Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,533,355	G→A	A176A (GCC→GCT)	yddE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,533,355	0	G	A	99.5%	798.9 / NA	212	A176A (GCC→GCT)	yddE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (102/109); minor base C (1/0); total (103/109)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC  >  NC_000913/1533265‑1533444

  ttttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGt                                                                                       <  1:4071438/93‑1 (MQ=255)

   tttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTc                                                                                      <  2:911276/93‑1 (MQ=255)

   tttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTc                                                                                      <  1:1570188/93‑1 (MQ=255)

   tttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTc                                                                                      <  1:4290603/93‑1 (MQ=255)

     tGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGa                                                                                    >  2:1289465/1‑93 (MQ=255)

      gCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGat                                                                                   <  2:330664/93‑1 (MQ=255)

       ccTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGata                                                                                  <  1:1026510/93‑1 (MQ=255)

          gggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATcc                                                                               <  2:2768948/93‑1 (MQ=255)

          gggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATcc                                                                               <  1:5542931/93‑1 (MQ=255)

          gggCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATcc                                                                               >  1:1424833/1‑93 (MQ=255)

            gCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCAc                                                                             <  2:2912750/93‑1 (MQ=255)

            gCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGACATCCAc                                                                             <  2:4256529/93‑1 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          >  2:4157629/1‑93 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          >  1:5109949/1‑93 (MQ=255)

               gATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTc                                                                          >  2:5875418/1‑93 (MQ=255)

                aTCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTcc                                                                         >  1:243565/1‑93 (MQ=255)

                 tCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCg                                                                        >  1:2630699/1‑93 (MQ=255)

                  cTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCgg                                                                       <  2:662150/93‑1 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   <  1:3857138/93‑1 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   <  1:355415/93‑1 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   >  2:5738322/1‑93 (MQ=255)

                      aaCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   <  2:6259273/93‑1 (MQ=255)

                      aaCGAGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTc                                                                   <  1:6258577/93‑1 (MQ=255)

                       aCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCa                                                                  <  1:5039927/93‑1 (MQ=255)

                       aCGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCa                                                                  >  2:3018395/1‑93 (MQ=255)

                         gggAATAAACCATGGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGt                                                                <  2:5252270/93‑1 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGTGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               >  1:764460/1‑93 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               >  1:3126414/1‑93 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               <  1:2260620/93‑1 (MQ=255)

                          ggAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTg                                                               <  1:1535651/93‑1 (MQ=255)

                           gaagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTgg                                                              >  1:5355692/1‑93 (MQ=255)

                           gaagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTgg                                                              <  2:5327198/93‑1 (MQ=255)

                           gaagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTgg                                                              <  2:1464163/93‑1 (MQ=255)

                            aagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTggg                                                             >  1:2642600/1‑93 (MQ=255)

                            aagaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTggg                                                             >  2:4328936/1‑93 (MQ=255)

                              gaaACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGat                                                           >  1:4459961/1‑93 (MQ=255)

                               aaaCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                                          >  2:4045678/1‑93 (MQ=255)

                               aaaCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                                          <  1:2535902/93‑1 (MQ=255)

                                aaCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatca                                                         >  2:1594291/1‑93 (MQ=255)

                                 aCCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatcat                                                        >  2:1748176/1‑93 (MQ=255)

                                  ccATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                                       <  2:1371128/93‑1 (MQ=255)

                                     ttGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt                                                    >  2:262569/1‑93 (MQ=255)

                                      tGCGGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtt                                                   <  1:3649411/93‑1 (MQ=255)

                                      tGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtt                                                   <  1:5726147/93‑1 (MQ=255)

                                        cAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                                                 <  1:433588/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                <  2:1865572/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                <  2:4412493/93‑1 (MQ=255)

                                         aGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                                                >  2:1485309/1‑93 (MQ=255)

                                            cAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                                             <  2:5624122/93‑1 (MQ=255)

                                             aaTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtg                                            <  1:2694476/93‑1 (MQ=255)

                                             aaTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtg                                            >  1:322711/1‑93 (MQ=255)

