Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,485	T→C	E170G (GAA→GGA)	b1838 ←	protein phosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,485	0	T	C	99.6%	877.0 / ‑4.8	275	E170G (GAA→GGA)	b1838	protein phosphatase 1
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (159/115); total (159/116)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 284 total aligned reads displayed.

ATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA  >  NC_000913/1920394‑1920574

 tGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCa                                                                                         >  1:4817913/1‑93 (MQ=255)

   cAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCAcc                                                                                       <  1:8208653/93‑1 (MQ=255)

    aGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCt                                                                                      >  2:3857442/1‑93 (MQ=255)

      gTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTaa                                                                                    <  2:8690582/93‑1 (MQ=255)

      gTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTaa                                                                                    <  1:2170096/93‑1 (MQ=255)

       ttGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAAt                                                                                   >  2:7927094/1‑93 (MQ=255)

         gCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATcg                                                                                 <  2:4714320/93‑1 (MQ=255)

          ccAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATcgc                                                                                >  1:5739175/1‑93 (MQ=255)

              tatCCACGCGATGTCGCAACGGTTTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGc                                                                            <  2:5530866/93‑1 (MQ=255)

               atCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCg                                                                           >  2:6537820/1‑93 (MQ=255)

                 ccACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGc                                                                         >  1:4508393/1‑93 (MQ=255)

                  cACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCt                                                                        >  1:8290630/1‑93 (MQ=255)

                   aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  2:3375878/1‑93 (MQ=255)

                   aCGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       >  2:1659491/1‑93 (MQ=255)

                   aCGCGATGTCGCAACAGTGTATGTCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTc                                                                       <  2:6712419/93‑1 (MQ=255)

                    cgcgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTcc                                                                      >  1:3569665/1‑93 (MQ=255)

                    cgcgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTcc                                                                      >  2:5031881/1‑93 (MQ=255)

                     gcgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCca                                                                     >  2:9336858/1‑93 (MQ=255)

                     gcgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCca                                                                     <  2:3121771/93‑1 (MQ=255)

                      cgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCcac                                                                    <  1:9509438/93‑1 (MQ=255)

                      cgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCcac                                                                    <  2:3764636/93‑1 (MQ=255)

                      cgATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCcac                                                                    >  2:5782230/1‑93 (MQ=255)

                        aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACaa                                                                  >  2:4031351/1‑93 (MQ=255)

                        aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACaa                                                                  >  1:2709810/1‑93 (MQ=255)

                        aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACaa                                                                  >  1:2410206/1‑93 (MQ=255)

                        aTGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACaa                                                                  <  2:9716005/93‑1 (MQ=255)

                         tGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAg                                                                 >  2:2857398/1‑93 (MQ=255)

                           tCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAc                                                               >  1:1088522/1‑93 (MQ=255)

                           tCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAc                                                               <  1:5959974/93‑1 (MQ=255)

                            cGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAcc                                                              <  1:4057507/93‑1 (MQ=255)

                             gCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCt                                                             >  1:70239/1‑93 (MQ=255)

                             gCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCt                                                             >  2:8293165/1‑93 (MQ=255)

                              cAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACACGACCTg                                                            >  2:2631314/1‑93 (MQ=255)

                               aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           >  1:144203/1‑93 (MQ=255)

                               aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           <  2:2983188/93‑1 (MQ=255)

                               aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           >  2:1093196/1‑93 (MQ=255)

                               aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           <  2:3600857/93‑1 (MQ=255)

                               aaCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGa                                                           >  1:3024637/1‑93 (MQ=255)

                                aCGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAt                                                          >  1:4379781/1‑93 (MQ=255)

                                 cGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATg                                                         >  1:4340412/1‑93 (MQ=255)

                                     gtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaaa                                                     <  2:5394061/93‑1 (MQ=255)

                                     gtATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCaaa                                                     <  2:2472638/93‑1 (MQ=255)

                                       aTGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATc                                                   <  2:455593/93‑1 (MQ=255)

                                        tGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCTTCCACCTATTCGCGAGGCGCTACACAAGACCTTATGCACATCa                                                  >  2:4136522/1‑93 (MQ=255)

