Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,734,705 G→A L204L (CTG→TTG)  b1657 ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,734,7050GA99.5% 704.5 / NA 195L204L (CTG→TTG) b1657putative transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (93/101);  minor base T (0/1);  total (93/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTT  >  NC_000913/1734615‑1734793
                                                                                          |                                                                                        
cGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGacaaca                                                                                        >  1:2406903/1‑93 (MQ=255)
 gTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAacac                                                                                       >  1:5313702/1‑93 (MQ=255)
 gTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAacac                                                                                       <  1:3901259/93‑1 (MQ=255)
  tGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCCGCTCCCAGTACCGTCGTCAGCAATGCAGACAACAcc                                                                                      >  2:1478665/1‑93 (MQ=255)
  tGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACAcc                                                                                      >  2:5083211/1‑93 (MQ=255)
   gggTAATACTTTGCAGTACCGGAGAGATATGGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCt                                                                                     >  1:4629119/1‑93 (MQ=255)
   gggTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCt                                                                                     <  1:6205507/93‑1 (MQ=255)
    ggTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTg                                                                                    >  2:3330442/1‑93 (MQ=255)
     gTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGc                                                                                   <  1:5222973/93‑1 (MQ=255)
     gTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGc                                                                                   <  2:5476307/93‑1 (MQ=255)
     gTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGc                                                                                   <  1:4522780/93‑1 (MQ=255)
      tAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCg                                                                                  <  2:3687464/93‑1 (MQ=255)
       aaTACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCgg                                                                                 >  2:2572148/1‑93 (MQ=255)
        aTACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGa                                                                                <  2:5670775/93‑1 (MQ=255)
        aTACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGa                                                                                <  1:1282424/93‑1 (MQ=255)
         tACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAc                                                                               >  2:4003453/1‑93 (MQ=255)
         tACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAc                                                                               <  2:3848903/93‑1 (MQ=255)
         tACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACAATGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAc                                                                               >  1:3388062/1‑93 (MQ=255)
           cTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGc                                                                             <  2:4456932/93‑1 (MQ=255)
            tttGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCa                                                                            >  2:222933/1‑93 (MQ=255)
            tttGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCa                                                                            <  1:6021109/93‑1 (MQ=255)
            tttGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCa                                                                            <  1:3117985/93‑1 (MQ=255)
              tGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCAtt                                                                          <  1:4349421/93‑1 (MQ=255)
               gCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTa                                                                         >  2:3894862/1‑93 (MQ=255)
               gCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTa                                                                         >  1:1233013/1‑93 (MQ=255)
                   tACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATAc                                                                     <  2:5123354/93‑1 (MQ=255)
                   tACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATAc                                                                     >  2:5780439/1‑93 (MQ=255)
                      cGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGc                                                                  >  2:1531451/1‑93 (MQ=255)
                      cGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGc                                                                  >  1:2479100/1‑93 (MQ=255)
                       ggAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGcc                                                                 >  2:4631043/1‑93 (MQ=255)
                       ggAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGcc                                                                 <  2:5234458/93‑1 (MQ=255)
                           agaTATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGct                                                             >  1:4847910/1‑93 (MQ=255)
                              tataGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCtt                                                          >  2:74690/1‑93 (MQ=255)
                              tataGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCtt                                                          >  2:4073216/1‑93 (MQ=255)
                               ataGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCttt                                                         >  1:2891933/1‑93 (MQ=255)
                               ataGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCttt                                                         <  2:4732179/93‑1 (MQ=255)
                                taGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCtttt                                                        <  2:552168/93‑1 (MQ=255)
                                taGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCtttt                                                        <  2:72023/93‑1 (MQ=255)
                                 aGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACGCCTGCGGACGCATTAATACCGCCAGCTCttttt                                                       <  2:1026782/93‑1 (MQ=255)
