Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,445,885 A→G I115V (ATT→GTT)  feaB → phenylacetaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,445,8850AG98.6% 652.4 / ‑1.0 207I115V (ATT→GTT) feaBphenylacetaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (105/99);  total (106/101)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 217 total aligned reads displayed.

CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGAC  >  NC_000913/1445795‑1445974
                                                                                          |                                                                                         
cGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGtt                                                                                         >  1:6632698/1‑93 (MQ=255)
cGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGtt                                                                                         >  2:5390255/1‑93 (MQ=255)
 gTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGttt                                                                                        >  1:9250442/1‑93 (MQ=255)
  tATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTc                                                                                       <  1:3638096/93‑1 (MQ=255)
  tATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTc                                                                                       >  2:3222212/1‑93 (MQ=255)
  tATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGAACAGTGAGGAGCTGGCGCAACTGGCAACCCTGGAGCAAGGCAAGCCAATTACCGTTTc                                                                                       >  1:2664935/1‑93 (MQ=255)
    ttCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTccc                                                                                     >  2:4316017/1‑93 (MQ=255)
    ttCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTccc                                                                                     >  1:526680/1‑93 (MQ=255)
         cTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCt                                                                                >  1:690913/1‑93 (MQ=255)
          tACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGGTTCCCGTGCtt                                                                               >  2:271057/1‑93 (MQ=255)
           cccttttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGACGCAACTGGAACCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCttt                                                                              <  1:3401048/90‑1 (MQ=255)
           aCGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCttt                                                                              <  2:1546837/93‑1 (MQ=255)
            cGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCtttt                                                                             >  1:4311577/1‑93 (MQ=255)
             gTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTg                                                                            >  1:3598502/1‑93 (MQ=255)
              ttttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGa                                                                           <  2:390572/93‑1 (MQ=255)
              ttttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGa                                                                           <  1:4751198/93‑1 (MQ=255)
               tttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGaa                                                                          <  1:5867791/93‑1 (MQ=255)
               tttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGaa                                                                          <  1:1965726/93‑1 (MQ=255)
               tttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGaa                                                                          <  1:8038634/93‑1 (MQ=255)
               tttGCTGATCTGGTGGAACAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGaa                                                                          <  1:8963947/93‑1 (MQ=255)
                ttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAg                                                                         <  1:8774152/93‑1 (MQ=255)
                  gCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTg                                                                       <  2:4495026/93‑1 (MQ=255)
                    tGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTggg                                                                     >  1:9502628/1‑93 (MQ=255)
                    tGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTggg                                                                     >  2:6447046/1‑93 (MQ=255)
                     gATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGc                                                                    <  2:7736226/93‑1 (MQ=255)
                      aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCt                                                                   <  2:239329/93‑1 (MQ=255)
                      aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCt                                                                   <  2:8583985/93‑1 (MQ=255)
                      aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCt                                                                   >  1:6489041/1‑93 (MQ=255)
                       tCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTg                                                                  >  2:4776934/1‑93 (MQ=255)
                         tggtgaAGCAGCACAGTGAGGAGCGGGAGCAGCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTa                                                                <  1:264104/93‑1 (MQ=255)
                          ggtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTAc                                                               <  2:5944819/93‑1 (MQ=255)
                           gtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACg                                                              >  1:6221023/1‑93 (MQ=255)
                            tggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGc                                                             <  1:1598375/93‑1 (MQ=255)
                            tggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGc                                                             <  1:7571216/93‑1 (MQ=255)
                             ggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCt                                                            >  1:5209555/1‑93 (MQ=255)
                             ggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCt                                                            >  1:9071037/1‑93 (MQ=255)
                               agcagcACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGa                                                          <  1:2187026/93‑1 (MQ=255)
                               agcagcACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGa                                                          <  1:8671580/93‑1 (MQ=255)
                                 cagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAc                                                        <  2:6943433/93‑1 (MQ=255)
                                 cagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAc                                                        <  1:1233455/93‑1 (MQ=255)
                                 cagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAc                                                        <  2:5767915/93‑1 (MQ=255)
                                 cagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAc                                                        <  1:4016213/93‑1 (MQ=255)
                                  agcaCAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACt                                                       >  2:2569076/1‑93 (MQ=255)
                                     acaGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGa                                                    <  1:1464439/93‑1 (MQ=255)
                                      caGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGCACTGGAt                                                   >  2:9652324/1‑93 (MQ=255)
