Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,510,339	T→C	V221A (GTG→GCG)	b1440 →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,510,339	0	T	C	99.5%	572.1 / ‑5.9	183	V221A (GTG→GCG)	b1440	putative transport protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (88/93); total (90/93)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 189 total aligned reads displayed.

TTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGT  >  NC_000913/1510249‑1510427

ttGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGgcgc                                                                                        >  1:5657460/1‑93 (MQ=255)

   cGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGa                                                                                     <  2:6320393/93‑1 (MQ=255)

   cGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGa                                                                                     <  2:1211431/93‑1 (MQ=255)

   cGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGa                                                                                     >  1:3846024/1‑93 (MQ=255)

    ggACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGaa                                                                                    >  2:3450560/1‑93 (MQ=255)

    ggACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGaa                                                                                    <  1:7021458/93‑1 (MQ=255)

     gACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGaaa                                                                                   >  2:5947834/1‑93 (MQ=255)

      aCGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAg                                                                                  <  1:4998585/93‑1 (MQ=255)

      aCGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAg                                                                                  <  1:4591762/93‑1 (MQ=255)

       cgcgGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGt                                                                                 <  2:1081396/93‑1 (MQ=255)

       cGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGt                                                                                 >  1:2691562/1‑93 (MQ=255)

       cGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAAAAGTACCAGGCGGCGCTGAAAGt                                                                                 <  1:1748817/93‑1 (MQ=255)

         cTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgc                                                                               <  1:2530444/93‑1 (MQ=255)

          tGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgct                                                                              >  1:608431/1‑93 (MQ=255)

          tGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgct                                                                              <  1:4488090/93‑1 (MQ=255)

          tGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgct                                                                              >  2:1582051/1‑93 (MQ=255)

           gCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgctg                                                                             >  2:9903939/1‑93 (MQ=255)

           gCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgctg                                                                             >  1:4916580/1‑93 (MQ=255)

              ttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgc                                                                          >  1:9072253/1‑93 (MQ=255)

              ttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgc                                                                          <  1:1107421/93‑1 (MQ=255)

              ttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgc                                                                          <  2:8089048/93‑1 (MQ=255)

              ttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgc                                                                          >  2:7458099/1‑93 (MQ=255)

                gttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgcgc                                                                        >  2:2245591/1‑93 (MQ=255)

                     ttAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAac                                                                   >  2:6400844/1‑93 (MQ=255)

                      tAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAaca                                                                  <  1:9137221/93‑1 (MQ=255)

                       aaaGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAacac                                                                 <  2:6242123/93‑1 (MQ=255)

                        aaGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAAcaca                                                                <  1:940492/93‑1 (MQ=255)

                          gCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGt                                                              <  2:6616846/93‑1 (MQ=255)

                           ccgcTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGtt                                                             <  2:7253448/90‑1 (MQ=255)

                           ccACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGtt                                                             <  1:7665150/93‑1 (MQ=255)

                            cACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGttt                                                            >  1:9912660/1‑93 (MQ=255)

                            cACTCA‑CCGCAGTTGGGCAACAGGCATCCGTATCAACTCCCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTg                                                           <  1:6820479/93‑1 (MQ=255)

                              cTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGa                                                          >  2:4064724/1‑93 (MQ=255)

                              cTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGa                                                          <  1:5775412/93‑1 (MQ=255)

                               tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                         <  2:6881435/93‑1 (MQ=255)

                               tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                         <  2:4152991/93‑1 (MQ=255)

                               tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                         >  2:8721857/1‑93 (MQ=255)

                                cAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATc                                                        >  1:3586972/1‑93 (MQ=255)

                                cAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATc                                                        <  2:9526213/93‑1 (MQ=255)

                                 aGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATcc                                                       <  2:5770080/93‑1 (MQ=255)

                                  gCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCa                                                      <  2:2993436/93‑1 (MQ=255)

                                   ccGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                                                     <  1:5140537/93‑1 (MQ=255)