                                        tGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCa                                                  >  1:3301874/1‑93 (MQ=255)

                                          aCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGCCCTGATGCAAATCAAc                                                <  2:198756/93‑1 (MQ=255)

                                          aCCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAAc                                                <  1:289339/93‑1 (MQ=255)

                                            cAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGt                                              <  2:6069201/93‑1 (MQ=255)

                                             aaaCCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTc                                             >  2:6107719/1‑93 (MQ=255)

                                                  aGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCtttt                                        <  2:1505989/93‑1 (MQ=255)

                                                  aGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCtttt                                        <  2:8763/93‑1 (MQ=255)

                                                   gAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAAGGTCCTTTTg                                       >  2:4642704/1‑93 (MQ=255)

                                                   gAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTg                                       <  2:7098777/93‑1 (MQ=255)

                                                   gAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTg                                       >  1:9566218/1‑93 (MQ=255)

                                                   gAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTg                                       >  1:7825605/1‑93 (MQ=255)

                                                    aaaTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGc                                      >  1:6667265/1‑93 (MQ=255)

                                                      aTGATCAGCTCTTGTAATTCCCGGCCCTTTTTGCCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCa                                    <  2:6768773/93‑1 (MQ=255)

                                                      aTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCa                                    <  1:9863929/93‑1 (MQ=255)

                                                      aTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCa                                    >  1:2779706/1‑93 (MQ=255)

                                                      aTGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCa                                    <  1:3878172/93‑1 (MQ=255)

                                                       tGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAt                                   >  2:1867066/1‑93 (MQ=255)

                                                       tGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAt                                   <  2:2087207/93‑1 (MQ=255)

                                                         aTCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTc                                 >  2:6289103/1‑93 (MQ=255)

                                                          tCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCa                                >  2:1008167/1‑93 (MQ=255)

                                                           cAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAt                               >  1:8935684/1‑93 (MQ=255)

                                                            aGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATa                              <  1:10009582/93‑1 (MQ=255)

                                                             gCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaa                             <  1:8909131/93‑1 (MQ=255)

                                                              cACCTTTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaaa                            <  2:3514091/93‑1 (MQ=255)

                                                              cACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATaaa                            <  1:8426793/93‑1 (MQ=255)

                                                                 cTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTGCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcat                         <  1:6895847/93‑1 (MQ=255)

                                                                 cTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcat                         <  2:6147289/93‑1 (MQ=255)

                                                                 cTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcat                         >  1:203800/1‑93 (MQ=255)

                                                                 cTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcat                         >  1:9181682/1‑93 (MQ=255)

                                                                  tGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatc                        >  2:1016111/1‑93 (MQ=255)

                                                                  tGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatc                        >  1:5440383/1‑93 (MQ=255)

                                                                  tGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatc                        >  1:9286358/1‑93 (MQ=255)

                                                                   gTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAcatca                       >  1:5572977/1‑93 (MQ=255)

                                                                      aTTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCt                    <  1:7655368/93‑1 (MQ=255)

                                                                       ttCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTg                   >  1:6175400/1‑93 (MQ=255)

                                                                        tCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTgg                  <  2:3295971/93‑1 (MQ=255)

                                                                         cccTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGa                 >  1:6607720/1‑93 (MQ=255)

                                                                          ccTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGAt                <  2:6683724/93‑1 (MQ=255)

                                                                           cTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATa               >  2:9831467/1‑93 (MQ=255)

                                                                           cTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATa               >  1:307516/1‑93 (MQ=255)

                                                                            tGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATaa              >  1:6354086/1‑93 (MQ=255)

                                                                            tGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATaa              >  2:6697850/1‑93 (MQ=255)

                                                                            tGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATaa              >  2:9139711/1‑93 (MQ=255)

                                                                            tGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAAATCATCTGGATaa              >  2:9574510/1‑93 (MQ=255)

                                                                             gCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAt             >  1:7952927/1‑93 (MQ=255)

                                                                               ccTTTTTGGCGTCCACCTAATCGCGAGTGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCg           >  2:514236/1‑93 (MQ=255)

                                                                               ccTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCg           >  1:511243/1‑93 (MQ=255)

