Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,731,175 T→C R1355R (CGT→CGC)  lhr → Lhr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,731,1750TC99.1% 707.1 / ‑3.4 227R1355R (CGT→CGC) lhrLhr
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (107/116);  total (109/118)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 240 total aligned reads displayed.

GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG  >  NC_000913/1731085‑1731264
                                                                                          |                                                                                         
gTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCa                                                                                         <  2:9452857/93‑1 (MQ=255)
    ttATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCg                                                                                     >  2:335718/1‑93 (MQ=255)
    ttATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCg                                                                                     >  2:6702043/1‑93 (MQ=255)
    ttATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCg                                                                                     >  1:2635107/1‑93 (MQ=255)
    ttATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCg                                                                                     <  2:3549481/93‑1 (MQ=255)
     tATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCgg                                                                                    <  1:946736/93‑1 (MQ=255)
      aTTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCggg                                                                                   >  1:1588390/1‑92 (MQ=255)
       ttGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTg                                                                                  >  2:1799891/1‑93 (MQ=255)
        tGCAGGTGGAGCCGCTAAACGATACCGAAAGGATGCTGGCGCTGGCGTAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGa                                                                                 <  1:1105831/93‑1 (MQ=255)
        tGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGGGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGa                                                                                 <  1:4712976/93‑1 (MQ=255)
        tGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGa                                                                                 <  1:9756627/93‑1 (MQ=255)
         gCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAt                                                                                <  2:1938049/93‑1 (MQ=255)
          gaGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATa                                                                               <  1:8237991/92‑1 (MQ=255)
            ggTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGc                                                                             >  1:6218070/1‑93 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            <  2:2150760/93‑1 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            <  2:8824533/93‑1 (MQ=255)
              tGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCg                                                                           >  1:505724/1‑93 (MQ=255)
               ggAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCTGa                                                                          <  2:6281415/93‑1 (MQ=255)
                gAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGaa                                                                         >  2:8411732/1‑93 (MQ=255)
                  gCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGaaaa                                                                       <  1:9951141/93‑1 (MQ=255)
                    taCTAAACGATACCGAATGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGTCGAAAata                                                                     >  2:8970419/2‑93 (MQ=255)
                    cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAata                                                                     <  2:302710/93‑1 (MQ=255)
                     aCTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAatat                                                                    >  2:6721013/1‑93 (MQ=255)
                     aCTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAatat                                                                    >  1:33853/1‑93 (MQ=255)
                     aCTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCGACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAatat                                                                    <  2:3995825/93‑1 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  <  1:2417517/93‑1 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  2:3015163/1‑93 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  1:327677/1‑93 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  1:9021960/1‑93 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  1:798681/1‑93 (MQ=255)
                        aaaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATccc                                                                 <  2:2356494/93‑1 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                <  2:8159407/93‑1 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                <  2:713297/93‑1 (MQ=255)
                          aCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTg                                                               <  1:10047193/93‑1 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             >  2:9928374/1‑93 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             >  2:9175569/1‑93 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             <  1:4660065/93‑1 (MQ=255)
                              tACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCgg                                                           >  2:8025373/1‑93 (MQ=255)
                                cgtaaaGGATGCTGGCGCTGGCGCAAAATATGCTCGACCGCGACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGt                                                         <  1:687358/90‑1 (MQ=255)
                                 cGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATCTCCCTGGCGCGtt                                                        >  1:7905335/1‑93 (MQ=255)
                                 cGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCggggt                                                        >  2:101784/1‑91 (MQ=255)
                                  gAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGttt                                                       >  1:7506527/1‑93 (MQ=255)
                                   aaaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCCGGCGGTGTTAGCCGAAAATATCCCTGGCGGGTTTc                                                      >  2:7717474/1‑93 (MQ=255)
                                   aaaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTc                                                      <  1:1607243/93‑1 (MQ=255)
                                   aaaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTc                                                      >  1:5533426/1‑93 (MQ=255)
                                    aaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTcc                                                     <  2:8366650/93‑1 (MQ=255)
                                     aGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCa                                                    <  1:3955055/93‑1 (MQ=255)
                                      ggATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCAt                                                   <  2:5663574/93‑1 (MQ=255)
