Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,999,772 T→C H4R (CAC→CGC)  b1922 ← sigma factor 28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,7720TC100.0% 903.8 / NA 282H4R (CAC→CGC) b1922sigma factor 28
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (162/120);  total (162/120)

Only 100 of 291 total aligned reads displayed.

AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861
                                                                                          |                                                                                         
aGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAgcgt                                                                                         >  2:189387/1‑93 (MQ=255)
 gATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGtt                                                                                        >  2:4234963/1‑93 (MQ=255)
 gATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGtt                                                                                        >  1:48664/1‑93 (MQ=255)
  aTCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGttt                                                                                       >  2:6392767/1‑93 (MQ=255)
    cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     <  1:8798973/93‑1 (MQ=255)
    cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     <  2:4320067/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  1:409039/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  2:9499753/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  1:1791461/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  >  2:8025152/1‑93 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  1:4943799/93‑1 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 <  2:360975/93‑1 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 <  1:8711041/93‑1 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 >  1:6062815/1‑93 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               <  1:8181702/93‑1 (MQ=255)
           ttCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTac                                                                              >  2:7214775/1‑93 (MQ=255)
             ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCATTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  1:9728834/93‑1 (MQ=255)
             ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  1:4441529/93‑1 (MQ=255)
                cGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCtt                                                                         >  2:2207041/1‑93 (MQ=255)
                cGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCtt                                                                         <  2:3344119/93‑1 (MQ=255)
                  cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  2:6247383/93‑1 (MQ=255)
                  cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  2:481683/93‑1 (MQ=255)
                    cgcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGc                                                                     <  1:6719860/93‑1 (MQ=255)
                      cgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCgg                                                                   <  2:270380/93‑1 (MQ=255)
                       gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  2:695549/1‑93 (MQ=255)
                       gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  1:5836843/1‑93 (MQ=255)
                       gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  2:4369629/1‑93 (MQ=255)
                        ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 <  1:6206562/93‑1 (MQ=255)
                          cAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtata                                                               <  1:1700230/93‑1 (MQ=255)
                           aGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATag                                                              <  1:9443483/93‑1 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             >  2:1110412/1‑93 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            >  1:2583350/1‑93 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            >  1:2807010/1‑93 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            <  2:8588273/93‑1 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            >  2:1569363/1‑93 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGAGCCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACCCCTTCAGCGGTATagag                                                            <  2:7482466/93‑1 (MQ=255)
                                 aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  2:8347447/1‑93 (MQ=255)
                                  ccTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAt                                                       >  1:4816320/1‑93 (MQ=255)
                                  ccTGCAGGCGCAATGCTTCGTGACGCACCAGCGCGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGGATAGAGTGAAt                                                       >  2:462470/1‑93 (MQ=255)
                                   cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      >  1:9638048/1‑93 (MQ=255)
                                    tGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTc                                                     >  1:8896819/1‑93 (MQ=255)
                                       aGGCACAATGCTACGAGACGCACCAGCGGGACATAACGCTGCAACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTccca                                                  <  2:8982640/93‑4 (MQ=255)
                                        ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  2:4133702/93‑1 (MQ=255)
                                         gcgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt                                                <  2:3858140/93‑1 (MQ=255)
                                         gcgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt                                                >  1:5491544/1‑93 (MQ=255)
                                         gcgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt                                                >  2:6027588/1‑93 (MQ=255)
                                          cgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATa                                               >  2:7058749/1‑93 (MQ=255)
                                          cgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATa                                               >  2:9230614/1‑93 (MQ=255)
                                           gcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa                                              >  2:5593994/1‑93 (MQ=255)
                                            cAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             >  2:3083358/1‑93 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            <  1:1241364/93‑1 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:9767385/1‑93 (MQ=255)
                                               tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          >  2:77794/1‑93 (MQ=255)
                                                gCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         >  2:10037328/1‑93 (MQ=255)
