Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,768,647 T→C A12A (GCT→GCC)  b1689 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,768,6470TC98.2% 883.9 / 2.5 279A12A (GCT→GCC) b1689hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (148/126);  total (150/129)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

Only 100 of 282 total aligned reads displayed.

ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT  >  NC_000913/1768558‑1768735
                                                                                         |                                                                                        
aTACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTAt                                                                                       <  1:4324794/93‑1 (MQ=255)
aTACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTAt                                                                                       <  2:9046225/93‑1 (MQ=255)
aTACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTAt                                                                                       <  2:8292721/93‑1 (MQ=255)
  aCTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGa                                                                                     <  2:3683732/93‑1 (MQ=255)
   cTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGaa                                                                                    <  1:10030010/93‑1 (MQ=255)
   cTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGaa                                                                                    <  2:6589784/93‑1 (MQ=255)
    tgtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAc                                                                                   >  1:8147431/1‑93 (MQ=255)
     gtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACt                                                                                  <  2:879238/93‑1 (MQ=255)
       gACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTcc                                                                                <  1:7659025/93‑1 (MQ=255)
        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                               >  1:1259474/1‑93 (MQ=255)
        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                               <  1:5675361/93‑1 (MQ=255)
        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                               <  2:7379691/93‑1 (MQ=255)
        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                               >  2:8273360/1‑93 (MQ=255)
        aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                               <  2:9768599/93‑1 (MQ=255)
         ctctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAg                                                                              <  2:2713488/93‑1 (MQ=255)
          tctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAgg                                                                             >  1:7908251/1‑93 (MQ=255)
            tctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCa                                                                           <  1:7716733/93‑1 (MQ=255)
             ctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAc                                                                          >  2:10014440/1‑93 (MQ=255)
             ctTCAAGCCGTAAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAc                                                                          >  2:6713998/1‑93 (MQ=255)
              ttCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACt                                                                         >  2:3751127/1‑93 (MQ=255)
               tCAAGCCGTCAGCAGTCTGATAATGTTATTTATATTTTATGGGTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTc                                                                        <  1:6260737/93‑1 (MQ=255)
               tCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTc                                                                        <  1:3351346/93‑1 (MQ=255)
                  aGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCCGGCACTCCGc                                                                     >  1:5324958/1‑93 (MQ=255)
                    ccGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTGCAGGCACTCCGCCa                                                                   <  1:5393612/93‑1 (MQ=255)
                      gTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCata                                                                 <  2:9903956/93‑1 (MQ=255)
                      gTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCata                                                                 <  2:5649480/93‑1 (MQ=255)
                         agcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttt                                                              <  1:3528576/93‑1 (MQ=255)
                         agcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCAAGGCACTCCGCCATAttt                                                              >  2:3291203/1‑93 (MQ=255)
                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                          >  1:6112558/1‑93 (MQ=255)
                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                          <  2:1050017/93‑1 (MQ=255)
                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                          >  1:9513742/1‑93 (MQ=255)
                             ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                          <  1:4399472/93‑1 (MQ=255)
                              gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGcc                                                         >  1:9179664/1‑93 (MQ=255)
                                 gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg                                                      >  1:2309608/1‑93 (MQ=255)
                                 gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg                                                      >  2:4316173/1‑93 (MQ=255)
                                  aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa                                                     <  2:2466358/93‑1 (MQ=255)
                                  aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa                                                     >  1:2062125/1‑93 (MQ=255)
                                   tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc                                                    >  2:3335770/1‑93 (MQ=255)
                                    aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACt                                                   >  1:4894839/1‑93 (MQ=255)
                                     aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa                                                  >  1:1920638/1‑93 (MQ=255)
                                      tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                 <  2:7662604/93‑1 (MQ=255)
                                      tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                 <  1:2943748/93‑1 (MQ=255)
                                      tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                 <  2:8884079/93‑1 (MQ=255)
                                       gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt                                                <  2:4730607/93‑1 (MQ=255)
                                       gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt                                                >  2:6688577/1‑93 (MQ=255)
                                        ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg                                               <  1:8612056/93‑1 (MQ=255)
                                        ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg                                               <  2:1902242/93‑1 (MQ=255)
                                         tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga                                              >  1:7199057/1‑93 (MQ=255)
                                         tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga                                              <  1:889896/93‑1 (MQ=255)
                                         tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga                                              >  1:2586538/1‑93 (MQ=255)
