Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,776,191 T→C R325R (CGT→CGC)  b1695 → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,776,1910TC99.1% 675.6 / ‑2.8 215R325R (CGT→CGC) b1695putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (114/99);  total (116/99)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 218 total aligned reads displayed.

CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776102‑1776280
                                                                                         |                                                                                         
cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        >  2:8680624/1‑93 (MQ=255)
 gcAACATGGGGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       <  1:2195778/93‑1 (MQ=255)
   aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:9679487/1‑93 (MQ=255)
     cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAt                                                                                   <  1:9133031/93‑1 (MQ=255)
     cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCCCAAt                                                                                   >  2:3120084/1‑93 (MQ=255)
      aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  2:9041008/93‑1 (MQ=255)
       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:9594902/93‑1 (MQ=255)
       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:7857586/93‑1 (MQ=255)
       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  1:3464525/93‑1 (MQ=255)
        ggTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGa                                                                                <  2:1770652/93‑1 (MQ=255)
         gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  1:7162178/93‑1 (MQ=255)
           gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             <  1:1115156/93‑1 (MQ=255)
             tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  1:8351654/93‑1 (MQ=255)
             tGAAAGGGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  2:2150254/93‑1 (MQ=255)
               aaaGTGCCACGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:1959721/93‑1 (MQ=255)
                aaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtg                                                                        >  2:4183547/1‑93 (MQ=255)
                 aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:9351584/93‑1 (MQ=255)
                   gggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatg                                                                     <  1:8501578/92‑1 (MQ=255)
                    ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:1575780/93‑1 (MQ=255)
                    ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    >  2:6251519/1‑93 (MQ=255)
                    ggcacggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  2:8147655/93‑1 (MQ=255)
                     gcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  1:9021944/93‑1 (MQ=255)
                     gcatgacaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  2:5652850/93‑1 (MQ=255)
                      catggcaAGCGGATCAGAATCAGTCGCTGCGCCCCAGCGGGGCGCTGGCAAAACTGTCCTGTGCACGCACCGCAATGGAAGTCATTGatgatg                                                                  <  2:2643183/93‑1 (MQ=255)
                       atgccaACCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 <  2:8452783/93‑1 (MQ=255)
                        tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:7981057/1‑93 (MQ=255)
                        tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:7920649/1‑93 (MQ=255)
                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:6024850/93‑1 (MQ=255)
                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  2:7291045/1‑93 (MQ=255)
                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  2:6061564/1‑93 (MQ=255)
                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:482188/93‑1 (MQ=255)
                          gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              >  2:3879283/1‑93 (MQ=255)
                            aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  1:3868768/1‑93 (MQ=255)
                               ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         >  2:4974963/1‑93 (MQ=255)
                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  1:5500011/1‑93 (MQ=255)
                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  1:1056978/1‑93 (MQ=255)
                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       <  1:8042727/93‑1 (MQ=255)
                                  aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACCGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGTTTCAAATCa                                                      >  2:6960421/1‑93 (MQ=255)
                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:3847055/1‑93 (MQ=255)
                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:570866/1‑93 (MQ=255)
                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:1262804/93‑1 (MQ=255)
                                   cgaGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:5058750/91‑1 (MQ=255)
                                    cAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATg                                                    <  1:6218937/93‑1 (MQ=255)
                                     aGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATgg                                                   <  2:4870334/93‑1 (MQ=255)
                                      gCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATggg                                                  <  1:2121465/93‑1 (MQ=255)
                                      gCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATggg                                                  <  1:2350534/93‑1 (MQ=255)
                                       cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 >  2:1257539/1‑93 (MQ=255)
                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:6361565/1‑93 (MQ=255)
                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:9222286/1‑93 (MQ=255)
                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:2256722/1‑93 (MQ=255)
                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  1:8190384/1‑93 (MQ=255)
                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:8341353/1‑93 (MQ=255)
                                            gTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCt                                            >  2:2727736/1‑93 (MQ=255)
                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:3682947/1‑93 (MQ=255)
                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:2286465/1‑93 (MQ=255)
                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:6701875/1‑93 (MQ=255)
                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       <  1:7486961/93‑1 (MQ=255)
                                                  gcgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      >  2:4722592/1‑93 (MQ=255)
                                                   cgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtat                                     <  2:3923582/93‑1 (MQ=255)
                                                     cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   <  1:324245/93‑1 (MQ=255)
                                                     cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   <  2:2120475/93‑1 (MQ=255)
                                                        cAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGa                                >  2:1677121/1‑93 (MQ=255)
                                                          gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  2:8866367/93‑1 (MQ=255)
                                                          gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  1:5909248/93‑1 (MQ=255)