                                    cGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATc                                                    >  1:9244730/1‑93 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   >  2:2812443/1‑93 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   <  2:1940765/93‑1 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   <  1:7408854/93‑1 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   >  2:8554693/1‑93 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   >  1:3083101/1‑93 (MQ=255)

                                     gCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                   >  1:5530559/1‑93 (MQ=255)

                                         ttGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTAc                                               >  1:4071263/1‑93 (MQ=255)

                                         ttGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTAc                                               >  2:1724415/1‑93 (MQ=255)

                                          tGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACt                                              <  1:4161111/93‑1 (MQ=255)

                                           gggCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTg                                             >  2:4185373/1‑93 (MQ=255)

                                           gggCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTg                                             >  1:9550578/1‑93 (MQ=255)

                                              cATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCa                                          <  1:9493446/93‑1 (MQ=255)

                                               aTCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCAt                                         <  1:5293686/93‑1 (MQ=255)

                                                tCAGCGTTCCGTTTCAACTTTTCGTAGTTCAGTTTCTGGCGGCGCTGTAAGTGCCGCGCGCTCAACACAGTTTGATCTATCGCTATAGGCagg                                        >  1:8992808/1‑91 (MQ=255)

                                                tCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATg                                        >  1:7260363/1‑93 (MQ=255)

                                                tCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATg                                        >  2:6798305/1‑93 (MQ=255)

                                                 cAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGa                                       >  1:9226807/1‑93 (MQ=255)

                                                  aGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGac                                      <  2:9917570/93‑1 (MQ=255)

                                                  aGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGac                                      >  1:5446779/1‑93 (MQ=255)

                                                   gCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca                                     >  1:8749571/1‑93 (MQ=255)

                                                   gCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca                                     >  1:2134694/1‑93 (MQ=255)

                                                      aTCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTTATCCATCCCTACTGGCATGACACtc                                  >  1:5127233/1‑92 (MQ=255)

                                                      aTCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTa                                  >  2:4909003/1‑93 (MQ=255)

                                                       tCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAc                                 <  1:2329200/93‑1 (MQ=255)

                                                       tCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAc                                 <  1:9386380/93‑1 (MQ=255)

                                                        ccGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAcc                                <  2:2128012/93‑1 (MQ=255)

                                                        ccGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAcc                                <  1:1647004/93‑1 (MQ=255)

                                                         cGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCg                               <  2:5387449/93‑1 (MQ=255)

                                                          gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCgc                              >  2:7283197/1‑92 (MQ=255)

                                                          gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGt                              >  2:5090647/1‑93 (MQ=255)

                                                            aTCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGc                            <  1:4925499/93‑1 (MQ=255)

                                                               aaCTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCaaa                         <  2:3085294/93‑1 (MQ=255)

                                                               aaCTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCaaa                         <  1:7170777/93‑1 (MQ=255)

                                                               aaCTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCaaa                         <  1:7018748/93‑1 (MQ=255)

                                                                aCTCACCTAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAt                        <  2:7632988/93‑1 (MQ=255)

                                                                  tCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGa                      >  1:5978645/1‑93 (MQ=255)

                                                                   cACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAg                     >  2:7400214/1‑93 (MQ=255)

                                                                    aCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGc                    <  1:5183811/93‑1 (MQ=255)

                                                                    aCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGc                    <  1:689924/93‑1 (MQ=255)

                                                                      cGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGTGa                  >  2:2449995/1‑93 (MQ=255)

                                                                      cGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGa                  >  2:7105026/1‑93 (MQ=255)

                                                                      cGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGa                  <  1:9690535/93‑1 (MQ=255)

                                                                        aagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGGGCGAtt                <  2:9759921/93‑1 (MQ=255)

                                                                        aagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAtt                <  1:1075767/93‑1 (MQ=255)

                                                                             cAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaa           >  1:817761/1‑93 (MQ=255)

                                                                             cAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaa           <  1:9196479/93‑1 (MQ=255)

