Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,566,541 T→C I103V (ATA→GTA)  b1491 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,566,5410TC100.0% 714.6 / NA 219I103V (ATA→GTA) b1491hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (95/124);  total (95/124)

Only 100 of 226 total aligned reads displayed.

TCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGG  >  NC_000913/1566451‑1566630
                                                                                          |                                                                                         
tCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGc                                                                                         >  2:380942/1‑93 (MQ=255)
tCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGc                                                                                         >  2:4305529/1‑93 (MQ=255)
  ttACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCg                                                                                       <  1:807144/93‑1 (MQ=255)
  ttACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCg                                                                                       >  2:7077801/1‑93 (MQ=255)
      cGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGAc                                                                                   <  1:5366816/93‑1 (MQ=255)
        gTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGt                                                                                 <  1:5845609/93‑1 (MQ=255)
        gTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGt                                                                                 >  2:6345388/1‑93 (MQ=255)
        gTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGACGAGACGt                                                                                 >  1:2313415/1‑93 (MQ=255)
         tCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGtt                                                                                <  1:9971418/93‑1 (MQ=255)
            tAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCa                                                                             >  2:358092/1‑93 (MQ=255)
              aGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAga                                                                           >  2:2060287/1‑93 (MQ=255)
              aGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAga                                                                           <  1:3365710/93‑1 (MQ=255)
              aGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAga                                                                           <  1:1377900/93‑1 (MQ=255)
                      ccACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAg                                                                   >  2:7982118/1‑93 (MQ=255)
                       cACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGt                                                                  >  2:4934516/1‑93 (MQ=255)
                       cACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGt                                                                  <  1:6783421/93‑1 (MQ=255)
                       cACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGt                                                                  <  2:9765863/93‑1 (MQ=255)
                          ggCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTg                                                               >  1:3813686/1‑93 (MQ=255)
                           gCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGt                                                              >  1:9776665/1‑93 (MQ=255)
                             aaaaTTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCg                                                            >  2:9384989/1‑93 (MQ=255)
                               aaTTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGat                                                          >  2:461488/1‑93 (MQ=255)
                                aTTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatc                                                         <  2:5820657/93‑1 (MQ=255)
                                 ttttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatca                                                        <  1:4622636/93‑1 (MQ=255)
                                  tttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGTTCCAGTTTGTCGatcat                                                       >  1:7419843/1‑93 (MQ=255)
                                  tttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatcat                                                       <  1:7269866/93‑1 (MQ=255)
                                  tttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatcat                                                       <  2:7434873/93‑1 (MQ=255)
                                  tttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatcat                                                       <  1:8164685/93‑1 (MQ=255)
                                   ttCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGatcatc                                                      <  1:4380648/93‑1 (MQ=255)
                                     cGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGc                                                    <  1:4767006/93‑1 (MQ=255)
                                      gATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGcc                                                   <  1:5944715/93‑1 (MQ=255)
                                      gATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGcc                                                   >  1:1395980/1‑93 (MQ=255)
                                        tGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtg                                                 <  1:2804296/93‑1 (MQ=255)
                                         ggCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgt                                                <  1:5657422/93‑1 (MQ=255)
                                         ggCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTAGATCATCGCCtgt                                                >  1:7258277/1‑93 (MQ=255)
                                          gCCACATGGCGGTACCGTCCTGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgtt                                               <  2:2941876/93‑1 (MQ=255)
                                           ccccaGGGCGGTTCCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgttg                                              <  1:5472130/90‑1 (MQ=255)
                                           ccACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgttg                                              <  2:1618179/93‑1 (MQ=255)
                                           ccACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgttg                                              <  1:7735060/93‑1 (MQ=255)
                                             acaGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgttgtt                                            >  2:3525641/1‑93 (MQ=255)
                                               aGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgttgttgt                                          >  2:8408367/1‑93 (MQ=255)
