Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,795	T→C	S60G (AGT→GGT)	yeaR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,795	0	T	C	99.6%	804.8 / NA	250	S60G (AGT→GGT)	yeaR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (123/125); minor base G (0/1); total (124/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

cagtcaTTGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         <  1:31099/93‑1 (MQ=255)

cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         <  1:31097/93‑1 (MQ=255)

cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         >  2:2808733/1‑93 (MQ=255)

 agtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgtg                                                                                        >  1:5058838/1‑93 (MQ=255)

  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                       >  2:9838146/1‑93 (MQ=255)

  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                       <  1:4469788/93‑1 (MQ=255)

   tcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGtt                                                                                      >  1:7994393/1‑93 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     >  1:7244201/1‑93 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     <  1:7534647/93‑1 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     <  1:10012711/93‑1 (MQ=255)

     aTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGttaa                                                                                    >  1:2526390/1‑91 (MQ=255)

      tGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcat                                                                                   >  1:9307441/1‑93 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:7910997/93‑1 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:6154088/93‑1 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:3942920/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCTCCTGATCAGGCTCTGCACCGTGTtcatca                                                                                 <  1:3385212/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                 >  2:1073394/1‑93 (MQ=255)

          ttCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGc                                                                               >  1:6289682/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  2:5594564/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  1:2725954/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  1:2725957/1‑93 (MQ=255)

            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                             <  1:3719605/93‑1 (MQ=255)

            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                             >  2:2965623/1‑93 (MQ=255)

             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                            >  1:8398900/1‑93 (MQ=255)

               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                          >  1:2483580/1‑93 (MQ=255)

                 ttgtGCCACTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                        <  2:3440774/93‑1 (MQ=255)

                     gCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGat                                                                    >  1:662408/1‑93 (MQ=255)

                      ccACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGata                                                                   >  2:6358404/1‑93 (MQ=255)

                      ccACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGata                                                                   <  2:7961361/93‑1 (MQ=255)

                       cACTTTTCTGGAGGGATCACCGCAAACTGCCCCGCTTCGATAAGGATCAGCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGatat                                                                  <  2:5223327/93‑1 (MQ=255)

                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                <  1:9141692/93‑1 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                               >  2:1199148/1‑93 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGCATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                               >  1:415475/1‑93 (MQ=255)

                           tttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGa                                                              <  2:1539027/93‑1 (MQ=255)

                           tttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGa                                                              <  1:2965623/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:3823636/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  1:6396817/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:2725957/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:2725954/93‑1 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                          >  1:5280772/1‑93 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                          >  2:6709842/1‑93 (MQ=255)

                                 gAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGccc                                                        >  1:7671841/1‑93 (MQ=255)

                                 gAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATA‑TTGACCGcccc                                                       >  1:8764429/1‑93 (MQ=255)

                                  aGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcccc                                                       <  2:7472402/93‑1 (MQ=255)

                                   gggAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCa                                                      <  2:767754/93‑1 (MQ=255)

                                    ggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCAt                                                     <  2:2076972/93‑1 (MQ=255)

                                     gAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATg                                                    >  1:8100678/1‑93 (MQ=255)

                                      aaCACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGc                                                   <  2:5819476/93‑1 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                  >  1:4812899/1‑93 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               >  2:8858881/1‑93 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               <  2:3278365/93‑1 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               >  2:4332816/1‑93 (MQ=255)

                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                              <  1:5314333/93‑1 (MQ=255)

                                            gCAAACTGCCCCGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACg                                             <  1:9785531/93‑1 (MQ=255)

                                             cAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACgg                                            <  2:7388699/93‑1 (MQ=255)

                                              aaaCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGa                                           <  1:4409553/93‑1 (MQ=255)

                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaaa                                         >  2:6472312/1‑93 (MQ=255)

                                                 cgtccccGCTTCGATAAGGAGCACCTGATCAGTCTCTGCACCGTGGTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  1:9888385/90‑1 (MQ=255)

                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  2:9259796/93‑1 (MQ=255)

                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  2:9332530/93‑1 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                      >  2:5169108/1‑93 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                      <  1:1823622/93‑1 (MQ=255)