                                       aGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATg                                                  <  1:4053284/93‑1 (MQ=255)
                                        gTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGc                                                 <  2:4058147/93‑1 (MQ=255)
                                            ggagCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTta                                             <  2:5562836/93‑1 (MQ=255)
                                            ggagCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTta                                             <  2:1037794/93‑1 (MQ=255)
                                             gagCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtat                                            <  2:9060064/93‑1 (MQ=255)
                                             gagCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtat                                            <  1:2749871/93‑1 (MQ=255)
                                              agCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtata                                           >  2:786343/1‑93 (MQ=255)
                                               gCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAc                                          >  2:5651237/1‑93 (MQ=255)
                                               gCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAc                                          <  2:2679631/93‑1 (MQ=255)
                                               gCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAc                                          <  2:6127004/93‑1 (MQ=255)
                                                 tGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccg                                        >  1:2468501/1‑93 (MQ=255)
                                                   gcgcAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCTTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgcc                                      <  1:4356970/93‑1 (MQ=255)
                                                       aaCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGt                                  <  1:2323509/93‑1 (MQ=255)
                                                        aCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGtt                                 >  2:8005974/1‑93 (MQ=255)
                                                        aCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGtt                                 >  1:5042842/1‑93 (MQ=255)
                                                        aCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGtt                                 <  1:3113641/93‑1 (MQ=255)
                                                         cTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTa                                <  1:7250869/93‑1 (MQ=255)
                                                          tGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTaa                               <  2:3833021/93‑1 (MQ=255)
                                                          tGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTaa                               >  1:6339531/1‑93 (MQ=255)
                                                           ggAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAc                              >  1:4243987/1‑93 (MQ=255)
                                                           ggAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAc                              <  2:1315220/93‑1 (MQ=255)
                                                           ggAAACCCTGGAGCAAGGCAAGTAAATTGCCGTTTCACGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAc                              <  2:3784164/93‑1 (MQ=255)
                                                             aaaCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGa                            <  2:190918/93‑1 (MQ=255)
                                                              aaCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGAc                           >  1:988263/1‑93 (MQ=255)
                                                              aaCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGAc                           >  2:8485501/1‑93 (MQ=255)
                                                                cccTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCa                         >  2:6192652/1‑93 (MQ=255)
                                                                 ccTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCaa                        <  1:3149077/93‑1 (MQ=255)
                                                                  cTGGAGCAAGGCAAGCCAAGTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATCGGTTATAAGGCCGGGTTAACGACCaaa                       <  2:8225498/93‑1 (MQ=255)
                                                                   tGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCaaaa                      <  1:879292/93‑1 (MQ=255)
                                                                   tGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCaaaa                      >  2:1954512/1‑93 (MQ=255)
                                                                    ggAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAt                     <  2:7290102/93‑1 (MQ=255)
                                                                      aGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGAGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATcg                   <  1:8857943/93‑1 (MQ=255)
                                                                         aaggcaagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCgg                >  2:8825760/1‑93 (MQ=255)
                                                                          aggcaagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCggg               >  1:7444726/1‑93 (MQ=255)
                                                                           ggcaagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGt              >  2:294146/1‑93 (MQ=255)
                                                                            gcaagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTa             >  1:2659996/1‑93 (MQ=255)
                                                                             caagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaa            <  2:4626265/93‑1 (MQ=255)
                                                                              aagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaaa           >  1:8231010/1‑93 (MQ=255)
                                                                              aagTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaaa           <  1:7060788/93‑1 (MQ=255)
                                                                               agTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaaaa          >  1:5980270/1‑93 (MQ=255)
                                                                               agTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaaaa          <  1:7423283/93‑1 (MQ=255)
                                                                               agTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTaaaa          >  2:2793089/1‑93 (MQ=255)
                                                                                gTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAc         <  2:6398276/93‑1 (MQ=255)
                                                                                 tCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACg        >  2:4749959/1‑93 (MQ=255)
                                                                                 tCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACg        >  2:762713/1‑93 (MQ=255)
                                                                                  taaTTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGc       <  1:3320833/92‑1 (MQ=255)
                                                                                    aTTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTg     <  2:6311072/93‑1 (MQ=255)
                                                                                    aTTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTg     <  1:4561212/93‑1 (MQ=255)
                                                                                     ttGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTgg    >  2:1045380/1‑93 (MQ=255)