                                                gggCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTa                                         <  2:7927765/93‑1 (MQ=255)
                                                gggCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTa                                         <  1:9309253/93‑1 (MQ=255)
                                                gggCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTa                                         >  1:4805338/1‑93 (MQ=255)
                                                gggCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTa                                         >  1:617420/1‑93 (MQ=255)
                                                  gCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTaca                                       <  2:5906668/93‑1 (MQ=255)
                                                  gCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTaca                                       <  2:3533913/93‑1 (MQ=255)
                                                   cGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTacac                                      >  1:2010275/1‑93 (MQ=255)
                                                   cGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTacac                                      <  1:3601037/93‑1 (MQ=255)
                                                    ggTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACg                                     >  2:852012/1‑93 (MQ=255)
                                                    ggTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACg                                     <  2:1231994/93‑1 (MQ=255)
                                                    ggTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACg                                     >  1:5965922/1‑93 (MQ=255)
                                                     gTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACgg                                    >  1:5148786/1‑93 (MQ=255)
                                                     gTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACgg                                    >  1:1766430/1‑93 (MQ=255)
                                                     gTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACgg                                    >  2:8191269/1‑93 (MQ=255)
                                                      tACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACggg                                   <  2:3393989/93‑1 (MQ=255)
                                                       tccGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGc                                  <  1:6395038/92‑1 (MQ=255)
                                                        ccGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGcc                                 <  2:3095667/93‑1 (MQ=255)
                                                        ccGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGcc                                 >  1:647953/1‑93 (MQ=255)
                                                         cGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCGGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGccc                                >  2:1883302/1‑93 (MQ=255)
                                                         cGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGccc                                >  2:5201942/1‑93 (MQ=255)
                                                         cGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGccc                                <  1:2487608/93‑1 (MQ=255)
                                                         cGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTAGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGccc                                <  1:1257016/93‑1 (MQ=255)
                                                          gTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCg                               >  1:6145734/1‑93 (MQ=255)
                                                           tCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCgg                              >  2:5323888/1‑93 (MQ=255)
                                                            ccGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGc                             <  2:8036383/93‑1 (MQ=255)
                                                             cGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCt                            <  2:1181990/93‑1 (MQ=255)
                                                             cGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGGTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCt                            >  2:1455445/1‑93 (MQ=255)
                                                               gCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCtgg                          >  1:3501297/1‑93 (MQ=255)
                                                                cTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCtggt                         <  1:9882148/93‑1 (MQ=255)
                                                                 ttGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCtggtg                        <  2:3196999/93‑1 (MQ=255)
                                                                 ttGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCtggtg                        <  2:4796106/93‑1 (MQ=255)
                                                                   gACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTTTTGTTGTACACGGGCCCGGCTGGTggg                      >  1:1038529/1‑93 (MQ=255)
                                                                     ccTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGt                    >  1:3909404/1‑93 (MQ=255)
                                                                     ccTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGt                    <  1:684596/93‑1 (MQ=255)
                                                                     ccTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGt                    <  1:7880677/93‑1 (MQ=255)
                                                                      cTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTgggggt                   >  2:6058931/1‑91 (MQ=255)
                                                                      cTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGtt                   <  1:7794644/93‑1 (MQ=255)
                                                                      cTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGtt                   >  1:1616875/1‑93 (MQ=255)
                                                                       tGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTa                  >  1:8988356/1‑93 (MQ=255)
                                                                         gAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTTCt                >  1:1702202/1‑93 (MQ=255)
                                                                         gAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACt                >  2:8860639/1‑93 (MQ=255)
                                                                         gAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACt                <  2:9569705/93‑1 (MQ=255)
                                                                          aaaaaGACCTTGTTTGCGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTa               <  1:2147901/93‑1 (MQ=255)