                                                                               ccTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCg           <  1:1647587/93‑1 (MQ=255)

                                                                                cTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCgg          <  1:7474412/93‑1 (MQ=255)

                                                                                 tttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGc         >  2:5247798/1‑93 (MQ=255)

                                                                                 tttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGc         <  1:116844/93‑1 (MQ=255)

                                                                                  ttttGGCGTCCCCCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCa        <  1:8153633/93‑1 (MQ=255)

                                                                                  ttttGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCa        <  2:7796972/93‑1 (MQ=255)

                                                                                      ggCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAg    >  1:1517519/1‑93 (MQ=255)

                                                                                       gCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGc   <  1:8628871/93‑1 (MQ=255)

                                                                                        cGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCa  <  1:7138594/93‑1 (MQ=255)

ATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA  >  NC_000913/1920394‑1920574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  >  NC_000913/1920392‑1920578

TAATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTC                                                                                               <  SRR4302540.9918380/94‑1 (MQ=60)

TAATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTC                                                                                               <  SRR4302540.9918379/94‑1 (MQ=60)

 AATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCC                                                                                              >  SRR4302540.5998541/1‑94 (MQ=60)

  ATGGAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCA                                                                                             <  SRR4302540.3068570/94‑1 (MQ=60)

  ATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCA                                                                                             >  SRR4302540.4869485/1‑94 (MQ=60)

  ATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCA                                                                                             <  SRR4302540.6118284/94‑1 (MQ=60)

     CAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCT                                                                                          <  SRR4302540.8304052/94‑1 (MQ=60)

       GGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAA                                                                                        >  SRR4302540.113451/1‑94 (MQ=60)

       GGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAA                                                                                        >  SRR4302540.1550445/1‑94 (MQ=60)

        gtttcccatagtCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAAT                                                                                       <  SRR4302540.2193499/82‑1 (MQ=60)

        GTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAAT                                                                                       <  SRR4302540.10102783/94‑1 (MQ=60)

        GTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAAT                                                                                       <  SRR4302540.2193500/94‑1 (MQ=60)

         GTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATC                                                                                      <  SRR4302540.2852987/94‑1 (MQ=60)

          TGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCG                                                                                     <  SRR4302540.8070944/94‑1 (MQ=60)

           GCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGC                                                                                    >  SRR4302540.5802437/1‑94 (MQ=60)

             CAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGA                                                                                  <  SRR4302540.2450372/94‑1 (MQ=60)

              AATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAG                                                                                 >  SRR4302540.6891679/1‑94 (MQ=60)

               ATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGC                                                                                >  SRR4302540.8383339/1‑94 (MQ=60)

               ATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGC                                                                                >  SRR4302540.8694168/1‑94 (MQ=60)

                 ATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGG                                                                              >  SRR4302540.4454570/1‑94 (MQ=60)

                  TCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGC                                                                             >  SRR4302540.4557246/1‑94 (MQ=60)

                   CCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCT                                                                            >  SRR4302540.8386908/1‑94 (MQ=60)

                   CCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCT                                                                            >  SRR4302540.4921868/1‑94 (MQ=60)

                   CCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCT                                                                            >  SRR4302540.9371958/1‑94 (MQ=60)

                     ACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCC                                                                          >  SRR4302540.3608658/1‑94 (MQ=60)

                      CGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCA                                                                         >  SRR4302540.10160432/1‑94 (MQ=60)

                       GCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCAC                                                                        <  SRR4302540.7170735/94‑1 (MQ=60)

                       GCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCAC                                                                        <  SRR4302540.5618622/94‑1 (MQ=60)

                        CGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACA                                                                       <  SRR4302540.9622084/94‑1 (MQ=60)

                         GATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAA                                                                      >  SRR4302540.2435925/1‑94 (MQ=60)

                         GATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAA                                                                      >  SRR4302540.2436191/1‑94 (MQ=60)

                         GATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAA                                                                      >  SRR4302540.2739032/1‑94 (MQ=60)

                         GATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAA                                                                      >  SRR4302540.2739033/1‑94 (MQ=60)