                                      ggATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCAt                                                   <  1:3888400/93‑1 (MQ=255)
                                       gATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATc                                                  <  2:3832013/93‑1 (MQ=255)
                                       gATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATc                                                  <  1:9163051/93‑1 (MQ=255)
                                        aTGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCg                                                 <  1:5977060/93‑1 (MQ=255)
                                         tGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGa                                                <  2:7859436/93‑1 (MQ=255)
                                         tGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGa                                                <  2:7671629/93‑1 (MQ=255)
                                            tggcgctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGc                                             <  1:7079459/93‑1 (MQ=255)
                                              gcgctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCaa                                           >  2:9084794/1‑93 (MQ=255)
                                                gctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGGCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAc                                         <  1:4847858/93‑1 (MQ=255)
                                                gctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAc                                         >  2:7130942/1‑93 (MQ=255)
                                                gctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGACAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAc                                         <  2:660024/93‑1 (MQ=255)
                                                   ggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCt                                      <  2:4939680/93‑1 (MQ=255)
                                                   ggcgGAAAATATGCTCCACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCt                                      >  1:6185792/1‑93 (MQ=255)
                                                    gcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCtt                                     <  2:3744667/93‑1 (MQ=255)
                                                      ggAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTg                                   <  1:5591811/93‑1 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACTCTTTGTc                                 <  2:2816280/93‑1 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTc                                 >  2:5336207/1‑93 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTc                                 <  1:8443807/93‑1 (MQ=255)
                                                           atatGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGaa                              >  2:1244842/1‑93 (MQ=255)
                                                           atatGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGaa                              <  1:428030/93‑1 (MQ=255)
                                                           atatGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGaa                              >  2:4482047/1‑93 (MQ=255)
                                                              tGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTa                           <  2:5913399/93‑1 (MQ=255)
                                                              tGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTa                           <  2:9711748/93‑1 (MQ=255)
                                                              tGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTa                           <  1:8896524/93‑1 (MQ=255)
                                                                  cGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGa                       <  2:5952335/93‑1 (MQ=255)
                                                                   gACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGaa                      >  1:2257780/1‑93 (MQ=255)
                                                                     ccGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGa                    <  2:564579/93‑1 (MQ=255)
                                                                      cGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGAc                   <  2:7279988/93‑1 (MQ=255)
                                                                      cGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGAc                   <  1:179937/93‑1 (MQ=255)
                                                                        cTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTc                 >  1:2673113/1‑93 (MQ=255)
                                                                        cTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTc                 <  1:7704368/93‑1 (MQ=255)
                                                                        cTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTc                 >  2:7522306/1‑93 (MQ=255)
                                                                         tACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTcc                <  2:3543803/93‑1 (MQ=255)
                                                                         tACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTcc                >  1:8134415/1‑93 (MQ=255)
                                                                          aCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCg               >  1:6503075/1‑93 (MQ=255)
                                                                             gCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGc            >  1:4509231/1‑93 (MQ=255)
                                                                                tcatcaGTCGCCAGGCGGTGATAGGCGAAAATATCCCTGGCGGGTTTCCATCGATGCAACCGCTTTGTCGACGTATCGAAGACTCCGGGAGaa         >  1:1275044/1‑93 (MQ=255)
                                                                                tcatcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGag         >  1:862274/1‑92 (MQ=255)
                                                                                tcatcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGaa         >  1:750607/1‑93 (MQ=255)
                                                                                 catcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAAt        >  2:2638980/1‑93 (MQ=255)
                                                                                  atcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAAtt       <  1:6562894/93‑1 (MQ=255)
                                                                                  atcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCGTTGTCGAAGTATGGAAGACTCCGGGCGAAtt       <  2:4568470/93‑1 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      <  1:4204946/93‑1 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      <  1:3735311/93‑1 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      <  1:8397999/93‑1 (MQ=255)
                                                                                     aGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATg    >  2:4388153/1‑93 (MQ=255)
                                                                                     aGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATg    >  2:8572774/1‑93 (MQ=255)
                                                                                      gtcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGc   <  1:2064379/93‑1 (MQ=255)