                                                                           aaaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTaa              >  1:1208145/1‑93 (MQ=255)
                                                                           aaaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTaa              <  2:4458366/93‑1 (MQ=255)
                                                                           aaaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTaa              <  1:6120881/93‑1 (MQ=255)
                                                                           aaaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTaa              <  1:8684728/93‑1 (MQ=255)
                                                                            aaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAt             <  2:6770431/93‑1 (MQ=255)
                                                                            aaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAt             <  1:9529429/93‑1 (MQ=255)
                                                                            aaaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAt             <  1:8421285/93‑1 (MQ=255)
                                                                             aaGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATg            <  2:6540673/93‑1 (MQ=255)
                                                                                 ccGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGGTACTAATGTTaa        >  1:38718/1‑93 (MQ=255)
                                                                                  cGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAAc       <  2:7016970/93‑1 (MQ=255)
                                                                                   gtgtTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAAcc      >  2:6117960/1‑93 (MQ=255)
                                                                                    tgtTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTACTGTTACCCg     >  1:6556468/1‑93 (MQ=255)
                                                                                    tgtTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCg     <  2:769692/93‑1 (MQ=255)
                                                                                    tgtTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCg     >  1:3065565/1‑93 (MQ=255)
                                                                                    tgtTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCg     >  2:2529085/1‑93 (MQ=255)
                                                                                      tttACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAg   <  2:6650163/93‑1 (MQ=255)
                                                                                      tttACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAg   >  2:9105585/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGG  >  NC_000913/1566451‑1566630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  >  NC_000913/1566448‑1566634
                                                                                             |                                                                                             
CAATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTAC                                                                                               >  SRR4302540.8497003/1‑94 (MQ=60)
CAATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTAC                                                                                               <  SRR4302540.7395939/94‑1 (MQ=60)
 AATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACG                                                                                              >  SRR4302540.4537932/1‑94 (MQ=60)
 AATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACG                                                                                              >  SRR4302540.2681061/1‑94 (MQ=60)
 AATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACG                                                                                              >  SRR4302540.7715499/1‑94 (MQ=60)
  ATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGC                                                                                             <  SRR4302540.8544193/94‑1 (MQ=60)
     TTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGA                                                                                          <  SRR4302540.815720/94‑1 (MQ=60)
       ACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGA                                                                                        >  SRR4302540.1423557/1‑94 (MQ=60)
        CCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGAC                                                                                       >  SRR4302540.4787846/1‑94 (MQ=60)
        CCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGAC                                                                                       <  SRR4302540.140508/94‑1 (MQ=60)
        CCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGAC                                                                                       <  SRR4302540.140509/94‑1 (MQ=60)
         CGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACG                                                                                      <  SRR4302540.5425786/94‑1 (MQ=60)
          GGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGACGAGACGT                                                                                     >  SRR4302540.2338315/1‑94 (MQ=60)
           GTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTT                                                                                    <  SRR4302540.5910271/94‑1 (MQ=60)
            TCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTG                                                                                   <  SRR4302540.10087732/94‑1 (MQ=60)
             CATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTTGAAAAAGACCGTGTTTACGCCGAGACGTTGC                                                                                  >  SRR4302540.3866978/1‑94 (MQ=60)
             CATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAACAAGACCGTGTTTACGCCGAGAGGTGGC                                                                                  >  SRR4302540.2668509/1‑94 (MQ=60)
              ATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCA                                                                                 <  SRR4302540.39209/94‑1 (MQ=60)
              ATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCA                                                                                 >  SRR4302540.6974048/1‑94 (MQ=60)
               TAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAG                                                                                >  SRR4302540.1216439/1‑94 (MQ=60)
                 AGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGAT                                                                              <  SRR4302540.3402530/94‑1 (MQ=60)
                 AGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGAT                                                                              <  SRR4302540.1974240/94‑1 (MQ=60)
                 AGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGAT                                                                              <  SRR4302540.1392567/94‑1 (MQ=60)