                          GTGTCGCTACGGTGTATGACCAAACCAGAAATGATCAGCACCTGGAATTCCCTGCCCTTTTTGGCGTCAACCTAATCGCGAGCGGCTCCACAAG                                                                     <  SRR4302540.1706597/94‑1 (MQ=60)

                           TGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGA                                                                    <  SRR4302540.9167307/94‑1 (MQ=60)

                            GTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAC                                                                   <  SRR4302540.4798007/94‑1 (MQ=60)

                            GTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAC                                                                   <  SRR4302540.4406061/94‑1 (MQ=60)

                            GTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGAC                                                                   >  SRR4302540.1099691/1‑94 (MQ=60)

                             TCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACC                                                                  <  SRR4302540.6025900/94‑1 (MQ=60)

                              CGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCT                                                                 <  SRR4302540.6814149/94‑1 (MQ=60)

                              CGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCT                                                                 >  SRR4302540.70951/1‑94 (MQ=60)

                              CGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCT                                                                 <  SRR4302540.4101254/94‑1 (MQ=60)

                              CGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCT                                                                 >  SRR4302540.8344168/1‑94 (MQ=60)

                               GCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTG                                                                <  SRR4302540.2294966/94‑1 (MQ=60)

                                CAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGA                                                               >  SRR4302540.6716610/1‑94 (MQ=60)

                                CAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGA                                                               >  SRR4302540.3057862/1‑94 (MQ=60)

                                CAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGA                                                               >  SRR4302540.145705/1‑94 (MQ=60)

                                 AACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAT                                                              <  SRR4302540.5682522/94‑1 (MQ=60)

                                 AACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAT                                                              >  SRR4302540.4427331/1‑94 (MQ=60)

                                 AACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAT                                                              <  SRR4302540.8256218/94‑1 (MQ=60)

                                  ACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATG                                                             >  SRR4302540.4387662/1‑94 (MQ=60)

                                    GGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCA                                                           >  SRR4302540.7146676/1‑94 (MQ=60)

                                    GGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCA                                                           >  SRR4302540.7719300/1‑94 (MQ=60)

                                    GGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCA                                                           <  SRR4302540.1524033/94‑1 (MQ=60)

                                     GTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAA                                                          >  SRR4302540.6542093/1‑94 (MQ=60)

                                     GTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAA                                                          >  SRR4302540.8941715/1‑94 (MQ=60)

                                     GTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAA                                                          >  SRR4302540.9256534/1‑94 (MQ=60)

                                      TGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAA                                                         >  SRR4302540.3000321/1‑94 (MQ=60)

                                      TGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAA                                                         >  SRR4302540.4533034/1‑94 (MQ=60)

                                       GTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAAT                                                        >  SRR4302540.4052476/1‑94 (MQ=60)

                                         ATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCA                                                      >  SRR4302540.3935979/1‑94 (MQ=60)

                                         ATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCA                                                      >  SRR4302540.3338020/1‑94 (MQ=60)

                                            ACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACG                                                   >  SRR4302540.9678105/1‑94 (MQ=60)

                                            ACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACG                                                   <  SRR4302540.292198/94‑1 (MQ=60)

                                             CCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGT                                                  <  SRR4302540.853715/94‑1 (MQ=60)

                                             CCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGT                                                  <  SRR4302540.5578205/94‑1 (MQ=60)

                                             CCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGT                                                  >  SRR4302540.2772022/1‑94 (MQ=60)

                                               AAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCC                                                >  SRR4302540.4106608/1‑94 (MQ=60)

                                               AAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCC                                                <  SRR4302540.5083299/94‑1 (MQ=60)

                                                 ACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTT                                              >  SRR4302540.2071631/1‑94 (MQ=60)

                                                 ACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTT                                              >  SRR4302540.1492193/1‑94 (MQ=60)

                                                 ACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTT                                              >  SRR4302540.8928632/1‑94 (MQ=60)

                                                  CCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTT                                             >  SRR4302540.9487118/1‑94 (MQ=60)

                                                   CAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAtaccgaatcacaatcacgcgcctttt                                            >  SRR4302540.3628866/1‑68 (MQ=60)

                                                   CAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTT                                            <  SRR4302540.9637994/94‑1 (MQ=60)

                                                   CAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTT                                            <  SRR4302540.2920484/94‑1 (MQ=60)