                                          atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat                                             >  2:2769750/1‑93 (MQ=255)
                                           tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg                                            <  2:5712913/93‑1 (MQ=255)
                                           tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg                                            >  2:9250192/1‑93 (MQ=255)
                                            ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatga                                           <  1:8259723/93‑1 (MQ=255)
                                             tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa                                          <  1:6953247/93‑1 (MQ=255)
                                             tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa                                          <  1:4588696/93‑1 (MQ=255)
                                              atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                         >  1:5148141/1‑93 (MQ=255)
                                              atatTTTATTGTTTATTCAATGCAGCCATTACACGATGAGCGCCTATGACCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                         <  2:9337574/93‑1 (MQ=255)
                                               tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg                                        <  1:8325115/93‑1 (MQ=255)
                                                 ttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg                                      >  2:4073734/1‑93 (MQ=255)
                                                  tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc                                     >  2:4994145/1‑93 (MQ=255)
                                                   ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg                                    >  1:1550737/1‑93 (MQ=255)
                                                    tATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGa                                   >  1:1655427/1‑93 (MQ=255)
                                                     aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                  >  1:3674079/1‑93 (MQ=255)
                                                     aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                  >  2:4303902/1‑93 (MQ=255)
                                                     aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                  <  2:1149242/93‑1 (MQ=255)
                                                       tGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt                                <  1:955585/93‑1 (MQ=255)
                                                       tGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt                                <  1:6378492/93‑1 (MQ=255)
                                                        gTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg                               <  2:10040152/93‑1 (MQ=255)
                                                          ttATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGa                             <  2:8672384/93‑1 (MQ=255)
                                                           tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGCCCTGGAt                            >  2:2199162/1‑93 (MQ=255)
                                                           tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt                            <  2:6271239/93‑1 (MQ=255)
                                                            aTTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt                           <  2:3629140/93‑1 (MQ=255)
                                                              ttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGc                         >  1:7345073/1‑93 (MQ=255)
                                                                cATGCAGCCATTATACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc                       >  2:2371286/1‑93 (MQ=255)
                                                                 aTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa                      >  1:589847/1‑93 (MQ=255)
                                                                 aTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa                      >  1:7223240/1‑93 (MQ=255)
                                                                 aTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa                      >  1:9307230/1‑93 (MQ=255)
                                                                  tGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg                     >  2:1664644/1‑93 (MQ=255)
                                                                   gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                    >  2:127291/1‑93 (MQ=255)
                                                                   gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                    <  2:8037613/93‑1 (MQ=255)
                                                                      gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                 >  2:6923774/1‑93 (MQ=255)
                                                                        cATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt               <  1:9034856/93‑1 (MQ=255)
                                                                        cATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt               >  1:6716685/1‑93 (MQ=255)
                                                                        cATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt               >  1:3964222/1‑93 (MQ=255)
                                                                         aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg              >  2:2624606/1‑93 (MQ=255)
                                                                         aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg              >  1:6790669/1‑93 (MQ=255)
                                                                         aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg              >  2:9448705/1‑93 (MQ=255)
                                                                          ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa             >  2:9565108/1‑93 (MQ=255)
                                                                          ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa             >  1:6641378/1‑93 (MQ=255)
                                                                           tACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc            <  1:324693/93‑1 (MQ=255)
                                                                           tACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc            <  2:10001030/93‑1 (MQ=255)
                                                                           tACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc            <  1:2792538/93‑1 (MQ=255)
                                                                             cacGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg          <  2:5769598/93‑1 (MQ=255)
                                                                              acGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt         >  1:4185485/1‑93 (MQ=255)
                                                                                gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa       >  1:2154956/1‑93 (MQ=255)
                                                                                gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa       >  2:6016708/1‑93 (MQ=255)
                                                                                  tGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa     >  2:2871852/1‑93 (MQ=255)
                                                                                    aaCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc   <  2:1521399/93‑1 (MQ=255)
                                                                                     aCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt  <  1:9814605/93‑1 (MQ=255)
                                                                                         |                                                                                        
ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT  >  NC_000913/1768558‑1768735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  NC_000913/1768555‑1768740