                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                     <  2:7330746/93‑1 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                  <  1:8476504/93‑1 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                  <  1:4340414/93‑1 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCGTAACGGAAAGGCGTggg                                 <  1:6886325/93‑1 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                 <  2:2548643/93‑1 (MQ=255)

                                                         cTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGt                                >  2:8953278/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               >  1:6234476/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               <  2:1738087/93‑1 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               >  2:4481906/1‑93 (MQ=255)

                                                            cGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTaaa                             <  2:6265143/93‑1 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCTGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  1:6550138/1‑93 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           <  2:2238534/93‑1 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  2:5504819/1‑93 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  2:9663101/1‑93 (MQ=255)

                                                                aaGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcccc                         <  1:9443839/93‑1 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAccccc                        <  1:2184542/93‑1 (MQ=255)

                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCgg                      <  1:1065921/93‑1 (MQ=255)

                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCgg                      <  1:8150519/93‑1 (MQ=255)

                                                                    aTCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCggg                     <  2:482365/93‑1 (MQ=255)

                                                                     tCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGgc                    >  2:8446264/1‑93 (MQ=255)

                                                                        ccTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGcgcg                 >  1:5980276/1‑93 (MQ=255)

                                                                          tGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGtt               <  1:52421/93‑1 (MQ=255)

                                                                           gATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTc              >  1:5943039/1‑93 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             >  2:1523970/1‑93 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             <  1:4618448/93‑1 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTGCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             <  2:7833575/93‑1 (MQ=255)

                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCt            >  1:145984/1‑93 (MQ=255)

                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCt            >  1:1501055/1‑93 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCtt           >  2:6735665/1‑93 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCACGGGCGCGCTCCtt           >  2:4275623/1‑93 (MQ=255)

                                                                               cggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCttt          <  2:8386767/92‑1 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTa         <  1:745224/93‑1 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTa         <  2:6593568/93‑1 (MQ=255)

                                                                                     tGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAg    >  2:3549013/1‑93 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   <  2:7188783/93‑1 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   <  1:3648331/93‑1 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   >  2:10035857/1‑93 (MQ=255)

                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAt  <  2:937958/93‑1 (MQ=255)

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACG  >  NC_000913/1877702‑1877888

CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACC                                                                                               <  SRR4302540.6270479/94‑1 (MQ=60)

CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACC                                                                                               >  SRR4302540.6914028/1‑94 (MQ=60)

 GTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCG                                                                                              >  SRR4302540.1413578/1‑94 (MQ=60)

   CAGTCATTGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302540.31425/94‑1 (MQ=60)

   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302540.31423/94‑1 (MQ=60)

   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            >  SRR4302540.5113536/1‑94 (MQ=60)

   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            >  SRR4302540.944475/1‑94 (MQ=60)

   CAGTCATGGCGTCCATGTTGTGCCACTGTTCTGAAGGGAACACCGCAAACTGCCCCGCTGCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302540.31424/94‑1 (MQ=60)

    AGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGT                                                                                           >  SRR4302540.430154/1‑94 (MQ=60)

     GTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTT                                                                                          >  SRR4302540.8087456/1‑94 (MQ=60)

     GTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTT                                                                                          <  SRR4302540.4518255/94‑1 (MQ=60)

      TCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTC                                                                                         >  SRR4302540.7325018/1‑94 (MQ=60)

      TCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTC                                                                                         >  SRR4302540.7612493/1‑94 (MQ=60)

      TCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTC                                                                                         >  SRR4302540.3441425/1‑94 (MQ=60)

       CATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCA                                                                                        <  SRR4302540.7619034/94‑1 (MQ=60)

       CATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCA                                                                                        <  SRR4302540.10129291/94‑1 (MQ=60)

       CATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTAA                                                                                        >  SRR4302540.2553657/1‑94 (MQ=60)

        ATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCAT                                                                                       >  SRR4302540.9418577/1‑94 (MQ=60)

         TGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATC                                                                                      >  SRR4302540.6034449/1‑94 (MQ=60)

         TGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATC                                                                                      <  SRR4302540.64425/94‑1 (MQ=60)

          GGCTTCAATGTTGTGCCTCTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     <  SRR4302540.3103859/94‑1 (MQ=60)

          GGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     <  SRR4302540.3985508/94‑1 (MQ=60)

          GGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     >  SRR4302540.5126784/1‑94 (MQ=60)

          GGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     <  SRR4302540.6222642/94‑1 (MQ=60)

          GGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     <  SRR4302540.8003238/94‑1 (MQ=60)

           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCTCCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302540.3422157/94‑1 (MQ=60)

           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302540.1613306/94‑1 (MQ=60)

            CTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGC                                                                                   >  SRR4302540.6359695/1‑94 (MQ=60)

            CTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGC                                                                                   <  SRR4302540.6790610/94‑1 (MQ=60)

             TTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATCAGGATCAACTGATCAGGCTCTGCACCGTGTTCATCAGCG                                                                                  >  SRR4302540.2755358/1‑94 (MQ=60)

             TTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCG                                                                                  <  SRR4302540.4865158/94‑1 (MQ=60)

             TTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCG                                                                                  >  SRR4302540.2755361/1‑94 (MQ=60)

             TTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCG                                                                                  >  SRR4302540.2755360/1‑94 (MQ=60)

              TCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGT                                                                                 <  SRR4302540.909220/94‑1 (MQ=60)

              TCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGT                                                                                 >  SRR4302540.8156556/1‑94 (MQ=60)

               CAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTA                                                                                >  SRR4302540.8496367/1‑94 (MQ=60)

               CAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTA                                                                                <  SRR4302540.3760190/94‑1 (MQ=60)

               CAATGTTGTGCCACTTCTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTA                                                                                <  SRR4302540.4602191/94‑1 (MQ=60)

                AATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAG                                                                               <  SRR4302540.8463274/94‑1 (MQ=60)

                 ATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGC                                                                              >  SRR4302540.2510340/1‑94 (MQ=60)

                 ATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGC                                                                              >  SRR4302540.4435976/1‑94 (MQ=60)

                   GTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCG                                                                            >  SRR4302540.6660995/1‑94 (MQ=60)

                     TGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAG                                                                          <  SRR4302540.3158622/94‑1 (MQ=60)

                       TGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGAT                                                                        >  SRR4302540.669507/1‑94 (MQ=60)

                        GCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATA                                                                       <  SRR4302540.1960285/94‑1 (MQ=60)

                            gttttcggggGGGAACACCGCAACCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   <  SRR4302540.6581109/84‑1 (MQ=60)

                            CTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   >  SRR4302540.7699751/1‑94 (MQ=60)

                            CTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   <  SRR4302540.9251516/94‑1 (MQ=60)

                            CTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGCATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   >  SRR4302540.419827/1‑94 (MQ=60)

                              TTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAC                                                                 <  SRR4302540.2998349/94‑1 (MQ=60)

                               TTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACC                                                                <  SRR4302540.4718646/94‑1 (MQ=60)

                               TTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACC                                                                <  SRR4302540.6468030/94‑1 (MQ=60)

                               TTCTGGAGGGAACACAGCAGACTGCCCCACTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACC                                                                <  SRR4302540.9026556/94‑1 (MQ=60)

                                 CTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGC                                                              <  SRR4302540.9259447/94‑1 (MQ=60)

                                 CTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGC                                                              >  SRR4302540.5338449/1‑94 (MQ=60)

                                 CTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGC                                                              >  SRR4302540.374611/1‑94 (MQ=60)

                                  TGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCC                                                             >  SRR4302540.10158046/1‑94 (MQ=60)

                                   GGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCC                                                            >  SRR4302540.7952160/1‑94 (MQ=60)

                                   GGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCC                                                            >  SRR4302540.7758408/1‑94 (MQ=60)

                                   GGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATA‑TTGACCGCCCC                                                           >  SRR4302540.8866033/1‑94 (MQ=60)

                                    GAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCC                                                           <  SRR4302540.5302524/94‑1 (MQ=60)