                                                                                     ttGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTgg    >  1:9571319/1‑93 (MQ=255)
                                                                                      tGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGa   <  1:1873817/93‑1 (MQ=255)
                                                                                      tGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGa   >  2:9260680/1‑93 (MQ=255)
                                                                                      tGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGa   <  2:7061019/93‑1 (MQ=255)
                                                                                       gCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGAc  <  1:9737339/93‑1 (MQ=255)
                                                                                       gCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGAc  >  2:3401362/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGAC  >  NC_000913/1445795‑1445974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTTGT  >  NC_000913/1445792‑1445978
                                                                                             |                                                                                             
GAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCG                                                                                               >  SRR4302540.502916/1‑94 (MQ=60)
 AACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGT                                                                                              >  SRR4302540.5093028/1‑94 (MQ=60)
 AACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGT                                                                                              >  SRR4302540.593530/1‑94 (MQ=60)
 AACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGT                                                                                              >  SRR4302540.8986257/1‑94 (MQ=60)
  ACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTT                                                                                             >  SRR4302540.6706111/1‑94 (MQ=60)
   CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTT                                                                                            >  SRR4302540.9361177/1‑94 (MQ=60)
    GTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTC                                                                                           >  SRR4302540.9398323/1‑94 (MQ=60)
    GTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGAACAGTGAGGAGCTGGCGCAACTGGCAACCCTGGAGCAAGGCAAGCCAATTACCGTTTC                                                                                           >  SRR4302540.2693829/1‑94 (MQ=60)
     TATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCC                                                                                          >  SRR4302540.7874988/1‑94 (MQ=60)
     TATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCC                                                                                          <  SRR4302540.3677927/94‑1 (MQ=60)
     TATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCC                                                                                          <  SRR4302540.3677926/94‑1 (MQ=60)
      ATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGGCGTTTCCC                                                                                         <  SRR4302540.8982582/94‑1 (MQ=60)
      ATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCC                                                                                         >  SRR4302540.532371/1‑94 (MQ=60)
           GCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCT                                                                                    >  SRR4302540.698331/1‑94 (MQ=60)
            CTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTT                                                                                   <  SRR4302540.5262781/94‑1 (MQ=60)
            CTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTT                                                                                   >  SRR4302540.1379475/1‑94 (MQ=60)
             TACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTT                                                                                  <  SRR4302540.6243774/94‑1 (MQ=60)
             TACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTT                                                                                  >  SRR4302540.8183737/1‑94 (MQ=60)
              cccTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGACGCAACTGGAACCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTT                                                                                 <  SRR4302540.3438125/91‑1 (MQ=60)
              ACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTT                                                                                 >  SRR4302540.4358515/1‑94 (MQ=60)
              ACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTACCGTGCTTTT                                                                                 >  SRR4302540.4358516/1‑94 (MQ=60)
              ACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAACAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTT                                                                                 >  SRR4302540.9845253/1‑94 (MQ=60)
               CGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTG                                                                                >  SRR4302540.3637818/1‑94 (MQ=60)
               CGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTG                                                                                <  SRR4302540.8841116/94‑1 (MQ=60)
                GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGA                                                                               >  SRR4302540.7139550/1‑94 (MQ=60)
                GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGA                                                                               >  SRR4302540.9171240/1‑94 (MQ=60)
                GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGA                                                                               >  SRR4302540.9626142/1‑94 (MQ=60)
                GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACACTGGAGCAAGGCAAGTCAATTACCGTTTCCCGTGCTTTTGA                                                                               >  SRR4302540.4089858/1‑94 (MQ=60)
                 TTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAA                                                                              <  SRR4302540.4801901/94‑1 (MQ=60)
                 TTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAA                                                                              >  SRR4302540.7088050/1‑94 (MQ=60)
                  TTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAG                                                                             <  SRR4302540.8132056/94‑1 (MQ=60)
                  TTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAG                                                                             <  SRR4302540.5932664/94‑1 (MQ=60)
                  TTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAG                                                                             <  SRR4302540.1987075/94‑1 (MQ=60)
                  TTTGCTGATCTGGTGGAACAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAG                                                                             <  SRR4302540.9070112/94‑1 (MQ=60)
                   TTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGT                                                                            <  SRR4302540.7563178/94‑1 (MQ=60)
                   TTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGT                                                                            <  SRR4302540.8875799/94‑1 (MQ=60)
                   TTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGT                                                                            >  SRR4302540.3978350/1‑94 (MQ=60)
                    TGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTG                                                                           >  SRR4302540.2679851/1‑94 (MQ=60)