                                                                                      gtcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGc   <  1:2894082/93‑1 (MQ=255)
                                                                                      gtcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGc   >  1:3526260/1‑93 (MQ=255)
                                                                                      gtcgccAGGCGGTGATAGCCGAAAATATACCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGc   >  1:5247201/1‑93 (MQ=255)
                                                                                       tcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCg  <  2:2392698/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG  >  NC_000913/1731085‑1731264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  >  NC_000913/1731082‑1731268
                                                                                             |                                                                                             
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGC                                                                                               <  SRR4302540.2238821/94‑1 (MQ=60)
 TGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCC                                                                                              >  SRR4302540.7701356/1‑94 (MQ=60)
  GGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCA                                                                                             <  SRR4302540.7394420/94‑1 (MQ=60)
   GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAG                                                                                            <  SRR4302540.577359/94‑1 (MQ=60)
   GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAG                                                                                            <  SRR4302540.1716761/94‑1 (MQ=60)
      GTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCG                                                                                         >  SRR4302540.2663640/1‑94 (MQ=60)
        TATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGT                                                                                       <  SRR4302540.8158824/94‑1 (MQ=60)
        TATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGT                                                                                       <  SRR4302540.956600/94‑1 (MQ=60)
        TATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGG                                                                                       >  SRR4302540.1605744/1‑94 (MQ=60)
           TGCAGGTGGAGCCGCTAAACGATACCGAAAGGATGCTGGCGCTGGCGTAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAT                                                                                    <  SRR4302540.1117238/94‑1 (MQ=60)
           TGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGGGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAT                                                                                    <  SRR4302540.4763336/94‑1 (MQ=60)
           TGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAT                                                                                    <  SRR4302540.8358127/94‑1 (MQ=60)
           TGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAT                                                                                    <  SRR4302540.9871085/94‑1 (MQ=60)
             GAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAG                                                                                  <  SRR4302540.8333731/94‑1 (MQ=60)
             CAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAG                                                                                  <  SRR4302540.8515343/94‑1 (MQ=60)
             CAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAG                                                                                  <  SRR4302540.4200289/94‑1 (MQ=60)
             CAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAG                                                                                  <  SRR4302540.1424247/94‑1 (MQ=60)
              AGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGC                                                                                 >  SRR4302540.9322897/1‑94 (MQ=60)
              AGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGC                                                                                 >  SRR4302540.6287251/1‑94 (MQ=60)
               GGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCC                                                                                <  SRR4302540.564998/94‑1 (MQ=60)
                GTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCG                                                                               >  SRR4302540.511139/1‑94 (MQ=60)
                 GGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGA                                                                              <  SRR4302540.5262127/94‑1 (MQ=60)
                  GGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTTGCAAGGCGGTGATAGCCGCA                                                                             >  SRR4302540.3721191/1‑94 (MQ=60)
                  GGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAA                                                                             <  SRR4302540.3934213/94‑1 (MQ=60)
                     GCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAAT                                                                          <  SRR4302540.10067314/94‑1 (MQ=60)
                      CCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATA                                                                         >  SRR4302540.4532533/1‑94 (MQ=60)
                       CACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATAT                                                                        >  SRR4302540.2299841/1‑94 (MQ=60)
                       CACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATAT                                                                        >  SRR4302540.34212/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302540.807162/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302540.9130219/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302540.10098523/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302540.330957/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302540.1663616/1‑94 (MQ=60)
                          TAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCC                                                                     <  SRR4302540.7498203/94‑1 (MQ=60)
                          TAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCC                                                                     <  SRR4302540.2443568/94‑1 (MQ=60)
                          TAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCC                                                                     >  SRR4302540.5009610/1‑94 (MQ=60)
                          TAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCC                                                                     >  SRR4302540.9519269/1‑94 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    >  SRR4302540.9943568/1‑94 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    <  SRR4302540.7105246/94‑1 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    <  SRR4302540.4767870/94‑1 (MQ=60)
                            AACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTG                                                                   <  SRR4302540.7749488/94‑1 (MQ=60)