                                              aTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtgt                                           >  1:5869027/1‑93 (MQ=255)

                                              aTCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtgt                                           >  2:53500/1‑93 (MQ=255)

                                               tCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                                          <  1:1238421/93‑1 (MQ=255)

                                               tCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                                          >  1:4193177/1‑93 (MQ=255)

                                               tCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                                          >  1:5224255/1‑93 (MQ=255)

                                               tCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                                          <  1:1283925/93‑1 (MQ=255)

                                                cTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTcc                                         >  2:606049/1‑93 (MQ=255)

                                                 tttttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt                                        >  2:2099966/1‑93 (MQ=255)

                                                 tttttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt                                        <  2:5572202/93‑1 (MQ=255)

                                                   tatGCTGATAGCGGTAAGCGCATTAAGGTCAGGAGAAAGAGCGTCGATATCCACTACCGGTTTCAGTGGGATCATAACTTTTGAGTGTCAtgt                                      <  1:6297801/91‑1 (MQ=255)

                                                    ttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt                                     <  1:2126836/93‑1 (MQ=255)

                                                      gCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt                                   >  1:4745870/1‑93 (MQ=255)

                                                       cTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt                                  <  1:4200106/93‑1 (MQ=255)

                                                       cTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt                                  <  1:2363857/93‑1 (MQ=255)

                                                         gATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc                                >  1:2023584/1‑93 (MQ=255)

                                                           tAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCa                              >  1:18574/1‑93 (MQ=255)

                                                            aGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc                             >  1:2741990/1‑93 (MQ=255)

                                                             gCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc                            <  2:3999896/93‑1 (MQ=255)

                                                             gCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc                            >  2:2460201/1‑93 (MQ=255)

                                                                gTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa                         >  1:5707027/1‑93 (MQ=255)

                                                                 tCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa                        >  1:5887377/1‑93 (MQ=255)

                                                                 tCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa                        <  1:3029854/93‑1 (MQ=255)

                                                                  cAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt                       >  1:6059286/1‑93 (MQ=255)

                                                                   aGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt                      >  1:5582678/1‑93 (MQ=255)

                                                                    gcgcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg                     >  2:5294237/1‑93 (MQ=255)

                                                                    gcgcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg                     >  1:2995349/1‑93 (MQ=255)

                                                                    gcgcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTACTGTTGTTGCCACCTGAATTg                     >  2:4731905/1‑93 (MQ=255)

                                                                      gcATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGc                   <  2:2217813/93‑1 (MQ=255)

                                                                        aTTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCaa                 >  1:2226593/1‑93 (MQ=255)

                                                                        aTTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCaa                 <  2:5080563/93‑1 (MQ=255)

                                                                        aTTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCaa                 >  1:6244855/1‑93 (MQ=255)

                                                                         ttAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAg                >  2:2879411/1‑93 (MQ=255)

                                                                           aaGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc              <  1:3356319/93‑1 (MQ=255)

                                                                            aGGTCAGGCGAAATAGCTTCGATATCCACGTCCGGTTTCAGTGGGATCATCGCTTTTCAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCt             <  2:1979011/93‑1 (MQ=255)

                                                                            aGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCt             <  2:1986936/93‑1 (MQ=255)

                                                                            aGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCt             <  1:3455210/93‑1 (MQ=255)

                                                                             ggTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTg            <  2:3729830/93‑1 (MQ=255)

                                                                              gTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTgg           <  1:4559350/93‑1 (MQ=255)

                                                                              gTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTgg           >  2:3181118/1‑93 (MQ=255)

                                                                                cAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCa         <  1:3693137/93‑1 (MQ=255)

                                                                                cAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCa         <  1:4430415/93‑1 (MQ=255)

                                                                                 aGGCGAAAGCGCGTCGATATCCACTTCCGGTTTCAGAGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAg        >  2:6029892/1‑93 (MQ=255)

                                                                                  ggCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGa       >  1:2867741/1‑93 (MQ=255)

                                                                                   gCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGaa      >  1:884821/1‑93 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    >  1:1118864/1‑93 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    >  1:1836778/1‑93 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    <  1:3316678/93‑1 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    >  2:3682952/1‑93 (MQ=255)