                                                                                            |                                                                                             
AAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCT                                                                                              >  SRR4302540.535177/1‑94 (MQ=60)
   ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           <  SRR4302540.4371882/94‑1 (MQ=60)
   ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           <  SRR4302540.4371883/94‑1 (MQ=60)
   ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           >  SRR4302540.7071380/1‑94 (MQ=60)
   ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           >  SRR4302540.7071379/1‑94 (MQ=60)
   ATACTGGGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           <  SRR4302540.8206147/94‑1 (MQ=60)
   ATACCGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           <  SRR4302540.1756073/94‑1 (MQ=60)
     ACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAA                                                                                         >  SRR4302540.6336601/1‑94 (MQ=60)
      CTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAC                                                                                        <  SRR4302540.10146691/94‑1 (MQ=60)
      CTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAC                                                                                        >  SRR4302540.6913392/1‑94 (MQ=60)
      CTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAC                                                                                        >  SRR4302540.8242009/1‑94 (MQ=60)
          GACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCA                                                                                    <  SRR4302540.7745267/94‑1 (MQ=60)
          GACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCA                                                                                    >  SRR4302540.1272394/1‑94 (MQ=60)
           ACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAG                                                                                   <  SRR4302540.3020127/94‑1 (MQ=60)
           ACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAG                                                                                   <  SRR4302540.5737853/94‑1 (MQ=60)
           ACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAG                                                                                   >  SRR4302540.6051951/1‑94 (MQ=60)
           ACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAG                                                                                   <  SRR4302540.7406622/94‑1 (MQ=60)
            CTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAcc                                                                                  >  SRR4302540.9877960/1‑92 (MQ=60)
            CTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGG                                                                                  <  SRR4302540.9326222/94‑1 (MQ=60)
            CTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGG                                                                                  >  SRR4302540.8000468/1‑94 (MQ=60)
              CTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCA                                                                                >  SRR4302540.5958104/1‑94 (MQ=60)
              CTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCA                                                                                >  SRR4302540.5433502/1‑94 (MQ=60)
              CTCCTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGTCATTACACGATGAACGCCTATGAACTCCAGccc                                                                                >  SRR4302540.8422890/1‑91 (MQ=60)
               TCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAC                                                                               <  SRR4302540.7805866/94‑1 (MQ=60)
               TCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAC                                                                               <  SRR4302540.7805865/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGTCTGATAATGTTATTTATATTTTATGGGTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.6330392/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.3388084/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.8364316/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.8364322/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.7582703/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.6330391/94‑1 (MQ=60)
                  TCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCC                                                                            <  SRR4302540.4992625/94‑1 (MQ=60)
                    AAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCCGGCACTCCGC                                                                          >  SRR4302540.5383285/1‑94 (MQ=60)
                     AGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCC                                                                         >  SRR4302540.607516/1‑94 (MQ=60)
                       CCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTGCAGGCACTCCGCCAT                                                                       <  SRR4302540.5452915/94‑1 (MQ=60)
                        CGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATA                                                                      <  SRR4302540.2420523/94‑1 (MQ=60)
                         GTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAT                                                                     >  SRR4302540.9478301/1‑94 (MQ=60)
                         GTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAT                                                                     >  SRR4302540.6752417/1‑94 (MQ=60)
                         GTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAT                                                                     <  SRR4302540.10083189/94‑1 (MQ=60)
                          TCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATT                                                                    >  SRR4302540.4983677/1‑94 (MQ=60)
                          TCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATT                                                                    <  SRR4302540.4187061/94‑1 (MQ=60)
                           CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   <  SRR4302540.4613646/94‑1 (MQ=60)
                           CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   <  SRR4302540.3316459/94‑1 (MQ=60)
                           CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   >  SRR4302540.4499677/1‑94 (MQ=60)
                            AGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTT                                                                  <  SRR4302540.3567065/94‑1 (MQ=60)
                             GCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTT                                                                 <  SRR4302540.7862935/94‑1 (MQ=60)
                              CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTG                                                                >  SRR4302540.3985273/1‑94 (MQ=60)
                               AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGC                                                               >  SRR4302540.9626429/1‑94 (MQ=60)
                               AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGC                                                               >  SRR4302540.6180626/1‑94 (MQ=60)
                                GGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCATACCGCCATATTTTTGCC                                                              >  SRR4302540.9097499/1‑94 (MQ=60)
                                GGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCC                                                              >  SRR4302540.9289763/1‑94 (MQ=60)
                                GGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCC                                                              <  SRR4302540.4447190/94‑1 (MQ=60)
                                GGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCC                                                              >  SRR4302540.9289762/1‑94 (MQ=60)