                            AACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTG                                                                   <  SRR4302540.3493263/94‑1 (MQ=60)
                             ACGATTCCGAAAGGATGCTGGCGCTGACGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGG                                                                  <  SRR4302540.4350293/94‑1 (MQ=60)
                             ACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGG                                                                  <  SRR4302540.10163988/94‑1 (MQ=60)
                              CGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGC                                                                 <  SRR4302540.4893832/94‑1 (MQ=60)
                               GATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCG                                                                >  SRR4302540.3280706/1‑94 (MQ=60)
                               GATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCG                                                                <  SRR4302540.4709944/94‑1 (MQ=60)
                               GATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCG                                                                >  SRR4302540.2689179/1‑94 (MQ=60)
                                 TACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGG                                                              >  SRR4302540.9883985/1‑94 (MQ=60)
                                   cgtAAAGGATGCTGGCGCTGGCGCAAAATATGCTCGACCGCGACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTT                                                            <  SRR4302540.694736/91‑1 (MQ=60)
                                   CCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATCTCCCTGGCGCGTT                                                            >  SRR4302540.7997527/1‑94 (MQ=60)
                                   CCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTCGCGGGAT                                                            >  SRR4302540.3842334/1‑94 (MQ=60)
                                    CGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTT                                                           >  SRR4302540.7590425/1‑94 (MQ=60)
                                    CGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTT                                                           >  SRR4302540.8911978/1‑94 (MQ=60)
                                    CGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTT                                                           >  SRR4302540.7590424/1‑94 (MQ=60)
                                    CGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTT                                                           >  SRR4302540.6709425/1‑94 (MQ=60)
                                     GAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTC                                                          >  SRR4302540.5594359/1‑94 (MQ=60)
                                     GAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTC                                                          <  SRR4302540.8541598/94‑1 (MQ=60)
                                      AAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCC                                                         <  SRR4302540.1624837/94‑1 (MQ=60)
                                      AAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCC                                                         <  SRR4302540.1624836/94‑1 (MQ=60)
                                        AGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCCT                                                       >  SRR4302540.9645785/1‑94 (MQ=60)
                                        AGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCAT                                                       <  SRR4302540.3997739/94‑1 (MQ=60)
                                         GGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATC                                                      <  SRR4302540.3930624/94‑1 (MQ=60)
                                          GATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCG                                                     <  SRR4302540.9273013/94‑1 (MQ=60)
                                           ATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGA                                                    <  SRR4302540.6043224/94‑1 (MQ=60)
                                           ATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGA                                                    <  SRR4302540.6043225/94‑1 (MQ=60)
                                           ATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGA                                                    >  SRR4302540.4188131/1‑94 (MQ=60)
                                            TGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGAT                                                   >  SRR4302540.1153320/1‑94 (MQ=60)
                                               TGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCA                                                <  SRR4302540.7158252/94‑1 (MQ=60)
                                                   GCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGGCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACG                                            <  SRR4302540.4899796/94‑1 (MQ=60)
                                                   GCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACG                                            >  SRR4302540.9140642/1‑94 (MQ=60)
                                                    CTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCAATCGATGCAAACGC                                           >  SRR4302540.2679242/1‑94 (MQ=60)
                                                     TGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCT                                          >  SRR4302540.4727040/1‑94 (MQ=60)
                                                     TGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCT                                          >  SRR4302540.6254684/1‑94 (MQ=60)
                                                     TGGCGGAAAATATGCTCCACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCT                                          >  SRR4302540.6254683/1‑94 (MQ=60)
                                                      GGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTT                                         >  SRR4302540.3403486/1‑94 (MQ=60)
                                                        CGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTG                                       >  SRR4302540.240761/1‑94 (MQ=60)
                                                        CGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTG                                       <  SRR4302540.512047/94‑1 (MQ=60)
                                                         GGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGT                                      <  SRR4302540.5653336/94‑1 (MQ=60)
                                                          GAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGCTAGCCGAAAATATCCCTGGCGGGTTTCCCTCGCTTCAAACGCTTTTTC                                     >  SRR4302540.2575931/1‑94 (MQ=60)
                                                           AAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCG                                    <  SRR4302540.3331846/94‑1 (MQ=60)
                                                           AAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCG                                    <  SRR4302540.8541826/94‑1 (MQ=60)
                                                           AAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCG                                    >  SRR4302540.6742032/1‑94 (MQ=60)