                                                                                     gAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAata    >  2:2315140/1‑93 (MQ=255)

GTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATC  >  NC_000913/1533265‑1533444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCC  >  NC_000913/1533262‑1533448

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGA                                                                                               >  SRR4302533.390837/1‑94 (MQ=60)

 TCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAG                                                                                              <  SRR4302533.206555/94‑1 (MQ=60)

 TCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAG                                                                                              <  SRR4302533.6147200/94‑1 (MQ=60)

 TCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAG                                                                                              <  SRR4302533.4853348/94‑1 (MQ=60)

  CGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGC                                                                                             >  SRR4302533.2231503/1‑94 (MQ=60)

   GTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCG                                                                                            <  SRR4302533.5607223/94‑1 (MQ=60)

    TTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGT                                                                                           <  SRR4302533.4478829/94‑1 (MQ=60)

     TTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTC                                                                                          <  SRR4302533.4096224/94‑1 (MQ=60)

      TTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCG                                                                                         <  SRR4302533.4316407/94‑1 (MQ=60)

      TTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCG                                                                                         <  SRR4302533.1579646/94‑1 (MQ=60)

      TTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCG                                                                                         >  SRR4302533.225617/1‑94 (MQ=60)

        TGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGAT                                                                                       <  SRR4302533.1690349/94‑1 (MQ=60)

          CCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATAT                                                                                     <  SRR4302533.1032541/94‑1 (MQ=60)

            TGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCC                                                                                   >  SRR4302533.1433451/1‑94 (MQ=60)

             GGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCA                                                                                  <  SRR4302533.5576406/94‑1 (MQ=60)

               GCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACT                                                                                <  SRR4302533.3197000/94‑1 (MQ=60)

               GCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACT                                                                                <  SRR4302533.81972/94‑1 (MQ=60)

                CGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTT                                                                               <  SRR4302533.3254921/94‑1 (MQ=60)

                CGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTT                                                                               >  SRR4302533.628246/1‑94 (MQ=60)

                 GGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTC                                                                              >  SRR4302533.5072681/1‑94 (MQ=60)

                 GGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTC                                                                              >  SRR4302533.5140830/1‑94 (MQ=60)

                  GATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                             >  SRR4302533.245075/1‑94 (MQ=60)

                  GATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCC                                                                             <  SRR4302533.3519018/94‑1 (MQ=60)

                   ATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCG                                                                            <  SRR4302533.36273/94‑1 (MQ=60)

                   ATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCG                                                                            >  SRR4302533.2646864/1‑94 (MQ=60)

                    TCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGG                                                                           <  SRR4302533.1852706/94‑1 (MQ=60)

                     CTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGT                                                                          >  SRR4302533.1564532/1‑94 (MQ=60)

                         AACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCA                                                                      <  SRR4302533.6176154/94‑1 (MQ=60)

                         AACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCA                                                                      <  SRR4302533.357581/94‑1 (MQ=60)

                         AACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCA                                                                      >  SRR4302533.4028706/1‑94 (MQ=60)

                         AACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCA                                                                      <  SRR4302533.3880687/94‑1 (MQ=60)

                         AACGAGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCA                                                                      <  SRR4302533.6296001/94‑1 (MQ=60)

                          ACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAG                                                                     <  SRR4302533.5070438/94‑1 (MQ=60)

                           CGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGT                                                                    <  SRR4302533.5635919/94‑1 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGTGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   >  SRR4302533.768963/1‑94 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   >  SRR4302533.2888048/1‑94 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   >  SRR4302533.3145633/1‑94 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   <  SRR4302533.3051094/94‑1 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   <  SRR4302533.6008100/94‑1 (MQ=60)

                            GGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATGAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTG                                                                   <  SRR4302533.4220964/94‑1 (MQ=60)

                             GGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGG                                                                  <  SRR4302533.1544877/94‑1 (MQ=60)

                             GGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGG                                                                  <  SRR4302533.2274604/94‑1 (MQ=60)

                             GGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGG                                                                  >  SRR4302533.5387841/1‑94 (MQ=60)