                                                                               gTACCAGGCGGCGCTGAAAGTGCTGCGGGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaaaa         <  1:5726534/93‑1 (MQ=255)

                                                                               gTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaaaa         >  1:2272242/1‑93 (MQ=255)

                                                                               gTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaaaa         >  1:2071844/1‑93 (MQ=255)

                                                                                tACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAAc        <  2:3905808/93‑1 (MQ=255)

                                                                                   cAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAg     <  1:6293818/93‑1 (MQ=255)

                                                                                   cAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAg     >  1:4811279/1‑93 (MQ=255)

                                                                                   cAGGCGGCGCTGAAAGGTCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAg     <  2:2205488/93‑1 (MQ=255)

                                                                                    aGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACTCTACCGTGCAAATGAGCGATTTCAAAAACGAgg    >  2:2329183/1‑93 (MQ=255)

                                                                                    aGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAgg    >  2:1636835/1‑93 (MQ=255)

                                                                                     ggcggcGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAggg   <  1:6600364/93‑1 (MQ=255)

                                                                                      gcggcgCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGgt  <  2:3291573/93‑1 (MQ=255)

TTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGT  >  NC_000913/1510249‑1510427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  NC_000913/1510246‑1510432

ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGC                                                                                               >  SRR4302540.4988555/1‑94 (MQ=60)

ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGC                                                                                               >  SRR4302540.392901/1‑94 (MQ=60)

 TATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCG                                                                                              >  SRR4302540.9841666/1‑94 (MQ=60)

  ATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGC                                                                                             >  SRR4302540.5719754/1‑94 (MQ=60)

     GCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGA                                                                                          >  SRR4302540.3887849/1‑94 (MQ=60)

       GGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAA                                                                                        <  SRR4302540.7099445/94‑1 (MQ=60)

         ACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGT                                                                                      <  SRR4302540.5052706/94‑1 (MQ=60)

         ACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGT                                                                                      <  SRR4302540.4641179/94‑1 (MQ=60)

         ACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGT                                                                                      >  SRR4302540.2720637/1‑94 (MQ=60)

          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     <  SRR4302540.3687716/94‑1 (MQ=60)

          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     >  SRR4302540.8129102/1‑94 (MQ=60)

          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     >  SRR4302540.8495325/1‑94 (MQ=60)

          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAAAAGTACCAGGCGGCGCTGAAAGTG                                                                                     <  SRR4302540.1767759/94‑1 (MQ=60)

           GCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGC                                                                                    >  SRR4302540.4981695/1‑94 (MQ=60)

            CTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCT                                                                                   <  SRR4302540.2557750/94‑1 (MQ=60)

            CTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCT                                                                                   >  SRR4302540.614936/1‑94 (MQ=60)

            CTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCT                                                                                   <  SRR4302540.7248899/94‑1 (MQ=60)

             TGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTG                                                                                  >  SRR4302540.4969527/1‑94 (MQ=60)

             TGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTG                                                                                  >  SRR4302540.2586/1‑94 (MQ=60)

             TGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTG                                                                                  <  SRR4302540.4536763/94‑1 (MQ=60)

              GCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGC                                                                                 <  SRR4302540.8937631/94‑1 (MQ=60)

               TGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCG                                                                                >  SRR4302540.4007752/1‑94 (MQ=60)

               CGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCG                                                                                >  SRR4302540.4202444/1‑94 (MQ=60)

                GTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGC                                                                               >  SRR4302540.9181205/1‑94 (MQ=60)

                 TTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCG                                                                              <  SRR4302540.1118858/94‑1 (MQ=60)

                 TTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCG                                                                              <  SRR4302540.9148798/94‑1 (MQ=60)

                 TTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCG                                                                              <  SRR4302540.2232376/94‑1 (MQ=60)

                      CGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAA                                                                         >  SRR4302540.615121/1‑94 (MQ=60)

                       GTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAAC                                                                        <  SRR4302540.925508/94‑1 (MQ=60)

                       GTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAAC                                                                        >  SRR4302540.3461365/1‑94 (MQ=60)

