Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,510,339 T→C V221A (GTG→GCG)  b1440 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,510,3390TC99.4% 516.0 / NA 168V221A (GTG→GCG) b1440putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (80/86);  total (81/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 173 total aligned reads displayed.

TGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTG  >  NC_000913/1510250‑1510428
                                                                                         |                                                                                         
tGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCt                                                                                        >  2:2599736/1‑93 (MQ=255)
 gCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTg                                                                                       <  1:1061930/93‑1 (MQ=255)
 gCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTg                                                                                       >  1:353058/1‑93 (MQ=255)
    gtcGCTGCGTTGTTCCTTAAAACCGCCCAGGCGAAGTTGGGCATCGGCGATCCGTATCACCTCACCGAAGAGCAGTACCAGGCGGTGCTGaaa                                                                                    <  2:12188/91‑1 (MQ=255)
    gaggCTGCGTTGTTCGTTAAAGCCTCTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAGCTCACCGAAGAACAGTACCAGGCGGCGCTGaaa                                                                                    <  2:1722096/90‑1 (MQ=255)
    gACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGaaa                                                                                    >  1:622047/1‑93 (MQ=255)
    gACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGaaa                                                                                    <  1:2436503/93‑1 (MQ=255)
    gACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGaaa                                                                                    <  2:473244/93‑1 (MQ=255)
      cGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGt                                                                                  <  1:4180156/93‑1 (MQ=255)
      cGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGt                                                                                  <  2:3047841/93‑1 (MQ=255)
       gCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtg                                                                                 >  1:6205614/1‑93 (MQ=255)
       gCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtg                                                                                 >  1:1033313/1‑93 (MQ=255)
       gCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATACGTATCAACTCACCGAAGAACAGTACCAGCCGGCGCTGAAAGtg                                                                                 >  2:653971/1‑93 (MQ=255)
        cTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgc                                                                                >  1:2204652/1‑93 (MQ=255)
        cTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgc                                                                                >  1:46531/1‑93 (MQ=255)
          gCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGtgctg                                                                              >  2:5646696/1‑93 (MQ=255)
            gttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcg                                                                            <  1:5019522/93‑1 (MQ=255)
            gttgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcg                                                                            >  2:34634/1‑93 (MQ=255)
              tgttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgcg                                                                          >  2:5916181/1‑93 (MQ=255)
               gttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgcgc                                                                         >  1:719733/1‑93 (MQ=255)
               gttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgcgc                                                                         <  2:5463940/93‑1 (MQ=255)
               gttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTgcgcgc                                                                         >  1:2496572/1‑93 (MQ=255)
                ttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCt                                                                        <  1:6275676/93‑1 (MQ=255)
                ttCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCt                                                                        >  1:4497233/1‑93 (MQ=255)
                  cGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCa                                                                      <  2:1870724/93‑1 (MQ=255)
                   gTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCaa                                                                     >  2:4349276/1‑93 (MQ=255)
                     tAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAaca                                                                   >  1:3379184/1‑93 (MQ=255)
                     tAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAaca                                                                   <  1:1770375/93‑1 (MQ=255)
                     tAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAaca                                                                   >  2:1333274/1‑93 (MQ=255)
                      aaaGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAacac                                                                  <  1:3829934/93‑1 (MQ=255)
                         gCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGt                                                               <  2:5258636/93‑1 (MQ=255)
                          ccACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGtt                                                              >  1:1470558/1‑93 (MQ=255)
                           cACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGttt                                                             <  1:2004418/93‑1 (MQ=255)
                           cACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGttt                                                             <  1:603218/93‑1 (MQ=255)
                            aCTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCCACACAGTTTg                                                            >  2:4995040/1‑93 (MQ=255)
                            aCTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTg                                                            >  2:1762335/1‑93 (MQ=255)
                              tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                          >  1:496911/1‑93 (MQ=255)
                              tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                          <  2:3788511/93‑1 (MQ=255)
                              tCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAt                                                          >  2:2967856/1‑93 (MQ=255)
                                  ccGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                                                      <  2:1963362/93‑1 (MQ=255)
                                   cGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATc                                                     >  2:1820775/1‑93 (MQ=255)
                                    gCAGTTGGGCATAAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCg                                                    >  2:3855542/1‑93 (MQ=255)
                                     cAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGc                                                   <  2:617045/93‑1 (MQ=255)
                                     cAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGc                                                   >  2:726388/1‑93 (MQ=255)
                                      aGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCt                                                  >  1:1481460/1‑93 (MQ=255)
                                       gTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa                                                 >  2:1469198/1‑93 (MQ=255)
                                       gTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa                                                 <  2:4917626/93‑1 (MQ=255)