                                 GCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCA                                                             <  SRR4302540.1533939/94‑1 (MQ=60)
                                 GCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCA                                                             <  SRR4302540.1533940/94‑1 (MQ=60)
                                 GCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCA                                                             >  SRR4302540.9664459/1‑94 (MQ=60)
                                   TGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATG                                                           >  SRR4302540.2334473/1‑94 (MQ=60)
                                   TGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATG                                                           >  SRR4302540.4855851/1‑94 (MQ=60)
                                    GATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGA                                                          <  SRR4302540.3695629/94‑1 (MQ=60)
                                    GATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGA                                                          >  SRR4302540.2084315/1‑94 (MQ=60)
                                      TAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACT                                                        >  SRR4302540.1657702/1‑94 (MQ=60)
                                      TAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACT                                                        >  SRR4302540.4947479/1‑94 (MQ=60)
                                       AATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTA                                                       >  SRR4302540.1941590/1‑94 (MQ=60)
                                       AATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTA                                                       >  SRR4302540.6712037/1‑94 (MQ=60)
                                        ATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAT                                                      >  SRR4302540.2924738/1‑94 (MQ=60)
                                         TGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATT                                                     <  SRR4302540.2976111/94‑1 (MQ=60)
                                         TGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATT                                                     <  SRR4302540.9387111/94‑1 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   <  SRR4302540.8712125/94‑1 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   <  SRR4302540.2458850/94‑1 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302540.2614418/1‑94 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   <  SRR4302540.9004182/94‑1 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302540.4051127/1‑94 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302540.7279312/1‑94 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302540.7510989/1‑94 (MQ=60)
                                           TTATTTATATTTTATTGTTTATTGCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   <  SRR4302540.6233853/94‑1 (MQ=60)
                                            TATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGAT                                                  >  SRR4302540.5902333/1‑94 (MQ=60)
                                            TATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGAT                                                  <  SRR4302540.899352/94‑1 (MQ=60)
                                             TTTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATG                                                 <  SRR4302540.2079408/94‑1 (MQ=60)
                                             ATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATG                                                 >  SRR4302540.2241546/1‑94 (MQ=60)
                                              TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302540.5500626/1‑94 (MQ=60)
                                              TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302540.9448870/1‑94 (MQ=60)
                                              TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302540.1663744/1‑94 (MQ=60)
                                              TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302540.3937848/1‑94 (MQ=60)
                                              TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302540.7772195/1‑94 (MQ=60)
                                               TTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAA                                               <  SRR4302540.8355706/94‑1 (MQ=60)
                                               TTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAA                                               >  SRR4302540.8489635/1‑94 (MQ=60)
                                                TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              <  SRR4302540.7030606/94‑1 (MQ=60)
                                                TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              <  SRR4302540.4638103/94‑1 (MQ=60)
                                                TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              >  SRR4302540.4805138/1‑94 (MQ=60)
                                                TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              >  SRR4302540.5203929/1‑94 (MQ=60)
                                                 ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             >  SRR4302540.8268115/1‑94 (MQ=60)
                                                 ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             >  SRR4302540.7287952/1‑94 (MQ=60)
                                                  TATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGC                                            <  SRR4302540.5303300/94‑1 (MQ=60)
                                                  TATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGC                                            <  SRR4302540.8421737/94‑1 (MQ=60)
                                                  TATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGC                                            <  SRR4302540.4324678/94‑1 (MQ=60)
                                                  TATTTTATTGTTTATTCCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGC                                            <  SRR4302540.686851/94‑1 (MQ=60)
                                                     TTTATTTTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCG                                         <  SRR4302540.8667238/94‑1 (MQ=60)
                                                     TTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCG                                         >  SRR4302540.1567826/1‑94 (MQ=60)
                                                     TTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCG                                         >  SRR4302540.3219292/1‑94 (MQ=60)
                                                      TTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGA                                        >  SRR4302540.1673468/1‑94 (MQ=60)
                                                      TTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGA                                        >  SRR4302540.1265899/1‑94 (MQ=60)
                                                       TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       <  SRR4302540.2760583/94‑1 (MQ=60)
                                                       TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       >  SRR4302540.3714254/1‑94 (MQ=60)
                                                          TGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTG                                    <  SRR4302540.6449503/94‑1 (MQ=60)
                                                          TGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTG                                    <  SRR4302540.965491/94‑1 (MQ=60)
                                                           GTTTATTTCATGCAGCCATTACAGGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGG                                   <  SRR4302540.2952433/94‑1 (MQ=60)
                                                            TTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGA                                  >  SRR4302540.9663249/1‑94 (MQ=60)