                                                 cTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGcc                                        <  1:2873258/93‑1 (MQ=255)
                                                  ttCGTGACGCACCAGCGGTACATAACGCTGCCACAGCGAGCGTTTATCCATTACGCCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  2:3962323/93‑1 (MQ=255)
                                                  ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  1:3757920/93‑1 (MQ=255)
                                                    cGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTg                                     >  1:6108999/1‑93 (MQ=255)
                                                     gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    >  1:41602/1‑93 (MQ=255)
                                                      tGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCg                                   <  2:8097826/93‑1 (MQ=255)
                                                       taCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTGCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  <  1:332322/92‑1 (MQ=255)
                                                       gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  1:6441286/1‑93 (MQ=255)
                                                       gACGCACCAGCGGGACATAACACTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGTTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  <  1:2370147/93‑1 (MQ=255)
                                                        aCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGtt                                 <  1:3435764/93‑1 (MQ=255)
                                                           cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              <  1:7043824/93‑1 (MQ=255)
                                                           cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              <  2:5007429/93‑1 (MQ=255)
                                                            aCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTatat                             <  2:1752365/93‑1 (MQ=255)
                                                               aGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          >  1:4667658/1‑93 (MQ=255)
                                                               aGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          >  2:5291234/1‑93 (MQ=255)
                                                               aGCGGGACATAAAGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          <  2:2530325/93‑1 (MQ=255)
                                                                gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         <  1:6639294/93‑1 (MQ=255)
                                                                gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         <  2:8147405/93‑1 (MQ=255)
                                                                 cGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGtt                        >  1:1305663/1‑93 (MQ=255)
                                                                   ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      >  1:10042243/1‑93 (MQ=255)
                                                                   ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      >  2:6507715/1‑93 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:9109872/93‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  1:7639835/93‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  1:9341129/93‑1 (MQ=255)
                                                                     aCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCg                    <  1:1510610/93‑1 (MQ=255)
                                                                      cATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCgg                   <  1:5535815/93‑1 (MQ=255)
                                                                        tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 >  1:853559/1‑93 (MQ=255)
                                                                          aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               >  1:6512341/1‑93 (MQ=255)
                                                                           cGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATTGGCATGa              >  2:8867904/1‑93 (MQ=255)
                                                                            gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             >  2:376093/1‑93 (MQ=255)
                                                                             cTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAtt            <  2:3640805/93‑1 (MQ=255)
                                                                             cTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAtt            <  1:201616/93‑1 (MQ=255)
                                                                             cTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAtt            >  1:3630762/1‑93 (MQ=255)
                                                                              tGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTa           <  2:2062914/93‑1 (MQ=255)
                                                                                ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         >  1:217915/1‑93 (MQ=255)
                                                                                ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         >  2:5169904/1‑93 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        >  1:2551610/1‑93 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        >  2:6697136/1‑93 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        <  2:9595590/93‑1 (MQ=255)
                                                                                  acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  1:5122801/1‑93 (MQ=255)
                                                                                  acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       <  2:9276213/93‑1 (MQ=255)
                                                                                   caGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGt      >  1:7269674/1‑93 (MQ=255)
                                                                                    agcgagcgTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCgct     >  1:7477332/1‑91 (MQ=255)
                                                                                     gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    <  1:1937308/93‑1 (MQ=255)
                                                                                     gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    <  2:40863/93‑1 (MQ=255)
                                                                                       gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  1:2269917/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999679‑1999865
                                                                                             |                                                                                             
AGCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGC                                                                                               >  SRR4302540.2821114/1‑94 (MQ=60)
   AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            >  SRR4302540.2902121/1‑94 (MQ=60)
   AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            >  SRR4302540.49154/1‑94 (MQ=60)
     ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTA                                                                                          >  SRR4302540.2080366/1‑94 (MQ=60)
      TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         >  SRR4302540.6215035/1‑94 (MQ=60)
       CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        <  SRR4302540.8900707/94‑1 (MQ=60)
       CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302540.5583537/1‑94 (MQ=60)
       CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302540.5279699/1‑94 (MQ=60)
        GTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCC                                                                                       >  SRR4302540.8337238/1‑94 (MQ=60)
        GTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCC                                                                                       >  SRR4302540.3431833/1‑94 (MQ=60)
         TCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGCCATAACGCTGCCACAGCGAGCGTTTATCCA                                                                                      >  SRR4302540.620706/1‑94 (MQ=60)
         TCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCA                                                                                      >  SRR4302540.1320834/1‑94 (MQ=60)
          CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302540.413319/94‑1 (MQ=60)
          CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302540.3044378/94‑1 (MQ=60)
          CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302540.1810928/94‑1 (MQ=60)
          CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302540.4997162/94‑1 (MQ=60)
          CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     >  SRR4302540.6130201/1‑94 (MQ=60)
          CAAGTGCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302540.7842498/94‑1 (MQ=60)
           AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    <  SRR4302540.8812162/94‑1 (MQ=60)
            AGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTA                                                                                   <  SRR4302540.126555/94‑1 (MQ=60)
             GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                  <  SRR4302540.8276735/94‑1 (MQ=60)
              TTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACA                                                                                 >  SRR4302540.8981117/1‑94 (MQ=60)
                CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCATTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302540.9843085/94‑1 (MQ=60)
                CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302540.4489661/94‑1 (MQ=60)
                CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               >  SRR4302540.2218599/1‑94 (MQ=60)
                 CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              <  SRR4302540.3635252/94‑1 (MQ=60)
                 CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              >  SRR4302540.4486396/1‑94 (MQ=60)
                 CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              <  SRR4302540.6692717/94‑1 (MQ=60)
                    GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                           >  SRR4302540.2557927/1‑94 (MQ=60)
                    GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                           >  SRR4302540.2557932/1‑94 (MQ=60)
                       CGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGCCGCACCAGCGGGACATACCGCGGCCACAGCGAGGGTTTATCCATTCCCCCTTCAGCG                                                                        >  SRR4302540.7721699/1‑94 (MQ=60)
                       CGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        <  SRR4302540.6794017/94‑1 (MQ=60)
                       CGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        >  SRR4302540.9648536/1‑94 (MQ=60)
                        GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                       <  SRR4302540.599852/94‑1 (MQ=60)
                         CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                      >  SRR4302540.5901375/1‑94 (MQ=60)
                         CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                      >  SRR4302540.5901376/1‑94 (MQ=60)
                          GGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTA                                                                     >  SRR4302540.9305814/1‑94 (MQ=60)
                          GGGCAGTCGAACCTGCAGGCGCAATGCTTCGCGACGCACCAGCGGGACATAACGCTGCCACAGCGACCGCTTATCCATTACACCTTCAGCGGTT                                                                     >  SRR4302540.4012832/1‑94 (MQ=60)
                           GGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTAT                                                                    <  SRR4302540.6275644/94‑1 (MQ=60)
                             CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  <  SRR4302540.6137690/94‑1 (MQ=60)
                             CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  <  SRR4302540.1718639/94‑1 (MQ=60)
                              AGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGA                                                                 <  SRR4302540.9555602/94‑1 (MQ=60)
                               GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                >  SRR4302540.2837325/1‑94 (MQ=60)
                               GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                >  SRR4302540.3767553/1‑94 (MQ=60)
                               GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                >  SRR4302540.2611195/1‑94 (MQ=60)
                                 CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              >  SRR4302540.9778002/1‑94 (MQ=60)
                                  GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGA                                                             >  SRR4302540.4918700/1‑94 (MQ=60)
                                  GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGA                                                             >  SRR4302540.4084683/1‑94 (MQ=60)
                                   AACCTGCAGGCGCAATGCTTCGTGACTCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                            <  SRR4302540.4885303/94‑1 (MQ=60)
                                   AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                            >  SRR4302540.3699316/1‑94 (MQ=60)
                                    ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           >  SRR4302540.4945974/1‑94 (MQ=60)
                                    ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           >  SRR4302540.3493808/1‑94 (MQ=60)
                                    ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           >  SRR4302540.4867871/1‑94 (MQ=60)
                                     CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          >  SRR4302540.9751588/1‑94 (MQ=60)
                                     CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          >  SRR4302540.9751589/1‑94 (MQ=60)
                                     CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          <  SRR4302540.665120/94‑1 (MQ=60)