                                                   CAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTT                                            <  SRR4302540.7017115/94‑1 (MQ=60)

                                                   CAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTT                                            >  SRR4302540.6565512/1‑94 (MQ=60)

                                                    AGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTG                                           >  SRR4302540.7917054/1‑94 (MQ=60)

                                                    AGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTG                                           >  SRR4302540.9679274/1‑94 (MQ=60)

                                                    AGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTG                                           <  SRR4302540.8490361/94‑1 (MQ=60)

                                                     GAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGC                                          >  SRR4302540.6740952/1‑94 (MQ=60)

                                                     GAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGC                                          >  SRR4302540.2792003/1‑94 (MQ=60)

                                                     GAAATGATCACCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGC                                          >  SRR4302540.10008372/1‑94 (MQ=60)

                                                       AATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCA                                        >  SRR4302540.2809739/1‑94 (MQ=60)

                                                        ATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAT                                       <  SRR4302540.7726336/94‑1 (MQ=60)

                                                        ATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAT                                       <  SRR4302540.9979158/94‑1 (MQ=60)

                                                        ATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAT                                       <  SRR4302540.3920318/94‑1 (MQ=60)

                                                        ATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCAT                                       <  SRR4302540.5353417/94‑1 (MQ=60)

                                                         TGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATT                                      <  SRR4302540.7943438/94‑1 (MQ=60)

                                                          GATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTC                                     >  SRR4302540.7257684/1‑94 (MQ=60)

                                                           ATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCA                                    <  SRR4302540.636946/94‑1 (MQ=60)

                                                            TCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAT                                   <  SRR4302540.2978535/94‑1 (MQ=60)

                                                            TCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCAT                                   >  SRR4302540.9040245/1‑94 (MQ=60)

                                                              AGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAA                                 <  SRR4302540.10126139/94‑1 (MQ=60)

                                                              AGCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAA                                 <  SRR4302540.3212842/94‑1 (MQ=60)

                                                               GCACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAA                                <  SRR4302540.9012295/94‑1 (MQ=60)

                                                                CACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAC                               <  SRR4302540.8524621/94‑1 (MQ=60)

                                                                CACCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAAC                               <  SRR4302540.4384577/94‑1 (MQ=60)

                                                                  CCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACAT                             >  SRR4302540.205803/1‑94 (MQ=60)

                                                                  CCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACAT                             >  SRR4302540.9291802/1‑94 (MQ=60)

                                                                  CCTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACAT                             <  SRR4302540.3873827/94‑1 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTGCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            <  SRR4302540.6972380/94‑1 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATACACATC                            >  SRR4302540.9397332/1‑94 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            <  SRR4302540.3450119/94‑1 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            >  SRR4302540.5500240/1‑94 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            >  SRR4302540.5430809/1‑94 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            >  SRR4302540.1104065/1‑94 (MQ=60)

                                                                   CTGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATC                            >  SRR4302540.9397331/1‑94 (MQ=60)

                                                                    TGTAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCA                           >  SRR4302540.5634336/1‑94 (MQ=60)

                                                                      TAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATC                         <  SRR4302540.7745434/94‑1 (MQ=60)

                                                                      TAATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATC                         <  SRR4302540.7745433/94‑1 (MQ=60)

                                                                        ATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTG                       >  SRR4302540.6160691/1‑94 (MQ=60)

                                                                        ATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTG                       <  SRR4302540.7741554/94‑1 (MQ=60)

                                                                        ATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTG                       >  SRR4302540.6244198/1‑94 (MQ=60)

                                                                        ATTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTG                       >  SRR4302540.6716179/1‑94 (MQ=60)

                                                                         TTCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGG                      <  SRR4302540.1394838/94‑1 (MQ=60)

                                                                          TCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGTTCCACAAGCCCTGATGCAAATCAACGTGCTTTTGCCATTCATAAACATCATCTGGA                     >  SRR4302540.3206476/1‑94 (MQ=60)

                                                                          TCCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGA                     >  SRR4302540.6680885/1‑94 (MQ=60)

                                                                          TCCCTGCCCTTTTTGGCGCCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGA                     <  SRR4302540.8284757/94‑1 (MQ=60)