                         TAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACAC                                                                      <  SRR4302540.5681225/94‑1 (MQ=60)

                         TAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACAC                                                                      <  SRR4302540.9247017/94‑1 (MQ=60)

                           AAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAG                                                                    <  SRR4302540.950322/94‑1 (MQ=60)

                             GCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTT                                                                  <  SRR4302540.2036507/94‑1 (MQ=60)

                             GCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTT                                                                  <  SRR4302540.175324/94‑1 (MQ=60)

                              CCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTT                                                                 <  SRR4302540.7751501/94‑1 (MQ=60)

                              CCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTT                                                                 >  SRR4302540.10028435/1‑94 (MQ=60)

                               CACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTG                                                                <  SRR4302540.705262/94‑1 (MQ=60)

                               CACTCA‑CCGCAGTTGGGCAACAGGCATCCGTATCAACTCCCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGA                                                               <  SRR4302540.6895983/94‑1 (MQ=60)

                                ACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGA                                                               <  SRR4302540.6895984/94‑1 (MQ=60)

                                ACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGAGCTGAAAGTGCTGCGCGCTCAACACAGTTTGA                                                               >  SRR4302540.9337444/1‑94 (MQ=60)

                                 CTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAT                                                              <  SRR4302540.5839213/94‑1 (MQ=60)

                                 CTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAT                                                              >  SRR4302540.7484242/1‑94 (MQ=60)

                                  TCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATC                                                             >  SRR4302540.3626148/1‑94 (MQ=60)

                                    AGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCA                                                           >  SRR4302540.9511433/1‑94 (MQ=60)

                                      CCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATC                                                         >  SRR4302540.9355434/1‑94 (MQ=60)

                                      CCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATC                                                         <  SRR4302540.5196221/94‑1 (MQ=60)

                                       CGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCG                                                        <  SRR4302540.6024963/94‑1 (MQ=60)

                                       CGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCG                                                        <  SRR4302540.6556484/94‑1 (MQ=60)

                                       CGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCG                                                        >  SRR4302540.5591469/1‑94 (MQ=60)

                                       CGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCG                                                        >  SRR4302540.5591468/1‑94 (MQ=60)

                                       CGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCG                                                        >  SRR4302540.3116931/1‑94 (MQ=60)

                                        GCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGC                                                       <  SRR4302540.7491383/94‑1 (MQ=60)

                                           GTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTAC                                                    >  SRR4302540.4115151/1‑94 (MQ=60)

                                            TTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACT                                                   <  SRR4302540.4619529/94‑1 (MQ=60)

                                             TGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTG                                                  >  SRR4302540.9663551/1‑94 (MQ=60)

                                             TGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTG                                                  >  SRR4302540.9663550/1‑94 (MQ=60)

                                             TGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTG                                                  <  SRR4302540.4206211/94‑1 (MQ=60)

                                                 CATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCAT                                              <  SRR4302540.9605969/94‑1 (MQ=60)

                                                 CATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCAT                                              <  SRR4302540.5829868/94‑1 (MQ=60)

                                                  ATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATG                                             <  SRR4302540.5351570/94‑1 (MQ=60)

                                                  ATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATG                                             >  SRR4302540.9322884/1‑94 (MQ=60)

                                                  ATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATG                                             >  SRR4302540.7341376/1‑94 (MQ=60)

                                                  ATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATG                                             >  SRR4302540.6777368/1‑94 (MQ=60)

                                                   TCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGA                                            >  SRR4302540.9337432/1‑94 (MQ=60)

                                                    CAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGAC                                           >  SRR4302540.5506717/1‑94 (MQ=60)

                                                     AGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACA                                          >  SRR4302540.8851100/1‑94 (MQ=60)

                                                     AGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACA                                          >  SRR4302540.2157607/1‑94 (MQ=60)

                                                      GCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACAC                                         <  SRR4302540.9396016/94‑1 (MQ=60)

                                                        GATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTTATCCATCCCTACTGGCATGACACTC                                       >  SRR4302540.5182763/1‑94 (MQ=60)