                     CGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATC                                                                          >  SRR4302540.3706702/1‑94 (MQ=60)
                     CGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATC                                                                          <  SRR4302540.9347692/94‑1 (MQ=60)
                      GGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCC                                                                         >  SRR4302540.7922868/1‑94 (MQ=60)
                      GGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCC                                                                         <  SRR4302540.693578/94‑1 (MQ=60)
                      GGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCC                                                                         >  SRR4302540.7700084/1‑94 (MQ=60)
                       GACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCA                                                                        <  SRR4302540.6022812/94‑1 (MQ=60)
                        ACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAG                                                                       <  SRR4302540.134617/94‑1 (MQ=60)
                        ACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAG                                                                       <  SRR4302540.6896709/94‑1 (MQ=60)
                         CCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGT                                                                      <  SRR4302540.8014384/94‑1 (MQ=60)
                          CACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTT                                                                     <  SRR4302540.6858432/94‑1 (MQ=60)
                           CCGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTT                                                                    <  SRR4302540.5997005/94‑1 (MQ=60)
                           ACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTT                                                                    >  SRR4302540.3557789/1‑94 (MQ=60)
                           ACGGCAAAATTTGCGATGGCCACAGGGCGGTACTCTCCGGCTTGACCTGGAAAAAGCCCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTT                                                                    <  SRR4302540.6494004/94‑1 (MQ=60)
                            CGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTG                                                                   >  SRR4302540.4414559/1‑94 (MQ=60)
                            CGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTG                                                                   >  SRR4302540.5866593/1‑94 (MQ=60)
                            CGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTG                                                                   >  SRR4302540.3855212/1‑94 (MQ=60)
                             GGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGT                                                                  >  SRR4302540.9891267/1‑94 (MQ=60)
                                  AATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATC                                                             <  SRR4302540.1950743/94‑1 (MQ=60)
                                   ATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTgcatct                                                            >  SRR4302540.3322185/1‑88 (MQ=60)
                                   ATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCA                                                            <  SRR4302540.7436410/94‑1 (MQ=60)
                                    TTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGTTCCAGTTTGTCGATCAT                                                           >  SRR4302540.7502487/1‑94 (MQ=60)
                                    TTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCAT                                                           <  SRR4302540.4672182/94‑1 (MQ=60)
                                     TTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATC                                                          <  SRR4302540.7350988/94‑1 (MQ=60)
                                     TTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATC                                                          <  SRR4302540.7350987/94‑1 (MQ=60)
                                     TTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATC                                                          <  SRR4302540.8259493/94‑1 (MQ=60)
                                      TTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCG                                                         <  SRR4302540.4428212/94‑1 (MQ=60)
                                       TCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGC                                                        >  SRR4302540.4375529/1‑94 (MQ=60)
                                        CGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCC                                                       >  SRR4302540.1410953/1‑94 (MQ=60)
                                        CGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCC                                                       <  SRR4302540.4817913/94‑1 (MQ=60)
                                         GATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCT                                                      <  SRR4302540.9397187/94‑1 (MQ=60)
                                         GATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCT                                                      <  SRR4302540.6010430/94‑1 (MQ=60)
                                          ATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTG                                                     >  SRR4302540.3535377/1‑94 (MQ=60)
                                          ATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTG                                                     >  SRR4302540.4824521/1‑94 (MQ=60)
                                          ATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTG                                                     <  SRR4302540.2513071/94‑1 (MQ=60)
                                           TGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGT                                                    <  SRR4302540.2834582/94‑1 (MQ=60)
                                           TGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTAGATCATCGCCTGT                                                    >  SRR4302540.7339263/1‑94 (MQ=60)
                                            GGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTT                                                   <  SRR4302540.1618194/94‑1 (MQ=60)
                                            GGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTT                                                   <  SRR4302540.5719716/94‑1 (MQ=60)
                                             GCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTG                                                  <  SRR4302540.6271798/94‑1 (MQ=60)
                                              CCCCAGGGCGGTTCCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGT                                                 <  SRR4302540.5532367/94‑1 (MQ=60)