                                      CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         >  SRR4302540.8999806/1‑94 (MQ=60)
                                      CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         >  SRR4302540.710407/1‑94 (MQ=60)
                                      CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         >  SRR4302540.149146/1‑94 (MQ=60)
                                      CTGCAGGCGCAATGCTGCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         <  SRR4302540.1255659/94‑1 (MQ=60)
                                       TGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCA                                                        >  SRR4302540.6787166/1‑94 (MQ=60)
                                         CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      <  SRR4302540.3234430/94‑1 (MQ=60)
                                           GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    >  SRR4302540.5552004/1‑94 (MQ=60)
                                             CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302540.1753001/94‑1 (MQ=60)
                                             CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302540.1753002/94‑1 (MQ=60)
                                             CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  >  SRR4302540.5266033/1‑94 (MQ=60)
                                              GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302540.4507932/1‑94 (MQ=60)
                                              GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302540.2228499/1‑94 (MQ=60)
                                              GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 <  SRR4302540.6391587/94‑1 (MQ=60)
                                              GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302540.3422421/1‑94 (MQ=60)
                                               CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302540.7233094/94‑1 (MQ=60)
                                                AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               <  SRR4302540.1254032/94‑1 (MQ=60)
                                                AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               >  SRR4302540.5011190/1‑94 (MQ=60)
                                                 ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              >  SRR4302540.5594669/1‑94 (MQ=60)
                                                 ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              >  SRR4302540.9809727/1‑94 (MQ=60)
                                                 ATGCTTCGTGACGCACCAGCGGGACAAAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              <  SRR4302540.706565/94‑1 (MQ=60)
                                                  TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             <  SRR4302540.5662619/94‑1 (MQ=60)
                                                   GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                            <  SRR4302540.645545/94‑1 (MQ=60)
                                                   GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                            >  SRR4302540.4447688/1‑94 (MQ=60)
                                                    CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                           <  SRR4302540.2904492/94‑1 (MQ=60)
                                                     TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302540.5946087/94‑1 (MQ=60)
                                                     TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302540.6566207/94‑1 (MQ=60)
                                                     TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302540.3798915/94‑1 (MQ=60)
                                                     TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          >  SRR4302540.4134810/1‑94 (MQ=60)
                                                      TCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTG                                         >  SRR4302540.6177034/1‑94 (MQ=60)
                                                       CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGC                                        >  SRR4302540.42030/1‑94 (MQ=60)
                                                        GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       <  SRR4302540.7536620/94‑1 (MQ=60)
                                                         TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302540.6512976/1‑94 (MQ=60)
                                                         TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302540.170759/1‑94 (MQ=60)
                                                          TACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTGCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTT                                     <  SRR4302540.335669/94‑1 (MQ=60)
                                                          GACGCACCAGCGGGACATAACACTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGTTATAGAGTGAATTCACGATAAACAGCCCTGCGTT                                     <  SRR4302540.2395678/94‑1 (MQ=60)
                                                           ACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTA                                    <  SRR4302540.3473113/94‑1 (MQ=60)
                                                           ACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTA                                    <  SRR4302540.188375/94‑1 (MQ=60)
                                                              CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302540.7122143/94‑1 (MQ=60)
                                                              CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302540.944416/94‑1 (MQ=60)
                                                               ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                                >  SRR4302540.1984149/1‑94 (MQ=60)
                                                                CCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGA                               >  SRR4302540.4685742/1‑94 (MQ=60)
                                                                 CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              >  SRR4302540.4717618/1‑94 (MQ=60)
                                                                  AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTCTAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             >  SRR4302540.7507880/1‑94 (MQ=60)
                                                                   GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            >  SRR4302540.1319208/1‑94 (MQ=60)
                                                                   GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302540.6712764/94‑1 (MQ=60)
                                                                     GGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAT                          >  SRR4302540.10158995/1‑94 (MQ=60)
                                                                     GGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAT                          >  SRR4302540.10158996/1‑94 (MQ=60)