                                    GAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCC                                                           >  SRR4302540.6254267/1‑94 (MQ=60)

                                       GGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATG                                                        >  SRR4302540.2965868/1‑94 (MQ=60)

                                       GGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATG                                                        >  SRR4302540.8194696/1‑94 (MQ=60)

                                       GGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATG                                                        >  SRR4302540.5802383/1‑94 (MQ=60)

                                        GAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGC                                                       >  SRR4302540.2930993/1‑94 (MQ=60)

                                         AACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCA                                                      >  SRR4302540.2631400/1‑94 (MQ=60)

                                         AACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCA                                                      >  SRR4302540.4864409/1‑94 (MQ=60)

                                            ACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAA                                                   >  SRR4302540.6134552/1‑94 (MQ=60)

                                            ACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAA                                                   >  SRR4302540.4160988/1‑94 (MQ=60)

                                            ACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAA                                                   <  SRR4302540.3832147/94‑1 (MQ=60)

                                              CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACG                                                 <  SRR4302540.5372527/94‑1 (MQ=60)

                                              CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACG                                                 <  SRR4302540.218489/94‑1 (MQ=60)

                                              CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACG                                                 <  SRR4302540.7898914/94‑1 (MQ=60)

                                               GCAAACTGCCCCGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGG                                                <  SRR4302540.9900191/94‑1 (MQ=60)

                                               GCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGG                                                >  SRR4302540.238143/1‑94 (MQ=60)

                                                CAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGA                                               <  SRR4302540.4495136/94‑1 (MQ=60)

                                                CAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGA                                               <  SRR4302540.5541512/94‑1 (MQ=60)

                                                 AAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAA                                              <  SRR4302540.4457351/94‑1 (MQ=60)

                                                 AAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAA                                              <  SRR4302540.4457346/94‑1 (MQ=60)

                                                   ACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAG                                            <  SRR4302540.5219538/94‑1 (MQ=60)

                                                    cgtCCCCGCTTCGATAAGGAGCACCTGATCAGTCTCTGCACCGTGGTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGG                                           <  SRR4302540.10003844/91‑1 (MQ=60)

                                                    CTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGG                                           >  SRR4302540.8564567/1‑94 (MQ=60)

                                                      GCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCG                                         >  SRR4302540.3703064/1‑94 (MQ=60)

                                                      GCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCG                                         <  SRR4302540.1843552/94‑1 (MQ=60)

                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCTCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302540.4251891/1‑94 (MQ=60)

                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       <  SRR4302540.8241848/94‑1 (MQ=60)

                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302540.5355986/1‑94 (MQ=60)

                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302540.6762026/1‑94 (MQ=60)

                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302540.7444646/1‑94 (MQ=60)

                                                         CCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGG                                      <  SRR4302540.251634/94‑1 (MQ=60)

                                                         CCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGG                                      <  SRR4302540.9470409/94‑1 (MQ=60)

                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     <  SRR4302540.8574960/94‑1 (MQ=60)

                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     <  SRR4302540.4387664/94‑1 (MQ=60)

                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     <  SRR4302540.9887034/94‑1 (MQ=60)

                                                           GCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCGTAACGGAAAGGCGTGGGT                                    <  SRR4302540.6962668/94‑1 (MQ=60)

                                                            CTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATCTTTGACCGCCCCATGCATAACGGAAAGGCGTagtaa                                   >  SRR4302540.423050/1‑89 (MQ=60)

                                                            CTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTA                                   <  SRR4302540.8009278/94‑1 (MQ=60)

                                                            CTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTA                                   >  SRR4302540.6303815/1‑94 (MQ=60)

                                                            CTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTA                                   >  SRR4302540.6303813/1‑94 (MQ=60)

                                                               CGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAC                                >  SRR4302540.7565656/1‑94 (MQ=60)

                                                               CGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAC                                >  SRR4302540.821402/1‑94 (MQ=60)

                                                                GATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCTGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACC                               >  SRR4302540.6622792/1‑94 (MQ=60)

                                                                GATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACC                               <  SRR4302540.6166585/94‑1 (MQ=60)

