4302533.4782022/94‑1 (MQ=60)
                                                                           CAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCA                <  SRR4302533.2590434/94‑1 (MQ=60)
                                                                           CAGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCA                >  SRR4302533.3049193/1‑94 (MQ=60)
                                                                            AGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAG               >  SRR4302533.2641572/1‑94 (MQ=60)
                                                                            AGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAG               <  SRR4302533.4787171/94‑1 (MQ=60)
                                                                            AGTTATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAG               >  SRR4302533.2069316/1‑94 (MQ=60)
                                                                               TATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAG            <  SRR4302533.5120565/94‑1 (MQ=60)
                                                                                ATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGC           >  SRR4302533.5337040/1‑94 (MQ=60)
                                                                                ATGATTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGC           <  SRR4302533.5648872/94‑1 (MQ=60)
                                                                                  GATTGTGGGGCTTAGAAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCG         <  SRR4302533.183933/94‑1 (MQ=60)
                                                                                    TTGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTA       >  SRR4302533.432750/1‑94 (MQ=60)
                                                                                     TGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTAT      <  SRR4302533.4802874/94‑1 (MQ=60)
                                                                                     TGTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTAA      >  SRR4302533.6085558/1‑94 (MQ=60)
                                                                                      GTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATG     >  SRR4302533.800948/1‑94 (MQ=60)
                                                                                      GTGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATG     <  SRR4302533.5356222/94‑1 (MQ=60)
                                                                                       TGGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGG    <  SRR4302533.4343966/94‑1 (MQ=60)
                                                                                        GGGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGA   >  SRR4302533.383140/1‑94 (MQ=60)
                                                                                         GGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGAT  <  SRR4302533.3232324/94‑1 (MQ=60)
                                                                                         GGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGAT  >  SRR4302533.4551144/1‑94 (MQ=60)
                                                                                         GGGCTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGAT  <  SRR4302533.6286791/94‑1 (MQ=60)
                                                                                           |                                                                                           
GTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGAT  >  NC_000913/1337242‑1337424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