Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,533,355	G→A	A176A (GCC→GCT)	yddE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,533,355	0	G	A	99.6%	929.5 / ‑5.4	255	A176A (GCC→GCT)	yddE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (113/141); total (113/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

Only 100 of 262 total aligned reads displayed.

GTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC  >  NC_000913/1533265‑1533444

gTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGAGAAAGAGc                                                                                         >  1:4944633/1‑93 (MQ=255)

 tttttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCg                                                                                        >  2:5031286/1‑93 (MQ=255)

  ttttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGt                                                                                       <  2:9893878/93‑1 (MQ=255)

    ttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCg                                                                                     <  1:450243/93‑1 (MQ=255)

      gCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGat                                                                                   <  1:4178727/93‑1 (MQ=255)

        cTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGatat                                                                                 <  1:8402287/93‑1 (MQ=255)

        cTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGatat                                                                                 >  1:2238131/1‑93 (MQ=255)

        cTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGatat                                                                                 <  1:1670971/93‑1 (MQ=255)

         tGGGCGGATCGGGAACGGGAAGAAACCATTCCAGCAAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATc                                                                                <  1:7037405/93‑1 (MQ=255)

          gggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATcc                                                                               >  2:923109/1‑93 (MQ=255)

          gggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGAGTCGATATcc                                                                               >  1:2533018/1‑93 (MQ=255)

           ggCGGATCTGGAACGGGAAGAACCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCa                                                                              <  1:266773/93‑1 (MQ=255)

           ggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCa                                                                              <  2:2610267/93‑1 (MQ=255)

            gCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCAc                                                                             <  1:1513085/93‑1 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          <  1:5133862/93‑1 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          >  1:7774225/1‑93 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          <  1:348890/93‑1 (MQ=255)

               gATATGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          <  1:9815383/93‑1 (MQ=255)

                aTCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTcc                                                                         <  2:7185665/93‑1 (MQ=255)

                 tCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCg                                                                        >  2:8081692/1‑93 (MQ=255)

                 tCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCg                                                                        >  2:6090175/1‑93 (MQ=255)

                   tGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGt                                                                      >  2:3227994/1‑93 (MQ=255)

                   tGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGt                                                                      >  2:2564877/1‑93 (MQ=255)

                     gAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGttt                                                                    <  1:7851206/93‑1 (MQ=255)

                     gAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGttt                                                                    <  2:6971287/93‑1 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   >  1:5519354/1‑93 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   <  2:7915581/93‑1 (MQ=255)

                       aCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCa                                                                  >  2:1848535/1‑93 (MQ=255)

                       aCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCa                                                                  <  1:9146345/93‑1 (MQ=255)

                       aCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCa                                                                  <  1:5926533/93‑1 (MQ=255)

                         gggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGt                                                                >  2:3965981/1‑93 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               >  1:6584136/1‑93 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               <  2:459337/93‑1 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               <  1:11328/93‑1 (MQ=255)

                           gaagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTgg                                                              <  2:6240611/93‑1 (MQ=255)

                              gaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGat                                                           <  1:1275977/93‑1 (MQ=255)

                              gaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGat                                                           >  2:4528150/1‑93 (MQ=255)

                              gaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGat                                                           >  2:8390530/1‑93 (MQ=255)

                              gaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGat                                                           <  1:3689365/93‑1 (MQ=255)

                               aacccATGGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                                          <  2:1699411/90‑1 (MQ=255)

                                acccATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCTCATTAAGGTCAGGCGAAAGAGCGTCGATATCCGCTTCCGGTTTCAGTGGGatca                                                         <  2:677829/91‑1 (MQ=255)

                                aaCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatca                                                         <  1:5810323/93‑1 (MQ=255)

                                 aCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatcat                                                        <  2:5844010/93‑1 (MQ=255)

                                  ccATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                                       >  1:9363141/1‑93 (MQ=255)

                                   cATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGAtcatca                                                      <  1:472497/93‑1 (MQ=255)

                                   cATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGAtcatca                                                      >  2:5935692/1‑93 (MQ=255)

                                    aTTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAc                                                     >  1:2528538/1‑93 (MQ=255)

                                    aTTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAc                                                     >  1:3451807/1‑93 (MQ=255)