                                                                GATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACC                               <  SRR4302540.6539150/94‑1 (MQ=60)

                                                                 ATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCC                              <  SRR4302540.3866579/94‑1 (MQ=60)

                                                                   AAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCC                            <  SRR4302540.9555960/94‑1 (MQ=60)

                                                                   AAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCC                            >  SRR4302540.1644686/1‑94 (MQ=60)

                                                                    AGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCG                           >  SRR4302540.7115602/1‑94 (MQ=60)

                                                                    AGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCG                           <  SRR4302540.2208122/94‑1 (MQ=60)

                                                                     GGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGG                          <  SRR4302540.8693304/94‑1 (MQ=60)

                                                                      GATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGG                         <  SRR4302540.1076787/94‑1 (MQ=60)

                                                                      GATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGG                         <  SRR4302540.8456758/94‑1 (MQ=60)

                                                                      GATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGG                         <  SRR4302540.8245139/94‑1 (MQ=60)

                                                                      GATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGG                         <  SRR4302540.5309793/94‑1 (MQ=60)

                                                                       ATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGC                        >  SRR4302540.4802490/1‑94 (MQ=60)

                                                                       ATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGC                        <  SRR4302540.6342866/94‑1 (MQ=60)

                                                                        TCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGTGCG                       >  SRR4302540.6454460/1‑94 (MQ=60)

                                                                          ACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCG                     >  SRR4302540.6046488/1‑94 (MQ=60)

                                                                          ACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCCCG                     >  SRR4302540.2659987/1‑94 (MQ=60)

                                                                             TGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTC                  <  SRR4302540.52944/94‑1 (MQ=60)

                                                                             TGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTC                  >  SRR4302540.6008734/1‑94 (MQ=60)

                                                                               ATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCT                >  SRR4302540.1517593/1‑94 (MQ=60)

                                                                               ATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCT                >  SRR4302540.147498/1‑94 (MQ=60)

                                                                               ATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCT                <  SRR4302540.4667968/94‑1 (MQ=60)

                                                                               ATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCT                >  SRR4302540.1517594/1‑94 (MQ=60)

                                                                                TCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTT               <  SRR4302540.113593/94‑1 (MQ=60)

                                                                                 CAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTT              >  SRR4302540.8030077/1‑94 (MQ=60)

                                                                                  AGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTA             >  SRR4302540.335887/1‑94 (MQ=60)

                                                                                   GGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAT            <  SRR4302540.9918676/94‑1 (MQ=60)

                                                                                   GGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAT            <  SRR4302540.753159/94‑1 (MQ=60)

                                                                                   GGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAT            <  SRR4302540.2521761/94‑1 (MQ=60)

                                                                                    GCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATC           <  SRR4302540.4666000/94‑1 (MQ=60)

                                                                                     CTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCGTAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCA          <  SRR4302540.7269585/94‑1 (MQ=60)

                                                                                     CTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCA          >  SRR4302540.1334689/1‑94 (MQ=60)

                                                                                       CTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTCTCAAG        >  SRR4302540.8655241/1‑94 (MQ=60)

                                                                                       CTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAG        >  SRR4302540.3097321/1‑94 (MQ=60)

                                                                                       CTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAG        >  SRR4302540.1004938/1‑94 (MQ=60)

                                                                                         GCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT      <  SRR4302540.3688295/94‑1 (MQ=60)

                                                                                         GCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT      <  SRR4302540.9758817/94‑1 (MQ=60)

                                                                                           ACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGA    <  SRR4302540.8050711/94‑1 (MQ=60)

                                                                                           ACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGA    >  SRR4302540.4176050/1‑94 (MQ=60)

                                                                                           ACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGA    >  SRR4302540.411645/1‑94 (MQ=60)

                                                                                            CCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAGCCCCCGGGCGCGTTCCTTTATCAAGATGAC   <  SRR4302540.5513940/94‑1 (MQ=60)

                                                                                            CCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGAC   <  SRR4302540.1196082/94‑1 (MQ=60)

                                                                                             CGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGATG  <  SRR4302540.2970174/94‑1 (MQ=60)

CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACG  >  NC_000913/1877702‑1877888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`