                             GGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGG                                                                  <  SRR4302533.3775223/94‑1 (MQ=60)

                              GAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGG                                                                 >  SRR4302533.2658838/1‑94 (MQ=60)

                              GAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGG                                                                 >  SRR4302533.3451180/1‑94 (MQ=60)

                               AAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGA                                                                <  SRR4302533.1318634/94‑1 (MQ=60)

                                AGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGAT                                                               >  SRR4302533.4486940/1‑94 (MQ=60)

                                 GAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATC                                                              <  SRR4302533.1528735/94‑1 (MQ=60)

                                  AAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCA                                                             <  SRR4302533.2551512/94‑1 (MQ=60)

                                   AACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCAT                                                            <  SRR4302533.3201810/94‑1 (MQ=60)

                                   AACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCAT                                                            <  SRR4302533.4509472/94‑1 (MQ=60)

                                         TGCGGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTT                                                      <  SRR4302533.3671850/94‑1 (MQ=60)

                                         TGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTT                                                      <  SRR4302533.3700431/94‑1 (MQ=60)

                                         TGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTT                                                      <  SRR4302533.5760718/94‑1 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                    <  SRR4302533.2792833/94‑1 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                    <  SRR4302533.436173/94‑1 (MQ=60)

                                           CAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTG                                                    >  SRR4302533.1264580/1‑94 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCTCTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                  <  SRR4302533.310668/94‑1 (MQ=60)

                                             GCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                  <  SRR4302533.5826972/94‑1 (MQ=60)

                                             GCCAATCTTTTTGCCGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAG                                                  <  SRR4302533.4203582/94‑1 (MQ=60)

                                              CCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGT                                                 >  SRR4302533.5626152/1‑94 (MQ=60)

                                               CAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTG                                                >  SRR4302533.324682/1‑94 (MQ=60)

                                               CAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTG                                                <  SRR4302533.2677554/94‑1 (MQ=60)

                                                AATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGT                                               >  SRR4302533.6309674/1‑94 (MQ=60)

                                                AATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGT                                               >  SRR4302533.5904499/1‑94 (MQ=60)

                                                AATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGT                                               >  SRR4302533.4770503/1‑94 (MQ=60)

                                                AATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGT                                               <  SRR4302533.2710988/94‑1 (MQ=60)

                                                 ATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTC                                              >  SRR4302533.5255683/1‑94 (MQ=60)

                                                 ATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTC                                              >  SRR4302533.4218556/1‑94 (MQ=60)

                                                  TCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCC                                             <  SRR4302533.1291649/94‑1 (MQ=60)

                                                  TCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCC                                             <  SRR4302533.1245821/94‑1 (MQ=60)

                                                  TCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCC                                             >  SRR4302533.3038140/1‑94 (MQ=60)

                                                  TCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCC                                             <  SRR4302533.4921770/94‑1 (MQ=60)

                                                   CTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCT                                            >  SRR4302533.653893/1‑94 (MQ=60)

                                                    TTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTG                                           <  SRR4302533.5755277/94‑1 (MQ=60)

                                                    TTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTG                                           >  SRR4302533.2323357/1‑94 (MQ=60)

                                                      TATGCTGATAGCGGTAAGCGCATTAAGGTCAGGAGAAAGAGCGTCGATATCCACTACCGGTTTCAGTGGGATCATAACTTTTGAGTGTCATGTT                                         <  SRR4302533.6335389/94‑1 (MQ=60)

                                                       TTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTG                                        <  SRR4302533.2139951/94‑1 (MQ=60)

                                                        TGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGT                                       >  SRR4302533.4774643/1‑94 (MQ=60)

                                                          CTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTG                                     <  SRR4302533.2378478/94‑1 (MQ=60)

                                                          CTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTG                                     <  SRR4302533.4225521/94‑1 (MQ=60)

                                                           TGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGC                                    >  SRR4302533.5250480/1‑94 (MQ=60)

                                                           TGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGC                                    >  SRR4302533.2036079/1‑94 (MQ=60)

                                                             ATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCA                                  >  SRR4302533.18699/1‑94 (MQ=60)

                                                              TAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAC                                 >  SRR4302533.244217/1‑94 (MQ=60)