                                              CCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGT                                                 <  SRR4302540.7825596/94‑1 (MQ=60)
                                              CCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGT                                                 <  SRR4302540.5532624/94‑1 (MQ=60)
                                              CCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGT                                                 <  SRR4302540.7825364/94‑1 (MQ=60)
                                                ACAGGGGGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTG                                               <  SRR4302540.9846782/94‑1 (MQ=60)
                                                 CAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGT                                              <  SRR4302540.5207181/94‑1 (MQ=60)
                                                  AGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTA                                             >  SRR4302540.4856769/1‑94 (MQ=60)
                                                  AGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTA                                             >  SRR4302540.624003/1‑94 (MQ=60)
                                                  AGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTA                                             >  SRR4302540.1785483/1‑94 (MQ=60)
                                                   GGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTAC                                            >  SRR4302540.2606056/1‑94 (MQ=60)
                                                   GGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTAC                                            <  SRR4302540.9420394/94‑1 (MQ=60)
                                                   GGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTAC                                            >  SRR4302540.10091597/1‑94 (MQ=60)
                                                     GCGGTACCGTCCGGCTTGACCTGGCAAAAGACCGTGTTTACGCCGCGACGTTGCAGATGATGCAGTTTGTCGCTTATCGCCTGCTCTTCTACAC                                          >  SRR4302540.9010015/1‑94 (MQ=60)
                                                     GCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACAC                                          >  SRR4302540.2032014/1‑94 (MQ=60)
                                                     GCGGTACCGGCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACAC                                          <  SRR4302540.5631674/94‑1 (MQ=60)
                                                      CGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACG                                         >  SRR4302540.6031934/1‑94 (MQ=60)
                                                      CGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACG                                         <  SRR4302540.3640387/94‑1 (MQ=60)
                                                      CGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACG                                         >  SRR4302540.5487761/1‑94 (MQ=60)
                                                       GGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTGCGCCGAGACGTTGCAGATGATCCAGTTTGTCGATAATCGCCTGTTGATGTACAGGG                                        >  SRR4302540.1241618/1‑94 (MQ=60)
                                                       GGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGG                                        >  SRR4302540.3140674/1‑94 (MQ=60)
                                                       GGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGG                                        >  SRR4302540.1785591/1‑94 (MQ=60)
                                                       GGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGG                                        >  SRR4302540.5204605/1‑94 (MQ=60)
                                                         TACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGC                                      <  SRR4302540.10006257/94‑1 (MQ=60)
                                                         TACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGC                                      <  SRR4302540.6237457/94‑1 (MQ=60)
                                                          TCCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCC                                     <  SRR4302540.6466221/94‑1 (MQ=60)
                                                          ACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCC                                     >  SRR4302540.654854/1‑94 (MQ=60)
                                                          ACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCC                                     <  SRR4302540.6466222/94‑1 (MQ=60)
                                                           CCGTGCGGCTTGACCTGGAAAAAGGCCATGTTTTCGCCGAGACGTTGCGGATGATCCAGTTTGTCGTTCATCGCGTGTTGTTGTACACGGGCCC                                    <  SRR4302540.4230839/94‑1 (MQ=60)
                                                           CCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCC                                    >  SRR4302540.6723729/1‑94 (MQ=60)
                                                            CGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCG                                   >  SRR4302540.6214187/1‑94 (MQ=60)
                                                            CGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCG                                   >  SRR4302540.6214184/1‑94 (MQ=60)
                                                            CGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCG                                   <  SRR4302540.2514403/94‑1 (MQ=60)
                                                            CGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCG                                   >  SRR4302540.4640234/1‑94 (MQ=60)
                                                            CGTCCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTAGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCG                                   <  SRR4302540.1269900/94‑1 (MQ=60)
                                                               CCGGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCT                                <  SRR4302540.2037458/94‑1 (MQ=60)
                                                                 GGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGG                              >  SRR4302540.2188786/1‑94 (MQ=60)
                                                                 GGCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGG                              >  SRR4302540.3539429/1‑94 (MQ=60)
                                                                  GCTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGT                             >  SRR4302540.510782/1‑94 (MQ=60)
                                                                   CTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGTGCCCGGCTGGTG                            >  SRR4302540.8820178/1‑94 (MQ=60)
                                                                   CTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTG                            <  SRR4302540.9997538/94‑1 (MQ=60)
                                                                   CTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTG                            <  SRR4302540.8483257/94‑1 (MQ=60)