                                       gTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGAACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa                                                 <  2:1317505/93‑1 (MQ=255)
                                       gTTGGGCATCAGCGATCCGGATCAACTCACCGAAGAACAGAACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGCTTGATGCATCGCTa                                                 >  2:170162/1‑93 (MQ=255)
                                           ggCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg                                             <  2:4811105/93‑1 (MQ=255)
                                              aTCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCAt                                          >  1:715161/1‑93 (MQ=255)
                                                  gCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca                                      >  2:1495719/1‑93 (MQ=255)
                                                  gCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca                                      >  2:4664744/1‑93 (MQ=255)
                                                  gCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca                                      >  1:3403983/1‑93 (MQ=255)
                                                   cGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGacac                                     <  2:2830233/93‑1 (MQ=255)
                                                   cGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGacac                                     <  2:2048815/93‑1 (MQ=255)
                                                   cGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGacac                                     <  1:2807242/93‑1 (MQ=255)
                                                    gATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACt                                    <  2:1978499/93‑1 (MQ=255)
                                                      tCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAc                                  <  1:4108075/93‑1 (MQ=255)
                                                      tCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAc                                  <  2:3752104/93‑1 (MQ=255)
                                                       ccGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTAcc                                 <  1:5356722/93‑1 (MQ=255)
                                                        cGTATCAACTCCCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGAAACTACCg                                >  2:3077557/1‑93 (MQ=255)
                                                        cGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCg                                <  2:1062857/93‑1 (MQ=255)
                                                         gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGt                               >  1:2993886/1‑93 (MQ=255)
                                                         gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGt                               >  2:586865/1‑93 (MQ=255)
                                                         gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGt                               <  2:5576170/93‑1 (MQ=255)
                                                         gTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGt                               <  1:988936/93‑1 (MQ=255)
                                                          tATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTg                              >  1:1260682/1‑93 (MQ=255)
                                                          tATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTg                              >  2:348062/1‑93 (MQ=255)
                                                          tATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTg                              >  2:5136692/1‑93 (MQ=255)
                                                           aTCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGc                             >  1:2151470/1‑93 (MQ=255)
                                                            tCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCa                            >  1:82641/1‑93 (MQ=255)
                                                            tCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCa                            <  1:442823/93‑1 (MQ=255)
                                                               aCTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAt                         >  1:3630124/1‑93 (MQ=255)
                                                               aCTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAt                         <  2:2518784/93‑1 (MQ=255)
                                                                cTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATg                        >  1:1965722/1‑93 (MQ=255)
                                                                  cACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAg                      <  2:4931395/93‑1 (MQ=255)
                                                                   aCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGc                     <  1:3078167/93‑1 (MQ=255)
                                                                   aCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGc                     <  1:916110/93‑1 (MQ=255)
                                                                     cGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGa                   >  2:4220214/1‑93 (MQ=255)
                                                                     cGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGa                   >  1:1990469/1‑93 (MQ=255)
                                                                     cGAAGAACAGTACCAGACGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGa                   <  1:4278035/93‑1 (MQ=255)
                                                                      gaagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAt                  >  1:2837241/1‑93 (MQ=255)
                                                                      gaagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAt                  >  2:5683804/1‑93 (MQ=255)
                                                                       aagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAtt                 <  2:2200679/93‑1 (MQ=255)
                                                                       aagaaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAtt                 <  1:390993/93‑1 (MQ=255)
                                                                          aaCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCa              <  1:3447072/93‑1 (MQ=255)
                                                                           aCAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaa             >  1:4849232/1‑93 (MQ=255)
                                                                            cAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaa            <  2:3306070/93‑1 (MQ=255)
                                                                            cAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaa            <  2:1114103/93‑1 (MQ=255)
                                                                              gTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaaaa          <  1:5708338/93‑1 (MQ=255)
                                                                              gTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCaaaaa          <  1:2401532/93‑1 (MQ=255)
                                                                                 ccAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGa       <  1:6013402/93‑1 (MQ=255)
                                                                                 ccAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGa       <  2:5353499/93‑1 (MQ=255)
                                                                                  cAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAg      <  2:4088125/93‑1 (MQ=255)
                                                                                  cAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAg      >  1:4127248/1‑93 (MQ=255)
                                                                                   aGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAgg     >  2:2636099/1‑93 (MQ=255)
                                                                                   aGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAgg     <  1:6260060/93‑1 (MQ=255)
                                                                                    ggcggcGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAggg    <  1:6178134/93‑1 (MQ=255)