                      GTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGG                                                                         <  SRR4302540.3449076/94‑1 (MQ=60)
                      CTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGG                                                                         >  SRR4302540.9615227/1‑94 (MQ=60)
                        GATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCT                                                                       >  SRR4302540.6561203/1‑94 (MQ=60)
                         ATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTtggcgt                                                                      >  SRR4302540.1936659/1‑88 (MQ=60)
                            TGGTGAAGCAGCACAGTGAGGAGCGGGAGCAGCTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTAC                                                                   <  SRR4302540.266687/94‑1 (MQ=60)
                             GGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACG                                                                  >  SRR4302540.6290226/1‑94 (MQ=60)
                               TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                                                >  SRR4302540.5266310/1‑94 (MQ=60)
                               TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                                                <  SRR4302540.7656151/94‑1 (MQ=60)
                               TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                                                >  SRR4302540.5266311/1‑94 (MQ=60)
                               TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                                                >  SRR4302540.9179974/1‑94 (MQ=60)
                               TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                                                <  SRR4302540.1615849/94‑1 (MQ=60)
                                  AGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAA                                                             <  SRR4302540.8772309/94‑1 (MQ=60)
                                  AGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAA                                                             <  SRR4302540.2210636/94‑1 (MQ=60)
                                  AGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAA                                                             <  SRR4302540.9874397/94‑1 (MQ=60)
                                  AGCAGCACAGTGAGGAGCGGCCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAA                                                             <  SRR4302540.8772308/94‑1 (MQ=60)
                                    CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                                           >  SRR4302540.6344461/1‑94 (MQ=60)
                                    CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                                           <  SRR4302540.1246050/94‑1 (MQ=60)
                                    CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                                           >  SRR4302540.3345730/1‑94 (MQ=60)
                                    CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                                           <  SRR4302540.4059473/94‑1 (MQ=60)
                                     AGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTG                                                          <  SRR4302540.3437786/94‑1 (MQ=60)
                                      GCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGTAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGG                                                         <  SRR4302540.2745853/94‑1 (MQ=60)
                                      GCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGG                                                         <  SRR4302540.6602212/94‑1 (MQ=60)
                                        ACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGAT                                                       >  SRR4302540.4708335/1‑94 (MQ=60)
                                        ACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGAT                                                       <  SRR4302540.1480411/94‑1 (MQ=60)
                                         CAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATG                                                      <  SRR4302540.2108119/94‑1 (MQ=60)
                                          AGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGC                                                     <  SRR4302540.2334275/94‑1 (MQ=60)
                                          AGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGC                                                     <  SRR4302540.4096986/94‑1 (MQ=60)
                                                GAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATA                                               <  SRR4302540.2779586/94‑1 (MQ=60)
                                                  GCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGGCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACC                                             >  SRR4302540.1488187/1‑94 (MQ=60)
                                                  GCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACC                                             <  SRR4302540.8896209/94‑1 (MQ=60)
                                                   ctcggcgtactggaagcgCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCG                                            <  SRR4302540.3179747/76‑1 (MQ=60)
                                                   CTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCG                                            >  SRR4302540.2495068/1‑94 (MQ=60)
                                                    TGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGC                                           >  SRR4302540.6542863/1‑94 (MQ=60)
                                                    TGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTAGATGCGTTATAgctc                                           >  SRR4302540.9246768/1‑90 (MQ=60)
                                                     GGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCC                                          <  SRR4302540.315025/94‑1 (MQ=60)
                                                      GCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCTTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCG                                         <  SRR4302540.4404358/94‑1 (MQ=60)
                                                        GCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGG                                       >  SRR4302540.2943111/1‑94 (MQ=60)
                                                        GCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGG                                       >  SRR4302540.5025887/1‑94 (MQ=60)
                                                        GCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGG                                       <  SRR4302540.7374187/94‑1 (MQ=60)
                                                          AACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTT                                     <  SRR4302540.2348528/94‑1 (MQ=60)
                                                          AACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTT                                     >  SRR4302540.5097391/1‑94 (MQ=60)
                                                           ACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTA                                    <  SRR4302540.8879467/94‑1 (MQ=60)
                                                           ACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTA                                    <  SRR4302540.3147691/94‑1 (MQ=60)
                                                           ACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTA                                    <  SRR4302540.2249609/94‑1 (MQ=60)
                                                            CTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAA                                   <  SRR4302540.7331775/94‑1 (MQ=60)
                                                            CTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAA                                   >  SRR4302540.6410133/1‑94 (MQ=60)