                                 aGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCttttt                                                       <  2:6077354/93‑1 (MQ=255)
                                      tAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACt                                                  >  2:3004137/1‑93 (MQ=255)
                                       agagagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACtt                                                 >  2:4415768/1‑93 (MQ=255)
                                       agagagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACtt                                                 >  2:5194746/1‑93 (MQ=255)
                                       agagagTAAACACTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACtt                                                 <  1:154779/93‑1 (MQ=255)
                                        gagagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTc                                                >  1:2189340/1‑93 (MQ=255)
                                         agagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCa                                               <  1:3079909/93‑1 (MQ=255)
                                          gagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAg                                              >  2:4104685/1‑93 (MQ=255)
                                            gTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGt                                            <  1:3698864/93‑1 (MQ=255)
                                            gTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGt                                            >  2:23577/1‑93 (MQ=255)
                                            gTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGt                                            <  1:1386467/93‑1 (MQ=255)
                                             tAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTc                                           <  2:2689182/93‑1 (MQ=255)
                                                aCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTg                                        >  2:1796833/1‑93 (MQ=255)
                                                 cATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGc                                       <  2:4871610/93‑1 (MQ=255)
                                                  aTTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTATTTCACTTCAGGTCGTGcc                                      <  2:6166104/93‑1 (MQ=255)
                                                   ttGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGccc                                     >  1:5982387/1‑93 (MQ=255)
                                                     gCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCt                                   <  2:5972801/93‑1 (MQ=255)
                                                       aCCAGCTCCCAGTACCGTCGTCAGCAATGCACACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGc                                 >  2:5659363/1‑93 (MQ=255)
                                                         cAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGcac                               <  1:2837745/93‑1 (MQ=255)
                                                          aGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGcacc                              >  1:6107831/1‑93 (MQ=255)
                                                             tCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCaccacc                           >  2:5302062/1‑93 (MQ=255)
                                                             tCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCaccacc                           >  2:5269561/1‑93 (MQ=255)
                                                              cccAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCt                          >  1:73620/1‑93 (MQ=255)
                                                               ccAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCtt                         <  2:1365946/93‑1 (MQ=255)
                                                               ccAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCtt                         <  1:5474833/93‑1 (MQ=255)
                                                                cAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCttt                        >  1:1633996/1‑93 (MQ=255)
                                                                  gtgccGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAg                      <  1:2415656/90‑1 (MQ=255)
                                                                  gTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAg                      <  1:5787351/93‑1 (MQ=255)
                                                                   tACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAgg                     >  1:4276813/1‑93 (MQ=255)
                                                                    aCCGTCGTCAGCAATGCATACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCCCCACCTTTAGGt                    <  2:4678868/93‑1 (MQ=255)
                                                                     ccGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTa                   <  2:2390844/93‑1 (MQ=255)
                                                                      cgtcgtCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTaa                  <  1:5089554/93‑1 (MQ=255)
                                                                      cgtcgtCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTaa                  <  1:5524245/93‑1 (MQ=255)
                                                                       gtcgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAt                 >  1:4757411/1‑93 (MQ=255)
                                                                       gtcgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAt                 <  1:5699190/93‑1 (MQ=255)
                                                                        tcgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATg                <  2:3067285/93‑1 (MQ=255)
                                                                        tcgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATg                >  2:2823264/1‑93 (MQ=255)
                                                                        tcgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATg                <  1:1492461/93‑1 (MQ=255)
                                                                         cgtcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATga               <  2:1689753/93‑1 (MQ=255)
                                                                           tcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATgaga             >  1:3612280/1‑93 (MQ=255)
                                                                           tcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATgaga             >  1:5360891/1‑93 (MQ=255)
                                                                           tcAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATgaga             >  1:5448843/1‑93 (MQ=255)
                                                                            cAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaa            <  2:2106496/93‑1 (MQ=255)