                                                                                     gcggcgCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAgggg   >  1:935613/1‑92 (MQ=255)
                                                                                         |                                                                                         
TGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTG  >  NC_000913/1510250‑1510428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  NC_000913/1510246‑1510432
                                                                                             |                                                                                             
ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGC                                                                                               <  SRR4302533.1080339/94‑1 (MQ=60)
 TATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCG                                                                                              <  SRR4302533.5913613/94‑1 (MQ=60)
    TGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTG                                                                                           >  SRR4302533.355211/1‑94 (MQ=60)
     GCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGA                                                                                          <  SRR4302533.1068169/94‑1 (MQ=60)
       GGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAA                                                                                        >  SRR4302533.625702/1‑94 (MQ=60)
        GACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAG                                                                                       <  SRR4302533.2451547/94‑1 (MQ=60)
          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     >  SRR4302533.1039390/1‑94 (MQ=60)
          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     <  SRR4302533.4205473/94‑1 (MQ=60)
          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     <  SRR4302533.5576739/94‑1 (MQ=60)
          CGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTG                                                                                     >  SRR4302533.6242831/1‑94 (MQ=60)
           GCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGC                                                                                    >  SRR4302533.3543498/1‑94 (MQ=60)
           GCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGC                                                                                    >  SRR4302533.46853/1‑94 (MQ=60)
           GCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGC                                                                                    >  SRR4302533.2218244/1‑94 (MQ=60)
             TGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTG                                                                                  >  SRR4302533.937917/1‑94 (MQ=60)
               CGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCG                                                                                >  SRR4302533.3467963/1‑94 (MQ=60)
                GTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGC                                                                               <  SRR4302533.5049923/94‑1 (MQ=60)
                  TGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGC                                                                             >  SRR4302533.723978/1‑94 (MQ=60)
                  TGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGC                                                                             >  SRR4302533.4930339/1‑94 (MQ=60)
                  TGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGC                                                                             >  SRR4302533.2511972/1‑94 (MQ=60)
                   GTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCT                                                                            >  SRR4302533.2176830/1‑94 (MQ=60)
                   GTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCT                                                                            >  SRR4302533.4524467/1‑94 (MQ=60)
                   GTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCT                                                                            >  SRR4302533.1328367/1‑94 (MQ=60)
                    TTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTC                                                                           <  SRR4302533.6313150/94‑1 (MQ=60)
                     TCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCA                                                                          <  SRR4302533.202112/94‑1 (MQ=60)
                        TTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACA                                                                       >  SRR4302533.3400045/1‑94 (MQ=60)
                         TAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACAC                                                                      <  SRR4302533.1781155/94‑1 (MQ=60)
                          AAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACA                                                                     <  SRR4302533.3853347/94‑1 (MQ=60)
                           AAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAG                                                                    >  SRR4302533.1945495/1‑94 (MQ=60)
                             GCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTT                                                                  <  SRR4302533.1237412/94‑1 (MQ=60)
                             GCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTT                                                                  >  SRR4302533.1479432/1‑94 (MQ=60)
                             GCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTT                                                                  <  SRR4302533.3311714/94‑1 (MQ=60)
                               CACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTG                                                                <  SRR4302533.2016763/94‑1 (MQ=60)
                               CACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTG                                                                <  SRR4302533.606763/94‑1 (MQ=60)
                                 CTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGAT                                                              >  SRR4302533.499873/1‑94 (MQ=60)
                                   CAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCC                                                            <  SRR4302533.4305364/94‑1 (MQ=60)
                                     GCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAT                                                          <  SRR4302533.4624954/94‑1 (MQ=60)
                                        GCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGC                                                       <  SRR4302533.1997682/94‑1 (MQ=60)
                                         CAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCT                                                      >  SRR4302533.1490404/1‑94 (MQ=60)
                                           GTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTAC                                                    >  SRR4302533.387295/1‑94 (MQ=60)
                                                GCACCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCA                                               <  SRR4302533.2981744/94‑1 (MQ=60)
                                                 CATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCAT                                              >  SRR4302533.719380/1‑94 (MQ=60)
                                                     AGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACA                                          >  SRR4302533.3425001/1‑94 (MQ=60)
                                                     AGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACA                                          >  SRR4302533.2308573/1‑94 (MQ=60)
                                                      GCGATCCGTATCAACTCGCCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACTCAGTTTGATCCATCGCTACTGGCATGACAC                                         <  SRR4302533.888198/94‑1 (MQ=60)
                                                       CGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACT                                        <  SRR4302533.2824517/94‑1 (MQ=60)