                                      tGCAGCCAATCGTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGTGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtt                                                   <  2:2092696/93‑1 (MQ=255)

                                       gCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACttt                                                  <  1:8513383/93‑1 (MQ=255)

                                        cAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                                                 <  1:3899566/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                <  2:6287862/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                >  1:6194936/1‑93 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                <  2:6637280/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                >  2:6858205/1‑93 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                >  1:8144080/1‑93 (MQ=255)

                                          gCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGa                                               >  1:3021064/1‑93 (MQ=255)

                                          gCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGa                                               <  1:6098764/93‑1 (MQ=255)

                                           ccAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAg                                              >  1:9534737/1‑93 (MQ=255)

                                            cAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                                             >  2:364861/1‑93 (MQ=255)

                                             aaTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtg                                            <  2:1413905/93‑1 (MQ=255)

                                             aaTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtg                                            <  2:9740508/93‑1 (MQ=255)

                                              aTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtgt                                           >  2:1988877/1‑93 (MQ=255)

                                               tCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                                          <  2:7486351/93‑1 (MQ=255)

                                                cTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTcc                                         <  2:9603420/93‑1 (MQ=255)

                                                cTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTcc                                         <  2:7044053/93‑1 (MQ=255)

                                                 tttttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt                                        <  2:1181150/93‑1 (MQ=255)

                                                   tttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGTGATCATCACTTTTGAGTGTCCtgt                                      <  1:1971475/93‑1 (MQ=255)

                                                    ttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt                                     <  2:3732692/93‑1 (MQ=255)

                                                    ttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCCAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt                                     <  1:687688/93‑1 (MQ=255)

                                                      gCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt                                   <  1:8859891/93‑1 (MQ=255)

                                                            agaggTCAGCGCATTAAGGTCAGGCGAAGGAGCGTCGATATCCCCTCCCGGTGTCAGTGGGATCATCCCTTTTGAGTGTCCTGTTGTTGCCaa                             <  2:8241272/90‑2 (MQ=255)

                                                                ggcAGCGCAATAAAGGCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa                         <  1:3137367/91‑1 (MQ=255)

                                                                gTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa                         >  1:9597086/1‑93 (MQ=255)

                                                                gTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa                         <  2:87501/93‑1 (MQ=255)

                                                                 tCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa                        <  1:2152214/93‑1 (MQ=255)

                                                                  cAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt                       <  2:5494495/93‑1 (MQ=255)

                                                                   aGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt                      <  2:1492669/93‑1 (MQ=255)

                                                                   aGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt                      <  2:6803881/93‑1 (MQ=255)

                                                                    gcgcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg                     >  1:7275326/1‑93 (MQ=255)

                                                                    gcgcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg                     <  2:5597030/93‑1 (MQ=255)

                                                                        aatAAGGGCAGGAGAAAGAGCGTCCATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCaa                 <  1:7185293/91‑1 (MQ=255)

                                                                         ttAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAg                <  2:1319729/93‑1 (MQ=255)

                                                                         ttAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAg                <  2:2298943/93‑1 (MQ=255)

                                                                           aaGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc              >  2:646191/1‑93 (MQ=255)

                                                                           aaGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc              <  2:1100662/93‑1 (MQ=255)

                                                                           aaGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc              <  2:3108229/93‑1 (MQ=255)

                                                                             ggTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTg            <  2:506151/93‑1 (MQ=255)

                                                                             ggTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTg            <  2:7667855/93‑1 (MQ=255)

                                                                             ggTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTg            >  2:47658/1‑93 (MQ=255)

                                                                               tCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGc          >  2:9151759/1‑93 (MQ=255)

                                                                                 aGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAg        <  2:9695856/93‑1 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    <  1:8461689/93‑1 (MQ=255)

                                                                                      aaaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAatat   >  1:2748760/1‑93 (MQ=255)

                                                                                      aaaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAatat   >  2:5350094/1‑93 (MQ=255)

                                                                                      aaaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAatat   <  1:8168298/93‑1 (MQ=255)

                                                                                      aaaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAatat   >  1:6409040/1‑93 (MQ=255)

                                                                                       aaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATc  >  2:6386775/1‑93 (MQ=255)