                                                             TGGAAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAC                                  >  SRR4302540.4290164/1‑94 (MQ=60)
                                                              ggaaactcgtGAGCAAGGCAAGTCAAGTGCCGTTTCCCGTGCTTTTGAAGGGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACG                                 <  SRR4302540.2464914/84‑1 (MQ=60)
                                                                AAACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGAC                               >  SRR4302540.998357/1‑94 (MQ=60)
                                                                 AGCCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACC                              <  SRR4302540.7508774/94‑1 (MQ=60)
                                                                 AACCCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACC                              <  SRR4302540.1657217/94‑1 (MQ=60)
                                                                  ACCCTGGAGCAAGGCAAGTCAATTGGCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA                             >  SRR4302540.4327394/1‑94 (MQ=60)
                                                                    CCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAA                           >  SRR4302540.3857235/1‑94 (MQ=60)
                                                                    CCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAA                           <  SRR4302540.3183375/94‑1 (MQ=60)
                                                                    CCTGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAA                           >  SRR4302540.8634717/1‑94 (MQ=60)
                                                                      TGGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAT                         <  SRR4302540.888619/94‑1 (MQ=60)
                                                                      GGGAGCAAGGCAAGTGAATTGCCGTTTCCTGTGCGTTTGAAGTGGGCTGTACGCTGAGCTTGATGCGTTATACCGCCGGGTTAACGACCAAAAT                         <  SRR4302540.6017763/94‑1 (MQ=60)
                                                                       GGAGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATC                        <  SRR4302540.5936718/94‑1 (MQ=60)
                                                                         AGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGC                      <  SRR4302540.10049354/94‑1 (MQ=60)
                                                                         AGCAAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGAGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGC                      <  SRR4302540.8960438/94‑1 (MQ=60)
                                                                           CAAGGCAAGTCAATTGGCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGG                    >  SRR4302540.6277288/1‑94 (MQ=60)
                                                                            AAGGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGG                   >  SRR4302540.7527674/1‑94 (MQ=60)
                                                                              GGCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTA                 >  SRR4302540.2688854/1‑94 (MQ=60)
                                                                               GGAAGTCAATTGCCATTTCCAGTGCGTTTGAAGTGGGCTGTAAGCTGAACTGAATGGGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAA                <  SRR4302540.9205611/94‑1 (MQ=60)
                                                                               GCAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAA                <  SRR4302540.9350645/94‑1 (MQ=60)
                                                                                CAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAA               >  SRR4302540.4762418/1‑94 (MQ=60)
                                                                                CAAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAA               >  SRR4302540.8326687/1‑94 (MQ=60)
                                                                                 AAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAA              >  SRR4302540.6046481/1‑94 (MQ=60)
                                                                                 AAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAA              <  SRR4302540.7139317/94‑1 (MQ=60)
                                                                                 AAGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAA              <  SRR4302540.7139316/94‑1 (MQ=60)
                                                                                  AGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAC             <  SRR4302540.7505975/94‑1 (MQ=60)
                                                                                  AGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAC             <  SRR4302540.2693145/94‑1 (MQ=60)
                                                                                  AGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAC             <  SRR4302540.4163614/94‑1 (MQ=60)
                                                                                  AGTCAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAC             >  SRR4302540.5329672/1‑94 (MQ=60)
                                                                                  AGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAAC             <  SRR4302540.4518195/94‑1 (MQ=60)
                                                                                   GTCAATTGGCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACG            >  SRR4302540.2917538/1‑94 (MQ=60)
                                                                                     TAATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCT          <  SRR4302540.3357264/94‑1 (MQ=60)
                                                                                      AATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTG         >  SRR4302540.6750869/1‑94 (MQ=60)
                                                                                       ATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGG        <  SRR4302540.4610494/94‑1 (MQ=60)
                                                                                       ATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGG        >  SRR4302540.2581152/1‑94 (MQ=60)
                                                                                       ATTGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGG        >  SRR4302540.9684394/1‑94 (MQ=60)
                                                                                         TGCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGAC      <  SRR4302540.1894344/94‑1 (MQ=60)
                                                                                          GCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACT     >  SRR4302540.3416849/1‑94 (MQ=60)
                                                                                          GCCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACT     <  SRR4302540.9851656/94‑1 (MQ=60)
                                                                                           CCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTT    >  SRR4302540.9113238/1‑94 (MQ=60)
                                                                                           CCGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTG    >  SRR4302540.3397427/1‑94 (MQ=60)
                                                                                            CGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTTG   >  SRR4302540.2785313/1‑94 (MQ=60)
                                                                                            CGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTTG   <  SRR4302540.8085970/94‑1 (MQ=60)
                                                                                            CGTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGGTAACGACCAAAATCGCGGGTAAAACGCTGGACTTG   >  SRR4302540.1978754/1‑94 (MQ=60)
                                                                                             GTTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTTGT  >  SRR4302540.2423383/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGTAAAACGCTGGACTTGT  >  NC_000913/1445792‑1445978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