                                                                              gCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaaga          >  2:3644957/1‑93 (MQ=255)
                                                                              gCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaaga          <  2:3383845/93‑1 (MQ=255)
                                                                              gCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaaga          <  2:1155662/93‑1 (MQ=255)
                                                                              gCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCACTGCACCACCTTTAGGTAATGagaaga          >  2:3945319/1‑93 (MQ=255)
                                                                               cAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAgaagaa         <  2:3542754/93‑1 (MQ=255)
                                                                                aaTGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAc        <  1:5035674/93‑1 (MQ=255)
                                                                                aaTGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAc        >  1:866071/1‑93 (MQ=255)
                                                                                 aTGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACa       <  1:4481905/93‑1 (MQ=255)
                                                                                  tGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAg      >  1:1132968/1‑93 (MQ=255)
                                                                                  tGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAg      >  1:671794/1‑93 (MQ=255)
                                                                                   gCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGa     <  1:3770830/93‑1 (MQ=255)
                                                                                   gCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGa     <  2:4490665/93‑1 (MQ=255)
                                                                                    cAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGAc    <  1:270742/93‑1 (MQ=255)
                                                                                    cAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCGTTAGGTCATGAGAAGAACAGAc    >  2:1073214/1‑93 (MQ=255)
                                                                                     aGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACt   <  1:2354094/93‑1 (MQ=255)
                                                                                     aGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACt   <  2:431280/93‑1 (MQ=255)
                                                                                     aGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACt   <  2:6257226/93‑1 (MQ=255)
                                                                                     aGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACt   >  1:1911413/1‑93 (MQ=255)
                                                                                          |                                                                                        
CGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTT  >  NC_000913/1734615‑1734793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  >  NC_000913/1734614‑1734797
                                                                                           |                                                                                            
GCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACA                                                                                            >  SRR4302533.2421770/1‑94 (MQ=60)
 CGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACAC                                                                                           >  SRR4302533.6161401/1‑94 (MQ=60)
 CGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACAC                                                                                           <  SRR4302533.4691830/94‑1 (MQ=60)
 CGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACAC                                                                                           >  SRR4302533.5345543/1‑94 (MQ=60)
  GTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACC                                                                                          <  SRR4302533.3925076/94‑1 (MQ=60)
  GTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACC                                                                                          >  SRR4302533.4638873/1‑94 (MQ=60)
   TGGGTAATACTTTGCAGTACCGGAGAGATATGGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCT                                                                                         >  SRR4302533.4657174/1‑94 (MQ=60)
    GGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTG                                                                                        <  SRR4302533.6242723/94‑1 (MQ=60)
     GGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGC                                                                                       <  SRR4302533.3645319/94‑1 (MQ=60)
     GGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGC                                                                                       <  SRR4302533.543684/94‑1 (MQ=60)
     GGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCACAGTACCGTCGTCAGCAATGCAGACAACACCTGC                                                                                       <  SRR4302533.3830849/94‑1 (MQ=60)
      GTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCG                                                                                      <  SRR4302533.5254396/94‑1 (MQ=60)
      GTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCG                                                                                      <  SRR4302533.4550160/94‑1 (MQ=60)
        AATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGA                                                                                    <  SRR4302533.6030068/94‑1 (MQ=60)
         ATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAC                                                                                   <  SRR4302533.1290142/94‑1 (MQ=60)
         ATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAC                                                                                   <  SRR4302533.2449414/94‑1 (MQ=60)
         ATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACAATGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGAC                                                                                   >  SRR4302533.3408982/1‑94 (MQ=60)
          TACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACG                                                                                  <  SRR4302533.3748624/94‑1 (MQ=60)
            CTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCA                                                                                >  SRR4302533.2763265/1‑94 (MQ=60)
            CTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCA                                                                                >  SRR4302533.834264/1‑94 (MQ=60)
             TTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCAT                                                                               <  SRR4302533.3137157/94‑1 (MQ=60)
             TTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCAT                                                                               <  SRR4302533.6057594/94‑1 (MQ=60)
             TTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCAT                                                                               <  SRR4302533.4304559/94‑1 (MQ=60)
               TGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTA                                                                             <  SRR4302533.4375643/94‑1 (MQ=60)
               TGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTA                                                                             >  SRR4302533.1240391/1‑94 (MQ=60)
                 CAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAAT                                                                           >  SRR4302533.1259117/1‑94 (MQ=60)
                   GTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATAC                                                                         <  SRR4302533.3109030/94‑1 (MQ=60)
                    TACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACC                                                                        <  SRR4302533.33451/94‑1 (MQ=60)
                      CCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGC                                                                      >  SRR4302533.2494389/1‑94 (MQ=60)
                       CGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCC                                                                     >  SRR4302533.3613708/1‑94 (MQ=60)
                           GAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCT                                                                 >  SRR4302533.4877290/1‑94 (MQ=60)
                            AGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTC                                                                >  SRR4302533.4204461/1‑94 (MQ=60)
                             GATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCT                                                               <  SRR4302533.1665844/94‑1 (MQ=60)
                               TATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTT                                                             >  SRR4302533.2219361/1‑94 (MQ=60)
                               TATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTT                                                             >  SRR4302533.2909711/1‑94 (MQ=60)
                                 TAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTT                                                           <  SRR4302533.1239001/94‑1 (MQ=60)
                                  AGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTT                                                          >  SRR4302533.5498603/1‑94 (MQ=60)
                                        AGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTC                                                    >  SRR4302533.2202828/1‑94 (MQ=60)
                                        AGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTC                                                    <  SRR4302533.2089974/94‑1 (MQ=60)
                                        AGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTC                                                    <  SRR4302533.2527957/94‑1 (MQ=60)
                                        AGAGAGTAAACACTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTC                                                    <  SRR4302533.155765/94‑1 (MQ=60)
                                          AGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAG                                                  <  SRR4302533.2907397/94‑1 (MQ=60)
                                          AGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAG                                                  <  SRR4302533.3098807/94‑1 (MQ=60)
                                             GTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTC                                               <  SRR4302533.3721580/94‑1 (MQ=60)
                                             GTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTC                                               <  SRR4302533.1394810/94‑1 (MQ=60)
                                             GTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTC                                               <  SRR4302533.5187716/94‑1 (MQ=60)
                                                AACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTG                                            <  SRR4302533.2425039/94‑1 (MQ=60)
                                                   ATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCC                                         >  SRR4302533.6018618/1‑94 (MQ=60)
                                                     TGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCT                                       <  SRR4302533.5541158/94‑1 (MQ=60)
                                                      GCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTG                                      <  SRR4302533.6090936/94‑1 (MQ=60)
                                                       CACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGA                                     >  SRR4302533.1641273/1‑94 (MQ=60)
                                                        ACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCA                                    >  SRR4302533.5562600/1‑94 (MQ=60)
                                                         CCAGATCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCAC                                   >  SRR4302533.3025924/1‑94 (MQ=60)
                                                          CAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACC                                  >  SRR4302533.6144706/1‑94 (MQ=60)
                                                          CAGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACC                                  <  SRR4302533.2855210/94‑1 (MQ=60)
                                                           AGCTCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCA                                 >  SRR4302533.449412/1‑94 (MQ=60)
                                                              TCCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCT                              >  SRR4302533.74093/1‑94 (MQ=60)
                                                               CCCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTT                             <  SRR4302533.1349184/94‑1 (MQ=60)
                                                                CCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTT                            >  SRR4302533.1643893/1‑94 (MQ=60)
                                                                CCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTT                            >  SRR4302533.1430161/1‑94 (MQ=60)
                                                                CCAGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTT                            <  SRR4302533.5507834/94‑1 (MQ=60)