                                                                                       aaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATc  >  1:5236290/1‑93 (MQ=255)

                                                                                       aaGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATc  <  2:3565166/93‑1 (MQ=255)

GTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC  >  NC_000913/1533265‑1533444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTC  >  NC_000913/1533262‑1533447

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGA                                                                                              <  SRR4302540.648854/94‑1 (MQ=60)

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGA                                                                                              >  SRR4302540.2959307/1‑94 (MQ=60)

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGA                                                                                              <  SRR4302540.2950482/94‑1 (MQ=60)

 TCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAG                                                                                             <  SRR4302540.5930155/94‑1 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGC                                                                                            <  SRR4302540.4145635/94‑1 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGC                                                                                            >  SRR4302540.8556430/1‑94 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGC                                                                                            >  SRR4302540.4998010/1‑94 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGC                                                                                            <  SRR4302540.1647683/94‑1 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGAGAAAGAGC                                                                                            >  SRR4302540.4998009/1‑94 (MQ=60)

    TTTTTGCCTGGGCGGATCTGGAACGGTAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGT                                                                                          <  SRR4302540.6429804/94‑1 (MQ=60)

    TTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGT                                                                                          <  SRR4302540.963832/94‑1 (MQ=60)

     TTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTC                                                                                         <  SRR4302540.899521/94‑1 (MQ=60)

       TTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGA                                                                                       <  SRR4302540.455038/94‑1 (MQ=60)

       TTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGA                                                                                       <  SRR4302540.455037/94‑1 (MQ=60)

        TGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGAT                                                                                      >  SRR4302540.7313764/1‑94 (MQ=60)

         GCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATA                                                                                     <  SRR4302540.4224098/94‑1 (MQ=60)

         GCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATA                                                                                     >  SRR4302540.6110018/1‑94 (MQ=60)

          CCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATAT                                                                                    >  SRR4302540.2262199/1‑94 (MQ=60)

           CTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATC                                                                                   <  SRR4302540.8499803/94‑1 (MQ=60)

           CTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATC                                                                                   <  SRR4302540.1689143/94‑1 (MQ=60)

           CTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATC                                                                                   <  SRR4302540.399290/94‑1 (MQ=60)

           CTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATC                                                                                   >  SRR4302540.1200897/1‑94 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                  >  SRR4302540.553288/1‑94 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                  <  SRR4302540.2360007/94‑1 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                  <  SRR4302540.7115886/94‑1 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                  <  SRR4302540.5215102/94‑1 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGAGTCGATATCC                                                                                  >  SRR4302540.2560344/1‑94 (MQ=60)

            TGGGCGGATCGGGAACGGGAAGAAACCATTCCAGCAAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                  <  SRR4302540.7115636/94‑1 (MQ=60)

             GGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCA                                                                                 <  SRR4302540.4226799/94‑1 (MQ=60)

              GGCGGATCTGGAACGGGAAGAACCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCAC                                                                                <  SRR4302540.269400/94‑1 (MQ=60)

               GCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACT                                                                               <  SRR4302540.8974704/94‑1 (MQ=60)

               GCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACT                                                                               <  SRR4302540.1529807/94‑1 (MQ=60)

                CGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTT                                                                              >  SRR4302540.7768851/1‑94 (MQ=60)

                 GGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTC                                                                             >  SRR4302540.1741816/1‑94 (MQ=60)

                 GGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTC                                                                             >  SRR4302540.7865108/1‑94 (MQ=60)

                  GATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                            <  SRR4302540.5189463/94‑1 (MQ=60)

                  GATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                            <  SRR4302540.352448/94‑1 (MQ=60)

                  GATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                            <  SRR4302540.6898436/94‑1 (MQ=60)

                  GATATGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                            <  SRR4302540.9930239/94‑1 (MQ=60)

                   ATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCG                                                                           >  SRR4302540.1622801/1‑94 (MQ=60)

                   ATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCG                                                                           <  SRR4302540.2718409/94‑1 (MQ=60)

                      TGGAACGGGAAGAAGCCATGGCCGCGAATCTTGTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGATCGTCGATATCCACTTCCGGTT                                                                        <  SRR4302540.5303167/94‑1 (MQ=60)