                                                        GATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGGGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTA                                       >  SRR4302533.6307274/1‑94 (MQ=60)
                                                          TCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACC                                     <  SRR4302533.4080175/94‑1 (MQ=60)
                                                          TCCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACC                                     <  SRR4302533.4133051/94‑1 (MQ=60)
                                                           CCGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCG                                    <  SRR4302533.5388876/94‑1 (MQ=60)
                                                            CGTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGT                                   >  SRR4302533.3012190/1‑94 (MQ=60)
                                                             GTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTG                                  <  SRR4302533.994721/94‑1 (MQ=60)
                                                             GTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTG                                  >  SRR4302533.2717068/1‑94 (MQ=60)
                                                             GTATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTG                                  >  SRR4302533.1268222/1‑94 (MQ=60)
                                                              TATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGC                                 >  SRR4302533.2164720/1‑94 (MQ=60)
                                                              TATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGC                                 >  SRR4302533.825881/1‑94 (MQ=60)
                                                              TATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGC                                 <  SRR4302533.4179412/94‑1 (MQ=60)
                                                               ATCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCA                                >  SRR4302533.83165/1‑94 (MQ=60)
                                                                TCAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAA                               <  SRR4302533.445470/94‑1 (MQ=60)
                                                                 CAACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAA                              >  SRR4302533.2505436/1‑94 (MQ=60)
                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             >  SRR4302533.3652459/1‑94 (MQ=60)
                                                                  AACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAAT                             <  SRR4302533.6225921/94‑1 (MQ=60)
                                                                   ACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATG                            >  SRR4302533.1977732/1‑94 (MQ=60)
                                                                   ACTCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATG                            <  SRR4302533.3615290/94‑1 (MQ=60)
                                                                     TCACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAG                          <  SRR4302533.6318476/94‑1 (MQ=60)
                                                                      CACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGG                         >  SRR4302533.5363008/1‑94 (MQ=60)
                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        <  SRR4302533.3097056/94‑1 (MQ=60)
                                                                       ACCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCG                        <  SRR4302533.921493/94‑1 (MQ=60)
                                                                        CCGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGA                       >  SRR4302533.2002661/1‑94 (MQ=60)
                                                                         CGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAT                      >  SRR4302533.2854705/1‑94 (MQ=60)
                                                                         CGAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAT                      >  SRR4302533.6160379/1‑94 (MQ=60)
                                                                         CGAAGAACAGTACCAGACGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGAT                      <  SRR4302533.4303760/94‑1 (MQ=60)
                                                                          GAAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATT                     <  SRR4302533.4733506/94‑1 (MQ=60)
                                                                           AAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTT                    <  SRR4302533.1556349/94‑1 (MQ=60)
                                                                           AAGAACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTT                    <  SRR4302533.393364/94‑1 (MQ=60)
                                                                              AACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAA                 >  SRR4302533.4878618/1‑94 (MQ=60)
                                                                              AACAGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAA                 <  SRR4302533.3468358/94‑1 (MQ=60)
                                                                                 AGTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAA              >  SRR4302533.5974972/1‑94 (MQ=60)
                                                                                  GTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAAC             <  SRR4302533.5742818/94‑1 (MQ=60)
                                                                                  GTACCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAAC             <  SRR4302533.2416365/94‑1 (MQ=60)
                                                                                     CCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAG          <  SRR4302533.6049837/94‑1 (MQ=60)
                                                                                     CCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAG          <  SRR4302533.3060939/94‑1 (MQ=60)
                                                                                     CCAGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAG          >  SRR4302533.4152303/1‑94 (MQ=60)
                                                                                       AGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGG        <  SRR4302533.6297487/94‑1 (MQ=60)
                                                                                       AGGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGG        <  SRR4302533.6278825/94‑1 (MQ=60)
                                                                                        GGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGT       <  SRR4302533.6215245/94‑1 (MQ=60)
                                                                                        GGCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGG       >  SRR4302533.941105/1‑94 (MQ=60)
                                                                                         GCGGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTG      >  SRR4302533.4294771/1‑94 (MQ=60)
                                                                                           GGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTG    <  SRR4302533.2111679/94‑1 (MQ=60)
                                                                                           GGCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTG    <  SRR4302533.939173/94‑1 (MQ=60)
                                                                                            GCGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGG   <  SRR4302533.619611/94‑1 (MQ=60)
                                                                                             CGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  SRR4302533.1472928/1‑94 (MQ=60)
                                                                                             CGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  <  SRR4302533.380317/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGT  >  NC_000913/1510246‑1510432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