                                                            gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            <  1:235627/93‑1 (MQ=255)
                                                            gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            >  2:7547295/1‑93 (MQ=255)
                                                             cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCCGCGCTATACAGATGAgg                           >  2:9780547/1‑93 (MQ=255)
                                                              ggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgc                          >  2:7093182/1‑93 (MQ=255)
                                                               gcgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcg                         >  2:3487865/1‑93 (MQ=255)
                                                               gcgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcg                         <  1:1936907/93‑1 (MQ=255)
                                                                cgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgc                        >  2:7587910/1‑93 (MQ=255)
                                                                cgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgc                        <  2:6579062/93‑1 (MQ=255)
                                                                 gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  1:2081934/93‑1 (MQ=255)
                                                                 gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  1:9888957/93‑1 (MQ=255)
                                                                  cTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc                      >  1:7325954/1‑93 (MQ=255)
                                                                   tGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGcgcgcg                     >  1:296975/1‑93 (MQ=255)
                                                                    ggCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGt                    <  2:1985357/93‑1 (MQ=255)
                                                                    ggCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGt                    <  1:8873637/93‑1 (MQ=255)
                                                                     gCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtc                   >  1:864698/1‑93 (MQ=255)
                                                                      cAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtct                  <  2:4001161/93‑1 (MQ=255)
                                                                       aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 <  1:7394901/93‑1 (MQ=255)
                                                                       aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 >  1:1752366/1‑93 (MQ=255)
                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                <  1:3672234/93‑1 (MQ=255)
                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                >  1:6856521/1‑93 (MQ=255)
                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGACATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGAGCGTCTcc                >  1:4401112/1‑93 (MQ=255)
                                                                         aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               <  2:5282316/93‑1 (MQ=255)
                                                                         aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               >  1:6606368/1‑93 (MQ=255)
                                                                          actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:9404640/93‑1 (MQ=255)
                                                                          actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:1094348/93‑1 (MQ=255)
                                                                            tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  1:4131953/1‑93 (MQ=255)
                                                                            tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  2:2481384/1‑93 (MQ=255)
                                                                              tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  2:1432151/1‑93 (MQ=255)
                                                                                 tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       <  1:3982579/93‑1 (MQ=255)
                                                                                 tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       >  2:1513447/1‑93 (MQ=255)
                                                                                  gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  2:5403996/1‑93 (MQ=255)
                                                                                  gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  2:9115146/1‑93 (MQ=255)
                                                                                   tgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg     >  1:5265743/1‑93 (MQ=255)
                                                                                   tgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg     >  2:7082291/1‑93 (MQ=255)
                                                                                     cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:5667757/1‑93 (MQ=255)
                                                                                      aCGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGACGAGGCGCGCGTCTCCCGCTTCTGGCGTGa  >  2:3255825/1‑93 (MQ=255)
                                                                                         |                                                                                         
CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776102‑1776280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  NC_000913/1776099‑1776284
                                                                                            |                                                                                             
ATGCGCAACATGGTGCTGAAAGTGGCATGGCCAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCA                                                                                              >  SRR4302540.5155939/1‑94 (MQ=60)
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCA                                                                                              >  SRR4302540.7794805/1‑94 (MQ=60)
   CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCG                                                                                           <  SRR4302540.1835853/94‑1 (MQ=60)
    GCAACATGGGGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGC                                                                                          <  SRR4302540.2219494/94‑1 (MQ=60)
     CAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCA                                                                                         >  SRR4302540.9793374/1‑94 (MQ=60)
       ACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAT                                                                                       <  SRR4302540.4469242/94‑1 (MQ=60)
        CATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATG                                                                                      <  SRR4302540.9795582/94‑1 (MQ=60)
        CATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATG                                                                                      <  SRR4302540.9242797/94‑1 (MQ=60)
          TGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGA                                                                                    <  SRR4302540.3502235/94‑1 (MQ=60)
            GTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAG                                                                                  <  SRR4302540.7242023/94‑1 (MQ=60)
            GTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAG                                                                                  <  SRR4302540.3397516/94‑1 (MQ=60)
              GCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTC                                                                                <  SRR4302540.1126708/94‑1 (MQ=60)
              GCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTC                                                                                <  SRR4302540.9368664/94‑1 (MQ=60)
                TGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAT                                                                              <  SRR4302540.8448582/94‑1 (MQ=60)
                 gnnannngnntgcCAAGNCGNNNAGNATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATT                                                                             <  SRR4302540.9311/81‑1 (MQ=60)
                  AAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTG                                                                            >  SRR4302540.7167423/1‑94 (MQ=60)
                  AAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTG                                                                            <  SRR4302540.2191169/94‑1 (MQ=60)
                    AGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGAT                                                                          >  SRR4302540.4139490/1‑94 (MQ=60)
                      GGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGA                                                                        <  SRR4302540.8600367/94‑1 (MQ=60)
                       GGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGAT                                                                       <  SRR4302540.1593024/94‑1 (MQ=60)
                        GCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATG                                                                      <  SRR4302540.9130203/94‑1 (MQ=60)
                         CATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGC                                                                     >  SRR4302540.3943578/1‑94 (MQ=60)
                          ATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCG                                                                    >  SRR4302540.5547942/1‑94 (MQ=60)
                          ATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCG                                                                    >  SRR4302540.1665379/1‑94 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  <  SRR4302540.6091703/94‑1 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  <  SRR4302540.487352/94‑1 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  <  SRR4302540.8586128/94‑1 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCCCACCAGCGCGGCGCTGGCAAAACTCTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  >  SRR4302540.5444904/1‑94 (MQ=60)
                              CAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTC                                                                >  SRR4302540.3910824/1‑94 (MQ=60)
                              CAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTC                                                                >  SRR4302540.3910823/1‑94 (MQ=60)
                               AAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCA                                                               <  SRR4302540.6730814/94‑1 (MQ=60)
                                   CGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATC                                                           >  SRR4302540.5560573/1‑94 (MQ=60)
                                   CGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATC                                                           >  SRR4302540.1067736/1‑94 (MQ=60)
                                   CGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATC                                                           >  SRR4302540.1067737/1‑94 (MQ=60)
                                    GATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCA                                                          <  SRR4302540.8136177/94‑1 (MQ=60)
                                      cgAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATG                                                        <  SRR4302540.5113446/92‑1 (MQ=60)
                                      TCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATG                                                        <  SRR4302540.1275769/94‑1 (MQ=60)
                                       CAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGG                                                       <  SRR4302540.6288124/94‑1 (MQ=60)
                                       CAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGG                                                       <  SRR4302540.8081520/94‑1 (MQ=60)
                                        AGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGG                                                      >  SRR4302540.5703873/1‑94 (MQ=60)
                                        AGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGG                                                      >  SRR4302540.9661791/1‑94 (MQ=60)
                                        AGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGG                                                      >  SRR4302540.1107715/1‑94 (MQ=60)
                                         GCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGC                                                     <  SRR4302540.2144226/94‑1 (MQ=60)
                                         GCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGC                                                     <  SRR4302540.2375844/94‑1 (MQ=60)
                                           ATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGG                                                   >  SRR4302540.8312060/1‑94 (MQ=60)
                                           ATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGG                                                   >  SRR4302540.8285555/1‑94 (MQ=60)
                                           ATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGG                                                   >  SRR4302540.4875144/1‑94 (MQ=60)
                                               GTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTG                                               >  SRR4302540.8845186/1‑94 (MQ=60)
                                                 CACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGG                                             <  SRR4302540.3988506/94‑1 (MQ=60)
                                                  CCTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGC                                            <  SRR4302540.1519268/94‑1 (MQ=60)
                                                  ACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGC                                            >  SRR4302540.5183733/1‑94 (MQ=60)
                                                    TGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTA                                          >  SRR4302540.2006633/1‑94 (MQ=60)
                                                    TGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTA                                          <  SRR4302540.7570537/94‑1 (MQ=60)
                                                     GCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTAT                                         <  SRR4302540.3256440/94‑1 (MQ=60)
                                                      CGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATA                                        >  SRR4302540.5852916/1‑94 (MQ=60)