                       GGAACGGGAAGAAACCATTGCAGCCAATCTTGTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTT                                                                       <  SRR4302540.7384040/94‑1 (MQ=60)

                       GGAACGGGAAGAAACCATTGCAGCCAATCGTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTT                                                                       <  SRR4302540.8393667/94‑1 (MQ=60)

                        GAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTC                                                                      <  SRR4302540.7942947/94‑1 (MQ=60)

                        GAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTC                                                                      >  SRR4302540.2794538/1‑94 (MQ=60)

                        GAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTC                                                                      >  SRR4302540.5580143/1‑94 (MQ=60)

                          ACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCTGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAG                                                                    <  SRR4302540.5992017/94‑1 (MQ=60)

                          ACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAG                                                                    <  SRR4302540.9256200/94‑1 (MQ=60)

                          ACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAG                                                                    <  SRR4302540.5992016/94‑1 (MQ=60)

                           CGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGT                                                                   <  SRR4302540.2776777/94‑1 (MQ=60)

                           CGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGT                                                                   <  SRR4302540.8167153/94‑1 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                  >  SRR4302540.6657066/1‑94 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                  <  SRR4302540.7896916/94‑1 (MQ=60)

                             GGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGG                                                                 <  SRR4302540.11449/94‑1 (MQ=60)

                              GAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGG                                                                >  SRR4302540.3165978/1‑94 (MQ=60)

                               AAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGA                                                               >  SRR4302540.2592634/1‑94 (MQ=60)

                                AGAAACCATTGGAGCCAATCTGTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGAT                                                              <  SRR4302540.5025314/94‑1 (MQ=60)

                                AGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGAT                                                              >  SRR4302540.6870971/1‑94 (MQ=60)

                                 GAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATC                                                             <  SRR4302540.3729668/94‑1 (MQ=60)

                                 GAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATC                                                             <  SRR4302540.6995130/94‑1 (MQ=60)

                                 GAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATC                                                             <  SRR4302540.1289130/94‑1 (MQ=60)

                                   AACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCAT                                                           <  SRR4302540.5874541/94‑1 (MQ=60)

                                   AACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATAAT                                                           <  SRR4302540.8583367/94‑1 (MQ=60)

                                    ACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATC                                                          >  SRR4302540.9474637/1‑94 (MQ=60)

                                    ACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATC                                                          <  SRR4302540.595095/94‑1 (MQ=60)

                                     CCATTTCAGCCAATCTTTTTGATGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCA                                                         <  SRR4302540.2686301/94‑1 (MQ=60)

                                     CCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCA                                                         >  SRR4302540.7337476/1‑94 (MQ=60)

                                      CATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAC                                                        >  SRR4302540.3489354/1‑94 (MQ=60)

                                      CATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAC                                                        <  SRR4302540.477557/94‑1 (MQ=60)

                                      CATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAC                                                        >  SRR4302540.3361014/1‑94 (MQ=60)

                                      CATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAC                                                        >  SRR4302540.2555824/1‑94 (MQ=60)

                                      CATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAC                                                        >  SRR4302540.2555823/1‑94 (MQ=60)

                                       ATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACT                                                       <  SRR4302540.10063733/94‑1 (MQ=60)

                                          GCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTT                                                    <  SRR4302540.8612320/94‑1 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTGGATATCCACTTCCGGTTTCAGTGGGATCATCATTTTTG                                                   >  SRR4302540.8238612/1‑94 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                   >  SRR4302540.6263909/1‑94 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                   >  SRR4302540.6241847/1‑94 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                   >  SRR4302540.8238611/1‑94 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                   <  SRR4302540.3941880/94‑1 (MQ=60)

                                            AGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGA                                                  >  SRR4302540.3054267/1‑94 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                 <  SRR4302540.9573739/94‑1 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                 >  SRR4302540.9647608/1‑94 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                 <  SRR4302540.209647/94‑1 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                 <  SRR4302540.6166662/94‑1 (MQ=60)

                                              CCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGT                                                <  SRR4302540.1692509/94‑1 (MQ=60)

                                               CAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTG                                               <  SRR4302540.4673150/94‑1 (MQ=60)

                                                     TTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGT                                         >  SRR4302540.9374034/1‑94 (MQ=60)