                                                                  AGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAG                          >  SRR4302533.4457691/1‑94 (MQ=60)
                                                                  AGTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAG                          <  SRR4302533.4821567/94‑1 (MQ=60)
                                                                   GTGCCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGG                         <  SRR4302533.2430579/94‑1 (MQ=60)
                                                                   GTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGG                         <  SRR4302533.5822313/94‑1 (MQ=60)
                                                                   GTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGG                         <  SRR4302533.4909465/94‑1 (MQ=60)
                                                                   GTACCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGG                         >  SRR4302533.4302534/1‑94 (MQ=60)
                                                                      CCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAA                      <  SRR4302533.531712/94‑1 (MQ=60)
                                                                      CCGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAA                      >  SRR4302533.5106189/1‑94 (MQ=60)
                                                                       aggcaTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     >  SRR4302533.5815725/6‑94 (MQ=60)
                                                                       CGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     <  SRR4302533.5120305/94‑1 (MQ=60)
                                                                       CGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     <  SRR4302533.5557597/94‑1 (MQ=60)
                                                                       CGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     >  SRR4302533.4786277/1‑94 (MQ=60)
                                                                       CGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     >  SRR4302533.707945/1‑94 (MQ=60)
                                                                       CGTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT                     >  SRR4302533.1535852/1‑94 (MQ=60)
                                                                        GTCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATG                    <  SRR4302533.5733608/94‑1 (MQ=60)
                                                                         TCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGA                   >  SRR4302533.6273159/1‑94 (MQ=60)
                                                                         TCGTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGA                   <  SRR4302533.1501461/94‑1 (MQ=60)
                                                                           GTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGA                 >  SRR4302533.5393089/1‑94 (MQ=60)
                                                                           GTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGA                 >  SRR4302533.5481667/1‑94 (MQ=60)
                                                                           GTCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGA                 >  SRR4302533.3634512/1‑94 (MQ=60)
                                                                            TCAGCAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAA                >  SRR4302533.5383485/1‑94 (MQ=60)
                                                                                CAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAC            >  SRR4302533.871143/1‑94 (MQ=60)
                                                                                CAATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAC            <  SRR4302533.2414169/94‑1 (MQ=60)
                                                                                 AATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACA           <  SRR4302533.5066174/94‑1 (MQ=60)
                                                                                  ATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAG          >  SRR4302533.675760/1‑94 (MQ=60)
                                                                                  ATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAG          <  SRR4302533.5832656/94‑1 (MQ=60)
                                                                                  ATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAG          >  SRR4302533.1139703/1‑94 (MQ=60)
                                                                                  ATGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAG          <  SRR4302533.4509015/94‑1 (MQ=60)
                                                                                   TGCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGA         <  SRR4302533.979665/94‑1 (MQ=60)
                                                                                    GCAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGAC        <  SRR4302533.3793948/94‑1 (MQ=60)
                                                                                     CAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACT       >  SRR4302533.1923076/1‑94 (MQ=60)
                                                                                     CAGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACT       <  SRR4302533.272386/94‑1 (MQ=60)
                                                                                      AGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTT      >  SRR4302533.4518706/1‑94 (MQ=60)
                                                                                      AGACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTT      <  SRR4302533.2368652/94‑1 (MQ=60)
                                                                                        ACAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTAC    >  SRR4302533.1534942/1‑94 (MQ=60)
                                                                                         CAACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAAAGACTTACA   >  SRR4302533.3190466/1‑94 (MQ=60)
                                                                                          AACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  >  SRR4302533.622602/1‑94 (MQ=60)
                                                                                          AACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  >  SRR4302533.4961509/1‑94 (MQ=60)
                                                                                          AACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  >  SRR4302533.2707911/1‑94 (MQ=60)
                                                                                          AACACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  <  SRR4302533.887068/94‑1 (MQ=60)
                                                                                           |                                                                                            
GCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCA  >  NC_000913/1734614‑1734797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