                                                             TTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAT                                 >  SRR4302540.370670/1‑94 (MQ=60)
                                                              TATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATT                                >  SRR4302540.8322545/1‑94 (MQ=60)
                                                              TATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATT                                >  SRR4302540.7347323/1‑94 (MQ=60)
                                                                TTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGC                              >  SRR4302540.7427025/1‑94 (MQ=60)
                                                                  TCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCC                            >  SRR4302540.6514602/1‑94 (MQ=60)
                                                                   CATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCA                           >  SRR4302540.7303760/1‑94 (MQ=60)
                                                                   CATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCA                           >  SRR4302540.596204/1‑94 (MQ=60)
                                                                   CATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCA                           >  SRR4302540.9757115/1‑94 (MQ=60)
                                                                   CATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCA                           >  SRR4302540.9418366/1‑94 (MQ=60)
                                                                    ATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAG                          >  SRR4302540.10052079/1‑94 (MQ=60)
                                                                    ATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAG                          >  SRR4302540.5551260/1‑94 (MQ=60)
                                                                      GCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAC                        >  SRR4302540.2802010/1‑94 (MQ=60)
                                                                      GCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAC                        <  SRR4302540.3426615/94‑1 (MQ=60)
                                                                       CAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACC                       >  SRR4302540.5353131/1‑94 (MQ=60)
                                                                         GCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGG                     <  SRR4302540.3395263/94‑1 (MQ=60)
                                                                          CCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGT                    >  SRR4302540.6790785/1‑94 (MQ=60)
                                                                          CCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGT                    >  SRR4302540.4006966/1‑94 (MQ=60)
                                                                           CATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTG                   >  SRR4302540.6865770/1‑94 (MQ=60)
                                                                           CATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTG                   >  SRR4302540.215104/1‑94 (MQ=60)
                                                                           CATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTG                   <  SRR4302540.9143284/94‑1 (MQ=60)
                                                                            ATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGA                  >  SRR4302540.6714865/1‑94 (MQ=60)
                                                                            ATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGA                  >  SRR4302540.7896782/1‑94 (MQ=60)
                                                                             TTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAC                 <  SRR4302540.675077/94‑1 (MQ=60)
                                                                              TACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACA                <  SRR4302540.2822685/94‑1 (MQ=60)
                                                                              TACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACA                <  SRR4302540.327944/94‑1 (MQ=60)
                                                                              TACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACA                <  SRR4302540.8805879/94‑1 (MQ=60)
                                                                              TACACGATGAACGCCTATGAACTCCAGGAACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACA                <  SRR4302540.8805612/94‑1 (MQ=60)
                                                                               ACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAG               >  SRR4302540.4378421/1‑94 (MQ=60)
                                                                                CACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGT              >  SRR4302540.4230973/1‑94 (MQ=60)
                                                                                  CGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGA            >  SRR4302540.2178147/1‑94 (MQ=60)
                                                                                      GAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC        >  SRR4302540.2071097/1‑94 (MQ=60)
                                                                                      GAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC        >  SRR4302540.1931685/1‑94 (MQ=60)
                                                                                       AACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT       >  SRR4302540.6830100/1‑94 (MQ=60)
                                                                                       AACGCTTATGAACTCCAGGCACCCCGCCATATTTTTGCCATGACTATTGATGAATGAGCGACCTGGATTGCCCAGACCGGTGGCAGTGAACGCT       >  SRR4302540.6830101/1‑94 (MQ=60)
                                                                                       AACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGACTGCCCAGACCGGTGACAGTGAAAGCT       >  SRR4302540.2184294/1‑94 (MQ=60)
                                                                                        ACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTG      <  SRR4302540.9929458/94‑1 (MQ=60)
                                                                                          GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    >  SRR4302540.2072766/1‑94 (MQ=60)
                                                                                          GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    <  SRR4302540.10036909/94‑1 (MQ=60)
                                                                                          GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    >  SRR4302540.8020114/1‑94 (MQ=60)
                                                                                          GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    <  SRR4302540.5154740/94‑1 (MQ=60)
                                                                                          GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    >  SRR4302540.8019819/1‑94 (MQ=60)
                                                                                           CCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCG   >  SRR4302540.927107/1‑94 (MQ=60)
                                                                                           CCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCG   <  SRR4302540.4361691/94‑1 (MQ=60)
                                                                                            CTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGATCGGTGACAGTGAAAGCTAGCGT  >  SRR4302540.3487325/1‑94 (MQ=60)
                                                                                            CTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  SRR4302540.4029801/1‑94 (MQ=60)
                                                                                            |                                                                                             
AAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  NC_000913/1768555‑1768740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