                                                       GCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAC                                       <  SRR4302540.7712735/94‑1 (MQ=60)
                                                        CACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACC                                      <  SRR4302540.327494/94‑1 (MQ=60)
                                                             GCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGA                                 <  SRR4302540.5974535/94‑1 (MQ=60)
                                                              CGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAG                                <  SRR4302540.8169155/94‑1 (MQ=60)
                                                               GCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGG                               <  SRR4302540.237910/94‑1 (MQ=60)
                                                               GCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGG                               <  SRR4302540.237909/94‑1 (MQ=60)
                                                               GCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGG                               <  SRR4302540.6096590/94‑1 (MQ=60)
                                                                  GCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGC                            <  SRR4302540.1958009/94‑1 (MQ=60)
                                                                   CGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCG                           >  SRR4302540.8781859/1‑94 (MQ=60)
                                                                    GCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGC                          <  SRR4302540.2104302/94‑1 (MQ=60)
                                                                    GCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGC                          <  SRR4302540.10004422/94‑1 (MQ=60)
                                                                    GCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGC                          >  SRR4302540.7407752/1‑94 (MQ=60)
                                                                     CTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCG                         >  SRR4302540.299915/1‑94 (MQ=60)
                                                                     CTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCG                         >  SRR4302540.1151994/1‑94 (MQ=60)
                                                                      TGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGT                        >  SRR4302540.4264634/1‑94 (MQ=60)
                                                                       GGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTC                       >  SRR4302540.7375636/1‑94 (MQ=60)
                                                                       GGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTC                       <  SRR4302540.8976333/94‑1 (MQ=60)
                                                                       GGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTC                       >  SRR4302540.873881/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     >  SRR4302540.7490148/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     >  SRR4302540.5558539/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     >  SRR4302540.1771350/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     >  SRR4302540.1771349/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     >  SRR4302540.2381849/1‑94 (MQ=60)
                                                                         CAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTC                     <  SRR4302540.6599614/94‑1 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCC                    >  SRR4302540.7417648/1‑94 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCC                    <  SRR4302540.7477296/94‑1 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCC                    >  SRR4302540.6932491/1‑94 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCC                    >  SRR4302540.2105530/1‑94 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGACATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGAGCGTCTCC                    >  SRR4302540.4448842/1‑94 (MQ=60)
                                                                           AAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCC                   <  SRR4302540.6059398/94‑1 (MQ=60)
                                                                           AAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCC                   >  SRR4302540.6679521/1‑94 (MQ=60)
                                                                           AAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCC                   <  SRR4302540.3712396/94‑1 (MQ=60)
                                                                            AACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCG                  <  SRR4302540.2669535/94‑1 (MQ=60)
                                                                            AACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCG                  <  SRR4302540.6322919/94‑1 (MQ=60)
                                                                             ACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGC                 <  SRR4302540.9516502/94‑1 (MQ=60)
                                                                             ACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGC                 <  SRR4302540.1105601/94‑1 (MQ=60)
                                                                              CTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCT                >  SRR4302540.4176644/1‑94 (MQ=60)
                                                                                  ACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTG            <  SRR4302540.9131721/94‑1 (MQ=60)
                                                                                   CTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGG           >  SRR4302540.2226486/1‑94 (MQ=60)
                                                                                    TGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGC          <  SRR4302540.4025468/94‑1 (MQ=60)
                                                                                    TGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGC          <  SRR4302540.8976382/94‑1 (MQ=60)
                                                                                    TGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGC          <  SRR4302540.8743996/94‑1 (MQ=60)
                                                                                     GTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCG         >  SRR4302540.5323208/1‑94 (MQ=60)
                                                                                       GCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTG       >  SRR4302540.5730167/1‑94 (MQ=60)
                                                                                         ACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGAT     <  SRR4302540.3619139/94‑1 (MQ=60)
                                                                                           GCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGT   <  SRR4302540.9333491/94‑1 (MQ=60)
                                                                                            CACCGCAATGGAAGTCATTGATGATGCGATTCAAATCCTGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGCTGTC  >  SRR4302540.7920773/1‑94 (MQ=60)
                                                                                            CACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  <  SRR4302540.8438170/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  NC_000913/1776099‑1776284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