                                                                   CTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTG                            <  SRR4302540.2903456/94‑1 (MQ=60)
                                                                   CTTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTG                            <  SRR4302540.9997534/94‑1 (MQ=60)
                                                                    TTGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGG                           >  SRR4302540.488634/1‑94 (MQ=60)
                                                                     TGACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTTTTGTTGTACACGGGCCCGGCTGGTGGG                          >  SRR4302540.1049090/1‑94 (MQ=60)
                                                                       ACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGT                        >  SRR4302540.3951775/1‑94 (MQ=60)
                                                                       ACCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGT                        <  SRR4302540.988079/94‑1 (MQ=60)
                                                                        CCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTT                       >  SRR4302540.1634610/1‑94 (MQ=60)
                                                                        CCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTT                       >  SRR4302540.7856187/1‑94 (MQ=60)
                                                                        CCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTT                       <  SRR4302540.691942/94‑1 (MQ=60)
                                                                        CCTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTT                       <  SRR4302540.7972704/94‑1 (MQ=60)
                                                                         CTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTA                      >  SRR4302540.9095846/1‑94 (MQ=60)
                                                                         CTGGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTA                      <  SRR4302540.7885753/94‑1 (MQ=60)
                                                                           GGAAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTTCT                    >  SRR4302540.1720628/1‑94 (MQ=60)
                                                                             AAAAAGACCTTGTTTGCGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAA                  <  SRR4302540.2170997/94‑1 (MQ=60)
                                                                             AAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAA                  >  SRR4302540.9513490/1‑94 (MQ=60)
                                                                             AAAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAA                  >  SRR4302540.1220543/1‑94 (MQ=60)
                                                                              AAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAT                 <  SRR4302540.6189052/94‑1 (MQ=60)
                                                                              AAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAT                 <  SRR4302540.6189051/94‑1 (MQ=60)
                                                                              AAAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAAT                 <  SRR4302540.8785576/94‑1 (MQ=60)
                                                                               AAAGACCGTGTTTACGCCGAGGCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATG                <  SRR4302540.8519035/94‑1 (MQ=60)
                                                                               AAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATG                <  SRR4302540.9642252/94‑1 (MQ=60)
                                                                               AAAGACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATG                <  SRR4302540.8519036/94‑1 (MQ=60)
                                                                                   ACCGTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGGTACTAATGTTAA            >  SRR4302540.39114/1‑94 (MQ=60)
                                                                                      GTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTACTGTTACCCG         >  SRR4302540.6629169/1‑94 (MQ=60)
                                                                                      GTGTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCG         >  SRR4302540.3099195/1‑94 (MQ=60)
                                                                                        GTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAG       >  SRR4302540.1689852/1‑94 (MQ=60)
                                                                                        GTTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGAGGGGTTACTAATGTCAACCGAG       >  SRR4302540.3567093/1‑94 (MQ=60)
                                                                                         TTTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGG      >  SRR4302540.8624371/1‑94 (MQ=60)
                                                                                          TTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGA     <  SRR4302540.8232245/94‑1 (MQ=60)
                                                                                          TTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGA     <  SRR4302540.615540/94‑1 (MQ=60)
                                                                                          TTACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCACAGCCTGTTGTTGTACACGCGCACGGCTGGTGGGGTTACTAATGTTAACCGAGGA     <  SRR4302540.6566017/94‑1 (MQ=60)
                                                                                           TACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAA    <  SRR4302540.7600123/94‑1 (MQ=60)
                                                                                           TACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAA    <  SRR4302540.929231/94‑1 (MQ=60)
                                                                                            ACGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAA   >  SRR4302540.5824591/1‑94 (MQ=60)
                                                                                             TGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGGGGGGTTACTAATGTTAACCGAGGACAC  >  SRR4302540.5090578/1‑94 (MQ=60)
                                                                                             CGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  <  SRR4302540.9560074/94‑1 (MQ=60)
                                                                                             CGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  >  SRR4302540.1273504/1‑94 (MQ=60)
                                                                                             CGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  <  SRR4302540.504988/94‑1 (MQ=60)
                                                                                             CGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  <  SRR4302540.7686766/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAC  >  NC_000913/1566448‑1566634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