                                                              ATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAG                                 <  SRR4302540.432532/94‑1 (MQ=60)
                                                              ATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAG                                 >  SRR4302540.779887/1‑94 (MQ=60)
                                                              ATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAG                                 >  SRR4302540.8251144/1‑94 (MQ=60)
                                                              ATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAG                                 >  SRR4302540.8046287/1‑94 (MQ=60)
                                                               TATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGT                                >  SRR4302540.4270864/1‑94 (MQ=60)
                                                                 TGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTAT                              <  SRR4302540.8999508/94‑1 (MQ=60)
                                                                 TGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTAT                              <  SRR4302540.8580278/94‑1 (MQ=60)
                                                                   CTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGG                            <  SRR4302540.7880331/94‑1 (MQ=60)
                                                                   CTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGG                            <  SRR4302540.7386828/94‑1 (MQ=60)
                                                                     CGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAA                          >  SRR4302540.2282014/1‑94 (MQ=60)
                                                                      GACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAG                         >  SRR4302540.6076926/1‑94 (MQ=60)
                                                                         CGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACT                      <  SRR4302540.181728/94‑1 (MQ=60)
                                                                          GCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTC                     >  SRR4302540.2702058/1‑94 (MQ=60)
                                                                          GCTACGGCATCATCAGTAGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTC                     <  SRR4302540.9100787/94‑1 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCC                    <  SRR4302540.7792564/94‑1 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCC                    >  SRR4302540.8228825/1‑94 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCC                    >  SRR4302540.3678459/1‑94 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCC                    >  SRR4302540.1468747/1‑94 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGACGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTCTGGAAGACTCG                    >  SRR4302540.8228824/1‑94 (MQ=60)
                                                                            tcgggccgcatcatgaGTCAGGCGGTGATAGCCGAAAATAGCCGTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCG                   <  SRR4302540.312962/78‑1 (MQ=60)
                                                                            TACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCG                   >  SRR4302540.6575362/1‑94 (MQ=60)
                                                                             ACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGG                  >  SRR4302540.1104848/1‑94 (MQ=60)
                                                                               GGCATCATGAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGC                >  SRR4302540.6264708/1‑94 (MQ=60)
                                                                               GGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGC                >  SRR4302540.4558093/1‑94 (MQ=60)
                                                                                 CATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGA              >  SRR4302540.2752994/1‑94 (MQ=60)
                                                                                  ATCATCAGTCGCCAGGCGGTGATAGGCGAAAATATCCCTGGCGGGTTTCCATCGATGCAACCGCTTTGTCGACGTATCGAAGACTCCGGGAGAA             >  SRR4302540.1288185/1‑94 (MQ=60)
                                                                                  ATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAG             >  SRR4302540.871432/1‑94 (MQ=60)
                                                                                  ATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAA             >  SRR4302540.758598/1‑94 (MQ=60)
                                                                                   TCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAAT            <  SRR4302540.6438760/94‑1 (MQ=60)
                                                                                    CATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATT           >  SRR4302540.2253634/1‑94 (MQ=60)
                                                                                    CATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATT           >  SRR4302540.1836980/1‑94 (MQ=60)
                                                                                     ATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTA          >  SRR4302540.5417748/1‑94 (MQ=60)
                                                                                     ATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTA          <  SRR4302540.8734469/94‑1 (MQ=60)
                                                                                     ATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTA          <  SRR4302540.6635636/94‑1 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302540.4570555/94‑1 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302540.8495459/94‑1 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302540.6598854/94‑1 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302540.4250709/94‑1 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302540.3776066/94‑1 (MQ=60)
                                                                                        AGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGC       >  SRR4302540.3564716/1‑94 (MQ=60)
                                                                                        AGTCGCCAGGCGGTGATAGCCGAAAATATACCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGC       >  SRR4302540.5304434/1‑94 (MQ=60)
                                                                                         GTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG      <  SRR4302540.2086593/94‑1 (MQ=60)
                                                                                         GTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG      <  SRR4302540.2925637/94‑1 (MQ=60)
                                                                                            GCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGG   <  SRR4302540.271383/94‑1 (MQ=60)
                                                                                            GCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGG   >  SRR4302540.9442991/1‑94 (MQ=60)
                                                                                            GCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGG   >  SRR4302540.3404167/1‑94 (MQ=60)
                                                                                             CCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  <  SRR4302540.8690706/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  >  NC_000913/1731082‑1731268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