                                                                     GGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAT                          >  SRR4302540.2799575/1‑94 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302540.10076491/94‑1 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         >  SRR4302540.7579991/1‑94 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         >  SRR4302540.8765229/1‑94 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         >  SRR4302540.7579990/1‑94 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302540.6141812/94‑1 (MQ=60)
                                                                      GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCACTGCGTTATATGAGTTATC                         >  SRR4302540.8765228/1‑94 (MQ=60)
                                                                       GACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCG                        <  SRR4302540.7725809/94‑1 (MQ=60)
                                                                       GACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCG                        <  SRR4302540.9452445/94‑1 (MQ=60)
                                                                        ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       <  SRR4302540.1527300/94‑1 (MQ=60)
                                                                        ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       >  SRR4302540.199959/1‑94 (MQ=60)
                                                                         CATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGC                      <  SRR4302540.5596787/94‑1 (MQ=60)
                                                                         CATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGC                      <  SRR4302540.1939133/94‑1 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.929818/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.2594042/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.862626/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.2387547/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.1557001/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.9999748/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.676870/1‑94 (MQ=60)
                                                                          ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302540.4190439/1‑94 (MQ=60)
                                                                           TAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAT                    <  SRR4302540.8829494/94‑1 (MQ=60)
                                                                            AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302540.6584687/1‑94 (MQ=60)
                                                                            AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   <  SRR4302540.881882/94‑1 (MQ=60)
                                                                            AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   <  SRR4302540.2863252/94‑1 (MQ=60)
                                                                              CGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAT                 >  SRR4302540.2836968/1‑94 (MQ=60)
                                                                               GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                >  SRR4302540.3670502/1‑94 (MQ=60)
                                                                                CTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTA               <  SRR4302540.203604/94‑1 (MQ=60)
                                                                                  GCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATC             >  SRR4302540.4907585/1‑94 (MQ=60)
                                                                                  GCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATC             >  SRR4302540.220035/1‑94 (MQ=60)
                                                                                   CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            >  SRR4302540.7872753/1‑94 (MQ=60)
                                                                                   CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            >  SRR4302540.2579113/1‑94 (MQ=60)
                                                                                    CACAGCGAGCGTTTATCCATTACTGCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           <  SRR4302540.5047125/94‑1 (MQ=60)
                                                                                    CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302540.5178272/1‑94 (MQ=60)
                                                                                    CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302540.4773567/1‑94 (MQ=60)
                                                                                    CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302540.2326049/1‑94 (MQ=60)
                                                                                     ACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGT          <  SRR4302540.9502020/94‑1 (MQ=60)
                                                                                     ACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGT          >  SRR4302540.7350794/1‑94 (MQ=60)
                                                                                      CAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGCT         >  SRR4302540.7560778/1‑94 (MQ=60)
                                                                                        GCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTC       <  SRR4302540.1958413/94‑1 (MQ=60)
                                                                                         CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302540.2294271/1‑94 (MQ=60)
                                                                                         CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302540.8304781/94‑1 (MQ=60)
                                                                                          GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG     >  SRR4302540.103360/1‑94 (MQ=60)
                                                                                          GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG     <  SRR4302540.2119067/94‑1 (MQ=60)
                                                                                          GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG     >  SRR4302540.7843624/1‑94 (MQ=60)
                                                                                            GCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCA   <  SRR4302540.3933682/94‑1 (MQ=60)
                                                                                             CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  SRR4302540.4856185/1‑94 (MQ=60)
                                                                                             CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  <  SRR4302540.8726491/94‑1 (MQ=60)
                                                                                             CGTTTATCCATTACACATTCAGCGATATAGAGTAAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGAATGATTATACGTTTCTGCAG  <  SRR4302540.9044852/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999679‑1999865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