                                                              TAGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAC                                 >  SRR4302533.2758803/1‑94 (MQ=60)

                                                               AGCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACC                                <  SRR4302533.3339014/94‑1 (MQ=60)

                                                                GCGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCT                               <  SRR4302533.4436427/94‑1 (MQ=60)

                                                                 CGGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTG                              <  SRR4302533.6328870/94‑1 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                             >  SRR4302533.5741498/1‑94 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                             >  SRR4302533.5433169/1‑94 (MQ=60)

                                                                  GGTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA                             >  SRR4302533.186891/1‑94 (MQ=60)

                                                                   GTCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA                            >  SRR4302533.5922948/1‑94 (MQ=60)

                                                                    TCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAT                           >  SRR4302533.6095968/1‑94 (MQ=60)

                                                                    TCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAT                           <  SRR4302533.2031443/94‑1 (MQ=60)

                                                                    TCAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAT                           <  SRR4302533.3048395/94‑1 (MQ=60)

                                                                     CAGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATT                          >  SRR4302533.5616408/1‑94 (MQ=60)

                                                                      AGCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTG                         >  SRR4302533.3013668/1‑94 (MQ=60)

                                                                       GCGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGG                        <  SRR4302533.2648581/94‑1 (MQ=60)

                                                                        CGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGC                       >  SRR4302533.2045034/1‑94 (MQ=60)

                                                                        CGCATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGG‑TTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCA                      >  SRR4302533.3817281/1‑94 (MQ=60)

                                                                          CATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAA                     >  SRR4302533.2240338/1‑94 (MQ=60)

                                                                          CATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAA                     >  SRR4302533.5374890/1‑94 (MQ=60)

                                                                          CATTAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAA                     >  SRR4302533.6282235/1‑94 (MQ=60)

                                                                             TAAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCC                  >  SRR4302533.422561/1‑94 (MQ=60)

                                                                              AAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCT                 <  SRR4302533.2608022/94‑1 (MQ=60)

                                                                              AAGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCT                 <  SRR4302533.3377056/94‑1 (MQ=60)

                                                                               AGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTG                <  SRR4302533.4355231/94‑1 (MQ=60)

                                                                               AGGTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTG                <  SRR4302533.3476536/94‑1 (MQ=60)

                                                                                 GTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGC              >  SRR4302533.1090743/1‑94 (MQ=60)

                                                                                 GTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGC              <  SRR4302533.1136158/94‑1 (MQ=60)

                                                                                 GTCAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGC              <  SRR4302533.4587003/94‑1 (MQ=60)

                                                                                   CAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAG            >  SRR4302533.5914115/1‑94 (MQ=60)

                                                                                   CAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAG            <  SRR4302533.4457197/94‑1 (MQ=60)

                                                                                   CAGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAG            <  SRR4302533.3715825/94‑1 (MQ=60)

                                                                                    AGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGA           >  SRR4302533.2885384/1‑94 (MQ=60)

                                                                                    AGGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGA           >  SRR4302533.2483029/1‑94 (MQ=60)

                                                                                     GGCGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAA          >  SRR4302533.890019/1‑94 (MQ=60)

                                                                                       CGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATA        >  SRR4302533.257665/1‑94 (MQ=60)

                                                                                       CGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATA        >  SRR4302533.1847980/1‑94 (MQ=60)

                                                                                       CGAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATA        >  SRR4302533.1125500/1‑94 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT       <  SRR4302533.3337161/94‑1 (MQ=60)

                                                                                        GAAAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATAT       >  SRR4302533.4562395/1‑94 (MQ=60)

                                                                                          AAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCG     >  SRR4302533.3258265/1‑94 (MQ=60)

                                                                                          AAGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCG     <  SRR4302533.2739669/94‑1 (MQ=60)

                                                                                           AGAGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGT    >  SRR4302533.6182958/1‑94 (MQ=60)

                                                                                             AGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCC  <  SRR4302533.2513154/94‑1 (MQ=60)

                                                                                             AGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCC  <  SRR4302533.3552947/94‑1 (MQ=60)

TTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCC  >  NC_000913/1533262‑1533448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`