                                                        GATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTA                                       >  SRR4302540.5182764/1‑94 (MQ=60)

                                                        GATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTA                                       >  SRR4302540.6073872/1‑94 (MQ=60)

                                                          TCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACC                                     <  SRR4302540.5481319/94‑1 (MQ=60)

                                                          TCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACC                                     <  SRR4302540.9498100/94‑1 (MQ=60)

                                                          TCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACC                                     <  SRR4302540.2354286/94‑1 (MQ=60)

                                                           CCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCG                                    <  SRR4302540.1664978/94‑1 (MQ=60)

                                                            CGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGT                                   >  SRR4302540.9550219/1‑94 (MQ=60)

                                                             GTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTG                                  >  SRR4302540.521909/1‑94 (MQ=60)

                                                               ATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCA                                >  SRR4302540.4687517/1‑94 (MQ=60)

                                                               ATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCA                                <  SRR4302540.4978583/94‑1 (MQ=60)

                                                               ATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCA                                >  SRR4302540.7725872/1‑94 (MQ=60)

                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302540.7096703/94‑1 (MQ=60)

                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302540.7250712/94‑1 (MQ=60)

                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302540.7250711/94‑1 (MQ=60)

                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302540.9728817/94‑1 (MQ=60)

                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302540.2102007/94‑1 (MQ=60)

                                                                    CTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGA                           >  SRR4302540.6044830/1‑94 (MQ=60)

                                                                      CACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGC                         <  SRR4302540.4255640/94‑1 (MQ=60)

                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        <  SRR4302540.5240243/94‑1 (MQ=60)

                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        <  SRR4302540.2336656/94‑1 (MQ=60)

                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        >  SRR4302540.2533731/1‑94 (MQ=60)

                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        <  SRR4302540.697328/94‑1 (MQ=60)

                                                                         CGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAT                      <  SRR4302540.9804514/94‑1 (MQ=60)

                                                                          GAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATT                     <  SRR4302540.1590878/94‑1 (MQ=60)

                                                                           AAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTT                    >  SRR4302540.5094923/1‑94 (MQ=60)

                                                                           AAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTT                    <  SRR4302540.1086763/94‑1 (MQ=60)

                                                                            AGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTC                   >  SRR4302540.5325499/1‑94 (MQ=60)

                                                                               ACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAA                >  SRR4302540.826450/1‑94 (MQ=60)

                                                                                CAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAA               <  SRR4302540.9306795/94‑1 (MQ=60)

                                                                                 AGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAA              >  SRR4302540.2296627/1‑94 (MQ=60)

                                                                                 AGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAA              >  SRR4302540.2094123/1‑94 (MQ=60)

                                                                                  GTACCAGGCGGCGCTGAAAGTGCTGCGGGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAAC             <  SRR4302540.5789591/94‑1 (MQ=60)

                                                                                     CCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAG          >  SRR4302540.4862777/1‑94 (MQ=60)

                                                                                     CCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAG          >  SRR4302540.4862778/1‑94 (MQ=60)

                                                                                      CAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGG         <  SRR4302540.6363893/94‑1 (MQ=60)

                                                                                        GGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGT       <  SRR4302540.6673454/94‑1 (MQ=60)

                                                                                          CGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGT     >  SRR4302540.8866810/1‑94 (MQ=60)

                                                                                           GGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTG    >  SRR4302540.3520676/1‑94 (MQ=60)

                                                                                           GGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTG    >  SRR4302540.4657390/1‑94 (MQ=60)

                                                                                            GCGCTGAAAGTGCTGCGCGCTNAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGG   <  SRR4302540.1781563/94‑1 (MQ=60)

                                                                                            GCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGG   >  SRR4302540.373021/1‑94 (MQ=60)

                                                                                             CGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  SRR4302540.6119243/1‑94 (MQ=60)

                                                                                             CGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGGGTGGT  >  SRR4302540.643721/1‑94 (MQ=60)

ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  NC_000913/1510246‑1510432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`