                                                      TTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGTGATCATCACTTTTGAGTGTCCTGTT                                        <  SRR4302540.1992879/94‑1 (MQ=60)

                                                       TTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCCAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTG                                       <  SRR4302540.695071/94‑1 (MQ=60)

                                                        TGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGT                                      >  SRR4302540.8960541/1‑94 (MQ=60)

                                                         GCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTT                                     <  SRR4302540.8962413/94‑1 (MQ=60)

                                                         GCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTT                                     >  SRR4302540.962152/1‑94 (MQ=60)

                                                          CTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTG                                    >  SRR4302540.3846438/1‑94 (MQ=60)

                                                          CTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTG                                    >  SRR4302540.8444390/1‑94 (MQ=60)

                                                              TAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAC                                <  SRR4302540.6500612/94‑1 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                            >  SRR4302540.9710324/1‑94 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                            <  SRR4302540.2542937/94‑1 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                            >  SRR4302540.2209265/1‑94 (MQ=60)

                                                                   GTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA                           <  SRR4302540.3679023/94‑1 (MQ=60)

                                                                   GGCAGCGCAATAAAGGCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA                           <  SRR4302540.3171583/94‑1 (MQ=60)

                                                                    TCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAT                          <  SRR4302540.2175362/94‑1 (MQ=60)

                                                                    TCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAT                          <  SRR4302540.7080811/94‑1 (MQ=60)

                                                                     CAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATT                         >  SRR4302540.3497766/1‑94 (MQ=60)

                                                                      AGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTG                        >  SRR4302540.7356504/1‑94 (MQ=60)

                                                                        CGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGC                      >  SRR4302540.5713283/1‑94 (MQ=60)

                                                                        CGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGC                      >  SRR4302540.4927667/1‑94 (MQ=60)

                                                                         GCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCA                     <  SRR4302540.1498662/94‑1 (MQ=60)

                                                                         GCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCA                     >  SRR4302540.5947702/1‑94 (MQ=60)

                                                                          CATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAA                    <  SRR4302540.9974225/94‑1 (MQ=60)

                                                                          CATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAA                    >  SRR4302540.3671129/1‑94 (MQ=60)

                                                                           ATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAG                   <  SRR4302540.8533486/94‑1 (MQ=60)

                                                                           AATAAGGGCAGGAGAAAGAGCGTCCATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAG                   <  SRR4302540.7265392/94‑1 (MQ=60)

                                                                              AAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCT                <  SRR4302540.8766626/94‑1 (MQ=60)

                                                                               AGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTG               >  SRR4302540.4800204/1‑94 (MQ=60)

                                                                               AGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTG               >  SRR4302540.3157653/1‑94 (MQ=60)

                                                                                GGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGG              <  SRR4302540.2014120/94‑1 (MQ=60)

                                                                                  TCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCA            >  SRR4302540.4221026/1‑94 (MQ=60)

                                                                                    AGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCATA          >  SRR4302540.7957952/1‑94 (MQ=60)

                                                                                    AGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGA          <  SRR4302540.1402022/94‑1 (MQ=60)

                                                                                       CGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATA       <  SRR4302540.6335655/94‑1 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT      <  SRR4302540.9894399/94‑1 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT      <  SRR4302540.8559942/94‑1 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT      <  SRR4302540.9894398/94‑1 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT      >  SRR4302540.6480371/1‑94 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT      >  SRR4302540.2778458/1‑94 (MQ=60)

                                                                                         AAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC     <  SRR4302540.8263157/94‑1 (MQ=60)

                                                                                         AAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC     >  SRR4302540.3175615/1‑94 (MQ=60)

                                                                                         AAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC     >  SRR4302540.5293410/1‑94 (MQ=60)

                                                                                          AAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCG    >  SRR4302540.7405264/1‑94 (MQ=60)

                                                                                           AGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGT   >  SRR4302540.9527857/1‑94 (MQ=60)

                                                                                           AGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGT   >  SRR4302540.4890541/1‑94 (MQ=60)

                                                                                           AGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGT   <  SRR4302540.6344300/94‑1 (MQ=60)

                                                                                           AGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGT   >  SRR4302540.8246756/1‑94 (MQ=60)

                                                                                            GAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTC  <  SRR4302540.9505745/94‑1 (MQ=60)

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTC  >  NC_000913/1533262‑1533447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`