                                                                           CCCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGAT                    <  SRR4302540.8389606/94‑1 (MQ=60)

                                                                            CCTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATA                   >  SRR4302540.310573/1‑94 (MQ=60)

                                                                             CTGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAA                  >  SRR4302540.6424848/1‑94 (MQ=60)

                                                                              TGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAT                 >  SRR4302540.8045633/1‑94 (MQ=60)

                                                                              TGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAT                 <  SRR4302540.3403523/94‑1 (MQ=60)

                                                                              TGCCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAAT                 <  SRR4302540.6193050/94‑1 (MQ=60)

                                                                                CCTTTTTTTGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGAGGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCG               <  SRR4302540.3695206/94‑1 (MQ=60)

                                                                                CCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCG               >  SRR4302540.1573764/1‑94 (MQ=60)

                                                                                CCCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCG               >  SRR4302540.516736/1‑94 (MQ=60)

                                                                                 CCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGG              <  SRR4302540.1665566/94‑1 (MQ=60)

                                                                                 CCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGG              <  SRR4302540.9014516/94‑1 (MQ=60)

                                                                                 CCTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGG              <  SRR4302540.2048784/94‑1 (MQ=60)

                                                                                  CTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGC             <  SRR4302540.7557818/94‑1 (MQ=60)

                                                                                  CTTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGANNATCNGC             >  SRR4302540.5783295/1‑94 (MQ=60)

                                                                                   TTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCA            <  SRR4302540.118101/94‑1 (MQ=60)

                                                                                   TTTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCA            >  SRR4302540.629274/1‑94 (MQ=60)

                                                                                    TTTTGGCGTCCCCCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCAT           <  SRR4302540.8248299/94‑1 (MQ=60)

                                                                                    TTTTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCAT           >  SRR4302540.7511732/1‑94 (MQ=60)

                                                                                     TTGGGCGTGCACCTAATCGCGAGCTTCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATG          <  SRR4302540.9869059/94‑1 (MQ=60)

                                                                                      TTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGA         <  SRR4302540.4758747/94‑1 (MQ=60)

                                                                                      TTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGA         <  SRR4302540.582126/94‑1 (MQ=60)

                                                                                      TTGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGA         <  SRR4302540.9359741/94‑1 (MQ=60)

                                                                                       TGGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAG        >  SRR4302540.1534305/1‑94 (MQ=60)

                                                                                        GGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGC       <  SRR4302540.1229293/94‑1 (MQ=60)

                                                                                        GGCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGC       <  SRR4302540.5668816/94‑1 (MQ=60)

                                                                                         GCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA      >  SRR4302540.9820472/1‑94 (MQ=60)

                                                                                         GCGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCA      <  SRR4302540.8729100/94‑1 (MQ=60)

                                                                                          CGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGCGCAA     >  SRR4302540.3260985/1‑94 (MQ=60)

                                                                                          CGTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAA     <  SRR4302540.7218121/94‑1 (MQ=60)

                                                                                           GTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATGGGCATGAGCAAT    >  SRR4302540.1517192/1‑94 (MQ=60)

                                                                                           GTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAAT    <  SRR4302540.3545815/94‑1 (MQ=60)

                                                                                           GTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAAT    <  SRR4302540.3520821/94‑1 (MQ=60)

                                                                                           GTCCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAAA    >  SRR4302540.121960/1‑94 (MQ=60)

                                                                                             CCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  <  SRR4302540.9515482/94‑1 (MQ=60)

                                                                                             CCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  <  SRR4302540.1386625/94‑1 (MQ=60)

                                                                                             CCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  <  SRR4302540.7487927/94‑1 (MQ=60)

                                                                                             CCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  <  SRR4302540.2638126/94‑1 (MQ=60)

TAATGCAGGTTGCCAATATCCACGCGATGTCGCAACGGTGTATGACCAAACCAGAAATGATCAGCACCTGTAATTCCCTGCCCTTTTTGGCGTTCACCTAATCGCGAGCGGCTCCACAAGACCTGATGCAAATCAACGTCCTTTTGCCATTCATAAACATCATCTGGATAATCGGCATGAGCAATAA  >  NC_000913/1920392‑1920578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`